MC07g_new0302 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g_new0302
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	AMP-binding domain-containing protein
Location	MC07: 10495167 .. 10498532 (+)
RNA-Seq Expression	MC07g_new0302
Synteny	MC07g_new0302

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAGTCCAGAAAATCCACGCGACTTCTCTTACTCTACTTCGGTATTTTCGTTACCCGCCGCTGATGTTGTGGTCGCCGACAACTTGAAAACCGCCGCTGCCATTCAAGGAGCCGACACAGTAATCACCCAAGAAATCTTGGGAGACGATATTTCCCCCGTACCTGGCGCCCACACCGGAACCTCTGAGAATCCAGACAGTTCCTCGGACATTCCGCAGTGTTTTGAAGCGAAGATGGACTACAACAACTGCATTAAAGAAAATGAGAAACTGAAGGAGAATTGTGAAAGGTTGGAGCTCTGTAAAGAAATCAAGGACAGAGAACTTCAACGGTTGAAGATGAATTGTGAAATACTGACTCACAAGATAGAAAAAGACATTGTTATCAAAACCCTCTTGAAATATGCTGAAGCTAGGAACGACTCATTAGAATATGAAATAGGGAGGCTCAGAGCGCTGATTGTCGAAACCACCGGCCATGAGCCGTAAATCGCGACCACCAAGAGTGATGGTGGTCGCGATTTACACATATTGGTATCTTGTAATGTTTTCCTGTTTGGACTGGAAAGAAGCAGAAGGTTTTTGGCTTAGTTTTTGTTTGATGCCTTTGTTGTTTGCTGTTTTTTTTTTTGGTACACTGTTTGCATGACATCGTGGTTACACTTTTGAACTAATGACAGCTTCTGCAGCACTGCCTTTTTTTCTTTTTCTCAGCCATAGTTCTGAATTCCATTTTAGTAATTGATTGTGAATTATTTTCAGCGGGTTTTTGAATGTCTTAGAATACCCTTTACATGAATCTTATAATTGCATAAAGCTTTCTCGCGTAGGGGATAACAGGCTGATGACTCCCAAGAGCTCTTATCGACGGAGTCGTTTGGCATCTCGATGTCGACTCATCACATCCTGGGGTTGAAGAAGGTCCCAAGGGTTCGGTTGTTCGCCGATTCAAGTGGTACGTGAGTTGGGTTTAGAACGTCGTGAGACAACTTGTTTCTTTTGTGTTATATATTTTAACCATAGAACGTCCAATCATGTCAAAAATCAAGAAAATATTATAAGTAAATTGCCAACTCTCTAATTTACATGCTTGACATGTCATGGACACAAAACCCTCCAAACTTTGAAAGCTTCATAGAAAAAATTGTCATAAGAACAAGTTATGATTGAATGTTTGAACCAATAGATTCAGTTTGAGAAGATGGTTGTTTAGTCCGTATTATCGTCTGGATAATGTCATCAAACATCTTGTTTATTTTTGGTTGTTGAAGACTATACTTTTTATTCACAAGGTTCTTGATATTAGAACAGCTAACTATCCCTAGAATCTGCAGGCAAGCATAAGTGGAGGGGGCAGTTTGCCTTCACACGTTGATCAATTTTTTGAGGTTAGATGCTTAAATATGTTTAATGATTGTAAATGAATATGTTCGTTTACAATTGATATGATTGAGCATTCTTGCAGGCAATTGGAATTACAGTGCAGAATGGGTATGGTTTAACAGAATGTTCTCCTCTTATTGCTTTGTATGGAATGCTCATATAATGTCTTAGTTAATTTTGGAGTATATGATTGGCATAGTTTTACAGAGCAATAACAATGTTTCTCAAAGAAATTTTGATATTCCTTACCTGCATCCTCTTACTGTAATAGATCATATACTGTTTCTGCCATTCTTCTTAGTAATGGATGTAATGTAAGTCAATATAATGTGCTAAAGTGATGCTAACCTTAGCAATAAACCTATGATAGATTAGCAATCATTGGTTGTGATTTCCCTTGATCAATAATTGGAAATCGGTCTTGTAAGTTTCAAACTAGTAGCTTTAATCATCCTGATTATCAAATGGCTTTCAAATTATTTTAGGGAGCTTTCTTTCTGTAGATAAAAATTCAACGCACCACTCTCTCTTTGATGTCGTTATGCCCAATTCTTTAAGAATAGGATATTATATACTCATTGTCTCCCTTGCTAAGATTAACTGTGTTATTAGCTCCATTTTGTTTTCAACCTTTTCCATACCTATTGGCAGATTATATGACAGTTATGCAGATTTTAACTTTTAAAGGTTCTTGGTTTGGTTGTACATCCAATTCGGCATACAGAGTTCGGGATTGTAGGTTTTGAAACTGGAGATGTTCTCCCAGCTGGATCAAGAGGCATTGTTGAAGTCAGGGGACCTCAAGTGATGAAAGGTTACTATAAGGTATTTTCATCTCTCGGTCATCTATACACTTCAATTTTCTTATGTTGTACTTTTCTCTATTTGAAATATCATTTTCATCTTAAATATGATAATTAGATTCTACAACTAAGATATCATTTTCATCTTAAATATGATAATTAGATTCTACAACTAAATATGATTGCACTATAGATGATGTATTTGTTATTTTTTCCTTGAAAGTACTAAGCTTTCTTTGAGATAAAATGAAAGAATACAAAAGGCACACGAGAAGATAGCCCTAGCAAGAGGAGTCCAACTAAACCAAAAAGTGACTCCAATCTAACAAATATCTCTCAACCCTCTAAAAATTCTATTGTTTCTCTCCATCCACGAATCCTGGCAAGCCAAAGAAAACATCCCTTATCATGAAAGAGCGAAAAAGTGAGCAACTTCATCAGCACACTACCATCTCTAGGACCAGCGAGGCTGAAACCAAACTCTTAAAGAACAGTGTTCCAAGCCAAGTGAGCAAAATTATCTAGGATTTATAATGACTCTAGTTTTGGTATTCCTCAGGGAATCCCACGTACAGCTGTATAGTTGAATATCTCTCCTATGGAAATCACTCATGTGCATATCTAAAATTATAAAGGGAGTACTTTTATAAATTATACTAAGCAACAAGTTAATGCATCTGTTAGAGTATATATTAGTATATTTTATATTCTTGTGTTAATATTAATTTGTCATTTACCTTCTCCTTATAGGATTAGGTCAACCTTGTATATTTGTCTATATATATAGACTTTAATAATGAGAATACAGAACATTGGATTCTCTCCAACCTTAGTCTCTACATGGTATCAGAGCACTAGGTTTCTTCTATGTGCCCATTAGGATTTTGATGCCCATTAGGGTTTTGTGCCCATTAGGGTTTTGTCCCTTAGGGTTTTTGTTTATTGTTATCCAACACAACTCACTACGACCGCCGTCACCGCCGATCACCGCCGGCCGCCGCCGACCACCACTTCCGGCCGCCGGCCGCCACCGCCGCCGCTGTCGTTTGCTGATTTGCCAACTGTTTTCCGATTGTTGTTCGTAGGTTTCATCTCGCCGCCAAATGCCATCGCCGTGTGCTTTCGTCACCGCCAGTCGCCACCCGTCGTCTTCACCCCTCGGGTTGTCGTGGGGTAG

mRNA sequence

ATGAGCAGTCCAGAAAATCCACGCGACTTCTCTTACTCTACTTCGGTATTTTCGTTACCCGCCGCTGATGTTGTGGTCGCCGACAACTTGAAAACCGCCGCTGCCATTCAAGGAGCCGACACAGTAATCACCCAAGAAATCTTGGGAGACGATATTTCCCCCGTACCTGGCGCCCACACCGGAACCTCTGAGAATCCAGACAGTTCCTCGGACATTCCGCAGTGTTTTGAAGCGAAGATGGACTACAACAACTGCATTAAAGAAAATGAGAAACTGAAGGAGAATTGTGAAAGGTTGGAGCTCTGTAAAGAAATCAAGGACAGAGAACTTCAACGGTTGAAGATGAATTGTGAAATACTGACTCACAAGATAGAAAAAGACATTGTTATCAAAACCCTCTTGAAATATGCTGAAGCTAGGAACGACTCATTAGAATATGAAATAGGGAGGCTCAGAGCGCTGATTGTCGAAACCACCGGCCATGAGCCGTCCCAAGGGTTCGGTTGTTCGCCGATTCAAGTGGCAATTGGAATTACAGTGCAGAATGGGTATGGTTTAACAGAATGTTCTCCTCTTATTGCTTTATCATATACTGTTTCTGCCATTCTTCTTAGTAATGGATGTAATGTTCTTGGTTTGGTTGTACATCCAATTCGGCATACAGAGTTCGGGATTGTAGGTTTTGAAACTGGAGATGTTCTCCCAGCTGGATCAAGAGGCATTGTTGAAGTCAGGGGACCTCAAGTGATGAAAGGTTACTATAAGGGTTTTGTGCCCATTAGGGTTTTGTCCCTTAGGGTTTTTGTTTATTGTTATCCAACACAACTCACTACGACCGCCGTCACCGCCGATCACCGCCGGCCGCCGCCGACCACCACTTCCGGCCGCCGGCCGCCACCGCCGCCGCTGTCGTTTGCTGATTTGCCAACTGTTTTCCGATTGTTGTTCGTAGGTTTCATCTCGCCGCCAAATGCCATCGCCGTGTGCTTTCGTCACCGCCAGTCGCCACCCGTCGTCTTCACCCCTCGGGTTGTCGTGGGGTAG

Coding sequence (CDS)

ATGAGCAGTCCAGAAAATCCACGCGACTTCTCTTACTCTACTTCGGTATTTTCGTTACCCGCCGCTGATGTTGTGGTCGCCGACAACTTGAAAACCGCCGCTGCCATTCAAGGAGCCGACACAGTAATCACCCAAGAAATCTTGGGAGACGATATTTCCCCCGTACCTGGCGCCCACACCGGAACCTCTGAGAATCCAGACAGTTCCTCGGACATTCCGCAGTGTTTTGAAGCGAAGATGGACTACAACAACTGCATTAAAGAAAATGAGAAACTGAAGGAGAATTGTGAAAGGTTGGAGCTCTGTAAAGAAATCAAGGACAGAGAACTTCAACGGTTGAAGATGAATTGTGAAATACTGACTCACAAGATAGAAAAAGACATTGTTATCAAAACCCTCTTGAAATATGCTGAAGCTAGGAACGACTCATTAGAATATGAAATAGGGAGGCTCAGAGCGCTGATTGTCGAAACCACCGGCCATGAGCCGTCCCAAGGGTTCGGTTGTTCGCCGATTCAAGTGGCAATTGGAATTACAGTGCAGAATGGGTATGGTTTAACAGAATGTTCTCCTCTTATTGCTTTATCATATACTGTTTCTGCCATTCTTCTTAGTAATGGATGTAATGTTCTTGGTTTGGTTGTACATCCAATTCGGCATACAGAGTTCGGGATTGTAGGTTTTGAAACTGGAGATGTTCTCCCAGCTGGATCAAGAGGCATTGTTGAAGTCAGGGGACCTCAAGTGATGAAAGGTTACTATAAGGGTTTTGTGCCCATTAGGGTTTTGTCCCTTAGGGTTTTTGTTTATTGTTATCCAACACAACTCACTACGACCGCCGTCACCGCCGATCACCGCCGGCCGCCGCCGACCACCACTTCCGGCCGCCGGCCGCCACCGCCGCCGCTGTCGTTTGCTGATTTGCCAACTGTTTTCCGATTGTTGTTCGTAGGTTTCATCTCGCCGCCAAATGCCATCGCCGTGTGCTTTCGTCACCGCCAGTCGCCACCCGTCGTCTTCACCCCTCGGGTTGTCGTGGGGTAG

Protein sequence

MSSPENPRDFSYSTSVFSLPAADVVVADNLKTAAAIQGADTVITQEILGDDISPVPGAHTGTSENPDSSSDIPQCFEAKMDYNNCIKENEKLKENCERLELCKEIKDRELQRLKMNCEILTHKIEKDIVIKTLLKYAEARNDSLEYEIGRLRALIVETTGHEPSQGFGCSPIQVAIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVLPAGSRGIVEVRGPQVMKGYYKGFVPIRVLSLRVFVYCYPTQLTTTAVTADHRRPPPTTTSGRRPPPPPLSFADLPTVFRLLFVGFISPPNAIAVCFRHRQSPPVVFTPRVVVG

Homology

[Close]

BLAST of MC07g_new0302 vs. ExPASy Swiss-Prot
Match: Q9LK39 (Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AAE16 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.9e-19
Identity = 58/95 (61.05%), Postives = 64/95 (67.37%), Query Frame = 0


Query: 166 GFGCSPIQV-----AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRH 225
           G G  P+ V     AIG+ VQNGYGLTE SP+      VSA  L   CNVLG V HPI+ 
Sbjct: 457 GGGSLPMHVDKFFEAIGVNVQNGYGLTETSPV------VSARRLR--CNVLGSVGHPIKD 516

Query: 226 TEFGIVGFETGDVLPAGSRGIVEVRGPQVMKGYYK 256
           TEF IV  ETG VLP GS+GIV+VRGP VMKGYYK
Sbjct: 517 TEFKIVDHETGTVLPPGSKGIVKVRGPPVMKGYYK 543

[Close]

BLAST of MC07g_new0302 vs. ExPASy Swiss-Prot
Match: Q8W471 (Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AAE15 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.6e-18
Identity = 53/95 (55.79%), Postives = 64/95 (67.37%), Query Frame = 0


Query: 166 GFGCSPIQV-----AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRH 225
           G G  PI V     AIG+ +QNGYGLTE SP++  + T+S       CNVLG   HP+  
Sbjct: 473 GGGSLPIHVDKFFEAIGVILQNGYGLTETSPVVC-ARTLS-------CNVLGSAGHPMHG 532

Query: 226 TEFGIVGFETGDVLPAGSRGIVEVRGPQVMKGYYK 256
           TEF IV  ET +VLP GS+GI++VRGPQVMKGYYK
Sbjct: 533 TEFKIVDPETNNVLPPGSKGIIKVRGPQVMKGYYK 559

[Close]

BLAST of MC07g_new0302 vs. ExPASy Swiss-Prot
Match: P41636 (4-coumarate--CoA ligase OS=Pinus taeda OX=3352 GN=4CL PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.0e-04
Identity = 25/71 (35.21%), Postives = 39/71 (54.93%), Query Frame = 0


Query: 183 GYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVLPAGSRGIV 242
           GYG+TE  P++A++   +           G VV   R+ +  I+  ETG+ LP    G +
Sbjct: 332 GYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVV---RNAQIKILDTETGESLPHNQAGEI 391

Query: 243 EVRGPQVMKGY 254
            +RGP++MKGY
Sbjct: 392 CIRGPEIMKGY 399

[Close]

BLAST of MC07g_new0302 vs. ExPASy Swiss-Prot
Match: A1JPF0 (Bifunctional protein Aas OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) OX=393305 GN=aas PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.0e-04
Identity = 30/110 (27.27%), Postives = 54/110 (49.09%), Query Frame = 0


Query: 146 YEIGRLRALIVETTGHEPSQGFGCSPIQVAIGITVQNGYGLTECSPLIALSYTVSAILLS 205
           Y+  RLR ++    G E          Q   GI +  GYG+TEC+P++A++  ++A +  
Sbjct: 476 YDFARLRYVV---AGAEKLADSTKQIWQDKFGIRILEGYGVTECAPVVAINVPMAAKV-- 535

Query: 206 NGCNVLGLVVHPIRHTEFGIVGFETGDVLPAGSRGIVEVRGPQVMKGYYK 256
              N +G ++  +      + G E G        G +++RGP +M+GY +
Sbjct: 536 ---NTVGRILPGMESRVIPVPGIEQG--------GRLQLRGPNIMRGYLR 569

[Close]

BLAST of MC07g_new0302 vs. ExPASy Swiss-Prot
Match: P31686 (4-coumarate--CoA ligase 1 (Fragment) OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.9e-04
Identity = 27/71 (38.03%), Postives = 37/71 (52.11%), Query Frame = 0


Query: 183 GYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVLPAGSRGIV 242
           GYG+TE  P++ +S   +   +       G VV   R+ E  IV  ETG  LP    G +
Sbjct: 82  GYGMTEAGPVLTMSLAFAKEPIDVKPGACGTVV---RNAEMKIVDPETGHSLPRNQSGEI 141

Query: 243 EVRGPQVMKGY 254
            +RG Q+MKGY
Sbjct: 142 CIRGDQIMKGY 149

[Close]

BLAST of MC07g_new0302 vs. NCBI nr
Match: XP_022147167.1 (probable acyl-activating enzyme 16, chloroplastic [Momordica charantia])

HSP 1 Score: 121 bits (304), Expect = 3.02e-26
Identity = 63/81 (77.78%), Postives = 64/81 (79.01%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV FETGDVL
Sbjct: 482 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDFETGDVL 541

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           PAGSRGIVEVRGPQVMKGYYK
Sbjct: 542 PAGSRGIVEVRGPQVMKGYYK 554

[Close]

BLAST of MC07g_new0302 vs. NCBI nr
Match: XP_038874366.1 (probable acyl-activating enzyme 16, chloroplastic [Benincasa hispida])

HSP 1 Score: 117 bits (293), Expect = 8.37e-25
Identity = 61/81 (75.31%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 481 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 540

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEVRGPQVMKGYYK
Sbjct: 541 PPGSRGIVEVRGPQVMKGYYK 553

[Close]

BLAST of MC07g_new0302 vs. NCBI nr
Match: XP_023548747.1 (probable acyl-activating enzyme 16, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 116 bits (290), Expect = 1.65e-24
Identity = 60/81 (74.07%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 363 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 422

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEV+GPQVMKGYYK
Sbjct: 423 PPGSRGIVEVKGPQVMKGYYK 435

[Close]

BLAST of MC07g_new0302 vs. NCBI nr
Match: XP_023548746.1 (probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 116 bits (290), Expect = 1.94e-24
Identity = 60/81 (74.07%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 433 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 492

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEV+GPQVMKGYYK
Sbjct: 493 PPGSRGIVEVKGPQVMKGYYK 505

[Close]

BLAST of MC07g_new0302 vs. NCBI nr
Match: XP_022958899.1 (probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 116 bits (290), Expect = 1.94e-24
Identity = 60/81 (74.07%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 433 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 492

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEV+GPQVMKGYYK
Sbjct: 493 PPGSRGIVEVKGPQVMKGYYK 505

[Close]

BLAST of MC07g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1D1M2 (probable acyl-activating enzyme 16, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016178 PE=4 SV=1)

HSP 1 Score: 121 bits (304), Expect = 1.46e-26
Identity = 63/81 (77.78%), Postives = 64/81 (79.01%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV FETGDVL
Sbjct: 482 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDFETGDVL 541

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           PAGSRGIVEVRGPQVMKGYYK
Sbjct: 542 PAGSRGIVEVRGPQVMKGYYK 554

[Close]

BLAST of MC07g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1L552 (probable acyl-activating enzyme 16, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499223 PE=4 SV=1)

HSP 1 Score: 116 bits (290), Expect = 9.40e-25
Identity = 60/81 (74.07%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 433 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 492

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEV+GPQVMKGYYK
Sbjct: 493 PPGSRGIVEVKGPQVMKGYYK 505

[Close]

BLAST of MC07g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1H6F8 (probable acyl-activating enzyme 16, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460048 PE=4 SV=1)

HSP 1 Score: 116 bits (290), Expect = 9.40e-25
Identity = 60/81 (74.07%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 433 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 492

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEV+GPQVMKGYYK
Sbjct: 493 PPGSRGIVEVKGPQVMKGYYK 505

[Close]

BLAST of MC07g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1KW26 (probable acyl-activating enzyme 16, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499223 PE=4 SV=1)

HSP 1 Score: 116 bits (290), Expect = 1.00e-24
Identity = 60/81 (74.07%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 484 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 543

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEV+GPQVMKGYYK
Sbjct: 544 PPGSRGIVEVKGPQVMKGYYK 556

[Close]

BLAST of MC07g_new0302 vs. ExPASy TrEMBL
Match: A0A6J1H4D5 (probable acyl-activating enzyme 16, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460048 PE=4 SV=1)

HSP 1 Score: 116 bits (290), Expect = 1.00e-24
Identity = 60/81 (74.07%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 175 AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVL 234
           AIGITVQNGYGLTECSP+IA             CNVLG V HPIRHTEF IV  ETGDVL
Sbjct: 484 AIGITVQNGYGLTECSPVIAARRPT--------CNVLGSVGHPIRHTEFRIVDMETGDVL 543

Query: 235 PAGSRGIVEVRGPQVMKGYYK 255
           P GSRGIVEV+GPQVMKGYYK
Sbjct: 544 PPGSRGIVEVKGPQVMKGYYK 556

[Close]

BLAST of MC07g_new0302 vs. TAIR 10
Match: AT3G23790.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.4e-20
Identity = 58/95 (61.05%), Postives = 64/95 (67.37%), Query Frame = 0


Query: 166 GFGCSPIQV-----AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRH 225
           G G  P+ V     AIG+ VQNGYGLTE SP+      VSA  L   CNVLG V HPI+ 
Sbjct: 457 GGGSLPMHVDKFFEAIGVNVQNGYGLTETSPV------VSARRLR--CNVLGSVGHPIKD 516

Query: 226 TEFGIVGFETGDVLPAGSRGIVEVRGPQVMKGYYK 256
           TEF IV  ETG VLP GS+GIV+VRGP VMKGYYK
Sbjct: 517 TEFKIVDHETGTVLPPGSKGIVKVRGPPVMKGYYK 543

[Close]

BLAST of MC07g_new0302 vs. TAIR 10
Match: AT4G14070.1 (acyl-activating enzyme 15 )

HSP 1 Score: 94.0 bits (232), Expect = 2.6e-19
Identity = 53/95 (55.79%), Postives = 64/95 (67.37%), Query Frame = 0


Query: 166 GFGCSPIQV-----AIGITVQNGYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRH 225
           G G  PI V     AIG+ +QNGYGLTE SP++  + T+S       CNVLG   HP+  
Sbjct: 473 GGGSLPIHVDKFFEAIGVILQNGYGLTETSPVVC-ARTLS-------CNVLGSAGHPMHG 532

Query: 226 TEFGIVGFETGDVLPAGSRGIVEVRGPQVMKGYYK 256
           TEF IV  ET +VLP GS+GI++VRGPQVMKGYYK
Sbjct: 533 TEFKIVDPETNNVLPPGSKGIIKVRGPQVMKGYYK 559

[Close]

BLAST of MC07g_new0302 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 47.4 bits (111), Expect = 2.8e-05
Identity = 27/71 (38.03%), Postives = 38/71 (53.52%), Query Frame = 0


Query: 183 GYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVLPAGSRGIV 242
           GYG+TE  P++A+S   +           G VV   R+ E  I+  +TGD LP    G +
Sbjct: 346 GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV---RNAEMKILDPDTGDSLPRNKPGEI 405

Query: 243 EVRGPQVMKGY 254
            +RG Q+MKGY
Sbjct: 406 CIRGNQIMKGY 413

[Close]

BLAST of MC07g_new0302 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 45.4 bits (106), Expect = 1.1e-04
Identity = 27/71 (38.03%), Postives = 37/71 (52.11%), Query Frame = 0


Query: 183 GYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVLPAGSRGIV 242
           GYG+TE  P++A+S   +           G VV   R+ E  IV  +TGD L     G +
Sbjct: 353 GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV---RNAEMKIVDPDTGDSLSRNQPGEI 412

Query: 243 EVRGPQVMKGY 254
            +RG Q+MKGY
Sbjct: 413 CIRGHQIMKGY 420

[Close]

BLAST of MC07g_new0302 vs. TAIR 10
Match: AT1G51680.2 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 45.4 bits (106), Expect = 1.1e-04
Identity = 27/71 (38.03%), Postives = 37/71 (52.11%), Query Frame = 0


Query: 183 GYGLTECSPLIALSYTVSAILLSNGCNVLGLVVHPIRHTEFGIVGFETGDVLPAGSRGIV 242
           GYG+TE  P++A+S   +           G VV   R+ E  IV  +TGD L     G +
Sbjct: 353 GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV---RNAEMKIVDPDTGDSLSRNQPGEI 412

Query: 243 EVRGPQVMKGY 254
            +RG Q+MKGY
Sbjct: 413 CIRGHQIMKGY 420

The following BLAST results are available for this feature:

BLAST of MC07g_new0302 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LK39	1.9e-19	61.05	Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q8W471	3.6e-18	55.79	Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Ara...	[more]
P41636	3.0e-04	35.21	4-coumarate--CoA ligase OS=Pinus taeda OX=3352 GN=4CL PE=2 SV=1	[more]
A1JPF0	3.0e-04	27.27	Bifunctional protein Aas OS=Yersinia enterocolitica serotype O:8 / biotype 1B (s...	[more]
P31686	3.9e-04	38.03	4-coumarate--CoA ligase 1 (Fragment) OS=Glycine max OX=3847 PE=2 SV=1	[more]

BLAST of MC07g_new0302 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147167.1	3.02e-26	77.78	probable acyl-activating enzyme 16, chloroplastic [Momordica charantia]	[more]
XP_038874366.1	8.37e-25	75.31	probable acyl-activating enzyme 16, chloroplastic [Benincasa hispida]	[more]
XP_023548747.1	1.65e-24	74.07	probable acyl-activating enzyme 16, chloroplastic isoform X3 [Cucurbita pepo sub...	[more]
XP_023548746.1	1.94e-24	74.07	probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita pepo sub...	[more]
XP_022958899.1	1.94e-24	74.07	probable acyl-activating enzyme 16, chloroplastic isoform X2 [Cucurbita moschata...	[more]

BLAST of MC07g_new0302 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D1M2	1.46e-26	77.78	probable acyl-activating enzyme 16, chloroplastic OS=Momordica charantia OX=3673...	[more]
A0A6J1L552	9.40e-25	74.07	probable acyl-activating enzyme 16, chloroplastic isoform X2 OS=Cucurbita maxima...	[more]
A0A6J1H6F8	9.40e-25	74.07	probable acyl-activating enzyme 16, chloroplastic isoform X2 OS=Cucurbita moscha...	[more]
A0A6J1KW26	1.00e-24	74.07	probable acyl-activating enzyme 16, chloroplastic isoform X1 OS=Cucurbita maxima...	[more]
A0A6J1H4D5	1.00e-24	74.07	probable acyl-activating enzyme 16, chloroplastic isoform X1 OS=Cucurbita moscha...	[more]

BLAST of MC07g_new0302 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23790.1	1.4e-20	61.05	AMP-dependent synthetase and ligase family protein	[more]
AT4G14070.1	2.6e-19	55.79	acyl-activating enzyme 15	[more]
AT3G21240.1	2.8e-05	38.03	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	1.1e-04	38.03	4-coumarate:CoA ligase 1	[more]
AT1G51680.2	1.1e-04	38.03	4-coumarate:CoA ligase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 82..109
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 281..302
None	No IPR available	PANTHER	PTHR43813:SF4	LONG-CHAIN-FATTY-ACID COA LIGASE (AMP-FORMING)	coord: 174..256
None	No IPR available	PANTHER	PTHR43813	ACYL-ACTIVATING ENZYME 16, CHLOROPLASTIC-RELATED	coord: 174..256
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 174..255
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 147..255 e-value: 2.3E-13 score: 49.6
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 122..256 e-value: 6.7E-14 score: 53.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g_new0302.1	MC07g_new0302.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane