MC07g_new0060 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g_new0060
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRibosomal L18p/L5e family protein
LocationMC07: 2096337 .. 2096717 (-)
RNA-Seq ExpressionMC07g_new0060
SyntenyMC07g_new0060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGAAGCCGGAGGCACCTCCTCCAAGTGGTTGTTTCCTGCCGGAAAATCAGCGCTCAGGTCACAAATCCGGCGAGCTCCTCCATCGTCGCCATGGCCTCATCCTCGGAGCAGGAATTCGTGGCCTACAACCGATCGAAACTCAACCGCTTCCCCCGATCCAACAACTTCTGGGACTCCATGATCGCCTCCCGCGTCGGCGAAAAGCTAGGGCTCCGCCTGACCGAGATCGGCGTCTCCGATGTTCAGATCGACCTCCGGGAAGAGCTCTCGCGCCCTGCTTACTACAGGAAGATGGTGCTGCCGCTGTTCGATTCCGTGCGCCGCTCCGGCGTCGCCATTGACGGAGCTGAGAACCTTGGAACAGGTGCGATCTAA

mRNA sequence

ATGTCGAGAAGCCGGAGGCACCTCCTCCAAGTGGTTGTTTCCTGCCGGAAAATCAGCGCTCAGGTCACAAATCCGGCGAGCTCCTCCATCGTCGCCATGGCCTCATCCTCGGAGCAGGAATTCGTGGCCTACAACCGATCGAAACTCAACCGCTTCCCCCGATCCAACAACTTCTGGGACTCCATGATCGCCTCCCGCGTCGGCGAAAAGCTAGGGCTCCGCCTGACCGAGATCGGCGTCTCCGATGTTCAGATCGACCTCCGGGAAGAGCTCTCGCGCCCTGCTTACTACAGGAAGATGGTGCTGCCGCTGTTCGATTCCGTGCGCCGCTCCGGCGTCGCCATTGACGGAGCTGAGAACCTTGGAACAGGTGCGATCTAA

Coding sequence (CDS)

ATGTCGAGAAGCCGGAGGCACCTCCTCCAAGTGGTTGTTTCCTGCCGGAAAATCAGCGCTCAGGTCACAAATCCGGCGAGCTCCTCCATCGTCGCCATGGCCTCATCCTCGGAGCAGGAATTCGTGGCCTACAACCGATCGAAACTCAACCGCTTCCCCCGATCCAACAACTTCTGGGACTCCATGATCGCCTCCCGCGTCGGCGAAAAGCTAGGGCTCCGCCTGACCGAGATCGGCGTCTCCGATGTTCAGATCGACCTCCGGGAAGAGCTCTCGCGCCCTGCTTACTACAGGAAGATGGTGCTGCCGCTGTTCGATTCCGTGCGCCGCTCCGGCGTCGCCATTGACGGAGCTGAGAACCTTGGAACAGGTGCGATCTAA

Protein sequence

MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWDSMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAENLGTGAI
Homology
BLAST of MC07g_new0060 vs. NCBI nr
Match: XP_022150463.1 (uncharacterized protein LOC111018600 [Momordica charantia])

HSP 1 Score: 237 bits (604), Expect = 2.53e-78
Identity = 124/126 (98.41%), Postives = 125/126 (99.21%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD
Sbjct: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN
Sbjct: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120

Query: 121 LGTGAI 126
           LGTG +
Sbjct: 121 LGTGFV 126

BLAST of MC07g_new0060 vs. NCBI nr
Match: XP_004140885.1 (uncharacterized protein LOC101204363 [Cucumis sativus])

HSP 1 Score: 201 bits (512), Expect = 2.36e-64
Identity = 103/126 (81.75%), Postives = 117/126 (92.86%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS ++ HLL++V+SCRKI+AQVTNPA+SSI+AMASSSEQEFVAY RSKL+RFPRSNNFWD
Sbjct: 1   MSINQNHLLRLVLSCRKITAQVTNPATSSIIAMASSSEQEFVAYYRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S +ASRVGEKLG RL EIGVSDV+IDL EELSRP YYRKMVLPLFDSV+RSGVA+DGAE 
Sbjct: 61  SKVASRVGEKLGHRLKEIGVSDVRIDLAEELSRPIYYRKMVLPLFDSVQRSGVAVDGAEK 120

Query: 121 LGTGAI 126
           LGTG+I
Sbjct: 121 LGTGSI 126

BLAST of MC07g_new0060 vs. NCBI nr
Match: XP_038884099.1 (uncharacterized protein LOC120075027 isoform X6 [Benincasa hispida])

HSP 1 Score: 199 bits (507), Expect = 1.50e-63
Identity = 103/124 (83.06%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS S+ HLL++V+SCRKI+AQVTNPA+SSIVAMASSSEQEFVAY RSKL+RFPRSNNFWD
Sbjct: 1   MSISQNHLLRLVLSCRKITAQVTNPATSSIVAMASSSEQEFVAYYRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S +ASRVGEKLG RL EIGVSDV+IDL EE SRP YYRKMVLPLFDSV+RSGVA+DGAE 
Sbjct: 61  SKVASRVGEKLGHRLKEIGVSDVRIDLAEEFSRPLYYRKMVLPLFDSVQRSGVAVDGAEK 120

Query: 121 LGTG 124
           LGTG
Sbjct: 121 LGTG 124

BLAST of MC07g_new0060 vs. NCBI nr
Match: XP_038884098.1 (uncharacterized protein LOC120075027 isoform X5 [Benincasa hispida])

HSP 1 Score: 199 bits (507), Expect = 1.55e-63
Identity = 103/124 (83.06%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS S+ HLL++V+SCRKI+AQVTNPA+SSIVAMASSSEQEFVAY RSKL+RFPRSNNFWD
Sbjct: 1   MSISQNHLLRLVLSCRKITAQVTNPATSSIVAMASSSEQEFVAYYRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S +ASRVGEKLG RL EIGVSDV+IDL EE SRP YYRKMVLPLFDSV+RSGVA+DGAE 
Sbjct: 61  SKVASRVGEKLGHRLKEIGVSDVRIDLAEEFSRPLYYRKMVLPLFDSVQRSGVAVDGAEK 120

Query: 121 LGTG 124
           LGTG
Sbjct: 121 LGTG 124

BLAST of MC07g_new0060 vs. NCBI nr
Match: XP_038884096.1 (uncharacterized protein LOC120075027 isoform X3 [Benincasa hispida])

HSP 1 Score: 199 bits (507), Expect = 1.77e-63
Identity = 103/124 (83.06%), Postives = 114/124 (91.94%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS S+ HLL++V+SCRKI+AQVTNPA+SSIVAMASSSEQEFVAY RSKL+RFPRSNNFWD
Sbjct: 1   MSISQNHLLRLVLSCRKITAQVTNPATSSIVAMASSSEQEFVAYYRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S +ASRVGEKLG RL EIGVSDV+IDL EE SRP YYRKMVLPLFDSV+RSGVA+DGAE 
Sbjct: 61  SKVASRVGEKLGHRLKEIGVSDVRIDLAEEFSRPLYYRKMVLPLFDSVQRSGVAVDGAEK 120

Query: 121 LGTG 124
           LGTG
Sbjct: 121 LGTG 124

BLAST of MC07g_new0060 vs. ExPASy TrEMBL
Match: A0A6J1D9I9 (uncharacterized protein LOC111018600 OS=Momordica charantia OX=3673 GN=LOC111018600 PE=4 SV=1)

HSP 1 Score: 237 bits (604), Expect = 1.22e-78
Identity = 124/126 (98.41%), Postives = 125/126 (99.21%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD
Sbjct: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN
Sbjct: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120

Query: 121 LGTGAI 126
           LGTG +
Sbjct: 121 LGTGFV 126

BLAST of MC07g_new0060 vs. ExPASy TrEMBL
Match: A0A0A0KE39 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G042360 PE=4 SV=1)

HSP 1 Score: 201 bits (512), Expect = 1.14e-64
Identity = 103/126 (81.75%), Postives = 117/126 (92.86%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS ++ HLL++V+SCRKI+AQVTNPA+SSI+AMASSSEQEFVAY RSKL+RFPRSNNFWD
Sbjct: 1   MSINQNHLLRLVLSCRKITAQVTNPATSSIIAMASSSEQEFVAYYRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S +ASRVGEKLG RL EIGVSDV+IDL EELSRP YYRKMVLPLFDSV+RSGVA+DGAE 
Sbjct: 61  SKVASRVGEKLGHRLKEIGVSDVRIDLAEELSRPIYYRKMVLPLFDSVQRSGVAVDGAEK 120

Query: 121 LGTGAI 126
           LGTG+I
Sbjct: 121 LGTGSI 126

BLAST of MC07g_new0060 vs. ExPASy TrEMBL
Match: A0A5D3BWW4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold121G00630 PE=4 SV=1)

HSP 1 Score: 194 bits (493), Expect = 8.99e-62
Identity = 99/126 (78.57%), Postives = 114/126 (90.48%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS ++ HLL++V+SCRKI+AQVTNPA+SSI+AMASSSEQEFVAY RSKL+RFPRSNNFWD
Sbjct: 1   MSINQNHLLRLVLSCRKITAQVTNPATSSIIAMASSSEQEFVAYYRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S +ASRVG KLG RL EIGVSDV+IDL EELSRP YYRKMVLPLFDSV+RSG+A+DG E 
Sbjct: 61  SKVASRVGIKLGHRLKEIGVSDVRIDLAEELSRPIYYRKMVLPLFDSVQRSGIAVDGVEK 120

Query: 121 LGTGAI 126
           LG G+I
Sbjct: 121 LGKGSI 126

BLAST of MC07g_new0060 vs. ExPASy TrEMBL
Match: A0A1S4DVU4 (uncharacterized protein LOC103488672 OS=Cucumis melo OX=3656 GN=LOC103488672 PE=4 SV=1)

HSP 1 Score: 194 bits (493), Expect = 8.99e-62
Identity = 99/126 (78.57%), Postives = 114/126 (90.48%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS ++ HLL++V+SCRKI+AQVTNPA+SSI+AMASSSEQEFVAY RSKL+RFPRSNNFWD
Sbjct: 1   MSINQNHLLRLVLSCRKITAQVTNPATSSIIAMASSSEQEFVAYYRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S +ASRVG KLG RL EIGVSDV+IDL EELSRP YYRKMVLPLFDSV+RSG+A+DG E 
Sbjct: 61  SKVASRVGIKLGHRLKEIGVSDVRIDLAEELSRPIYYRKMVLPLFDSVQRSGIAVDGVEK 120

Query: 121 LGTGAI 126
           LG G+I
Sbjct: 121 LGKGSI 126

BLAST of MC07g_new0060 vs. ExPASy TrEMBL
Match: A0A6J1EVG7 (uncharacterized protein LOC111436340 OS=Cucurbita moschata OX=3662 GN=LOC111436340 PE=4 SV=1)

HSP 1 Score: 193 bits (491), Expect = 1.81e-61
Identity = 100/126 (79.37%), Postives = 114/126 (90.48%), Query Frame = 0

Query: 1   MSRSRRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWD 60
           MS ++ HLL++V+SCRKI+AQVTNP +S+I+AMASSSEQEFVAYNRSKL+RFPRSNNFWD
Sbjct: 1   MSINQNHLLRLVLSCRKITAQVTNPDTSTIIAMASSSEQEFVAYNRSKLHRFPRSNNFWD 60

Query: 61  SMIASRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAEN 120
           S IASRVGEKLG RL EIGVSDV+IDL EELSRP  YR  VLPLFDSVRRSGVA++GAE 
Sbjct: 61  SKIASRVGEKLGHRLKEIGVSDVRIDLAEELSRPVSYRNRVLPLFDSVRRSGVAVNGAEK 120

Query: 121 LGTGAI 126
           LG+GAI
Sbjct: 121 LGSGAI 126

BLAST of MC07g_new0060 vs. TAIR 10
Match: AT2G43310.1 (Ribosomal L18p/L5e family protein )

HSP 1 Score: 162.9 bits (411), Expect = 1.6e-40
Identity = 81/117 (69.23%), Postives = 97/117 (82.91%), Query Frame = 0

Query: 5   RRHLLQVVVSCRKISAQVTNPASSSIVAMASSSEQEFVAYNRSKLNRFPRSNNFWDSMIA 64
           R HLLQ+V+SCRKI+AQVT P SS+I+AMASSSEQEF+A +R+ L RFP SN+FWDS  A
Sbjct: 6   RHHLLQLVLSCRKITAQVTQPGSSTIIAMASSSEQEFLAKSRANLYRFPHSNHFWDSKTA 65

Query: 65  SRVGEKLGLRLTEIGVSDVQIDLREELSRPAYYRKMVLPLFDSVRRSGVAIDGAENL 122
           SRVGEKLGLRL E+GV  V ID  EE+SRP ++RK VLPLFDSVRR+G+ +DG E L
Sbjct: 66  SRVGEKLGLRLRELGVDVVSIDADEEISRPIHHRKRVLPLFDSVRRTGIRVDGTEQL 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022150463.12.53e-7898.41uncharacterized protein LOC111018600 [Momordica charantia][more]
XP_004140885.12.36e-6481.75uncharacterized protein LOC101204363 [Cucumis sativus][more]
XP_038884099.11.50e-6383.06uncharacterized protein LOC120075027 isoform X6 [Benincasa hispida][more]
XP_038884098.11.55e-6383.06uncharacterized protein LOC120075027 isoform X5 [Benincasa hispida][more]
XP_038884096.11.77e-6383.06uncharacterized protein LOC120075027 isoform X3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1D9I91.22e-7898.41uncharacterized protein LOC111018600 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A0A0KE391.14e-6481.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G042360 PE=4 SV=1[more]
A0A5D3BWW48.99e-6278.57Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S4DVU48.99e-6278.57uncharacterized protein LOC103488672 OS=Cucumis melo OX=3656 GN=LOC103488672 PE=... [more]
A0A6J1EVG71.81e-6179.37uncharacterized protein LOC111436340 OS=Cucurbita moschata OX=3662 GN=LOC1114363... [more]
Match NameE-valueIdentityDescription
AT2G43310.11.6e-4069.23Ribosomal L18p/L5e family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.420.100coord: 1..114
e-value: 3.4E-9
score: 39.1
NoneNo IPR availablePANTHERPTHR12899:SF14F14B2.25/F14B2.25coord: 3..123
NoneNo IPR availableSUPERFAMILY53137Translational machinery componentscoord: 3..112
IPR005484Ribosomal protein L18PANTHERPTHR1289939S RIBOSOMAL PROTEIN L18, MITOCHONDRIALcoord: 3..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g_new0060.1MC07g_new0060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005840 ribosome
molecular_function GO:0008097 5S rRNA binding
molecular_function GO:0003735 structural constituent of ribosome