MC07g_new0052 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g_new0052
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionnatterin-2-like
LocationMC07: 1885708 .. 1887128 (-)
RNA-Seq ExpressionMC07g_new0052
SyntenyMC07g_new0052
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGCACCTTCCAAGGTACCATTCAAATCTAAATTAAAGAACGACTACATAAAAATCGGCTATTTGCGGTATGTGAACGAAGATTCAGAAATAAAAACATATTTGCAATACTCTGGAGAGGCCGTTCTAAGTCCATACACAAAGTTTGAAGTAGAACGGGCTAAATGTGACCCCTCATTGGTCAATATACGATGTTGTTACAACAACAAATATTGGGTTAGTTTGGCTGAAACTAAATATTCTTATTTTACTGCTGCAAGGGCTGAAAAGCCCGAGGAAAATTAATCTAAATGGAATTGCACACTGTTCCAACCTGTTTATGATAGTTCTCATCAAGCATTTCGATTTCGCCATGTTTTTTCTAATTATTTTGTGACCTTGTGCCGAGATGGTTTTGCTCAAGGGAAATGCTTGTATGCAAATCTTCCATTCCCTAACGATTGGAATCTTGGTGATCTTAACACAATCATTGATTATGAAGCCTTGGTTTCAATACCTAAGTATGTTGCTTTCAAGGGTCATAATGGTTGCTACCTCAGTTCTCGTTGGATTGAAGGTCATCAATATCACCAATTTTCATCAAGTGATATTGGCGATTCTAGGGTTCGAATGGAAACGTTCATCACAAAAGATGGAAATATTAGAATTAAGTCAAATTACTTTGGAAAATTTTGGAGACGTAGTCCTAATTGGATTTGGGCTGATTCGACTGACACCACAACCGACGATCCTGATACTTTGTTTTGCCCAACTAAAATTGATAACCATGTGATCGCTCTTCGTAACTTGGGTAATAAAAACTTTATCAAGAGGTTCACATATGAATACAAGACAAGTTGTCTTAATGCTGCTACGGACTCAATTGATGCATATTCCCGATTACACATGGAAGAGCCTATTCTTGAAAGAGAAATTTATGATATAAATTTCCATCCTGCCGATTCAAGAATCTATGATCAAACTGTACTTGTTATGGCCACTGGGAATGGTACCAACCGAACAGAAGTACCCAACACAATGAACTTGAATCTATCGTATATTGAAACCGAATCAAGCACGTGGAGTTCTTCGATCTCTATTAAATTGGGTGTTAAAACAAGTATTGAAACAGGAATTCCTCGTATTGTTGATGGAAAGGTTGAAGTATCGTCTGAGTTTACTGGATCATACCAATGGGGAAAAACTATAACAAAAACGCAGACAATTGGAAGCACATATCAAATAACTGTGCCACCAAAAACTACTGTGATTGTCAGTTTGTTGGCAACTACAGGTAAATGTGATGTTCCCTACTCGTATAGTCAACGTGACACTCTCATTAATGGGGAAATAAAATATTATGAAATGGATGATGGTGTATACCACGGTGTCAATAGCTACAATTTCACATATGAAACGAAAAGCAAACCAGCAAGTTAA

mRNA sequence

ATGGTGCACCTTCCAAGTTCTCATCAAGCATTTCGATTTCGCCATGTTTTTTCTAATTATTTTGTGACCTTGTGCCGAGATGGTTTTGCTCAAGGGAAATGCTTGTATGCAAATCTTCCATTCCCTAACGATTGGAATCTTGGTGATCTTAACACAATCATTGATTATGAAGCCTTGGTTTCAATACCTAAGTATGTTGCTTTCAAGGGTCATAATGGTTGCTACCTCAGTTCTCGTTGGATTGAAGGTCATCAATATCACCAATTTTCATCAAGTGATATTGGCGATTCTAGGGTTCGAATGGAAACGTTCATCACAAAAGATGGAAATATTAGAATTAAGTCAAATTACTTTGGAAAATTTTGGAGACGTAGTCCTAATTGGATTTGGGCTGATTCGACTGACACCACAACCGACGATCCTGATACTTTGTTTTGCCCAACTAAAATTGATAACCATGTGATCGCTCTTCGTAACTTGGGTAATAAAAACTTTATCAAGAGGTTCACATATGAATACAAGACAAGTTGTCTTAATGCTGCTACGGACTCAATTGATGCATATTCCCGATTACACATGGAAGAGCCTATTCTTGAAAGAGAAATTTATGATATAAATTTCCATCCTGCCGATTCAAGAATCTATGATCAAACTGTACTTGTTATGGCCACTGGGAATGGTACCAACCGAACAGAAGTACCCAACACAATGAACTTGAATCTATCGTATATTGAAACCGAATCAAGCACGTGGAGTTCTTCGATCTCTATTAAATTGGGTGTTAAAACAAGTATTGAAACAGGAATTCCTCGTATTGTTGATGGAAAGGTTGAAGTATCGTCTGAGTTTACTGGATCATACCAATGGGGAAAAACTATAACAAAAACGCAGACAATTGGAAGCACATATCAAATAACTGTGCCACCAAAAACTACTGTGATTGTCAGTTTGTTGGCAACTACAGGTAAATGTGATGTTCCCTACTCGTATAGTCAACGTGACACTCTCATTAATGGGGAAATAAAATATTATGAAATGGATGATGGTGTATACCACGGTGTCAATAGCTACAATTTCACATATGAAACGAAAAGCAAACCAGCAAGTTAA

Coding sequence (CDS)

ATGGTGCACCTTCCAAGTTCTCATCAAGCATTTCGATTTCGCCATGTTTTTTCTAATTATTTTGTGACCTTGTGCCGAGATGGTTTTGCTCAAGGGAAATGCTTGTATGCAAATCTTCCATTCCCTAACGATTGGAATCTTGGTGATCTTAACACAATCATTGATTATGAAGCCTTGGTTTCAATACCTAAGTATGTTGCTTTCAAGGGTCATAATGGTTGCTACCTCAGTTCTCGTTGGATTGAAGGTCATCAATATCACCAATTTTCATCAAGTGATATTGGCGATTCTAGGGTTCGAATGGAAACGTTCATCACAAAAGATGGAAATATTAGAATTAAGTCAAATTACTTTGGAAAATTTTGGAGACGTAGTCCTAATTGGATTTGGGCTGATTCGACTGACACCACAACCGACGATCCTGATACTTTGTTTTGCCCAACTAAAATTGATAACCATGTGATCGCTCTTCGTAACTTGGGTAATAAAAACTTTATCAAGAGGTTCACATATGAATACAAGACAAGTTGTCTTAATGCTGCTACGGACTCAATTGATGCATATTCCCGATTACACATGGAAGAGCCTATTCTTGAAAGAGAAATTTATGATATAAATTTCCATCCTGCCGATTCAAGAATCTATGATCAAACTGTACTTGTTATGGCCACTGGGAATGGTACCAACCGAACAGAAGTACCCAACACAATGAACTTGAATCTATCGTATATTGAAACCGAATCAAGCACGTGGAGTTCTTCGATCTCTATTAAATTGGGTGTTAAAACAAGTATTGAAACAGGAATTCCTCGTATTGTTGATGGAAAGGTTGAAGTATCGTCTGAGTTTACTGGATCATACCAATGGGGAAAAACTATAACAAAAACGCAGACAATTGGAAGCACATATCAAATAACTGTGCCACCAAAAACTACTGTGATTGTCAGTTTGTTGGCAACTACAGGTAAATGTGATGTTCCCTACTCGTATAGTCAACGTGACACTCTCATTAATGGGGAAATAAAATATTATGAAATGGATGATGGTGTATACCACGGTGTCAATAGCTACAATTTCACATATGAAACGAAAAGCAAACCAGCAAGTTAA

Protein sequence

MVHLPSSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKYVAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRSPNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSCLNAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETKSKPAS
Homology
BLAST of MC07g_new0052 vs. ExPASy Swiss-Prot
Match: Q66S21 (Natterin-2 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.6e-09
Identity = 54/204 (26.47%), Postives = 92/204 (45.10%), Query Frame = 0

Query: 162 NKNFIKRFTY-----EYKTSCLNAATDSID----AYSRLHMEEPILEREIYDINFHPADS 221
           NK ++ +  Y     E    CL    D  +     Y  L M + ++E+ + D+ +     
Sbjct: 130 NKIYVGKGAYGLGKIEPANHCLYYVWDGAETWTKTYQALTMNKDVIEQAMKDVKYQTEGV 189

Query: 222 RIYDQTVLVM--ATGNGTNRTEVPNTMNLNLSYIETESSTWSSSISIKLGVKTSIETGIP 281
            +      VM  +T N  +  EV  T+ L    I T+   W  + S+  GV T++  GIP
Sbjct: 190 TVIKGKPEVMRRSTVNNQHCKEVTKTVTLTKD-ISTD-ERWDVTNSVTFGVTTTVTAGIP 249

Query: 282 RIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQITVPPKTTVIVSLLATTGKCDVPYSY 341
            +    +E+S + T  +  G + T+TQ+   T  + VPPK +  VS++A   K D+P++ 
Sbjct: 250 DVSSASLEISMQATMDFAHGASKTETQSYMVTVSVPVPPKQSCTVSMVAQVNKADIPFTA 309

Query: 342 SQRDTLINGEIKYYEMDDGVYHGV 355
           +   T   G+ K      GVY  +
Sbjct: 310 TLIRTYRGGK-KTQTTTKGVYRTI 330

BLAST of MC07g_new0052 vs. ExPASy Swiss-Prot
Match: Q66S25 (Natterin-1 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.6e-08
Identity = 49/175 (28.00%), Postives = 80/175 (45.71%), Query Frame = 0

Query: 179 NAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVM--ATGNGTNRTEVPNT 238
           N A      Y  L + + ++E+ + D+ +      +      VM  +T N     EV  T
Sbjct: 155 NGAETWTKTYQALTVNKDVIEQTMKDVKYQTEGVTVIQGKPEVMRKSTVNNKQCKEVTKT 214

Query: 239 MNLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKT 298
           + L+   I TE   W  + S+  GV T++  GIP +    + VS E    +  G + T++
Sbjct: 215 VTLSKD-ISTE-ERWDVTNSVTFGVTTTVTAGIPDVASASLAVSMEARRDFAHGASKTES 274

Query: 299 QTIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVY 352
           Q+   T  + VPPK +  VS++A   K DVP++ +   T   G+ K      GVY
Sbjct: 275 QSYMVTVSVPVPPKQSCTVSMVAQVNKADVPFTATLIRTYRGGK-KTQTTTKGVY 326

BLAST of MC07g_new0052 vs. NCBI nr
Match: XP_022150377.1 (uncharacterized protein LOC111018553 [Momordica charantia])

HSP 1 Score: 667 bits (1722), Expect = 4.88e-239
Identity = 325/365 (89.04%), Postives = 347/365 (95.07%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           SSHQAFRFRHVF+ YFV L + GFA+G CL A+ P+P+D +LGDLNT+IDYEALVSIPKY
Sbjct: 111 SSHQAFRFRHVFTGYFVALWQVGFAEGNCLRASFPYPDDCSLGDLNTVIDYEALVSIPKY 170

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           VAFKG N CYLSSRWIEGH+YHQFSSSD+GDSRVRMETFITKDGNIRIKSNYFGKFWRRS
Sbjct: 171 VAFKGDNDCYLSSRWIEGHEYHQFSSSDLGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 230

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNH-VIALRNLGNKNFIKRFTYEYKTSCLNAATDS 185
           PNWIWADSTDT+T++PDTLFCPTK+DN+ VIALRNLGN NFIKR T E KTSCLNAATDS
Sbjct: 231 PNWIWADSTDTSTNNPDTLFCPTKVDNNNVIALRNLGNNNFIKRLTTEGKTSCLNAATDS 290

Query: 186 IDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI 245
           IDAYSRL MEEP+LEREIY+INFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI
Sbjct: 291 IDAYSRLQMEEPVLEREIYNINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI 350

Query: 246 ETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQ 305
           ETESS WSSSISIKLGVKT+I+TGIPRIVDGKVEVSSE TGSYQWGKTITKTQT+GSTYQ
Sbjct: 351 ETESSAWSSSISIKLGVKTTIQTGIPRIVDGKVEVSSELTGSYQWGKTITKTQTVGSTYQ 410

Query: 306 ITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETK 365
           +TVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVN+YNFTYETK
Sbjct: 411 VTVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNNYNFTYETK 470

Query: 366 SKPAS 369
           SKPAS
Sbjct: 471 SKPAS 475

BLAST of MC07g_new0052 vs. NCBI nr
Match: XP_022150378.1 (natterin-2-like [Momordica charantia])

HSP 1 Score: 634 bits (1636), Expect = 1.99e-228
Identity = 311/312 (99.68%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 58  ALVSIPKYVAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNY 117
           +LVSIPKYVAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNY
Sbjct: 9   SLVSIPKYVAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNY 68

Query: 118 FGKFWRRSPNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSC 177
           FGKFWRRSPNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSC
Sbjct: 69  FGKFWRRSPNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSC 128

Query: 178 LNAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTM 237
           LNAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTM
Sbjct: 129 LNAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTM 188

Query: 238 NLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQ 297
           NLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQ
Sbjct: 189 NLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQ 248

Query: 298 TIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSY 357
           TIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSY
Sbjct: 249 TIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSY 308

Query: 358 NFTYETKSKPAS 369
           NFTYETKSKPAS
Sbjct: 309 NFTYETKSKPAS 320

BLAST of MC07g_new0052 vs. NCBI nr
Match: XP_022150379.1 (uncharacterized protein LOC111018555 [Momordica charantia])

HSP 1 Score: 524 bits (1350), Expect = 1.44e-182
Identity = 256/363 (70.52%), Postives = 304/363 (83.75%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           ++H AFRFRH++  + + L R G   G CL A    P D +L DL+TIID+  L+SIPKY
Sbjct: 104 NTHHAFRFRHIYLGFNIVLWRVGPPYGDCLRAQWSDP-DRDLCDLSTIIDWGTLLSIPKY 163

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           +AFKG NGC+LSSRWIEGHQYHQF+S+DIGD  + METFITKDG+IR+KSNYFGKFWRRS
Sbjct: 164 IAFKGDNGCFLSSRWIEGHQYHQFASNDIGDPTIGMETFITKDGSIRVKSNYFGKFWRRS 223

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNH-VIALRNLGNKNFIKRFTYEYKTSCLNAATDS 185
           PNWIWADSTDT+T++PDTLF PTK+DN+ VIALRNLGN NFIKR T E KTSCLNAA ++
Sbjct: 224 PNWIWADSTDTSTNNPDTLFWPTKVDNNNVIALRNLGNNNFIKRLTTEGKTSCLNAAINT 283

Query: 186 IDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI 245
           ID  +RL M EP+L REIY++N+  ADSR+YDQ  LVMATG+ TNRT+V NTMNLNLSY 
Sbjct: 284 IDETARLQMVEPVLRREIYNVNYRLADSRVYDQIPLVMATGDATNRTQVANTMNLNLSYT 343

Query: 246 ETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQ 305
           ET+SSTWSSS+S KLGVKT++ETG+P I DGKVE+S+EF+GSYQWG+T T +QT+G+TYQ
Sbjct: 344 ETKSSTWSSSVSTKLGVKTTLETGVPFIADGKVEISAEFSGSYQWGETKTTSQTLGTTYQ 403

Query: 306 ITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETK 365
           +TVPP +TVIVSLLAT G CDVPYSY+Q DTLINGE K YEMDDGVY G+N YNF YE K
Sbjct: 404 VTVPPMSTVIVSLLATKGTCDVPYSYTQCDTLINGETKIYEMDDGVYTGINCYNFKYEVK 463

Query: 366 SKP 367
           SKP
Sbjct: 464 SKP 465

BLAST of MC07g_new0052 vs. NCBI nr
Match: XP_022953936.1 (uncharacterized protein LOC111456344 [Cucurbita moschata])

HSP 1 Score: 494 bits (1272), Expect = 9.74e-171
Identity = 243/362 (67.13%), Postives = 291/362 (80.39%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           S+HQAFRFRHV+  + V L R       CL A    P D +L DL+T ID+  L S+PK+
Sbjct: 104 SNHQAFRFRHVYLGFNVCLWRGSAPYVNCLRAQWSSP-DQDLCDLSTPIDWGTLQSLPKH 163

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           + FKG N CYLS+RWIEGHQY QF+S+DIGD  V METFITKDG+IR+KSNYFGKFWRRS
Sbjct: 164 IVFKGDNDCYLSARWIEGHQYLQFASNDIGDPTVGMETFITKDGHIRLKSNYFGKFWRRS 223

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSCLNAATDSI 185
           PNWIWADSTDTTT++PDTLF PTK+D +V+ALRN+GN NFIKR T E KTSCLNAA  +I
Sbjct: 224 PNWIWADSTDTTTNNPDTLFLPTKVDKNVVALRNMGNNNFIKRLTTEGKTSCLNAAVRTI 283

Query: 186 DAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYIE 245
           D  +RL  EE +L REIY++ F P+DSRIYDQ+ LVMAT N TN T+ P+TMNL L+Y E
Sbjct: 284 DVTARLQFEEAVLSREIYNVKFRPSDSRIYDQSTLVMATENATNTTKEPHTMNLKLTYTE 343

Query: 246 TESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQI 305
           T+SS W+SS+S+KLGVKT+IETGIP I +GKVE+S+EF+G YQWG+T T ++ + ++YQ+
Sbjct: 344 TKSSMWNSSVSMKLGVKTTIETGIPFIFEGKVEISAEFSGQYQWGETKTTSKAVETSYQV 403

Query: 306 TVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETKS 365
           TVPP TTVIVSLLAT G  DVPYSY+QRDTLINGE K Y+MDDGVY GVNSYNF YETKS
Sbjct: 404 TVPPMTTVIVSLLATKGTSDVPYSYTQRDTLINGETKTYQMDDGVYKGVNSYNFKYETKS 463

Query: 366 KP 367
            P
Sbjct: 464 TP 464

BLAST of MC07g_new0052 vs. NCBI nr
Match: XP_023520858.1 (uncharacterized protein LOC111784384 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 473 bits (1216), Expect = 3.07e-162
Identity = 234/362 (64.64%), Postives = 287/362 (79.28%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           S++QAFRFRHV+  + V L R       CL A    P D +L DL+T I++  L+S+PK+
Sbjct: 104 SNNQAFRFRHVYLGFNVCLWRGSAPYVNCLRAQWSTP-DGDLCDLSTPINWGTLLSLPKH 163

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           + FKG NG YLS++WIE HQY QF+S+DIGD  V METFITKDG+IR+KSNYFGKFWRRS
Sbjct: 164 IVFKGDNGYYLSAQWIEEHQYLQFASNDIGDPTVGMETFITKDGHIRLKSNYFGKFWRRS 223

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSCLNAATDSI 185
           PNWIWADSTDTTT++ DTLF PTK+D +V+ALRN+GN NFIKR T + KTSCLNAA ++I
Sbjct: 224 PNWIWADSTDTTTNNSDTLFLPTKVDKNVVALRNMGNNNFIKRLTADGKTSCLNAAVNTI 283

Query: 186 DAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYIE 245
           D  +RL  EE +L REIY++ F P+DSRIYDQ+ LVMAT   TN T+  +TMNL  +Y E
Sbjct: 284 DGTARLQFEESVLSREIYNVKFRPSDSRIYDQSTLVMATETATNTTKESHTMNLKFTYTE 343

Query: 246 TESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQI 305
           T+SS W+SS+SIKLGVKT+++TGIP IVDGKVE+S+ F+G YQWG+T T ++ + + YQ+
Sbjct: 344 TKSSMWNSSVSIKLGVKTTVQTGIPFIVDGKVEISATFSGQYQWGETKTTSKVVETLYQV 403

Query: 306 TVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETKS 365
           TVP  TTVIVSLLAT G  DVPYSY+Q DTLINGEIK Y+MDDGVY GVNSYNF YETKS
Sbjct: 404 TVPSMTTVIVSLLATQGTSDVPYSYTQCDTLINGEIKSYQMDDGVYKGVNSYNFKYETKS 463

Query: 366 KP 367
           KP
Sbjct: 464 KP 464

BLAST of MC07g_new0052 vs. ExPASy TrEMBL
Match: A0A6J1DAK2 (uncharacterized protein LOC111018553 OS=Momordica charantia OX=3673 GN=LOC111018553 PE=3 SV=1)

HSP 1 Score: 667 bits (1722), Expect = 2.36e-239
Identity = 325/365 (89.04%), Postives = 347/365 (95.07%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           SSHQAFRFRHVF+ YFV L + GFA+G CL A+ P+P+D +LGDLNT+IDYEALVSIPKY
Sbjct: 111 SSHQAFRFRHVFTGYFVALWQVGFAEGNCLRASFPYPDDCSLGDLNTVIDYEALVSIPKY 170

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           VAFKG N CYLSSRWIEGH+YHQFSSSD+GDSRVRMETFITKDGNIRIKSNYFGKFWRRS
Sbjct: 171 VAFKGDNDCYLSSRWIEGHEYHQFSSSDLGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 230

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNH-VIALRNLGNKNFIKRFTYEYKTSCLNAATDS 185
           PNWIWADSTDT+T++PDTLFCPTK+DN+ VIALRNLGN NFIKR T E KTSCLNAATDS
Sbjct: 231 PNWIWADSTDTSTNNPDTLFCPTKVDNNNVIALRNLGNNNFIKRLTTEGKTSCLNAATDS 290

Query: 186 IDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI 245
           IDAYSRL MEEP+LEREIY+INFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI
Sbjct: 291 IDAYSRLQMEEPVLEREIYNINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI 350

Query: 246 ETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQ 305
           ETESS WSSSISIKLGVKT+I+TGIPRIVDGKVEVSSE TGSYQWGKTITKTQT+GSTYQ
Sbjct: 351 ETESSAWSSSISIKLGVKTTIQTGIPRIVDGKVEVSSELTGSYQWGKTITKTQTVGSTYQ 410

Query: 306 ITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETK 365
           +TVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVN+YNFTYETK
Sbjct: 411 VTVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNNYNFTYETK 470

Query: 366 SKPAS 369
           SKPAS
Sbjct: 471 SKPAS 475

BLAST of MC07g_new0052 vs. ExPASy TrEMBL
Match: A0A6J1D9A4 (natterin-2-like OS=Momordica charantia OX=3673 GN=LOC111018554 PE=3 SV=1)

HSP 1 Score: 634 bits (1636), Expect = 9.61e-229
Identity = 311/312 (99.68%), Postives = 312/312 (100.00%), Query Frame = 0

Query: 58  ALVSIPKYVAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNY 117
           +LVSIPKYVAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNY
Sbjct: 9   SLVSIPKYVAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNY 68

Query: 118 FGKFWRRSPNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSC 177
           FGKFWRRSPNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSC
Sbjct: 69  FGKFWRRSPNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSC 128

Query: 178 LNAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTM 237
           LNAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTM
Sbjct: 129 LNAATDSIDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTM 188

Query: 238 NLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQ 297
           NLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQ
Sbjct: 189 NLNLSYIETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQ 248

Query: 298 TIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSY 357
           TIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSY
Sbjct: 249 TIGSTYQITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSY 308

Query: 358 NFTYETKSKPAS 369
           NFTYETKSKPAS
Sbjct: 309 NFTYETKSKPAS 320

BLAST of MC07g_new0052 vs. ExPASy TrEMBL
Match: A0A6J1D9W8 (uncharacterized protein LOC111018555 OS=Momordica charantia OX=3673 GN=LOC111018555 PE=3 SV=1)

HSP 1 Score: 524 bits (1350), Expect = 6.96e-183
Identity = 256/363 (70.52%), Postives = 304/363 (83.75%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           ++H AFRFRH++  + + L R G   G CL A    P D +L DL+TIID+  L+SIPKY
Sbjct: 104 NTHHAFRFRHIYLGFNIVLWRVGPPYGDCLRAQWSDP-DRDLCDLSTIIDWGTLLSIPKY 163

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           +AFKG NGC+LSSRWIEGHQYHQF+S+DIGD  + METFITKDG+IR+KSNYFGKFWRRS
Sbjct: 164 IAFKGDNGCFLSSRWIEGHQYHQFASNDIGDPTIGMETFITKDGSIRVKSNYFGKFWRRS 223

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNH-VIALRNLGNKNFIKRFTYEYKTSCLNAATDS 185
           PNWIWADSTDT+T++PDTLF PTK+DN+ VIALRNLGN NFIKR T E KTSCLNAA ++
Sbjct: 224 PNWIWADSTDTSTNNPDTLFWPTKVDNNNVIALRNLGNNNFIKRLTTEGKTSCLNAAINT 283

Query: 186 IDAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYI 245
           ID  +RL M EP+L REIY++N+  ADSR+YDQ  LVMATG+ TNRT+V NTMNLNLSY 
Sbjct: 284 IDETARLQMVEPVLRREIYNVNYRLADSRVYDQIPLVMATGDATNRTQVANTMNLNLSYT 343

Query: 246 ETESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQ 305
           ET+SSTWSSS+S KLGVKT++ETG+P I DGKVE+S+EF+GSYQWG+T T +QT+G+TYQ
Sbjct: 344 ETKSSTWSSSVSTKLGVKTTLETGVPFIADGKVEISAEFSGSYQWGETKTTSQTLGTTYQ 403

Query: 306 ITVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETK 365
           +TVPP +TVIVSLLAT G CDVPYSY+Q DTLINGE K YEMDDGVY G+N YNF YE K
Sbjct: 404 VTVPPMSTVIVSLLATKGTCDVPYSYTQCDTLINGETKIYEMDDGVYTGINCYNFKYEVK 463

Query: 366 SKP 367
           SKP
Sbjct: 464 SKP 465

BLAST of MC07g_new0052 vs. ExPASy TrEMBL
Match: A0A6J1GPH4 (uncharacterized protein LOC111456344 OS=Cucurbita moschata OX=3662 GN=LOC111456344 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 4.71e-171
Identity = 243/362 (67.13%), Postives = 291/362 (80.39%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           S+HQAFRFRHV+  + V L R       CL A    P D +L DL+T ID+  L S+PK+
Sbjct: 104 SNHQAFRFRHVYLGFNVCLWRGSAPYVNCLRAQWSSP-DQDLCDLSTPIDWGTLQSLPKH 163

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           + FKG N CYLS+RWIEGHQY QF+S+DIGD  V METFITKDG+IR+KSNYFGKFWRRS
Sbjct: 164 IVFKGDNDCYLSARWIEGHQYLQFASNDIGDPTVGMETFITKDGHIRLKSNYFGKFWRRS 223

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSCLNAATDSI 185
           PNWIWADSTDTTT++PDTLF PTK+D +V+ALRN+GN NFIKR T E KTSCLNAA  +I
Sbjct: 224 PNWIWADSTDTTTNNPDTLFLPTKVDKNVVALRNMGNNNFIKRLTTEGKTSCLNAAVRTI 283

Query: 186 DAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYIE 245
           D  +RL  EE +L REIY++ F P+DSRIYDQ+ LVMAT N TN T+ P+TMNL L+Y E
Sbjct: 284 DVTARLQFEEAVLSREIYNVKFRPSDSRIYDQSTLVMATENATNTTKEPHTMNLKLTYTE 343

Query: 246 TESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQI 305
           T+SS W+SS+S+KLGVKT+IETGIP I +GKVE+S+EF+G YQWG+T T ++ + ++YQ+
Sbjct: 344 TKSSMWNSSVSMKLGVKTTIETGIPFIFEGKVEISAEFSGQYQWGETKTTSKAVETSYQV 403

Query: 306 TVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETKS 365
           TVPP TTVIVSLLAT G  DVPYSY+QRDTLINGE K Y+MDDGVY GVNSYNF YETKS
Sbjct: 404 TVPPMTTVIVSLLATKGTSDVPYSYTQRDTLINGETKTYQMDDGVYKGVNSYNFKYETKS 463

Query: 366 KP 367
            P
Sbjct: 464 TP 464

BLAST of MC07g_new0052 vs. ExPASy TrEMBL
Match: A0A6J1GRC7 (uncharacterized protein LOC111456342 OS=Cucurbita moschata OX=3662 GN=LOC111456342 PE=4 SV=1)

HSP 1 Score: 471 bits (1213), Expect = 4.24e-162
Identity = 234/362 (64.64%), Postives = 284/362 (78.45%), Query Frame = 0

Query: 6   SSHQAFRFRHVFSNYFVTLCRDGFAQGKCLYANLPFPNDWNLGDLNTIIDYEALVSIPKY 65
           S+HQAFRFRHV+  + V L R       CL A    P D +L DL+T I++  L+S+PK+
Sbjct: 104 SNHQAFRFRHVYLGFNVCLWRGSAPYVNCLRAQWSSP-DRDLCDLSTPINWGTLLSLPKH 163

Query: 66  VAFKGHNGCYLSSRWIEGHQYHQFSSSDIGDSRVRMETFITKDGNIRIKSNYFGKFWRRS 125
           + FKG NG YLS++WIE HQY QF+S+DIGD  V METFITKDG+IR KSNYFGKFWRRS
Sbjct: 164 IVFKGDNGYYLSAQWIEEHQYLQFASNDIGDPTVGMETFITKDGHIRFKSNYFGKFWRRS 223

Query: 126 PNWIWADSTDTTTDDPDTLFCPTKIDNHVIALRNLGNKNFIKRFTYEYKTSCLNAATDSI 185
           PNWIWADSTDTTT++ DTLF PTK+D +V+ALRN+GN NFIKR T + KTSCLNAA  +I
Sbjct: 224 PNWIWADSTDTTTNNSDTLFLPTKVDKNVVALRNMGNNNFIKRLTADGKTSCLNAAVSTI 283

Query: 186 DAYSRLHMEEPILEREIYDINFHPADSRIYDQTVLVMATGNGTNRTEVPNTMNLNLSYIE 245
           D  +RL  EE +L REIY++ F P+DSRIYDQ+ LVMAT   TN T+  +TMNL  +Y E
Sbjct: 284 DGTARLQFEESVLSREIYNVKFRPSDSRIYDQSTLVMATETATNTTKESHTMNLKFTYTE 343

Query: 246 TESSTWSSSISIKLGVKTSIETGIPRIVDGKVEVSSEFTGSYQWGKTITKTQTIGSTYQI 305
           T+SS W+SS+SIKLGVKT+++TGIP IVDGKV++S+ F+G YQWG T T ++ + + YQ+
Sbjct: 344 TKSSMWNSSVSIKLGVKTTVQTGIPFIVDGKVKISATFSGQYQWGATKTTSKVVETLYQV 403

Query: 306 TVPPKTTVIVSLLATTGKCDVPYSYSQRDTLINGEIKYYEMDDGVYHGVNSYNFTYETKS 365
           TVP  TTVIVSLLAT G  DVPYSY+Q DTLINGEIK Y+MDDGVY GVNSYNF YETKS
Sbjct: 404 TVPSMTTVIVSLLATQGTSDVPYSYAQCDTLINGEIKSYQMDDGVYKGVNSYNFKYETKS 463

Query: 366 KP 367
           KP
Sbjct: 464 KP 464

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q66S215.6e-0926.47Natterin-2 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1[more]
Q66S253.6e-0828.00Natterin-1 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022150377.14.88e-23989.04uncharacterized protein LOC111018553 [Momordica charantia][more]
XP_022150378.11.99e-22899.68natterin-2-like [Momordica charantia][more]
XP_022150379.11.44e-18270.52uncharacterized protein LOC111018555 [Momordica charantia][more]
XP_022953936.19.74e-17167.13uncharacterized protein LOC111456344 [Cucurbita moschata][more]
XP_023520858.13.07e-16264.64uncharacterized protein LOC111784384 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DAK22.36e-23989.04uncharacterized protein LOC111018553 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1D9A49.61e-22999.68natterin-2-like OS=Momordica charantia OX=3673 GN=LOC111018554 PE=3 SV=1[more]
A0A6J1D9W86.96e-18370.52uncharacterized protein LOC111018555 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A6J1GPH44.71e-17167.13uncharacterized protein LOC111456344 OS=Cucurbita moschata OX=3662 GN=LOC1114563... [more]
A0A6J1GRC74.24e-16264.64uncharacterized protein LOC111456342 OS=Cucurbita moschata OX=3662 GN=LOC1114563... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008998Agglutinin domainSMARTSM00791agglutinincoord: 60..195
e-value: 1.2E-47
score: 174.3
IPR008998Agglutinin domainPFAMPF07468Agglutinincoord: 65..189
e-value: 5.2E-18
score: 65.7
IPR005830AerolysinPFAMPF01117Aerolysincoord: 190..330
e-value: 5.1E-7
score: 29.2
NoneNo IPR availableGENE3D2.80.10.50coord: 62..196
e-value: 5.3E-45
score: 154.8
NoneNo IPR availableGENE3D2.170.15.10Proaerolysin, chain A, domain 3coord: 200..366
e-value: 2.8E-49
score: 168.9
NoneNo IPR availablePANTHERPTHR39244NATTERIN-4coord: 8..367
NoneNo IPR availableCDDcd20216PFM_HFR-2-likecoord: 214..364
e-value: 1.35479E-71
score: 218.611
NoneNo IPR availableSUPERFAMILY56973Aerolisin/ETX pore-forming domaincoord: 101..364
IPR036242Agglutinin domain superfamilySUPERFAMILY50382Agglutinincoord: 59..195

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g_new0052.1MC07g_new0052.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region