MC07g0784 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0784
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	protein RALF-like 33
Location	MC07: 15460361 .. 15461385 (+)
RNA-Seq Expression	MC07g0784
Synteny	MC07g0784

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAATCTCACGCGTCTCTTCTCTCCTAGCGGCGTTCGCCATTTTCGCCGCTCATCTTCTGCTCTCATCGGCGACGGCCGCTGATTTCTCCGGCGACCACAAGCTCCTCTACGTGCCGGCCAAATCGGAGTGCCGAGGCTCAATCGCCGAGTGCCTGCTCGCCGGAGACGATTCCGGCTTCGGAACGGAGTTCGAGATGGACTCGGAGATCAATCGGCGAATTCTAGCGACTATGCGGTACATCAGCTACGGCGCGCTGAAGAGGAACAGCGTTCCTTGCTCCCGCCGCGGCGCGTCTTACTACAACTGCCAGCCCGGAGCTCAGGCGAATCCTTACAACCGCGGTTGCAGCGCAATTACTCGCTGCCGGAGCTAATTTGACCTGATTTTTTTTTATCTAGATTCATCGTCGTGTTCTATCGGTTAGTAATTCGTGTGTTTATACTGAATATACATGTCGATTGAGTAATAAGCTCCATACTGATGATTGAAAATGTAATCTACATCTGTTTCTGAGTCTTCTCTCCATGATTTATTTTCCTTTTTCGTGGCTGATTCTGGTTATTTCCAGAAACATTCGTCCTTGTTTGCAATCGATAATACAGTGGTAGGAACACAGCATTTGTGTAGTGTATTTTACAGATGGATCTTGCTGAACTCGCAGGGCCATTATTTTAGTTCTTTATTTGTTCATTTTATATTAATTTTTCTATTCGTTTATTTATTTATTTATTTTTGGTTGGTTGAGTTTTTGGCTGTCAAGATTGGGCTATCCGAATGGATGCAGATTTTCTACGTTGATTATCATTATCTTTCACTCTTGTTTTCTGAAAAAAAAAATATTTGTTGGGTAACGAATTTAGTGTGTGTGTGTGTATATATATATATATATATATTTCAATATTGTCCATATGAGTCTGAGCTCAAGTTAAAAGTATTAGTAATTTCATTTTTTTTCTCTAATTTCTAATTTTTTAAAAATATAAGAGTTTAGTAATTTTGAAAACAACAAAATCAAGTTAG

mRNA sequence

ATGGAAATCTCACGCGTCTCTTCTCTCCTAGCGGCGTTCGCCATTTTCGCCGCTCATCTTCTGCTCTCATCGGCGACGGCCGCTGATTTCTCCGGCGACCACAAGCTCCTCTACGTGCCGGCCAAATCGGAGTGCCGAGGCTCAATCGCCGAGTGCCTGCTCGCCGGAGACGATTCCGGCTTCGGAACGGAGTTCGAGATGGACTCGGAGATCAATCGGCGAATTCTAGCGACTATGCGGTACATCAGCTACGGCGCGCTGAAGAGGAACAGCGTTCCTTGCTCCCGCCGCGGCGCGTCTTACTACAACTGCCAGCCCGGAGCTCAGGCGAATCCTTACAACCGCGGTTGCAGCGCAATTACTCGCTGCCGGAGCTAATTTGACCTGATTTTTTTTTATCTAGATTCATCGTCGTGTTCTATCGGTTAGTAATTCGTGTGTTTATACTGAATATACATGTCGATTGAGTAATAAGCTCCATACTGATGATTGAAAATGTAATCTACATCTGTTTCTGAGTCTTCTCTCCATGATTTATTTTCCTTTTTCGTGGCTGATTCTGGTTATTTCCAGAAACATTCGTCCTTGTTTGCAATCGATAATACAGTGGTAGGAACACAGCATTTGTGTAGTGTATTTTACAGATGGATCTTGCTGAACTCGCAGGGCCATTATTTTAGTTCTTTATTTGTTCATTTTATATTAATTTTTCTATTCGTTTATTTATTTATTTATTTTTGGTTGGTTGAGTTTTTGGCTGTCAAGATTGGGCTATCCGAATGGATGCAGATTTTCTACGTTGATTATCATTATCTTTCACTCTTGTTTTCTGAAAAAAAAAATATTTGTTGGGTAACGAATTTAGTGTGTGTGTGTGTATATATATATATATATATATTTCAATATTGTCCATATGAGTCTGAGCTCAAGTTAAAAGTATTAGTAATTTCATTTTTTTTCTCTAATTTCTAATTTTTTAAAAATATAAGAGTTTAGTAATTTTGAAAACAACAAAATCAAGTTAG

Coding sequence (CDS)

ATGGAAATCTCACGCGTCTCTTCTCTCCTAGCGGCGTTCGCCATTTTCGCCGCTCATCTTCTGCTCTCATCGGCGACGGCCGCTGATTTCTCCGGCGACCACAAGCTCCTCTACGTGCCGGCCAAATCGGAGTGCCGAGGCTCAATCGCCGAGTGCCTGCTCGCCGGAGACGATTCCGGCTTCGGAACGGAGTTCGAGATGGACTCGGAGATCAATCGGCGAATTCTAGCGACTATGCGGTACATCAGCTACGGCGCGCTGAAGAGGAACAGCGTTCCTTGCTCCCGCCGCGGCGCGTCTTACTACAACTGCCAGCCCGGAGCTCAGGCGAATCCTTACAACCGCGGTTGCAGCGCAATTACTCGCTGCCGGAGCTAA

Protein sequence

MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAITRCRS

Homology

[Close]

BLAST of MC07g0784 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 3.5e-32
Identity = 72/111 (64.86%), Postives = 86/111 (77.48%), Query Frame = 0


Query: 14  AIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSGFGTEFEMDSEINR 73
           AI   H L ++ T+   SGD    +VP +S+C G+IAEC L+  +     EFEMDSEINR
Sbjct: 14  AILTVHFLFAAVTSQS-SGD----FVPIESKCNGTIAECSLSTAEE----EFEMDSEINR 73

Query: 74  RILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAITRCR 125
           RILAT +YISYGAL+RN+VPCSRRGASYYNC+ GAQANPY+RGCSAITRCR
Sbjct: 74  RILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of MC07g0784 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 7.9e-32
Identity = 79/137 (57.66%), Postives = 96/137 (70.07%), Query Frame = 0


Query: 3   ISRVSSLLAAFA---IFAAH--LLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG- 62
           +SR S   A FA   I A H   +  S+ + +F+GD    + P ++ECRG+IAEC ++  
Sbjct: 4   LSRNSGAAAIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSAA 63

Query: 63  -DDSG--------FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPG 122
             D G         G EFEMDSEINRRILAT RYISYGAL+RN++PCSRRGASYYNC+ G
Sbjct: 64  LGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRG 123

Query: 123 AQANPYNRGCSAITRCR 125
           AQANPY+RGCSAITRCR
Sbjct: 124 AQANPYSRGCSAITRCR 136

[Close]

BLAST of MC07g0784 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 1.6e-29
Identity = 68/104 (65.38%), Postives = 81/104 (77.88%), Query Frame = 0


Query: 24  SATAADFSGDHKLLYVPAKS--ECRGSIAECLLAGDDSGFGTEFEMDSEINRRILATMRY 83
           S  AA  SG +  + +PA+S   C+GSI EC+   +      EFE+DSE NRRILAT +Y
Sbjct: 19  SMAAAGDSGAYDWV-MPARSGGGCKGSIGECIAEEE------EFELDSESNRRILATKKY 78

Query: 84  ISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAITRCRS 126
           ISYGAL++NSVPCSRRGASYYNC+PGAQANPY+RGCSAITRCRS
Sbjct: 79  ISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of MC07g0784 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 3.4e-27
Identity = 61/83 (73.49%), Postives = 67/83 (80.72%), Query Frame = 0


Query: 43  SECRGSIAECLLAGDDSGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYY 102
           S C GSIAEC+ A ++       EMDSEINRRILAT +YISY +LKRNSVPCSRRGASYY
Sbjct: 45  SGCHGSIAECIGAEEE-------EMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYY 104

Query: 103 NCQPGAQANPYNRGCSAITRCRS 126
           NCQ GAQANPY+RGCS I RCRS
Sbjct: 105 NCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of MC07g0784 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 6.5e-26
Identity = 65/119 (54.62%), Postives = 79/119 (66.39%), Query Frame = 0


Query: 11  AAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSE-----CRGSIAECLLAGDDSGFGTEF 70
           A +A+ A   ++ SA  +       L +V A S      C GSIAEC+   +      E 
Sbjct: 6   AIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE------EM 65

Query: 71  EMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAITRCR 125
           E DS+I+RRILA  +YISYGA++RNSVPCSRRGASYYNCQ GAQANPY+RGCS ITRCR
Sbjct: 66  EFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCR 118

[Close]

BLAST of MC07g0784 vs. NCBI nr
Match: XP_022138421.1 (protein RALF-like 33 [Momordica charantia])

HSP 1 Score: 248 bits (633), Expect = 7.74e-83
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSG 60
           MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSG
Sbjct: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSG 60

Query: 61  FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAI 120
           FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAI
Sbjct: 61  FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAI 120

Query: 121 TRCRS 125
           TRCRS
Sbjct: 121 TRCRS 125

[Close]

BLAST of MC07g0784 vs. NCBI nr
Match: XP_038875155.1 (protein RALF-like 33 [Benincasa hispida])

HSP 1 Score: 210 bits (534), Expect = 1.04e-67
Identity = 107/127 (84.25%), Postives = 113/127 (88.98%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG--DD 60
           M ISRVSSLLAA AI AAH L+SSATA DFSGDHKLL+VP KSECRGSIAEC LAG  DD
Sbjct: 1   MGISRVSSLLAAVAILAAHFLISSATAVDFSGDHKLLFVPTKSECRGSIAECFLAGNDDD 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           S FG EF MDSEINRRILAT RYISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGC+
Sbjct: 61  SAFGMEFVMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCN 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. NCBI nr
Match: XP_023006467.1 (protein RALF-like 33 [Cucurbita maxima])

HSP 1 Score: 208 bits (529), Expect = 6.00e-67
Identity = 103/127 (81.10%), Postives = 114/127 (89.76%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG--DD 60
           M ISRVS+L+AAFA+ AAH L+SSATA DFSGD+++L+ P KSECRGSI EC LAG  DD
Sbjct: 1   MGISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDD 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           SGFG EFEMDSEINRRILAT RYISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCS
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. NCBI nr
Match: XP_023547686.1 (protein RALF-like 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 208 bits (529), Expect = 6.00e-67
Identity = 103/127 (81.10%), Postives = 114/127 (89.76%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG--DD 60
           M ISRVS+L+AAF + AAH L+SSATA DFSGD+++L+ P KSECRGSIAEC LAG  DD
Sbjct: 1   MGISRVSALIAAFVVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSIAECFLAGNNDD 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           SGFG EFEMDSEINRRILAT RYISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCS
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. NCBI nr
Match: KAG6575064.1 (Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 207 bits (527), Expect = 1.21e-66
Identity = 103/127 (81.10%), Postives = 114/127 (89.76%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG--DD 60
           M ISRVS+L+AAFA+ AAH L+ SATA DFSGD+++L+ P KSECRGSIAEC LAG  DD
Sbjct: 1   MGISRVSALIAAFAVLAAHFLILSATAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           SGFG EFEMDSEINRRILAT RYISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCS
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. ExPASy TrEMBL
Match: A0A6J1C9N4 (protein RALF-like 33 OS=Momordica charantia OX=3673 GN=LOC111009595 PE=3 SV=1)

HSP 1 Score: 248 bits (633), Expect = 3.75e-83
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSG 60
           MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSG
Sbjct: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSG 60

Query: 61  FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAI 120
           FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAI
Sbjct: 61  FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAI 120

Query: 121 TRCRS 125
           TRCRS
Sbjct: 121 TRCRS 125

[Close]

BLAST of MC07g0784 vs. ExPASy TrEMBL
Match: A0A6J1KVY0 (protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111499183 PE=3 SV=1)

HSP 1 Score: 208 bits (529), Expect = 2.90e-67
Identity = 103/127 (81.10%), Postives = 114/127 (89.76%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG--DD 60
           M ISRVS+L+AAFA+ AAH L+SSATA DFSGD+++L+ P KSECRGSI EC LAG  DD
Sbjct: 1   MGISRVSALIAAFAVLAAHFLISSATAIDFSGDNEILFAPTKSECRGSITECFLAGNYDD 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           SGFG EFEMDSEINRRILAT RYISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCS
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. ExPASy TrEMBL
Match: A0A6J1H3W7 (protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111460267 PE=3 SV=1)

HSP 1 Score: 205 bits (522), Expect = 3.39e-66
Identity = 102/127 (80.31%), Postives = 113/127 (88.98%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG--DD 60
           M ISRVS+L+AAFA+ AAH L+ SA A DFSGD+++L+ P KSECRGSIAEC LAG  DD
Sbjct: 1   MGISRVSALIAAFAVLAAHFLILSAAAIDFSGDNEILFAPTKSECRGSIAECFLAGNDDD 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           SGFG EFEMDSEINRRILAT RYISYGAL+RN+VPCSRRGASYYNC+PGAQANPY RGCS
Sbjct: 61  SGFGMEFEMDSEINRRILATTRYISYGALRRNNVPCSRRGASYYNCRPGAQANPYTRGCS 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. ExPASy TrEMBL
Match: A0A6J1KG89 (protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111494831 PE=3 SV=1)

HSP 1 Score: 204 bits (519), Expect = 9.72e-66
Identity = 105/127 (82.68%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDD-- 60
           MEISRV S LAA AI AAH LLSSA   DFSGD KLL+VPA+S+CRGSIAEC LAGDD  
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMVVDFSGDDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           S FG EFEMDSEINRRILA  RY+SYGALKRNSVPCSRRGASYYNCQPGAQANPY RGC+
Sbjct: 61  SAFGMEFEMDSEINRRILAASRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. ExPASy TrEMBL
Match: A0A6J1EV64 (protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111436882 PE=3 SV=1)

HSP 1 Score: 203 bits (517), Expect = 1.96e-65
Identity = 105/127 (82.68%), Postives = 111/127 (87.40%), Query Frame = 0


Query: 1   MEISRVSSLLAAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDD-- 60
           MEISRV S LAA AI AAH LLSSA A DFSG  KLL+VPA+S+CRGSIAEC LAGDD  
Sbjct: 1   MEISRVFSFLAASAIIAAHFLLSSAMAVDFSGGDKLLFVPAESKCRGSIAECFLAGDDND 60

Query: 61  SGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCS 120
           S FG EFEMDSEINRRILA+ RY+SYGALKRNSVPCSRRGASYYNCQPGAQANPY RGC+
Sbjct: 61  SAFGMEFEMDSEINRRILASSRYLSYGALKRNSVPCSRRGASYYNCQPGAQANPYRRGCN 120

Query: 121 AITRCRS 125
           AITRCRS
Sbjct: 121 AITRCRS 127

[Close]

BLAST of MC07g0784 vs. TAIR 10
Match: AT4G15800.1 (ralf-like 33 )

HSP 1 Score: 139.0 bits (349), Expect = 2.5e-33
Identity = 72/111 (64.86%), Postives = 86/111 (77.48%), Query Frame = 0


Query: 14  AIFAAHLLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAGDDSGFGTEFEMDSEINR 73
           AI   H L ++ T+   SGD    +VP +S+C G+IAEC L+  +     EFEMDSEINR
Sbjct: 14  AILTVHFLFAAVTSQS-SGD----FVPIESKCNGTIAECSLSTAEE----EFEMDSEINR 73

Query: 74  RILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAITRCR 125
           RILAT +YISYGAL+RN+VPCSRRGASYYNC+ GAQANPY+RGCSAITRCR
Sbjct: 74  RILATTKYISYGALRRNTVPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of MC07g0784 vs. TAIR 10
Match: AT3G16570.1 (rapid alkalinization factor 23 )

HSP 1 Score: 137.9 bits (346), Expect = 5.6e-33
Identity = 79/137 (57.66%), Postives = 96/137 (70.07%), Query Frame = 0


Query: 3   ISRVSSLLAAFA---IFAAH--LLLSSATAADFSGDHKLLYVPAKSECRGSIAECLLAG- 62
           +SR S   A FA   I A H   +  S+ + +F+GD    + P ++ECRG+IAEC ++  
Sbjct: 4   LSRNSGAAAIFAILLILAVHNWSVAVSSQSTEFAGD----FPPFETECRGTIAECSVSAA 63

Query: 63  -DDSG--------FGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPG 122
             D G         G EFEMDSEINRRILAT RYISYGAL+RN++PCSRRGASYYNC+ G
Sbjct: 64  LGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRRNTIPCSRRGASYYNCRRG 123

Query: 123 AQANPYNRGCSAITRCR 125
           AQANPY+RGCSAITRCR
Sbjct: 124 AQANPYSRGCSAITRCR 136

[Close]

BLAST of MC07g0784 vs. TAIR 10
Match: AT1G02900.1 (rapid alkalinization factor 1 )

HSP 1 Score: 122.5 bits (306), Expect = 2.4e-28
Identity = 61/83 (73.49%), Postives = 67/83 (80.72%), Query Frame = 0


Query: 43  SECRGSIAECLLAGDDSGFGTEFEMDSEINRRILATMRYISYGALKRNSVPCSRRGASYY 102
           S C GSIAEC+ A ++       EMDSEINRRILAT +YISY +LKRNSVPCSRRGASYY
Sbjct: 45  SGCHGSIAECIGAEEE-------EMDSEINRRILATTKYISYQSLKRNSVPCSRRGASYY 104

Query: 103 NCQPGAQANPYNRGCSAITRCRS 126
           NCQ GAQANPY+RGCS I RCRS
Sbjct: 105 NCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of MC07g0784 vs. TAIR 10
Match: AT3G05490.1 (ralf-like 22 )

HSP 1 Score: 118.2 bits (295), Expect = 4.6e-27
Identity = 65/119 (54.62%), Postives = 79/119 (66.39%), Query Frame = 0


Query: 11  AAFAIFAAHLLLSSATAADFSGDHKLLYVPAKSE-----CRGSIAECLLAGDDSGFGTEF 70
           A +A+ A   ++ SA  +       L +V A S      C GSIAEC+   +      E 
Sbjct: 6   AIYAVIAILAIVISAVESTGDFGDSLDFVRAGSSSLFSGCTGSIAECIAEEE------EM 65

Query: 71  EMDSEINRRILATMRYISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAITRCR 125
           E DS+I+RRILA  +YISYGA++RNSVPCSRRGASYYNCQ GAQANPY+RGCS ITRCR
Sbjct: 66  EFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGAQANPYSRGCSTITRCR 118

[Close]

BLAST of MC07g0784 vs. TAIR 10
Match: AT2G33775.1 (ralf-like 19 )

HSP 1 Score: 85.5 bits (210), Expect = 3.3e-17
Identity = 40/61 (65.57%), Postives = 48/61 (78.69%), Query Frame = 0


Query: 64  EFEMDSEINRRILATMR-YISYGALKRNSVPCSRRGASYYNCQPGAQANPYNRGCSAITR 123
           ++ MDSE NRR LA  R YISYGAL++N+VPCSRRG SYY+C+   +ANPY RGCS IT 
Sbjct: 45  DYLMDSETNRRQLAARRSYISYGALRKNNVPCSRRGRSYYDCKKRKRANPYRRGCSVITH 104

The following BLAST results are available for this feature:

BLAST of MC07g0784 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9P8	3.5e-32	64.86	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]
Q9LUS7	7.9e-32	57.66	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]
Q945T0	1.6e-29	65.38	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
Q9SRY3	3.4e-27	73.49	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
Q9MA62	6.5e-26	54.62	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]

BLAST of MC07g0784 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138421.1	7.74e-83	100.00	protein RALF-like 33 [Momordica charantia]	[more]
XP_038875155.1	1.04e-67	84.25	protein RALF-like 33 [Benincasa hispida]	[more]
XP_023006467.1	6.00e-67	81.10	protein RALF-like 33 [Cucurbita maxima]	[more]
XP_023547686.1	6.00e-67	81.10	protein RALF-like 33 [Cucurbita pepo subsp. pepo]	[more]
KAG6575064.1	1.21e-66	81.10	Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MC07g0784 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C9N4	3.75e-83	100.00	protein RALF-like 33 OS=Momordica charantia OX=3673 GN=LOC111009595 PE=3 SV=1	[more]
A0A6J1KVY0	2.90e-67	81.10	protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111499183 PE=3 SV=1	[more]
A0A6J1H3W7	3.39e-66	80.31	protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111460267 PE=3 SV=1	[more]
A0A6J1KG89	9.72e-66	82.68	protein RALF-like 33 OS=Cucurbita maxima OX=3661 GN=LOC111494831 PE=3 SV=1	[more]
A0A6J1EV64	1.96e-65	82.68	protein RALF-like 33 OS=Cucurbita moschata OX=3662 GN=LOC111436882 PE=3 SV=1	[more]

BLAST of MC07g0784 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15800.1	2.5e-33	64.86	ralf-like 33	[more]
AT3G16570.1	5.6e-33	57.66	rapid alkalinization factor 23	[more]
AT1G02900.1	2.4e-28	73.49	rapid alkalinization factor 1	[more]
AT3G05490.1	4.6e-27	54.62	ralf-like 22	[more]
AT2G33775.1	3.3e-17	65.57	ralf-like 19	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 64..124 e-value: 8.9E-31 score: 106.7
None	No IPR available	PANTHER	PTHR33136:SF50	PROTEIN RALF-LIKE 33	coord: 8..125
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 8..125

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0784.1	MC07g0784.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region