MC07g0764 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0764
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	E3 ubiquitin-protein ligase Topors
Location	MC07: 15339972 .. 15341371 (+)
RNA-Seq Expression	MC07g0764
Synteny	MC07g0764

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAGTGCAGGAGGAAATCATGAAGCCTGCAACCCGGGAACCGCGGCGAAAGCTCAACCGCGAAAACTTCGTATCTAAGGTAATTTCGCCGGCAATCTGTGGGGAAACCTGCCCAATTTGCCTCAGAGAATTGCAGGATTGTACCACCGCCGTGCTTACATCTTGTATCCACGCCTATTGCGTAGAATGCATTCGCAAATGGAGCACTTTGAAGCGGACTTGCCCTCTCTGCAACGCTCAATTCGAGTCATGGTTCTCCAAAATCAATTTCTCATCATCAAGCTTCCGCAAAGAACATTTGCCAACCTTGAATTGTGTCGATAAGCTCAATACTGGAGTCGGATCCTCCCGGATTGACAGTCGCAGGTGAGTTTTCTGATACGTCTTTTATGGCATCATGTTTTCGAATTGACATTTTCTGCTGGAATTTGAACATTGAAGCGAAGATGGAACTATATTTTCTTTGCTCTCTTCAGTTCCCGGAGAACTGCACGCAGTAGGCAGTTTCAAGCAAGAATTAGATTGAACATTGAACTTGAAGCTATATTTGTTCTCCTACTTTGATGTAGGCTTCTTCAGAGAGCGAGGAACGAATTAAATAGAGTTAGACGGTCGGAAATACCACTGACATGGCGGCGATCGTTTGGACGGCGAGGGTCAGAACCTGTACCAGCTGACGTTATTGCCCAGCGAAAACTTCAATGGCGAGCAAGGTATCTTCTACGTTTATTTCTCTGGTTGGATTTTACGGTTCTCACATTTTTTGCCCATGTTCTAGAGTTAGAGCTCGAATATTTGTTGCTAATTTTCTGTTGGGTTATGATTTGACAAAGCATATACAACCGACATATACAAGCTGTTCCATCTTCGGTCAGATGCGGTGCAGAACTGGTATGCATTTTAATCCTCTAGCAATTACGTTTATATAGAATTTCATTACCTCATATACAGCTTTGGGGGCCAATTCCTTGCTTCGAAATTGAATTGGACACTTGTTTTTTAATCAAATACATTGCAGATTGCCTACTTGCACAGTTTATAATAATTGATTTCATTTTCTCTTTGGGTGCACAATTTATAATTTCTCATTGGGTTCAATTCTGTTGATTTGGTTCTTCTAATGCTGTGTTTGTGACGTTTAAATAGAACATATCAGGAAGTCATGGAGCTAAAGAAGCAATACTAGAGAAGATTAAACCATGGATTCAGAGAGAGTTGCAAGCCATCCTTGAGGACCCAGATCCAACTATAATTGTTCATCTGGTTACCTCACTATTTGTCTCGAGACTTGAAGCTCCTTCCTCGCAGCTTAATCCCGAGGATGACTTTCTTTTGGCGCTGCATCCCTTCCTTTATGAAAAAACTGACTTGTTTTGGCATGAATTAAGGTACATGACTCAT

mRNA sequence

TTAGTGCAGGAGGAAATCATGAAGCCTGCAACCCGGGAACCGCGGCGAAAGCTCAACCGCGAAAACTTCGTATCTAAGGTAATTTCGCCGGCAATCTGTGGGGAAACCTGCCCAATTTGCCTCAGAGAATTGCAGGATTGTACCACCGCCGTGCTTACATCTTGTATCCACGCCTATTGCGTAGAATGCATTCGCAAATGGAGCACTTTGAAGCGGACTTGCCCTCTCTGCAACGCTCAATTCGAGTCATGGTTCTCCAAAATCAATTTCTCATCATCAAGCTTCCGCAAAGAACATTTGCCAACCTTGAATTGTGTCGATAAGCTCAATACTGGAGTCGGATCCTCCCGGATTGACAGTCGCAGGCTTCTTCAGAGAGCGAGGAACGAATTAAATAGAGTTAGACGGTCGGAAATACCACTGACATGGCGGCGATCGTTTGGACGGCGAGGGTCAGAACCTGTACCAGCTGACGTTATTGCCCAGCGAAAACTTCAATGGCGAGCAAGCATATACAACCGACATATACAAGCTGTTCCATCTTCGGTCAGATGCGGTGCAGAACTGAACATATCAGGAAGTCATGGAGCTAAAGAAGCAATACTAGAGAAGATTAAACCATGGATTCAGAGAGAGTTGCAAGCCATCCTTGAGGACCCAGATCCAACTATAATTGTTCATCTGGTTACCTCACTATTTGTCTCGAGACTTGAAGCTCCTTCCTCGCAGCTTAATCCCGAGGATGACTTTCTTTTGGCGCTGCATCCCTTCCTTTATGAAAAAACTGACTTGTTTTGGCATGAATTAAGGTACATGACTCAT

Coding sequence (CDS)

TTAGTGCAGGAGGAAATCATGAAGCCTGCAACCCGGGAACCGCGGCGAAAGCTCAACCGCGAAAACTTCGTATCTAAGGTAATTTCGCCGGCAATCTGTGGGGAAACCTGCCCAATTTGCCTCAGAGAATTGCAGGATTGTACCACCGCCGTGCTTACATCTTGTATCCACGCCTATTGCGTAGAATGCATTCGCAAATGGAGCACTTTGAAGCGGACTTGCCCTCTCTGCAACGCTCAATTCGAGTCATGGTTCTCCAAAATCAATTTCTCATCATCAAGCTTCCGCAAAGAACATTTGCCAACCTTGAATTGTGTCGATAAGCTCAATACTGGAGTCGGATCCTCCCGGATTGACAGTCGCAGGCTTCTTCAGAGAGCGAGGAACGAATTAAATAGAGTTAGACGGTCGGAAATACCACTGACATGGCGGCGATCGTTTGGACGGCGAGGGTCAGAACCTGTACCAGCTGACGTTATTGCCCAGCGAAAACTTCAATGGCGAGCAAGCATATACAACCGACATATACAAGCTGTTCCATCTTCGGTCAGATGCGGTGCAGAACTGAACATATCAGGAAGTCATGGAGCTAAAGAAGCAATACTAGAGAAGATTAAACCATGGATTCAGAGAGAGTTGCAAGCCATCCTTGAGGACCCAGATCCAACTATAATTGTTCATCTGGTTACCTCACTATTTGTCTCGAGACTTGAAGCTCCTTCCTCGCAGCTTAATCCCGAGGATGACTTTCTTTTGGCGCTGCATCCCTTCCTTTATGAAAAAACTGACTTGTTTTGGCATGAATTAAGGTACATGACTCAT

Protein sequence

LVQEEIMKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQRARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCGAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNPEDDFLLALHPFLYEKTDLFWHELRYMTH

Homology

[Close]

BLAST of MC07g0764 vs. ExPASy Swiss-Prot
Match: P08393 (E3 ubiquitin-protein ligase ICP0 OS=Human herpesvirus 1 (strain 17) OX=10299 GN=ICP0 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 8.1e-05
Identity = 17/47 (36.17%), Postives = 27/47 (57.45%), Query Frame = 0


Query: 34  GETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKRTCPLCNAQ 81
           G+ C +C  E+          C+H +C+ C++ W  L+ TCPLCNA+
Sbjct: 113 GDVCAVCTDEIAPHLRCDTFPCMHRFCIPCMKTWMQLRNTCPLCNAK 159

[Close]

BLAST of MC07g0764 vs. ExPASy Swiss-Prot
Match: P29129 (E3 ubiquitin-protein ligase ICP0 OS=Suid herpesvirus 1 (strain Indiana-Funkhauser / Becker) OX=31523 GN=EP0 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.4e-04
Identity = 21/57 (36.84%), Postives = 32/57 (56.14%), Query Frame = 0


Query: 37  CPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKRTCPLCNAQFESWFSKINFSSS 94
           CPICL      T A    C+H +C++CI++W+     CPLCNA+  S    ++  +S
Sbjct: 46  CPICLDVA--ATEAQTLPCMHKFCLDCIQRWTLTSTACPLCNARVTSILHHVDSDAS 100

[Close]

BLAST of MC07g0764 vs. ExPASy Swiss-Prot
Match: P28284 (E3 ubiquitin-protein ligase ICP0 OS=Human herpesvirus 2 (strain HG52) OX=10315 GN=RL2 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.1e-04
Identity = 16/45 (35.56%), Postives = 25/45 (55.56%), Query Frame = 0


Query: 34  GETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKRTCPLCN 79
           G+ C +C  E+          C+H +C+ C++ W  L+ TCPLCN
Sbjct: 123 GDVCAVCTDEIAPPLRCQSFPCLHPFCIPCMKTWIPLRNTCPLCN 167

[Close]

BLAST of MC07g0764 vs. ExPASy Swiss-Prot
Match: Q9P1Y6 (PHD and RING finger domain-containing protein 1 OS=Homo sapiens OX=9606 GN=PHRF1 PE=1 SV=3)

HSP 1 Score: 47.4 bits (111), Expect = 3.1e-04
Identity = 20/63 (31.75%), Postives = 31/63 (49.21%), Query Frame = 0


Query: 35  ETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKRTCPLCNAQFESWFSKINFSSSS 94
           E+CPICL   +D       +C H +C++CI +WS    +CP+    F+    +  F    
Sbjct: 106 ESCPICLNAFRDQAVGTPENCAHYFCLDCIVEWSKNANSCPVDRTLFKCICIRAQFGGKI 165

Query: 95  FRK 98
            RK
Sbjct: 166 LRK 168

[Close]

BLAST of MC07g0764 vs. ExPASy Swiss-Prot
Match: Q7ZWF4 (RING finger protein 145 OS=Danio rerio OX=7955 GN=rnf145 PE=2 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 4.0e-04
Identity = 20/49 (40.82%), Postives = 30/49 (61.22%), Query Frame = 0


Query: 35  ETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKRTCPLCNAQFES 84
           + C IC    QD  +AV+T C H +   C++KW  ++ TCPLC+ Q +S
Sbjct: 535 DICSIC---FQDMKSAVITPCSHFFHAACLKKWLYVQETCPLCHGQLKS 580

[Close]

BLAST of MC07g0764 vs. NCBI nr
Match: XP_022138780.1 (E3 ubiquitin-protein ligase Topors [Momordica charantia])

HSP 1 Score: 537 bits (1384), Expect = 3.17e-192
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0


Query: 7   MKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIRK 66
           MKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIRK
Sbjct: 1   MKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIRK 60

Query: 67  WSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQR 126
           WSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQR
Sbjct: 61  WSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQR 120

Query: 127 ARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCG 186
           ARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCG
Sbjct: 121 ARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCG 180

Query: 187 AELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNP 246
           AELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNP
Sbjct: 181 AELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNP 240

Query: 247 EDDFLLALHPFLYEKTDLFWHELR 270
           EDDFLLALHPFLYEKTDLFWHELR
Sbjct: 241 EDDFLLALHPFLYEKTDLFWHELR 264

[Close]

BLAST of MC07g0764 vs. NCBI nr
Match: XP_022999966.1 (E3 ubiquitin-protein ligase Topors isoform X1 [Cucurbita maxima])

HSP 1 Score: 426 bits (1094), Expect = 5.97e-148
Identity = 210/270 (77.78%), Postives = 231/270 (85.56%), Query Frame = 0


Query: 1   LVQEEIMKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYC 60
           +++ +IMK +T EP RKLN ENFV KVISPAICG+TCPICLREL+D T AVLT+CIHAYC
Sbjct: 1   MLEGKIMKHSTGEPARKLNGENFVVKVISPAICGQTCPICLRELEDRTAAVLTTCIHAYC 60

Query: 61  VECIRKWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDS 120
           V CIRKWS LKRTCPLCNAQF+SWF KIN SS SFRKE LP LNC D L  G+GSSR D+
Sbjct: 61  VGCIRKWSNLKRTCPLCNAQFDSWFCKINLSSRSFRKERLPNLNCADNLKIGIGSSRTDA 120

Query: 121 RRLLQRARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVP 180
           RR+LQ  RNEL+R RRSE PLTWRRSFGRRG E VP DVIA RKLQWRASIY+R IQAVP
Sbjct: 121 RRILQSTRNELSRDRRSERPLTWRRSFGRRGPESVPTDVIAWRKLQWRASIYDRRIQAVP 180

Query: 181 SSVRCGAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAP 240
           SSVR    +N++GSHG KEAILE+IKPWIQREL+AIL DPDPTIIVHLVTSLFV+RLEAP
Sbjct: 181 SSVRSCLVMNLTGSHGVKEAILERIKPWIQRELEAILGDPDPTIIVHLVTSLFVARLEAP 240

Query: 241 SSQLNPEDDFLLALHPFLYEKTDLFWHELR 270
           SSQLN E+DFL  L PFLYEKTDLFWHELR
Sbjct: 241 SSQLNAENDFLSPLRPFLYEKTDLFWHELR 270

[Close]

BLAST of MC07g0764 vs. NCBI nr
Match: XP_038875513.1 (E3 ubiquitin-protein ligase Topors isoform X2 [Benincasa hispida])

HSP 1 Score: 423 bits (1088), Expect = 3.19e-147
Identity = 211/270 (78.15%), Postives = 230/270 (85.19%), Query Frame = 0


Query: 1   LVQEEIMKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYC 60
           ++Q E+MKP+TREP  KLN ENF+SKVISPAICGETCPICL EL+D T AVLT+CIHAYC
Sbjct: 1   MLQAEVMKPSTREPAPKLNGENFISKVISPAICGETCPICLIELKDRTAAVLTTCIHAYC 60

Query: 61  VECIRKWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDS 120
           + CIRKWS LKRTCPLCNAQF+SWF+KIN SS SFR E L TLNC DKL  GVGSSR D+
Sbjct: 61  IGCIRKWSNLKRTCPLCNAQFDSWFAKINLSSRSFRIERLKTLNCPDKLKIGVGSSRTDA 120

Query: 121 RRLLQRARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVP 180
           R +LQ  RNELNR RRSE  LTWRRSFGRRG E VPADV+A R+LQWRASIYNR IQA+P
Sbjct: 121 RGILQSTRNELNRERRSERSLTWRRSFGRRGPESVPADVVAGRELQWRASIYNRCIQALP 180

Query: 181 SSVRCGAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAP 240
           SSVR   ELNI GS GAKEAILE+IKPWI+RELQAILEDPDPTIIVHLV SLFV+RLE P
Sbjct: 181 SSVRSCLELNILGSRGAKEAILERIKPWIRRELQAILEDPDPTIIVHLVISLFVARLEVP 240

Query: 241 SSQLNPEDDFLLALHPFLYEKTDLFWHELR 270
           S QLN EDDFL  L PFL+EKTDLFWHELR
Sbjct: 241 SPQLNAEDDFLSPLRPFLFEKTDLFWHELR 270

[Close]

BLAST of MC07g0764 vs. NCBI nr
Match: XP_038875512.1 (E3 ubiquitin-protein ligase Topors isoform X1 [Benincasa hispida])

HSP 1 Score: 423 bits (1088), Expect = 4.89e-147
Identity = 211/270 (78.15%), Postives = 230/270 (85.19%), Query Frame = 0


Query: 1   LVQEEIMKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYC 60
           ++Q E+MKP+TREP  KLN ENF+SKVISPAICGETCPICL EL+D T AVLT+CIHAYC
Sbjct: 1   MLQAEVMKPSTREPAPKLNGENFISKVISPAICGETCPICLIELKDRTAAVLTTCIHAYC 60

Query: 61  VECIRKWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDS 120
           + CIRKWS LKRTCPLCNAQF+SWF+KIN SS SFR E L TLNC DKL  GVGSSR D+
Sbjct: 61  IGCIRKWSNLKRTCPLCNAQFDSWFAKINLSSRSFRIERLKTLNCPDKLKIGVGSSRTDA 120

Query: 121 RRLLQRARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVP 180
           R +LQ  RNELNR RRSE  LTWRRSFGRRG E VPADV+A R+LQWRASIYNR IQA+P
Sbjct: 121 RGILQSTRNELNRERRSERSLTWRRSFGRRGPESVPADVVAGRELQWRASIYNRCIQALP 180

Query: 181 SSVRCGAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAP 240
           SSVR   ELNI GS GAKEAILE+IKPWI+RELQAILEDPDPTIIVHLV SLFV+RLE P
Sbjct: 181 SSVRSCLELNILGSRGAKEAILERIKPWIRRELQAILEDPDPTIIVHLVISLFVARLEVP 240

Query: 241 SSQLNPEDDFLLALHPFLYEKTDLFWHELR 270
           S QLN EDDFL  L PFL+EKTDLFWHELR
Sbjct: 241 SPQLNAEDDFLSPLRPFLFEKTDLFWHELR 270

[Close]

BLAST of MC07g0764 vs. NCBI nr
Match: KAG6593954.1 (E3 ubiquitin-protein ligase Topors, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 422 bits (1084), Expect = 1.99e-146
Identity = 209/265 (78.87%), Postives = 225/265 (84.91%), Query Frame = 0


Query: 6   IMKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIR 65
           IM  +TREP RKL  ENFV KVISPAICGETCPICLREL+D T AVLT+CIHAYCV CIR
Sbjct: 6   IMNHSTREPARKLYGENFVVKVISPAICGETCPICLRELEDRTAAVLTTCIHAYCVGCIR 65

Query: 66  KWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQ 125
           KWS LKRTCPLCNAQF+SWFSKI  SS SFRKE LP LNC D L  G+GSSR D+RR+LQ
Sbjct: 66  KWSNLKRTCPLCNAQFDSWFSKIKLSSRSFRKERLPNLNCADNLKIGIGSSRTDARRILQ 125

Query: 126 RARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRC 185
             RNEL+R RRSE PLTWRRSFGRRG E VP DVIA RKLQWRASIY+R IQA PSSVR 
Sbjct: 126 STRNELSRDRRSERPLTWRRSFGRRGPESVPTDVIAWRKLQWRASIYDRRIQAAPSSVRS 185

Query: 186 GAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLN 245
              +N++GSHG KEAILE+IKPWIQREL+AIL DPDPTIIVHLVTSLFV+RLEAPSSQLN
Sbjct: 186 CLVMNLTGSHGVKEAILERIKPWIQRELEAILGDPDPTIIVHLVTSLFVARLEAPSSQLN 245

Query: 246 PEDDFLLALHPFLYEKTDLFWHELR 270
            E+DFL  L PFLYEKTDLFWHELR
Sbjct: 246 AENDFLSPLRPFLYEKTDLFWHELR 270

[Close]

BLAST of MC07g0764 vs. ExPASy TrEMBL
Match: A0A6J1CAG7 (E3 ubiquitin-protein ligase Topors OS=Momordica charantia OX=3673 GN=LOC111009859 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 1.54e-192
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0


Query: 7   MKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIRK 66
           MKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIRK
Sbjct: 1   MKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIRK 60

Query: 67  WSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQR 126
           WSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQR
Sbjct: 61  WSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQR 120

Query: 127 ARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCG 186
           ARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCG
Sbjct: 121 ARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCG 180

Query: 187 AELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNP 246
           AELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNP
Sbjct: 181 AELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNP 240

Query: 247 EDDFLLALHPFLYEKTDLFWHELR 270
           EDDFLLALHPFLYEKTDLFWHELR
Sbjct: 241 EDDFLLALHPFLYEKTDLFWHELR 264

[Close]

BLAST of MC07g0764 vs. ExPASy TrEMBL
Match: A0A6J1KEK6 (E3 ubiquitin-protein ligase Topors isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111494293 PE=4 SV=1)

HSP 1 Score: 426 bits (1094), Expect = 2.89e-148
Identity = 210/270 (77.78%), Postives = 231/270 (85.56%), Query Frame = 0


Query: 1   LVQEEIMKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYC 60
           +++ +IMK +T EP RKLN ENFV KVISPAICG+TCPICLREL+D T AVLT+CIHAYC
Sbjct: 1   MLEGKIMKHSTGEPARKLNGENFVVKVISPAICGQTCPICLRELEDRTAAVLTTCIHAYC 60

Query: 61  VECIRKWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDS 120
           V CIRKWS LKRTCPLCNAQF+SWF KIN SS SFRKE LP LNC D L  G+GSSR D+
Sbjct: 61  VGCIRKWSNLKRTCPLCNAQFDSWFCKINLSSRSFRKERLPNLNCADNLKIGIGSSRTDA 120

Query: 121 RRLLQRARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVP 180
           RR+LQ  RNEL+R RRSE PLTWRRSFGRRG E VP DVIA RKLQWRASIY+R IQAVP
Sbjct: 121 RRILQSTRNELSRDRRSERPLTWRRSFGRRGPESVPTDVIAWRKLQWRASIYDRRIQAVP 180

Query: 181 SSVRCGAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAP 240
           SSVR    +N++GSHG KEAILE+IKPWIQREL+AIL DPDPTIIVHLVTSLFV+RLEAP
Sbjct: 181 SSVRSCLVMNLTGSHGVKEAILERIKPWIQRELEAILGDPDPTIIVHLVTSLFVARLEAP 240

Query: 241 SSQLNPEDDFLLALHPFLYEKTDLFWHELR 270
           SSQLN E+DFL  L PFLYEKTDLFWHELR
Sbjct: 241 SSQLNAENDFLSPLRPFLYEKTDLFWHELR 270

[Close]

BLAST of MC07g0764 vs. ExPASy TrEMBL
Match: A0A6J1EQ79 (E3 ubiquitin-protein ligase Topors isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436818 PE=4 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 1.94e-146
Identity = 209/265 (78.87%), Postives = 225/265 (84.91%), Query Frame = 0


Query: 6   IMKPATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECIR 65
           I K +T EP RKLN ENFV KVISPAICGETCPICLREL+D   AVLT+CIHAYCV CIR
Sbjct: 6   ITKHSTPEPARKLNGENFVVKVISPAICGETCPICLRELEDRIAAVLTTCIHAYCVGCIR 65

Query: 66  KWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQ 125
           KWS LKRTCPLCNAQF+SWFSKI  SS SFRKE LP LNC D L  G+GSSR D+RR+LQ
Sbjct: 66  KWSNLKRTCPLCNAQFDSWFSKIKLSSRSFRKERLPNLNCADNLKIGIGSSRTDARRILQ 125

Query: 126 RARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRC 185
             RNEL+R RRSE PLTWRRSFGRRG E VP DVIA RKLQWRASIY+R IQAVPSSVR 
Sbjct: 126 STRNELSRDRRSERPLTWRRSFGRRGPESVPTDVIAWRKLQWRASIYDRRIQAVPSSVRS 185

Query: 186 GAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLN 245
              +N++GSHG KEAILE+IKPWIQREL+AIL DPDPTIIVHLVTSLFV+RLEAPSSQLN
Sbjct: 186 CLVMNLTGSHGVKEAILERIKPWIQRELEAILGDPDPTIIVHLVTSLFVARLEAPSSQLN 245

Query: 246 PEDDFLLALHPFLYEKTDLFWHELR 270
            E+DFL  L PFLYEKTDLFWHELR
Sbjct: 246 AENDFLSPLRPFLYEKTDLFWHELR 270

[Close]

BLAST of MC07g0764 vs. ExPASy TrEMBL
Match: A0A1S3C7X8 (E3 ubiquitin-protein ligase Topors OS=Cucumis melo OX=3656 GN=LOC103497872 PE=4 SV=1)

HSP 1 Score: 410 bits (1053), Expect = 4.36e-142
Identity = 204/266 (76.69%), Postives = 224/266 (84.21%), Query Frame = 0


Query: 7   MKPA--TREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVECI 66
           MKP+  TREP RKLN ENF++KVISPAICGETCPICLREL+D T AVLT+CIHAYC+ CI
Sbjct: 1   MKPSSSTREPARKLNGENFITKVISPAICGETCPICLRELEDRTAAVLTTCIHAYCIGCI 60

Query: 67  RKWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLL 126
           RKWS LKRTCPLCNAQF+SWF+KIN SS SFRKE L T NC +KL  GVGSS+ D+R +L
Sbjct: 61  RKWSNLKRTCPLCNAQFDSWFTKINLSSQSFRKERLKTSNCSEKLKVGVGSSQTDARGIL 120

Query: 127 QRARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVR 186
           Q  R ELNR RRSE  LTWRRSFGRRG + +PADV+ +RKLQWRASIYNR IQAVPSSVR
Sbjct: 121 QSTRYELNRERRSERSLTWRRSFGRRGPDSLPADVVVRRKLQWRASIYNRCIQAVPSSVR 180

Query: 187 CGAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQL 246
              ELNI G+ G KE ILE+IKPWIQRELQ ILEDPDPTIIVHLV SLFV+RLEA SSQL
Sbjct: 181 SCLELNILGNRGGKEVILERIKPWIQRELQVILEDPDPTIIVHLVISLFVARLEATSSQL 240

Query: 247 NPEDDFLLALHPFLYEKTDLFWHELR 270
           N EDDFL  L PFL+EKTDLFWHELR
Sbjct: 241 NAEDDFLSPLRPFLFEKTDLFWHELR 266

[Close]

BLAST of MC07g0764 vs. ExPASy TrEMBL
Match: A0A0A0KCD1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G198260 PE=4 SV=1)

HSP 1 Score: 411 bits (1057), Expect = 6.24e-142
Identity = 204/268 (76.12%), Postives = 225/268 (83.96%), Query Frame = 0


Query: 5   EIMK--PATREPRRKLNRENFVSKVISPAICGETCPICLRELQDCTTAVLTSCIHAYCVE 64
           E+MK   +TREP RKLN ENF++KVISPAICGETCPICLREL+D T AVLT+CIHAYC+ 
Sbjct: 49  EVMKRSSSTREPARKLNGENFITKVISPAICGETCPICLRELEDRTAAVLTTCIHAYCIS 108

Query: 65  CIRKWSTLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRR 124
           CIRKWS LKRTCPLCNAQF+SWF+KIN SS SFRKE L T NC DKL  GVGSS+ID+R 
Sbjct: 109 CIRKWSNLKRTCPLCNAQFDSWFTKINLSSQSFRKERLTTSNCSDKLKVGVGSSQIDARG 168

Query: 125 LLQRARNELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSS 184
           +LQ  R ELNR RRSE  LTWRRSFGRRG + +PADV+ +RK QWRASIYNR IQAVPSS
Sbjct: 169 ILQSTRYELNRERRSERSLTWRRSFGRRGPDSLPADVVVRRKRQWRASIYNRCIQAVPSS 228

Query: 185 VRCGAELNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSS 244
           VR   ELN+ GS G KE ILE+IKPWIQRELQ ILEDPDPTIIVHLV SLFV+R+EA SS
Sbjct: 229 VRSCLELNVLGSRGGKEVILERIKPWIQRELQVILEDPDPTIIVHLVISLFVARIEATSS 288

Query: 245 QLNPEDDFLLALHPFLYEKTDLFWHELR 270
           QLN EDDFL  L PFL+EKTDLFWHELR
Sbjct: 289 QLNAEDDFLSPLRPFLFEKTDLFWHELR 316

[Close]

BLAST of MC07g0764 vs. TAIR 10
Match: AT2G39100.1 (RING/U-box superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.0e-58
Identity = 121/267 (45.32%), Postives = 170/267 (63.67%), Query Frame = 0


Query: 10  ATREPRRKLNRENFVSKVISPAICGETCPICLRELQD-CTTAVLTSCIHAYCVECIRKWS 69
           ++R  R   +   F  + I PA+ G++CPICL  L +  + AV+T C H YC+ CIRKWS
Sbjct: 12  SSRHLRSPESIAKFAGRAIFPALQGKSCPICLENLTERRSAAVITVCKHGYCLACIRKWS 71

Query: 70  TLKRTCPLCNAQFESWFSKINFSSSSFRKEHLPTLNCVDKLNTGVGSSRIDSRRLLQRAR 129
           + KR CPLCN +F+SWF   +F+S  + KE LP L   + L T   ++  D RR++QR+R
Sbjct: 72  SFKRNCPLCNTRFDSWFIVSDFASRKYHKEQLPILRDRETL-TYHRNNPSDRRRIIQRSR 131

Query: 130 NELNRVRRSEIPLTWRRSFGRRGSEPVPADVIAQRKLQWRASIYNRHIQAVPSSVRCGAE 189
           + L        PL WRRSFGR GS  VP  VI QRKLQWRASIY + ++AV    R   E
Sbjct: 132 DVLENSSSRSRPLPWRRSFGRPGS--VPDSVIFQRKLQWRASIYTKQLRAVRLHSR-RLE 191

Query: 190 LNISGSHGAKEAILEKIKPWIQRELQAILEDPDPTIIVHLVTSLFVSRLEAPSSQLNPE- 249
           L+++ +   K  I E+I+PWI+RELQA+L DPDP++IVH  ++LF+ RLE  +++   + 
Sbjct: 192 LSLAVNDYTKAKITERIEPWIRRELQAVLGDPDPSVIVHFASALFIKRLERENNRQTGQT 251

Query: 250 ----DDFLLALHPFLYEKTDLFWHELR 271
               +D + +L  FL +K D+FWHELR
Sbjct: 252 GMLVEDEVSSLRKFLSDKVDIFWHELR 274

[Close]

BLAST of MC07g0764 vs. TAIR 10
Match: AT4G10940.1 (RING/U-box protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.4e-06
Identity = 22/49 (44.90%), Postives = 28/49 (57.14%), Query Frame = 0


Query: 34 GETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKRTCPLCNAQFE 83
          GE C IC+  + D    VL  C H +C ECI  WST+   CPLC  +F+
Sbjct: 28 GERCGICMDIIID--RGVLDCCQHWFCFECIDNWSTIMNLCPLCQREFQ 74

[Close]

BLAST of MC07g0764 vs. TAIR 10
Match: AT5G19430.1 (RING/U-box superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 9.8e-06
Identity = 22/51 (43.14%), Postives = 29/51 (56.86%), Query Frame = 0


Query: 34 GETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKR--TCPLCNAQFE 83
          G  C ICL E+    TA++  C H YCV CI +W++ K   TCP C   F+
Sbjct: 43 GGCCAICLSEIPLQETAMVKGCEHTYCVTCILRWASCKESPTCPQCKHPFD 93

[Close]

BLAST of MC07g0764 vs. TAIR 10
Match: AT5G19430.2 (RING/U-box superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 9.8e-06
Identity = 22/51 (43.14%), Postives = 29/51 (56.86%), Query Frame = 0


Query: 34 GETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKR--TCPLCNAQFE 83
          G  C ICL E+    TA++  C H YCV CI +W++ K   TCP C   F+
Sbjct: 28 GGCCAICLSEIPLQETAMVKGCEHTYCVTCILRWASCKESPTCPQCKHPFD 78

[Close]

BLAST of MC07g0764 vs. TAIR 10
Match: AT5G12310.1 (RING/U-box superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.7e-05
Identity = 22/51 (43.14%), Postives = 29/51 (56.86%), Query Frame = 0


Query: 34 GETCPICLRELQDCTTAVLTSCIHAYCVECIRKWSTLKR--TCPLCNAQFE 83
          G  C ICL  +    TA++  C HAYCV CI +W++ K   TCP C   F+
Sbjct: 40 GGCCAICLDTIPLQETAMVKGCEHAYCVTCILRWASYKEKPTCPQCKLPFD 90

The following BLAST results are available for this feature:

BLAST of MC07g0764 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P08393	8.1e-05	36.17	E3 ubiquitin-protein ligase ICP0 OS=Human herpesvirus 1 (strain 17) OX=10299 GN=...	[more]
P29129	2.4e-04	36.84	E3 ubiquitin-protein ligase ICP0 OS=Suid herpesvirus 1 (strain Indiana-Funkhause...	[more]
P28284	3.1e-04	35.56	E3 ubiquitin-protein ligase ICP0 OS=Human herpesvirus 2 (strain HG52) OX=10315 G...	[more]
Q9P1Y6	3.1e-04	31.75	PHD and RING finger domain-containing protein 1 OS=Homo sapiens OX=9606 GN=PHRF1...	[more]
Q7ZWF4	4.0e-04	40.82	RING finger protein 145 OS=Danio rerio OX=7955 GN=rnf145 PE=2 SV=1	[more]

BLAST of MC07g0764 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138780.1	3.17e-192	100.00	E3 ubiquitin-protein ligase Topors [Momordica charantia]	[more]
XP_022999966.1	5.97e-148	77.78	E3 ubiquitin-protein ligase Topors isoform X1 [Cucurbita maxima]	[more]
XP_038875513.1	3.19e-147	78.15	E3 ubiquitin-protein ligase Topors isoform X2 [Benincasa hispida]	[more]
XP_038875512.1	4.89e-147	78.15	E3 ubiquitin-protein ligase Topors isoform X1 [Benincasa hispida]	[more]
KAG6593954.1	1.99e-146	78.87	E3 ubiquitin-protein ligase Topors, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of MC07g0764 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAG7	1.54e-192	100.00	E3 ubiquitin-protein ligase Topors OS=Momordica charantia OX=3673 GN=LOC11100985...	[more]
A0A6J1KEK6	2.89e-148	77.78	E3 ubiquitin-protein ligase Topors isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1EQ79	1.94e-146	78.87	E3 ubiquitin-protein ligase Topors isoform X1 OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A1S3C7X8	4.36e-142	76.69	E3 ubiquitin-protein ligase Topors OS=Cucumis melo OX=3656 GN=LOC103497872 PE=4 ...	[more]
A0A0A0KCD1	6.24e-142	76.12	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G198260 P...	[more]

BLAST of MC07g0764 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39100.1	1.0e-58	45.32	RING/U-box superfamily protein	[more]
AT4G10940.1	3.4e-06	44.90	RING/U-box protein	[more]
AT5G19430.1	9.8e-06	43.14	RING/U-box superfamily protein	[more]
AT5G19430.2	9.8e-06	43.14	RING/U-box superfamily protein	[more]
AT5G12310.1	1.7e-05	43.14	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 37..77 e-value: 5.0E-5 score: 32.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 36..78 e-value: 3.9E-8 score: 33.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 37..78 score: 12.946621
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 34..133 e-value: 1.3E-12 score: 50.0
None	No IPR available	PANTHER	PTHR46077	E3 UBIQUITIN-PROTEIN LIGASE TOPORS	coord: 12..272
None	No IPR available	PANTHER	PTHR46077:SF1	TOPOISOMERASE I-BINDING, ARGININE/SERINE-RICH A-RELATED	coord: 12..272
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 31..85
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 55..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0764.1	MC07g0764.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006513	protein monoubiquitination
biological_process	GO:0000209	protein polyubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity