MC07g0650 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0650
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pyruvate kinase
Location	MC07: 14412476 .. 14417190 (-)
RNA-Seq Expression	MC07g0650
Synteny	MC07g0650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCAAAATTGCTCTATAACCAATCAATAAATTAAATAAAATTCAACCAAAACATATAAATCCAAATCATGTTACAATAACCTGGCCACAAAATAAAGTAAATAAAAATTACTCTAATAAAAATAATCATATAATACCATTGGAAGAATGAGTTAAAATTGACTCACGTGGGATAAAATTTGAAAATTTCGTTCAATTTATTCTAAAAAAAAAAAAACTGTCAACATATTTTTATCGTGCGTAAAATAACAAATTATTGAAGCCAATCGTCACCGTCCACGTCACACTATAAAACCCGCAATAAACCTCATCCCATCCGATCCGATCCTTTTTGAGTCCCATCTCTAAGCCGTTCTCCGCCGTCGATCTCCCGGCGGCAATTCCAAAGCTTCCATCGCCAAAAATTTCCACCGTCTTCGTCATCTCCGTCCTCTCTCTGCTATAATTCTCCCCTTCAATTCCCATTTCTCTCTTCAATTCGCAATGGCGCAGTCTCTGCAACTCTTCACCACCACCACCCGCTCTCCCACCCTCTCCCTGACCAAAAATTCGCTTCCAAAATCCGCCCCTTCCACCGCCGCTTTCCGCTTCCCCTTCACTCTCACCAAATCGTCAATTCGAGCCTCTTCCTCTCCGGATCTCAATCCTCTACCGGCGTCCTCCACTTCCCAAGTCTTGGTTTCCGAAAATGGCTCGAGCGGCGGAGGTGGGTATGCGCCGGTTGTGTCTGATGCGAGCTCTATTGAGGTCGACGCTGTGACGGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGTACGAGGAGGACGAAGCTTGTGTGTACTATTGGCCCTGCTACTTGCGGATTCGAGCAGCTGGAAGCGCTCGCTGTTGGCGGTATGAATGTGGCGAGAATCAATATGTGCCATGGAACTCGGGAGTGGCATCGGGATGTGATTGAACGCGTGCGGAGGCTCAATGAGGAGAAGGGCTATGCAGTGGCCATCATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGACCTTGGAGGAGCTTCTTCAGCTAAAGCGGAAGTGAGTTTTGTTCTTTCATCGTTTTCCATCTTTCTGTAGAGGACAATTGGTGAAACTTTTGCTGGTCTGTGTCCTAATTTTAATATTTTATATCTGAATTGTCTTTTTAGAACTTTTTGAGTACTGGGTTTTATCTGGCTTACCAACAGGCGAGCTGAATAAGATGAATGTGATTTTCAACTTTCTTCTGCCTGAAACTCAAAAATTCGCCCAATTTAATATTTTTATCATTCTTAAACTGGATCGGGCTTCACCTCGGACGGAGGCATTTGTATTACCCATTTAAATTTTATGTTGTTTTAAAAGCAAGATGGGTCCTTTTACGTTGTTTTAAAAGCAAGATGAGAGAGAATGGTGAAATCTGGACATTCAGCAATAAGTAGTTTATTGCAGCAATAAATCCTCTTAATGATGGTCTCACATTTCTGCTATCTTACATCTGACTTCAGTTTAAGTAGGCCGACTGAAGCCGTTGGGACGTGTATAACTGCTTGCTCTGTTCTATGTAGGATGGTGAAATCTGGACATTCAGCGTCAGAGCTTTCGATTCAACACTGCCAGTCCCAGAACGCACTATTAATGTGAACTATGAGGGGTTTGCAGAAGGTTACTAATTCTATCCTAAATTAAAAGTTTTCTCTGGCATTTACTGTATTCGTGCAGAATCTAAGATATGGTTCTGGGAATTGGCATATGTGGTCGGATCTGCGTTCATAATTGTAGTAAATTCTATGAAATTGTTGTTAATCTTGAATTATAACTGATATTGTGTAGATGTGAGAGTGGGTGATGACCTCCTTGTTGACGGTGGAATGGTGAGATTTGAGGTAATCGAAAAGATTGGGCCGGATGTTAAGTGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTGGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGTAGGTGCCTCCTTGCTCCTTGAACTGTCATTTCCTTAGGTCAACCTAAACAATTTGTTTTAGCTCACTGGCTTACTAATTGCCGTTGTGCATAACCAACACCAGAGAGTTACAGACTACAAGAACTTGAATCATCTAGTCTTGCATATTTTCTTCAACCCGTGCATCGCTAATTTTAGATTACTGTTTTAGAAAATGTTAACTTATTTTTTATTATGTTAGAAATGAAAAGATCGTTACTGTCCAATCATAATGAAACACTATTGTTTTAATTGGCTTAGATATTAAACTTTTAATTTTGGCATTGCCAGAAACTCGAGTGTTTTCTATTTACACTTGGCTGAGTAACTAATTTTATGTTGAAAGATCATGTCGAGAGTATTATAATTTGCACCATGAATTCTAGAAGGAAATGTTTGATGGGAGAGTAGTAGTTATATCATACTGAAAACCTTTCATGTAACCTCATTATTGCATCTGTTTCTGGATCCTAGTCTCATGATGTTTGAAGTTAAATCTTTCGTGCAACTTAGCGATGCAATTTACCCTGATACAGTGACTTAATTTCTAGTCTAATGTGCATAATTTATGAATTCAGGATTGGTTGGATATTGATTTTGGGATTGCAGAAGGCGTTGATTTTCTTGCCATTTCATTTGTCAAGTCCGCTGAAGTGATTAAACACCTCAAAAGCTATATTGCTGCACGTTCCCGTGGCAGGTATGAAGAAATTTAGTAATGTGCTGATCTGTTCTTCTTTGATTCTATTTGGGTCTGAAGCTATGAACCTCTCATTGTTGGTTTTCACGTTTGTTGGGAGATCAAATACATCTCAAGTATGTATTAGTATCCTCTGATTCCCAAGTTAGATGTATTTCTTTTGAGTTTCTTGGTTGTTGTTGACTTTTAATAAATTTTTAAAATCAGTTTTCAATTTGGACAGGGTGGTTGACGAACAAATTATCTCTCTCCCTCTCTAATGCTTCGAACTTTATCTTAAATTCTGTTATATGTACACCTTTATATTTATGTCTCTTTTTGTTTTCCATGGTATGCAGTGACATTTCCATCATTGCGAAGATAGAGAGTATGGACTCATTGAAGAACTTGGAAGAAATTATTCGAGCATCGGATGGGGCTATGGTAGCTAGAGGAGATCTAGGAGCTCAGATTCCACTGGAACAGGTGCCATCAGTCCAGCAAAAGATCGTCCAACTATGCAGGCAGTTAAATAAGCCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCGATGATCGAATATCCAACACCCACCAGAGCTGAAGTTGCCGATGTTTCCGAGGCTGTCAGGCAGCGATCAGATGCCCTGATGCTCTCTGGTGAGTCCGCCATGGGCCAGTTCCCAGACAAGGCATTAGCTGTTTTGAGAAGTGTCAGTCTAAGGATTGAGAGGTGGTGGAGGGAAGAGAAACACCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTCAGAAGAGATCTGCAATTCAGCTGCCAAAATGGGTAAGCTTTATCATATCCCTGGATATCTAAAGTGGTTACAAAGCTATATTCTCACTAGCTATAGGAGAAATTCGTGGAGAAGGCTGGAATTGATAGTTTTACACTGTTGGATGCATGAAAGAAATTACACCATGTATTTTGATCTGTATCTCATGGTTATTAGGGGCATATAAATTTTGCATCTGCATCCACTAGTTATTTAGAGGGTAGCTATAGCAACCTCATTCAATTATTTGTCCAGCTATATGCTTTAGTACCATGAAGAACACAACCAGTATCTCATATCATTTCACAAAAATGGTTATTGCTTTGTAAAGATGCAGCATGGAAGATAGTTAGTGGAGTTGGGTCTCTCGATATGCTGCGGTCTTCTTTATAGTAGTTTCCTCTTGTTGTTGCTTATTGCAACTAATTTTGAGCTTTGTTTCCGTAGCCAATAATTTGGAGGTGGATGCAATTTTCGTCTACACAAGGAAAGGCCACATGGCATCTCTCCTGTCCCGCTGCCGACCCGATTGCCCGATCTTTGCATTTACCTCCACAACATCCGTGAGGCGACGTCTTAACTTGCAATGGGGCTTGATTCCCTTCCGCCTGAGCTTCTCTGAGGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGATCTTGTAATTGCTGTCTCAGACATGTTGCAGTCTATCCAGGTTATGAATGTGCCTTGAGAAAGATAGGCTGATCTGTGTTGCCTGCTGCTGTTTTTTCATTTCTTTAGATTATTTAACTTTATCAAAGCTTCTGGATCTGTTATTTAGTAGCTAGGAAAAGAGATCAAAACACTAGCTTCAGTTTTTTCTCCTATCTTTGCCTCTGAGATTTCCTGAATGTCATCTGAAACGTCGAGTTGAACTCGAGTTTTTTTGCTTGATTTCTTTTTATTATCTGTAACATTTTAATGACTATCAGATAAATGTTCTATGAACAATCATGGTCTAGGAACTATGTATGTAATTTTCGGGATAGCATTATGTTATACCATGTATTTTAGCTTTGAATGAATTGAATTTTGGGGTTGATATGAAGCATCTTGGGGACGTGGAACTTGCCTAAAACCATGATCATTTTACTACTTTATATAATTGATGAAC

mRNA sequence

CTCAAAATTGCTCTATAACCAATCAATAAATTAAATAAAATTCAACCAAAACATATAAATCCAAATCATGTTACAATAACCTGGCCACAAAATAAAGTAAATAAAAATTACTCTAATAAAAATAATCATATAATACCATTGGAAGAATGAGTTAAAATTGACTCACGTGGGATAAAATTTGAAAATTTCGTTCAATTTATTCTAAAAAAAAAAAAACTGTCAACATATTTTTATCGTGCGTAAAATAACAAATTATTGAAGCCAATCGTCACCGTCCACGTCACACTATAAAACCCGCAATAAACCTCATCCCATCCGATCCGATCCTTTTTGAGTCCCATCTCTAAGCCGTTCTCCGCCGTCGATCTCCCGGCGGCAATTCCAAAGCTTCCATCGCCAAAAATTTCCACCGTCTTCGTCATCTCCGTCCTCTCTCTGCTATAATTCTCCCCTTCAATTCCCATTTCTCTCTTCAATTCGCAATGGCGCAGTCTCTGCAACTCTTCACCACCACCACCCGCTCTCCCACCCTCTCCCTGACCAAAAATTCGCTTCCAAAATCCGCCCCTTCCACCGCCGCTTTCCGCTTCCCCTTCACTCTCACCAAATCGTCAATTCGAGCCTCTTCCTCTCCGGATCTCAATCCTCTACCGGCGTCCTCCACTTCCCAAGTCTTGGTTTCCGAAAATGGCTCGAGCGGCGGAGGTGGGTATGCGCCGGTTGTGTCTGATGCGAGCTCTATTGAGGTCGACGCTGTGACGGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGTACGAGGAGGACGAAGCTTGTGTGTACTATTGGCCCTGCTACTTGCGGATTCGAGCAGCTGGAAGCGCTCGCTGTTGGCGGTATGAATGTGGCGAGAATCAATATGTGCCATGGAACTCGGGAGTGGCATCGGGATGTGATTGAACGCGTGCGGAGGCTCAATGAGGAGAAGGGCTATGCAGTGGCCATCATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGACCTTGGAGGAGCTTCTTCAGCTAAAGCGGAAGATGGTGAAATCTGGACATTCAGCGTCAGAGCTTTCGATTCAACACTGCCAGTCCCAGAACGCACTATTAATGTGAACTATGAGGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTCCTTGTTGACGGTGGAATGGTGAGATTTGAGGTAATCGAAAAGATTGGGCCGGATGTTAAGTGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTGGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCAGAAGGCGTTGATTTTCTTGCCATTTCATTTGTCAAGTCCGCTGAAGTGATTAAACACCTCAAAAGCTATATTGCTGCACGTTCCCGTGGCAGTGACATTTCCATCATTGCGAAGATAGAGAGTATGGACTCATTGAAGAACTTGGAAGAAATTATTCGAGCATCGGATGGGGCTATGGTAGCTAGAGGAGATCTAGGAGCTCAGATTCCACTGGAACAGGTGCCATCAGTCCAGCAAAAGATCGTCCAACTATGCAGGCAGTTAAATAAGCCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCGATGATCGAATATCCAACACCCACCAGAGCTGAAGTTGCCGATGTTTCCGAGGCTGTCAGGCAGCGATCAGATGCCCTGATGCTCTCTGGTGAGTCCGCCATGGGCCAGTTCCCAGACAAGGCATTAGCTGTTTTGAGAAGTGTCAGTCTAAGGATTGAGAGGTGGTGGAGGGAAGAGAAACACCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTCAGAAGAGATCTGCAATTCAGCTGCCAAAATGGCCAATAATTTGGAGGTGGATGCAATTTTCGTCTACACAAGGAAAGGCCACATGGCATCTCTCCTGTCCCGCTGCCGACCCGATTGCCCGATCTTTGCATTTACCTCCACAACATCCGTGAGGCGACGTCTTAACTTGCAATGGGGCTTGATTCCCTTCCGCCTGAGCTTCTCTGAGGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGATCTTGTAATTGCTGTCTCAGACATGTTGCAGTCTATCCAGGTTATGAATGTGCCTTGAGAAAGATAGGCTGATCTGTGTTGCCTGCTGCTGTTTTTTCATTTCTTTAGATTATTTAACTTTATCAAAGCTTCTGGATCTGTTATTTAGTAGCTAGGAAAAGAGATCAAAACACTAGCTTCAGTTTTTTCTCCTATCTTTGCCTCTGAGATTTCCTGAATGTCATCTGAAACGTCGAGTTGAACTCGAGTTTTTTTGCTTGATTTCTTTTTATTATCTGTAACATTTTAATGACTATCAGATAAATGTTCTATGAACAATCATGGTCTAGGAACTATGTATGTAATTTTCGGGATAGCATTATGTTATACCATGTATTTTAGCTTTGAATGAATTGAATTTTGGGGTTGATATGAAGCATCTTGGGGACGTGGAACTTGCCTAAAACCATGATCATTTTACTACTTTATATAATTGATGAAC

Coding sequence (CDS)

ATGGCGCAGTCTCTGCAACTCTTCACCACCACCACCCGCTCTCCCACCCTCTCCCTGACCAAAAATTCGCTTCCAAAATCCGCCCCTTCCACCGCCGCTTTCCGCTTCCCCTTCACTCTCACCAAATCGTCAATTCGAGCCTCTTCCTCTCCGGATCTCAATCCTCTACCGGCGTCCTCCACTTCCCAAGTCTTGGTTTCCGAAAATGGCTCGAGCGGCGGAGGTGGGTATGCGCCGGTTGTGTCTGATGCGAGCTCTATTGAGGTCGACGCTGTGACGGAGGCTGAGTTGAAGGAGAATGGCTTCAGGAGTACGAGGAGGACGAAGCTTGTGTGTACTATTGGCCCTGCTACTTGCGGATTCGAGCAGCTGGAAGCGCTCGCTGTTGGCGGTATGAATGTGGCGAGAATCAATATGTGCCATGGAACTCGGGAGTGGCATCGGGATGTGATTGAACGCGTGCGGAGGCTCAATGAGGAGAAGGGCTATGCAGTGGCCATCATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGACCTTGGAGGAGCTTCTTCAGCTAAAGCGGAAGATGGTGAAATCTGGACATTCAGCGTCAGAGCTTTCGATTCAACACTGCCAGTCCCAGAACGCACTATTAATGTGAACTATGAGGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTCCTTGTTGACGGTGGAATGGTGAGATTTGAGGTAATCGAAAAGATTGGGCCGGATGTTAAGTGCCTGTGTACTGACCCGGGATTGTTGTTGCCACGGGCTAATTTGACTTTTTGGAGGGATGGACATCTGGTACGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGGATTGCAGAAGGCGTTGATTTTCTTGCCATTTCATTTGTCAAGTCCGCTGAAGTGATTAAACACCTCAAAAGCTATATTGCTGCACGTTCCCGTGGCAGTGACATTTCCATCATTGCGAAGATAGAGAGTATGGACTCATTGAAGAACTTGGAAGAAATTATTCGAGCATCGGATGGGGCTATGGTAGCTAGAGGAGATCTAGGAGCTCAGATTCCACTGGAACAGGTGCCATCAGTCCAGCAAAAGATCGTCCAACTATGCAGGCAGTTAAATAAGCCAGTTATTGTTGCTTCTCAGCTGCTTGAGTCGATGATCGAATATCCAACACCCACCAGAGCTGAAGTTGCCGATGTTTCCGAGGCTGTCAGGCAGCGATCAGATGCCCTGATGCTCTCTGGTGAGTCCGCCATGGGCCAGTTCCCAGACAAGGCATTAGCTGTTTTGAGAAGTGTCAGTCTAAGGATTGAGAGGTGGTGGAGGGAAGAGAAACACCATGAACCTATGGAACTCCCAGAAGTTGGATCTTCATTCTCAGATAGTATCTCAGAAGAGATCTGCAATTCAGCTGCCAAAATGGCCAATAATTTGGAGGTGGATGCAATTTTCGTCTACACAAGGAAAGGCCACATGGCATCTCTCCTGTCCCGCTGCCGACCCGATTGCCCGATCTTTGCATTTACCTCCACAACATCCGTGAGGCGACGTCTTAACTTGCAATGGGGCTTGATTCCCTTCCGCCTGAGCTTCTCTGAGGACATGGAAAACAACCTCAACAAAACATTTTTGCTGCTTAAAGCCAGAAACTTGATCAAATCAGGTGATCTTGTAATTGCTGTCTCAGACATGTTGCAGTCTATCCAGGTTATGAATGTGCCTTGA

Protein sequence

MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASSTSQVLVSENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP

Homology

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BLAST of MC07g0650 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 5.9e-271
Identity = 493/596 (82.72%), Postives = 531/596 (89.09%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPK-SAP-STAAFRFPFTLTKS-SIRASSSPDLNPLP 60
           M+QSL        SP L+  K   PK   P  T+  R+P    KS SI+AS+SP      
Sbjct: 1   MSQSLHF------SPNLTFAKQPFPKLPLPFPTSNSRYPVNNYKSLSIKASTSPS----- 60

Query: 61  ASSTSQVLVSENGSSGGG------GYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLV 120
           +SS  QVLV++NG+   G        +  VSD SSIEVDAVTE ELKENGFRSTRRTKLV
Sbjct: 61  SSSDPQVLVADNGTGNSGVLYNNNNKSVTVSDPSSIEVDAVTETELKENGFRSTRRTKLV 120

Query: 121 CTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPATCGFE+LEALAVGGMNVARINMCHGTREWH+ VIERVRRLNEEKG+AVAIMMDT
Sbjct: 121 CTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEEKGFAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLL 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRA+DS  P PERTINVNY+GFAEDV+VGD+LL
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS--PRPERTINVNYDGFAEDVKVGDELL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKC CTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRA 360
           IDFGIAEGVDF+AISFVKSAEVI HLKSYIAARSR SDI++IAKIES+DSLKNLEEIIRA
Sbjct: 301 IDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLEEIIRA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPS QQ IVQ+CRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSS 480
           DVSEAVRQR+DALMLSGESAMGQ+P+KALAVLRSVS+RIE+WWREEKHHE MELP +GS+
Sbjct: 421 DVSEAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELPAIGST 480

Query: 481 FSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540
           +SDSISEEICNSAAKMANNL VDA+FVYT+ GHMASLLSRCRPDCPIFAFT+TTSVRRRL
Sbjct: 481 YSDSISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTTSVRRRL 540

Query: 541 NLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 588
           NLQWGLIPFRLSF++DME+NLNKTF LLKAR +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSIQVMNVP 583

[Close]

BLAST of MC07g0650 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 1.8e-264
Identity = 482/601 (80.20%), Postives = 529/601 (88.02%), Query Frame = 0


Query: 1   MAQSLQLF-TTTTRSP-TLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRA----SSSPDLN 60
           M+Q+L  F ++++RSP T ++++   P   PST + R    L K S+R     +SS   +
Sbjct: 1   MSQALNFFVSSSSRSPATFTISR---PSVFPSTGSLRL---LVKKSLRTLVVEASSAAAS 60

Query: 61  PLPASSTSQVLVSENGSSG--------GGGYAPVVSDASSIEVDAVTEAELKENGFRSTR 120
            L    +S VLVSENGS G         G  A   +D+SSIEVD VTEAELKENGFRSTR
Sbjct: 61  DLDEPQSSPVLVSENGSGGVLSSATQEYGRNAAPGTDSSSIEVDTVTEAELKENGFRSTR 120

Query: 121 RTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVA 180
           RTKL+CTIGPATCGFEQLE LA GGMNVARINMCHGTREWHR VIER+RRLNEEKG+AVA
Sbjct: 121 RTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTREWHRMVIERLRRLNEEKGFAVA 180

Query: 181 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRV 240
           IMMDTEGSEIHMGDLGGASSAKAEDGEIW F+VR+FD   P+PERT+ VNY+GFAEDV+V
Sbjct: 181 IMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP--PLPERTVTVNYDGFAEDVKV 240

Query: 241 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 300
           GD+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISS
Sbjct: 241 GDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGKLVRERNAMLPTISS 300

Query: 301 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLE 360
           KDWLDIDFGIAEGVDF+A+SFVKSAEVIKHLKSYI AR+R SDIS+IAKIES+DSLKNLE
Sbjct: 301 KDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARARDSDISVIAKIESIDSLKNLE 360

Query: 361 EIIRASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPT 420
           EII+ASDGAMVARGDLGAQIPLEQVPS QQKIVQ+CRQLN+PVIVASQLLESMIEYP PT
Sbjct: 361 EIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLNRPVIVASQLLESMIEYPIPT 420

Query: 421 RAEVADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELP 480
           RAEVADVSEAVRQR DALMLSGESAMGQFP+KAL VLRSVSLRIER WRE+K HE +ELP
Sbjct: 421 RAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSVSLRIERMWREQKRHEVIELP 480

Query: 481 EVGSSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTS 540
            + SSFSDSISEEICNSAAKMANNLEVDA+FVYT+ GHMASLLSRCRPDCPIFAFT+TTS
Sbjct: 481 SIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMASLLSRCRPDCPIFAFTTTTS 540

Query: 541 VRRRLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNV 588
           VRRRLNLQWGL+PFRLSFS+DME+NLNKTF LLKAR +IKSGDL+IAVSDMLQSIQVMNV
Sbjct: 541 VRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIKSGDLIIAVSDMLQSIQVMNV 593

[Close]

BLAST of MC07g0650 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 7.0e-264
Identity = 477/598 (79.77%), Postives = 521/598 (87.12%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTK-SSIRASS----SPDLNP 60
           M+QS+Q  T +     L L  +   +   S +  RFP T  K +SIRASS    SPDL+ 
Sbjct: 1   MSQSIQFSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLDS 60

Query: 61  LPASSTSQVLVSENGSSGGGG------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTK 120
             +SS+SQVL+S NG+              V +D S IEVD VTEAELKENGFRSTRRTK
Sbjct: 61  SSSSSSSQVLLSPNGTGAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRSTRRTK 120

Query: 121 LVCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMM 180
           L+CTIGPATCGFEQLEALAVGGMNVAR+NMCHGTR+WHR VI  VRRLNEEKG+AVAIMM
Sbjct: 121 LICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVAIMM 180

Query: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDD 240
           DTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+   PERTI+V+Y+GFAEDVRVGD+
Sbjct: 181 DTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSR--PERTISVSYDGFAEDVRVGDE 240

Query: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW 300
           LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDW
Sbjct: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDW 300

Query: 301 LDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEII 360
           LDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEEII
Sbjct: 301 LDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEII 360

Query: 361 RASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAE 420
            ASDGAMVARGDLGAQIPLEQVP+ QQ+IVQ+CR LNKPVIVASQLLESMIEYPTPTRAE
Sbjct: 361 LASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRAE 420

Query: 421 VADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVG 480
           VADVSEAVRQRSDALMLSGESAMGQFPDKAL VLR+VSLRIERWWREEK HE + L  +G
Sbjct: 421 VADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAIG 480

Query: 481 SSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRR 540
           SSFSD ISEEICNSAAKMANNL VDA+FVYT  GHMASL+SRCRPDCPIFAFT+TTSVRR
Sbjct: 481 SSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSVRR 540

Query: 541 RLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 588
           RLNLQWGLIPFRLSFS+DME+NLNKTF LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

[Close]

BLAST of MC07g0650 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.8e-101
Identity = 211/479 (44.05%), Postives = 297/479 (62.00%), Query Frame = 0


Query: 104 STRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEE-KG 163
           S R+TK+VCTIGP+T   E +  LA  GMNVAR+NM HG    H+  I+ V+  N + + 
Sbjct: 89  SPRKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFED 148

Query: 164 YAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAE 223
             +AIM+DT+G E+  GD+        ++G+ + FS++   ST    E T++VNY+ F  
Sbjct: 149 KVIAIMLDTKGPEVISGDV--PKPILLKEGQEFNFSIKRGVST----EDTVSVNYDDFIN 208

Query: 224 DVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLP 283
           DV  GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP
Sbjct: 209 DVEAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLN-------VRGKSATLP 268

Query: 284 TISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSL 343
           +I+ KDW DI FG+   VDF A+SFVK A+V+  LK Y+  +S  +DI +I KIES DS+
Sbjct: 269 SITEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYL--KSCNADIHVIVKIESADSI 328

Query: 344 KNLEEIIRASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEY 403
            NL  II ASDGAMVARGDLGA++P+E+VP +Q+ I++ C+ + KPVIVA+ +LESMI++
Sbjct: 329 PNLHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDH 388

Query: 404 PTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEP 463
           PTPTRAEV+D+S AVR+ +DA+MLSGE+A G++P KA+ V+  V+LR      E    + 
Sbjct: 389 PTPTRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALR-----TESSLQKS 448

Query: 464 MELPEVGSSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFT 523
              P   +++   + E     ++ MAN L    I V+TR G MA +LS  RP   +FAFT
Sbjct: 449 TSSPSQSAAYKSHMGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFT 508

Query: 524 STTSVRRRLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 582
           +   V++RL L  G++P  + FS D E   ++   LL +++L+K G  V  V    Q I
Sbjct: 509 NNERVKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPI 546

[Close]

BLAST of MC07g0650 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 2.9e-100
Identity = 210/477 (44.03%), Postives = 293/477 (61.43%), Query Frame = 0


Query: 106 RRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLN-EEKGYA 165
           R+TK+VCT+GP+T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 166 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDV 225
           +AIM+DT+G E+  GDL        + G+ +TF++    ST       ++VNY+ F  DV
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST----PSCVSVNYDDFVNDV 229

Query: 226 RVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTI 285
             GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I
Sbjct: 230 EAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSI 289

Query: 286 SSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKN 345
           + KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ N
Sbjct: 290 TEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPN 349

Query: 346 LEEIIRASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPT 405
           L  II ASDGAMVARGDLGA++P+E+VP +Q++I+ LCR + K VIVA+ +LESMI +PT
Sbjct: 350 LHSIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPT 409

Query: 406 PTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPME 465
           PTRAEV+D++ AVR+ +DA+MLSGE+A G+FP KA  V+ +V+LR E      +      
Sbjct: 410 PTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP---- 469

Query: 466 LPEVGSSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTST 525
            P +G +F + +SE     A  M+N L    + V+TR G MA LLS  RP   I+AFT+ 
Sbjct: 470 -PNLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNE 529

Query: 526 TSVRRRLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 582
             +++RL L  G+ P  + F++D E         L  + ++K G+ +  V    Q I
Sbjct: 530 KKIQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

[Close]

BLAST of MC07g0650 vs. NCBI nr
Match: XP_022138517.1 (pyruvate kinase isozyme A, chloroplastic [Momordica charantia])

HSP 1 Score: 1134 bits (2933), Expect = 0.0
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS
Sbjct: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60

Query: 61  TSQVLVSENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCG 120
           TSQVLVSENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCG
Sbjct: 61  TSQVLVSENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCG 120

Query: 121 FEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGD 180
           FEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGD
Sbjct: 121 FEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGD 180

Query: 181 LGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFE 240
           LGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFE
Sbjct: 181 LGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFE 240

Query: 241 VIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGV 300
           VIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGV
Sbjct: 241 VIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGV 300

Query: 301 DFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARG 360
           DFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARG
Sbjct: 301 DFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARG 360

Query: 361 DLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQR 420
           DLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQR
Sbjct: 361 DLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQR 420

Query: 421 SDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSSFSDSISEEI 480
           SDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSSFSDSISEEI
Sbjct: 421 SDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSSFSDSISEEI 480

Query: 481 CNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPF 540
           CNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPF
Sbjct: 481 CNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPF 540

Query: 541 RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587

[Close]

BLAST of MC07g0650 vs. NCBI nr
Match: XP_038874381.1 (pyruvate kinase isozyme A, chloroplastic [Benincasa hispida])

HSP 1 Score: 1037 bits (2681), Expect = 0.0
Identity = 540/596 (90.60%), Postives = 564/596 (94.63%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQLFT++TRSP +SL + SL K + S+AAFRFPF+ +KSSIRASSSPDLNPL +SS
Sbjct: 1   MTQSLQLFTSSTRSPGISLPRRSLSKPSSSSAAFRFPFSFSKSSIRASSSPDLNPLSSSS 60

Query: 61  TSQVLVSENGSSGGGG---------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGSS G G         +  + SD++SI+VDAVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSSSGTGVVSSSATKEFVSLASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPA+CGFEQLEALAVGGMNVARINMCHGTREWHR VIE VRRLN+EKGYAVAIMMDT
Sbjct: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTREWHRTVIEYVRRLNDEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLL 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDLL
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRA 360
           IDFGIAEGVDF+AISFVKSAEVIKHLKSYIAARSRGSDISIIAKIES+DSLKNLEEII A
Sbjct: 301 IDFGIAEGVDFIAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIVA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPSVQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSS 480
           DVSEAVRQRSDALMLSGESAMGQ+PDKALAVLRSVSLRIE+WWR+EK HEPMELPEVGSS
Sbjct: 421 DVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSS 480

Query: 481 FSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540
           FSD+I EEICNSAAKMANNLEVDAIFVYT  GHMASLLSRCRPDCPIFAFTSTTSVRRRL
Sbjct: 481 FSDTILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540

Query: 541 NLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           NLQWGLIPFRLSFS+DMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

[Close]

BLAST of MC07g0650 vs. NCBI nr
Match: XP_023006710.1 (pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 536/596 (89.93%), Postives = 562/596 (94.30%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQ+FT+ TRSP +SL+K+SL K +PSTAAFRFP + TKSSIRASSSPD NPL ++ 
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSGGGG---------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+GGGG         + PV+SD+++IEVD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDT 180
           CTIGP TCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLL 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDLL
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRA 360
           IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIES+DSL NLEEII A
Sbjct: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVP VQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSS 480
           D+SEAVRQRSDALMLSGESAMGQ+PDKALAVLR+VSLRIE+WWREEK HEPMELPEVGSS
Sbjct: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480

Query: 481 FSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540
            SDSI EEICNSAAKMANNLEVDAIFVYT+ GHMASLLSRCRPDCPIFAFT TTSVRRRL
Sbjct: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540

Query: 541 NLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           NLQWGLIPFR+SFS+DMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

[Close]

BLAST of MC07g0650 vs. NCBI nr
Match: XP_023548664.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1035 bits (2675), Expect = 0.0
Identity = 538/596 (90.27%), Postives = 561/596 (94.13%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQLFT+ TRSP +SL+K+SL K +PSTAAFRFP + TKSSIRASSSPD NPL ++ 
Sbjct: 1   MTQSLQLFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSGGGG---------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+ GGG         + PV+SD+++IEVD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTVGGGAVSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDT 180
           CTIGP TCGFEQLEALAVGGMNVARINMCHGTR WHR VIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRNWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLL 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDLL
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRA 360
           IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIES+DSL NLEEII A
Sbjct: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLNNLEEIIVA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPSVQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSS 480
           D+SEAVRQRSDALMLSGESAMGQ+PDKALAVLR+VSLRIE+WWREEK HEPMELPEVGSS
Sbjct: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480

Query: 481 FSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540
            SDSI EEICNSAAKMANNLEVDAIFVYT+ GHMASLLSRCRPDCPIFAFT TTSVRRRL
Sbjct: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540

Query: 541 NLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           NLQWGLIPFR+SFS+DMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

[Close]

BLAST of MC07g0650 vs. NCBI nr
Match: XP_004150564.1 (plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypothetical protein Csa_006584 [Cucumis sativus])

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 541/597 (90.62%), Postives = 560/597 (93.80%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQLFT    S  +SL K+S+ K + S+AAFRFPF+ +KSSIRASSS DLNPL +SS
Sbjct: 1   MTQSLQLFT----SSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSS 60

Query: 61  TSQVLVSENGSSGGGG----------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKL 120
           TSQVLVSENGSS GGG          +  V SD++SIEVDAVTEAELKENGFRSTRRTKL
Sbjct: 61  TSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKL 120

Query: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMD 180
           VCTIGPATCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+ KGYAVAIMMD
Sbjct: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDL 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDL
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIR 360
           DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIES+DSLKNLEEII 
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGS 480
           ADVSEAVRQRSDALMLSGESAMGQ+PDKALAVLRSVSLRIE+WWR+EK HEPMELPEVGS
Sbjct: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGS 480

Query: 481 SFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRR 540
           SFSDSI EEICNSAAKMANNLEVDAIFVYT  GHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 541 LNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           LNLQWGLIPFRLSFS+DMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591

[Close]

BLAST of MC07g0650 vs. ExPASy TrEMBL
Match: A0A6J1CBB4 (Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111009665 PE=3 SV=1)

HSP 1 Score: 1134 bits (2933), Expect = 0.0
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS
Sbjct: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60

Query: 61  TSQVLVSENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCG 120
           TSQVLVSENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCG
Sbjct: 61  TSQVLVSENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCG 120

Query: 121 FEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGD 180
           FEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGD
Sbjct: 121 FEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGD 180

Query: 181 LGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFE 240
           LGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFE
Sbjct: 181 LGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFE 240

Query: 241 VIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGV 300
           VIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGV
Sbjct: 241 VIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGV 300

Query: 301 DFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARG 360
           DFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARG
Sbjct: 301 DFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARG 360

Query: 361 DLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQR 420
           DLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQR
Sbjct: 361 DLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQR 420

Query: 421 SDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSSFSDSISEEI 480
           SDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSSFSDSISEEI
Sbjct: 421 SDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSSFSDSISEEI 480

Query: 481 CNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPF 540
           CNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPF
Sbjct: 481 CNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPF 540

Query: 541 RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587

[Close]

BLAST of MC07g0650 vs. ExPASy TrEMBL
Match: A0A6J1L0W9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1)

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 536/596 (89.93%), Postives = 562/596 (94.30%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQ+FT+ TRSP +SL+K+SL K +PSTAAFRFP + TKSSIRASSSPD NPL ++ 
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSGGGG---------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+GGGG         + PV+SD+++IEVD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDT 180
           CTIGP TCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLL 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDLL
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRA 360
           IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIES+DSL NLEEII A
Sbjct: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVP VQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSS 480
           D+SEAVRQRSDALMLSGESAMGQ+PDKALAVLR+VSLRIE+WWREEK HEPMELPEVGSS
Sbjct: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480

Query: 481 FSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540
            SDSI EEICNSAAKMANNLEVDAIFVYT+ GHMASLLSRCRPDCPIFAFT TTSVRRRL
Sbjct: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540

Query: 541 NLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           NLQWGLIPFR+SFS+DMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

[Close]

BLAST of MC07g0650 vs. ExPASy TrEMBL
Match: A0A0A0LKS3 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1)

HSP 1 Score: 1031 bits (2665), Expect = 0.0
Identity = 541/597 (90.62%), Postives = 560/597 (93.80%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQLFT    S  +SL K+S+ K + S+AAFRFPF+ +KSSIRASSS DLNPL +SS
Sbjct: 1   MTQSLQLFT----SSAISLPKHSISKPSSSSAAFRFPFSFSKSSIRASSSSDLNPLSSSS 60

Query: 61  TSQVLVSENGSSGGGG----------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKL 120
           TSQVLVSENGSS GGG          +  V SD++SIEVDAVTEAELKENGFRSTRRTKL
Sbjct: 61  TSQVLVSENGSSSGGGVVFSSAATREFVSVASDSTSIEVDAVTEAELKENGFRSTRRTKL 120

Query: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMD 180
           VCTIGPATCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+ KGYAVAIMMD
Sbjct: 121 VCTIGPATCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDGKGYAVAIMMD 180

Query: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDL 240
           TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDL
Sbjct: 181 TEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIR 360
           DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIES+DSLKNLEEII 
Sbjct: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIIL 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVPSVQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGS 480
           ADVSEAVRQRSDALMLSGESAMGQ+PDKALAVLRSVSLRIE+WWR+EK HEPMELPEVGS
Sbjct: 421 ADVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGS 480

Query: 481 SFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRR 540
           SFSDSI EEICNSAAKMANNLEVDAIFVYT  GHMASLLSRCRPDCPIFAFTSTTSVRRR
Sbjct: 481 SFSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRR 540

Query: 541 LNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           LNLQWGLIPFRLSFS+DMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 591

[Close]

BLAST of MC07g0650 vs. ExPASy TrEMBL
Match: A0A6J1KWK2 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1)

HSP 1 Score: 1030 bits (2664), Expect = 0.0
Identity = 536/598 (89.63%), Postives = 562/598 (93.98%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQ+FT+ TRSP +SL+K+SL K +PSTAAFRFP + TKSSIRASSSPD NPL ++ 
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSSGGGG---------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+GGGG         + PV+SD+++IEVD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDT 180
           CTIGP TCGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLL 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDLL
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG--SDISIIAKIESMDSLKNLEEII 360
           IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG  SDISI+AKIES+DSL NLEEII
Sbjct: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGRCSDISILAKIESLDSLNNLEEII 360

Query: 361 RASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAE 420
            ASDGAMVARGDLGAQIPLEQVP VQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAE
Sbjct: 361 LASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAE 420

Query: 421 VADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVG 480
           VAD+SEAVRQRSDALMLSGESAMGQ+PDKALAVLR+VSLRIE+WWREEK HEPMELPEVG
Sbjct: 421 VADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVG 480

Query: 481 SSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRR 540
           SS SDSI EEICNSAAKMANNLEVDAIFVYT+ GHMASLLSRCRPDCPIFAFT TTSVRR
Sbjct: 481 SSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRR 540

Query: 541 RLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           RLNLQWGLIPFR+SFS+DMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 596

[Close]

BLAST of MC07g0650 vs. ExPASy TrEMBL
Match: A0A5A7UTK2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001510 PE=3 SV=1)

HSP 1 Score: 1027 bits (2656), Expect = 0.0
Identity = 541/596 (90.77%), Postives = 560/596 (93.96%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTKSSIRASSSPDLNPLPASS 60
           M QSLQLFT    S  +SL K+S+ K   STAAFRFPF+ +KSSIRASSS DLNPL +SS
Sbjct: 1   MTQSLQLFT----SSAISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSS-DLNPLSSSS 60

Query: 61  TSQVLVSENGSSGGGG---------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGSS GGG         +  V SD++SI+VDAVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPA+CGFEQLEALAVGGMNVARINMCHGTR+WHR VIERVRRLN+EKGYAVAIMMDT
Sbjct: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLL 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP  ERTINVNYEGFAEDVRVGDDLL
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLP--ERTINVNYEGFAEDVRVGDDLL 240

Query: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300
           VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD
Sbjct: 241 VDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLD 300

Query: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRA 360
           IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIES+DSLKNLEEII A
Sbjct: 301 IDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPSVQQK+VQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGSS 480
           DVSEAVRQRSDALMLSGESAMGQ+PDKALAVLRSVSLRIE+WWR+EK HEPMELPEVGSS
Sbjct: 421 DVSEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSS 480

Query: 481 FSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540
           FSDSI EEICNSAAKMANNLEVDAIFVYT  GHMASLLSRCRPDCPIFAFTSTTSVRRRL
Sbjct: 481 FSDSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRL 540

Query: 541 NLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 587
           NLQWGLIPFRLSFS+DMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

[Close]

BLAST of MC07g0650 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 911.0 bits (2353), Expect = 5.0e-265
Identity = 477/598 (79.77%), Postives = 521/598 (87.12%), Query Frame = 0


Query: 1   MAQSLQLFTTTTRSPTLSLTKNSLPKSAPSTAAFRFPFTLTK-SSIRASS----SPDLNP 60
           M+QS+Q  T +     L L  +   +   S +  RFP T  K +SIRASS    SPDL+ 
Sbjct: 1   MSQSIQFSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLDS 60

Query: 61  LPASSTSQVLVSENGSSGGGG------YAPVVSDASSIEVDAVTEAELKENGFRSTRRTK 120
             +SS+SQVL+S NG+              V +D S IEVD VTEAELKENGFRSTRRTK
Sbjct: 61  SSSSSSSQVLLSPNGTGAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRSTRRTK 120

Query: 121 LVCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLNEEKGYAVAIMM 180
           L+CTIGPATCGFEQLEALAVGGMNVAR+NMCHGTR+WHR VI  VRRLNEEKG+AVAIMM
Sbjct: 121 LICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAVAIMM 180

Query: 181 DTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDD 240
           DTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+   PERTI+V+Y+GFAEDVRVGD+
Sbjct: 181 DTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSR--PERTISVSYDGFAEDVRVGDE 240

Query: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDW 300
           LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDW
Sbjct: 241 LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDW 300

Query: 301 LDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEII 360
           LDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I +IAKIES+DSL NLEEII
Sbjct: 301 LDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEEII 360

Query: 361 RASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAE 420
            ASDGAMVARGDLGAQIPLEQVP+ QQ+IVQ+CR LNKPVIVASQLLESMIEYPTPTRAE
Sbjct: 361 LASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTRAE 420

Query: 421 VADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVG 480
           VADVSEAVRQRSDALMLSGESAMGQFPDKAL VLR+VSLRIERWWREEK HE + L  +G
Sbjct: 421 VADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQAIG 480

Query: 481 SSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRR 540
           SSFSD ISEEICNSAAKMANNL VDA+FVYT  GHMASL+SRCRPDCPIFAFT+TTSVRR
Sbjct: 481 SSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSVRR 540

Query: 541 RLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 588
           RLNLQWGLIPFRLSFS+DME+NLNKTF LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

[Close]

BLAST of MC07g0650 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 367.5 bits (942), Expect = 2.0e-101
Identity = 210/477 (44.03%), Postives = 293/477 (61.43%), Query Frame = 0


Query: 106 RRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTREWHRDVIERVRRLN-EEKGYA 165
           R+TK+VCT+GP+T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 166 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDV 225
           +AIM+DT+G E+  GDL        + G+ +TF++    ST       ++VNY+ F  DV
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST----PSCVSVNYDDFVNDV 229

Query: 226 RVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTI 285
             GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I
Sbjct: 230 EAGDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSI 289

Query: 286 SSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKN 345
           + KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI +I KIES DS+ N
Sbjct: 290 TEKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPN 349

Query: 346 LEEIIRASDGAMVARGDLGAQIPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPT 405
           L  II ASDGAMVARGDLGA++P+E+VP +Q++I+ LCR + K VIVA+ +LESMI +PT
Sbjct: 350 LHSIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPT 409

Query: 406 PTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPME 465
           PTRAEV+D++ AVR+ +DA+MLSGE+A G+FP KA  V+ +V+LR E      +      
Sbjct: 410 PTRAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP---- 469

Query: 466 LPEVGSSFSDSISEEICNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTST 525
            P +G +F + +SE     A  M+N L    + V+TR G MA LLS  RP   I+AFT+ 
Sbjct: 470 -PNLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNE 529

Query: 526 TSVRRRLNLQWGLIPFRLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 582
             +++RL L  G+ P  + F++D E         L  + ++K G+ +  V    Q I
Sbjct: 530 KKIQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

[Close]

BLAST of MC07g0650 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 362.1 bits (928), Expect = 8.6e-100
Identity = 222/521 (42.61%), Postives = 317/521 (60.84%), Query Frame = 0


Query: 67  SENGSSGGGGYAPVVSDASSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCGFEQLEA 126
           +ENG+   G     V D+SS  +     A+ + +   S R+TK+VCTIGP++   E +  
Sbjct: 70  AENGAFDVG-----VLDSSSYRL-----ADSRTSSNDSRRKTKIVCTIGPSSSSREMIWK 129

Query: 127 LAVGGMNVARINMCHGTREWHRDVIERVRRLNE-EKGYAVAIMMDTEGSEIHMGDLGGAS 186
           LA  GMNVAR+NM HG    H+  I+ V+  N      A+AIM+DT+G E+  GD+    
Sbjct: 130 LAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDKAIAIMLDTKGPEVRSGDV--PQ 189

Query: 187 SAKAEDGEIWTFSVRAFDSTLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKI 246
               E+G+ + F+++   S     + T++VNY+ F  DV VGD LLVDGGM+   V  K 
Sbjct: 190 PIFLEEGQEFNFTIKRGVSL----KDTVSVNYDDFVNDVEVGDILLVDGGMMSLAVKSKT 249

Query: 247 GPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAI 306
              VKC+  D G L  R +L        VR ++A LP+I+ KDW DI FG+   VDF A+
Sbjct: 250 SDLVKCVVIDGGELQSRRHLN-------VRGKSATLPSITDKDWEDIKFGVDNQVDFYAV 309

Query: 307 SFVKSAEVIKHLKSYIAARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARGDLGAQ 366
           SFVK A+V+  LK+Y+  ++  +DIS+I KIES DS+KNL  II A DGAMVARGDLGA+
Sbjct: 310 SFVKDAKVVHELKNYL--KTCSADISVIVKIESADSIKNLPSIISACDGAMVARGDLGAE 369

Query: 367 IPLEQVPSVQQKIVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALM 426
           +P+E+VP +Q++I++ CR ++KPVIVA+ +LESMI +PTPTRAEV+D++ AVR+ +DA+M
Sbjct: 370 LPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPTPTRAEVSDIAIAVREGADAIM 429

Query: 427 LSGESAMGQFPDKALAVLRSVSLRIERWWREEKHHEPMELPEVGS-----SFSDSISEEI 486
           LSGE+A G+FP KA+ V+ +V+LR E             LP   S     ++   + +  
Sbjct: 430 LSGETAHGKFPLKAVNVMHTVALRTE-----------ASLPVRTSASRTTAYKGHMGQMF 489

Query: 487 CNSAAKMANNLEVDAIFVYTRKGHMASLLSRCRPDCPIFAFTSTTSVRRRLNLQWGLIPF 546
              A+ MAN L    + V+TR G MA LLS  RP   IFAFT+   + +RL L  G++P 
Sbjct: 490 AFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFTNQRRIMQRLALYQGVMPI 549

Query: 547 RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSI 582
            + FS+D E+   ++  LL+  N++K G  V  V    Q I
Sbjct: 550 YMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPI 553

[Close]

BLAST of MC07g0650 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 245.7 bits (626), Expect = 9.0e-65
Identity = 164/518 (31.66%), Postives = 272/518 (52.51%), Query Frame = 0


Query: 85  SSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTR 144
           S+I+++ + + EL  +G   T +TK+VCT+GPA+     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSH 61

Query: 145 EWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 204
           E+H++ ++ +R   +  G   A+M+DT+G EI  G L   +  + ++G+  T +    D 
Sbjct: 62  EYHQETLDNLRTAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT---DY 121

Query: 205 TLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPR 264
            +   E+TI+++Y+    DV+ G+ +L   G +   V+  +     V C C +  +L  R
Sbjct: 122 DIKGDEKTISMSYKKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGER 181

Query: 265 ANLTFWRDGHLVRERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYI 324
            N+     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  +
Sbjct: 182 KNVNL--PGVVVD-----LPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVL 241

Query: 325 AARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSVQQKIVQL 384
            + S+   I +++K+E+ + + N +EI+R +D  MVARGDLG +IP+E++   Q+ ++  
Sbjct: 242 GSHSK--SIMLMSKVENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYK 301

Query: 385 CRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALA 444
           C    KPV+ A+Q+LESMI+ P PTRAE  DV+ AV   +D +MLSGESA G +P+ A+ 
Sbjct: 302 CNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVK 361

Query: 445 VLRSV------SLRIERWWREEKHHEPMELPEVGSSFSDSISEEICNSAAKMANNLEVDA 504
            +  +      SL     ++E     P+ +         S  E + +SA + AN  +   
Sbjct: 362 TMAKICIEAESSLDYNTIFKEMIRATPLPM---------STLESLASSAVRTANKAKAKL 421

Query: 505 IFVYTRKGHMASLLSRCRPDCPIFA-----FTSTT--------SVRRRLNLQWGLIPF-- 564
           I V TR G  A L+++ RP  PI +     FTS T        S  R   +  GLIP   
Sbjct: 422 IIVLTRGGTTAKLVAKYRPAVPILSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLG 481

Query: 565 ----RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAV 575
               + + SE  E  +         + L   GD V+A+
Sbjct: 482 EGSAKATDSESTEEIIESALKSATEKGLCNHGDAVVAL 495

[Close]

BLAST of MC07g0650 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 237.7 bits (605), Expect = 2.4e-62
Identity = 161/518 (31.08%), Postives = 266/518 (51.35%), Query Frame = 0


Query: 85  SSIEVDAVTEAELKENGFRSTRRTKLVCTIGPATCGFEQLEALAVGGMNVARINMCHGTR 144
           S+I+++ + + EL  +G     +TK+VCT+GPA+     +E L   GMNVAR N  HG+ 
Sbjct: 2   SNIDIEGILK-ELPNDG--RIPKTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSH 61

Query: 145 EWHRDVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDS 204
           E+H++ ++ +R      G   A+M+DT+G EI  G L   +  + ++G+  T +    D 
Sbjct: 62  EYHQETLDNLRSAMHNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT---DY 121

Query: 205 TLPVPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPR 264
            +   E TI+++Y+    DV+ G+ +L   G +   V+  +     V+C C +  +L  R
Sbjct: 122 DIQGDESTISMSYKKLPLDVKPGNTILCADGSISLAVLSCDPESGTVRCRCENSAMLGER 181

Query: 265 ANLTFWRDGHLVRERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYI 324
            N+     G +V      LPT++ KD  DI  +G+   +D +A+SFV+    + +++  +
Sbjct: 182 KNVNL--PGVVVD-----LPTLTDKDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVL 241

Query: 325 AARSRGSDISIIAKIESMDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSVQQKIVQL 384
              S    I +++K+E+ + + N +EI+R +D  MVARGDLG +IP+E++   Q+ ++  
Sbjct: 242 G--SHAKSIMLMSKVENQEGVINFDEILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYK 301

Query: 385 CRQLNKPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALA 444
           C    KPV+ A+Q+LESMI+ P PTRAE  DV+ AV   +D +MLSGESA G +P+ A+ 
Sbjct: 302 CNLAGKPVVTATQMLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVK 361

Query: 445 VLRSV------SLRIERWWREEKHHEPMELPEVGSSFSDSISEEICNSAAKMANNLEVDA 504
           V+  +      SL     ++E     P+ +         S  E + +SA + AN      
Sbjct: 362 VMAKICIEAESSLDYNTIFKEMIRATPLPM---------SPLESLASSAVRTANKARAKL 421

Query: 505 IFVYTRKGHMASLLSRCRPDCPIFAFT-------------STTSVRRRLNLQWGLIPF-- 564
           I V TR G  A+L+++ RP  PI +               S  S  R   +  GLIP   
Sbjct: 422 IIVLTRGGSTANLVAKYRPAVPILSVVVPVMTTDSFDWSCSDESPARHSLIYRGLIPMLA 481

Query: 565 ----RLSFSEDMENNLNKTFLLLKARNLIKSGDLVIAV 575
               + + SE  E  +         R L   GD ++A+
Sbjct: 482 EGSAKATDSEATEVIIEAALKSATQRGLCNRGDAIVAL 495

The following BLAST results are available for this feature:

BLAST of MC07g0650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43117	5.9e-271	82.72	Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1	[more]
Q40545	1.8e-264	80.20	Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9LIK0	7.0e-264	79.77	Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P...	[more]
Q40546	5.8e-101	44.05	Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q9FLW9	2.9e-100	44.03	Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1	[more]

BLAST of MC07g0650 vs. NCBI nr


Match Name	Identity	Description
XP_022138517.1	100.00	pyruvate kinase isozyme A, chloroplastic [Momordica charantia]	[more]
XP_038874381.1	90.60	pyruvate kinase isozyme A, chloroplastic [Benincasa hispida]	[more]
XP_023006710.1	89.93	pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima]	[more]
XP_023548664.1	90.27	pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_004150564.1	90.62	plastidial pyruvate kinase 1, chloroplastic [Cucumis sativus] >KGN61654.1 hypoth...	[more]

BLAST of MC07g0650 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1CBB4	100.00	Pyruvate kinase OS=Momordica charantia OX=3673 GN=LOC111009665 PE=3 SV=1	[more]
A0A6J1L0W9	89.93	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1	[more]
A0A0A0LKS3	90.62	Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_2G215480 PE=3 SV=1	[more]
A0A6J1KWK2	89.63	Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1	[more]
A0A5A7UTK2	90.77	Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G0015...	[more]

BLAST of MC07g0650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22960.1	5.0e-265	79.77	Pyruvate kinase family protein	[more]
AT5G52920.1	2.0e-101	44.03	plastidic pyruvate kinase beta subunit 1	[more]
AT1G32440.1	8.6e-100	42.61	plastidial pyruvate kinase 3	[more]
AT5G63680.1	9.0e-65	31.66	Pyruvate kinase family protein	[more]
AT5G08570.1	2.4e-62	31.08	Pyruvate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001697	Pyruvate kinase	PRINTS	PR01050	PYRUVTKNASE	coord: 300..314 score: 41.74 coord: 163..179 score: 45.07 coord: 359..383 score: 46.83 coord: 409..427 score: 51.34 coord: 428..444 score: 38.46 coord: 384..408 score: 60.31 coord: 332..358 score: 52.19
IPR001697	Pyruvate kinase	TIGRFAM	TIGR01064	TIGR01064	coord: 106..576 e-value: 4.4E-127 score: 422.4
IPR001697	Pyruvate kinase	PANTHER	PTHR11817	PYRUVATE KINASE	coord: 59..587
IPR015806	Pyruvate kinase, insert domain superfamily	GENE3D	2.40.33.10		coord: 174..282 e-value: 1.6E-151 score: 507.0
IPR015795	Pyruvate kinase, C-terminal	PFAM	PF02887	PK_C	coord: 478..577 e-value: 2.2E-22 score: 79.5
IPR036918	Pyruvate kinase, C-terminal domain superfamily	GENE3D	3.40.1380.20		coord: 451..577 e-value: 1.6E-151 score: 507.0
IPR036918	Pyruvate kinase, C-terminal domain superfamily	SUPERFAMILY	52935	PK C-terminal domain-like	coord: 439..585
IPR015793	Pyruvate kinase, barrel	PFAM	PF00224	PK	coord: 106..453 e-value: 1.1E-91 score: 307.4
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 106..450 e-value: 1.6E-151 score: 507.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..73
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..70
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR11817:SF14	PLASTIDIAL PYRUVATE KINASE 1, CHLOROPLASTIC	coord: 59..587
IPR018209	Pyruvate kinase, active site	PROSITE	PS00110	PYRUVATE_KINASE	coord: 330..342
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 92..456
IPR011037	Pyruvate kinase-like, insert domain superfamily	SUPERFAMILY	50800	PK beta-barrel domain-like	coord: 175..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0650.1	MC07g0650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0010431	seed maturation
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030955	potassium ion binding
molecular_function	GO:0004743	pyruvate kinase activity
molecular_function	GO:0003824	catalytic activity