MC07g0595 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0595
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMalate dehydrogenase
LocationMC07: 13966598 .. 13971930 (+)
RNA-Seq ExpressionMC07g0595
SyntenyMC07g0595
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCCGCTGTCGCATCTGAAGCGGAATATGCTTGACGCGCGCATCACGATGATCCCACGCGCGTTCAGACAGAGACCTGTTTTTCCGCCAAGTCAGCACCGTTAAAACCGTGACTTGACGGCCGTCAAGCCCCGGTCGTGGTGACGGCGTCGTTTGATTGGTGACCAGAGAAATTTCTGCTCCCTTAGGTTTCAGTGTCTTCTGTTTCTTCACGCCAATAAATATCCCTTCTTCTTCTTCTTCTTCTTCTTCCTCTACAACCCCCACTGATTCGCGTTCTTCAATCTTCTTCTTCTCCGGCTCTCGTTTCGATTCGTCCTCAGCCATGGCAAAACAACCTGTTAGAGTCCTCGTTACTGGAGCTGCAGGTACTGTACTGAAACCATTGCATTTCTTTCTTTTCGGTTCTACTGATGCTCATCTAGTTGATCTGAGATGTTATCCAATCGCCACTTGATTTCTTTGTCTTTTGCCTCTGCTCGCCTGAGATCTGATACTGTGTTGCTTATCACATCTGTTGTTTGGTTTGAATTTACGATCAGATCTGTTGGTTATAATCCATTTCTTAAAGTTTCTGCTGCGAATTTTCTCACTGGTTGACGTTTTTGCTCGTTCGTTTGCTGTGTTTGAGATTTCGTCTGTTTCTTCATCTTTTTCTGTTAGGAGTTTGAACAATCGCTGAGAGTTTTCGTTCTGTTATGCCAATTCTTTCGTGGATCATTGAGTTTTATTTTTTTACGCGTCCAAGGGTTGCAGTAAGAATGCTATAGGATATAGGCGGAGAAACTGAAGTGGATCTAGCCTGTGAATCTGTGTGGCTTGATGTTTACGAGAGATTTTACTTTGGGTGTTAATGGGCAGATGTCACCAGTTGCATGTTGATTAGTTCTTGTAAACAGAAATCTCGCCTCATGCTGCTCGTATATTTCTTCCTTGTCAAAATTATTTGGATAAGAGTTCCTATTTTTGGTCAATTAAATCCCAACTTATTCTTTTTGAATAGCTATCCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGTGAATAGGTGCACGTGAGCAGTTCTACTGGAATTGATTTGTCATTTCATAGAGGAAATACATTATTAAGACCAAAACTTAATTGATTTGTTTGGCTGACCCCTTAAATATTTTACATATGAAGTAGTATATTTACTGCTTGTTTTTTTCAGGACAAATCGGATATGCCCTTGTGCCTATGATTGCCAGAGGAGTGTTGCTAGGACCTGACCAACCTGTAATCTTGCACATGCTTGATATCCCACCGGCTGCCGAGTCATTGAATGGCGTAAAGATGGAGTTGGTTGATGCTGCTTTCCCACTTCTTAAAGGTATATGATACAGCAATCTTTCACTAATACTGAACGAGCTCCTTCTGTGGTTTCTGTCCTTCGATTTCTGTTGATTATCTTATAATGCATGAATGCCTGGTACAATGTTTTAGGTGTTGTTGCTACAACTGATGTGGTTGAGGCATGCACTGGTGTCAATATTGCTATTATGGTTGGTGGATTCCCAAGAAAAGAGGGTATGGAAAGAAAAGACGTGATGTCTAAAAATGTCTCAATTTACAAGGCTCAGGCTTCTGCCCTTGAAAAGCACGCTGCCGCCAACTGTAAGGTAACTTTTGTTGATTGAATTCCATATAAATAAATAATTTTACTGAAAAGTAATGTTCTTCTAATTTTTTTGAATGGTCAGACAACTGACTCTTGGTCTCCTTGCTCTTGCAGGTCTTGGTTGTTGCTAACCCGGCAAATACTAATGCGTTGATCTTGAAGGAATTTGCTCCCTCCATCCCCGAGAAGAATATTTCTTGCTTGACTAGATTGGACCACAACAGGGCATTAGGCCAAATTTCAGAGAGATTGAATGTTCAAGTATCTGATGTTAAGAATGTTATTATCTGGGGTAACCATTCATCTACACAATACCCTGATGTTAACCATGCTACTGTCAAGTTACCATCTGGTGAGGAGAAACCTGTCCGTGAGCTTGTTGCCGATGATGCATGGTAGAGTTTCTGTACCATATCTGTCCAATTTTAAAGAGACGCATTTGCAATAATATATGGTTTTTTCTTTATTGAAGGTTGAATGGAGAGTTTATTAGCACTGTTCAACAACGTGGTGCTGCAATCATCAAAGCCCGGAAGCTCTCAAGTGCGTTGTCTGCTGCTAGTGCAGCTTGCGATCACATTCGTGATTGGGTTCTTGGAACTCCGGAGGTTGGTGATTTTTCTATAGTCCTTTAACTTTGTGCTTTGAAAGACTGATTTAAAAGCTCATTAAAAACTTGATTTCCTTCCAGGGCACCTGGGTTTCCATGGGAGTTTACTCTGATGGATCTTACAATGTCCCGGCTGGACTGATCTATTCGTTCCCCGTCACCTGTCGTAACGGTGAATGGTCAATTGTGCAAGGTATTGTTTACTTTCCTCCTCAGATACCACGTCATGATCCTGATATACTTTTGATACGTTCATGTTGAGACTCCTTCCAGTAACGTAAAACAATAGATGGTGACAAAAATCTGGTTAATTACAAATTTCGAACCCGAACTTTCAGAATTGTGTCTAATAAGTCTTTATACGACAAGGAGTGTTTGATACGTCTGTCTAACGAGTCCATAGACTTTAAAAAATGTTTAGCTAATTTTTTAGTTTAACCTATAAATTTTGTATACTAAGTTCTTAACTTTCAGTTTTGTGTGATATACGTCCTTGAATTCTGAGTTATACTTATTAAGTTTAGGGATCTATTAGAAAAATATCAAAAAAGTTTTTGGACTTTAAAATTTAAGGACTAAAGATGTAATTTAAGGGTAAAAGTTATGGATTTCTTATGCTGCAAAGTTATAGTCGACGGTGAGATATTTTCTGATTGTGTGTGATCCTCTCTGGATCCAGGACTTTCTGTTGATGAATTCTCAAGGAAGAAACTGGACTTGACTGCAGAGGAGCTCAGCGAGGAAAAGGCTTTGGCTTACTCGTGCCTCTCCTAAACAGCTTTCCCTTCTTCTTCCTCTTCACGTTCGTAGTAAGGTGGCTCTTAATTTACGTACTTTTTGATGAGTGGCTACAGTTTTGAATAATGCCATTGTTAAGAGCTTGAGCTTATATAGGAAGCAGTGGAAACAGGACAATATTTCTGCTGCCTCTGGTTTATGTATTTCCCTCTGTTTTTCCATTTTGTTGAGGATTTTAATTTCCTTACAAGCTTCTTCAACCGGCAATGAAACCCCCATCATCAACTGCCTGTTTGTGTGATGAGTCTTACATGAACTTTGTTGATTGGATTCTCATAATATTTCCCACCTCAATAATTCTCTCCAATTTGTCTTCTTTTTTTTTTTTACAATAACTTATAAGGTAGGATTTAACACATGTCGTTAGGTTGAGCTTGGCTCAGATTCACATTTGTTTGAAATCTTTGCCATGGCTCCCTACAAGGGCATTTTGCCCGGTCATGGCCTTGTTTTCATCTTCGTCCCGGTGGCGGGAATCGAAAGGACTAGTTCTGCCCAGAATCTGGGTTTTCTCAAATTTTCGCTCAATGGGAACCCCAACAACCACGTCTTTGGAATTGAATTTGATGTGTTTGCCAATCAAGAATTCAACGACCCCAACGACAATCATGTGGGAATCAACTTTAATTCTCTGACCTCGAACTCCACCGAAGAGGTTGGGTTTTGGTCGAACGGCGGCCGTAGTGGAGGGGATTCGTTCAATAGACTCAAGCTTAATAGCGGCGAAAATTACCAAGTTTGGATCGACTATTCCGATTTTCTCATGAACGTCACCATGGCGCCGGCGAGGATGAGAACGCCGTGGCGGCCATTGTTTGATGTCTCTGTCAATCTCTCTGAAATTTTCATCGACGGAATATTTGTGGGGTTCACGAGCGCCACCGGGCAGTTGGTACAGAGCCACAAGATTTTGGCCTGGAGTTTCAGTAACATCAATTTTTCGTTAAGCGAGAGTTTGATCACCACTGGTTTGCCCTCATTCGTCCTCCCAAAAGACCCAATCACCAAATCGAAGTGGTTCATCGCCAGAGTGACCGTCAGCATTTCCTCATCCTTTGTTTCCTCGCAGCGATTTCGTGGATTTTGATCGTCGGGCATCGGAGGAGAGCCAGATCCAGAGCGGAAATGGAGGATTGTGAATTGGAGTATTGGCCGCACTGAATCACATATCAAGAAATCGAGTCGGCGACAAAAGAGAAGCCGTCATTGGTGGATTGGGTGCGACGGCTAGCCGAAGAGGTGATTAATATAAAAAAAAACGAAGAGGTGGTCAGACGTTCAAAATTAAAAAAAAATTGCATCTCTTGTCTTCAGTTTTTTAAGACAACAATATCTTTAAAAAAGTTTCAAAACCTACCCTACTTTTAATATTTTTAATTAAACTTCGATATTTTTAAAATGAGTTTCAAAACCTACCATACTTTTAATATTTTTAATTAAACTACGATATTTTTAAAAAAAGTTTCAAAACCTACCATACTTTTAATATTTTTAATTAAACTACGATATTTTTAAAAAAAGTTTCAAAACCTACCATACTTTTAATATTTTTAATTAAACTACGATATTTTTTAAATAAATTTCAAAACCTACCCTACTTTTAATATTTTTAATTAAACTACGATATTTTTAAAATAAATTTCATTTACAATAATATTAATAGAAATAATATTAATAAAAAAAAGCCCATTTTAAACATGTATTTGAAATTAAAATAGCAATTAATTTTTTGAAGTAAATTATTTTACTATTTGGTTGTTTTCTTCCGTACGGAAAAAATATTATAGTGGTATTTTTAAATTTGTCAAATATTACAAAATTTTTCTCCTATTCTATTATGTTTGATAAAGTATACTAGTGATATACTATTTTGATATTATGTATTTATTTTGTATTTTTTGTCATTGTTTTTTTCTTAGTACAACATGTGGGGTGGAGTGAGGGATCGAACTCGCAACTTCATGGTTGGACGTATATGTCAATTACAGCTAACCTGTACTCGCTTTGACTATTTCTGGCCATTATTGAACTAAACAAGAAGCAAAAAGTCTATGCCAATATAAAAATATAGTTTATGTATCCTAAGCTAATTTTATTGTTATTGTAAATGTCTGAAAATATTTGTACTTTAGGCTTTTCATTGTTTTTGCAAATGTCCGAAACTATTTGTATGGTAGGCTTTTCAATATTGATAAAACTATTGTATTGCTGAAGGGAGTCTTAGTGGTAATTGTCATATCATCTCCGATCAAGAAAATTGGTAATGAGAAGAATA

mRNA sequence

TCCCGCTGTCGCATCTGAAGCGGAATATGCTTGACGCGCGCATCACGATGATCCCACGCGCGTTCAGACAGAGACCTGTTTTTCCGCCAAGTCAGCACCGTTAAAACCGTGACTTGACGGCCGTCAAGCCCCGGTCGTGGTGACGGCGTCGTTTGATTGGTGACCAGAGAAATTTCTGCTCCCTTAGGTTTCAGTGTCTTCTGTTTCTTCACGCCAATAAATATCCCTTCTTCTTCTTCTTCTTCTTCTTCCTCTACAACCCCCACTGATTCGCGTTCTTCAATCTTCTTCTTCTCCGGCTCTCGTTTCGATTCGTCCTCAGCCATGGCAAAACAACCTGTTAGAGTCCTCGTTACTGGAGCTGCAGGACAAATCGGATATGCCCTTGTGCCTATGATTGCCAGAGGAGTGTTGCTAGGACCTGACCAACCTGTAATCTTGCACATGCTTGATATCCCACCGGCTGCCGAGTCATTGAATGGCGTAAAGATGGAGTTGGTTGATGCTGCTTTCCCACTTCTTAAAGGTGTTGTTGCTACAACTGATGTGGTTGAGGCATGCACTGGTGTCAATATTGCTATTATGGTTGGTGGATTCCCAAGAAAAGAGGGTATGGAAAGAAAAGACGTGATGTCTAAAAATGTCTCAATTTACAAGGCTCAGGCTTCTGCCCTTGAAAAGCACGCTGCCGCCAACTGTAAGGTCTTGGTTGTTGCTAACCCGGCAAATACTAATGCGTTGATCTTGAAGGAATTTGCTCCCTCCATCCCCGAGAAGAATATTTCTTGCTTGACTAGATTGGACCACAACAGGGCATTAGGCCAAATTTCAGAGAGATTGAATGTTCAAGTATCTGATGTTAAGAATGTTATTATCTGGGGTAACCATTCATCTACACAATACCCTGATGTTAACCATGCTACTGTCAAGTTACCATCTGGTGAGGAGAAACCTGTCCGTGAGCTTGTTGCCGATGATGCATGGTTGAATGGAGAGTTTATTAGCACTGTTCAACAACGTGGTGCTGCAATCATCAAAGCCCGGAAGCTCTCAAGTGCGTTGTCTGCTGCTAGTGCAGCTTGCGATCACATTCGTGATTGGGTTCTTGGAACTCCGGAGGGCACCTGGGTTTCCATGGGAGTTTACTCTGATGGATCTTACAATGTCCCGGCTGGACTGATCTATTCGTTCCCCGTCACCTGTCGTAACGGTGAATGGTCAATTGTGCAAGGACTTTCTGTTGATGAATTCTCAAGGAAGAAACTGGACTTGACTGCAGAGGAGCTCAGCGAGGAAAAGGCTTTGGCTTACTCGTGCCTCTCCTAAACAGCTTTCCCTTCTTCTTCCTCTTCACGTTCGTAGTAAGGTGGCTCTTAATTTACGTACTTTTTGATGAGTGGCTACAGTTTTGAATAATGCCATTGTTAAGAGCTTGAGCTTATATAGGAAGCAGTGGAAACAGGACAATATTTCTGCTGCCTCTGGTTTATGTATTTCCCTCTGTTTTTCCATTTTGTTGAGGATTTTAATTTCCTTACAAGCTTCTTCAACCGGCAATGAAACCCCCATCATCAACTGCCTGTTTGTGTGATGAGTCTTACATGAACTTTGTTGATTGGATTCTCATAATATTTCCCACCTCAATAATTCTCTCCAATTTGTCTTCTTTTTTTTTTTTACAATAACTTATAAGGTAGGATTTAACACATGTCGTTAGGTTGAGCTTGGCTCAGATTCACATTTGTTTGAAATCTTTGCCATGGCTCCCTACAAGGGCATTTTGCCCGGTCATGGCCTTGTTTTCATCTTCGTCCCGGTGGCGGGAATCGAAAGGACTAGTTCTGCCCAGAATCTGGGTTTTCTCAAATTTTCGCTCAATGGGAACCCCAACAACCACGTCTTTGGAATTGAATTTGATGTGTTTGCCAATCAAGAATTCAACGACCCCAACGACAATCATGTGGGAATCAACTTTAATTCTCTGACCTCGAACTCCACCGAAGAGGTTGGGTTTTGGTCGAACGGCGGCCGTAGTGGAGGGGATTCGTTCAATAGACTCAAGCTTAATAGCGGCGAAAATTACCAAGTTTGGATCGACTATTCCGATTTTCTCATGAACGTCACCATGGCGCCGGCGAGGATGAGAACGCCGTGGCGGCCATTGTTTGATGTCTCTGTCAATCTCTCTGAAATTTTCATCGACGGAATATTTGTGGGGTTCACGAGCGCCACCGGGCAGTTGGTACAGAGCCACAAGATTTTGGCCTGGAGTTTCAGTAACATCAATTTTTCGTTAAGCGAGAGTTTGATCACCACTGGTTTGCCCTCATTCGTCCTCCCAAAAGACCCAATCACCAAATCGAAGTGGTTCATCGCCAGAGTGACCGTCAGCATTTCCTCATCCTTTGTTTCCTCGCAGCGATTTCGTGGATTTTGATCGTCGGGCATCGGAGGAGAGCCAGATCCAGAGCGGAAATGGAGGATTGTGAATTGGAGTATTGGCCGCACTGAATCACATATCAAGAAATCGAGTCGGCGACAAAAGAGAAGCCGTCATTGGTGGATTGGGTGCGACGGCTAGCCGAAGAGTACAACATGTGGGGTGGAGTGAGGGATCGAACTCGCAACTTCATGGTTGGACGTATATGTCAATTACAGCTAACCTGTACTCGCTTTGACTATTTCTGGCCATTATTGAACTAAACAAGAAGCAAAAAGTCTATGCCAATATAAAAATATAGTTTATGTATCCTAAGCTAATTTTATTGTTATTGTAAATGTCTGAAAATATTTGTACTTTAGGCTTTTCATTGTTTTTGCAAATGTCCGAAACTATTTGTATGGTAGGCTTTTCAATATTGATAAAACTATTGTATTGCTGAAGGGAGTCTTAGTGGTAATTGTCATATCATCTCCGATCAAGAAAATTGGTAATGAGAAGAATA

Coding sequence (CDS)

ATGGCAAAACAACCTGTTAGAGTCCTCGTTACTGGAGCTGCAGGACAAATCGGATATGCCCTTGTGCCTATGATTGCCAGAGGAGTGTTGCTAGGACCTGACCAACCTGTAATCTTGCACATGCTTGATATCCCACCGGCTGCCGAGTCATTGAATGGCGTAAAGATGGAGTTGGTTGATGCTGCTTTCCCACTTCTTAAAGGTGTTGTTGCTACAACTGATGTGGTTGAGGCATGCACTGGTGTCAATATTGCTATTATGGTTGGTGGATTCCCAAGAAAAGAGGGTATGGAAAGAAAAGACGTGATGTCTAAAAATGTCTCAATTTACAAGGCTCAGGCTTCTGCCCTTGAAAAGCACGCTGCCGCCAACTGTAAGGTCTTGGTTGTTGCTAACCCGGCAAATACTAATGCGTTGATCTTGAAGGAATTTGCTCCCTCCATCCCCGAGAAGAATATTTCTTGCTTGACTAGATTGGACCACAACAGGGCATTAGGCCAAATTTCAGAGAGATTGAATGTTCAAGTATCTGATGTTAAGAATGTTATTATCTGGGGTAACCATTCATCTACACAATACCCTGATGTTAACCATGCTACTGTCAAGTTACCATCTGGTGAGGAGAAACCTGTCCGTGAGCTTGTTGCCGATGATGCATGGTTGAATGGAGAGTTTATTAGCACTGTTCAACAACGTGGTGCTGCAATCATCAAAGCCCGGAAGCTCTCAAGTGCGTTGTCTGCTGCTAGTGCAGCTTGCGATCACATTCGTGATTGGGTTCTTGGAACTCCGGAGGGCACCTGGGTTTCCATGGGAGTTTACTCTGATGGATCTTACAATGTCCCGGCTGGACTGATCTATTCGTTCCCCGTCACCTGTCGTAACGGTGAATGGTCAATTGTGCAAGGACTTTCTGTTGATGAATTCTCAAGGAAGAAACTGGACTTGACTGCAGAGGAGCTCAGCGAGGAAAAGGCTTTGGCTTACTCGTGCCTCTCCTAA

Protein sequence

MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVDAAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKHAAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKARKLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSIVQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS
Homology
BLAST of MC07g0595 vs. ExPASy Swiss-Prot
Match: Q9FSF0 (Malate dehydrogenase OS=Nicotiana tabacum OX=4097 GN=MD1 PE=1 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 3.4e-175
Identity = 311/333 (93.39%), Postives = 323/333 (97.00%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MAK PVRVLVTGAAGQIGYALVPMIARGV+LG DQPVILHMLDIPPAAE+LNGVKMELVD
Sbjct: 1   MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPAAEALNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTD VEACTGVN+A+MVGGFPRKEGMERKDVMSKNVSIYK+QASALEKH
Sbjct: 61  AAFPLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AA NCKVLVVANPANTNALILKE+APSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEYAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSS+QYPDVNHATV  P+G EKPVRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSSQYPDVNHATVATPAG-EKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAAS+ACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPV C+NGEWSI
Sbjct: 241 KLSSALSAASSACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVACKNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           VQGL +DEFSRKKLD TAEELSEEKALAYSCL+
Sbjct: 301 VQGLPIDEFSRKKLDATAEELSEEKALAYSCLT 332

BLAST of MC07g0595 vs. ExPASy Swiss-Prot
Match: O48905 (Malate dehydrogenase, cytoplasmic OS=Medicago sativa OX=3879 GN=CMDH PE=2 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 1.0e-174
Identity = 312/333 (93.69%), Postives = 325/333 (97.60%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MAK PVRVLVTGAAGQIGYALVPMIARGV+LGPDQPVILHMLDI PAAESLNGVKMELVD
Sbjct: 1   MAKDPVRVLVTGAAGQIGYALVPMIARGVMLGPDQPVILHMLDIAPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIA+MVGGFPRKEGMERKDVMSKNVSIYK+QASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPE+NISCLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPERNISCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATV  P+G EKPVR+LV+DDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVNTPAG-EKPVRQLVSDDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTP+GT+VSMGVYSDGSYNVP+GLIYSFPVTC NGEW I
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPQGTFVSMGVYSDGSYNVPSGLIYSFPVTCANGEWKI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           VQGLS+DEFSRKKLDLTAEEL+EEK LA+SCLS
Sbjct: 301 VQGLSIDEFSRKKLDLTAEELTEEKNLAHSCLS 332

BLAST of MC07g0595 vs. ExPASy Swiss-Prot
Match: Q7XDC8 (Malate dehydrogenase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0478200 PE=1 SV=3)

HSP 1 Score: 594.0 bits (1530), Expect = 1.1e-168
Identity = 299/333 (89.79%), Postives = 317/333 (95.20%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MAK+P+RVLVTGAAGQIGYALVPMIARGV+LG DQPVILHMLDIPPA ESLNG+KMELVD
Sbjct: 1   MAKEPMRVLVTGAAGQIGYALVPMIARGVMLGADQPVILHMLDIPPATESLNGLKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKG+VATTDVVEACTGVN+A+MVGGFPRKEGMERKDVMSKNVSIYK+QASALE H
Sbjct: 61  AAFPLLKGIVATTDVVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEAH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AA NCKVLVVANPANTNALILKEFAPSIPEKNI+CLTRLDHNRALGQISE+LNVQV+DVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVTDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           N IIWGNHSSTQYPDVNHATVK PSG EKPVRELVADD WLN EFISTVQQRGAAIIKAR
Sbjct: 181 NAIIWGNHSSTQYPDVNHATVKTPSG-EKPVRELVADDEWLNTEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           K SSALSAAS+ACDHIRDWVLGTPEGT+VSMGVYSDGSY VPAGLIYSFPVTC  GEW+I
Sbjct: 241 KQSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYGVPAGLIYSFPVTCSGGEWTI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           VQGL +DEFSRKK+D TA+ELSEEK LAYSCL+
Sbjct: 301 VQGLPIDEFSRKKMDATAQELSEEKTLAYSCLN 332

BLAST of MC07g0595 vs. ExPASy Swiss-Prot
Match: P93819 (Malate dehydrogenase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=MDH1 PE=1 SV=2)

HSP 1 Score: 593.2 bits (1528), Expect = 1.8e-168
Identity = 299/333 (89.79%), Postives = 319/333 (95.80%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MAK+PVRVLVTGAAGQIGYALVPMIARG++LG DQPVILHMLDIPPAAE+LNGVKMEL+D
Sbjct: 1   MAKEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELID 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTD VE CTGVN+A+MVGGFPRKEGMERKDVMSKNVSIYK+QA+ALEKH
Sbjct: 61  AAFPLLKGVVATTDAVEGCTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AA NCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERL+V VSDVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSS+QYPDVNHA V+  SG EKPVRELV DDAWL+GEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSSQYPDVNHAKVQTSSG-EKPVRELVKDDAWLDGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAAS+ACDHIRDWVLGTPEGT+VSMGVYSDGSY+VP+GLIYSFPVTCRNG+WSI
Sbjct: 241 KLSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYSVPSGLIYSFPVTCRNGDWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           VQGL +DE SRKK+DLTAEEL EEK LAYSCLS
Sbjct: 301 VQGLPIDEVSRKKMDLTAEELKEEKDLAYSCLS 332

BLAST of MC07g0595 vs. ExPASy Swiss-Prot
Match: P57106 (Malate dehydrogenase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=MDH2 PE=1 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 4.1e-168
Identity = 300/333 (90.09%), Postives = 318/333 (95.50%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MAK+PVRVLVTGAAGQIGYALVPMIARG++LG DQPVILHMLDIP AAE+LNGVKMELVD
Sbjct: 1   MAKEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTD VEACTGVN+A+MVGGFPRKEGMERKDVMSKNVSIYK+QASALEKH
Sbjct: 61  AAFPLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AA NCKVLVVANPANTNALILKEFAPSIPEKNI+CLTRLDHNRALGQ+SERL+V VSDVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVK   G EKPVRELV +D WLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKTSVG-EKPVRELVKNDEWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAAS+ACDHIRDWV+GTPEGT+VSMGVYSDGSYNVPAGLIYSFPVTCRNGEW+I
Sbjct: 241 KLSSALSAASSACDHIRDWVVGTPEGTFVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           VQGL +D+ SRKK+DLTAEEL EEK LAYSCLS
Sbjct: 301 VQGLPIDDASRKKMDLTAEELKEEKDLAYSCLS 332

BLAST of MC07g0595 vs. NCBI nr
Match: XP_008460646.1 (PREDICTED: malate dehydrogenase, cytoplasmic [Cucumis melo] >KAA0065647.1 malate dehydrogenase [Cucumis melo var. makuwa] >TYK01434.1 malate dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 641 bits (1653), Expect = 1.96e-231
Identity = 326/333 (97.90%), Postives = 332/333 (99.70%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA++PVRVLVTGAAGQIGYALVPMIARGVLLGP+QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MAQEPVRVLVTGAAGQIGYALVPMIARGVLLGPNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCR+GEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRDGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. NCBI nr
Match: XP_038907087.1 (malate dehydrogenase, cytoplasmic [Benincasa hispida])

HSP 1 Score: 639 bits (1649), Expect = 7.96e-231
Identity = 326/333 (97.90%), Postives = 331/333 (99.40%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA++PVRVLVTGAAGQIGYALVPMIARGVLLGP+QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MAQEPVRVLVTGAAGQIGYALVPMIARGVLLGPNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGV+IAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVSIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADD WLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDEWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLSVDEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSVDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. NCBI nr
Match: XP_023006038.1 (malate dehydrogenase [Cucurbita maxima])

HSP 1 Score: 637 bits (1643), Expect = 6.54e-230
Identity = 324/333 (97.30%), Postives = 331/333 (99.40%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA++PVRVLVTGAAGQIGYALVPMIARGVLLG +QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MAQEPVRVLVTGAAGQIGYALVPMIARGVLLGSNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVS+VK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSEVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGL+YSFPVTCRNGEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLMYSFPVTCRNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. NCBI nr
Match: XP_023548514.1 (malate dehydrogenase [Cucurbita pepo subsp. pepo] >KAG6574915.1 malate dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 637 bits (1642), Expect = 9.29e-230
Identity = 324/333 (97.30%), Postives = 330/333 (99.10%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA +PVRVLVTGAAGQIGYALVPMIARGVLLG +QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MASEPVRVLVTGAAGQIGYALVPMIARGVLLGSNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVS+VK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSEVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGL+YSFPVTCRNGEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLMYSFPVTCRNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. NCBI nr
Match: XP_022959121.1 (malate dehydrogenase [Cucurbita moschata])

HSP 1 Score: 635 bits (1639), Expect = 2.66e-229
Identity = 323/333 (97.00%), Postives = 330/333 (99.10%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA +PVRVLVTGAAGQIGYALVPMIARGVLLG +QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MASEPVRVLVTGAAGQIGYALVPMIARGVLLGSNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVS+VK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSEVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGL+YSFPVTCRNGEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLMYSFPVTCRNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DE+SRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEYSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. ExPASy TrEMBL
Match: A0A5D3BQH8 (Malate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold772G00190 PE=3 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 9.47e-232
Identity = 326/333 (97.90%), Postives = 332/333 (99.70%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA++PVRVLVTGAAGQIGYALVPMIARGVLLGP+QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MAQEPVRVLVTGAAGQIGYALVPMIARGVLLGPNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCR+GEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRDGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. ExPASy TrEMBL
Match: A0A1S3CD07 (Malate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103499421 PE=3 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 9.47e-232
Identity = 326/333 (97.90%), Postives = 332/333 (99.70%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA++PVRVLVTGAAGQIGYALVPMIARGVLLGP+QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MAQEPVRVLVTGAAGQIGYALVPMIARGVLLGPNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCR+GEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRDGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. ExPASy TrEMBL
Match: A0A6J1L3U1 (Malate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111498914 PE=3 SV=1)

HSP 1 Score: 637 bits (1643), Expect = 3.17e-230
Identity = 324/333 (97.30%), Postives = 331/333 (99.40%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA++PVRVLVTGAAGQIGYALVPMIARGVLLG +QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MAQEPVRVLVTGAAGQIGYALVPMIARGVLLGSNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVS+VK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSEVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGL+YSFPVTCRNGEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLMYSFPVTCRNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. ExPASy TrEMBL
Match: A0A6J1KK87 (Malate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111494766 PE=3 SV=1)

HSP 1 Score: 635 bits (1639), Expect = 1.29e-229
Identity = 323/333 (97.00%), Postives = 329/333 (98.80%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA+ PVRVLVTGAAGQIGYALVPMIARGVLLGP+QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MAQNPVRVLVTGAAGQIGYALVPMIARGVLLGPNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNV VS++K
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVHVSEIK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           K SSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTC+NGEWSI
Sbjct: 241 KFSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCQNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLSVDEFSRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSVDEFSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. ExPASy TrEMBL
Match: A0A6J1H3Z1 (Malate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111460212 PE=3 SV=1)

HSP 1 Score: 635 bits (1639), Expect = 1.29e-229
Identity = 323/333 (97.00%), Postives = 330/333 (99.10%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MA +PVRVLVTGAAGQIGYALVPMIARGVLLG +QPVILHMLDIPPAAESLNGVKMELVD
Sbjct: 1   MASEPVRVLVTGAAGQIGYALVPMIARGVLLGSNQPVILHMLDIPPAAESLNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH
Sbjct: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVS+VK
Sbjct: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSEVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVKLPSGEEK VRELVADDAWLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKSVRELVADDAWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGL+YSFPVTCRNGEWSI
Sbjct: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLMYSFPVTCRNGEWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 333
           VQGLS+DE+SRKKLDLTAEEL+EEKALAYSCLS
Sbjct: 301 VQGLSIDEYSRKKLDLTAEELTEEKALAYSCLS 333

BLAST of MC07g0595 vs. TAIR 10
Match: AT1G04410.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-169
Identity = 299/333 (89.79%), Postives = 319/333 (95.80%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MAK+PVRVLVTGAAGQIGYALVPMIARG++LG DQPVILHMLDIPPAAE+LNGVKMEL+D
Sbjct: 1   MAKEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELID 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTD VE CTGVN+A+MVGGFPRKEGMERKDVMSKNVSIYK+QA+ALEKH
Sbjct: 61  AAFPLLKGVVATTDAVEGCTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AA NCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERL+V VSDVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSS+QYPDVNHA V+  SG EKPVRELV DDAWL+GEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSSQYPDVNHAKVQTSSG-EKPVRELVKDDAWLDGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAAS+ACDHIRDWVLGTPEGT+VSMGVYSDGSY+VP+GLIYSFPVTCRNG+WSI
Sbjct: 241 KLSSALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGSYSVPSGLIYSFPVTCRNGDWSI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           VQGL +DE SRKK+DLTAEEL EEK LAYSCLS
Sbjct: 301 VQGLPIDEVSRKKMDLTAEELKEEKDLAYSCLS 332

BLAST of MC07g0595 vs. TAIR 10
Match: AT5G43330.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 592.0 bits (1525), Expect = 2.9e-169
Identity = 300/333 (90.09%), Postives = 318/333 (95.50%), Query Frame = 0

Query: 1   MAKQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVD 60
           MAK+PVRVLVTGAAGQIGYALVPMIARG++LG DQPVILHMLDIP AAE+LNGVKMELVD
Sbjct: 1   MAKEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVD 60

Query: 61  AAFPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKH 120
           AAFPLLKGVVATTD VEACTGVN+A+MVGGFPRKEGMERKDVMSKNVSIYK+QASALEKH
Sbjct: 61  AAFPLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKH 120

Query: 121 AAANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVK 180
           AA NCKVLVVANPANTNALILKEFAPSIPEKNI+CLTRLDHNRALGQ+SERL+V VSDVK
Sbjct: 121 AAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVK 180

Query: 181 NVIIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKAR 240
           NVIIWGNHSSTQYPDVNHATVK   G EKPVRELV +D WLNGEFISTVQQRGAAIIKAR
Sbjct: 181 NVIIWGNHSSTQYPDVNHATVKTSVG-EKPVRELVKNDEWLNGEFISTVQQRGAAIIKAR 240

Query: 241 KLSSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSI 300
           KLSSALSAAS+ACDHIRDWV+GTPEGT+VSMGVYSDGSYNVPAGLIYSFPVTCRNGEW+I
Sbjct: 241 KLSSALSAASSACDHIRDWVVGTPEGTFVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWTI 300

Query: 301 VQGLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           VQGL +D+ SRKK+DLTAEEL EEK LAYSCLS
Sbjct: 301 VQGLPIDDASRKKMDLTAEELKEEKDLAYSCLS 332

BLAST of MC07g0595 vs. TAIR 10
Match: AT5G56720.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 502.3 bits (1292), Expect = 3.0e-142
Identity = 245/331 (74.02%), Postives = 286/331 (86.40%), Query Frame = 0

Query: 3   KQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVDAA 62
           K P+RVL+TGAAG IGYA+ PMIARG++LGPDQP+ILH+LDI PA+ SL  VKMEL D+A
Sbjct: 9   KDPIRVLITGAAGNIGYAIAPMIARGIMLGPDQPMILHLLDIEPASSSLEAVKMELQDSA 68

Query: 63  FPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKHAA 122
           FPLLKGV+ATT+VVEAC  VNI IM+GGFPR  GMERKDVMSKNV IYKAQASALE++A+
Sbjct: 69  FPLLKGVIATTNVVEACKDVNIVIMIGGFPRIAGMERKDVMSKNVVIYKAQASALERYAS 128

Query: 123 ANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNV 182
            +CKVLVVANPANTNALILKEFAPSIPE+NI+CLTRLDHNRAL Q++++L+V VS VKNV
Sbjct: 129 DDCKVLVVANPANTNALILKEFAPSIPEENITCLTRLDHNRALAQLADKLSVPVSSVKNV 188

Query: 183 IIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKARKL 242
           I+WGNHSSTQYPD NHATV   +G ++P++ELV D  WL  EFI  VQQRGAA+++ARK 
Sbjct: 189 IVWGNHSSTQYPDTNHATVSTKTG-DRPLKELVTDHNWLKNEFIVEVQQRGAAVLRARKQ 248

Query: 243 SSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGSYNVPAGLIYSFPVTCRNGEWSIVQ 302
           SSA SAA AACDHIRDW LGTP+GTWVSMGV SDGSY +P GL+YSFPV C  G W IVQ
Sbjct: 249 SSAFSAAGAACDHIRDWFLGTPKGTWVSMGVCSDGSYGIPPGLVYSFPVICEKGSWKIVQ 308

Query: 303 GLSVDEFSRKKLDLTAEELSEEKALAYSCLS 334
           GLS+DEFSR+K+D +A EL+EEK LAYSCL+
Sbjct: 309 GLSIDEFSREKMDDSARELAEEKDLAYSCLN 338

BLAST of MC07g0595 vs. TAIR 10
Match: AT5G58330.2 (lactate/malate dehydrogenase family protein )

HSP 1 Score: 238.4 bits (607), Expect = 8.1e-63
Identity = 137/325 (42.15%), Postives = 194/325 (59.69%), Query Frame = 0

Query: 3   KQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVDAA 62
           K+ + + V+GAAG I   L+  +A G + GPDQP+ L +L    + ++L GV MEL D+ 
Sbjct: 96  KKLINIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSL 155

Query: 63  FPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKHAA 122
           FPLL+ V   TD  E    V  AI++G  PR  GMER D++  N  I+  Q  AL K A+
Sbjct: 156 FPLLREVDIGTDPNEVFQDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAAS 215

Query: 123 ANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNV 182
            N KVLVV NP NTNALI  + AP+IP KN   LTRLD NRA  Q++ +  V    V N+
Sbjct: 216 PNVKVLVVGNPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNM 275

Query: 183 IIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKARKL 242
            IWGNHS+TQ PD  +A +        PV+E++ D  WL   F  +VQ+RG  +I+    
Sbjct: 276 TIWGNHSTTQVPDFLNARI-----NGLPVKEVITDHKWLEEGFTESVQKRGGLLIQKWGR 335

Query: 243 SSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGS-YNVPAGLIYSFPVTCR-NGEWSI 302
           SSA S A +  D I+  V  TPEG W S GVY+DG+ Y +  GL++S P   + +G++ +
Sbjct: 336 SSAASTAVSIVDAIKSLVTPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYEL 395

Query: 303 VQGLSVDEFSRKKLDLTAEELSEEK 326
           V+ + +D++ R+++  +  EL  EK
Sbjct: 396 VKDVEIDDYLRQRIAKSEAELLAEK 415

BLAST of MC07g0595 vs. TAIR 10
Match: AT5G58330.1 (lactate/malate dehydrogenase family protein )

HSP 1 Score: 238.4 bits (607), Expect = 8.1e-63
Identity = 137/325 (42.15%), Postives = 194/325 (59.69%), Query Frame = 0

Query: 3   KQPVRVLVTGAAGQIGYALVPMIARGVLLGPDQPVILHMLDIPPAAESLNGVKMELVDAA 62
           K+ + + V+GAAG I   L+  +A G + GPDQP+ L +L    + ++L GV MEL D+ 
Sbjct: 97  KKLINIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSL 156

Query: 63  FPLLKGVVATTDVVEACTGVNIAIMVGGFPRKEGMERKDVMSKNVSIYKAQASALEKHAA 122
           FPLL+ V   TD  E    V  AI++G  PR  GMER D++  N  I+  Q  AL K A+
Sbjct: 157 FPLLREVDIGTDPNEVFQDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAAS 216

Query: 123 ANCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNV 182
            N KVLVV NP NTNALI  + AP+IP KN   LTRLD NRA  Q++ +  V    V N+
Sbjct: 217 PNVKVLVVGNPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNM 276

Query: 183 IIWGNHSSTQYPDVNHATVKLPSGEEKPVRELVADDAWLNGEFISTVQQRGAAIIKARKL 242
            IWGNHS+TQ PD  +A +        PV+E++ D  WL   F  +VQ+RG  +I+    
Sbjct: 277 TIWGNHSTTQVPDFLNARI-----NGLPVKEVITDHKWLEEGFTESVQKRGGLLIQKWGR 336

Query: 243 SSALSAASAACDHIRDWVLGTPEGTWVSMGVYSDGS-YNVPAGLIYSFPVTCR-NGEWSI 302
           SSA S A +  D I+  V  TPEG W S GVY+DG+ Y +  GL++S P   + +G++ +
Sbjct: 337 SSAASTAVSIVDAIKSLVTPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYEL 396

Query: 303 VQGLSVDEFSRKKLDLTAEELSEEK 326
           V+ + +D++ R+++  +  EL  EK
Sbjct: 397 VKDVEIDDYLRQRIAKSEAELLAEK 416

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FSF03.4e-17593.39Malate dehydrogenase OS=Nicotiana tabacum OX=4097 GN=MD1 PE=1 SV=1[more]
O489051.0e-17493.69Malate dehydrogenase, cytoplasmic OS=Medicago sativa OX=3879 GN=CMDH PE=2 SV=1[more]
Q7XDC81.1e-16889.79Malate dehydrogenase, cytoplasmic OS=Oryza sativa subsp. japonica OX=39947 GN=Os... [more]
P938191.8e-16889.79Malate dehydrogenase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=MDH1 PE=1... [more]
P571064.1e-16890.09Malate dehydrogenase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=MDH2 PE=1... [more]
Match NameE-valueIdentityDescription
XP_008460646.11.96e-23197.90PREDICTED: malate dehydrogenase, cytoplasmic [Cucumis melo] >KAA0065647.1 malate... [more]
XP_038907087.17.96e-23197.90malate dehydrogenase, cytoplasmic [Benincasa hispida][more]
XP_023006038.16.54e-23097.30malate dehydrogenase [Cucurbita maxima][more]
XP_023548514.19.29e-23097.30malate dehydrogenase [Cucurbita pepo subsp. pepo] >KAG6574915.1 malate dehydroge... [more]
XP_022959121.12.66e-22997.00malate dehydrogenase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A5D3BQH89.47e-23297.90Malate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold772... [more]
A0A1S3CD079.47e-23297.90Malate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103499421 PE=3 SV=1[more]
A0A6J1L3U13.17e-23097.30Malate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111498914 PE=3 SV=1[more]
A0A6J1KK871.29e-22997.00Malate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111494766 PE=3 SV=1[more]
A0A6J1H3Z11.29e-22997.00Malate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111460212 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G04410.11.3e-16989.79Lactate/malate dehydrogenase family protein [more]
AT5G43330.12.9e-16990.09Lactate/malate dehydrogenase family protein [more]
AT5G56720.13.0e-14274.02Lactate/malate dehydrogenase family protein [more]
AT5G58330.28.1e-6342.15lactate/malate dehydrogenase family protein [more]
AT5G58330.18.1e-6342.15lactate/malate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010945Malate dehydrogenase, type 2TIGRFAMTIGR01759TIGR01759coord: 3..327
e-value: 4.3E-155
score: 513.6
IPR010945Malate dehydrogenase, type 2PANTHERPTHR23382MALATE DEHYDROGENASEcoord: 1..333
IPR011274Malate dehydrogenase, NAD-dependent, cytosolicTIGRFAMTIGR01758TIGR01758coord: 7..330
e-value: 3.1E-177
score: 586.8
IPR011274Malate dehydrogenase, NAD-dependent, cytosolicCDDcd01336MDH_cytoplasmic_cytosoliccoord: 4..329
e-value: 0.0
score: 620.797
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalGENE3D3.90.110.10coord: 156..333
e-value: 8.0E-77
score: 258.9
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalSUPERFAMILY56327LDH C-terminal domain-likecoord: 157..332
IPR001557L-lactate/malate dehydrogenasePIRSFPIRSF000102Lac_mal_DHcoord: 2..331
e-value: 1.9E-65
score: 219.0
NoneNo IPR availableGENE3D3.40.50.720coord: 3..155
e-value: 5.4E-62
score: 210.2
NoneNo IPR availablePIRSRPIRSR000102-1PIRSR000102-1coord: 4..326
e-value: 4.0E-69
score: 231.0
NoneNo IPR availablePIRSRPIRSR000102-2PIRSR000102-2coord: 4..326
e-value: 4.0E-69
score: 231.0
NoneNo IPR availablePANTHERPTHR23382:SF26MALATE DEHYDROGENASEcoord: 1..333
IPR001236Lactate/malate dehydrogenase, N-terminalPFAMPF00056Ldh_1_Ncoord: 6..153
e-value: 5.5E-33
score: 114.0
IPR022383Lactate/malate dehydrogenase, C-terminalPFAMPF02866Ldh_1_Ccoord: 157..326
e-value: 2.2E-36
score: 125.4
IPR001252Malate dehydrogenase, active sitePROSITEPS00068MDHcoord: 156..168
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 4..154

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0595.1MC07g0595.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006108 malate metabolic process
biological_process GO:0006734 NADH metabolic process
biological_process GO:0006107 oxaloacetate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
biological_process GO:0019752 carboxylic acid metabolic process
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0030060 L-malate dehydrogenase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016615 malate dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor