MC07g0572 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0572
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming)
Location	MC07: 13738374 .. 13738565 (+)
RNA-Seq Expression	MC07g0572
Synteny	MC07g0572

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTGGCGGTGATCGGAGCGGGGATCAGCGGCCTGGTTTCGGCTTATGTTTTGGCCAAAGCCGGGGCGGAGGTCGTGTTGTTTGAAAGGGAAGATCATTTAGGTGGTCATGCCACAACTCTTAACTCCCATGGATTTGATTTGGACCTTGGCTTCATGCTCTCCAATTCAGTAATCTCTCTCGTTCTT

mRNA sequence

ATGAAGGTGGCGGTGATCGGAGCGGGGATCAGCGGCCTGGTTTCGGCTTATGTTTTGGCCAAAGCCGGGGCGGAGGTCGTGTTGTTTGAAAGGGAAGATCATTTAGGTGGTCATGCCACAACTCTTAACTCCCATGGATTTGATTTGGACCTTGGCTTCATGCTCTCCAATTCAGTAATCTCTCTCGTTCTT

Coding sequence (CDS)

ATGAAGGTGGCGGTGATCGGAGCGGGGATCAGCGGCCTGGTTTCGGCTTATGTTTTGGCCAAAGCCGGGGCGGAGGTCGTGTTGTTTGAAAGGGAAGATCATTTAGGTGGTCATGCCACAACTCTTAACTCCCATGGATTTGATTTGGACCTTGGCTTCATGCTCTCCAATTCAGTAATCTCTCTCGTTCTT

Protein sequence

MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSVISLVL

Homology

[Close]

BLAST of MC07g0572 vs. ExPASy Swiss-Prot
Match: Q2FDU6 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain USA300) OX=367830 GN=crtN PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.9e-06
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          MK+AVIGAG++GL +A  +A  G EV +FE+ +++GG    L   GF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of MC07g0572 vs. ExPASy Swiss-Prot
Match: Q2FV60 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain NCTC 8325 / PS 47) OX=93061 GN=crtN PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.9e-06
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          MK+AVIGAG++GL +A  +A  G EV +FE+ +++GG    L   GF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of MC07g0572 vs. ExPASy Swiss-Prot
Match: O07855 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain Newman) OX=426430 GN=crtN PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 2.9e-06
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          MK+AVIGAG++GL +A  +A  G EV +FE+ +++GG    L   GF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of MC07g0572 vs. ExPASy Swiss-Prot
Match: Q99R76 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=crtN PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.9e-06
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          MK+AVIGAG++GL +A  +A  G EV +FE+ +++GG    L   GF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of MC07g0572 vs. ExPASy Swiss-Prot
Match: Q7A3E2 (4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus aureus (strain N315) OX=158879 GN=crtN PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 2.9e-06
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          MK+AVIGAG++GL +A  +A  G EV +FE+ +++GG    L   GF  D+G
Sbjct: 1  MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNNNVGGRMNQLKKDGFTFDMG 52

[Close]

BLAST of MC07g0572 vs. NCBI nr
Match: KDO50391.1 (hypothetical protein CISIN_1g0358702mg, partial [Citrus sinensis])

HSP 1 Score: 84.7 bits (208), Expect = 5.76e-20
Identity = 43/57 (75.44%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSN 57
          M+VAVIGAGISGLVSAYVLAKAG EVVL+E++D+LGGHA T+   G DLDLGFM+ N
Sbjct: 1  MRVAVIGAGISGLVSAYVLAKAGVEVVLYEKDDYLGGHAKTVTFDGVDLDLGFMVFN 57

[Close]

BLAST of MC07g0572 vs. NCBI nr
Match: KAE9612106.1 (putative protoporphyrinogen oxidase [Lupinus albus])

HSP 1 Score: 85.1 bits (209), Expect = 1.38e-19
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSVI 60
          M+VAV+GAGISGLVSAYVLAK G  VVL+E+ED+LGGHA T+N  G DLDLGFM+ N + 
Sbjct: 1  MRVAVVGAGISGLVSAYVLAKGGVNVVLYEKEDYLGGHAKTVNVDGVDLDLGFMVFNRMS 60

Query: 61 SLV 63
           LV
Sbjct: 61 ILV 63

[Close]

BLAST of MC07g0572 vs. NCBI nr
Match: PNX88991.1 (cyclopropane-fatty-acyl-phospholipid synthase, partial [Trifolium pratense])

HSP 1 Score: 83.6 bits (205), Expect = 1.65e-19
Identity = 41/57 (71.93%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSN 57
          M+VAV+G GISGLVSAYVL KAG  VVL+E+ED+LGGHA T+N+ G DLDLGFM+ N
Sbjct: 1  MRVAVVGGGISGLVSAYVLVKAGINVVLYEKEDYLGGHAKTVNADGVDLDLGFMVFN 57

[Close]

BLAST of MC07g0572 vs. NCBI nr
Match: XP_038875017.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC120067539 [Benincasa hispida])

HSP 1 Score: 90.9 bits (224), Expect = 2.38e-19
Identity = 46/59 (77.97%), Postives = 52/59 (88.14%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSV 59
          MKVAV+GAG SGLVSAYVLAKAG EVVLFE+ED+LGGH+ T+N  GFDLDLGFM+ N V
Sbjct: 1  MKVAVVGAGXSGLVSAYVLAKAGVEVVLFEKEDYLGGHSKTVNFDGFDLDLGFMVFNRV 59

[Close]

BLAST of MC07g0572 vs. NCBI nr
Match: TYJ95684.1 (cyclopropane-fatty-acyl-phospholipid synthase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 83.2 bits (204), Expect = 2.53e-19
Identity = 41/59 (69.49%), Postives = 52/59 (88.14%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSV 59
          MKVAV+G GISGLVSA+VLA+AG EVVLFE+ED++GGH+ T++  G DLDLGFM+ N+V
Sbjct: 3  MKVAVVGGGISGLVSAFVLAEAGVEVVLFEKEDYVGGHSKTVHFDGVDLDLGFMVFNTV 61

[Close]

BLAST of MC07g0572 vs. ExPASy TrEMBL
Match: A0A067EHI4 (Uncharacterized protein (Fragment) OS=Citrus sinensis OX=2711 GN=CISIN_1g0358702mg PE=4 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.79e-20
Identity = 43/57 (75.44%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSN 57
          M+VAVIGAGISGLVSAYVLAKAG EVVL+E++D+LGGHA T+   G DLDLGFM+ N
Sbjct: 1  MRVAVIGAGISGLVSAYVLAKAGVEVVLYEKDDYLGGHAKTVTFDGVDLDLGFMVFN 57

[Close]

BLAST of MC07g0572 vs. ExPASy TrEMBL
Match: A0A6A4QF48 (Putative protoporphyrinogen oxidase OS=Lupinus albus OX=3870 GN=Lalb_Chr06g0169441 PE=4 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 6.69e-20
Identity = 44/63 (69.84%), Postives = 52/63 (82.54%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSVI 60
          M+VAV+GAGISGLVSAYVLAK G  VVL+E+ED+LGGHA T+N  G DLDLGFM+ N + 
Sbjct: 1  MRVAVVGAGISGLVSAYVLAKGGVNVVLYEKEDYLGGHAKTVNVDGVDLDLGFMVFNRMS 60

Query: 61 SLV 63
           LV
Sbjct: 61 ILV 63

[Close]

BLAST of MC07g0572 vs. ExPASy TrEMBL
Match: A0A2K3MDX5 (Cyclopropane-fatty-acyl-phospholipid synthase (Fragment) OS=Trifolium pratense OX=57577 GN=L195_g045106 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 8.00e-20
Identity = 41/57 (71.93%), Postives = 49/57 (85.96%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSN 57
          M+VAV+G GISGLVSAYVL KAG  VVL+E+ED+LGGHA T+N+ G DLDLGFM+ N
Sbjct: 1  MRVAVVGGGISGLVSAYVLVKAGINVVLYEKEDYLGGHAKTVNADGVDLDLGFMVFN 57

[Close]

BLAST of MC07g0572 vs. ExPASy TrEMBL
Match: A0A5K0Z1P0 (Uncharacterized protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS9251 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 8.00e-20
Identity = 40/57 (70.18%), Postives = 50/57 (87.72%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSN 57
          M+VAVIG G+SGLVSAY+LAKAG  VVL+E+ DHLGGHA T++++G DLDLGFM+ N
Sbjct: 1  MRVAVIGGGVSGLVSAYILAKAGVHVVLYEKADHLGGHAHTVHANGVDLDLGFMVFN 57

[Close]

BLAST of MC07g0572 vs. ExPASy TrEMBL
Match: A0A5D3B9S3 (Cyclopropane-fatty-acyl-phospholipid synthase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold726G00100 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.23e-19
Identity = 41/59 (69.49%), Postives = 52/59 (88.14%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSV 59
          MKVAV+G GISGLVSA+VLA+AG EVVLFE+ED++GGH+ T++  G DLDLGFM+ N+V
Sbjct: 3  MKVAVVGGGISGLVSAFVLAEAGVEVVLFEKEDYVGGHSKTVHFDGVDLDLGFMVFNTV 61

[Close]

BLAST of MC07g0572 vs. TAIR 10
Match: AT3G23530.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 73.9 bits (180), Expect = 5.1e-14
Identity = 41/60 (68.33%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGA-EVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSV 60
          MKVAVIG+GISGL SAYVLA  G  EVVL+E+E+ LGGHA T+   G DLDLGFM+ N V
Sbjct: 1  MKVAVIGSGISGLGSAYVLANQGVKEVVLYEKEESLGGHAKTVRFDGVDLDLGFMVFNRV 60

[Close]

BLAST of MC07g0572 vs. TAIR 10
Match: AT3G23510.1 (Cyclopropane-fatty-acyl-phospholipid synthase )

HSP 1 Score: 73.9 bits (180), Expect = 5.1e-14
Identity = 41/60 (68.33%), Postives = 47/60 (78.33%), Query Frame = 0


Query: 1  MKVAVIGAGISGLVSAYVLAKAGA-EVVLFEREDHLGGHATTLNSHGFDLDLGFMLSNSV 60
          MKVAVIG+GISGL SAYVLA  G  EVVL+E+E+ LGGHA T+   G DLDLGFM+ N V
Sbjct: 1  MKVAVIGSGISGLGSAYVLANQGVKEVVLYEKEESLGGHAKTVRFDGVDLDLGFMVFNRV 60

[Close]

BLAST of MC07g0572 vs. TAIR 10
Match: AT5G14220.1 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 42.4 bits (98), Expect = 1.6e-04
Identity = 21/51 (41.18%), Postives = 31/51 (60.78%), Query Frame = 0


Query: 2  KVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          +VAV+GAG+SGL +AY L   G  V +FE +  +GG   ++  +G   D G
Sbjct: 18 RVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGRVGGKLRSVMQNGLIWDEG 68

[Close]

BLAST of MC07g0572 vs. TAIR 10
Match: AT5G14220.2 (Flavin containing amine oxidoreductase family )

HSP 1 Score: 42.4 bits (98), Expect = 1.6e-04
Identity = 21/51 (41.18%), Postives = 31/51 (60.78%), Query Frame = 0


Query: 2  KVAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          +VAV+GAG+SGL +AY L   G  V +FE +  +GG   ++  +G   D G
Sbjct: 18 RVAVVGAGVSGLAAAYKLKSRGLNVTVFEADGRVGGKLRSVMQNGLIWDEG 68

[Close]

BLAST of MC07g0572 vs. TAIR 10
Match: AT3G59050.1 (polyamine oxidase 3 )

HSP 1 Score: 41.2 bits (95), Expect = 3.7e-04
Identity = 22/50 (44.00%), Postives = 30/50 (60.00%), Query Frame = 0


Query: 3  VAVIGAGISGLVSAYVLAKAGAEVVLFEREDHLGGHATTLNSHGFDLDLG 53
          V VIG G++G+ +A  L  A  +VV+ E  D +GG   T  S GF +DLG
Sbjct: 31 VIVIGGGMAGISAARTLQDASFQVVVLESRDRIGGRVHTDYSFGFPVDLG 80

The following BLAST results are available for this feature:

BLAST of MC07g0572 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2FDU6	2.9e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]
Q2FV60	2.9e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]
O07855	2.9e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]
Q99R76	2.9e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]
Q7A3E2	2.9e-06	46.15	4,4'-diapophytoene desaturase (4,4'-diaponeurosporene-forming) OS=Staphylococcus...	[more]

BLAST of MC07g0572 vs. NCBI nr


Match Name	E-value	Identity	Description
KDO50391.1	5.76e-20	75.44	hypothetical protein CISIN_1g0358702mg, partial [Citrus sinensis]	[more]
KAE9612106.1	1.38e-19	69.84	putative protoporphyrinogen oxidase [Lupinus albus]	[more]
PNX88991.1	1.65e-19	71.93	cyclopropane-fatty-acyl-phospholipid synthase, partial [Trifolium pratense]	[more]
XP_038875017.1	2.38e-19	77.97	LOW QUALITY PROTEIN: uncharacterized protein LOC120067539 [Benincasa hispida]	[more]
TYJ95684.1	2.53e-19	69.49	cyclopropane-fatty-acyl-phospholipid synthase family protein [Cucumis melo var. ...	[more]

BLAST of MC07g0572 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A067EHI4	2.79e-20	75.44	Uncharacterized protein (Fragment) OS=Citrus sinensis OX=2711 GN=CISIN_1g0358702...	[more]
A0A6A4QF48	6.69e-20	69.84	Putative protoporphyrinogen oxidase OS=Lupinus albus OX=3870 GN=Lalb_Chr06g01694...	[more]
A0A2K3MDX5	8.00e-20	71.93	Cyclopropane-fatty-acyl-phospholipid synthase (Fragment) OS=Trifolium pratense O...	[more]
A0A5K0Z1P0	8.00e-20	70.18	Uncharacterized protein (Fragment) OS=Nymphaea colorata OX=210225 GN=NYM_LOCUS92...	[more]
A0A5D3B9S3	1.23e-19	69.49	Cyclopropane-fatty-acyl-phospholipid synthase family protein OS=Cucumis melo var...	[more]

BLAST of MC07g0572 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23530.1	5.1e-14	68.33	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT3G23510.1	5.1e-14	68.33	Cyclopropane-fatty-acyl-phospholipid synthase	[more]
AT5G14220.1	1.6e-04	41.18	Flavin containing amine oxidoreductase family	[more]
AT5G14220.2	1.6e-04	41.18	Flavin containing amine oxidoreductase family	[more]
AT3G59050.1	3.7e-04	44.00	polyamine oxidase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00419	ADXRDTASE	coord: 25..38 score: 33.67 coord: 2..24 score: 45.03
None	No IPR available	PFAM	PF13450	NAD_binding_8	coord: 5..54 e-value: 5.4E-16 score: 58.6
None	No IPR available	PANTHER	PTHR42923	PROTOPORPHYRINOGEN OXIDASE	coord: 1..58
None	No IPR available	PANTHER	PTHR42923:SF17	OS12G0267100 PROTEIN	coord: 1..58
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 1..61 e-value: 9.6E-17 score: 63.5
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 1..55

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0572.1	MC07g0572.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0016491	oxidoreductase activity