MC07g0528 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0528
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	9-cis-epoxycarotenoid dioxygenase, chloroplastic
Location	MC07: 13350204 .. 13351913 (-)
RNA-Seq Expression	MC07g0528
Synteny	MC07g0528

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAATCTGTTTACAGTCGCCGCCGCCGCCGCCACTTTTCCGGAGCTCTTCCTCCAAAGTGCTGGTGGACCCCACCATTGGAACCCTCCCGATAAGAAAACTCGAATTATCGCCACCTCCGCCCGAAACGGTGTCGTCTTTTCCCTTACCGATTCTACCACGTCAGCTGAACCCGTTTCAGAAATTGGCGGAATCGCTGCTGGACGCCGTGGAGGCGTTGCTGGTAGTTGAAAAACTGCCAAAAACGGTCGACCCGGCGGTTCATATTTCCGGCAACTTCTCCCCGGTTCCCGAATTCCCGGTTCGCCACGACCTCGAAGTCGTCGGAGAATTTCCTGCATGCTTACGTGGCGTCTACTTGAGAAACGGCGCTAATCCCATGCATGCACCCTTAAGTGGGCACCACTTGTTTGACGGTGACGGAATGGTCCATGCCGTTAAATTAGAAGCCGGGAACAAAGCCAGCTACAGCTGCCGGTTCACTCGCACGAGCCGGCTCGAGCAGGAGGCGGCTTTGGGGCGAGCCGTATTCCCCAAGCCGATCGGCGAGCTGCATGGTCACCAGGGGCTGGCACGGCTCGGGGTTTTTCTCGCTCGATCCGGGATCGGGTTAATCGACGGCTCTAAAGGGACCGGCGTGGCCAACACCGGTTTGGTTTATTTCAACGGTAGATTATTGGCTTTGTCCGAAGACGATCTTCCGTATCACGTAAAAATCATGGACGATGGAGATCTGGAAACGATCGGACGGTTCAGCTTCAACGATCAGCTTAACTGTCCAATGATTGCTCACCCGAAAGTGGACCCCATTTTGGGCGATCTCCACGGTCTGAGCTACAACATGGTAAAAAAACCATATTTGAAATATTTACGGTTTGACCGCGACGGTAAAAAATCGCGTGACGTGGCCATCACGCTGCGGGAGGCGACTATGATTCACGACTTCGCGATAACGGAGAACCACGTGGTGATACCGGACCATCAAGTGGTTTTTAAATTGTCCGAAATGGTTCGGGGCGGGTCGCCCGTTGTTTTCGACCCGAATAAAACTTCCCGGTTCGGAATCTTGCCGAAATCCGACGTGGATGAGGAGGAGATCGTTTGGATCGACGTTCCGGATTGCTTCTGCTTCCATTTGTGGAATGCGTGGGAGGAGACCGCTGGCGACGGTGAGACAACCGTCGTCGTTATCGGGTCTTGTATGAACCCGCCCGATTCGATTTTCAACGACCGGGACCGGGCGTTGACGATTGAGCTCACGGAGATTCGGATGAACATTAGGACCCGGGAGGTGACCCGGCGGGTGTTCGGGTCTGGTTTGAATTTGGAAGCGGGTCAGGTAAACCGGCGGTTTATTGGGCGGAGAACCCGTTTCGTGTATTTGGCTATAGCGGAGCCGTGGCCGAAGTGCTCCGGCATTGCGAAGGTGGATCTGAAAACCGGGAAGGTGGAGAAGTTTTTGTACGGCGACGGCCGGTACGGCGGCGAACCGTTTTTTGTGGCGGAAAATGGGAGCGGCGGCGGAGATCATGAGGACGGAGGGTTTGTGGTGGGGTTTGTGAGGGATGAGAAGAGGGAGACGTCGGAGCTGGTGGTGGTGGAGGCGGCGGAGATGAAGGAAGTGGCGGCAGTGAGGCTGCCGGCGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGATTTGAAGGGGCAAGCTCCG

mRNA sequence

ATGCAAATCTGTTTACAGTCGCCGCCGCCGCCGCCACTTTTCCGGAGCTCTTCCTCCAAAGTGCTGGTGGACCCCACCATTGGAACCCTCCCGATAAGAAAACTCGAATTATCGCCACCTCCGCCCGAAACGGTGTCGTCTTTTCCCTTACCGATTCTACCACGTCAGCTGAACCCGTTTCAGAAATTGGCGGAATCGCTGCTGGACGCCGTGGAGGCGTTGCTGGTAGTTGAAAAACTGCCAAAAACGGTCGACCCGGCGGTTCATATTTCCGGCAACTTCTCCCCGGTTCCCGAATTCCCGGTTCGCCACGACCTCGAAGTCGTCGGAGAATTTCCTGCATGCTTACGTGGCGTCTACTTGAGAAACGGCGCTAATCCCATGCATGCACCCTTAAGTGGGCACCACTTGTTTGACGGTGACGGAATGGTCCATGCCGTTAAATTAGAAGCCGGGAACAAAGCCAGCTACAGCTGCCGGTTCACTCGCACGAGCCGGCTCGAGCAGGAGGCGGCTTTGGGGCGAGCCGTATTCCCCAAGCCGATCGGCGAGCTGCATGGTCACCAGGGGCTGGCACGGCTCGGGGTTTTTCTCGCTCGATCCGGGATCGGGTTAATCGACGGCTCTAAAGGGACCGGCGTGGCCAACACCGGTTTGGTTTATTTCAACGGTAGATTATTGGCTTTGTCCGAAGACGATCTTCCGTATCACGTAAAAATCATGGACGATGGAGATCTGGAAACGATCGGACGGTTCAGCTTCAACGATCAGCTTAACTGTCCAATGATTGCTCACCCGAAAGTGGACCCCATTTTGGGCGATCTCCACGGTCTGAGCTACAACATGGTAAAAAAACCATATTTGAAATATTTACGGTTTGACCGCGACGGTAAAAAATCGCGTGACGTGGCCATCACGCTGCGGGAGGCGACTATGATTCACGACTTCGCGATAACGGAGAACCACGTGGTGATACCGGACCATCAAGTGGTTTTTAAATTGTCCGAAATGGTTCGGGGCGGGTCGCCCGTTGTTTTCGACCCGAATAAAACTTCCCGGTTCGGAATCTTGCCGAAATCCGACGTGGATGAGGAGGAGATCGTTTGGATCGACGTTCCGGATTGCTTCTGCTTCCATTTGTGGAATGCGTGGGAGGAGACCGCTGGCGACGGTGAGACAACCGTCGTCGTTATCGGGTCTTGTATGAACCCGCCCGATTCGATTTTCAACGACCGGGACCGGGCGTTGACGATTGAGCTCACGGAGATTCGGATGAACATTAGGACCCGGGAGGTGACCCGGCGGGTGTTCGGGTCTGGTTTGAATTTGGAAGCGGGTCAGGTAAACCGGCGGTTTATTGGGCGGAGAACCCGTTTCGTGTATTTGGCTATAGCGGAGCCGTGGCCGAAGTGCTCCGGCATTGCGAAGGTGGATCTGAAAACCGGGAAGGTGGAGAAGTTTTTGTACGGCGACGGCCGGTACGGCGGCGAACCGTTTTTTGTGGCGGAAAATGGGAGCGGCGGCGGAGATCATGAGGACGGAGGGTTTGTGGTGGGGTTTGTGAGGGATGAGAAGAGGGAGACGTCGGAGCTGGTGGTGGTGGAGGCGGCGGAGATGAAGGAAGTGGCGGCAGTGAGGCTGCCGGCGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGATTTGAAGGGGCAAGCTCCG

Coding sequence (CDS)

ATGCAAATCTGTTTACAGTCGCCGCCGCCGCCGCCACTTTTCCGGAGCTCTTCCTCCAAAGTGCTGGTGGACCCCACCATTGGAACCCTCCCGATAAGAAAACTCGAATTATCGCCACCTCCGCCCGAAACGGTGTCGTCTTTTCCCTTACCGATTCTACCACGTCAGCTGAACCCGTTTCAGAAATTGGCGGAATCGCTGCTGGACGCCGTGGAGGCGTTGCTGGTAGTTGAAAAACTGCCAAAAACGGTCGACCCGGCGGTTCATATTTCCGGCAACTTCTCCCCGGTTCCCGAATTCCCGGTTCGCCACGACCTCGAAGTCGTCGGAGAATTTCCTGCATGCTTACGTGGCGTCTACTTGAGAAACGGCGCTAATCCCATGCATGCACCCTTAAGTGGGCACCACTTGTTTGACGGTGACGGAATGGTCCATGCCGTTAAATTAGAAGCCGGGAACAAAGCCAGCTACAGCTGCCGGTTCACTCGCACGAGCCGGCTCGAGCAGGAGGCGGCTTTGGGGCGAGCCGTATTCCCCAAGCCGATCGGCGAGCTGCATGGTCACCAGGGGCTGGCACGGCTCGGGGTTTTTCTCGCTCGATCCGGGATCGGGTTAATCGACGGCTCTAAAGGGACCGGCGTGGCCAACACCGGTTTGGTTTATTTCAACGGTAGATTATTGGCTTTGTCCGAAGACGATCTTCCGTATCACGTAAAAATCATGGACGATGGAGATCTGGAAACGATCGGACGGTTCAGCTTCAACGATCAGCTTAACTGTCCAATGATTGCTCACCCGAAAGTGGACCCCATTTTGGGCGATCTCCACGGTCTGAGCTACAACATGGTAAAAAAACCATATTTGAAATATTTACGGTTTGACCGCGACGGTAAAAAATCGCGTGACGTGGCCATCACGCTGCGGGAGGCGACTATGATTCACGACTTCGCGATAACGGAGAACCACGTGGTGATACCGGACCATCAAGTGGTTTTTAAATTGTCCGAAATGGTTCGGGGCGGGTCGCCCGTTGTTTTCGACCCGAATAAAACTTCCCGGTTCGGAATCTTGCCGAAATCCGACGTGGATGAGGAGGAGATCGTTTGGATCGACGTTCCGGATTGCTTCTGCTTCCATTTGTGGAATGCGTGGGAGGAGACCGCTGGCGACGGTGAGACAACCGTCGTCGTTATCGGGTCTTGTATGAACCCGCCCGATTCGATTTTCAACGACCGGGACCGGGCGTTGACGATTGAGCTCACGGAGATTCGGATGAACATTAGGACCCGGGAGGTGACCCGGCGGGTGTTCGGGTCTGGTTTGAATTTGGAAGCGGGTCAGGTAAACCGGCGGTTTATTGGGCGGAGAACCCGTTTCGTGTATTTGGCTATAGCGGAGCCGTGGCCGAAGTGCTCCGGCATTGCGAAGGTGGATCTGAAAACCGGGAAGGTGGAGAAGTTTTTGTACGGCGACGGCCGGTACGGCGGCGAACCGTTTTTTGTGGCGGAAAATGGGAGCGGCGGCGGAGATCATGAGGACGGAGGGTTTGTGGTGGGGTTTGTGAGGGATGAGAAGAGGGAGACGTCGGAGCTGGTGGTGGTGGAGGCGGCGGAGATGAAGGAAGTGGCGGCAGTGAGGCTGCCGGCGAGGGTTCCGTACGGATTTCACGGCACATTTGTTAGTGAAGAAGATTTGAAGGGGCAAGCTCCG

Protein sequence

MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLELSPPPPETVSSFPLPILPRQLNPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTIELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHEDGGFVVGFVRDEKRETSELVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP

Homology

[Close]

BLAST of MC07g0528 vs. ExPASy Swiss-Prot
Match: Q9LRM7 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED6 PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 1.6e-204
Identity = 346/565 (61.24%), Postives = 438/565 (77.52%), Query Frame = 0


Query: 10  PPPLFRSSSSKVLVDP-TIGTLPIRKLELSPPPPETVSSFPLPILPRQLNPFQKLAESLL 69
           P P   + S ++L++P  I TLP    +L+ P P  V     P  P  LN  QKLA ++L
Sbjct: 22  PQPKNANISRRILINPFKIPTLP----DLTSPVPSPVKL--KPTYP-NLNLLQKLAATML 81

Query: 70  DAVEALLVV-----EKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRGVYLRN 129
           D +E+ +V+       LPK  DPAV +SGNF+PV E PV++ LEVVG+ P+CL+GVY+RN
Sbjct: 82  DKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRN 141

Query: 130 GANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVFPKPIG 189
           GANPM  PL+GHHLFDGDGM+HAV +   N+ SYSCR+T+T+RL QE ALGR+VFPKPIG
Sbjct: 142 GANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIG 201

Query: 190 ELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHVKIMDD 249
           ELHGH GLARL +F AR+GIGL+DG++G GVAN G+V+FNGRLLA+SEDDLPY VKI   
Sbjct: 202 ELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQ 261

Query: 250 GDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGKKSRDV 309
           GDLETIGRF F+DQ++  +IAHPKVD   GDLH LSYN++KKP+L+YL+F+  GKK+RDV
Sbjct: 262 GDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDV 321

Query: 310 AITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSDVD 369
            ITL E TMIHDFAITEN VVIPD Q+VFKLSEM+RGGSPV++   K +RFG+L K D+ 
Sbjct: 322 EITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLT 381

Query: 370 EEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTIELTEI 429
             +I W+DVPDCFCFHLWNAWEE   +G+  +VVIGSCM+PPD+IF++      +EL+EI
Sbjct: 382 GSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEI 441

Query: 430 RMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVDLKTGK 489
           R+N+RT+E  R+V  +G+NLEAG +NR ++GR+++FVY+AIA+PWPKCSGIAKVD++ G 
Sbjct: 442 RLNMRTKESNRKVIVTGVNLEAGHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGT 501

Query: 490 VEKFLYGDGRYGGEPFFVAENGSGGGDHEDGGFVVGFVRDEKRETSELVVVEAAEMKEVA 549
           V +F YG  R+GGEP FV E    G   ED G+V+GFVRDE+++ SE VVV+A +MK+VA
Sbjct: 502 VSEFNYGPSRFGGEPCFVPE----GEGEEDKGYVMGFVRDEEKDESEFVVVDATDMKQVA 561

Query: 550 AVRLPARVPYGFHGTFVSEEDLKGQ 569
           AVRLP RVPYGFHGTFVSE  LK Q
Sbjct: 562 AVRLPERVPYGFHGTFVSENQLKEQ 575

[Close]

BLAST of MC07g0528 vs. ExPASy Swiss-Prot
Match: O24592 (9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays OX=4577 GN=VP14 PE=1 SV=2)

HSP 1 Score: 654.1 bits (1686), Expect = 1.5e-186
Identity = 321/525 (61.14%), Postives = 386/525 (73.52%), Query Frame = 0


Query: 55  RQLNPFQKLAESLLDAVEALLVV------EKLPKTVDPAVHISGNFSPVPEFPVRHDLEV 114
           +QLN FQ+ A + LDA E   V         LP T DPAV I+GNF+PV E P  H+L V
Sbjct: 82  KQLNLFQRAAAAALDAFEEGFVANVLERPHGLPSTADPAVQIAGNFAPVGERPPVHELPV 141

Query: 115 VGEFPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLE 174
            G  P  + GVY RNGANP   P++GHHLFDGDGMVHA+++  G   SY+CRFT T+RL 
Sbjct: 142 SGRIPPFIDGVYARNGANPCFDPVAGHHLFDGDGMVHALRIRNGAAESYACRFTETARLR 201

Query: 175 QEAALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLA 234
           QE A+GR VFPK IGELHGH G+ARL +F AR+  GL+D S GTGVAN GLVYFNGRLLA
Sbjct: 202 QERAIGRPVFPKAIGELHGHSGIARLALFYARAACGLVDPSAGTGVANAGLVYFNGRLLA 261

Query: 235 LSEDDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYL 294
           +SEDDLPYHV++ DDGDLET+GR+ F+ QL C MIAHPK+DP  G+LH LSY+++K+PYL
Sbjct: 262 MSEDDLPYHVRVADDGDLETVGRYDFDGQLGCAMIAHPKLDPATGELHALSYDVIKRPYL 321

Query: 295 KYLRFDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDP 354
           KY  F  DG KS DV I L + TMIHDFAITEN VV+PDHQVVFKL EM+RGGSPVV D 
Sbjct: 322 KYFYFRPDGTKSDDVEIPLEQPTMIHDFAITENLVVVPDHQVVFKLQEMLRGGSPVVLDK 381

Query: 355 NKTSRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSI 414
            KTSRFG+LPK   D  E+ W+DVPDCFCFHLWNAWE+ A  GE  VVVIGSCM P DSI
Sbjct: 382 EKTSRFGVLPKHAADASEMAWVDVPDCFCFHLWNAWEDEA-TGE--VVVIGSCMTPADSI 441

Query: 415 FNDRDRALTIELTEIRMNIRTREVTRRVF---GSGLNLEAGQVNRRFIGRRTRFVYLAIA 474
           FN+ D  L   LTEIR++ RT   TRR        +NLE G VNR  +GR TR+ YLA+A
Sbjct: 442 FNESDERLESVLTEIRLDARTGRSTRRAVLPPSQQVNLEVGMVNRNLLGRETRYAYLAVA 501

Query: 475 EPWPKCSGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDH-EDGGFVVGFVRDE 534
           EPWPK SG AKVDL TG++ KF YG+GR+GGEP FV  + +      ED G+V+ FV DE
Sbjct: 502 EPWPKVSGFAKVDLSTGELTKFEYGEGRFGGEPCFVPMDPAAAHPRGEDDGYVLTFVHDE 561

Query: 535 KRETSELVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQA 570
           +  TSEL+VV AA+M+  A V+LP+RVP+GFHGTF++ ++L+ QA
Sbjct: 562 RAGTSELLVVNAADMRLEATVQLPSRVPFGFHGTFITGQELEAQA 603

[Close]

BLAST of MC07g0528 vs. ExPASy Swiss-Prot
Match: Q5MBR5 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED3 PE=2 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 2.0e-186
Identity = 326/546 (59.71%), Postives = 390/546 (71.43%), Query Frame = 0


Query: 40  PPPETVSSFPLPILPR------QLNPFQKLAESLLDAVEALLVV------EKLPKTVDPA 99
           P P   S+  +P  PR      +LN FQ+ A   LDA E   V         LP T DPA
Sbjct: 66  PSPSGKSAIGVPKAPRKGEEGKRLNFFQRAAAMALDAFEEGFVANVLERPHGLPSTADPA 125

Query: 100 VHISGNFSPVPEFPVRHDLEVVGEFPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAV 159
           V I+GNF+PV E P    L V G  P  + GVY RNGANP   P++GHHLFDGDGMVHAV
Sbjct: 126 VQIAGNFAPVGETPPARALPVSGRIPPFINGVYARNGANPHFDPVAGHHLFDGDGMVHAV 185

Query: 160 KLEAGNKASYSCRFTRTSRLEQEAALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLID 219
           ++  G   SY+CRFT T+RL QE A+GR +FPK IGELHGH G+ARL +F AR+  GL+D
Sbjct: 186 RIRNGAAESYACRFTETARLRQERAMGRPMFPKAIGELHGHSGIARLALFYARAACGLLD 245

Query: 220 GSKGTGVANTGLVYFNGRLLALSEDDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPK 279
            S GTGVAN GL+YFNGRLLA+SEDDLPY V++  DGDLET+GR+ F+ QL C MIAHPK
Sbjct: 246 PSHGTGVANAGLIYFNGRLLAMSEDDLPYQVRVTADGDLETVGRYDFDGQLGCAMIAHPK 305

Query: 280 VDPILGDLHGLSYNMVKKPYLKYLRFDRDGKKSRDVAITLREATMIHDFAITENHVVIPD 339
           +DP  G+LH LSY+++KKPYLKY  F  DG KS DV I L + TMIHDFAITEN+VV+PD
Sbjct: 306 LDPATGELHALSYDVIKKPYLKYFYFAPDGTKSADVEIPLDQPTMIHDFAITENYVVVPD 365

Query: 340 HQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEET 399
           HQVVFKL EM+RGGSPVV D  KTSRFG+LPK   D  E+VW+DVPDCFCFHLWNAWEE 
Sbjct: 366 HQVVFKLQEMLRGGSPVVLDKEKTSRFGVLPKHAADASEMVWVDVPDCFCFHLWNAWEEA 425

Query: 400 AGDGETTVVVIGSCMNPPDSIFNDRDRALTIELTEIRMNIRTREVTRRVF---GSGLNLE 459
             D    VVVIGSCM P DSIFN+ D  L   LTEIR+N RT E TRR      S +NLE
Sbjct: 426 DTD---EVVVIGSCMTPADSIFNESDDRLESVLTEIRLNTRTGESTRRAILPPSSQVNLE 485

Query: 460 AGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAEN 519
            G VNR  +GR+TR+ YLA+AEPWPK SG AKVDL TG++ KF YG+GR+GGEP FV  +
Sbjct: 486 VGMVNRNLLGRKTRYAYLAVAEPWPKVSGFAKVDLATGELTKFEYGEGRFGGEPCFVPMD 545

Query: 520 GSGGGDH-EDGGFVVGFVRDEKRETSELVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEE 570
            +      ED G+++ FV DE+  TSEL+VV AA+M+  A V+LP+RVPYGFHGTF++ +
Sbjct: 546 AAAATPRGEDDGYILSFVHDERAGTSELLVVNAADMRLEATVQLPSRVPYGFHGTFITGD 605

[Close]

BLAST of MC07g0528 vs. ExPASy Swiss-Prot
Match: Q5MBR3 (9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED5 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 6.3e-185
Identity = 332/558 (59.50%), Postives = 389/558 (69.71%), Query Frame = 0


Query: 39  PPPPETVSSFP-------LPILPRQ-------LNPFQKLAESLLDAVEALLVV------E 98
           PPPP     FP            R+       LN FQ+ A   LDA E   +        
Sbjct: 59  PPPPPPTKMFPEAGDAAAAKAAARRCGKKKDGLNFFQRAAAVALDAFEEGFITNVLERPH 118

Query: 99  KLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRGVYLRNGANPMHAPLSGHHLF 158
            LP+T DPAV I+GNF+PV E P    L V G  P  + GVY RNGANP   P +GHHLF
Sbjct: 119 ALPRTADPAVQIAGNFAPVGEQPPVRSLPVSGRIPPFINGVYARNGANPHFEPTAGHHLF 178

Query: 159 DGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVFPKPIGELHGHQGLARLGVFL 218
           DGDGMVHAV++  G   SY+CRFT T+RL QE ALGRAVFPK IGELHGH G+ARL +F 
Sbjct: 179 DGDGMVHAVRIRNGAAESYACRFTETARLGQERALGRAVFPKAIGELHGHSGIARLALFY 238

Query: 219 ARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHVKIMDDGDLETIGRFSFNDQL 278
           AR   GL+D S GTGVAN GLVYFNGRLLA+SEDDLPY V++  DGDLET+GR+ F+ QL
Sbjct: 239 ARGLCGLVDPSHGTGVANAGLVYFNGRLLAMSEDDLPYQVRVTADGDLETVGRYDFDGQL 298

Query: 279 NCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGKKSRDVAITLREATMIHDFAI 338
            C MIAHPK+DP+ G+L  LSY+++KKPYLKY  FD DG KS DV I L + TMIHDFAI
Sbjct: 299 GCAMIAHPKLDPVSGELFALSYDVIKKPYLKYFYFDADGTKSPDVEIELEQPTMIHDFAI 358

Query: 339 TENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSDVDEEEIVWIDVPDCFCF 398
           TEN VV+PDHQVVFKL EM RGGSPVV D  KTSRFG+LPK      E+VW+DVPDCFCF
Sbjct: 359 TENFVVVPDHQVVFKLGEMFRGGSPVVLDREKTSRFGVLPKHATSSLEMVWVDVPDCFCF 418

Query: 399 HLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTIELTEIRMNIRTREVTRRVF- 458
           HLWNAWEE A  GE  VVV+GSCM P DSIFN+ D  L   LTEIR+N RT E TRR   
Sbjct: 419 HLWNAWEE-AESGE--VVVVGSCMTPADSIFNESDEHLESVLTEIRLNTRTGESTRRAVL 478

Query: 459 --GSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVDLKTGKVEKFLYGDGRYG 518
              + +NLE G VNR  +GR+TR+ YLA+AEPWPK SG AKVDL TG++ KF YG+GR+G
Sbjct: 479 PPAAQVNLEVGMVNRAMLGRKTRYAYLAVAEPWPKVSGFAKVDLATGELTKFEYGEGRFG 538

Query: 519 GEPFFVAENGSGGGDH----EDGGFVVGFVRDEKRETSELVVVEAAEMKEVAAVRLPARV 570
           GEP FV   G+G        ED G+++ FVRDE   TSEL+VV AA+M+  A V+LP+RV
Sbjct: 539 GEPCFVPMGGAGAAASPARGEDDGYILSFVRDEAAGTSELLVVNAADMRLEATVQLPSRV 598

[Close]

BLAST of MC07g0528 vs. ExPASy Swiss-Prot
Match: Q9M9F5 (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED9 PE=2 SV=1)

HSP 1 Score: 648.7 bits (1672), Expect = 6.3e-185
Identity = 329/563 (58.44%), Postives = 403/563 (71.58%), Query Frame = 0


Query: 15  RSSSSKVLVDPTIGTLPIRKL--ELSPPPPETVSSFPLPILPRQLNPFQKLAESLLDAVE 74
           +SS S  L  PT      +KL  +++   P+T +  P      +LNP Q+ A  +LDAVE
Sbjct: 107 KSSVSSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQNP------KLNPVQRTAAMVLDAVE 166

Query: 75  ALLVVEKL-----PKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRGVYLRNGANP 134
             ++  +      PKT DPAV I+GNF PVPE PV H+L V G  P C++GVY+RNGANP
Sbjct: 167 NAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANP 226

Query: 135 MHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVFPKPIGELHG 194
           +H P+SGHHLFDGDGMVHAV+ + G+  SY+CRFT T+RL QE   GR VFPK IGELHG
Sbjct: 227 LHKPVSGHHLFDGDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKAIGELHG 286

Query: 195 HQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHVKIMDDGDLE 254
           H G+A+L +F  R   GL+D + G GVAN GLVYFNG LLA+SEDDLPYHVK+   GDLE
Sbjct: 287 HLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLE 346

Query: 255 TIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGKKSRDVAITL 314
           T GR+ F+ QL   MIAHPK+DP   +L  LSY++V KPYLKY RF  DG+KS DV I L
Sbjct: 347 TSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPL 406

Query: 315 REATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSDVDEEEI 374
            + TMIHDFAITEN VVIPD QVVF+L EM+RGGSPVV+D  K SRFGIL K+  D   I
Sbjct: 407 DQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSI 466

Query: 375 VWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTIELTEIRMNI 434
            WI+VPDCFCFHLWN+WEE   D    VVVIGSCM PPDSIFN+ D  L   L+EIR+N+
Sbjct: 467 QWIEVPDCFCFHLWNSWEEPETD---EVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNL 526

Query: 435 RTREVTRR-VFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVDLKTGKVEK 494
           +T E TRR V    +NLEAG VNR  +GR+TR+ YLA+ EPWPK SG AKVDL TG++ K
Sbjct: 527 KTGESTRRPVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRK 586

Query: 495 FLYGDGRYGGEPFFVAENGSGGGDHEDGGFVVGFVRDEKRETSELVVVEAAEMKEVAAVR 554
           ++YG+G+YGGEP F+    SG G+ EDGG+++ FV DE++  SEL ++ A  MK  A V 
Sbjct: 587 YIYGEGKYGGEPLFLP---SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVT 646

Query: 555 LPARVPYGFHGTFVSEEDLKGQA 570
           LP+RVPYGFHGTF+S+EDL  QA
Sbjct: 647 LPSRVPYGFHGTFISKEDLSKQA 655

[Close]

BLAST of MC07g0528 vs. NCBI nr
Match: XP_023548938.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 474/581 (81.58%), Postives = 511/581 (87.95%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLEL----------SPPPPETVSSFPL 60
           MQI LQSP P  L RSSS       T+ T PIRKL+L          SPPPP+ V S   
Sbjct: 1   MQISLQSPLP--LCRSSS-------TVETPPIRKLKLLTLPPPSPPPSPPPPKVVLS--- 60

Query: 61  PILPRQLNPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVG 120
             LPR+LNPFQ LA SLLDAVE+ L+V+KLPKTVDPAV ISGNFSPVPE PVRHDLE++G
Sbjct: 61  -PLPRRLNPFQNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIG 120

Query: 121 EFPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQE 180
             PACLRGVYLRNGANPMHAP+ GHHLFDGDGM+HAV    GN+ASYSCRFTRTSRLEQE
Sbjct: 121 NLPACLRGVYLRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQE 180

Query: 181 AALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALS 240
            ALGR VFPKPIGELHGHQGLARL +FLAR+GIGLID SKGTGVAN GLVYFNGRLLALS
Sbjct: 181 VALGRPVFPKPIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALS 240

Query: 241 EDDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKY 300
           EDDLPYHVKI D GDLETIGRFSFN Q++CPMIAHPKVDPI GDLHGLSYNM+KKPYLKY
Sbjct: 241 EDDLPYHVKIRDGGDLETIGRFSFNSQISCPMIAHPKVDPISGDLHGLSYNMIKKPYLKY 300

Query: 301 LRFDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNK 360
           LRFDR GKK RDV ITLR+ TMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNK
Sbjct: 301 LRFDRLGKKVRDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNK 360

Query: 361 TSRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFN 420
           TSRFGILPKS VDE  IVWIDVPDCFCFHLWNAWEET  +GE TVV +GSCMNPPDSIFN
Sbjct: 361 TSRFGILPKSGVDETGIVWIDVPDCFCFHLWNAWEETGINGEKTVVAVGSCMNPPDSIFN 420

Query: 421 DRDRALTIELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPK 480
           DRD+ALTIELTEIRM++R R+ TRRVFGSG+NLEAGQVNR+ +GRRTRFVY+AIAEPWPK
Sbjct: 421 DRDQALTIELTEIRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPK 480

Query: 481 CSGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGD-HEDGGFVVGFVRDEKRETS 540
           CSGIAKVDL+TGKV+KFLYGDGRYGGEPF+V+ENG+ G D +EDGG+VVGFVRDEKRE S
Sbjct: 481 CSGIAKVDLETGKVKKFLYGDGRYGGEPFYVSENGNSGEDENEDGGYVVGFVRDEKRERS 540

Query: 541 ELVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP 570
           ELVVV+AAEMKEVAAVRLPARVPYGFHGTFVSEE+LKGQAP
Sbjct: 541 ELVVVKAAEMKEVAAVRLPARVPYGFHGTFVSEEELKGQAP 568

[Close]

BLAST of MC07g0528 vs. NCBI nr
Match: KAG6574873.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013444.1 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 940 bits (2430), Expect = 0.0
Identity = 472/580 (81.38%), Postives = 511/580 (88.10%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLELS---------PPPPETVSSFPLP 60
           MQI LQSP P  L RSSS       T+ T PIRKL+L          PPPP+ V S    
Sbjct: 1   MQISLQSPLP--LCRSSS-------TVETTPIRKLKLLTLPPPSPPLPPPPKVVLS---- 60

Query: 61  ILPRQLNPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGE 120
            LPR+LNPFQ LA SLLDAVE+ L+V+KLPKTVDPAV ISGNFSPVPE PVRHDLE++G 
Sbjct: 61  PLPRRLNPFQNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGN 120

Query: 121 FPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEA 180
            PACLRGVYLRNGANPMHAP+ GHHLFDGDGM+HAV    GN+ASYSCRFTRTSRLEQE 
Sbjct: 121 LPACLRGVYLRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEV 180

Query: 181 ALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSE 240
           ALGR VFPKPIGELHGHQGLARL +FLAR+GIGLID SKGTGVAN GLVYFNGRLLALSE
Sbjct: 181 ALGRPVFPKPIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSE 240

Query: 241 DDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYL 300
           DDLPYHVKI D GDLETIGRFSFN Q++CPMIAHPKVDP+ GDLHGLSYNM+KKPYLKYL
Sbjct: 241 DDLPYHVKIRDGGDLETIGRFSFNSQISCPMIAHPKVDPVSGDLHGLSYNMIKKPYLKYL 300

Query: 301 RFDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKT 360
           RFDR GKK RDV ITLR+ TMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKT
Sbjct: 301 RFDRLGKKVRDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKT 360

Query: 361 SRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFND 420
           SRFGILPKS VDE  IVWIDVPDCFCFHLWNAWEET  +GE TVV +GSCMNPPDSIFND
Sbjct: 361 SRFGILPKSGVDETGIVWIDVPDCFCFHLWNAWEETGTNGEKTVVAVGSCMNPPDSIFND 420

Query: 421 RDRALTIELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKC 480
           RD+ALTIELTEIRM++R R+VTRRVFGSG+NLEAGQVNR+ +GRRTRFVY+AIAEPWPKC
Sbjct: 421 RDQALTIELTEIRMDMRNRKVTRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKC 480

Query: 481 SGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGD-HEDGGFVVGFVRDEKRETSE 540
           SGIAKVDL+TGKV+KFLYGDGRYGGEPF+V+ENG+ G D +EDGG++VGFVRDEKRE SE
Sbjct: 481 SGIAKVDLETGKVKKFLYGDGRYGGEPFYVSENGNSGEDENEDGGYLVGFVRDEKRERSE 540

Query: 541 LVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP 570
           LVVV+AAEMKEVAAVRLPARVPYGFHGTFVSEE+LKGQAP
Sbjct: 541 LVVVKAAEMKEVAAVRLPARVPYGFHGTFVSEEELKGQAP 567

[Close]

BLAST of MC07g0528 vs. NCBI nr
Match: XP_038875554.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Benincasa hispida])

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 469/575 (81.57%), Postives = 512/575 (89.04%), Query Frame = 0


Query: 1   MQICLQSPPP-PPLFRSSSSKVLVDPTIGTLPIRKLELSPPPPETVSSFPLPILP--RQL 60
           MQI LQSPPP P L RSSS       TI T PIRKL++  PPP  +S  P  + P  R+L
Sbjct: 1   MQIYLQSPPPLPSLCRSSS-------TIETPPIRKLKVITPPPPPLS-LPKTVSPPLRRL 60

Query: 61  NPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLR 120
           NPFQ LA SLLDAVE+ L+V+KLPKTVDP V ISGNFSPVPE PVRHDLE++G  PACLR
Sbjct: 61  NPFQNLAASLLDAVESSLIVDKLPKTVDPTVQISGNFSPVPECPVRHDLEIIGNLPACLR 120

Query: 121 GVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAV 180
           GVYLRNGANPMHAP+ GHHLFDGDGM+HAV    GNKASYSCRFTRTSRL+QEAALGR V
Sbjct: 121 GVYLRNGANPMHAPIGGHHLFDGDGMIHAVTFHCGNKASYSCRFTRTSRLQQEAALGRPV 180

Query: 181 FPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYH 240
           FPKPIGELHGHQGLARL +FLAR+GIGLIDGSKGTGV N GLVYFNGRLLALSEDDLPYH
Sbjct: 181 FPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVTNAGLVYFNGRLLALSEDDLPYH 240

Query: 241 VKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDG 300
           VKI DDGDLETIGRF+FN Q+NCPMIAHPKVDPI GDLHGLSYNM+KKPYLKYLRFDR G
Sbjct: 241 VKIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFG 300

Query: 301 KKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGIL 360
           KKSRDV ITL E TMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNK SRFG+L
Sbjct: 301 KKSRDVDITLPEPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKISRFGVL 360

Query: 361 PKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALT 420
           PKS+VDE+ IVW++VP+CFCFHLWNAWEET  + E TVVV+GSCMNPPDSIFNDRD+ L 
Sbjct: 361 PKSEVDEKGIVWMEVPNCFCFHLWNAWEETGDNDEKTVVVLGSCMNPPDSIFNDRDQPLR 420

Query: 421 IELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKV 480
           IELTEIRM+++ R+VTRRVFGSG+NLEAGQVNR  +GRRTRFVY+AIAEPWPKCSGIAKV
Sbjct: 421 IELTEIRMDVKNRKVTRRVFGSGMNLEAGQVNRGLVGRRTRFVYMAIAEPWPKCSGIAKV 480

Query: 481 DLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHE--DGGFVVGFVRDEKRETSELVVVE 540
           DL+TGKV+KFLYGDGRYGGEPF+V+ENG+ GGD+E  DGGFVVGFVRDEKRE SELVVV+
Sbjct: 481 DLETGKVKKFLYGDGRYGGEPFYVSENGNCGGDNENEDGGFVVGFVRDEKRERSELVVVK 540

Query: 541 AAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP 570
           AAEM+EVAAVRLPARVPYGFHGTFVSEE+LKGQAP
Sbjct: 541 AAEMEEVAAVRLPARVPYGFHGTFVSEEELKGQAP 567

[Close]

BLAST of MC07g0528 vs. NCBI nr
Match: XP_023006243.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita maxima])

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 468/579 (80.83%), Postives = 509/579 (87.91%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLEL--------SPPPPETVSSFPLPI 60
           MQI LQSP P  L RSSS       T+ T PIRKL+L        SP PP+ VS+     
Sbjct: 1   MQISLQSPLP--LCRSSS-------TVETPPIRKLKLLTLPPPSPSPSPPKVVST----P 60

Query: 61  LPRQLNPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEF 120
           LP ++NPFQ LA SLLDAVE+ L+V+KLPKTVDPAV ISGNFSPVPE PVRHDLE++G  
Sbjct: 61  LPHRINPFQNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNL 120

Query: 121 PACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAA 180
           PACLRGVYLRNGANPMHAP+ GHHLFDGDGM+HAV    GN+ASYSCRFTRTSRL QE A
Sbjct: 121 PACLRGVYLRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLVQEVA 180

Query: 181 LGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSED 240
           LGR VFPKPIGELHGHQGLARL +FLAR+GIGLID SKGTGVAN GLVYFNGRLLALSED
Sbjct: 181 LGRPVFPKPIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSED 240

Query: 241 DLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLR 300
           DLPYHVKI D GDLETIGRFSFN Q++CPMIAHPKVDPILGDLHGLSYNM+KKPYLKYLR
Sbjct: 241 DLPYHVKIRDGGDLETIGRFSFNSQISCPMIAHPKVDPILGDLHGLSYNMIKKPYLKYLR 300

Query: 301 FDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTS 360
           FDR GKK RDV ITLR+ TMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFD NKTS
Sbjct: 301 FDRLGKKVRDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDSNKTS 360

Query: 361 RFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDR 420
           RFGILPKS VDE  IVWIDVPDCFCFHLWNAWEET  +GE TVV +GSCMNPPDSIFNDR
Sbjct: 361 RFGILPKSGVDETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDR 420

Query: 421 DRALTIELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCS 480
           D+ALTIELTEIRM++R R+ TRRVFGSG+NLEAGQVNR+ +GRRTRFVY+AIAEPWPKCS
Sbjct: 421 DQALTIELTEIRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCS 480

Query: 481 GIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGD-HEDGGFVVGFVRDEKRETSEL 540
           GIAKVDL+TGKV+KFLYGDGR+GGEPF+V+ENG+ G D +EDGG+VVGFVRDEKR+ SEL
Sbjct: 481 GIAKVDLETGKVKKFLYGDGRFGGEPFYVSENGNSGEDENEDGGYVVGFVRDEKRQRSEL 540

Query: 541 VVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP 570
           VVV+AAEMKEVAAVRLPARVPYGFHGTFVSEE+LKGQAP
Sbjct: 541 VVVKAAEMKEVAAVRLPARVPYGFHGTFVSEEELKGQAP 566

[Close]

BLAST of MC07g0528 vs. NCBI nr
Match: XP_022959281.1 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita moschata])

HSP 1 Score: 931 bits (2407), Expect = 0.0
Identity = 468/578 (80.97%), Postives = 506/578 (87.54%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLEL-------SPPPPETVSSFPLPIL 60
           MQI LQSP P  L RSSS       T+ T PIRKL+L        PPPP  V   PLP  
Sbjct: 1   MQISLQSPLP--LCRSSS-------TVETPPIRKLKLLTLPPPSPPPPPPKVVLSPLP-- 60

Query: 61  PRQLNPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFP 120
            R+LNPFQ LA SLLDAVE+ L+V+KLPKTVDPAV ISGNFSPVPE PVRHDLE++G  P
Sbjct: 61  -RRLNPFQNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLP 120

Query: 121 ACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAAL 180
           ACLRGVYLRNGANPMHAP+ GHHLFDGDGM+HAV    GN+ASYSCRFTRTSRLEQE AL
Sbjct: 121 ACLRGVYLRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVAL 180

Query: 181 GRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDD 240
           GR VFPKPIGELHGHQGLARL +FLAR+GIGLID SKGTGVAN GLVYFNGRLLALSEDD
Sbjct: 181 GRPVFPKPIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDD 240

Query: 241 LPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRF 300
           LPYHV I D GDLETIGRFSFN Q++CPMIAHPKVDP+ GDLHGLSYNM+KKPYLKYLRF
Sbjct: 241 LPYHVLIRDGGDLETIGRFSFNSQISCPMIAHPKVDPVSGDLHGLSYNMIKKPYLKYLRF 300

Query: 301 DRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSR 360
           DR G+K RDV ITLR+ TMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSR
Sbjct: 301 DRLGRKVRDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSR 360

Query: 361 FGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRD 420
           FGILPKS VDE  IVWIDVPDCFCFHLWNAWEET  +GE TVV +GSCMNPPDSIFNDRD
Sbjct: 361 FGILPKSGVDETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDRD 420

Query: 421 RALTIELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSG 480
           +ALTIELTEIRM++R R+ TRRVFGSG+NLEAGQVNR+ +GRRTRFVY+AIAEPWPKCSG
Sbjct: 421 QALTIELTEIRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSG 480

Query: 481 IAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGD-HEDGGFVVGFVRDEKRETSELV 540
           IAKVDL+T KV+KFLYGDGRYGGEPF+V+ENG+ G D +EDGG+VVGFVRDEKRE SELV
Sbjct: 481 IAKVDLETRKVKKFLYGDGRYGGEPFYVSENGNSGEDENEDGGYVVGFVRDEKRERSELV 540

Query: 541 VVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP 570
           VV+AAEM EVAAVRLPARVPYGFHGTFVSEE+LKGQAP
Sbjct: 541 VVKAAEMMEVAAVRLPARVPYGFHGTFVSEEELKGQAP 566

[Close]

BLAST of MC07g0528 vs. ExPASy TrEMBL
Match: A0A6J1L4D2 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499033 PE=3 SV=1)

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 468/579 (80.83%), Postives = 509/579 (87.91%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLEL--------SPPPPETVSSFPLPI 60
           MQI LQSP P  L RSSS       T+ T PIRKL+L        SP PP+ VS+     
Sbjct: 1   MQISLQSPLP--LCRSSS-------TVETPPIRKLKLLTLPPPSPSPSPPKVVST----P 60

Query: 61  LPRQLNPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEF 120
           LP ++NPFQ LA SLLDAVE+ L+V+KLPKTVDPAV ISGNFSPVPE PVRHDLE++G  
Sbjct: 61  LPHRINPFQNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNL 120

Query: 121 PACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAA 180
           PACLRGVYLRNGANPMHAP+ GHHLFDGDGM+HAV    GN+ASYSCRFTRTSRL QE A
Sbjct: 121 PACLRGVYLRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLVQEVA 180

Query: 181 LGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSED 240
           LGR VFPKPIGELHGHQGLARL +FLAR+GIGLID SKGTGVAN GLVYFNGRLLALSED
Sbjct: 181 LGRPVFPKPIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSED 240

Query: 241 DLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLR 300
           DLPYHVKI D GDLETIGRFSFN Q++CPMIAHPKVDPILGDLHGLSYNM+KKPYLKYLR
Sbjct: 241 DLPYHVKIRDGGDLETIGRFSFNSQISCPMIAHPKVDPILGDLHGLSYNMIKKPYLKYLR 300

Query: 301 FDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTS 360
           FDR GKK RDV ITLR+ TMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFD NKTS
Sbjct: 301 FDRLGKKVRDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDSNKTS 360

Query: 361 RFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDR 420
           RFGILPKS VDE  IVWIDVPDCFCFHLWNAWEET  +GE TVV +GSCMNPPDSIFNDR
Sbjct: 361 RFGILPKSGVDETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDR 420

Query: 421 DRALTIELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCS 480
           D+ALTIELTEIRM++R R+ TRRVFGSG+NLEAGQVNR+ +GRRTRFVY+AIAEPWPKCS
Sbjct: 421 DQALTIELTEIRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCS 480

Query: 481 GIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGD-HEDGGFVVGFVRDEKRETSEL 540
           GIAKVDL+TGKV+KFLYGDGR+GGEPF+V+ENG+ G D +EDGG+VVGFVRDEKR+ SEL
Sbjct: 481 GIAKVDLETGKVKKFLYGDGRFGGEPFYVSENGNSGEDENEDGGYVVGFVRDEKRQRSEL 540

Query: 541 VVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP 570
           VVV+AAEMKEVAAVRLPARVPYGFHGTFVSEE+LKGQAP
Sbjct: 541 VVVKAAEMKEVAAVRLPARVPYGFHGTFVSEEELKGQAP 566

[Close]

BLAST of MC07g0528 vs. ExPASy TrEMBL
Match: A0A6J1H4F0 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460311 PE=3 SV=1)

HSP 1 Score: 931 bits (2407), Expect = 0.0
Identity = 468/578 (80.97%), Postives = 506/578 (87.54%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLEL-------SPPPPETVSSFPLPIL 60
           MQI LQSP P  L RSSS       T+ T PIRKL+L        PPPP  V   PLP  
Sbjct: 1   MQISLQSPLP--LCRSSS-------TVETPPIRKLKLLTLPPPSPPPPPPKVVLSPLP-- 60

Query: 61  PRQLNPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFP 120
            R+LNPFQ LA SLLDAVE+ L+V+KLPKTVDPAV ISGNFSPVPE PVRHDLE++G  P
Sbjct: 61  -RRLNPFQNLAASLLDAVESSLIVDKLPKTVDPAVQISGNFSPVPECPVRHDLEIIGNLP 120

Query: 121 ACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAAL 180
           ACLRGVYLRNGANPMHAP+ GHHLFDGDGM+HAV    GN+ASYSCRFTRTSRLEQE AL
Sbjct: 121 ACLRGVYLRNGANPMHAPIGGHHLFDGDGMIHAVTFHGGNEASYSCRFTRTSRLEQEVAL 180

Query: 181 GRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDD 240
           GR VFPKPIGELHGHQGLARL +FLAR+GIGLID SKGTGVAN GLVYFNGRLLALSEDD
Sbjct: 181 GRPVFPKPIGELHGHQGLARLAIFLARAGIGLIDSSKGTGVANAGLVYFNGRLLALSEDD 240

Query: 241 LPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRF 300
           LPYHV I D GDLETIGRFSFN Q++CPMIAHPKVDP+ GDLHGLSYNM+KKPYLKYLRF
Sbjct: 241 LPYHVLIRDGGDLETIGRFSFNSQISCPMIAHPKVDPVSGDLHGLSYNMIKKPYLKYLRF 300

Query: 301 DRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSR 360
           DR G+K RDV ITLR+ TMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSR
Sbjct: 301 DRLGRKVRDVDITLRDPTMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSR 360

Query: 361 FGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRD 420
           FGILPKS VDE  IVWIDVPDCFCFHLWNAWEET  +GE TVV +GSCMNPPDSIFNDRD
Sbjct: 361 FGILPKSGVDETGIVWIDVPDCFCFHLWNAWEETGSNGEKTVVAVGSCMNPPDSIFNDRD 420

Query: 421 RALTIELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSG 480
           +ALTIELTEIRM++R R+ TRRVFGSG+NLEAGQVNR+ +GRRTRFVY+AIAEPWPKCSG
Sbjct: 421 QALTIELTEIRMDMRNRKATRRVFGSGMNLEAGQVNRKLVGRRTRFVYMAIAEPWPKCSG 480

Query: 481 IAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGD-HEDGGFVVGFVRDEKRETSELV 540
           IAKVDL+T KV+KFLYGDGRYGGEPF+V+ENG+ G D +EDGG+VVGFVRDEKRE SELV
Sbjct: 481 IAKVDLETRKVKKFLYGDGRYGGEPFYVSENGNSGEDENEDGGYVVGFVRDEKRERSELV 540

Query: 541 VVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQAP 570
           VV+AAEM EVAAVRLPARVPYGFHGTFVSEE+LKGQAP
Sbjct: 541 VVKAAEMMEVAAVRLPARVPYGFHGTFVSEEELKGQAP 566

[Close]

BLAST of MC07g0528 vs. ExPASy TrEMBL
Match: A0A0A0LIE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G160620 PE=3 SV=1)

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 455/574 (79.27%), Postives = 507/574 (88.33%), Query Frame = 0


Query: 1   MQICLQSPPPP-PLFRSSSSKVLVDPTIGTLPIRKLELSPPPPETVSSFPLPILP--RQL 60
           MQI LQSPPPP  L RSSS       TI T PIRKL++  PP   +S  P  + P  R+L
Sbjct: 1   MQISLQSPPPPLTLCRSSS-------TIETPPIRKLKVITPPRPPLS-LPKKVSPPQRRL 60

Query: 61  NPFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLR 120
           NP QK A SLLDAVE+ L V+KLPKT+DPAV ISGNFSPVPE  VRHDLE++G  PACLR
Sbjct: 61  NPLQKFAASLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLR 120

Query: 121 GVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAV 180
           G+YLRNGANPMHAP  GHHLFDGDGM+HAV   +GNKASYSCRFTRT+RL+QEAALGR V
Sbjct: 121 GIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLV 180

Query: 181 FPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYH 240
           FPKPIGELHGHQGLARL +FLAR+GIGLIDGSKGTGVAN GLVYFNGRLLALSEDDLPYH
Sbjct: 181 FPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYH 240

Query: 241 VKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDG 300
           V+I DDGDLETIGRF+FN Q+NCPMIAHPKVDPI GDLHGLSYNM+KKPYLKYLRFDR G
Sbjct: 241 VQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFG 300

Query: 301 KKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGIL 360
           KKSRDV ITLRE TMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGIL
Sbjct: 301 KKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGIL 360

Query: 361 PKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALT 420
           PKS VDE+ IVWI+VP+CFCFHLWNAWEET G+ E ++VV+GSCMNPPDSIFNDRD+ L 
Sbjct: 361 PKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLR 420

Query: 421 IELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKV 480
           IELTEIRM++++R+VTRRVFGSG+NLEAGQ+NR  +GR+TRFVY+AIA+PWPKCSGIAKV
Sbjct: 421 IELTEIRMDVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKV 480

Query: 481 DLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHE--DGGFVVGFVRDEKRETSELVVVE 540
           DL+TGKV+KFLYG+GRYGGEPF+V ENG+ GGD+E  DGG++VGFVRDEKRE SE+VVV+
Sbjct: 481 DLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVK 540

Query: 541 AAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQA 569
           AAEM+EVAAVRLP RVPYGFHGTFVSEE+L GQA
Sbjct: 541 AAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 566

[Close]

BLAST of MC07g0528 vs. ExPASy TrEMBL
Match: A0A5D3CZW1 (9-cis-epoxycarotenoid dioxygenase NCED6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold96G001030 PE=3 SV=1)

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 454/573 (79.23%), Postives = 500/573 (87.26%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLELSPPPPETVSSFPLPILP--RQLN 60
           MQI L SP PP     SSS      TI T PIRKL++  PP   +S  P  + P  RQLN
Sbjct: 1   MQISLLSPTPPLTPCRSSS------TIETPPIRKLKVITPPHPPLS-LPKKVSPPLRQLN 60

Query: 61  PFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRG 120
           P Q  A SLLDAVE+ L V+KLPKTVDPAV ISGNFSPVPE  VRHDLE++G  PACLRG
Sbjct: 61  PLQNFAASLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG 120

Query: 121 VYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVF 180
           VYLRNGANPMHAP  GHHLFDGDGM+HAV   + NKASYSCRFTRT+RL+ EAALGR VF
Sbjct: 121 VYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVF 180

Query: 181 PKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHV 240
           PKPIGELHGHQGLARL +FLAR+GIG+IDGSKGTGVAN GLVYFNGRLLALSEDDLPYHV
Sbjct: 181 PKPIGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHV 240

Query: 241 KIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGK 300
           +I DDGDLETIGRF FN Q+NCPMIAHPKVDPI GDLHGLSYNM+KKPYLKYLRFD  GK
Sbjct: 241 QIKDDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGK 300

Query: 301 KSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILP 360
           KSRDV ITLRE TMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILP
Sbjct: 301 KSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILP 360

Query: 361 KSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTI 420
           KS VDE+ IVWI+VP+CFCFHLWNAWEET G+ E ++VVIGSCMNPPDSIFNDRD+ L I
Sbjct: 361 KSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRI 420

Query: 421 ELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVD 480
           ELTEIRM+++ R+VTRRVFGSG+NLEAGQ+NRRF+GR+TRFVY+AIAEPWPKCSGIAKVD
Sbjct: 421 ELTEIRMDVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVD 480

Query: 481 LKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHE--DGGFVVGFVRDEKRETSELVVVEA 540
           L+TGKV+KFLYG+GRYGGEPF+V+ENG+ GGD+E  DGGF+VGFVRDEKRE SE+VVV+A
Sbjct: 481 LETGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKA 540

Query: 541 AEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQA 569
           AEM+EVAAVRLP RVPYGFHGTFVSEE+L GQA
Sbjct: 541 AEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 566

[Close]

BLAST of MC07g0528 vs. ExPASy TrEMBL
Match: A0A1S3CD31 (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499487 PE=3 SV=1)

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 454/573 (79.23%), Postives = 500/573 (87.26%), Query Frame = 0


Query: 1   MQICLQSPPPPPLFRSSSSKVLVDPTIGTLPIRKLELSPPPPETVSSFPLPILP--RQLN 60
           MQI L SP PP     SSS      TI T PIRKL++  PP   +S  P  + P  RQLN
Sbjct: 1   MQISLLSPTPPLTPCRSSS------TIETPPIRKLKVITPPHPPLS-LPKKVSPPLRQLN 60

Query: 61  PFQKLAESLLDAVEALLVVEKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRG 120
           P Q  A SLLDAVE+ L V+KLPKTVDPAV ISGNFSPVPE  VRHDLE++G  PACLRG
Sbjct: 61  PLQNFAASLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG 120

Query: 121 VYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVF 180
           VYLRNGANPMHAP  GHHLFDGDGM+HAV   + NKASYSCRFTRT+RL+ EAALGR VF
Sbjct: 121 VYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVF 180

Query: 181 PKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHV 240
           PKPIGELHGHQGLARL +FLAR+GIG+IDGSKGTGVAN GLVYFNGRLLALSEDDLPYHV
Sbjct: 181 PKPIGELHGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHV 240

Query: 241 KIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGK 300
           +I DDGDLETIGRF FN Q+NCPMIAHPKVDPI GDLHGLSYNM+KKPYLKYLRFD  GK
Sbjct: 241 QIKDDGDLETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGK 300

Query: 301 KSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILP 360
           KSRDV ITLRE TMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDP KTSRFGILP
Sbjct: 301 KSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILP 360

Query: 361 KSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTI 420
           KS VDE+ IVWI+VP+CFCFHLWNAWEET G+ E ++VVIGSCMNPPDSIFNDRD+ L I
Sbjct: 361 KSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRI 420

Query: 421 ELTEIRMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVD 480
           ELTEIRM+++ R+VTRRVFGSG+NLEAGQ+NRRF+GR+TRFVY+AIAEPWPKCSGIAKVD
Sbjct: 421 ELTEIRMDVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVD 480

Query: 481 LKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHE--DGGFVVGFVRDEKRETSELVVVEA 540
           L+TGKV+KFLYG+GRYGGEPF+V+ENG+ GGD+E  DGGF+VGFVRDEKRE SE+VVV+A
Sbjct: 481 LETGKVKKFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKA 540

Query: 541 AEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQA 569
           AEM+EVAAVRLP RVPYGFHGTFVSEE+L GQA
Sbjct: 541 AEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 566

[Close]

BLAST of MC07g0528 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 713.8 bits (1841), Expect = 1.1e-205
Identity = 346/565 (61.24%), Postives = 438/565 (77.52%), Query Frame = 0


Query: 10  PPPLFRSSSSKVLVDP-TIGTLPIRKLELSPPPPETVSSFPLPILPRQLNPFQKLAESLL 69
           P P   + S ++L++P  I TLP    +L+ P P  V     P  P  LN  QKLA ++L
Sbjct: 22  PQPKNANISRRILINPFKIPTLP----DLTSPVPSPVKL--KPTYP-NLNLLQKLAATML 81

Query: 70  DAVEALLVV-----EKLPKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRGVYLRN 129
           D +E+ +V+       LPK  DPAV +SGNF+PV E PV++ LEVVG+ P+CL+GVY+RN
Sbjct: 82  DKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRN 141

Query: 130 GANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVFPKPIG 189
           GANPM  PL+GHHLFDGDGM+HAV +   N+ SYSCR+T+T+RL QE ALGR+VFPKPIG
Sbjct: 142 GANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIG 201

Query: 190 ELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHVKIMDD 249
           ELHGH GLARL +F AR+GIGL+DG++G GVAN G+V+FNGRLLA+SEDDLPY VKI   
Sbjct: 202 ELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQ 261

Query: 250 GDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGKKSRDV 309
           GDLETIGRF F+DQ++  +IAHPKVD   GDLH LSYN++KKP+L+YL+F+  GKK+RDV
Sbjct: 262 GDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDV 321

Query: 310 AITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSDVD 369
            ITL E TMIHDFAITEN VVIPD Q+VFKLSEM+RGGSPV++   K +RFG+L K D+ 
Sbjct: 322 EITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLT 381

Query: 370 EEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTIELTEI 429
             +I W+DVPDCFCFHLWNAWEE   +G+  +VVIGSCM+PPD+IF++      +EL+EI
Sbjct: 382 GSDINWVDVPDCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEI 441

Query: 430 RMNIRTREVTRRVFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVDLKTGK 489
           R+N+RT+E  R+V  +G+NLEAG +NR ++GR+++FVY+AIA+PWPKCSGIAKVD++ G 
Sbjct: 442 RLNMRTKESNRKVIVTGVNLEAGHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGT 501

Query: 490 VEKFLYGDGRYGGEPFFVAENGSGGGDHEDGGFVVGFVRDEKRETSELVVVEAAEMKEVA 549
           V +F YG  R+GGEP FV E    G   ED G+V+GFVRDE+++ SE VVV+A +MK+VA
Sbjct: 502 VSEFNYGPSRFGGEPCFVPE----GEGEEDKGYVMGFVRDEEKDESEFVVVDATDMKQVA 561

Query: 550 AVRLPARVPYGFHGTFVSEEDLKGQ 569
           AVRLP RVPYGFHGTFVSE  LK Q
Sbjct: 562 AVRLPERVPYGFHGTFVSENQLKEQ 575

[Close]

BLAST of MC07g0528 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 648.7 bits (1672), Expect = 4.5e-186
Identity = 329/563 (58.44%), Postives = 403/563 (71.58%), Query Frame = 0


Query: 15  RSSSSKVLVDPTIGTLPIRKL--ELSPPPPETVSSFPLPILPRQLNPFQKLAESLLDAVE 74
           +SS S  L  PT      +KL  +++   P+T +  P      +LNP Q+ A  +LDAVE
Sbjct: 107 KSSVSSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQNP------KLNPVQRTAAMVLDAVE 166

Query: 75  ALLVVEKL-----PKTVDPAVHISGNFSPVPEFPVRHDLEVVGEFPACLRGVYLRNGANP 134
             ++  +      PKT DPAV I+GNF PVPE PV H+L V G  P C++GVY+RNGANP
Sbjct: 167 NAMISHERRRHPHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANP 226

Query: 135 MHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQEAALGRAVFPKPIGELHG 194
           +H P+SGHHLFDGDGMVHAV+ + G+  SY+CRFT T+RL QE   GR VFPK IGELHG
Sbjct: 227 LHKPVSGHHLFDGDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKAIGELHG 286

Query: 195 HQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLALSEDDLPYHVKIMDDGDLE 254
           H G+A+L +F  R   GL+D + G GVAN GLVYFNG LLA+SEDDLPYHVK+   GDLE
Sbjct: 287 HLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLE 346

Query: 255 TIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLKYLRFDRDGKKSRDVAITL 314
           T GR+ F+ QL   MIAHPK+DP   +L  LSY++V KPYLKY RF  DG+KS DV I L
Sbjct: 347 TSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPL 406

Query: 315 REATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPNKTSRFGILPKSDVDEEEI 374
            + TMIHDFAITEN VVIPD QVVF+L EM+RGGSPVV+D  K SRFGIL K+  D   I
Sbjct: 407 DQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSI 466

Query: 375 VWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIFNDRDRALTIELTEIRMNI 434
            WI+VPDCFCFHLWN+WEE   D    VVVIGSCM PPDSIFN+ D  L   L+EIR+N+
Sbjct: 467 QWIEVPDCFCFHLWNSWEEPETD---EVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNL 526

Query: 435 RTREVTRR-VFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEPWPKCSGIAKVDLKTGKVEK 494
           +T E TRR V    +NLEAG VNR  +GR+TR+ YLA+ EPWPK SG AKVDL TG++ K
Sbjct: 527 KTGESTRRPVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRK 586

Query: 495 FLYGDGRYGGEPFFVAENGSGGGDHEDGGFVVGFVRDEKRETSELVVVEAAEMKEVAAVR 554
           ++YG+G+YGGEP F+    SG G+ EDGG+++ FV DE++  SEL ++ A  MK  A V 
Sbjct: 587 YIYGEGKYGGEPLFLP---SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVT 646

Query: 555 LPARVPYGFHGTFVSEEDLKGQA 570
           LP+RVPYGFHGTF+S+EDL  QA
Sbjct: 647 LPSRVPYGFHGTFISKEDLSKQA 655

[Close]

BLAST of MC07g0528 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 646.0 bits (1665), Expect = 2.9e-185
Identity = 335/584 (57.36%), Postives = 408/584 (69.86%), Query Frame = 0


Query: 9   PPPPLFRSSSSKVLVDPTIGTLPIRKLELS-----PP----PPETVSSFPLPILP----- 68
           PP    +SS         + +   RKL +S     PP    P ++ +S  + + P     
Sbjct: 22  PPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQSSNSPAIVVKPKAKES 81

Query: 69  --RQLNPFQKLAESLLDAVEALLV----VEKLPKTVDPAVHISGNFSPVPEFPVRHDLEV 128
             +Q+N FQ+ A + LDA E  LV    +  LPKT DP+V I+GNF+PV E PVR +L V
Sbjct: 82  NTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPV 141

Query: 129 VGEFPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLE 188
           VG+ P  ++GVY+RNGANP+H P++GHH FDGDGMVHAVK E G+ ASY+CRFT+T+R  
Sbjct: 142 VGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGS-ASYACRFTQTNRFV 201

Query: 189 QEAALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLA 248
           QE  LGR VFPK IGELHGH G+ARL +F AR+  G++D + GTGVAN GLVYFNGRLLA
Sbjct: 202 QERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLA 261

Query: 249 LSEDDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYL 308
           +SEDDLPY V+I  +GDL+T+GRF F+ QL   MIAHPKVDP  G+L  LSY++V KPYL
Sbjct: 262 MSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYL 321

Query: 309 KYLRFDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDP 368
           KY RF  DG KS DV I L + TM+HDFAITEN VV+PD QVVFKL EM+RGGSPVV+D 
Sbjct: 322 KYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDK 381

Query: 369 NKTSRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSI 428
           NK +RFGIL K   D   I WID PDCFCFHLWNAWEE   D    VVVIGSCM PPDSI
Sbjct: 382 NKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETD---EVVVIGSCMTPPDSI 441

Query: 429 FNDRDRALTIELTEIRMNIRTREVTRRVFGSG----LNLEAGQVNRRFIGRRTRFVYLAI 488
           FN+ D  L   L+EIR+N++T E TRR   S     +NLEAG VNR  +GR+T+F YLA+
Sbjct: 442 FNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLAL 501

Query: 489 AEPWPKCSGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHEDGGFVVGFVRDE 548
           AEPWPK SG AKVDL TG+V+K LYGD RYGGEP F+     G G  ED G+++ FV DE
Sbjct: 502 AEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFL----PGEGGEEDEGYILCFVHDE 561

Query: 549 KRETSELVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQ 569
           K   SEL +V A  ++  A V+LP+RVPYGFHGTF+  +DL  Q
Sbjct: 562 KTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

[Close]

BLAST of MC07g0528 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 626.3 bits (1614), Expect = 2.4e-179
Identity = 305/521 (58.54%), Postives = 377/521 (72.36%), Query Frame = 0


Query: 54  PRQLNPFQKLAESLLDAVEALLVVEK----LPKTVDPAVHISGNFSPVPEFPVRHDLEVV 113
           P +LN FQK A   +DA E  L+  +    LPKT DP V I+GN+SPVPE  VR +L V 
Sbjct: 66  PLRLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVE 125

Query: 114 GEFPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCRFTRTSRLEQ 173
           G  P C+ GVY+RNGANPM  P +GHHLFDGDGMVHAVK+  G+ ASY+CRFT+T RL Q
Sbjct: 126 GTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGS-ASYACRFTKTERLVQ 185

Query: 174 EAALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLVYFNGRLLAL 233
           E  LGR VFPK IGELHGH G+ARL +F AR   GLI+   G GVAN GLVYFN RLLA+
Sbjct: 186 EKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAM 245

Query: 234 SEDDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSYNMVKKPYLK 293
           SEDDLPY +KI   GDL+T+GR+ F+ QL   MIAHPK+DP+  +LH LSY++VKKPYLK
Sbjct: 246 SEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLK 305

Query: 294 YLRFDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRGGSPVVFDPN 353
           Y RF  DG KS ++ I L   TMIHDFAITEN VVIPD QVVFKL EM+ G SPVVFD  
Sbjct: 306 YFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGE 365

Query: 354 KTSRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGSCMNPPDSIF 413
           K SR GI+PK   +  +I+W++ P+ FCFHLWNAWE    +    +VVIGSCM+P DSIF
Sbjct: 366 KVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETE---EIVVIGSCMSPADSIF 425

Query: 414 NDRDRALTIELTEIRMNIRTREVTRR--VFGSGLNLEAGQVNRRFIGRRTRFVYLAIAEP 473
           N+RD +L   L+EIR+N+RTR+ TRR  +    +NLE G VNR  +GR+TRF +LAIA P
Sbjct: 426 NERDESLRSVLSEIRINLRTRKTTRRSLLVNEDVNLEIGMVNRNRLGRKTRFAFLAIAYP 485

Query: 474 WPKCSGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHEDGGFVVGFVRDEKRE 533
           WPK SG AKVDL TG+++K++YG  +YGGEPFF+  N   G ++ED G++   V DE+ +
Sbjct: 486 WPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETK 545

Query: 534 TSELVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQ 569
           TSEL ++ A  +K  A ++LP+RVPYGFHGTFV   +L  Q
Sbjct: 546 TSELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

[Close]

BLAST of MC07g0528 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 612.8 bits (1579), Expect = 2.7e-175
Identity = 309/532 (58.08%), Postives = 378/532 (71.05%), Query Frame = 0


Query: 49  PLPILPR----QLNPFQKLAESLLDAVEALLV----VEKLPKTVDPAVHISGNFSPVPEF 108
           P PI+P     + NP Q+ A + LD  E  L+     + LPKTVDP   ISGN++PVPE 
Sbjct: 64  PNPIIPEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQ 123

Query: 109 PVRHDLEVVGEFPACLRGVYLRNGANPMHAPLSGHHLFDGDGMVHAVKLEAGNKASYSCR 168
            V+  L V G+ P C+ GVYLRNGANP+  P+SGHHLFDGDGMVHAVK+  G+ ASYSCR
Sbjct: 124 SVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGD-ASYSCR 183

Query: 169 FTRTSRLEQEAALGRAVFPKPIGELHGHQGLARLGVFLARSGIGLIDGSKGTGVANTGLV 228
           FT T RL QE  LG  +FPK IGELHGH G+ARL +F AR   GL++   GTGVAN GLV
Sbjct: 184 FTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLV 243

Query: 229 YFNGRLLALSEDDLPYHVKIMDDGDLETIGRFSFNDQLNCPMIAHPKVDPILGDLHGLSY 288
           YF+ RLLA+SEDDLPY V++ D+GDLETIGRF F+ QL+  MIAHPK+DP+  +L  LSY
Sbjct: 244 YFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSY 303

Query: 289 NMVKKPYLKYLRFDRDGKKSRDVAITLREATMIHDFAITENHVVIPDHQVVFKLSEMVRG 348
           ++VKKPYLKY +F  +G+KS DV I L   TM+HDFAITEN VVIPD QVVFKLS+M  G
Sbjct: 304 DVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLG 363

Query: 349 GSPVVFDPNKTSRFGILPKSDVDEEEIVWIDVPDCFCFHLWNAWEETAGDGETTVVVIGS 408
            SPV +D  K SRFGILP++  D  E+VW++ P+ FCFHLWNAWE    D    VVVIGS
Sbjct: 364 KSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETD---EVVVIGS 423

Query: 409 CMNPPDSIFNDRDRALTIELTEIRMNIRTREVTRRVFGSG---LNLEAGQVNRRFIGRRT 468
           CM P DSIFN+ D  L   L+EIR+N++T + TRR    G   +NLEAG VNR  +GR+T
Sbjct: 424 CMTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKT 483

Query: 469 RFVYLAIAEPWPKCSGIAKVDLKTGKVEKFLYGDGRYGGEPFFVAENGSGGGDHEDGGFV 528
           R+ YLAIAEPWPK SG AKVDL TG+V+   YG  +YGGEPFF+       G  ED G++
Sbjct: 484 RYAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDG--EDDGYI 543

Query: 529 VGFVRDEKRETSELVVVEAAEMKEVAAVRLPARVPYGFHGTFVSEEDLKGQA 570
           + FV DE+   SEL +V A  ++  A V+LP+RVPYGFHGTFV+  D+  QA
Sbjct: 544 MSFVHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589

The following BLAST results are available for this feature:

BLAST of MC07g0528 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LRM7	1.6e-204	61.24	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana O...	[more]
O24592	1.5e-186	61.14	9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays OX=4577 GN=VP14 P...	[more]
Q5MBR5	2.0e-186	59.71	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Oryza sativa subsp. ja...	[more]
Q5MBR3	6.3e-185	59.50	9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Oryza sativa subsp. ja...	[more]
Q9M9F5	6.3e-185	58.44	9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana O...	[more]

BLAST of MC07g0528 vs. NCBI nr


Match Name	Identity	Description
XP_023548938.1	81.58	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita pepo subsp. pe...	[more]
KAG6574873.1	81.38	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic, partial [Cucurbita argyr...	[more]
XP_038875554.1	81.57	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Benincasa hispida]	[more]
XP_023006243.1	80.83	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita maxima]	[more]
XP_022959281.1	80.97	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucurbita moschata]	[more]

BLAST of MC07g0528 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1L4D2	80.83	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita maxima OX=36...	[more]
A0A6J1H4F0	80.97	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucurbita moschata OX=...	[more]
A0A0A0LIE2	79.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G160620 PE=3 SV=1	[more]
A0A5D3CZW1	79.23	9-cis-epoxycarotenoid dioxygenase NCED6 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CD31	79.23	9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Cucumis melo OX=3656 G...	[more]

BLAST of MC07g0528 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24220.1	1.1e-205	61.24	nine-cis-epoxycarotenoid dioxygenase 6	[more]
AT1G78390.1	4.5e-186	58.44	nine-cis-epoxycarotenoid dioxygenase 9	[more]
AT3G14440.1	2.9e-185	57.36	nine-cis-epoxycarotenoid dioxygenase 3	[more]
AT4G18350.1	2.4e-179	58.54	nine-cis-epoxycarotenoid dioxygenase 2	[more]
AT1G30100.1	2.7e-175	58.08	nine-cis-epoxycarotenoid dioxygenase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 99..561 e-value: 4.1E-114 score: 382.3
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 53..568
None	No IPR available	PANTHER	PTHR10543:SF101	9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED6, CHLOROPLASTIC	coord: 53..568

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0528.1	MC07g0528.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
biological_process	GO:0010114	response to red light
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0009535	chloroplast thylakoid membrane
molecular_function	GO:0045549	9-cis-epoxycarotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen