MC07g0523 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0523
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	cytochrome b5-like
Location	MC07: 13249802 .. 13253293 (+)
RNA-Seq Expression	MC07g0523
Synteny	MC07g0523

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGTCTATTAGAATCCTTCTTTTATTAGTATAACAACTTAGGAAAACTTACGACTTCAAAAAGATAAGTATATCAATTACTGATTAACTAGGTTTATATTCGCCGCTAAAATCATTCTTGCCGATTCGAACTTCTATAATTAAATATATCTATTACAATCTCTGCTGTCGGCTGTTTGGGAAAAAAAGCACCACTAGCAAGGCTCACCCACCACCGAATTCAATTGAATGCTTACCATTGCTGCCCACTGGCACAATTTACTCCCTCCCCTTTATTTCACTGGCTCACTCCCATTATGTAGCCCACAGCTTCCCATTCCGCTCCTTGCTTCTTCTTCACCATCGTTTCTCTGCCGTCTGTGGCCAAATACCCAAATGGGTGGTGACGGAAAGATCTTCACCTTGGCGGAAGTTTCTCAGCACAACACCCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTTGATTCATACCCATTTCAGAATCAAGCTGTTTTCTTCTTCCTATCTCTTTGTACTGATGTCTGATTGTGCGTTGAGGAATTTGATTGCAATGGAGTTGTTTTTTCTTGCTCTGTTTTCGTCTGCTCTTGAAATCTGACATGCATTTTGGTTTAGACCCATTTTTCTTCTCTGTTGAATGTGTTTTATCCATGGCTAAGTATTAAACTGCTGCATAATGAACTTTGCCACTCATGAACTGTATAGGGCTGGAGAACGAAGGAATTAAGTTGTGGGTTGTAGTGTGAATCGTACTATTCCTTTACTTTTTTTTTAGGAATGCAAAGAGATTTGATGCTTGAAAAATGGGGAGTGATGTTTCATCTTTACTTAGCTTTAGCTGTTTGGTTCCTAGGTTGCAGTTTTAAGTTAATTGAGTGGCTCTCATTTTAGAAATTGTTAAAGATGAAGTTTGGCTCAAATGCTTCAGTCACTTGGAAAAGAATGAGTGGACAAACATGGATTTGTGTAGCTCATGTTTGGTTTGGGGGTTTTTGATATATAAAAGTTAATATATGGTACAAAAGGCAGGTTTTATAAGCATGAGTATGTGTAACCTAAGTTCATATAACCTATAAGATTAACAACTCAAGTAGTCTAAGATAAACAAACTCACATGACAAACCTGGACTTGAAGTGGAGATATATTATTTGTGGATCTTAAAACTGACCACATTTAGCAATTCGACCGGCTAACTGGAGTCTGAAGCACAACTTAACTGATTAAGACATATATTCTCTGCCAAAATGTTAGAGGTTCGAATTGACCTCACACCTCATGTTGAAGTAAAAAAAGAAAATCAAAAAAAATAAACGGGCCAATATTATACTTCTTGAATAACATTATCCAAAAAAAACTAACCACATTTAGCATTTTGAAATTGAATTGCCTTATTTTGTGTGCTTGACTCCTTGTAGGACCAGGACCAGGATTATGAGGCTTCTTTAGATTTGGCTATCCTTTTTGACAAAAATGTCCTAGTGGATTGTAGTATTGGGGACAGGTTGATCAGAGTAATTCCTATATTTTCTATCAATGAACTTAAGAAAATTGTGTTGTATGTTTTACATCTAAAAGCAGTTTGTTTAAGGAAAAAATGAGCTCCAACTCTTATTGCAAGATCTCATTTTATGTTTGTGTGTGTGAATGCTCTCATTTACTGAGGGCACTAGGACTCTAGTGGGGGTTGGACATCCAACACCTTCTTCAAACTTTACCGATCACGAATCAGGCGTCTAAAGAATGGTCAATGTGCGTCGTTCCCACGCTATCCTGTGGGTTAATGTCGGTGACCGAAACACTTATCTATTATGGAGATTTAGTTCATTCTACTGGTGCCATTTGATACCTTCTTTCTTTGCACCCATAAACCAATCTGAAAGCAACGTTCTGAAGCTTCATGACAGTATGTTTAGTCTCAGTTGAATTTTCATTTTCCATTTTCCGATTGAATTTGCATATTTAGTCTTGGCTAAAATACTTTTATTTTTATTTTTTATTTTTTTTTGCACTCCTTAAACCATGATCACGGTCAACTCCTACTAGGATAGAGTAGAAATCAAGGATTTGTATTGATAATTTACAAACCCAAGTATTCGAGGTACTTGGAACGTTCCTCTTGAGTATTCTCCAGCCCAAATCAATTAAACCATTGTTTTCCTTCACATGGTCCCATTCCCTCCATTTATAACAAATTCATTGGCTAATGAATAATATGTCCTTAATAACCCCAGAATAATATCCTACGAATTATCTTGTCAACCAAATGTAGTAGGGTCAGGCGGTTGACCCATGATTAGTCGAGGTGCACGCAAGCTAGTCCGGACGCACGGATATAAAAGAAAAAAAGTATTCCTAACAAGTAATACACTAATCTTTGGGGAGGTTTAGCCCTACCTAGAATAAACTATAGGAGTCATCAAGAGTTTTAAATTGAAATCTCTTTCTTGACCCACAATGCAAAAAAGGAATGCATGTGTGGGGAGAAAGTCAAGGTGTACTAGCCCCTCAACTCAAGCTTGAGACTTATGACCTTTAGCCAACTCAAGTTTCTCCGAGTGTAGAGTGAGAAGAGAACAGGAGGATAAACACATTGAAAGAGCGTGAAGTGAAAATTGAGAACTTCATCAAGCTCAGTGACATGAATGATATCTTCATAGAATAACACTAGAGGATTACACATTTGTCGTACTATGGTCGGTCGGTTGCCCCTAGATGTCAACAACATGCTTTCCATTGCTTTGGAAGTTGGGTTGGTATTATTAGACTGTAGGAATTCATACAGATTTGTATTTATTTGATATTGATTTTTAGCATGTGTATCATTTTGTTCATCTTGTTATCCCAACAGGTTTATAATGTAACCGAGTTCTTGGAGGACCATCCCGGTGGCGACGAGGTCCTATTGTCAGGCACAGGTAATACATGTTCATGTCGTATAGCTTTTGTTCTGAACTCCGCACACAGTGTTTGTGCGTCGTATACACACTGAAATTTTCGTTGTAAAAGGTAAGGATGCAACTGATGATTTTGAAGATGTGGGTCATAGTGATAGTGCAAGAGAAACAATGAACAAATACTATGTTGGGGAGATCGATATCTCAACGATCCCGGAAAAGATGACATACTCAGCTACAAAGCAGCCTCACTACAATCAGGATAAGACGACCGAGTTCATCGTCAAGCTCCTCCAGTTCCTCGTCCCTCTCGCAATCTTGGGTTTGGCAGTCGGTATCCGCTTTTACACCAAATCAACCTGAGGTTCTTCTTGAGATATTAGGTGGTTTGAGTCTTGTTGTCAATTACGAACTGCTGCATTGTGTTTGTAAAACAAATTTCTATTTTTGTTTTCAAGAATGTTGATTGTATGATTGGTAAAAATTAGTGTTATTTTAGTGATGAAAGTTGTGGGATGTGGAAATATCTGATGGTGTTGAGATTAACTATCAACTTTAGAGAATATTATAGGAGCCTTGTTTACGATCCATG

mRNA sequence

CTTGTCTATTAGAATCCTTCTTTTATTAGTATAACAACTTAGGAAAACTTACGACTTCAAAAAGATAAGTATATCAATTACTGATTAACTAGGTTTATATTCGCCGCTAAAATCATTCTTGCCGATTCGAACTTCTATAATTAAATATATCTATTACAATCTCTGCTGTCGGCTGTTTGGGAAAAAAAGCACCACTAGCAAGGCTCACCCACCACCGAATTCAATTGAATGCTTACCATTGCTGCCCACTGGCACAATTTACTCCCTCCCCTTTATTTCACTGGCTCACTCCCATTATGTAGCCCACAGCTTCCCATTCCGCTCCTTGCTTCTTCTTCACCATCGTTTCTCTGCCGTCTGTGGCCAAATACCCAAATGGGTGGTGACGGAAAGATCTTCACCTTGGCGGAAGTTTCTCAGCACAACACCCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTATAATGTAACCGAGTTCTTGGAGGACCATCCCGGTGGCGACGAGGTCCTATTGTCAGGCACAGGTAAGGATGCAACTGATGATTTTGAAGATGTGGGTCATAGTGATAGTGCAAGAGAAACAATGAACAAATACTATGTTGGGGAGATCGATATCTCAACGATCCCGGAAAAGATGACATACTCAGCTACAAAGCAGCCTCACTACAATCAGGATAAGACGACCGAGTTCATCGTCAAGCTCCTCCAGTTCCTCGTCCCTCTCGCAATCTTGGGTTTGGCAGTCGGTATCCGCTTTTACACCAAATCAACCTGAGGTTCTTCTTGAGATATTAGGTGGTTTGAGTCTTGTTGTCAATTACGAACTGCTGCATTGTGTTTGTAAAACAAATTTCTATTTTTGTTTTCAAGAATGTTGATTGTATGATTGGTAAAAATTAGTGTTATTTTAGTGATGAAAGTTGTGGGATGTGGAAATATCTGATGGTGTTGAGATTAACTATCAACTTTAGAGAATATTATAGGAGCCTTGTTTACGATCCATG

Coding sequence (CDS)

ATGCTTACCATTGCTGCCCACTGGCACAATTTACTCCCTCCCCTTTATTTCACTGGCTCACTCCCATTATGTAGCCCACAGCTTCCCATTCCGCTCCTTGCTTCTTCTTCACCATCGTTTCTCTGCCGTCTGTGGCCAAATACCCAAATGGGTGGTGACGGAAAGATCTTCACCTTGGCGGAAGTTTCTCAGCACAACACCCCCAAGGACTGTTGGTTGATCATCAATGGCAAGGTTTATAATGTAACCGAGTTCTTGGAGGACCATCCCGGTGGCGACGAGGTCCTATTGTCAGGCACAGGTAAGGATGCAACTGATGATTTTGAAGATGTGGGTCATAGTGATAGTGCAAGAGAAACAATGAACAAATACTATGTTGGGGAGATCGATATCTCAACGATCCCGGAAAAGATGACATACTCAGCTACAAAGCAGCCTCACTACAATCAGGATAAGACGACCGAGTTCATCGTCAAGCTCCTCCAGTTCCTCGTCCCTCTCGCAATCTTGGGTTTGGCAGTCGGTATCCGCTTTTACACCAAATCAACCTGA

Protein sequence

MLTIAAHWHNLLPPLYFTGSLPLCSPQLPIPLLASSSPSFLCRLWPNTQMGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAVGIRFYTKST

Homology

[Close]

BLAST of MC07g0523 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.9e-58
Identity = 105/133 (78.95%), Postives = 120/133 (90.23%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MG + KIFTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARE M +YYVGEID +TIP+K+ Y+  KQPHYNQDKT+EFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKS 183
           LGLAVGIR YTKS
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of MC07g0523 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.8e-56
Identity = 102/134 (76.12%), Postives = 115/134 (85.82%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGG  K+FTLAEVS HN  KDCWLII+GKVYNVT+FLEDHPGG EVLLS TGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           D+GHS SAR  +++YYVG+ID STIP K+ Y+  KQPHYNQDKTTEFIVKLLQFLVPL I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 170 LGLAVGIRFYTKST 184
           LG+A G+ FYTK +
Sbjct: 121 LGVAFGVHFYTKQS 134

[Close]

BLAST of MC07g0523 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 6.7e-56
Identity = 100/134 (74.63%), Postives = 117/134 (87.31%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGG+ K+FTLAEVSQHN  KDCWL+I+GKVY+VT+FL+DHPGGDEVLLS TGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS SAR  +++YYVG+ID +TIP K  Y+   QPHYNQDKT+EF+VKLLQFLVPL I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 170 LGLAVGIRFYTKST 184
           LG+A GIRFYTK +
Sbjct: 121 LGVAFGIRFYTKQS 134

[Close]

BLAST of MC07g0523 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.7e-52
Identity = 95/130 (73.08%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 54  GKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGH 113
           GKIFTLAEV+QHN  KDCWLIINGKVY+VT+FLEDHPGGD+VLLS TGKDATDDFED+GH
Sbjct: 2   GKIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGH 61

Query: 114 SDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLA 173
           S SA+  +++YYVG+ID S+IP ++ Y+  KQP YN DKT EF++KLLQFLVPL IL  A
Sbjct: 62  SSSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGA 121

Query: 174 VGIRFYTKST 184
           +GIRFYTKS+
Sbjct: 122 IGIRFYTKSS 131

[Close]

BLAST of MC07g0523 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 7.7e-52
Identity = 95/128 (74.22%), Postives = 110/128 (85.94%), Query Frame = 0


Query: 55  KIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 114
           K++TL EV++HN+  DCWLII GKVYNV++FLEDHPGGD+VLLS TGKDATDDFEDVGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 115 DSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAV 174
            +AR  M++YYVG+ID STIP +  Y   KQPHYNQDKT EFI+K+LQFLVPLAILGLAV
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAV 126

Query: 175 GIRFYTKS 183
            IR YTKS
Sbjct: 127 AIRIYTKS 134

[Close]

BLAST of MC07g0523 vs. NCBI nr
Match: XP_022138846.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 379 bits (972), Expect = 1.22e-132
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MLTIAAHWHNLLPPLYFTGSLPLCSPQLPIPLLASSSPSFLCRLWPNTQMGGDGKIFTLA 60
           MLTIAAHWHNLLPPLYFTGSLPLCSPQLPIPLLASSSPSFLCRLWPNTQMGGDGKIFTLA
Sbjct: 1   MLTIAAHWHNLLPPLYFTGSLPLCSPQLPIPLLASSSPSFLCRLWPNTQMGGDGKIFTLA 60

Query: 61  EVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSDSARET 120
           EVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSDSARET
Sbjct: 61  EVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSDSARET 120

Query: 121 MNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAVGIRFYT 180
           MNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAVGIRFYT
Sbjct: 121 MNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAVGIRFYT 180

Query: 181 KST 183
           KST
Sbjct: 181 KST 183

[Close]

BLAST of MC07g0523 vs. NCBI nr
Match: XP_022959033.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 259 bits (662), Expect = 4.10e-86
Identity = 123/134 (91.79%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGG GK+FTLAEVSQHNT +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARETMNKYYVG+IDISTIPE++TY+A+KQPHYNQDKTTEFIVKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of MC07g0523 vs. NCBI nr
Match: XP_023006481.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 259 bits (661), Expect = 5.82e-86
Identity = 123/134 (91.79%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGG GK+FTLAEVSQHNT +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARE+MNKYYVGEIDISTIPE++TY+A+KQPHYNQDKTTEFIVKLLQFLVPLAI
Sbjct: 61  DVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of MC07g0523 vs. NCBI nr
Match: KAG6574866.1 (Cytochrome b5 isoform B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013438.1 Cytochrome b5 isoform B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258 bits (659), Expect = 1.17e-85
Identity = 122/134 (91.04%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGG GK+FTLAEVSQHNT +DCWLIINGKVYNVTEFLEDHPGGDEVLL+GTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLTGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARETMNKYYVG+IDISTIPE++TY+A+KQPHYNQDKTTEFIVKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of MC07g0523 vs. NCBI nr
Match: XP_004138957.1 (cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cucumis sativus])

HSP 1 Score: 257 bits (656), Expect = 3.36e-85
Identity = 120/134 (89.55%), Postives = 129/134 (96.27%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGGDGK+FT AEVSQHN PKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARETM+KYYVGEIDIST+PE++TY+  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of MC07g0523 vs. ExPASy TrEMBL
Match: A0A6J1CAX1 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1)

HSP 1 Score: 379 bits (972), Expect = 5.90e-133
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0


Query: 1   MLTIAAHWHNLLPPLYFTGSLPLCSPQLPIPLLASSSPSFLCRLWPNTQMGGDGKIFTLA 60
           MLTIAAHWHNLLPPLYFTGSLPLCSPQLPIPLLASSSPSFLCRLWPNTQMGGDGKIFTLA
Sbjct: 1   MLTIAAHWHNLLPPLYFTGSLPLCSPQLPIPLLASSSPSFLCRLWPNTQMGGDGKIFTLA 60

Query: 61  EVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSDSARET 120
           EVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSDSARET
Sbjct: 61  EVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSDSARET 120

Query: 121 MNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAVGIRFYT 180
           MNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAVGIRFYT
Sbjct: 121 MNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAILGLAVGIRFYT 180

Query: 181 KST 183
           KST
Sbjct: 181 KST 183

[Close]

BLAST of MC07g0523 vs. ExPASy TrEMBL
Match: A0A6J1H4S5 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111460140 PE=3 SV=1)

HSP 1 Score: 259 bits (662), Expect = 1.98e-86
Identity = 123/134 (91.79%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGG GK+FTLAEVSQHNT +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARETMNKYYVG+IDISTIPE++TY+A+KQPHYNQDKTTEFIVKLLQFLVPLAI
Sbjct: 61  DVGHSESARETMNKYYVGDIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of MC07g0523 vs. ExPASy TrEMBL
Match: A0A6J1KXV9 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1)

HSP 1 Score: 259 bits (661), Expect = 2.82e-86
Identity = 123/134 (91.79%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGG GK+FTLAEVSQHNT +DCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGHGKVFTLAEVSQHNTRQDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARE+MNKYYVGEIDISTIPE++TY+A+KQPHYNQDKTTEFIVKLLQFLVPLAI
Sbjct: 61  DVGHSESARESMNKYYVGEIDISTIPERLTYTASKQPHYNQDKTTEFIVKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY+KST
Sbjct: 121 LGLAVGIRFYSKST 134

[Close]

BLAST of MC07g0523 vs. ExPASy TrEMBL
Match: A0A0A0LHS9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138200 PE=3 SV=1)

HSP 1 Score: 257 bits (656), Expect = 1.63e-85
Identity = 120/134 (89.55%), Postives = 129/134 (96.27%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGGDGK+FT AEVSQHN PKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLFTFAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARETM+KYYVGEIDIST+PE++TY+  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESARETMSKYYVGEIDISTLPERLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of MC07g0523 vs. ExPASy TrEMBL
Match: A0A5D3CSF5 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G00160 PE=3 SV=1)

HSP 1 Score: 255 bits (651), Expect = 9.41e-85
Identity = 119/134 (88.81%), Postives = 129/134 (96.27%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGGDGK++TLAEVSQHN PKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE
Sbjct: 1   MGGDGKLYTLAEVSQHNNPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARE M+KYY+GEIDISTIPE++TY+  KQPHYNQDKTTEFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMSKYYIGEIDISTIPEQLTYTGPKQPHYNQDKTTEFIIKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKST 183
           LGLAVGIRFY KS+
Sbjct: 121 LGLAVGIRFYNKSS 134

[Close]

BLAST of MC07g0523 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 226.9 bits (577), Expect = 1.3e-59
Identity = 105/133 (78.95%), Postives = 120/133 (90.23%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MG + KIFTL+EVS+HN   DCW++INGKVYNVT+FLEDHPGGD+VLLS TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHS+SARE M +YYVGEID +TIP+K+ Y+  KQPHYNQDKT+EFI+KLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 170 LGLAVGIRFYTKS 183
           LGLAVGIR YTKS
Sbjct: 121 LGLAVGIRIYTKS 133

[Close]

BLAST of MC07g0523 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 196.1 bits (497), Expect = 2.5e-50
Identity = 89/132 (67.42%), Postives = 110/132 (83.33%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           M  D K+ +  EVS+HN  KDCWLII+GKVY+VT F++DHPGGDEVLLS TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQFLVPLAI 169
           DVGHSD+AR+ M+KY++GEID S++P   TY A +QP YNQDKT EFI+K+LQFLVP+ I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 170 LGLAVGIRFYTK 182
           LGLA+ +R YTK
Sbjct: 121 LGLALVVRHYTK 132

[Close]

BLAST of MC07g0523 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 190.7 bits (483), Expect = 1.1e-48
Identity = 85/135 (62.96%), Postives = 115/135 (85.19%), Query Frame = 0


Query: 50  MGGDGKIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFE 109
           MGGDGK+FTL+EVSQH++ KDCW++I+GKVY+VT+FL+DHPGGDEV+L+ TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 110 DVGHSDSARETMNKYYVGEIDISTIPEKMTY--SATKQPHYNQDKTTEFIVKLLQFLVPL 169
           DVGHS +A+  +++YYVG+ID +T+P K  +    + +    QDK+++F++KLLQFLVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 170 AILGLAVGIRFYTKS 183
            ILGLA GIR+YTK+
Sbjct: 121 LILGLAFGIRYYTKT 135

[Close]

BLAST of MC07g0523 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 124.0 bits (310), Expect = 1.2e-28
Identity = 58/126 (46.03%), Postives = 85/126 (67.46%), Query Frame = 0


Query: 56  IFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHSD 115
           + +  +V++H    DCW++I+GKVY+++ F+++HPGGD VLL+ TGKDA+ DFEDV HS 
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 116 SARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTT--EFIVKLLQFLVPLAILGLA 175
            A+E M KY +G++D ST+P    Y    +      +TT  E   KLL +L+PL ILG+A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 176 VGIRFY 180
             +RFY
Sbjct: 124 FALRFY 129

[Close]

BLAST of MC07g0523 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 121.3 bits (303), Expect = 8.0e-28
Identity = 54/127 (42.52%), Postives = 88/127 (69.29%), Query Frame = 0


Query: 55  KIFTLAEVSQHNTPKDCWLIINGKVYNVTEFLEDHPGGDEVLLSGTGKDATDDFEDVGHS 114
           K++++ E + HN   DCW++I+GKVY+V+ ++++HPGGD+VLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 115 DSARETMNKYYVGEIDISTIPEKMTYSATKQPHYNQDKTTEFIVKLLQ-----FLVPLAI 174
             ARE M KY++GE+D S++PE       +   Y +D+  + + KL       ++VP++I
Sbjct: 66  KDARELMEKYFIGELDESSLPE-----IPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 175 LGLAVGI 177
           + ++V +
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:

BLAST of MC07g0523 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48845	1.9e-58	78.95	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49099	1.8e-56	76.12	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49098	6.7e-56	74.63	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O04354	2.7e-52	73.08	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]
P49100	7.7e-52	74.22	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of MC07g0523 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138846.1	1.22e-132	100.00	cytochrome b5-like [Momordica charantia]	[more]
XP_022959033.1	4.10e-86	91.79	cytochrome b5-like [Cucurbita moschata]	[more]
XP_023006481.1	5.82e-86	91.79	cytochrome b5-like [Cucurbita maxima]	[more]
KAG6574866.1	1.17e-85	91.04	Cytochrome b5 isoform B, partial [Cucurbita argyrosperma subsp. sororia] >KAG701...	[more]
XP_004138957.1	3.36e-85	89.55	cytochrome b5 [Cucumis sativus] >KGN61465.1 hypothetical protein Csa_005989 [Cuc...	[more]

BLAST of MC07g0523 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAX1	5.90e-133	100.00	cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111009917 PE=3 SV=1	[more]
A0A6J1H4S5	1.98e-86	91.79	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111460140 PE=3 SV=1	[more]
A0A6J1KXV9	2.82e-86	91.79	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111499200 PE=3 SV=1	[more]
A0A0A0LHS9	1.63e-85	89.55	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A5D3CSF5	9.41e-85	88.81	Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold472G0...	[more]

BLAST of MC07g0523 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	1.3e-59	78.95	cytochrome B5 isoform B	[more]
AT5G53560.1	2.5e-50	67.42	cytochrome B5 isoform E	[more]
AT5G48810.1	1.1e-48	62.96	cytochrome B5 isoform D	[more]
AT2G46650.1	1.2e-28	46.03	cytochrome B5 isoform C	[more]
AT1G26340.1	8.0e-28	42.52	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 79..89 score: 61.02 coord: 104..111 score: 48.11 coord: 89..103 score: 53.54 coord: 117..129 score: 35.08
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 57..130 e-value: 1.3E-34 score: 131.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 58..129 e-value: 8.5E-27 score: 93.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 54..130 score: 29.38707
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 49..134 e-value: 5.9E-35 score: 121.1
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 54..135
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 50..181
None	No IPR available	PANTHER	PTHR19359:SF103	CYTOCHROME B5-LIKE	coord: 50..181
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 85..92

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0523.1	MC07g0523.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043447	alkane biosynthetic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding