MC07g0521 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0521
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPectinesterase
LocationMC07: 13225973 .. 13230692 (-)
RNA-Seq ExpressionMC07g0521
SyntenyMC07g0521
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGATTTTTGTTCGAGGGATTTGTGCATTGAGCATTGGAATAGCGATAATATTTGGGAGAGGAACAGAAGCTCAGTCTTATCGATCTGTGGGGGAGAAGAAGGTGGCATGGCGGACTGTGGTGGTGGACCAGAGCGGCCATGGAAATTTCTCCACTATTCAAGCCGCCATTGATGATGTTCCTTCAAACAATAGGTTTTGGGTCTCCATTTTAGTCAAGGCTGGAGTTTACAGGTTCTTTTTTCTTCCCTTTTTTGATACCTCACTTTCGTATTTTTTTTTTACGTTCTAGCTGTTTTTGTGTGAATAGTCGTTGAGTTTTGAATATGAGGCTCGGTTTGATAATCATTTTGATGTTGGTTTTCTGCTTATGAAAATTGAAGGGGTAGACACTACTTTCTCTGATCACTGGGCGGAGTTTAAAATTTTAACGGTCTAACTGTTTTTATATGAATAGTTAAGTTTTGAATATAAGACTCCATTTGATAATATTTTGATTTTGGTGTTCTGTTTATGAAAATTTAAAATTTTACATTGTTCTTGTATGAATAGTTAAATTTTGAATATGAGACTCCGTTCGGTAATATTTTGATTTTGGTTTTGAAAATTAAGGTGGTAGGCACTAATTATTTCATCTATTTGCCTCTGCCTTTTGGAATTGGTTTCAAAATCTAACCAAAATTTTGAAAATAGTTTTCTTAAAATTTATTTTTGGGAAAGACCCAAAACATATTAAAGAAATTGTGACCAAACAGAGTAGAAAATTGATTTGGATGATTTTATTCAATTTAGGTATCAATTTTTGCCGAAAATGGAGCTAAATTGATTTATTTACTCCCTATTTTCTCCCCTTAATCATATTTAGCCTCGCCATTTCATTTATAATTACTCTCTTGAAAATTCAAAACTTTTGTACTTCAAAAGATTGAACTTAAAGTAATTAAATACCTAGTAATTTACAAATTACCCCATAAATAATAAAACATTTTTACAGTTAATATTCTACAAGTTTCATTGTCAACCAATTCTAGTAGGGTCAGGTGGTTGGTCCGTGATTAGTCGATGTACATACAAGCTAATTCAGACACACGGATAAAATAAAGGTATTATTGAAAATCAAATGGATAATATTATTGACCATGTGTTTTTTTCCTTGGCATTTGTGATATATTTTAGAGAGAAGGTGACAATCCCATATGATAAGCCATATATAATTCTGAAGGGACAAGGGAAGAGAAGAACAAGAGTTGTTTGGGATGACCATCTCAATATTGCTCAAAGCCCTACTTTCACTTCTTCTGCTGATAATATTGTTGTCAAATGTATAAGTTTTGAGGTATGTCAAAAATGCATCAAAACCATATCCCTAAACAAATCTAGGGTTTTTCTAGCTCTCGATCGGTCCCCTTTGATAACCCATTTTGATTTTTGTTGTTCTTTGCACGTTTCATTTTTCAAAAAGCAAATTGAAGCTGTTGATAAACTCGTTCGAGTTTTAACCTTTTAAGTTTTTCTTTTTCGATGAGTTTGTGTTAGACTTGTTTACTAACATTGTTGTTTCATATCTAATCTATTTTCAAATTTGCTTTTTAAAATCATAACCAAAATTTAAAACTAAAAGTTTAATAACTGAGTGGTTTTCCAAAAACCCATTTATTGATTTTGAAAATTGGACTAAAATTTCTAAGGTGTAGAAAAATATGTATTTTCTTTAAGCTAAAACTAAAAAAAAAAAAAAAAGAACCCCACTTATCATTTGTTCTTAGGAAATTAATCTTAAAAATTGTGAACAGCATTAAATTGATGTTATGAAAGTAAGATTAAAGCAATTTTTTAATAACAAAAAGTAATTTTCGAGTTGTTCTCGATCATATTATTTCGAGGGTAAATTATTTTATTTTATTTTATTCAACTTTCGTTATAGTTTGAAAATAATTTTCCAAACAAACCCTTCTTAGTTTCTCTTCTTTTTTATTTATTCTTAGGAATTAAGTTATTTTCTTCCGTTTTATTTTATTATTTTATTTTATTTTTGGGTTTCAGAATTCATATAATGATCCATGGAACAATGGAAACCCAAGGGTTCCAGCAGTAGCAGCGATGGTGACAGGCGACAAGTCGTCTTTCTATAAATGTGGTTTCTATGGCTTACAAGACACCTTATGGGATGCCCTTGGACGACACTACTTCCATCGTTGCACTATCCAAGGAGCTGTCGACTTTATTTTTGGAACTGCCCAATCCATTTATGAGGTACATATATGATCAATATATAATTTCACATTTCTATTATTGCAATTCTATCAAACTCAAATGATCCGATAGAGACTAGATTTTATCTTGTTTTCCTTTGAATTAGTTACGAATAGGGCAACAACGTCCCCAGAAAAAGAAGTTGGCTTATTAAATTAATACAATAAGAAAGAGCTCCTCGATCAAAGGAAGCACGAGTTTTTGGCATCTAGTATCATTTTTTATTAGTAGTGTCATGATCAGGGCTGTAGTACTGATTATGCATAGGACTGCTACGGTGAAAAGACAAAATGTATTCCAATAAAATGCTATTGATTTCAGGAGGACGTCTTTGTGAAAAAGATATATATCACTACCTTTTAGGTCCTTGATTCGTTTTACCGTAGAAGCTAACGATAGATAATAACTCCCCTCAAAATACAAACTTATTACAACTTCTCATTCATTTGAGTAACATTATTTCAGTAAATAGAAGAATCGTGAATAGGGAACTATACTAGGAACCCCACCTAATTTTATTGTATAATTTTTGGGCTCCATGACGATTGGATCATGCAAACGTCAGCTATTGGCCGATCTAAATAAATTTTGTTGGTTTTGGTCAACTCAGCTCGATTTTTTATGAGTTAAATTCAAACTAAACCGAACTAACTCATTTTGTGTGACGTTTGGTTGTAGGGATGTACAATATCAGTAGTCGGAGAAGCGCTCCCACCAAGAATCACAGGATTTATAACAGCACAAGGAAGAACAGACCCAAATGATGGAAATGGGTTTGTTTTCAAAGATTGTAAAGTGGTTGGGAGTGGCTCAACCTTCTTGGGGAGGCCATGGAGAGGCTACTCAAGAGTCATCTTCTACAACTCTAACTTCTCCAACATCATAAACCCTAATGGTTGGGATCCTTGGCGTTTTGCTGGCCAAGAGTAAGATATTTCATCCATCTATCTAATTTTTTTTTTGTCATTTTTCATTTGTCTATTTCTATATATATTTCTTTCATGTTTGGTTTAAATACTATTTTAGTTCGTGTATTTTAGATTTTGGTTCTTCTACTTTCAAAATGTTCCTATACTTTCAACATTTATTTATTTTAGTTCGTATTTCTAAAGTGTTCATTTTTGTTCTGTAGGCTTTTAAAAGCAATCAATTTGGTCACTTCATTTTTATTTCATTTTTCAATTATAATTTTAGTATATTGACATTTCACTCACAAAATTTTCTTGTGTGTAAAATCTGTTTGGTCACTCTTTAAAAGTACAGAGATCAAAATGAATCTTTTAAAAATATTAAAGACTAAAATGAACAAAACTTGAAACTATAGGATCAGTGGACATTTTAAAAGTAAGCAGAGACCCAAATGAACCAAAGCTAAAAGAACGATGGACTAAAATAATATTTAAATCTTATTATTTCTTTACTTCTTTTCTTCATTTCACCATTAAATTTGCTTGTTTTTTTAATATATATTTCCATTTGAAAATGGGGAAACTTGAAAAGATTGATTGGTACTTGGGGGTGGGTTTTGGAGTTGGTAAGCACTTAGGTCTAAAGAAAAAAAGAGGGTTTTGTTTCTTTTGTTGGCTTTTGGTGTTGGATTCAAAATTTCAATCATGGATTTTGGATTTATTCTATTGGTTTGATTTATGATTTCTTCTTTTTGTTTTTCTAATTTAAAAGATTATGTTTGTTTGCTCGGAATTTTTTTTTCATATTTTTAAAAACATTTGAAATTCGTAGTCAATTTTTGAAAATTAAAACAAGTTTTTTCAAAACTACTTTTTTTTAGTTTTCAAAATTTAACTTAAAATTTGAACAGATTTAGATGAAGGAAGTGTTTATCACCCTGATATTAAAACATAAATATAAAATAAAATGGTTATGAAAAAAATAACTTTTGAAAAATAAGTTTTTGATTTTTTAAAGGCAGTGATAATTCAAACAAAATATAAAGCTATAATCATTTTATTTTCTATAAGTTTGTAAACTAGTTTCCACCAAGCTTTTTCTTTTTTCTACTTTTTAAAAACGTTTTAAAAAATCTAAGCCAAAATTTTTAAAATTATTTTTATTTTTTTTAATTTAGTTAAGAATTAAATGTCTTCTTAAGAAAAATAGGAATTATTTTATTCTGTTACTCGTTTAAGAAAAAAAATTACCAATTGTGTAGTTTTATTTATTTATTATTATTATTATTTTTTTGGTGTGGAGTAGAGAATTATTTTAAAATGTTACTACATTTGAAAAATAAATAAATTATAAAACACAAATTATTTAGCTCCTCCTGATGAATAATTTCTTGAAAATCCAGGGGCCAATTGACCTACGCAGAATATGGCTGCTACGGACCAGGTTCCGACATGTCGCACCGCGTGAAATGGGAAAAGAAGCTCAGTTCGGAGGAAATACGACAACTAACCAGCATGAGTTTTGTCGACACTGAAGGCTGGCTCCAAAACCAACCT

mRNA sequence

ATGTGGATTTTTGTTCGAGGGATTTGTGCATTGAGCATTGGAATAGCGATAATATTTGGGAGAGGAACAGAAGCTCAGTCTTATCGATCTGTGGGGGAGAAGAAGGTGGCATGGCGGACTGTGGTGGTGGACCAGAGCGGCCATGGAAATTTCTCCACTATTCAAGCCGCCATTGATGATGTTCCTTCAAACAATAGGTTTTGGGTCTCCATTTTAGTCAAGGCTGGAGTTTACAGAGAGAAGGTGACAATCCCATATGATAAGCCATATATAATTCTGAAGGGACAAGGGAAGAGAAGAACAAGAGTTGTTTGGGATGACCATCTCAATATTGCTCAAAGCCCTACTTTCACTTCTTCTGCTGATAATATTGTTGTCAAATGTATAAGTTTTGAGAATTCATATAATGATCCATGGAACAATGGAAACCCAAGGGTTCCAGCAGTAGCAGCGATGGTGACAGGCGACAAGTCGTCTTTCTATAAATGTGGTTTCTATGGCTTACAAGACACCTTATGGGATGCCCTTGGACGACACTACTTCCATCGTTGCACTATCCAAGGAGCTGTCGACTTTATTTTTGGAACTGCCCAATCCATTTATGAGGGATGTACAATATCAGTAGTCGGAGAAGCGCTCCCACCAAGAATCACAGGATTTATAACAGCACAAGGAAGAACAGACCCAAATGATGGAAATGGGTTTGTTTTCAAAGATTGTAAAGTGGTTGGGAGTGGCTCAACCTTCTTGGGGAGGCCATGGAGAGGCTACTCAAGAGTCATCTTCTACAACTCTAACTTCTCCAACATCATAAACCCTAATGGTTGGGATCCTTGGCGTTTTGCTGGCCAAGAGGGCCAATTGACCTACGCAGAATATGGCTGCTACGGACCAGGTTCCGACATGTCGCACCGCGTGAAATGGGAAAAGAAGCTCAGTTCGGAGGAAATACGACAACTAACCAGCATGAGTTTTGTCGACACTGAAGGCTGGCTCCAAAACCAACCT

Coding sequence (CDS)

ATGTGGATTTTTGTTCGAGGGATTTGTGCATTGAGCATTGGAATAGCGATAATATTTGGGAGAGGAACAGAAGCTCAGTCTTATCGATCTGTGGGGGAGAAGAAGGTGGCATGGCGGACTGTGGTGGTGGACCAGAGCGGCCATGGAAATTTCTCCACTATTCAAGCCGCCATTGATGATGTTCCTTCAAACAATAGGTTTTGGGTCTCCATTTTAGTCAAGGCTGGAGTTTACAGAGAGAAGGTGACAATCCCATATGATAAGCCATATATAATTCTGAAGGGACAAGGGAAGAGAAGAACAAGAGTTGTTTGGGATGACCATCTCAATATTGCTCAAAGCCCTACTTTCACTTCTTCTGCTGATAATATTGTTGTCAAATGTATAAGTTTTGAGAATTCATATAATGATCCATGGAACAATGGAAACCCAAGGGTTCCAGCAGTAGCAGCGATGGTGACAGGCGACAAGTCGTCTTTCTATAAATGTGGTTTCTATGGCTTACAAGACACCTTATGGGATGCCCTTGGACGACACTACTTCCATCGTTGCACTATCCAAGGAGCTGTCGACTTTATTTTTGGAACTGCCCAATCCATTTATGAGGGATGTACAATATCAGTAGTCGGAGAAGCGCTCCCACCAAGAATCACAGGATTTATAACAGCACAAGGAAGAACAGACCCAAATGATGGAAATGGGTTTGTTTTCAAAGATTGTAAAGTGGTTGGGAGTGGCTCAACCTTCTTGGGGAGGCCATGGAGAGGCTACTCAAGAGTCATCTTCTACAACTCTAACTTCTCCAACATCATAAACCCTAATGGTTGGGATCCTTGGCGTTTTGCTGGCCAAGAGGGCCAATTGACCTACGCAGAATATGGCTGCTACGGACCAGGTTCCGACATGTCGCACCGCGTGAAATGGGAAAAGAAGCTCAGTTCGGAGGAAATACGACAACTAACCAGCATGAGTTTTGTCGACACTGAAGGCTGGCTCCAAAACCAACCT

Protein sequence

MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP
Homology
BLAST of MC07g0521 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.2e-116
Identity = 189/299 (63.21%), Postives = 229/299 (76.59%), Query Frame = 0

Query: 41  VVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRR 100
           V VDQSGHGNF+TIQ AID VP NNR W  I VKAG+YREK+ IPY+KP+I+L G GKR 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 101 TRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWN---NGNPRVPAVAAMVTGDK 160
           TRV WDDH ++AQSPTF++ ADN VVK I+F NSYN P     N NPR PAVAA++ GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 161 SSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRI 220
           S+FY  GF G+QDTLWD  GRHYFHRCTIQGAVDFIFGT QSIY+ C I V+G  L P +
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGL 213

Query: 221 TGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWD 280
            G+ITAQGRT+P D NGF+F +C V G+G  FLGRPWRGYSRVIFYNSN ++++ P GWD
Sbjct: 214 AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGWD 273

Query: 281 PWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            W F G E QL +AE+GC+G G+++  RVKW KKLS   I+ L  +SF++  GW+++ P
Sbjct: 274 AWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 332

BLAST of MC07g0521 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 5.5e-112
Identity = 186/299 (62.21%), Postives = 227/299 (75.92%), Query Frame = 0

Query: 41  VVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRR 100
           V+VDQSGHGNF+TIQ AID VP NN  W  I VKAG+YREK+TIP  KP+I++ G GKR 
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 101 TRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWN---NGNPRVPAVAAMVTGDK 160
           TRV WDDH ++AQSPTF + ADN VVK I+F NSYN P N   N NPRVPAVAA + GDK
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDK 148

Query: 161 SSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRI 220
           S+FY  GF G+QDTLWD+ GRHYFHRCTIQGAVDFI G+ QSIY+ C I V+G  L P +
Sbjct: 149 SAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGPGV 208

Query: 221 TGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWD 280
           TG+ITAQGRT+ ND NGFVF +C V G G  +LGR WR YSRVIFYNSN +++++P GW 
Sbjct: 209 TGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPLGWW 268

Query: 281 PWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            W + G E QLTYAE+GC+G GS+ S R KW KKLS+  ++ L  +SF++  GW+++ P
Sbjct: 269 EWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVEDLP 327

BLAST of MC07g0521 vs. ExPASy Swiss-Prot
Match: O64479 (Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.7e-84
Identity = 148/299 (49.50%), Postives = 196/299 (65.55%), Query Frame = 0

Query: 39  RTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGK 98
           +T++V+ +    F T+Q+AID +P  N+ W+ IL+  G+Y EKVTIP  K YI ++G G 
Sbjct: 39  KTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGGI 98

Query: 99  RRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKS 158
            +T + + DH     S TFTS   NI++  I+F+N YN   ++ +P  PAVAAM+ GDK 
Sbjct: 99  EKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SSSSPTKPAVAAMMLGDKY 158

Query: 159 SFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISV-VGEALPPRI 218
           +     F G QDTL+D  GRHY+ RC I G +DFIFG AQSI+EGCT+ + VG   P  +
Sbjct: 159 AIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPPNEV 218

Query: 219 TGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWD 278
            G ITAQGR  P D  GFVFKDC V+GSG   LGR W+ YSRVIFY S FS+ I P GWD
Sbjct: 219 YGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPIGWD 278

Query: 279 PWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            W+  GQEG +T+ E+GC G G+D S RV W  K S +++ Q T+++F+D EGWL   P
Sbjct: 279 AWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWLSRLP 336

BLAST of MC07g0521 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 294.7 bits (753), Expect = 1.4e-78
Identity = 143/308 (46.43%), Postives = 194/308 (62.99%), Query Frame = 0

Query: 30  SVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKP 89
           S   +++A+ T+ VD +G GNF+T+Q+AID +   N  W+ +  + G+YREKVTIP +K 
Sbjct: 25  SAAAEQIAY-TITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKG 84

Query: 90  YIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGNPRVPAV 149
           +I L+G+G  +T + +DDH     S TFT+ AD+IV+  I+F+N+YN   NN    VPAV
Sbjct: 85  FIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPNNKREIVPAV 144

Query: 150 AAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISV- 209
           AA + GD+       F GLQDTL+D  GRHY+ RC I G +DFIFG  QS+++ CT+++ 
Sbjct: 145 AARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMT 204

Query: 210 VGEALPPRITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFS 269
           +G   P    G ITA  R  P+D  GFVF DC V G G T LGR W   +RVIF  S  S
Sbjct: 205 LGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLS 264

Query: 270 NIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDT 329
           +++ P GWD WR  G E  LT+ E GC G G+D S RV W KKLS  E+    S+SF+D 
Sbjct: 265 DVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQ 324

Query: 330 EGWLQNQP 337
           +GW+   P
Sbjct: 325 DGWISRFP 331

BLAST of MC07g0521 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.7e-69
Identity = 135/296 (45.61%), Postives = 173/296 (58.45%), Query Frame = 0

Query: 43  VDQSGHGNFSTIQAAIDDVPS--NNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRR 102
           VDQSG G+FS IQ AI+ +P   NN     I VK G+YREKV IP +KPYI L G     
Sbjct: 53  VDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASN 112

Query: 103 TRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSF 162
           T ++W D  +I +SPT T  A + V + ++ +N +            AVA  V  DK++F
Sbjct: 113 TFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGR-------AVALRVAADKAAF 172

Query: 163 YKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGF 222
           Y C     QDTL D  G HYF  C I+GA DFI G+A S+YE C +     +L P   G 
Sbjct: 173 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL----HSLSPN-NGS 232

Query: 223 ITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWR 282
           ITAQ RT   + +GF F  CK+ GSGSTFLGRPW  YSRV+F  S FSN++ P GW+ W 
Sbjct: 233 ITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWG 292

Query: 283 FAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            + +E  + Y EY CYGPG+D   RV+W K+LS EE     S  F+  + WL+  P
Sbjct: 293 DSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAP 336

BLAST of MC07g0521 vs. NCBI nr
Match: XP_022138633.1 (probable pectinesterase 29 [Momordica charantia])

HSP 1 Score: 712 bits (1838), Expect = 1.60e-259
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60
           MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD
Sbjct: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60

Query: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120
           VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS
Sbjct: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120

Query: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180
           ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY
Sbjct: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180

Query: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDC 240
           FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDC
Sbjct: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDC 240

Query: 241 KVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGS 300
           KVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGS
Sbjct: 241 KVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGS 300

Query: 301 DMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336
           DMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP
Sbjct: 301 DMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336

BLAST of MC07g0521 vs. NCBI nr
Match: XP_038875009.1 (probable pectinesterase 29 [Benincasa hispida])

HSP 1 Score: 572 bits (1474), Expect = 4.83e-204
Identity = 268/337 (79.53%), Postives = 295/337 (87.54%), Query Frame = 0

Query: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60
           MWI +RGI    I + IIF RG E QSYRSVG KK+ W+T++VDQ+GHGNFSTIQAAID 
Sbjct: 1   MWI-IRGIFVGLILMMIIFVRGNEGQSYRSVGMKKLTWKTLIVDQNGHGNFSTIQAAIDS 60

Query: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120
           +PSNNRFWVSI VK GVYREKV IPYDKPYIILKGQ KR T+VVWDDHL +AQSPTFTSS
Sbjct: 61  IPSNNRFWVSIHVKPGVYREKVKIPYDKPYIILKGQSKRTTKVVWDDHLTVAQSPTFTSS 120

Query: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180
           ADNIVVK I+F NSYN PWNNGNPRVPAVAAM+TGDKSSFYKCGFYG+QDTLWD  GRHY
Sbjct: 121 ADNIVVKSITFVNSYNYPWNNGNPRVPAVAAMITGDKSSFYKCGFYGVQDTLWDDQGRHY 180

Query: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPR-ITGFITAQGRTDPNDGNGFVFKD 240
           +HRCTIQGAVDFIFG AQSI++GC+ISVVGEAL P   T FITAQGRT+PND NGFVFK+
Sbjct: 181 YHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTNPNDANGFVFKE 240

Query: 241 CKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPG 300
           C V GSGS FLGRPWRGYSRVIFYNSNFSNIINPNGWDPW F GQE QLTYAE GCYGPG
Sbjct: 241 CNVFGSGSAFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWHFVGQENQLTYAENGCYGPG 300

Query: 301 SDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336
           +DMSHRV+WEKKLS  EIRQLTS++F++ E W+Q+QP
Sbjct: 301 ADMSHRVEWEKKLSLNEIRQLTSLNFINGESWIQDQP 336

BLAST of MC07g0521 vs. NCBI nr
Match: XP_023005997.1 (probable pectinesterase 29 [Cucurbita maxima])

HSP 1 Score: 559 bits (1440), Expect = 4.87e-199
Identity = 258/325 (79.38%), Postives = 282/325 (86.77%), Query Frame = 0

Query: 13  IGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSIL 72
           + + IIF RG E + YRSVG KK+AWRTVVVDQSGHGNFSTIQAAID VPSNN FWVSI 
Sbjct: 1   MAMMIIFVRGNENERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWVSIH 60

Query: 73  VKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFE 132
           +  G+YREKV IPYDKPYIILKGQ ++ T+VVWDDH  +AQSPTFTSSADN+VVK I+F 
Sbjct: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWDDHFTLAQSPTFTSSADNVVVKSITFV 120

Query: 133 NSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDF 192
           NSYN PW NGN R PAVAAM+TGDK+SFYKCGFYG+QDTLWD  GRHY+HRC IQGAVDF
Sbjct: 121 NSYNFPWTNGNRRAPAVAAMITGDKASFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180

Query: 193 IFGTAQSIYEGCTISVVGEA-LPPRITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLG 252
           IFG+AQSI++GC ISVVGEA LP   T FITAQGRTDPND NGFVFKDCKV GSGS FLG
Sbjct: 181 IFGSAQSIFQGCLISVVGEAFLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240

Query: 253 RPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKK 312
           RPWRGYSRVIFYNS+FSNIINPNGWDPW F G E QLTYAEYGCYGPGSDMSHRVKWEKK
Sbjct: 241 RPWRGYSRVIFYNSSFSNIINPNGWDPWHFVGHEDQLTYAEYGCYGPGSDMSHRVKWEKK 300

Query: 313 LSSEEIRQLTSMSFVDTEGWLQNQP 336
           L   EI+QLT MSF+DTEGW+Q+QP
Sbjct: 301 LDLNEIQQLTDMSFIDTEGWMQDQP 325

BLAST of MC07g0521 vs. NCBI nr
Match: XP_022958865.1 (probable pectinesterase 29 [Cucurbita moschata])

HSP 1 Score: 555 bits (1430), Expect = 1.62e-197
Identity = 254/325 (78.15%), Postives = 283/325 (87.08%), Query Frame = 0

Query: 13  IGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSIL 72
           +G+ IIF RG E + YRSVG KK+AWRTVVVDQSGHGNFSTIQAAID VPSNN FW+SI 
Sbjct: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60

Query: 73  VKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFE 132
           +  G+YREKV IPYDKPYIILKGQ ++ T+VVW+DH  +AQSPTFTSSADN+VVK I+F 
Sbjct: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120

Query: 133 NSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDF 192
           NSYN PW NGN R PAVAAM+TGDK+ FYKCGFYG+QDTLWD  GRHY+HRC IQGAVDF
Sbjct: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180

Query: 193 IFGTAQSIYEGCTISVVGEALPPR-ITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLG 252
           IFG+AQSI++GC+ISVVGEAL P   T FITAQGRTDPND NGFVFKDCKV GSGS FLG
Sbjct: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240

Query: 253 RPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKK 312
           RPWRGYSRVIFYNS+FSN+INP+GWDPW F G E QLTYAEYGCYGPGSDMSHRVKWEKK
Sbjct: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHEDQLTYAEYGCYGPGSDMSHRVKWEKK 300

Query: 313 LSSEEIRQLTSMSFVDTEGWLQNQP 336
           L   EI+QLT MSF+DTEGW+Q+QP
Sbjct: 301 LDLNEIQQLTDMSFIDTEGWMQDQP 325

BLAST of MC07g0521 vs. NCBI nr
Match: XP_008457276.1 (PREDICTED: probable pectinesterase 29 [Cucumis melo])

HSP 1 Score: 555 bits (1431), Expect = 1.65e-197
Identity = 257/337 (76.26%), Postives = 288/337 (85.46%), Query Frame = 0

Query: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60
           MW  +RGI  L +   IIF RG E +SYR +G KK AW+T++VD++GHGNFSTIQAAID 
Sbjct: 1   MWTIIRGIFVLMM---IIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDS 60

Query: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120
           VPS+NRFWVSI ++ G+YREKV IPYDKPYIILKG  KR T+VVWDDHL +AQSPTFTSS
Sbjct: 61  VPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSS 120

Query: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180
           ADNIVVK ISF NSYN PW NGNPRVPAVAAM+TGDKSSFY+CGFYG+QDTLWD  GRHY
Sbjct: 121 ADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHY 180

Query: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPR-ITGFITAQGRTDPNDGNGFVFKD 240
           +HRCTIQGAVDFIFG AQSI++GC+ISVVGEAL P   T FITAQGRTDPND NGFVFK+
Sbjct: 181 YHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKE 240

Query: 241 CKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPG 300
           C V GSGS +LGRPWR YSRVIF+NSNFSNIINPNGWDPW F G E QLTYAE  CYGPG
Sbjct: 241 CNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPG 300

Query: 301 SDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336
           SD+S RV WEKKLS ++IRQLTSMSF+D EGW+Q+QP
Sbjct: 301 SDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 334

BLAST of MC07g0521 vs. ExPASy TrEMBL
Match: A0A6J1CDJ5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009749 PE=4 SV=1)

HSP 1 Score: 712 bits (1838), Expect = 7.77e-260
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60
           MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD
Sbjct: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60

Query: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120
           VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS
Sbjct: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120

Query: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180
           ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY
Sbjct: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180

Query: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDC 240
           FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDC
Sbjct: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDC 240

Query: 241 KVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGS 300
           KVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGS
Sbjct: 241 KVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGS 300

Query: 301 DMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336
           DMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP
Sbjct: 301 DMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336

BLAST of MC07g0521 vs. ExPASy TrEMBL
Match: A0A6J1KUQ1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 2.36e-199
Identity = 258/325 (79.38%), Postives = 282/325 (86.77%), Query Frame = 0

Query: 13  IGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSIL 72
           + + IIF RG E + YRSVG KK+AWRTVVVDQSGHGNFSTIQAAID VPSNN FWVSI 
Sbjct: 1   MAMMIIFVRGNENERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWVSIH 60

Query: 73  VKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFE 132
           +  G+YREKV IPYDKPYIILKGQ ++ T+VVWDDH  +AQSPTFTSSADN+VVK I+F 
Sbjct: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWDDHFTLAQSPTFTSSADNVVVKSITFV 120

Query: 133 NSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDF 192
           NSYN PW NGN R PAVAAM+TGDK+SFYKCGFYG+QDTLWD  GRHY+HRC IQGAVDF
Sbjct: 121 NSYNFPWTNGNRRAPAVAAMITGDKASFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180

Query: 193 IFGTAQSIYEGCTISVVGEA-LPPRITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLG 252
           IFG+AQSI++GC ISVVGEA LP   T FITAQGRTDPND NGFVFKDCKV GSGS FLG
Sbjct: 181 IFGSAQSIFQGCLISVVGEAFLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240

Query: 253 RPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKK 312
           RPWRGYSRVIFYNS+FSNIINPNGWDPW F G E QLTYAEYGCYGPGSDMSHRVKWEKK
Sbjct: 241 RPWRGYSRVIFYNSSFSNIINPNGWDPWHFVGHEDQLTYAEYGCYGPGSDMSHRVKWEKK 300

Query: 313 LSSEEIRQLTSMSFVDTEGWLQNQP 336
           L   EI+QLT MSF+DTEGW+Q+QP
Sbjct: 301 LDLNEIQQLTDMSFIDTEGWMQDQP 325

BLAST of MC07g0521 vs. ExPASy TrEMBL
Match: A0A6J1H4P1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1)

HSP 1 Score: 555 bits (1430), Expect = 7.86e-198
Identity = 254/325 (78.15%), Postives = 283/325 (87.08%), Query Frame = 0

Query: 13  IGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSIL 72
           +G+ IIF RG E + YRSVG KK+AWRTVVVDQSGHGNFSTIQAAID VPSNN FW+SI 
Sbjct: 1   MGMMIIFVRGNEDERYRSVGGKKLAWRTVVVDQSGHGNFSTIQAAIDSVPSNNMFWISIH 60

Query: 73  VKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFE 132
           +  G+YREKV IPYDKPYIILKGQ ++ T+VVW+DH  +AQSPTFTSSADN+VVK I+F 
Sbjct: 61  INPGIYREKVKIPYDKPYIILKGQRRKTTKVVWNDHFTLAQSPTFTSSADNVVVKSITFV 120

Query: 133 NSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDF 192
           NSYN PW NGN R PAVAAM+TGDK+ FYKCGFYG+QDTLWD  GRHY+HRC IQGAVDF
Sbjct: 121 NSYNFPWTNGNRRAPAVAAMITGDKALFYKCGFYGVQDTLWDDQGRHYYHRCVIQGAVDF 180

Query: 193 IFGTAQSIYEGCTISVVGEALPPR-ITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLG 252
           IFG+AQSI++GC+ISVVGEAL P   T FITAQGRTDPND NGFVFKDCKV GSGS FLG
Sbjct: 181 IFGSAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKDCKVFGSGSAFLG 240

Query: 253 RPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKK 312
           RPWRGYSRVIFYNS+FSN+INP+GWDPW F G E QLTYAEYGCYGPGSDMSHRVKWEKK
Sbjct: 241 RPWRGYSRVIFYNSSFSNVINPSGWDPWHFVGHEDQLTYAEYGCYGPGSDMSHRVKWEKK 300

Query: 313 LSSEEIRQLTSMSFVDTEGWLQNQP 336
           L   EI+QLT MSF+DTEGW+Q+QP
Sbjct: 301 LDLNEIQQLTDMSFIDTEGWMQDQP 325

BLAST of MC07g0521 vs. ExPASy TrEMBL
Match: A0A1S3C544 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1)

HSP 1 Score: 555 bits (1431), Expect = 7.99e-198
Identity = 257/337 (76.26%), Postives = 288/337 (85.46%), Query Frame = 0

Query: 1   MWIFVRGICALSIGIAIIFGRGTEAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDD 60
           MW  +RGI  L +   IIF RG E +SYR +G KK AW+T++VD++GHGNFSTIQAAID 
Sbjct: 1   MWTIIRGIFVLMM---IIFVRGNEGESYRRIGRKKFAWKTLIVDKNGHGNFSTIQAAIDS 60

Query: 61  VPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSS 120
           VPS+NRFWVSI ++ G+YREKV IPYDKPYIILKG  KR T+VVWDDHL +AQSPTFTSS
Sbjct: 61  VPSDNRFWVSIHIRPGLYREKVKIPYDKPYIILKGHRKRNTKVVWDDHLTVAQSPTFTSS 120

Query: 121 ADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHY 180
           ADNIVVK ISF NSYN PW NGNPRVPAVAAM+TGDKSSFY+CGFYG+QDTLWD  GRHY
Sbjct: 121 ADNIVVKSISFVNSYNYPWKNGNPRVPAVAAMITGDKSSFYRCGFYGVQDTLWDNQGRHY 180

Query: 181 FHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPR-ITGFITAQGRTDPNDGNGFVFKD 240
           +HRCTIQGAVDFIFG AQSI++GC+ISVVGEAL P   T FITAQGRTDPND NGFVFK+
Sbjct: 181 YHRCTIQGAVDFIFGAAQSIFQGCSISVVGEALLPLGSTSFITAQGRTDPNDANGFVFKE 240

Query: 241 CKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPG 300
           C V GSGS +LGRPWR YSRVIF+NSNFSNIINPNGWDPW F G E QLTYAE  CYGPG
Sbjct: 241 CNVFGSGSAYLGRPWRAYSRVIFHNSNFSNIINPNGWDPWHFVGYENQLTYAENDCYGPG 300

Query: 301 SDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 336
           SD+S RV WEKKLS ++IRQLTSMSF+D EGW+Q+QP
Sbjct: 301 SDISRRVSWEKKLSPKDIRQLTSMSFIDVEGWIQDQP 334

BLAST of MC07g0521 vs. ExPASy TrEMBL
Match: W9SM62 (Pectinesterase OS=Morus notabilis OX=981085 GN=L484_021593 PE=4 SV=1)

HSP 1 Score: 482 bits (1241), Expect = 3.91e-169
Identity = 220/313 (70.29%), Postives = 256/313 (81.79%), Query Frame = 0

Query: 24  EAQSYRSVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVT 83
             Q YR VG KK A+ T+ VD SG GNFSTIQ+AI+ VPSNN++WV I +KAG+YREK+ 
Sbjct: 9   NGQWYRKVGYKKFAYYTITVDPSGQGNFSTIQSAINSVPSNNKYWVCIRIKAGIYREKIK 68

Query: 84  IPYDKPYIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGN 143
           IPYDKPYIILKG+G+R+T+VVWDDH  +AQSPTFTS ADNIV KC+SF NSYN P N+  
Sbjct: 69  IPYDKPYIILKGEGRRKTQVVWDDHDTLAQSPTFTSLADNIVAKCMSFTNSYNRPPND-K 128

Query: 144 PRVPAVAAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEG 203
           PR PA AAM+TGDK+ F++CGF+GLQDTLWD  GRHY+  CTIQGAVDFIFG+ QSI+E 
Sbjct: 129 PRAPAAAAMITGDKTKFFRCGFFGLQDTLWDDQGRHYYKLCTIQGAVDFIFGSGQSIFER 188

Query: 204 CTISVVGEALPPRITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFY 263
           C+ISVVG AL P   GFITAQGRT+PND NGFVFKDC V GSGS FLGRPWRGY+RV FY
Sbjct: 189 CSISVVGGALEPGFQGFITAQGRTNPNDANGFVFKDCHVFGSGSAFLGRPWRGYARVFFY 248

Query: 264 NSNFSNIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSM 323
           N+N S++I P GW+ W FAGQE QLTYAE GC+GPGSD S RV WEKKL+ E ++QLTS 
Sbjct: 249 NTNISDVIVPQGWNAWNFAGQEYQLTYAEEGCFGPGSDSSRRVSWEKKLNEETVKQLTSS 308

Query: 324 SFVDTEGWLQNQP 336
           SF+D EGWL NQP
Sbjct: 309 SFIDDEGWLNNQP 320

BLAST of MC07g0521 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 418.7 bits (1075), Expect = 4.4e-117
Identity = 189/299 (63.21%), Postives = 229/299 (76.59%), Query Frame = 0

Query: 41  VVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRR 100
           V VDQSGHGNF+TIQ AID VP NNR W  I VKAG+YREK+ IPY+KP+I+L G GKR 
Sbjct: 34  VFVDQSGHGNFTTIQKAIDSVPINNRHWFFINVKAGLYREKIKIPYEKPFIVLVGAGKRL 93

Query: 101 TRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWN---NGNPRVPAVAAMVTGDK 160
           TRV WDDH ++AQSPTF++ ADN VVK I+F NSYN P     N NPR PAVAA++ GDK
Sbjct: 94  TRVEWDDHYSVAQSPTFSTLADNTVVKSITFANSYNFPSKGKMNKNPRTPAVAALIGGDK 153

Query: 161 SSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRI 220
           S+FY  GF G+QDTLWD  GRHYFHRCTIQGAVDFIFGT QSIY+ C I V+G  L P +
Sbjct: 154 SAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCVIQVLGGQLEPGL 213

Query: 221 TGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWD 280
            G+ITAQGRT+P D NGF+F +C V G+G  FLGRPWRGYSRVIFYNSN ++++ P GWD
Sbjct: 214 AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYNSNLTDVVVPEGWD 273

Query: 281 PWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            W F G E QL +AE+GC+G G+++  RVKW KKLS   I+ L  +SF++  GW+++ P
Sbjct: 274 AWNFVGHENQLVFAEHGCFGSGANIGRRVKWVKKLSESAIQNLADLSFINRGGWVEDLP 332

BLAST of MC07g0521 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 405.6 bits (1041), Expect = 3.9e-113
Identity = 186/299 (62.21%), Postives = 227/299 (75.92%), Query Frame = 0

Query: 41  VVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRR 100
           V+VDQSGHGNF+TIQ AID VP NN  W  I VKAG+YREK+TIP  KP+I++ G GKR 
Sbjct: 29  VIVDQSGHGNFTTIQKAIDSVPINNTHWFFINVKAGLYREKITIPQKKPFIVIVGAGKRS 88

Query: 101 TRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWN---NGNPRVPAVAAMVTGDK 160
           TRV WDDH ++AQSPTF + ADN VVK I+F NSYN P N   N NPRVPAVAA + GDK
Sbjct: 89  TRVEWDDHASLAQSPTFATLADNTVVKKITFANSYNFPSNGKINKNPRVPAVAAFIGGDK 148

Query: 161 SSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRI 220
           S+FY  GF G+QDTLWD+ GRHYFHRCTIQGAVDFI G+ QSIY+ C I V+G  L P +
Sbjct: 149 SAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGAVDFILGSGQSIYQSCVIQVLGGQLGPGV 208

Query: 221 TGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWD 280
           TG+ITAQGRT+ ND NGFVF +C V G G  +LGR WR YSRVIFYNSN +++++P GW 
Sbjct: 209 TGYITAQGRTNANDANGFVFINCLVHGFGKAYLGRAWRPYSRVIFYNSNLTDVVDPLGWW 268

Query: 281 PWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            W + G E QLTYAE+GC+G GS+ S R KW KKLS+  ++ L  +SF++  GW+++ P
Sbjct: 269 EWNYQGYEKQLTYAEHGCFGSGSNTSRRAKWVKKLSASAVQHLADLSFINRGGWVEDLP 327

BLAST of MC07g0521 vs. TAIR 10
Match: AT2G19150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 1.2e-85
Identity = 148/299 (49.50%), Postives = 196/299 (65.55%), Query Frame = 0

Query: 39  RTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGK 98
           +T++V+ +    F T+Q+AID +P  N+ W+ IL+  G+Y EKVTIP  K YI ++G G 
Sbjct: 39  KTIIVNPNDARYFKTVQSAIDSIPLQNQDWIRILISNGIYSEKVTIPRGKGYIYMQGGGI 98

Query: 99  RRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKS 158
            +T + + DH     S TFTS   NI++  I+F+N YN   ++ +P  PAVAAM+ GDK 
Sbjct: 99  EKTIIAYGDHQLTNTSATFTSYPSNIIITGITFKNKYNIA-SSSSPTKPAVAAMMLGDKY 158

Query: 159 SFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISV-VGEALPPRI 218
           +     F G QDTL+D  GRHY+ RC I G +DFIFG AQSI+EGCT+ + VG   P  +
Sbjct: 159 AIIDSSFDGFQDTLYDDYGRHYYKRCVISGGIDFIFGGAQSIFEGCTLKLRVGIYPPNEV 218

Query: 219 TGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWD 278
            G ITAQGR  P D  GFVFKDC V+GSG   LGR W+ YSRVIFY S FS+ I P GWD
Sbjct: 219 YGTITAQGRDSPTDKGGFVFKDCTVMGSGKALLGRAWKSYSRVIFYRSMFSDNILPIGWD 278

Query: 279 PWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            W+  GQEG +T+ E+GC G G+D S RV W  K S +++ Q T+++F+D EGWL   P
Sbjct: 279 AWKAKGQEGHITFVEFGCTGVGADTSKRVPWLTKASEKDVLQFTNLTFIDEEGWLSRLP 336

BLAST of MC07g0521 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 9.7e-80
Identity = 143/308 (46.43%), Postives = 194/308 (62.99%), Query Frame = 0

Query: 30  SVGEKKVAWRTVVVDQSGHGNFSTIQAAIDDVPSNNRFWVSILVKAGVYREKVTIPYDKP 89
           S   +++A+ T+ VD +G GNF+T+Q+AID +   N  W+ +  + G+YREKVTIP +K 
Sbjct: 25  SAAAEQIAY-TITVDLNGGGNFTTVQSAIDSISPPNHNWIRVFTQNGIYREKVTIPKEKG 84

Query: 90  YIILKGQGKRRTRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGNPRVPAV 149
           +I L+G+G  +T + +DDH     S TFT+ AD+IV+  I+F+N+YN   NN    VPAV
Sbjct: 85  FIYLQGKGIEQTVIEYDDHQATDISATFTAFADDIVISGITFKNTYNIVPNNKREIVPAV 144

Query: 150 AAMVTGDKSSFYKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISV- 209
           AA + GD+       F GLQDTL+D  GRHY+ RC I G +DFIFG  QS+++ CT+++ 
Sbjct: 145 AARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGIDFIFGYGQSLFKECTLNMT 204

Query: 210 VGEALPPRITGFITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFS 269
           +G   P    G ITA  R  P+D  GFVF DC V G G T LGR W   +RVIF  S  S
Sbjct: 205 LGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTLLGRAWGSNARVIFDRSRLS 264

Query: 270 NIINPNGWDPWRFAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDT 329
           +++ P GWD WR  G E  LT+ E GC G G+D S RV W KKLS  E+    S+SF+D 
Sbjct: 265 DVVLPIGWDAWRAKGNERDLTFVEAGCTGAGADTSQRVPWLKKLSLSEVDGFASVSFIDQ 324

Query: 330 EGWLQNQP 337
           +GW+   P
Sbjct: 325 DGWISRFP 331

BLAST of MC07g0521 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 262.7 bits (670), Expect = 4.1e-70
Identity = 135/296 (45.61%), Postives = 173/296 (58.45%), Query Frame = 0

Query: 43  VDQSGHGNFSTIQAAIDDVPS--NNRFWVSILVKAGVYREKVTIPYDKPYIILKGQGKRR 102
           VDQSG G+FS IQ AI+ +P   NN     I VK G+YREKV IP +KPYI L G     
Sbjct: 53  VDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQASN 112

Query: 103 TRVVWDDHLNIAQSPTFTSSADNIVVKCISFENSYNDPWNNGNPRVPAVAAMVTGDKSSF 162
           T ++W D  +I +SPT T  A + V + ++ +N +            AVA  V  DK++F
Sbjct: 113 TFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGR-------AVALRVAADKAAF 172

Query: 163 YKCGFYGLQDTLWDALGRHYFHRCTIQGAVDFIFGTAQSIYEGCTISVVGEALPPRITGF 222
           Y C     QDTL D  G HYF  C I+GA DFI G+A S+YE C +     +L P   G 
Sbjct: 173 YGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHL----HSLSPN-NGS 232

Query: 223 ITAQGRTDPNDGNGFVFKDCKVVGSGSTFLGRPWRGYSRVIFYNSNFSNIINPNGWDPWR 282
           ITAQ RT   + +GF F  CK+ GSGSTFLGRPW  YSRV+F  S FSN++ P GW+ W 
Sbjct: 233 ITAQMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWG 292

Query: 283 FAGQEGQLTYAEYGCYGPGSDMSHRVKWEKKLSSEEIRQLTSMSFVDTEGWLQNQP 337
            + +E  + Y EY CYGPG+D   RV+W K+LS EE     S  F+  + WL+  P
Sbjct: 293 DSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAP 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4PSN06.2e-11663.21Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1[more]
Q3E9D35.5e-11262.21Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1[more]
O644791.7e-8449.50Putative pectinesterase 10 OS=Arabidopsis thaliana OX=3702 GN=PME10 PE=2 SV=1[more]
Q4PSQ51.4e-7846.43Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2[more]
Q9SIJ95.7e-6945.61Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022138633.11.60e-259100.00probable pectinesterase 29 [Momordica charantia][more]
XP_038875009.14.83e-20479.53probable pectinesterase 29 [Benincasa hispida][more]
XP_023005997.14.87e-19979.38probable pectinesterase 29 [Cucurbita maxima][more]
XP_022958865.11.62e-19778.15probable pectinesterase 29 [Cucurbita moschata][more]
XP_008457276.11.65e-19776.26PREDICTED: probable pectinesterase 29 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1CDJ57.77e-260100.00Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111009749 PE=4 SV=1[more]
A0A6J1KUQ12.36e-19979.38Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111498876 PE=4 SV=1[more]
A0A6J1H4P17.86e-19878.15Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111460019 PE=4 SV=1[more]
A0A1S3C5447.99e-19876.26Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497005 PE=4 SV=1[more]
W9SM623.91e-16970.29Pectinesterase OS=Morus notabilis OX=981085 GN=L484_021593 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G24130.14.4e-11763.21Pectin lyase-like superfamily protein [more]
AT5G18990.13.9e-11362.21Pectin lyase-like superfamily protein [more]
AT2G19150.11.2e-8549.50Pectin lyase-like superfamily protein [more]
AT2G47280.19.7e-8046.43Pectin lyase-like superfamily protein [more]
AT2G21610.14.1e-7045.61pectinesterase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 36..336
e-value: 6.0E-105
score: 353.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 41..328
e-value: 1.4E-68
score: 230.8
NoneNo IPR availablePANTHERPTHR31321:SF76PECTINESTERASE 10-RELATEDcoord: 25..336
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 25..336
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 186..195
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 38..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0521.1MC07g0521.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity