MC07g0394 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0394
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionFlap endonuclease GEN-like 1
LocationMC07: 11833964 .. 11837423 (-)
RNA-Seq ExpressionMC07g0394
SyntenyMC07g0394
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAGTCGGCGGCCACTTCTGGGATTTGCTGAAGCCTTACGCCCGAACTGAGGGCTTCGATTTCCTCAGGAACAAGCGGGTCGCCGTCGACCTCTCCTTCTGGATCGTCCAGCACGAGACCGCCATTAAAACCAACGCTCGAAGCCCCCATTTGAGGCTCTCCTTCTTCCGCACCGTCACTCTCTTTGCCAAGGTCTTGTTGCTCGTGTTTTTGTTTCTTTGCCTGCAAGAATTCGGCATGGAAATTCCTTGCCGGCAAAAATTTTAGAGAATTTTCGGGGTTTGTGATGTGCTTTTGCGATTCTGTTTGTTGCCTAGTGAGTAACATGAATGAATTTGAGAAGAAATTGGGAATCGCTATTTTCTCGTTTTCTTTTATTTTTGTTTTCTTTGTTGATGAGTATCCTATCAGTGAGTTATTGATTTATGTTAGGCTTTGATTCACAAACTGATGGTGATGCTGTTGTGTATTTTACAGTTTGGTGCGTTTCCAGTGTTCGTATTAGATGGAACTCCTTCGCCCTTAAAATCTAAGGCAAGGATTATGAGATTCTTTCGGTTATCTGGTATGGATGCTTCTGATCTCCCAAAGGTTGAAGATCGGATTTCAGTTGACAGAAACCGCAAATTTGCCAAATGTGTTAAAGAATGTGTGGTAGGATGTGCTTCACTCACTAATGACTATTTTTATCACCTATGCTCACCAATTTAGACTACATTTGATTTAATTTGTTTTCGATCGAGAATAATATGTTTTGTGCGTCAACTACGAAGGTTTTGGGATGTTTGGAAATTCCCTCCAACGTCCCGATTCAAGCCGCTAAGTGAAAAGCTAAAAAAATTTAATAGTCTTGTTTTCTCTCGGAGTTGACTTTGGGTCGGGTGAAGTCGTCTCCAGAGGTAGTGAGGTGGATCATGAGCATAATGCAAGGTGTGGAGTCACCTGATACAGTGGTTATAGAAAGGAAATGCTTTTGTGCATCTTGGGGTTTTGTTTTGTCTAGTGTCTACAATTTTATTCTAGATATTGAATTCCAAAGTCCTTGCAATGTTGATGAGCTTTGTCTCTGAATGTGCACCAACTTTTGGGTTAATTTGTGCAATCCTTTTCCATTTGTGTTACTCACTGCTGCATCTTTGGTGCTTTTGTTTGACTTCCAATGCTCATCATGTGTCTTACTAATAGTTGCTTCATAGGAACTGCTTGAGCTATTTGGGATGCCTGTTTTAGAAGCAAAAGGAGAAGCCGAAGCACTATGCGCCGAGTTGAATAGTAAAGGCTTTGTGGATGCCTGCATCACAGCTGACAGTGATGCTTTTCTGTTTGGGGCCAAATGTGTAATTAAATCCTTCCATCCCAATTCCAAAGCAAGTAATCCTATCTATACCAGTCTTCTTTATTTGAGACTTCTTTCTTTGTCTTTCCTATCGAGTATAATATACTAAGTTGTTGAGTATAAGAACATCTTCACATGCAGGAACCATTGGAATGCTACTTCATGTCAGATATTGAAGCTGCACTTGGGTTGAATAGGAAGCATTTGATAGCAATCTCTCTCCTGGTTGGAAATGACCATGACTTAAGTGGGGTGCAAGGAGTTGGGCTTGATACCGCTGTTCGTTTTGTCCAAGACTATACAGATGATGAGATTTTGAATAAGTAATAATTAGCTTACATGTTCTTTTTAAATACCAATAAGTAGATTTTCCGATTATTATTCGGCATTTTAATTGAGTTTGAACTGCTGAGTGTTTTGCAGATTGCATGAAATAGGAAACAAGGACAACAGGGTGCTTCAGGGTGGTCTTCAATTGGCAAACGACTGTGGACAATCTTCGGACGTGAACTTGAGAAAGATCAAGTGTTCTCACTGTTCATACTGTGGTCATCCAGGCAGCAAAAGAGCTCACGTTAAGTCTCCGTGCGAATACTGTGATGCAAGTCAAGGTGATGGTTGCGTAAAGAAGCCAGAAGGATTTAAATGCAATTGTTCCTCATGTGATACGGTACCTCATGATCATGTCCCTCTGATTTTTCTCTGCAATTTGGTCTGCTTAGATCACAATTTTTACTCATTGTGCTACCAGAATCCATTATTTCGTAAAATTTTCCGCTTTATTTGCTTCTTTTACATAAGCAACATTTGTTTTGGATTTTCCCAATATTGTTTAATAAGCTATTTTTCGATTCTTGATTGGCAGGATCGAGAAGAAAAGGAGAAAAAGAAGCAAGAAAATTGGCGATTAAGAGTTTGCAACAAGATTGCTGTGGAGCCAAAATTTCCCAGTAGCGAACTTATTAAAATGTATCTGTGTGACAATCATAGTCATTTCAGTGGTATGAGTGGTTACGTTTGCATTTTAAGTGATAAATATTGTTAATGGTGTCTTGATTTGATACATGTTGATGACCGAGGAATTAAAATCGTTTCGCTCTGGCAGCAGACGGCTGTTCGAAAACACCTCCATCATCTCCCTCTTTAAAAAAAGGAAAAAAAAAAAACTCTGAGAGCTGTTATCAGTGTCTATGATCAGATGTTTTAAACTTTTATCTTATTTGATCTCTTTCTACTGCAGATGACGACCTTATCTTATCCTGGGGGAGCCCGAGAACCGAAATGCTGGTTGATTTTTTAGTTTTTCATCTACGGTGGGAGCCATCTTACATTCGTCAGAGGATGCTTCCCATGCTATCCACCATTTTTCTCAGAGAAATGGCGAATAATCCAATTGGAACTTTGTTATACGGGCAGTATGAGTTCGATTCCATACTTCGAGAGAAAATAAGATATGGACATCAGTTTTATGTCGTCAAATGGAAAAAGGCTGTCCCTGCACTCAGCAATGTTATATACGAAGGCTCTTCTGAGGGATCCGGTACTGGGCCAGAAGATGGCATTGATGTCGACGAACCCGTGGATTTGACAGATGAATCTGACTCCCCGAAGATTCATATTCAAGACGGTTGCTCCTTTCTACTGACAGATGAAAATATGGACCTCGTTCGTGCTGCTTATCCCGAGCAAGCTGCCAAATTTTTGCATGAAAAGGTATCATCATTTCTTCCATCTTGATCTTTGTGGTAATGTTTTTTAGCTTCCCTTGGAGGGCTGTTTGAACTTTCAGTTGATGGCTTGTATTGTGTGTTTGTGATTTTATCTGATGGATTTCTAAAGCTGCAATGGATTATTCTCATATTTGAAACTTTATTGGAACTAAAGGAATTGAAAAAGCAGAAGAAATCAACCTCAACGCCTTGTGGGACGAGCGAAAAATCAGATTCATCGAAAGTGAAAGGTGTCCAGTTGAGTATTACCGAGTTCTACCGTTCAACCAAAGCACAACAAGCAGCAGAGAGAGTAGATTCGACAAATGAGGGTGAGGAGACGAGCACGGGAAAGAGGAAGGAATCAAGTACCACTCTTTCAAAGTCGGTAAGGCGTCGCCTTTTGTTTGAC

mRNA sequence

ATGGGAGTCGGCGGCCACTTCTGGGATTTGCTGAAGCCTTACGCCCGAACTGAGGGCTTCGATTTCCTCAGGAACAAGCGGGTCGCCGTCGACCTCTCCTTCTGGATCGTCCAGCACGAGACCGCCATTAAAACCAACGCTCGAAGCCCCCATTTGAGGCTCTCCTTCTTCCGCACCGTCACTCTCTTTGCCAAGTTTGGTGCGTTTCCAGTGTTCGTATTAGATGGAACTCCTTCGCCCTTAAAATCTAAGGCAAGGATTATGAGATTCTTTCGGTTATCTGGTATGGATGCTTCTGATCTCCCAAAGGTTGAAGATCGGATTTCAGTTGACAGAAACCGCAAATTTGCCAAATGTGTTAAAGAATGTGTGGAACTGCTTGAGCTATTTGGGATGCCTGTTTTAGAAGCAAAAGGAGAAGCCGAAGCACTATGCGCCGAGTTGAATAGTAAAGGCTTTGTGGATGCCTGCATCACAGCTGACAGTGATGCTTTTCTGTTTGGGGCCAAATGTGTAATTAAATCCTTCCATCCCAATTCCAAAGCAAAACATCTTCACATGCAGGAACCATTGGAATGCTACTTCATGTCAGATATTGAAGCTGCACTTGGGTTGAATAGGAAGCATTTGATAGCAATCTCTCTCCTGGTTGGAAATGACCATGACTTAAGTGGGGTGCAAGGAGTTGGGCTTGATACCGCTGTTCGTTTTGTCCAAGACTATACAGATGATGAGATTTTGAATAAATTGCATGAAATAGGAAACAAGGACAACAGGGTGCTTCAGGGTGGTCTTCAATTGGCAAACGACTGTGGACAATCTTCGGACGTGAACTTGAGAAAGATCAAGTGTTCTCACTGTTCATACTGTGGTCATCCAGGCAGCAAAAGAGCTCACGTTAAGTCTCCGTGCGAATACTGTGATGCAAGTCAAGGTGATGGTTGCGTAAAGAAGCCAGAAGGATTTAAATGCAATTGTTCCTCATGTGATACGGATCGAGAAGAAAAGGAGAAAAAGAAGCAAGAAAATTGGCGATTAAGAGTTTGCAACAAGATTGCTGTGGAGCCAAAATTTCCCAGTAGCGAACTTATTAAAATGTATCTGTGTGACAATCATAGTCATTTCAGTGATGACGACCTTATCTTATCCTGGGGGAGCCCGAGAACCGAAATGCTGGTTGATTTTTTAGTTTTTCATCTACGGTGGGAGCCATCTTACATTCGTCAGAGGATGCTTCCCATGCTATCCACCATTTTTCTCAGAGAAATGGCGAATAATCCAATTGGAACTTTGTTATACGGGCAGTATGAGTTCGATTCCATACTTCGAGAGAAAATAAGATATGGACATCAGTTTTATGTCGTCAAATGGAAAAAGGCTGTCCCTGCACTCAGCAATGTTATATACGAAGGCTCTTCTGAGGGATCCGGTACTGGGCCAGAAGATGGCATTGATGTCGACGAACCCGTGGATTTGACAGATGAATCTGACTCCCCGAAGATTCATATTCAAGACGGTTGCTCCTTTCTACTGACAGATGAAAATATGGACCTCGTTCGTGCTGCTTATCCCGAGCAAGCTGCCAAATTTTTGCATGAAAAGGAATTGAAAAAGCAGAAGAAATCAACCTCAACGCCTTGTGGGACGAGCGAAAAATCAGATTCATCGAAAGTGAAAGGTGTCCAGTTGAGTATTACCGAGTTCTACCGTTCAACCAAAGCACAACAAGCAGCAGAGAGAGTAGATTCGACAAATGAGGGTGAGGAGACGAGCACGGGAAAGAGGAAGGAATCAAGTACCACTCTTTCAAAGTCGGTAAGGCGTCGCCTTTTGTTTGAC

Coding sequence (CDS)

ATGGGAGTCGGCGGCCACTTCTGGGATTTGCTGAAGCCTTACGCCCGAACTGAGGGCTTCGATTTCCTCAGGAACAAGCGGGTCGCCGTCGACCTCTCCTTCTGGATCGTCCAGCACGAGACCGCCATTAAAACCAACGCTCGAAGCCCCCATTTGAGGCTCTCCTTCTTCCGCACCGTCACTCTCTTTGCCAAGTTTGGTGCGTTTCCAGTGTTCGTATTAGATGGAACTCCTTCGCCCTTAAAATCTAAGGCAAGGATTATGAGATTCTTTCGGTTATCTGGTATGGATGCTTCTGATCTCCCAAAGGTTGAAGATCGGATTTCAGTTGACAGAAACCGCAAATTTGCCAAATGTGTTAAAGAATGTGTGGAACTGCTTGAGCTATTTGGGATGCCTGTTTTAGAAGCAAAAGGAGAAGCCGAAGCACTATGCGCCGAGTTGAATAGTAAAGGCTTTGTGGATGCCTGCATCACAGCTGACAGTGATGCTTTTCTGTTTGGGGCCAAATGTGTAATTAAATCCTTCCATCCCAATTCCAAAGCAAAACATCTTCACATGCAGGAACCATTGGAATGCTACTTCATGTCAGATATTGAAGCTGCACTTGGGTTGAATAGGAAGCATTTGATAGCAATCTCTCTCCTGGTTGGAAATGACCATGACTTAAGTGGGGTGCAAGGAGTTGGGCTTGATACCGCTGTTCGTTTTGTCCAAGACTATACAGATGATGAGATTTTGAATAAATTGCATGAAATAGGAAACAAGGACAACAGGGTGCTTCAGGGTGGTCTTCAATTGGCAAACGACTGTGGACAATCTTCGGACGTGAACTTGAGAAAGATCAAGTGTTCTCACTGTTCATACTGTGGTCATCCAGGCAGCAAAAGAGCTCACGTTAAGTCTCCGTGCGAATACTGTGATGCAAGTCAAGGTGATGGTTGCGTAAAGAAGCCAGAAGGATTTAAATGCAATTGTTCCTCATGTGATACGGATCGAGAAGAAAAGGAGAAAAAGAAGCAAGAAAATTGGCGATTAAGAGTTTGCAACAAGATTGCTGTGGAGCCAAAATTTCCCAGTAGCGAACTTATTAAAATGTATCTGTGTGACAATCATAGTCATTTCAGTGATGACGACCTTATCTTATCCTGGGGGAGCCCGAGAACCGAAATGCTGGTTGATTTTTTAGTTTTTCATCTACGGTGGGAGCCATCTTACATTCGTCAGAGGATGCTTCCCATGCTATCCACCATTTTTCTCAGAGAAATGGCGAATAATCCAATTGGAACTTTGTTATACGGGCAGTATGAGTTCGATTCCATACTTCGAGAGAAAATAAGATATGGACATCAGTTTTATGTCGTCAAATGGAAAAAGGCTGTCCCTGCACTCAGCAATGTTATATACGAAGGCTCTTCTGAGGGATCCGGTACTGGGCCAGAAGATGGCATTGATGTCGACGAACCCGTGGATTTGACAGATGAATCTGACTCCCCGAAGATTCATATTCAAGACGGTTGCTCCTTTCTACTGACAGATGAAAATATGGACCTCGTTCGTGCTGCTTATCCCGAGCAAGCTGCCAAATTTTTGCATGAAAAGGAATTGAAAAAGCAGAAGAAATCAACCTCAACGCCTTGTGGGACGAGCGAAAAATCAGATTCATCGAAAGTGAAAGGTGTCCAGTTGAGTATTACCGAGTTCTACCGTTCAACCAAAGCACAACAAGCAGCAGAGAGAGTAGATTCGACAAATGAGGGTGAGGAGACGAGCACGGGAAAGAGGAAGGAATCAAGTACCACTCTTTCAAAGTCGGTAAGGCGTCGCCTTTTGTTTGAC

Protein sequence

MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTVTLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCVKECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNSKAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFLREMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQKKSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESSTTLSKSVRRRLLFD
Homology
BLAST of MC07g0394 vs. ExPASy Swiss-Prot
Match: Q9LPD2 (Flap endonuclease GEN-like 1 OS=Arabidopsis thaliana OX=3702 GN=GEN1 PE=1 SV=3)

HSP 1 Score: 627.1 bits (1616), Expect = 2.1e-178
Identity = 333/626 (53.19%), Postives = 442/626 (70.61%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGG+FWDLL+PYA+ +GFDFLRNKRVAVDLSFWIVQHETA+K     PHLRL+FFRT+
Sbjct: 1   MGVGGNFWDLLRPYAQQQGFDFLRNKRVAVDLSFWIVQHETAVKGFVLKPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
            LF+KFGA+PVFV+DGTPSPLKS+ARI RFFR SG+D  +LP ++D +SV+RN+ F++ V
Sbjct: 61  NLFSKFGAYPVFVVDGTPSPLKSQARISRFFRSSGIDTCNLPVIKDGVSVERNKLFSEWV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           +ECVELLEL G+PVL+A GEAEALCA+LNS+GFVDACIT DSDAFLFGA CVIK   PNS
Sbjct: 121 RECVELLELLGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           +       EP ECY MS IE+ LGL RKHLIAISLLVGND+D  GV G+G+D A+R V++
Sbjct: 181 R-------EPFECYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVRE 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           +++D++L +L +IGN     + GG++  +D G+     ++K +  HCS CGH GSKR H 
Sbjct: 241 FSEDQVLERLQDIGNGLQPAVPGGIKSGDD-GEEFRSEMKK-RSPHCSRCGHLGSKRTHF 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           KS CE+C      GC+KKP GF+C CS C  DR+ +E+KK  +W ++VC+KIA+ P+FP+
Sbjct: 301 KSSCEHCGCD--SGCIKKPLGFRCECSFCSKDRDLREQKKTNDWWIKVCDKIALAPEFPN 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
            ++I++YL D     + D   +SWG+P T MLVD +VF L W+PSY+R+ +LPMLSTI+L
Sbjct: 361 RKIIELYLSD--GLMTGDGSSMSWGTPDTGMLVDLMVFKLHWDPSYVRKMLLPMLSTIYL 420

Query: 421 REMANNPIG-TLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTG 480
           RE A N  G  LL  QYEF SI   K RYGHQ +V++W+K  P  ++      SE     
Sbjct: 421 REKARNNTGYALLCDQYEFHSIKCIKTRYGHQSFVIRWRK--PKSTSGYSHSHSE----- 480

Query: 481 PEDGIDV----DEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKE 540
           PE+ I V    +E VD  D  + P++   +G  FLLTDE + LV++A+P++   FLHEK+
Sbjct: 481 PEESIVVLEEEEESVDPLDGLNEPQVQNDNGDCFLLTDECIGLVQSAFPDETEHFLHEKK 540

Query: 541 LKKQKK------STSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEE 600
           L++ KK       T+TP  T+         GVQ SIT+FYRS K   A + +++    + 
Sbjct: 541 LRESKKKNVSEEETATPRATT--------MGVQRSITDFYRSAKKAAAGQSIETGGSSKA 598

Query: 601 TSTGKRKESSTT---LSKSVRRRLLF 613
           ++  KR+ +ST+   L+KSVRRRLLF
Sbjct: 601 SAEKKRQATSTSSSNLTKSVRRRLLF 598

BLAST of MC07g0394 vs. ExPASy Swiss-Prot
Match: Q64MA3 (Flap endonuclease GEN-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RAD PE=1 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 8.0e-162
Identity = 317/646 (49.07%), Postives = 408/646 (63.16%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKT---NARSPHLRLSFF 60
           MGVGG FWDLLKPYAR EG  +LR +RVAVDLSFW+V H  AI+    +AR PHLR  FF
Sbjct: 1   MGVGGSFWDLLKPYARHEGAGYLRGRRVAVDLSFWVVSHSAAIRARSPHARLPHLRTLFF 60

Query: 61  RTVTLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVD-----R 120
           RT++LF+K GAFPVFV+DG PSPLKS+ R  RFFR SGMD + LP  E   S D     R
Sbjct: 61  RTLSLFSKMGAFPVFVVDGQPSPLKSQVRAARFFRGSGMDLAALPSTEAEASADALVQPR 120

Query: 121 NRKFAKCVKECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCV 180
           N KF + V++CVELLE  GMPVL AKGE EALCA+LN++G VDACIT+DSDAFLFGAK V
Sbjct: 121 NAKFTRYVEDCVELLEYLGMPVLRAKGEGEALCAQLNNQGHVDACITSDSDAFLFGAKTV 180

Query: 181 IKSFHPNSKAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLD 240
           IK    N K       EP ECY M+DIE+ LGL RK ++A++LLVG+DHDL GV G G +
Sbjct: 181 IKVLRSNCK-------EPFECYNMADIESGLGLKRKQMVAMALLVGSDHDLHGVPGFGPE 240

Query: 241 TAVRFVQDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGH 300
           TA+RFVQ + +D +L KL+EIG      +       +D    S  +L + +  HCS+CGH
Sbjct: 241 TALRFVQLFDEDNVLAKLYEIGKGVYPFIGVSAPNIDDLPSPSTKSLPRARSPHCSHCGH 300

Query: 301 PGSKRAHVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKI 360
           PG+K+ H+K  C +C     + CV+KP GF C C SCD  R+ K +++ ENW+++VC +I
Sbjct: 301 PGNKKNHIKDGCNFCLVDSLENCVEKPAGFICECPSCDKARDLKVQRRNENWQIKVCKRI 360

Query: 361 AVEPKFPSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRML 420
           A E  FP+ E+I +YL D++    +   +L+W  P  E+LVDFL F   WEP+YIRQRML
Sbjct: 361 AAETNFPNEEIINLYLNDDNLDNENGVPLLTWNKPDMEILVDFLSFKQNWEPAYIRQRML 420

Query: 421 PMLSTIFLREMANNPIGT-LLYGQYEFDSILREKIRYGHQFYVVKWKKAVPA-LSN---- 480
           PMLSTI+LREMA++   + LLY QY+F SI R KIRYGH +Y+VKWK+   + +SN    
Sbjct: 421 PMLSTIYLREMASSQSKSFLLYDQYKFHSIQRIKIRYGHPYYLVKWKRVTRSMISNDPPS 480

Query: 481 ------------VIYEGSSEGSGTGPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDE 540
                        + +G  E      E+   + E  +L DE D P++   D   FLLTDE
Sbjct: 481 KQTELEGKNDKVEVLDGDDEVVDEEEEEPTMISETTELLDEPDVPQVLDDDKDCFLLTDE 540

Query: 541 NMDLVRAAYPEQAAKFLHEKELKKQK---KSTSTPCGTSEKSDSSKVKGVQLSITEFYRS 600
           +++LV AA+P++A +F  E+ LK+ K   + +       E     +  GVQLSI EFYRS
Sbjct: 541 DIELVNAAFPDEAQRFQEEQRLKEAKSIARKSKLNVAGFETPKGPRPSGVQLSIKEFYRS 600

Query: 601 TKAQQAAERVDSTNEGEETSTGKRKESSTTLS----KSVRRRLLFD 614
            K             G+    G RK S   LS    KSVRRRLLFD
Sbjct: 601 KKGL----------SGDSGKDGSRKSSDVDLSKNLPKSVRRRLLFD 629

BLAST of MC07g0394 vs. ExPASy Swiss-Prot
Match: Q8BMI4 (Flap endonuclease GEN homolog 1 OS=Mus musculus OX=10090 GN=Gen1 PE=1 SV=2)

HSP 1 Score: 194.5 bits (493), Expect = 3.5e-48
Identity = 157/565 (27.79%), Postives = 254/565 (44.96%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKT--NARSPHLRLSFFR 60
           MGV    W +L+P  +      L  K +AVDLS W+ + +T  K     + PHLR  FFR
Sbjct: 1   MGV-NDLWQILEPVKQHIHLQDLSGKTIAVDLSLWVCEAQTVKKMIGTVKKPHLRNLFFR 60

Query: 61  TVTLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAK 120
            ++   +     VFV++G P  LK+     R     G      P  + R        F  
Sbjct: 61  -ISYLTQMNVKLVFVMEGEPPMLKADVISKRTQTRYG------PSGKSRSQKTGRSHFKS 120

Query: 121 CVKECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHP 180
            ++EC+E+LE  GMP ++A GEAEA+CA LN+ G VD C+T D DAFL+GA+ V ++F  
Sbjct: 121 VLRECLEMLECLGMPWVQAAGEAEAMCAYLNASGHVDGCLTNDGDAFLYGAQTVYRNFTM 180

Query: 181 NSKAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFV 240
           N+K  H      ++CY +S I++ LGL+R  L+ +++L+G D+   GV GVG + A++ +
Sbjct: 181 NTKDPH------VDCYTISSIKSKLGLDRDALVGLAVLLGCDYLPKGVPGVGKEQALKLL 240

Query: 241 QDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRA 300
           Q +    +L + +              Q   D   S   +  K K  HCS C HPGS + 
Sbjct: 241 QIFKGQSLLQRFN--------------QWIEDPCYSVPQSAPK-KVVHCSVCSHPGSPKD 300

Query: 301 HVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKF 360
           H ++ C  C + +   C      + C C    TD      + + N + + C+       F
Sbjct: 301 HERNGCILCKSDK--YCEPHDYDYLCPCEWHQTDHNRHLSEIENNIKKKACSC----EGF 360

Query: 361 PSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTI 420
           P  E+I+ +L + +         +++  P   +   F V  + W   Y  +++L +L+  
Sbjct: 361 PFHEVIQEFLLNKNKMLKP----ITYQRPDLLLFQRFTVQKMEWPSHYACEKLLVLLTRY 420

Query: 421 FLREMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGT 480
              +M     G     Q +   I++ ++R G     ++W+K                   
Sbjct: 421 ---DMIERKHGRKTSNQLQPIRIVKPRVRNGVHCLEIEWEK------------------- 479

Query: 481 GPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKK 540
            PE  +         ++ D  K+        LLT E   L  AAYP+  A  +++K+L +
Sbjct: 481 -PEHYV--------VEDGDPGKLS-------LLTMEEASLFEAAYPDAVA--VYQKQLSE 479

Query: 541 QKKSTSTPCGTSEKSDSSKVKGVQL 564
            K       G  +KS  +K KG  L
Sbjct: 541 TK-------GRKQKSMKNKPKGSHL 479

BLAST of MC07g0394 vs. ExPASy Swiss-Prot
Match: Q17RS7 (Flap endonuclease GEN homolog 1 OS=Homo sapiens OX=9606 GN=GEN1 PE=1 SV=2)

HSP 1 Score: 188.0 bits (476), Expect = 3.3e-46
Identity = 151/549 (27.50%), Postives = 247/549 (44.99%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKT--NARSPHLRLSFFR 60
           MGV    W +L+P  +      L  K +AVDLS W+ + +T  K   +   PHLR  FFR
Sbjct: 1   MGV-NDLWQILEPVKQHIPLRNLGGKTIAVDLSLWVCEAQTVKKMMGSVMKPHLRNLFFR 60

Query: 61  TVTLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAK 120
            ++   +     VFV++G P  LK+     R     G       +   R        F  
Sbjct: 61  -ISYLTQMDVKLVFVMEGEPPKLKADVISKRNQSRYGSSGKSWSQKTGR------SHFKS 120

Query: 121 CVKECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHP 180
            ++EC+ +LE  G+P ++A GEAEA+CA LN+ G VD C+T D D FL+GA+ V ++F  
Sbjct: 121 VLRECLHMLECLGIPWVQAAGEAEAMCAYLNAGGHVDGCLTNDGDTFLYGAQTVYRNFTM 180

Query: 181 NSKAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFV 240
           N+K  H      ++CY MS I++ LGL+R  L+ +++L+G D+   GV GVG + A++ +
Sbjct: 181 NTKDPH------VDCYTMSSIKSKLGLDRDALVGLAILLGCDYLPKGVPGVGKEQALKLI 240

Query: 241 QDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRA 300
           Q      +L +     N+ N            C  S  + + K K +HCS C HPGS + 
Sbjct: 241 QILKGQSLLQRF----NRWNE---------TSCNSSPQLLVTK-KLAHCSVCSHPGSPKD 300

Query: 301 HVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKF 360
           H ++ C  C + +   C      + C C    T+ + +  + + N + + C        F
Sbjct: 301 HERNGCRLCKSDK--YCEPHDYEYCCPCEWHRTEHDRQLSEVENNIKKKAC----CCEGF 360

Query: 361 PSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTI 420
           P  E+I+ +L +          ++ +  P   +   F +  + W   Y  +++L +L+  
Sbjct: 361 PFHEVIQEFLLNKDKLVK----VIRYQRPDLLLFQRFTLEKMEWPNHYACEKLLVLLTHY 420

Query: 421 FLREMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGT 480
              +M    +G+    Q +   I++ +IR G   + ++W+K                   
Sbjct: 421 ---DMIERKLGSRNSNQLQPIRIVKTRIRNGVHCFEIEWEK------------------- 473

Query: 481 GPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEK-ELK 540
            PE     D+               Q G   LLT E   L  AAYPE  A +  +K E+K
Sbjct: 481 -PEHYAMEDK---------------QHGEFALLTIEEESLFEAAYPEIVAVYQKQKLEIK 473

Query: 541 KQKKSTSTP 547
            +K+    P
Sbjct: 541 GKKQKRIKP 473

BLAST of MC07g0394 vs. ExPASy Swiss-Prot
Match: Q9VRJ0 (Flap endonuclease GEN OS=Drosophila melanogaster OX=7227 GN=Gen PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.3e-38
Identity = 148/571 (25.92%), Postives = 250/571 (43.78%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNAR-SPHLRLSFFRT 60
           MGV    W +L P+   +  + LR K+VA+DL+ W+ +    +        HL+  FFRT
Sbjct: 1   MGV-KELWGVLTPHCERKPINELRGKKVAIDLAGWVCESLNVVDYFVHPRHHLKNLFFRT 60

Query: 61  VTLFAKFGAFPVFVLDGTPSPLKS----KARIMRFFRLSGMDASDLPKVEDRISVDRNRK 120
             L  +    PVFVL+G    LKS    K   ++F  +   ++ +  + +         +
Sbjct: 61  CYLIWE-QVTPVFVLEGVAPKLKSQVIAKRNELQFRGVKPKNSPECTQSQPSKGDKGRSR 120

Query: 121 FAKCVKECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKS 180
           F   +K+C  LL   G+  ++  GEAEA CA LN  G VD  I+ DSD F +GA  V ++
Sbjct: 121 FNHVLKQCETLLLSMGIQCVQGPGEAEAYCAFLNKHGLVDGVISQDSDCFAYGAVRVYRN 180

Query: 181 FHPNSKAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAV 240
           F  +++         ++ Y M +I + +   ++ +I ++LL G D+   G+ G+G D  +
Sbjct: 181 FSVSTQGAQAAAGGAVDIYDMREITSRMDFGQQKIIVMALLCGCDYCPDGIGGIGKDGVL 240

Query: 241 RFVQDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGS 300
           +    Y + EIL+++     + ++               + + +R    S CS CGH G 
Sbjct: 241 KLFNKYKETEILDRMRSWRGETDKY--------------NALEIRVDDKSICSNCGHIGK 300

Query: 301 KRAHVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVE 360
            ++H KS C  C   +G                CD +   KE++      L +  K  + 
Sbjct: 301 TQSHTKSGCSVCRTHKG----------------CD-ESLWKEQRLSIKSELTLRRKALLS 360

Query: 361 PKFPSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPML 420
           P FP+ E+I  +L +     +  +L L+W  P     +  +   L+W   Y  Q+  P+L
Sbjct: 361 PDFPNEEIIAEFLSEPD---TIPNLNLNWRQPNLVKFIKQIGHLLQWPEIYCFQKFFPIL 420

Query: 421 STIFLREMANNPIGTLLYGQYEFDSILREKIR-YGHQFYVVKWKKAVPALSNVIYEGSSE 480
           +                  +++     +EKI    H+    +  K VP+L    ++ S  
Sbjct: 421 T------------------RWQVQQSKQEKILIQPHEIIKKRTVKGVPSLELRWHDPSGI 480

Query: 481 GSGTGPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEK 540
             G  P+  I          E++ PK  I++      T E +D++  AYP+  A FL  K
Sbjct: 481 FKGLIPDKQI-------AEYEAEHPK-GIEE---LYYTIEPLDMLETAYPDLVAAFLKSK 506

Query: 541 E--LKK--QKKSTSTPCGTSEKSDSSKVKGV 562
           E   KK  +KK T++     E   +SK K V
Sbjct: 541 EKPAKKTTRKKKTASEEENKENEPNSKPKRV 506

BLAST of MC07g0394 vs. NCBI nr
Match: XP_022156153.1 (flap endonuclease GEN-like 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1231 bits (3184), Expect = 0.0
Identity = 613/613 (100.00%), Postives = 613/613 (100.00%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV
Sbjct: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS
Sbjct: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD
Sbjct: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV
Sbjct: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS
Sbjct: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420

Query: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480
           REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP
Sbjct: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480

Query: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540
           EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK
Sbjct: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540

Query: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600
           KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST
Sbjct: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600

Query: 601 TLSKSVRRRLLFD 613
           TLSKSVRRRLLFD
Sbjct: 601 TLSKSVRRRLLFD 613

BLAST of MC07g0394 vs. NCBI nr
Match: XP_022156154.1 (flap endonuclease GEN-like 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1209 bits (3127), Expect = 0.0
Identity = 606/613 (98.86%), Postives = 606/613 (98.86%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV
Sbjct: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS
Sbjct: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           K       EPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD
Sbjct: 181 K-------EPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV
Sbjct: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS
Sbjct: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420

Query: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480
           REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP
Sbjct: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480

Query: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540
           EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK
Sbjct: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540

Query: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600
           KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST
Sbjct: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600

Query: 601 TLSKSVRRRLLFD 613
           TLSKSVRRRLLFD
Sbjct: 601 TLSKSVRRRLLFD 606

BLAST of MC07g0394 vs. NCBI nr
Match: XP_038876411.1 (flap endonuclease GEN-like 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 537/635 (84.57%), Postives = 574/635 (90.39%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKRVAVDLSFWIVQHETAIK++ARSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRVAVDLSFWIVQHETAIKSSARSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPKVEDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PVLEAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KA--------------------KHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGND 240
           KA                    ++ HMQEPLECYF+SDIEAALGLNRKHLIAISLLVGND
Sbjct: 181 KASTPIYTSLLLYVTSFLPCSLEYHHMQEPLECYFISDIEAALGLNRKHLIAISLLVGND 240

Query: 241 HDLSGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLR 300
           H+L+GVQGVGLDTAVRFVQDYTDDEILNKL+EIGN+DN +LQGGL+L ND GQS  ++ R
Sbjct: 241 HNLNGVQGVGLDTAVRFVQDYTDDEILNKLYEIGNEDNSMLQGGLELVNDYGQSLKMSSR 300

Query: 301 KIKCSHCSYCGHPGSKRAHVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKK 360
           KIKCSHCS+CGHPGSKRAHVK PCEYCD S G+GC+KKP+GFKCNCSSCDTDREEKEKKK
Sbjct: 301 KIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKK 360

Query: 361 QENWRLRVCNKIAVEPKFPSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHL 420
           QENWRL+VC KIA+EP FPSSELI+MY CDNHS+FSDDDL+LSWGSPRTEMLVDFLVFHL
Sbjct: 361 QENWRLKVCKKIAMEPNFPSSELIQMYQCDNHSYFSDDDLVLSWGSPRTEMLVDFLVFHL 420

Query: 421 RWEPSYIRQRMLPMLSTIFLREMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKA 480
           RWEPSYIRQRMLPMLSTIFLREMANNPI TLLYGQYEFDSILREKIRYGH FYVVKWKKA
Sbjct: 421 RWEPSYIRQRMLPMLSTIFLREMANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKA 480

Query: 481 VPALSNVIYEGSSEGSGTGPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVR 540
           VP LSNVIYEGSSEG GTGP+D ID+DE VDLTDESDSPKIHIQDGCSFLLTDEN+DLV 
Sbjct: 481 VPTLSNVIYEGSSEGFGTGPDDAIDIDETVDLTDESDSPKIHIQDGCSFLLTDENIDLVG 540

Query: 541 AAYPEQAAKFLHEKELKKQKKSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQ-AAE 600
           AAYPEQAA FL EKE+K+QK  T+  CGTS KSDSSK KGVQLSITEFYR+TK QQ  +E
Sbjct: 541 AAYPEQAANFLQEKEMKRQKTPTTPACGTSGKSDSSKGKGVQLSITEFYRATKTQQQTSE 600

Query: 601 RVDSTNEGEETSTGKRKES-STTLSKSVRRRLLFD 613
             D TNE EETSTGKRK S S+TLSKSVRRRLLFD
Sbjct: 601 TTDLTNEDEETSTGKRKASTSSTLSKSVRRRLLFD 635

BLAST of MC07g0394 vs. NCBI nr
Match: XP_038876412.1 (flap endonuclease GEN-like 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1066 bits (2756), Expect = 0.0
Identity = 533/615 (86.67%), Postives = 568/615 (92.36%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKP+ARTEGFDFLRNKRVAVDLSFWIVQHETAIK++ARSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPHARTEGFDFLRNKRVAVDLSFWIVQHETAIKSSARSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPKVEDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PVLEAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNRKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           K       EPLECYF+SDIEAALGLNRKHLIAISLLVGNDH+L+GVQGVGLDTAVRFVQD
Sbjct: 181 K-------EPLECYFISDIEAALGLNRKHLIAISLLVGNDHNLNGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           YTDDEILNKL+EIGN+DN +LQGGL+L ND GQS  ++ RKIKCSHCS+CGHPGSKRAHV
Sbjct: 241 YTDDEILNKLYEIGNEDNSMLQGGLELVNDYGQSLKMSSRKIKCSHCSFCGHPGSKRAHV 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           K PCEYCD S G+GC+KKP+GFKCNCSSCDTDREEKEKKKQENWRL+VC KIA+EP FPS
Sbjct: 301 KFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLKVCKKIAMEPNFPS 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SELI+MY CDNHS+FSDDDL+LSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIQMYQCDNHSYFSDDDLVLSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420

Query: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480
           REMANNPI TLLYGQYEFDSILREKIRYGH FYVVKWKKAVP LSNVIYEGSSEG GTGP
Sbjct: 421 REMANNPIQTLLYGQYEFDSILREKIRYGHPFYVVKWKKAVPTLSNVIYEGSSEGFGTGP 480

Query: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540
           +D ID+DE VDLTDESDSPKIHIQDGCSFLLTDEN+DLV AAYPEQAA FL EKE+K+QK
Sbjct: 481 DDAIDIDETVDLTDESDSPKIHIQDGCSFLLTDENIDLVGAAYPEQAANFLQEKEMKRQK 540

Query: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQ-AAERVDSTNEGEETSTGKRKES- 600
             T+  CGTS KSDSSK KGVQLSITEFYR+TK QQ  +E  D TNE EETSTGKRK S 
Sbjct: 541 TPTTPACGTSGKSDSSKGKGVQLSITEFYRATKTQQQTSETTDLTNEDEETSTGKRKAST 600

Query: 601 STTLSKSVRRRLLFD 613
           S+TLSKSVRRRLLFD
Sbjct: 601 SSTLSKSVRRRLLFD 608

BLAST of MC07g0394 vs. NCBI nr
Match: KAA0040028.1 (flap endonuclease GEN-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 527/636 (82.86%), Postives = 566/636 (88.99%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKP ARTEGFDFLRNKRVAVDLSFWIVQHETAIK+ +RSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPKVEDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PVLEAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KA---------------------KHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGN 240
           KA                     ++ HMQEPLECYFMSDIEAALGLNR HLIAISLLVGN
Sbjct: 181 KACTPIYTSPLLQVTSLILPCSLEYHHMQEPLECYFMSDIEAALGLNRNHLIAISLLVGN 240

Query: 241 DHDLSGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNL 300
           DHDL+GVQGVGLDTAVRFVQDYTDD+ILNKL+EIGN+D   LQ G +L N+ GQSS+ + 
Sbjct: 241 DHDLNGVQGVGLDTAVRFVQDYTDDQILNKLYEIGNEDTTALQSGFELVNNYGQSSETDS 300

Query: 301 RKIKCSHCSYCGHPGSKRAHVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKK 360
           RKIKCSHCS+CGHPGSKRAHVK PCEYCD S G+GC+KKP+GFKCNCSSCDTDREEKEKK
Sbjct: 301 RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKK 360

Query: 361 KQENWRLRVCNKIAVEPKFPSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFH 420
           KQENWRLRVCNKIA+EP FPSSELI+MY CDNHS+FSDDDL+LSWGSPRTE+LVDFLVFH
Sbjct: 361 KQENWRLRVCNKIAMEPNFPSSELIQMYQCDNHSYFSDDDLVLSWGSPRTELLVDFLVFH 420

Query: 421 LRWEPSYIRQRMLPMLSTIFLREMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKK 480
           L+WEPSYIRQRMLPMLSTIFLREMANNPI TLL+GQYEFDSILREKIR+GH FYVVKWKK
Sbjct: 421 LQWEPSYIRQRMLPMLSTIFLREMANNPIQTLLFGQYEFDSILREKIRFGHSFYVVKWKK 480

Query: 481 AVPALSNVIYEGSSEGSGTGPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLV 540
           AVPA+SNV+YE S    GTGPED IDVDE VDLTDE DSPKIHIQDGCSFLLTDENM+LV
Sbjct: 481 AVPAISNVMYEDSLGEFGTGPEDAIDVDEAVDLTDEFDSPKIHIQDGCSFLLTDENMELV 540

Query: 541 RAAYPEQAAKFLHEKELKKQKKSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQ-AA 600
            AAYPEQAAKFL EKE+K+QK  ++  CGTS KSDSSK KGVQLSITEFYR+TK QQ  A
Sbjct: 541 GAAYPEQAAKFLQEKEMKRQKTPSTPACGTSGKSDSSKAKGVQLSITEFYRATKTQQQTA 600

Query: 601 ERVDSTNEGEETSTGKRK-ESSTTLSKSVRRRLLFD 613
           +  DSTNE EETSTGKRK  SS+TLSKSVRRRLLFD
Sbjct: 601 DTTDSTNEDEETSTGKRKASSSSTLSKSVRRRLLFD 636

BLAST of MC07g0394 vs. ExPASy TrEMBL
Match: A0A6J1DPU8 (flap endonuclease GEN-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023108 PE=4 SV=1)

HSP 1 Score: 1231 bits (3184), Expect = 0.0
Identity = 613/613 (100.00%), Postives = 613/613 (100.00%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV
Sbjct: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS
Sbjct: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD
Sbjct: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV
Sbjct: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS
Sbjct: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420

Query: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480
           REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP
Sbjct: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480

Query: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540
           EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK
Sbjct: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540

Query: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600
           KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST
Sbjct: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600

Query: 601 TLSKSVRRRLLFD 613
           TLSKSVRRRLLFD
Sbjct: 601 TLSKSVRRRLLFD 613

BLAST of MC07g0394 vs. ExPASy TrEMBL
Match: A0A6J1DSI1 (flap endonuclease GEN-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023108 PE=4 SV=1)

HSP 1 Score: 1209 bits (3127), Expect = 0.0
Identity = 606/613 (98.86%), Postives = 606/613 (98.86%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV
Sbjct: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS
Sbjct: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           K       EPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD
Sbjct: 181 K-------EPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV
Sbjct: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS
Sbjct: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420

Query: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480
           REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP
Sbjct: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480

Query: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540
           EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK
Sbjct: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540

Query: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600
           KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST
Sbjct: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEETSTGKRKESST 600

Query: 601 TLSKSVRRRLLFD 613
           TLSKSVRRRLLFD
Sbjct: 601 TLSKSVRRRLLFD 606

BLAST of MC07g0394 vs. ExPASy TrEMBL
Match: A0A5A7TAP1 (Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G003140 PE=4 SV=1)

HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 527/636 (82.86%), Postives = 566/636 (88.99%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKP ARTEGFDFLRNKRVAVDLSFWIVQHETAIK+ +RSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPKVEDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PVLEAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KA---------------------KHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGN 240
           KA                     ++ HMQEPLECYFMSDIEAALGLNR HLIAISLLVGN
Sbjct: 181 KACTPIYTSPLLQVTSLILPCSLEYHHMQEPLECYFMSDIEAALGLNRNHLIAISLLVGN 240

Query: 241 DHDLSGVQGVGLDTAVRFVQDYTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNL 300
           DHDL+GVQGVGLDTAVRFVQDYTDD+ILNKL+EIGN+D   LQ G +L N+ GQSS+ + 
Sbjct: 241 DHDLNGVQGVGLDTAVRFVQDYTDDQILNKLYEIGNEDTTALQSGFELVNNYGQSSETDS 300

Query: 301 RKIKCSHCSYCGHPGSKRAHVKSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKK 360
           RKIKCSHCS+CGHPGSKRAHVK PCEYCD S G+GC+KKP+GFKCNCSSCDTDREEKEKK
Sbjct: 301 RKIKCSHCSFCGHPGSKRAHVKFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKK 360

Query: 361 KQENWRLRVCNKIAVEPKFPSSELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFH 420
           KQENWRLRVCNKIA+EP FPSSELI+MY CDNHS+FSDDDL+LSWGSPRTE+LVDFLVFH
Sbjct: 361 KQENWRLRVCNKIAMEPNFPSSELIQMYQCDNHSYFSDDDLVLSWGSPRTELLVDFLVFH 420

Query: 421 LRWEPSYIRQRMLPMLSTIFLREMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKK 480
           L+WEPSYIRQRMLPMLSTIFLREMANNPI TLL+GQYEFDSILREKIR+GH FYVVKWKK
Sbjct: 421 LQWEPSYIRQRMLPMLSTIFLREMANNPIQTLLFGQYEFDSILREKIRFGHSFYVVKWKK 480

Query: 481 AVPALSNVIYEGSSEGSGTGPEDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLV 540
           AVPA+SNV+YE S    GTGPED IDVDE VDLTDE DSPKIHIQDGCSFLLTDENM+LV
Sbjct: 481 AVPAISNVMYEDSLGEFGTGPEDAIDVDEAVDLTDEFDSPKIHIQDGCSFLLTDENMELV 540

Query: 541 RAAYPEQAAKFLHEKELKKQKKSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQ-AA 600
            AAYPEQAAKFL EKE+K+QK  ++  CGTS KSDSSK KGVQLSITEFYR+TK QQ  A
Sbjct: 541 GAAYPEQAAKFLQEKEMKRQKTPSTPACGTSGKSDSSKAKGVQLSITEFYRATKTQQQTA 600

Query: 601 ERVDSTNEGEETSTGKRK-ESSTTLSKSVRRRLLFD 613
           +  DSTNE EETSTGKRK  SS+TLSKSVRRRLLFD
Sbjct: 601 DTTDSTNEDEETSTGKRKASSSSTLSKSVRRRLLFD 636

BLAST of MC07g0394 vs. ExPASy TrEMBL
Match: A0A5D3DLF9 (Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00140 PE=4 SV=1)

HSP 1 Score: 1046 bits (2704), Expect = 0.0
Identity = 523/615 (85.04%), Postives = 560/615 (91.06%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKP ARTEGFDFLRNKRVAVDLSFWIVQHETAIK+ +RSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPKVEDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PVLEAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           K       EPLECYFMSDIEAALGLNR HLIAISLLVGNDHDL+GVQGVGLDTAVRFVQD
Sbjct: 181 K-------EPLECYFMSDIEAALGLNRNHLIAISLLVGNDHDLNGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           YTDD+ILNKL+EIGN+D   LQ G +L N+ GQSS+ + RKIKCSHCS+CGHPGSKRAHV
Sbjct: 241 YTDDQILNKLYEIGNEDTTALQSGFELVNNYGQSSETDSRKIKCSHCSFCGHPGSKRAHV 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           K PCEYCD S G+GC+KKP+GFKCNCSSCDTDREEKEKKKQENWRLRVCNKIA+EP FPS
Sbjct: 301 KFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPS 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SELI+MY CDNHS+FSDDDL+LSWGSPRTE+LVDFLVFHL+WEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIQMYQCDNHSYFSDDDLVLSWGSPRTELLVDFLVFHLQWEPSYIRQRMLPMLSTIFL 420

Query: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480
           REMANNPI TLL+GQYEFDSILREKIR+GH FYVVKWKKAVPA+SNV+YE S    GTGP
Sbjct: 421 REMANNPIQTLLFGQYEFDSILREKIRFGHSFYVVKWKKAVPAISNVMYEDSLGEFGTGP 480

Query: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540
           ED IDVDE VDLTDE DSPKIHIQDGCSFLLTDENM+LV AAYPEQAAKFL EKE+K+QK
Sbjct: 481 EDAIDVDEAVDLTDEFDSPKIHIQDGCSFLLTDENMELVGAAYPEQAAKFLQEKEMKRQK 540

Query: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQ-AAERVDSTNEGEETSTGKRK-ES 600
             ++  CGTS KSDSSK KGVQLSITEFYR+TK QQ  A+  DSTNE EETSTGKRK  S
Sbjct: 541 TPSTPACGTSGKSDSSKAKGVQLSITEFYRATKTQQQTADTTDSTNEDEETSTGKRKASS 600

Query: 601 STTLSKSVRRRLLFD 613
           S+TLSKSVRRRLLFD
Sbjct: 601 SSTLSKSVRRRLLFD 608

BLAST of MC07g0394 vs. ExPASy TrEMBL
Match: A0A1S3CD60 (flap endonuclease GEN-like 1 OS=Cucumis melo OX=3656 GN=LOC103499055 PE=4 SV=1)

HSP 1 Score: 1045 bits (2701), Expect = 0.0
Identity = 522/615 (84.88%), Postives = 560/615 (91.06%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGGHFWDLLKP ARTEGFDFLRNKRVAVDLSFWIVQHETAIK+ +RSPHLRL+FFRT+
Sbjct: 1   MGVGGHFWDLLKPNARTEGFDFLRNKRVAVDLSFWIVQHETAIKSTSRSPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
           TLFAKFGAFPVFV+DGTPSPLKSKARI RFFRLSG+D SDLPKVEDRISV RNRKFAKCV
Sbjct: 61  TLFAKFGAFPVFVVDGTPSPLKSKARIARFFRLSGIDTSDLPKVEDRISVHRNRKFAKCV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           KECVELLELFG+PVLEAKGEAEALCAELN KGFVDACITADSDAFLFGAKCVIKS HPNS
Sbjct: 121 KECVELLELFGVPVLEAKGEAEALCAELNQKGFVDACITADSDAFLFGAKCVIKSIHPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           K       EPLECYFMSDIEAALGLNR HLIAISLLVGNDHDL+GVQGVGLDTAVRFVQD
Sbjct: 181 K-------EPLECYFMSDIEAALGLNRNHLIAISLLVGNDHDLNGVQGVGLDTAVRFVQD 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           YTDD+ILNKL+EIGN+D   LQ G +L N+ GQSS+ + RKIKCSHCS+CGHPGSKRAHV
Sbjct: 241 YTDDQILNKLYEIGNEDTTALQSGFELVNNYGQSSETDSRKIKCSHCSFCGHPGSKRAHV 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           K PCEYCD S G+GC+KKP+GFKCNCSSCDTDREEKEKKKQENWRLRVCNKIA+EP FPS
Sbjct: 301 KFPCEYCDVSHGEGCIKKPDGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAMEPNFPS 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
           SELI+MY CDNHS+FSDDDL+LSWGSPRTE+LVDFLVFHL+WEPSYIRQRMLPMLSTIFL
Sbjct: 361 SELIQMYQCDNHSYFSDDDLVLSWGSPRTELLVDFLVFHLQWEPSYIRQRMLPMLSTIFL 420

Query: 421 REMANNPIGTLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTGP 480
           REMANNPI TLL+GQYEFDSILREKIR+GH FYVVKWKKAVPA+SNV+YE S    GTGP
Sbjct: 421 REMANNPIQTLLFGQYEFDSILREKIRFGHSFYVVKWKKAVPAISNVMYEDSLGEFGTGP 480

Query: 481 EDGIDVDEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKELKKQK 540
           ED IDVDE VDLTDE DSPKIHIQDGCSFLLTDENM+LV AAYPEQAAKFL EKE+K+QK
Sbjct: 481 EDAIDVDEAVDLTDEFDSPKIHIQDGCSFLLTDENMELVGAAYPEQAAKFLQEKEMKRQK 540

Query: 541 KSTSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQ-AAERVDSTNEGEETSTGKRK-ES 600
             ++  CGTS KSDSSK KGVQLSITEFYR+TK QQ  A+  DSTNE EETSTGKR+  S
Sbjct: 541 TPSTPACGTSGKSDSSKAKGVQLSITEFYRATKTQQQTADTTDSTNEDEETSTGKRRASS 600

Query: 601 STTLSKSVRRRLLFD 613
           S+TLSKSVRRRLLFD
Sbjct: 601 SSTLSKSVRRRLLFD 608

BLAST of MC07g0394 vs. TAIR 10
Match: AT1G01880.1 (5'-3' exonuclease family protein )

HSP 1 Score: 627.1 bits (1616), Expect = 1.5e-179
Identity = 333/626 (53.19%), Postives = 442/626 (70.61%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGG+FWDLL+PYA+ +GFDFLRNKRVAVDLSFWIVQHETA+K     PHLRL+FFRT+
Sbjct: 1   MGVGGNFWDLLRPYAQQQGFDFLRNKRVAVDLSFWIVQHETAVKGFVLKPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
            LF+KFGA+PVFV+DGTPSPLKS+ARI RFFR SG+D  +LP ++D +SV+RN+ F++ V
Sbjct: 61  NLFSKFGAYPVFVVDGTPSPLKSQARISRFFRSSGIDTCNLPVIKDGVSVERNKLFSEWV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           +ECVELLEL G+PVL+A GEAEALCA+LNS+GFVDACIT DSDAFLFGA CVIK   PNS
Sbjct: 121 RECVELLELLGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           +       EP ECY MS IE+ LGL RKHLIAISLLVGND+D  GV G+G+D A+R V++
Sbjct: 181 R-------EPFECYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVRE 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           +++D++L +L +IGN     + GG++  +D G+     ++K +  HCS CGH GSKR H 
Sbjct: 241 FSEDQVLERLQDIGNGLQPAVPGGIKSGDD-GEEFRSEMKK-RSPHCSRCGHLGSKRTHF 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           KS CE+C      GC+KKP GF+C CS C  DR+ +E+KK  +W ++VC+KIA+ P+FP+
Sbjct: 301 KSSCEHCGCD--SGCIKKPLGFRCECSFCSKDRDLREQKKTNDWWIKVCDKIALAPEFPN 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
            ++I++YL D     + D   +SWG+P T MLVD +VF L W+PSY+R+ +LPMLSTI+L
Sbjct: 361 RKIIELYLSD--GLMTGDGSSMSWGTPDTGMLVDLMVFKLHWDPSYVRKMLLPMLSTIYL 420

Query: 421 REMANNPIG-TLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTG 480
           RE A N  G  LL  QYEF SI   K RYGHQ +V++W+K  P  ++      SE     
Sbjct: 421 REKARNNTGYALLCDQYEFHSIKCIKTRYGHQSFVIRWRK--PKSTSGYSHSHSE----- 480

Query: 481 PEDGIDV----DEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKE 540
           PE+ I V    +E VD  D  + P++   +G  FLLTDE + LV++A+P++   FLHEK+
Sbjct: 481 PEESIVVLEEEEESVDPLDGLNEPQVQNDNGDCFLLTDECIGLVQSAFPDETEHFLHEKK 540

Query: 541 LKKQKK------STSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEE 600
           L++ KK       T+TP  T+         GVQ SIT+FYRS K   A + +++    + 
Sbjct: 541 LRESKKKNVSEEETATPRATT--------MGVQRSITDFYRSAKKAAAGQSIETGGSSKA 598

Query: 601 TSTGKRKESSTT---LSKSVRRRLLF 613
           ++  KR+ +ST+   L+KSVRRRLLF
Sbjct: 601 SAEKKRQATSTSSSNLTKSVRRRLLF 598

BLAST of MC07g0394 vs. TAIR 10
Match: AT1G01880.2 (5'-3' exonuclease family protein )

HSP 1 Score: 620.9 bits (1600), Expect = 1.1e-177
Identity = 332/626 (53.04%), Postives = 441/626 (70.45%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKTNARSPHLRLSFFRTV 60
           MGVGG+FWDLL+PYA+ +GFDFLRNKRVAVDLSFWIVQHETA+K     PHLRL+FFRT+
Sbjct: 1   MGVGGNFWDLLRPYAQQQGFDFLRNKRVAVDLSFWIVQHETAVKGFVLKPHLRLTFFRTI 60

Query: 61  TLFAKFGAFPVFVLDGTPSPLKSKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCV 120
            LF+KFGA+PVFV+DGTPSPLKS+ARI RFFR SG+D  +LP ++D +SV+RN+ F++ V
Sbjct: 61  NLFSKFGAYPVFVVDGTPSPLKSQARISRFFRSSGIDTCNLPVIKDGVSVERNKLFSEWV 120

Query: 121 KECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNS 180
           +EC ELLEL G+PVL+A GEAEALCA+LNS+GFVDACIT DSDAFLFGA CVIK   PNS
Sbjct: 121 REC-ELLELLGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNS 180

Query: 181 KAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQD 240
           +       EP ECY MS IE+ LGL RKHLIAISLLVGND+D  GV G+G+D A+R V++
Sbjct: 181 R-------EPFECYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVRE 240

Query: 241 YTDDEILNKLHEIGNKDNRVLQGGLQLANDCGQSSDVNLRKIKCSHCSYCGHPGSKRAHV 300
           +++D++L +L +IGN     + GG++  +D G+     ++K +  HCS CGH GSKR H 
Sbjct: 241 FSEDQVLERLQDIGNGLQPAVPGGIKSGDD-GEEFRSEMKK-RSPHCSRCGHLGSKRTHF 300

Query: 301 KSPCEYCDASQGDGCVKKPEGFKCNCSSCDTDREEKEKKKQENWRLRVCNKIAVEPKFPS 360
           KS CE+C      GC+KKP GF+C CS C  DR+ +E+KK  +W ++VC+KIA+ P+FP+
Sbjct: 301 KSSCEHCGCD--SGCIKKPLGFRCECSFCSKDRDLREQKKTNDWWIKVCDKIALAPEFPN 360

Query: 361 SELIKMYLCDNHSHFSDDDLILSWGSPRTEMLVDFLVFHLRWEPSYIRQRMLPMLSTIFL 420
            ++I++YL D     + D   +SWG+P T MLVD +VF L W+PSY+R+ +LPMLSTI+L
Sbjct: 361 RKIIELYLSD--GLMTGDGSSMSWGTPDTGMLVDLMVFKLHWDPSYVRKMLLPMLSTIYL 420

Query: 421 REMANNPIG-TLLYGQYEFDSILREKIRYGHQFYVVKWKKAVPALSNVIYEGSSEGSGTG 480
           RE A N  G  LL  QYEF SI   K RYGHQ +V++W+K  P  ++      SE     
Sbjct: 421 REKARNNTGYALLCDQYEFHSIKCIKTRYGHQSFVIRWRK--PKSTSGYSHSHSE----- 480

Query: 481 PEDGIDV----DEPVDLTDESDSPKIHIQDGCSFLLTDENMDLVRAAYPEQAAKFLHEKE 540
           PE+ I V    +E VD  D  + P++   +G  FLLTDE + LV++A+P++   FLHEK+
Sbjct: 481 PEESIVVLEEEEESVDPLDGLNEPQVQNDNGDCFLLTDECIGLVQSAFPDETEHFLHEKK 540

Query: 541 LKKQKK------STSTPCGTSEKSDSSKVKGVQLSITEFYRSTKAQQAAERVDSTNEGEE 600
           L++ KK       T+TP  T+         GVQ SIT+FYRS K   A + +++    + 
Sbjct: 541 LRESKKKNVSEEETATPRATT--------MGVQRSITDFYRSAKKAAAGQSIETGGSSKA 597

Query: 601 TSTGKRKESSTT---LSKSVRRRLLF 613
           ++  KR+ +ST+   L+KSVRRRLLF
Sbjct: 601 SAEKKRQATSTSSSNLTKSVRRRLLF 597

BLAST of MC07g0394 vs. TAIR 10
Match: AT3G48900.2 (single-stranded DNA endonuclease family protein )

HSP 1 Score: 123.6 bits (309), Expect = 5.4e-28
Identity = 89/260 (34.23%), Postives = 128/260 (49.23%), Query Frame = 0

Query: 1   MGVGGHFWDLLKPYARTEGFDFLRNKRVAVDLSFWIVQHETAIKT---NARSPHLRLSFF 60
           MGV  + WD+L+P  +T   D L+NKRV VDLS W+V+     K+        +LR  FF
Sbjct: 1   MGV-KYLWDVLEPCKKTFPLDHLQNKRVCVDLSCWMVELHKVNKSYCATKEKVYLR-GFF 60

Query: 61  RTVTLFAKFGAFPVFVLDGTPSPLK---SKARIMRFFRLSGMDASDLPKVEDRISVDRNR 120
             +          + V DG    +K    K R+   F ++  D    P  E  +  +   
Sbjct: 61  HRLRALIALNCSIILVSDGAIPGIKVPTYKRRLKARFEIA--DDGVEPSKETSLKRNMGS 120

Query: 121 KFAKCVKECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITADSDAFLFGAKCVIK 180
           +F+  +KE   +    G+  L+   EAEA CA LNS+   DAC + DSD FLFGAK V +
Sbjct: 121 EFSCIIKEAKVIASTLGILCLDGIEEAEAQCALLNSESLCDACFSFDSDIFLFGAKTVYR 180

Query: 181 SFHPNSKAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDHDLSGVQGVGLDTA 240
                     L     + CY M DI+  LGL R  LIA++LL+G+D+   GV+G+  + A
Sbjct: 181 EI-------CLGEGGYVVCYEMDDIKKKLGLGRNSLIALALLLGSDYS-QGVRGLRQEKA 240

Query: 241 VRFVQDYTDDEILNKLHEIG 255
              V+   D+ IL K+   G
Sbjct: 241 CELVRSIGDNVILEKVASEG 248

BLAST of MC07g0394 vs. TAIR 10
Match: AT3G28030.1 (5'-3' exonuclease family protein )

HSP 1 Score: 100.9 bits (250), Expect = 3.7e-21
Identity = 57/170 (33.53%), Postives = 92/170 (54.12%), Query Frame = 0

Query: 83   SKARIMRFFRLSGMDASDLPKVEDRISVDRNRKFAKCVKECVELLELFGMPVLEAKGEAE 142
            S+A I    R+   +   L   + ++  +     ++   EC ELL++FG+P + A  EAE
Sbjct: 889  SEANIEEEIRVLDQEFVSLGDEQRKLERNAESVSSEMFAECQELLQIFGIPYIIAPMEAE 948

Query: 143  ALCAELNSKGFVDACITADSDAFLFGAKCVIKSFHPNSKAKHLHMQEPLECYFMSDIEAA 202
            A CA +     VD  +T DSD FLFGA+ V K+   + K         +E YFM DIE  
Sbjct: 949  AQCAFMEQSNLVDGIVTDDSDVFLFGARSVYKNIFDDRKY--------VETYFMKDIEKE 1008

Query: 203  LGLNRKHLIAISLLVGNDHDLSGVQGVGLDTAVRFVQDYTDDEILNKLHE 253
            LGL+R  +I +++L+G+D+   G+ G+G+  A+  V  + +++ L K  E
Sbjct: 1009 LGLSRDKIIRMAMLLGSDY-TEGISGIGIVNAIEVVTAFPEEDGLQKFRE 1049

BLAST of MC07g0394 vs. TAIR 10
Match: AT3G48900.1 (single-stranded DNA endonuclease family protein )

HSP 1 Score: 88.2 bits (217), Expect = 2.5e-17
Identity = 56/153 (36.60%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 102 PKVEDRISVDRNRKFAKCVKECVELLELFGMPVLEAKGEAEALCAELNSKGFVDACITAD 161
           P  E  +  +   +F+  +KE   +    G+  L+   EAEA CA LNS+   DAC + D
Sbjct: 40  PSKETSLKRNMGSEFSCIIKEAKVIASTLGILCLDGIEEAEAQCALLNSESLCDACFSFD 99

Query: 162 SDAFLFGAKCVIKSFHPNSKAKHLHMQEPLECYFMSDIEAALGLNRKHLIAISLLVGNDH 221
           SD FLFGAK V +          L     + CY M DI+  LGL R  LIA++LL+G+D+
Sbjct: 100 SDIFLFGAKTVYREI-------CLGEGGYVVCYEMDDIKKKLGLGRNSLIALALLLGSDY 159

Query: 222 DLSGVQGVGLDTAVRFVQDYTDDEILNKLHEIG 255
              GV+G+  + A   V+   D+ IL K+   G
Sbjct: 160 S-QGVRGLRQEKACELVRSIGDNVILEKVASEG 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LPD22.1e-17853.19Flap endonuclease GEN-like 1 OS=Arabidopsis thaliana OX=3702 GN=GEN1 PE=1 SV=3[more]
Q64MA38.0e-16249.07Flap endonuclease GEN-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=RAD PE=... [more]
Q8BMI43.5e-4827.79Flap endonuclease GEN homolog 1 OS=Mus musculus OX=10090 GN=Gen1 PE=1 SV=2[more]
Q17RS73.3e-4627.50Flap endonuclease GEN homolog 1 OS=Homo sapiens OX=9606 GN=GEN1 PE=1 SV=2[more]
Q9VRJ04.3e-3825.92Flap endonuclease GEN OS=Drosophila melanogaster OX=7227 GN=Gen PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022156153.10.0100.00flap endonuclease GEN-like 1 isoform X1 [Momordica charantia][more]
XP_022156154.10.098.86flap endonuclease GEN-like 1 isoform X2 [Momordica charantia][more]
XP_038876411.10.084.57flap endonuclease GEN-like 1 isoform X1 [Benincasa hispida][more]
XP_038876412.10.086.67flap endonuclease GEN-like 1 isoform X2 [Benincasa hispida][more]
KAA0040028.10.082.86flap endonuclease GEN-like 1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1DPU80.0100.00flap endonuclease GEN-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1DSI10.098.86flap endonuclease GEN-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A5A7TAP10.082.86Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A5D3DLF90.085.04Flap endonuclease GEN-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CD600.084.88flap endonuclease GEN-like 1 OS=Cucumis melo OX=3656 GN=LOC103499055 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G01880.11.5e-17953.195'-3' exonuclease family protein [more]
AT1G01880.21.1e-17753.045'-3' exonuclease family protein [more]
AT3G48900.25.4e-2834.23single-stranded DNA endonuclease family protein [more]
AT3G28030.13.7e-2133.535'-3' exonuclease family protein [more]
AT3G48900.12.5e-1736.60single-stranded DNA endonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006084XPG/Rad2 endonucleasePRINTSPR00853XPGRADSUPERcoord: 210..225
score: 27.93
coord: 25..39
score: 38.12
coord: 70..89
score: 39.38
coord: 127..144
score: 44.62
coord: 148..168
score: 44.79
IPR006084XPG/Rad2 endonucleasePANTHERPTHR11081FLAP ENDONUCLEASE FAMILY MEMBERcoord: 1..601
IPR006086XPG-I domainSMARTSM00484xpgineucoord: 128..205
e-value: 6.0E-14
score: 62.3
IPR006086XPG-I domainPFAMPF00867XPG_Icoord: 129..220
e-value: 5.5E-23
score: 81.2
IPR006085XPG, N-terminalSMARTSM00485xpgn3coord: 1..92
e-value: 3.4E-4
score: 23.8
IPR006085XPG, N-terminalPFAMPF00752XPG_Ncoord: 1..86
e-value: 1.4E-8
score: 35.1
NoneNo IPR availableGENE3D3.40.50.1010coord: 1..199
e-value: 3.6E-40
score: 139.7
NoneNo IPR availableGENE3D1.10.150.20coord: 205..282
e-value: 2.1E-7
score: 32.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 472..498
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 542..559
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 572..613
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 534..559
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 582..603
NoneNo IPR availablePANTHERPTHR11081:SF59FLAP ENDONUCLEASE GEN-LIKE 1coord: 1..601
NoneNo IPR availableCDDcd09869PIN_GEN1coord: 2..209
e-value: 3.59236E-81
score: 253.295
IPR0362795'-3' exonuclease, C-terminal domain superfamilySUPERFAMILY478075' to 3' exonuclease, C-terminal subdomaincoord: 205..422
IPR029060PIN-like domain superfamilySUPERFAMILY88723PIN domain-likecoord: 6..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0394.1MC07g0394.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0009555 pollen development
cellular_component GO:0005634 nucleus
molecular_function GO:0017108 5'-flap endonuclease activity
molecular_function GO:0003690 double-stranded DNA binding
molecular_function GO:0003697 single-stranded DNA binding
molecular_function GO:0004518 nuclease activity