MC07g0384 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0384
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmethionine adenosyltransferase 2 subunit beta
LocationMC07: 11696197 .. 11700087 (-)
RNA-Seq ExpressionMC07g0384
SyntenyMC07g0384
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCAGGAAGAAGGTGCTGGTAGTGGGCGGCACAGGTTACTTAGGTCAGCATCTTCTATCAGGATTTTCCGGGATCGAAGGAGCTCCATACGATATCGCATTCACTTACCACTCCTTTCCGCCGGAAGATTTGTTGCGAGCTCTTCCTAATCTGCTCGCATTTCACGTCGATTTAAAATCCGGTCAAGGATTCGATGCCATTGCTGAGAAATTTGGCCAGGTTTGCCTCTGCCCTTTTTGCCGCTTGAAGTTGAATAGTTGAATTTGTGATCTGCTGTTATAAATGTAGAATTTCTAGTGCTGAAATATGATTTCGACATACCAGCCCGATGTTGTTGTTAATTGTGCTGCTATCTCTGTACCTCGTGCCTGCGAAACAGACCCTGCTTCTGCATTGTCGATCAACGTGCCTACTGCCCTTGGAAATTGGATGTTGAGCTTTGAAGAGAGGAATACTCTTCTAGTTCATCTATCAACTGATCAAGGTAACTCCTCCCTTCTTTGTCATTGAAGTAACTCTTCACTATTTATTCTTTCAACTTAGTCTCTGTAGTTTAAAATTTGCAAAAGAAGAAATTATTGGTTGGTGGGATCCAAACGGACTGGATTTTAAACTTTTAAACTACGGGGACTAAATTCTTACATGTTAAAGGTTTAGAGACTAGATGGTTGTAAATTAAAAATCTATTAGAGACATCAGACCAGGTGATTTGGAGTTTAGGAGGTATTTGAGTGCTTTAATAGAGAAAGGGCTTGACATTTCACTGTTTGTTGTGGGGTTGTTACAGTTTGGCCGACCTTACAACTAGAAAAGGGGGCAAAATCACAAGTTATCTTTAGAAAGTTGAGATCTTCTTGACAATCCTCTTGTCCCTTGAAGAAGGGCTAGTTTGGGAACAAACCTAGAAAGTTATGTCTATAGATAAGAAGATGAATCTTGAGCTGGCCAAGTTTATTCTGACTAAGTAGTTACGTTATAAAGCTTAGAGACTAAGTAGTTACACACTATAAACTAAAGGGATCAGTTATATACTGAAGTTTAATAGAGAAAGGGCTTGACATTTCACTATTTGTTGTGGGGTTGTTATAGTTTGGCCGACCTTACAACTAGAAAAGGGGGCAAAATCACAAGTTATCTTTAGAAAATTGAGATCTTCTTGACGATCCTCTTGTCCCTTGAAGAAGGGCTAGTTTGGGAACAAACCTAGAAAGTTATGTCTATAGATAAGAAGATGAATCTTGAGCTGGCCAAGGCCAAGTTCATTCAGACTAAGTAGTTACGTCATAAAGCTTAGAGACTAAGTAGTTACACACTATCAACTAAAGGGATCAGTTATATACTGAAGTTTAGGGATGACATTGCTGTTTTGATGAACTTATTGGTATCAAATATGTTTCTCAAAGTTATTATTTTTCTATGTTTATGTTTTGTTTTATCTTTGTCTTTCGAGTCTACTGAACTTCTCCTTCATCAACGGTATCTTTATGCCTAAGATTGTTGGCAAAAGATTAATGAGCATAGCCTGGGATGGTGCTACTCCATTAAAAACTTTGATGCCCCAACTTTTTCGGCCAATAGAGTCAATGAATTAGATTGAATTAGGAATTATAATCCCAGTTCAGTTATCTAGATGTCCAAATATGAAGATTTCGGTCTGCTGACTATAAGTAACTGATAATCATCATTACTAATTGGCTCTCTGGAGTAAAGTTTCATTAAATATAAGATTTTATTTCTGTCTATTTGAGGGGACATCTTTTTCCTTACTTTATGATTGTTATAAATTATTGAATAGTGCATGATGTTATTTTCCCACCAGTTTATGAAGGCGTGAGATCATTCTACAAGGAAGAAGATGAAACCATTCCTGTGAATGTTTATGGGAAGTCAAAGCTAGCAGCTGAACAGTATGTTTCTGAGAAATTTCCTAACTTTGCAATCTTGAGATCTAGCATCATTTTTGGTCCCCAGACCATCTCACCTGTTCCTAAATCTCTTCCAATTCAGGTACATGGTCGCATGCTATATTGTACTTCTGAAATTTCCTGATATATTAATCCATATATAATCATTTTGAATTCTAAGCACTGATGACGAATGTCTGCAAGTTCAAGCATATCGATCAGGCCATTAAAAACAACTAGTGGACATTAGGAAGAGAATTACATCCATCATAATTTGTTACTAAACATAAACTTATATAAACCAACTAACTTGATTGATTCTTTTTTTATTCAGTACAAAATATTTTGATGGCCTTCATGACTTCTTTGTATATGACAATTAATTTGTATCATTGTATGCATTCAATACAGTGGATTGATGCTGTTTTAACCAAAGGAAGCGAAGTTGACTTCTTTCACGATGAGTTTCGATGTCCCGTGTATGTCAAGGACGTTGTAGCTGTCATTATAAAATTGATTAAGACATGGATATCAGGTATGGATTTTGTAGGGACGTGATGGATGATAATACAGATGGTGCTCCCTAAGTCACCTTCAGTTAGTTCCTTTCATGTTGAATAAATTATAGTTTAAAAAACGTGGAATAAAAAATGGATAGTTCCCAATTTTTGTCATTTAGTAACTATTTCTCCATTAGGGTGTTTTTATTACATCTTCCTGAATCTTTAGCTTTATTTGCAATTTTATTTGTACTTTACTCTTTCGAGAGTTGTATCTTTGAACAATTTTGTTCGTTTTCATTAATTTAATGAAGAGTTAGTATCTTGTTCGAACAAAAAAAAAAGAAAAAGTAACTATTTCTCCATTGGTATTGCTTTATTTTTTGTCAGTTCTGTTGGCATATACTGCATTGCTTGATTGATAGTTGTTGTTCAATCATTTTTCTGCTAAGTTTTGACATGTTTGTATTTCTTTTTCCTTTATTATATTAATTTAGTTTCTTACTTCTGCAATAATTGATTATCGAATTCCAGAGAGCAAGCAAATGCAGTTGCTGTTAAATGTTGGAGGACCGAATCGTGTATCACGTGTCGAAATGGCCGAGACTGTTGCAGAAATTAGAGGGCACAACAAATCATTGATAAAAAGAGTGTCTGCATCCTCGGTATGTTCCTCTTGATCTTCCTATAATATCTTTTTCAAATGGTGTTAAGCTGTATTCATTTTTAGAACCAAAGACGCAGTAGTATAACCTATAGAATAGATCTTGTTCAAGTAAGGTCATAAGTTGTATTCCTTTTATCATAGATTAAACCATTTGATATCGGGATGTTCACGAGTTGGGTTGGGTCGGATTAGAAAAGTTTTTTGGACCAACCCGAAAATTCGGGTTAGTGAGTTCGTCTACCCGATGAAACTGAAACTTATTGCCAACCCAACCGTGAGAGAACTACGCTGAGTTCTTCCCCATAAACTATTTCCTAAAAGATACCCCTTCCTCTCCCTAATTCTTCCATTTATAAGTTTTTAACCAACTCATACCTAATTAGTTACTATAACGCCCCTAACTCATATACTAATACTATTTTCAAGCTATCCCTACCAGGACTCTCACAGAAACCTCCCAAAGATTAGAGGGGTGGACCTTGAGCATGATGCAAGGAGTGGAGTCCTGATACCAAATTTTAGAAACTAGAAAAATACTGAATACTTGAAACCGCACCATTTTTAGGTAGCACTGTTCTTTTTCGGTAGGCTAAATATAGTGCTGGAAATTTTAGATATTTTGGCAGCCTTGTCTTCCAGAAGATTCGTCTATTATTTCTACTTTTGGTTTGAGTTTCCACTTGAATGGTTTCAGATTGATCGAGGGGTAAAATCCCCTGCTGACATTTCAATGAACATCAGTAAACTGGTTCAGAAACTTGCCATTTCTCCCGTTTCATTCACTCATGGTGTCAGATTGACACTTGCAGCCGAGACT

mRNA sequence

ATGAGCAGGAAGAAGGTGCTGGTAGTGGGCGGCACAGGTTACTTAGGTCAGCATCTTCTATCAGGATTTTCCGGGATCGAAGGAGCTCCATACGATATCGCATTCACTTACCACTCCTTTCCGCCGGAAGATTTGTTGCGAGCTCTTCCTAATCTGCTCGCATTTCACGTCGATTTAAAATCCGGTCAAGGATTCGATGCCATTGCTGAGAAATTTGGCCAGCCCGATGTTGTTGTTAATTGTGCTGCTATCTCTGTACCTCGTGCCTGCGAAACAGACCCTGCTTCTGCATTGTCGATCAACGTGCCTACTGCCCTTGGAAATTGGATGTTGAGCTTTGAAGAGAGGAATACTCTTCTAGTTCATCTATCAACTGATCAAGTTTATGAAGGCGTGAGATCATTCTACAAGGAAGAAGATGAAACCATTCCTGTGAATGTTTATGGGAAGTCAAAGCTAGCAGCTGAACAGTATGTTTCTGAGAAATTTCCTAACTTTGCAATCTTGAGATCTAGCATCATTTTTGGTCCCCAGACCATCTCACCTGTTCCTAAATCTCTTCCAATTCAGTGGATTGATGCTGTTTTAACCAAAGGAAGCGAAGTTGACTTCTTTCACGATGAGTTTCGATGTCCCGTGTATGTCAAGGACGTTGTAGCTGTCATTATAAAATTGATTAAGACATGGATATCAGAGAGCAAGCAAATGCAGTTGCTGTTAAATGTTGGAGGACCGAATCGTGTATCACGTGTCGAAATGGCCGAGACTGTTGCAGAAATTAGAGGGCACAACAAATCATTGATAAAAAGAGTGTCTGCATCCTCGGTATCCTTGTCTTCCAGAAGATTCGTCTATTATTTCTACTTTTGGTTTGAGTTTCCACTTGAATGGTTTCAGATTGATCGAGGGGTAAAATCCCCTGCTGACATTTCAATGAACATCAGTAAACTGGTTCAGAAACTTGCCATTTCTCCCGTTTCATTCACTCATGGTGTCAGATTGACACTTGCAGCCGAGACT

Coding sequence (CDS)

ATGAGCAGGAAGAAGGTGCTGGTAGTGGGCGGCACAGGTTACTTAGGTCAGCATCTTCTATCAGGATTTTCCGGGATCGAAGGAGCTCCATACGATATCGCATTCACTTACCACTCCTTTCCGCCGGAAGATTTGTTGCGAGCTCTTCCTAATCTGCTCGCATTTCACGTCGATTTAAAATCCGGTCAAGGATTCGATGCCATTGCTGAGAAATTTGGCCAGCCCGATGTTGTTGTTAATTGTGCTGCTATCTCTGTACCTCGTGCCTGCGAAACAGACCCTGCTTCTGCATTGTCGATCAACGTGCCTACTGCCCTTGGAAATTGGATGTTGAGCTTTGAAGAGAGGAATACTCTTCTAGTTCATCTATCAACTGATCAAGTTTATGAAGGCGTGAGATCATTCTACAAGGAAGAAGATGAAACCATTCCTGTGAATGTTTATGGGAAGTCAAAGCTAGCAGCTGAACAGTATGTTTCTGAGAAATTTCCTAACTTTGCAATCTTGAGATCTAGCATCATTTTTGGTCCCCAGACCATCTCACCTGTTCCTAAATCTCTTCCAATTCAGTGGATTGATGCTGTTTTAACCAAAGGAAGCGAAGTTGACTTCTTTCACGATGAGTTTCGATGTCCCGTGTATGTCAAGGACGTTGTAGCTGTCATTATAAAATTGATTAAGACATGGATATCAGAGAGCAAGCAAATGCAGTTGCTGTTAAATGTTGGAGGACCGAATCGTGTATCACGTGTCGAAATGGCCGAGACTGTTGCAGAAATTAGAGGGCACAACAAATCATTGATAAAAAGAGTGTCTGCATCCTCGGTATCCTTGTCTTCCAGAAGATTCGTCTATTATTTCTACTTTTGGTTTGAGTTTCCACTTGAATGGTTTCAGATTGATCGAGGGGTAAAATCCCCTGCTGACATTTCAATGAACATCAGTAAACTGGTTCAGAAACTTGCCATTTCTCCCGTTTCATTCACTCATGGTGTCAGATTGACACTTGCAGCCGAGACT

Protein sequence

MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLKSGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLLVHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTISPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLLNVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQIDRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET
Homology
BLAST of MC07g0384 vs. ExPASy Swiss-Prot
Match: D4GU71 (Probable low-salt glycan biosynthesis reductase Agl14 OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=agl14 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.7e-18
Identity = 59/195 (30.26%), Postives = 99/195 (50.77%), Query Frame = 0

Query: 76  DVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLLVHLSTDQVYEG-VRS 135
           D+V+NCAA +    CE++P  A ++N  TA G+     ++R    +H STD V++G    
Sbjct: 63  DLVINCAAYTDVDGCESNPEVATAVN-GTAPGDLAAVCDDREIPFIHYSTDYVFDGETDG 122

Query: 136 FYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTISPVPKSLPIQWIDA 195
           FY+E DE  P+  YG+SKL  E  V +  P+  ILR S ++G +  +      P QW+ +
Sbjct: 123 FYEEGDEPAPIQEYGRSKLTGEHAVRDVNPDALILRLSFVYGARGDTSDLVGFP-QWVAS 182

Query: 196 VLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLLNVGGPNRVSRVEMA 255
            L  G  V  F D+   P    +V    ++L+   +S +       +V   + V+  +  
Sbjct: 183 TLAAGDTVPLFTDQTMTPSRAGNVATTTLELLDAGVSGT------FHVASQSAVTPSDFG 242

Query: 256 ETVAEIRGHNKSLIK 270
           E + E+ G + +LI+
Sbjct: 243 EKICEVIGGDATLIE 249

BLAST of MC07g0384 vs. ExPASy Swiss-Prot
Match: Q9NZL9 (Methionine adenosyltransferase 2 subunit beta OS=Homo sapiens OX=9606 GN=MAT2B PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.0e-17
Identity = 77/273 (28.21%), Postives = 123/273 (45.05%), Query Frame = 0

Query: 4   KKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLKSGQ 63
           ++VLV G TG LG+ +   F           F           RA P      V+L    
Sbjct: 29  RRVLVTGATGLLGRAVHKEFQQNNWHAVGCGFR----------RARPKF--EQVNLLDSN 88

Query: 64  GFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLLVHL 123
               I   F QP V+V+CAA   P   E  P +A  +NV  A GN           L+++
Sbjct: 89  AVHHIIHDF-QPHVIVHCAAERRPDVVENQPDAASQLNV-DASGNLAKEAAAVGAFLIYI 148

Query: 124 STDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y+EED   P+N+YGK+KL  E+ V E     A+LR  I++G   +  +
Sbjct: 149 SSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYG--EVEKL 208

Query: 184 PKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLLNVG 243
            +S      D V       +  H + R P +VKDV  V  +L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHNKSLIKRVSASSV 277
           G  ++++ EMA  +A+      S ++ ++ S V
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV 284

BLAST of MC07g0384 vs. ExPASy Swiss-Prot
Match: Q5R4E0 (Methionine adenosyltransferase 2 subunit beta OS=Pongo abelii OX=9601 GN=MAT2B PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 3.0e-17
Identity = 77/273 (28.21%), Postives = 123/273 (45.05%), Query Frame = 0

Query: 4   KKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLKSGQ 63
           ++VLV G TG LG+ +   F           F           RA P      V+L    
Sbjct: 29  RRVLVTGATGLLGRAVHKEFQQNNWHAVGCGFR----------RARPKF--EQVNLLDSN 88

Query: 64  GFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLLVHL 123
               I   F QP V+V+CAA   P   E  P +A  +NV  A GN           L+++
Sbjct: 89  AVHHIIHDF-QPHVIVHCAAERRPDVVENQPDAASQLNV-DASGNLAKEAAAVGAFLIYI 148

Query: 124 STDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y+EED   P+N+YGK+KL  E+ V E     A+LR  I++G   +  +
Sbjct: 149 SSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYG--EVEKL 208

Query: 184 PKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLLNVG 243
            +S      D V       +  H + R P +VKDV  V  +L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHNKSLIKRVSASSV 277
           G  ++++ EMA  +A+      S ++ ++ S V
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV 284

BLAST of MC07g0384 vs. ExPASy Swiss-Prot
Match: Q99LB6 (Methionine adenosyltransferase 2 subunit beta OS=Mus musculus OX=10090 GN=Mat2b PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.1e-17
Identity = 74/273 (27.11%), Postives = 125/273 (45.79%), Query Frame = 0

Query: 4   KKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLKSGQ 63
           ++VL+ G TG LG+ +   F           F           RA P      V+L   +
Sbjct: 29  RRVLITGATGLLGRAVYKEFQQSNWHTVGCGFR----------RARPKF--EQVNLLDSE 88

Query: 64  GFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLLVHL 123
               +   F QP V+V+CAA   P   E+ P +A  +NV  A GN           L+++
Sbjct: 89  AVHHLIHDF-QPHVIVHCAAERRPDVVESQPDAASQLNV-GASGNLAKEAAAIGAFLIYI 148

Query: 124 STDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y EED   P+N+YGK+KL  E+ V E     A+LR  +++G   +  +
Sbjct: 149 SSDYVFDGTNPPYTEEDIPSPLNLYGKTKLDGEKAVLENNLGAAVLRIPVLYG--EVEKL 208

Query: 184 PKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLLNVG 243
            +S      D V       +  H + R P +VKDV +V  +L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVASVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHNKSLIKRVSASSV 277
           G  ++++ EMA  +A+      S ++ ++ S V
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV 284

BLAST of MC07g0384 vs. ExPASy Swiss-Prot
Match: Q5U2R0 (Methionine adenosyltransferase 2 subunit beta OS=Rattus norvegicus OX=10116 GN=Mat2b PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 5.1e-17
Identity = 74/273 (27.11%), Postives = 125/273 (45.79%), Query Frame = 0

Query: 4   KKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLKSGQ 63
           ++VL+ G TG LG+ +   F           F           RA P      V+L   +
Sbjct: 29  RRVLITGATGLLGRAVYKEFQQSNWHAVGCGFR----------RARPKF--EQVNLLDSE 88

Query: 64  GFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLLVHL 123
               +   F QP V+V+CAA   P   E+ P +A  +NV  A GN           L+++
Sbjct: 89  AVHHLIHDF-QPHVIVHCAAERRPDVVESQPDAASQLNV-GASGNLAKEAAAIGAFLIYI 148

Query: 124 STDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTISPV 183
           S+D V++G    Y EED   P+N+YGK+KL  E+ V E     A+LR  +++G   +  +
Sbjct: 149 SSDYVFDGTNPPYTEEDIPSPLNLYGKTKLDGEKAVLENNLGAAVLRIPVLYG--EVEKL 208

Query: 184 PKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLLNVG 243
            +S      D V       +  H + R P +VKDV +V  +L +  + +   ++   +  
Sbjct: 209 EESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVASVCRQLAEKRMLD-PSIKGTFHWS 268

Query: 244 GPNRVSRVEMAETVAEIRGHNKSLIKRVSASSV 277
           G  ++++ EMA  +A+      S ++ ++ S V
Sbjct: 269 GNEQMTKYEMACAIADAFNLPSSHLRPITDSPV 284

BLAST of MC07g0384 vs. NCBI nr
Match: XP_022156157.1 (methionine adenosyltransferase 2 subunit beta [Momordica charantia])

HSP 1 Score: 610 bits (1574), Expect = 1.63e-219
Identity = 316/340 (92.94%), Postives = 316/340 (92.94%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK
Sbjct: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL
Sbjct: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI
Sbjct: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL
Sbjct: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASS                        I
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET
Sbjct: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 316

BLAST of MC07g0384 vs. NCBI nr
Match: XP_022999832.1 (uncharacterized protein LOC111494187 [Cucurbita maxima])

HSP 1 Score: 554 bits (1427), Expect = 4.06e-197
Identity = 280/340 (82.35%), Postives = 302/340 (88.82%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MS+KKVLV+GGTGYLGQHLLSGFS I+G PYD+AFTYHSF P+DL+RALP+LLAF VDLK
Sbjct: 1   MSKKKVLVLGGTGYLGQHLLSGFSDIDGVPYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFDAI +KFGQPD+VVNCAA+SVPRACETDPASALSINVPTALGNW+LSFE+ NTLL
Sbjct: 61  SGQGFDAIVQKFGQPDIVVNCAAVSVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEG+RSFYKE+DETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMRSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPV KSLP+QWIDA L+KG+EV+FFHDEFRCPVYVKDVV+VII LIKTWISE KQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGNEVEFFHDEFRCPVYVKDVVSVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNR+SRVEMAETVAEIRGHNKSLIKRVSASS                        I
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNIS LVQKLAISPVSF  GVRLTLAAET
Sbjct: 301 DRGVKSPADISMNISNLVQKLAISPVSFNEGVRLTLAAET 316

BLAST of MC07g0384 vs. NCBI nr
Match: KAG7026017.1 (agl14 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 551 bits (1420), Expect = 4.73e-196
Identity = 280/340 (82.35%), Postives = 301/340 (88.53%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MS+KKVLV+GGTGYLGQHLLSGFS I+G  YD+AFTYHSF P+DL+RALP+LLAF VDLK
Sbjct: 1   MSKKKVLVLGGTGYLGQHLLSGFSDIDGVSYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFDAI +KFGQPD+VVNCAAISVPRACETDPASALSINVPTALGNW+LSFE+ NTLL
Sbjct: 61  SGQGFDAIVQKFGQPDIVVNCAAISVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEG+RSFYKE+DETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMRSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPV KSLP+QWIDA L+KG+EV+FFHDEFRCPVYVKDVV+VII LIKTWISE KQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGNEVEFFHDEFRCPVYVKDVVSVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNR+SRVEMAETVAEIRGHNKSLIKRVSASS                        I
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNIS LVQKLAISPVSF  GVRLTLAAET
Sbjct: 301 DRGVKSPADISMNISNLVQKLAISPVSFNEGVRLTLAAET 316

BLAST of MC07g0384 vs. NCBI nr
Match: XP_023513753.1 (uncharacterized protein LOC111778267 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 551 bits (1419), Expect = 6.71e-196
Identity = 280/340 (82.35%), Postives = 300/340 (88.24%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MS+ KVLV+GGTGYLGQHLLSGFS I+G PYD+AFTYHSF P+DL+RALP+LLAF VDLK
Sbjct: 1   MSKNKVLVLGGTGYLGQHLLSGFSDIDGVPYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFDAI +KFGQPD+VVNCAAISVPRACETDPASALSINVPTALGNW+LSFE+ NTLL
Sbjct: 61  SGQGFDAILQKFGQPDIVVNCAAISVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEG++SFYKE+DETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMKSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPV KSLP+QWIDA L+KGSEV+FFHDEFRCPVYVKDVV VII LIKTWISE KQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNR+SRVEMAETVAEIRGHNKSLIKRVSASS                        I
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNIS LVQKLAISPVSF  GVRLTLAAET
Sbjct: 301 DRGVKSPADISMNISNLVQKLAISPVSFNEGVRLTLAAET 316

BLAST of MC07g0384 vs. NCBI nr
Match: XP_038878319.1 (methionine adenosyltransferase 2 subunit beta [Benincasa hispida])

HSP 1 Score: 550 bits (1416), Expect = 1.92e-195
Identity = 284/340 (83.53%), Postives = 294/340 (86.47%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MS+ KVLVVGGTGYLGQHLLSGF   +G PYDIAFTYHSF P DLLRALP+ LAF VDLK
Sbjct: 1   MSKNKVLVVGGTGYLGQHLLSGFFESDGVPYDIAFTYHSFAPGDLLRALPHFLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFD IAEKFGQPD+VVNCAA+SVPRACETDP SALSINVPTALGNW+LSFE RNTLL
Sbjct: 61  SGQGFDTIAEKFGQPDIVVNCAAVSVPRACETDPVSALSINVPTALGNWLLSFEGRNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEGV SFYKEEDETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVTSFYKEEDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPVPKSLP+QWIDA L+KGSEV+FFHDEFRCPVYVKDVV VII LIKTWISE KQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSKGSEVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNRVSRVEMAETVAEIRGH KSLIKRVSASS                        I
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNISKLVQ LAISPVSFT GVRLTLA ET
Sbjct: 301 DRGVKSPADISMNISKLVQILAISPVSFTDGVRLTLATET 316

BLAST of MC07g0384 vs. ExPASy TrEMBL
Match: A0A6J1DR99 (methionine adenosyltransferase 2 subunit beta OS=Momordica charantia OX=3673 GN=LOC111023110 PE=4 SV=1)

HSP 1 Score: 610 bits (1574), Expect = 7.87e-220
Identity = 316/340 (92.94%), Postives = 316/340 (92.94%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK
Sbjct: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL
Sbjct: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI
Sbjct: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL
Sbjct: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASS                        I
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET
Sbjct: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 316

BLAST of MC07g0384 vs. ExPASy TrEMBL
Match: A0A6J1KBW1 (uncharacterized protein LOC111494187 OS=Cucurbita maxima OX=3661 GN=LOC111494187 PE=4 SV=1)

HSP 1 Score: 554 bits (1427), Expect = 1.97e-197
Identity = 280/340 (82.35%), Postives = 302/340 (88.82%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MS+KKVLV+GGTGYLGQHLLSGFS I+G PYD+AFTYHSF P+DL+RALP+LLAF VDLK
Sbjct: 1   MSKKKVLVLGGTGYLGQHLLSGFSDIDGVPYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFDAI +KFGQPD+VVNCAA+SVPRACETDPASALSINVPTALGNW+LSFE+ NTLL
Sbjct: 61  SGQGFDAIVQKFGQPDIVVNCAAVSVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEG+RSFYKE+DETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMRSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPV KSLP+QWIDA L+KG+EV+FFHDEFRCPVYVKDVV+VII LIKTWISE KQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGNEVEFFHDEFRCPVYVKDVVSVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNR+SRVEMAETVAEIRGHNKSLIKRVSASS                        I
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNIS LVQKLAISPVSF  GVRLTLAAET
Sbjct: 301 DRGVKSPADISMNISNLVQKLAISPVSFNEGVRLTLAAET 316

BLAST of MC07g0384 vs. ExPASy TrEMBL
Match: A0A6J1HJM9 (uncharacterized protein LOC111464173 OS=Cucurbita moschata OX=3662 GN=LOC111464173 PE=4 SV=1)

HSP 1 Score: 550 bits (1416), Expect = 9.31e-196
Identity = 279/340 (82.06%), Postives = 301/340 (88.53%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           MS+KKVLV+GGTGYLGQHLLSGFS I+G  YD+AFTYHSF P+DL+RALP+LLAF VDLK
Sbjct: 1   MSKKKVLVLGGTGYLGQHLLSGFSDIDGVSYDLAFTYHSFAPDDLVRALPHLLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFDAI +KFGQPD+VVNCAAISVPRACETDPASALSINVPTALGNW+LSFE+ NTLL
Sbjct: 61  SGQGFDAIVQKFGQPDIVVNCAAISVPRACETDPASALSINVPTALGNWLLSFEQMNTLL 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEG+RSFYKE+DETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGMRSFYKEDDETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPV KSLP+QWIDA L+KG+EV+FFHDEFRCPVYVKDVV+VII LIKTWISE KQMQL+L
Sbjct: 181 SPVHKSLPVQWIDAALSKGNEVEFFHDEFRCPVYVKDVVSVIITLIKTWISEGKQMQLVL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNR+SRVEMAETVAEIRGHNKSLIKRVSASS                        I
Sbjct: 241 NVGGPNRLSRVEMAETVAEIRGHNKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 340
           DRGVKSPADISMNIS LV+KLAISPVSF  GVRLTLAAET
Sbjct: 301 DRGVKSPADISMNISNLVRKLAISPVSFNEGVRLTLAAET 316

BLAST of MC07g0384 vs. ExPASy TrEMBL
Match: A0A5D3DLR1 (Putative dTDP-4-dehydrorhamnose reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00020 PE=4 SV=1)

HSP 1 Score: 532 bits (1370), Expect = 9.44e-189
Identity = 273/339 (80.53%), Postives = 288/339 (84.96%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           M +KKVLVVGGTGYLGQHLLSG S I+G PYDIAFTYHSF PEDLL ALP+LLAF VDLK
Sbjct: 1   MRKKKVLVVGGTGYLGQHLLSGLSEIDGVPYDIAFTYHSFAPEDLLGALPHLLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFD +AE FGQPD+VVNCAAISVPRACE DP SA S+NVPTALG W+LSFE RNTL 
Sbjct: 61  SGQGFDTVAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEGV+SFY EE+ETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVKSFYNEENETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPVPKSLP+QWIDA L++G EV+FFHDEFRCPVYVKDVV VII LIKTWISE KQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSEGREVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNRVSRVEMAETVAEIRGH KSLIKRVSASS                        I
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAE 339
           DRGVKSPADISMNI KLVQ LA+ PVSFT GVRLTLAAE
Sbjct: 301 DRGVKSPADISMNIDKLVQILAMFPVSFTDGVRLTLAAE 315

BLAST of MC07g0384 vs. ExPASy TrEMBL
Match: A0A1S3CD69 (probable dTDP-4-dehydrorhamnose reductase OS=Cucumis melo OX=3656 GN=LOC103499064 PE=4 SV=1)

HSP 1 Score: 532 bits (1370), Expect = 9.44e-189
Identity = 273/339 (80.53%), Postives = 288/339 (84.96%), Query Frame = 0

Query: 1   MSRKKVLVVGGTGYLGQHLLSGFSGIEGAPYDIAFTYHSFPPEDLLRALPNLLAFHVDLK 60
           M +KKVLVVGGTGYLGQHLLSG S I+G PYDIAFTYHSF PEDLL ALP+LLAF VDLK
Sbjct: 1   MRKKKVLVVGGTGYLGQHLLSGLSEIDGVPYDIAFTYHSFAPEDLLGALPHLLAFQVDLK 60

Query: 61  SGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNTLL 120
           SGQGFD +AE FGQPD+VVNCAAISVPRACE DP SA S+NVPTALG W+LSFE RNTL 
Sbjct: 61  SGQGFDTVAENFGQPDIVVNCAAISVPRACEIDPVSAFSVNVPTALGTWLLSFEGRNTLF 120

Query: 121 VHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQTI 180
           +HLSTDQVYEGV+SFY EE+ETIPVNVYGKSKLAAEQYVSEKF NFAILRSSIIFGPQTI
Sbjct: 121 IHLSTDQVYEGVKSFYNEENETIPVNVYGKSKLAAEQYVSEKFSNFAILRSSIIFGPQTI 180

Query: 181 SPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQLLL 240
           SPVPKSLP+QWIDA L++G EV+FFHDEFRCPVYVKDVV VII LIKTWISE KQMQLLL
Sbjct: 181 SPVPKSLPVQWIDATLSEGREVEFFHDEFRCPVYVKDVVNVIITLIKTWISEGKQMQLLL 240

Query: 241 NVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWFQI 300
           NVGGPNRVSRVEMAETVAEIRGH KSLIKRVSASS                        I
Sbjct: 241 NVGGPNRVSRVEMAETVAEIRGHKKSLIKRVSASS------------------------I 300

Query: 301 DRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAE 339
           DRGVKSPADISMNI KLVQ LA+ PVSFT GVRLTLAAE
Sbjct: 301 DRGVKSPADISMNIDKLVQILAMFPVSFTDGVRLTLAAE 315

BLAST of MC07g0384 vs. TAIR 10
Match: AT4G00560.4 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 421.0 bits (1081), Expect = 9.0e-118
Identity = 215/342 (62.87%), Postives = 262/342 (76.61%), Query Frame = 0

Query: 3   RKKVLVVGGTGYLGQHLLSGFSGIEGAP---YDIAFTYHSFP-PEDLLRALPNLLAFHVD 62
           + KVL+VGGTGYLGQHLL  F+G  G     YD+AFT+HS P P  LL A P+  AF VD
Sbjct: 4   KTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPVD 63

Query: 63  LKSGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNT 122
           LKSG G ++I++ F QPDVVVNCAA+SVPRACE DP SA+SINVPT+L NW+ SFE   T
Sbjct: 64  LKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNKT 123

Query: 123 LLVHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQ 182
           LL+HLSTDQVY+GV+SFYKEEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGPQ
Sbjct: 124 LLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGPQ 183

Query: 183 TISPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQL 242
           T+SP+PK+LPIQWID+ L KG  VDFFHDEFRCP+YVKD+V +  KLI  W+S+ KQM+L
Sbjct: 184 TVSPLPKTLPIQWIDSSLKKGDTVDFFHDEFRCPIYVKDLVNITFKLIDRWVSDDKQMRL 243

Query: 243 LLNVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWF 302
           +LN GGP R+SRV+MA+ VAE+RG++ SLIK VSASS+                    + 
Sbjct: 244 VLNAGGPERLSRVQMAQMVAEVRGYDLSLIKHVSASSL--------------------FC 303

Query: 303 QIDRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 341
           QIDRGV SPADISM+I+KL+  L +SP SF  GVRLTL +E+
Sbjct: 304 QIDRGVVSPADISMDITKLIHTLELSPTSFKEGVRLTLDSES 325

BLAST of MC07g0384 vs. TAIR 10
Match: AT4G00560.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 5.9e-117
Identity = 214/342 (62.57%), Postives = 259/342 (75.73%), Query Frame = 0

Query: 3   RKKVLVVGGTGYLGQHLLSGFSGIEGAP---YDIAFTYHSFP-PEDLLRALPNLLAFHVD 62
           + KVL+VGGTGYLGQHLL  F+G  G     YD+AFT+HS P P  LL A P+  AF VD
Sbjct: 4   KTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPVD 63

Query: 63  LKSGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNT 122
           LKSG G ++I++ F QPDVVVNCAA+SVPRACE DP SA+SINVPT+L NW+ SFE   T
Sbjct: 64  LKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNKT 123

Query: 123 LLVHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQ 182
           LL+HLSTDQVY+GV+SFYKEEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGPQ
Sbjct: 124 LLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGPQ 183

Query: 183 TISPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWISESKQMQL 242
           T+SP+PK+LPIQWID+ L KG  VDFFHDEFRCP+YVKD+V +  KLI  W+S+ KQM+L
Sbjct: 184 TVSPLPKTLPIQWIDSSLKKGDTVDFFHDEFRCPIYVKDLVNITFKLIDRWVSDDKQMRL 243

Query: 243 LLNVGGPNRVSRVEMAETVAEIRGHNKSLIKRVSASSVSLSSRRFVYYFYFWFEFPLEWF 302
           +LN GGP R+SRV+MA+ VAE+RG++ SLIK VSASS                       
Sbjct: 244 VLNAGGPERLSRVQMAQMVAEVRGYDLSLIKHVSASS----------------------- 303

Query: 303 QIDRGVKSPADISMNISKLVQKLAISPVSFTHGVRLTLAAET 341
            IDRGV SPADISM+I+KL+  L +SP SF  GVRLTL +E+
Sbjct: 304 -IDRGVVSPADISMDITKLIHTLELSPTSFKEGVRLTLDSES 321

BLAST of MC07g0384 vs. TAIR 10
Match: AT4G00560.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 324.3 bits (830), Expect = 1.2e-88
Identity = 158/233 (67.81%), Postives = 188/233 (80.69%), Query Frame = 0

Query: 3   RKKVLVVGGTGYLGQHLLSGFSGIEGAP---YDIAFTYHSFP-PEDLLRALPNLLAFHVD 62
           + KVL+VGGTGYLGQHLL  F+G  G     YD+AFT+HS P P  LL A P+  AF VD
Sbjct: 4   KTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPVD 63

Query: 63  LKSGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNT 122
           LKSG G ++I++ F QPDVVVNCAA+SVPRACE DP SA+SINVPT+L NW+ SFE   T
Sbjct: 64  LKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNKT 123

Query: 123 LLVHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQ 182
           LL+HLSTDQVY+GV+SFYKEEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGPQ
Sbjct: 124 LLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGPQ 183

Query: 183 TISPVPKSLPIQWIDAVLTKGSEVDFFHDEFRCPVYVKDVVAVIIKLIKTWIS 232
           T+SP+PK+LPIQWID+ L KG  VDFFHDEFRCP+YVKD+V +  KLI  W+S
Sbjct: 184 TVSPLPKTLPIQWIDSSLKKGDTVDFFHDEFRCPIYVKDLVNITFKLIDRWVS 236

BLAST of MC07g0384 vs. TAIR 10
Match: AT4G00560.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.8e-70
Identity = 132/195 (67.69%), Postives = 158/195 (81.03%), Query Frame = 0

Query: 3   RKKVLVVGGTGYLGQHLLSGFSGIEGAP---YDIAFTYHSFP-PEDLLRALPNLLAFHVD 62
           + KVL+VGGTGYLGQHLL  F+G  G     YD+AFT+HS P P  LL A P+  AF VD
Sbjct: 4   KTKVLIVGGTGYLGQHLLQAFAGNYGGECELYDVAFTHHSSPLPARLLDAFPHSPAFPVD 63

Query: 63  LKSGQGFDAIAEKFGQPDVVVNCAAISVPRACETDPASALSINVPTALGNWMLSFEERNT 122
           LKSG G ++I++ F QPDVVVNCAA+SVPRACE DP SA+SINVPT+L NW+ SFE   T
Sbjct: 64  LKSGLGLNSISQDFRQPDVVVNCAALSVPRACEQDPDSAMSINVPTSLVNWLSSFETNKT 123

Query: 123 LLVHLSTDQVYEGVRSFYKEEDETIPVNVYGKSKLAAEQYVSEKFPNFAILRSSIIFGPQ 182
           LL+HLSTDQVY+GV+SFYKEEDET+ VNVYGKSK+AAE  + +K  +FAILRSSIIFGPQ
Sbjct: 124 LLIHLSTDQVYQGVKSFYKEEDETVAVNVYGKSKVAAELLIKDKCQSFAILRSSIIFGPQ 183

Query: 183 TISPVPKSLPIQWID 194
           T+SP+PK+LPIQ I+
Sbjct: 184 TVSPLPKTLPIQMIN 198

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
D4GU712.7e-1830.26Probable low-salt glycan biosynthesis reductase Agl14 OS=Haloferax volcanii (str... [more]
Q9NZL93.0e-1728.21Methionine adenosyltransferase 2 subunit beta OS=Homo sapiens OX=9606 GN=MAT2B P... [more]
Q5R4E03.0e-1728.21Methionine adenosyltransferase 2 subunit beta OS=Pongo abelii OX=9601 GN=MAT2B P... [more]
Q99LB65.1e-1727.11Methionine adenosyltransferase 2 subunit beta OS=Mus musculus OX=10090 GN=Mat2b ... [more]
Q5U2R05.1e-1727.11Methionine adenosyltransferase 2 subunit beta OS=Rattus norvegicus OX=10116 GN=M... [more]
Match NameE-valueIdentityDescription
XP_022156157.11.63e-21992.94methionine adenosyltransferase 2 subunit beta [Momordica charantia][more]
XP_022999832.14.06e-19782.35uncharacterized protein LOC111494187 [Cucurbita maxima][more]
KAG7026017.14.73e-19682.35agl14 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023513753.16.71e-19682.35uncharacterized protein LOC111778267 [Cucurbita pepo subsp. pepo][more]
XP_038878319.11.92e-19583.53methionine adenosyltransferase 2 subunit beta [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DR997.87e-22092.94methionine adenosyltransferase 2 subunit beta OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1KBW11.97e-19782.35uncharacterized protein LOC111494187 OS=Cucurbita maxima OX=3661 GN=LOC111494187... [more]
A0A6J1HJM99.31e-19682.06uncharacterized protein LOC111464173 OS=Cucurbita moschata OX=3662 GN=LOC1114641... [more]
A0A5D3DLR19.44e-18980.53Putative dTDP-4-dehydrorhamnose reductase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CD699.44e-18980.53probable dTDP-4-dehydrorhamnose reductase OS=Cucumis melo OX=3656 GN=LOC10349906... [more]
Match NameE-valueIdentityDescription
AT4G00560.49.0e-11862.87NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G00560.15.9e-11762.57NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G00560.31.2e-8867.81NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G00560.21.8e-7067.69NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029903RmlD-like substrate binding domainPFAMPF04321RmlD_sub_bindcoord: 5..282
e-value: 9.1E-38
score: 130.0
NoneNo IPR availableGENE3D3.40.50.720coord: 3..286
e-value: 3.1E-57
score: 196.4
NoneNo IPR availablePANTHERPTHR43242NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 3..339
NoneNo IPR availableCDDcd05254dTDP_HR_like_SDR_ecoord: 5..325
e-value: 1.44419E-69
score: 216.724
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 5..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0384.1MC07g0384.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016740 transferase activity