MC07g0353 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0353
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	U-box domain-containing protein kinase family protein
Location	MC07: 11392352 .. 11392783 (-)
RNA-Seq Expression	MC07g0353
Synteny	MC07g0353

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCTTGAATAAGTTGAGACATCCAAATATTGCGACGCTCATTGGAGCTTGCTCGGAGTCTTGCATTCTTGTTTATGACTATTTAGCCAATGGAAGCCTAGAAGATAGGCTAGCCTGCAAGGACAATTCTACCCCATTATCATGGAAAACTCGAATATGTATTGCCACACAATTATGCTCGGCCCTTATATTTCTTCATACAAACAAACTTTGCAGGGTTATTCATCGTGACTTGAAGCCTACAAATATACTATTAGATGCCAACTATGTTCCTAAACTTTCAGGATTTGGCAAATGCCATTTTCTATCTCACGATGAAACTACATCTGTTGGAGATGACATAGAGGGCAACCGTGAGTTTCCGCTCACAACAAAGTTGGACGTTTTCTCATTTGGCGTAGTATTGTTAAGGTTGTTAACAGGAAAATCAGAC

mRNA sequence

GTCTTGAATAAGTTGAGACATCCAAATATTGCGACGCTCATTGGAGCTTGCTCGGAGTCTTGCATTCTTGTTTATGACTATTTAGCCAATGGAAGCCTAGAAGATAGGCTAGCCTGCAAGGACAATTCTACCCCATTATCATGGAAAACTCGAATATGTATTGCCACACAATTATGCTCGGCCCTTATATTTCTTCATACAAACAAACTTTGCAGGGTTATTCATCGTGACTTGAAGCCTACAAATATACTATTAGATGCCAACTATGTTCCTAAACTTTCAGGATTTGGCAAATGCCATTTTCTATCTCACGATGAAACTACATCTGTTGGAGATGACATAGAGGGCAACCGTGAGTTTCCGCTCACAACAAAGTTGGACGTTTTCTCATTTGGCGTAGTATTGTTAAGGTTGTTAACAGGAAAATCAGAC

Coding sequence (CDS)

GTCTTGAATAAGTTGAGACATCCAAATATTGCGACGCTCATTGGAGCTTGCTCGGAGTCTTGCATTCTTGTTTATGACTATTTAGCCAATGGAAGCCTAGAAGATAGGCTAGCCTGCAAGGACAATTCTACCCCATTATCATGGAAAACTCGAATATGTATTGCCACACAATTATGCTCGGCCCTTATATTTCTTCATACAAACAAACTTTGCAGGGTTATTCATCGTGACTTGAAGCCTACAAATATACTATTAGATGCCAACTATGTTCCTAAACTTTCAGGATTTGGCAAATGCCATTTTCTATCTCACGATGAAACTACATCTGTTGGAGATGACATAGAGGGCAACCGTGAGTTTCCGCTCACAACAAAGTTGGACGTTTTCTCATTTGGCGTAGTATTGTTAAGGTTGTTAACAGGAAAATCAGAC

Protein sequence

VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCSALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDIEGNREFPLTTKLDVFSFGVVLLRLLTGKSD

Homology

[Close]

BLAST of MC07g0353 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 175.3 bits (443), Expect = 5.1e-43
Identity = 86/151 (56.95%), Postives = 107/151 (70.86%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+K+RHPNI TLIGAC E   LVY+YL  GSLEDRL CKDNS PLSW+ R+ IAT++C+
Sbjct: 527 VLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEICA 586

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDIEGNREF 120
           AL+FLH+NK   ++H DLKP NILLD+N V KLS FG C  L  + + SV  D+ G   +
Sbjct: 587 ALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAY 646

Query: 121 ---------PLTTKLDVFSFGVVLLRLLTGK 143
                     LT K DV+SFG++LLRLLTG+
Sbjct: 647 LDPEASSSGELTPKSDVYSFGIILLRLLTGR 677

[Close]

BLAST of MC07g0353 vs. ExPASy Swiss-Prot
Match: Q5WA76 (U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB70 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 2.6e-34
Identity = 78/155 (50.32%), Postives = 99/155 (63.87%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           +L+++RHP++ TL+GACSES  LVY++L NGSLED L C D    L+W+ RI I  ++CS
Sbjct: 491 ILSRVRHPHLVTLLGACSESSTLVYEFLPNGSLEDFLMCSDKRQTLTWQARIRIIAEICS 550

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTS---------VG 120
           ALIFLH NK   V+H DLKP NILL  N V KLS FG    L    T +         VG
Sbjct: 551 ALIFLHKNKPHPVVHGDLKPANILLGVNLVSKLSDFGISRLLIQSSTNNTTLYRTMHPVG 610

Query: 121 DDIEGNREF----PLTTKLDVFSFGVVLLRLLTGK 143
             +  + EF     LT + DV+SFG+V+LRLLTGK
Sbjct: 611 TPLYMDPEFLSTGELTPQSDVYSFGIVVLRLLTGK 645

[Close]

BLAST of MC07g0353 vs. ExPASy Swiss-Prot
Match: Q0DR28 (U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB57 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 7.0e-32
Identity = 71/148 (47.97%), Postives = 91/148 (61.49%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           +L + RHPNI T IG CSE   LVY++L NG+LEDR+ C +NS PLSW  R  I  ++C 
Sbjct: 200 ILRQWRHPNIITFIGVCSEVSALVYEWLPNGNLEDRIICTNNSAPLSWYNRTQIIGEICC 259

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVG-------DD 120
           AL+FLH+NK   ++H DL+P NIL+DANY  K+  FG  +      T            D
Sbjct: 260 ALLFLHSNKSTALVHGDLRPCNILIDANYRSKICNFGMSNLFLQLGTFPPNLTARLPYMD 319

Query: 121 IEGNREFPLTTKLDVFSFGVVLLRLLTG 142
            E N    LTT  DV+S GV++LRLLTG
Sbjct: 320 PEFNTTGELTTLSDVYSLGVIILRLLTG 347

[Close]

BLAST of MC07g0353 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 132.1 bits (331), Expect = 5.0e-30
Identity = 70/156 (44.87%), Postives = 98/156 (62.82%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           +L+K+RHP++  L+GAC E   LVY+Y+ NGSLEDRL   +NS PL W  R  IA ++ +
Sbjct: 526 ILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAA 585

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFG----------KCHFLSHDETTSV 120
           AL+FLH +K   +IHRDLKP NILLD N+V K+   G             F  + +T+ V
Sbjct: 586 ALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPV 645

Query: 121 GD----DIEGNREFPLTTKLDVFSFGVVLLRLLTGK 143
           G     D E  R   +++K D++SFG++LL+LLT K
Sbjct: 646 GTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAK 681

[Close]

BLAST of MC07g0353 vs. ExPASy Swiss-Prot
Match: Q94A51 (U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 9.4e-29
Identity = 63/144 (43.75%), Postives = 100/144 (69.44%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           +L+++RHPN+ TL+GAC ES  L+Y Y+ NGSLED  + ++N   LSW++RI IA+++CS
Sbjct: 506 ILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESRIRIASEICS 565

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDIEG---- 120
           AL+FLH+N  C +IH +LKP+ ILLD+N V K++ +G    +  D        ++     
Sbjct: 566 ALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHYFV 625

Query: 121 NREFPLTTKLDVFSFGVVLLRLLT 141
           +RE  +T + D+++FG++LL+LLT
Sbjct: 626 SRE--MTLESDIYAFGIILLQLLT 646

[Close]

BLAST of MC07g0353 vs. NCBI nr
Match: XP_022157677.1 (U-box domain-containing protein 33-like [Momordica charantia])

HSP 1 Score: 256 bits (655), Expect = 6.37e-78
Identity = 124/144 (86.11%), Postives = 130/144 (90.28%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VLNKLRHPNI TLIGACSESCILVYDY ANGSLEDRLACKDNS+PLSWKTRI IATQLCS
Sbjct: 521 VLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQLCS 580

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDIEGNREF 120
           AL+FLHTNKLCRVIH DL P NILLD NY+PKL+GFG CHFL +DETTSV  DIE N E 
Sbjct: 581 ALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNHES 640

Query: 121 PLTTKLDVFSFGVVLLRLLTGKSD 144
           PLTTKLDVFSFG+VLLRLLTGKSD
Sbjct: 641 PLTTKLDVFSFGIVLLRLLTGKSD 664

[Close]

BLAST of MC07g0353 vs. NCBI nr
Match: XP_038907096.1 (U-box domain-containing protein 33-like isoform X2 [Benincasa hispida])

HSP 1 Score: 233 bits (593), Expect = 8.53e-70
Identity = 108/149 (72.48%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+KLRHPNIATLIG C E+CILVYDY  NG+LEDRLACKDNS+PLSWKTRI IAT+LCS
Sbjct: 386 VLSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRIRIATELCS 445

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDI------ 120
           ALIF+H+NK+C++IH D+KP+N+LLDANYVPKL+GFG CHFL HDE +S  +D+      
Sbjct: 446 ALIFIHSNKVCKIIHGDMKPSNVLLDANYVPKLAGFGTCHFLPHDEKSSYNEDLSAGYDA 505

Query: 121 EGNREFPLTTKLDVFSFGVVLLRLLTGKS 143
           +GN EFPLTT+LDVFSFG++LL LLTG+S
Sbjct: 506 KGNHEFPLTTELDVFSFGMILLSLLTGQS 534

[Close]

BLAST of MC07g0353 vs. NCBI nr
Match: XP_038907095.1 (U-box domain-containing protein 33-like isoform X1 [Benincasa hispida])

HSP 1 Score: 233 bits (593), Expect = 5.46e-69
Identity = 108/149 (72.48%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+KLRHPNIATLIG C E+CILVYDY  NG+LEDRLACKDNS+PLSWKTRI IAT+LCS
Sbjct: 508 VLSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRIRIATELCS 567

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDI------ 120
           ALIF+H+NK+C++IH D+KP+N+LLDANYVPKL+GFG CHFL HDE +S  +D+      
Sbjct: 568 ALIFIHSNKVCKIIHGDMKPSNVLLDANYVPKLAGFGTCHFLPHDEKSSYNEDLSAGYDA 627

Query: 121 EGNREFPLTTKLDVFSFGVVLLRLLTGKS 143
           +GN EFPLTT+LDVFSFG++LL LLTG+S
Sbjct: 628 KGNHEFPLTTELDVFSFGMILLSLLTGQS 656

[Close]

BLAST of MC07g0353 vs. NCBI nr
Match: XP_023547656.1 (U-box domain-containing protein 33-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 229 bits (583), Expect = 2.48e-68
Identity = 109/149 (73.15%), Postives = 126/149 (84.56%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+ LRHPNIATLIG C E+C+LVYDY  NGSLEDRLACKDNS+PLSWKTRI IA++LCS
Sbjct: 388 VLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCS 447

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDI------ 120
           ALIF+H+NK+C++IH DLKP N+LLDANYVPKL+GFG CHFL HDE  S  ++I      
Sbjct: 448 ALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPAKYGV 507

Query: 121 EGNREFPLTTKLDVFSFGVVLLRLLTGKS 143
           + N EFPLTTKLDVFSFG+VLL LLTGKS
Sbjct: 508 KSNHEFPLTTKLDVFSFGMVLLSLLTGKS 536

[Close]

BLAST of MC07g0353 vs. NCBI nr
Match: XP_031743838.1 (U-box domain-containing protein 33 [Cucumis sativus] >KGN45661.1 hypothetical protein Csa_004782 [Cucumis sativus])

HSP 1 Score: 231 bits (588), Expect = 2.80e-68
Identity = 109/148 (73.65%), Postives = 126/148 (85.14%), Query Frame = 0


Query: 2   LNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCSA 61
           L+KLRHPNIATLIG C E+CILVYDY  NG+LEDRLACKDNS+PLSWKTRI IATQLCSA
Sbjct: 509 LSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATQLCSA 568

Query: 62  LIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDE------TTSVGDDIE 121
           LIF+H+NK+C+ +H D+KP+NILLDANYVPKL+GFG CHFL+HDE        SVG D +
Sbjct: 569 LIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFGTCHFLTHDEKLSYNENLSVGYDAK 628

Query: 122 GNREFPLTTKLDVFSFGVVLLRLLTGKS 143
            N EFPLTT+LD++SFG VLL LLTGKS
Sbjct: 629 RNHEFPLTTELDIYSFGTVLLSLLTGKS 656

[Close]

BLAST of MC07g0353 vs. ExPASy TrEMBL
Match: A0A6J1DV42 (U-box domain-containing protein 33-like OS=Momordica charantia OX=3673 GN=LOC111024334 PE=4 SV=1)

HSP 1 Score: 256 bits (655), Expect = 3.08e-78
Identity = 124/144 (86.11%), Postives = 130/144 (90.28%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VLNKLRHPNI TLIGACSESCILVYDY ANGSLEDRLACKDNS+PLSWKTRI IATQLCS
Sbjct: 521 VLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQLCS 580

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDIEGNREF 120
           AL+FLHTNKLCRVIH DL P NILLD NY+PKL+GFG CHFL +DETTSV  DIE N E 
Sbjct: 581 ALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNHES 640

Query: 121 PLTTKLDVFSFGVVLLRLLTGKSD 144
           PLTTKLDVFSFG+VLLRLLTGKSD
Sbjct: 641 PLTTKLDVFSFGIVLLRLLTGKSD 664

[Close]

BLAST of MC07g0353 vs. ExPASy TrEMBL
Match: A0A0A0KD01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004520 PE=4 SV=1)

HSP 1 Score: 231 bits (588), Expect = 1.35e-68
Identity = 109/148 (73.65%), Postives = 126/148 (85.14%), Query Frame = 0


Query: 2   LNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCSA 61
           L+KLRHPNIATLIG C E+CILVYDY  NG+LEDRLACKDNS+PLSWKTRI IATQLCSA
Sbjct: 509 LSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATQLCSA 568

Query: 62  LIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDE------TTSVGDDIE 121
           LIF+H+NK+C+ +H D+KP+NILLDANYVPKL+GFG CHFL+HDE        SVG D +
Sbjct: 569 LIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFGTCHFLTHDEKLSYNENLSVGYDAK 628

Query: 122 GNREFPLTTKLDVFSFGVVLLRLLTGKS 143
            N EFPLTT+LD++SFG VLL LLTGKS
Sbjct: 629 RNHEFPLTTELDIYSFGTVLLSLLTGKS 656

[Close]

BLAST of MC07g0353 vs. ExPASy TrEMBL
Match: A0A6J1H3L1 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1)

HSP 1 Score: 228 bits (582), Expect = 1.68e-68
Identity = 110/149 (73.83%), Postives = 126/149 (84.56%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+ LRHPNIATLIG C E+CILVYDY  NGSLEDRLACKDNS+PLSWKTRI IA++LCS
Sbjct: 388 VLSILRHPNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCS 447

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDI------ 120
           ALIF+H+NK+C++IH DLKP N+LLDANYVPKL+GFG CHFL HDE  S  ++I      
Sbjct: 448 ALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKYGV 507

Query: 121 EGNREFPLTTKLDVFSFGVVLLRLLTGKS 143
           + N EFPLTTKLDVFSFG+VLL LLTGKS
Sbjct: 508 KSNPEFPLTTKLDVFSFGMVLLSLLTGKS 536

[Close]

BLAST of MC07g0353 vs. ExPASy TrEMBL
Match: A0A6J1H3V9 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1)

HSP 1 Score: 228 bits (582), Expect = 1.01e-67
Identity = 110/149 (73.83%), Postives = 126/149 (84.56%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+ LRHPNIATLIG C E+CILVYDY  NGSLEDRLACKDNS+PLSWKTRI IA++LCS
Sbjct: 510 VLSILRHPNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASELCS 569

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDI------ 120
           ALIF+H+NK+C++IH DLKP N+LLDANYVPKL+GFG CHFL HDE  S  ++I      
Sbjct: 570 ALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKYGV 629

Query: 121 EGNREFPLTTKLDVFSFGVVLLRLLTGKS 143
           + N EFPLTTKLDVFSFG+VLL LLTGKS
Sbjct: 630 KSNPEFPLTTKLDVFSFGMVLLSLLTGKS 658

[Close]

BLAST of MC07g0353 vs. ExPASy TrEMBL
Match: A0A5D3BV84 (E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001100 PE=4 SV=1)

HSP 1 Score: 228 bits (581), Expect = 1.39e-67
Identity = 106/148 (71.62%), Postives = 126/148 (85.14%), Query Frame = 0


Query: 2   LNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCSA 61
           L+KLRHPN+ATLIG C E+CILVYDY  NG+LEDRLACKDNS+PLSWKTRI IAT LCSA
Sbjct: 510 LSKLRHPNLATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATDLCSA 569

Query: 62  LIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTS------VGDDIE 121
           LIF+H+NK+C+++H D+KP+N+LLDANYVPKL+GFG CHFL+HDE +S      VG D  
Sbjct: 570 LIFIHSNKICKIVHGDMKPSNVLLDANYVPKLAGFGICHFLTHDEKSSYNENLSVGHDAM 629

Query: 122 GNREFPLTTKLDVFSFGVVLLRLLTGKS 143
           GN EFPLTT  DV+SFG+VLL LLTG+S
Sbjct: 630 GNHEFPLTTAFDVYSFGMVLLSLLTGQS 657

[Close]

BLAST of MC07g0353 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 175.3 bits (443), Expect = 3.7e-44
Identity = 86/151 (56.95%), Postives = 107/151 (70.86%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+K+RHPNI TLIGAC E   LVY+YL  GSLEDRL CKDNS PLSW+ R+ IAT++C+
Sbjct: 527 VLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEICA 586

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDIEGNREF 120
           AL+FLH+NK   ++H DLKP NILLD+N V KLS FG C  L  + + SV  D+ G   +
Sbjct: 587 ALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTVAY 646

Query: 121 ---------PLTTKLDVFSFGVVLLRLLTGK 143
                     LT K DV+SFG++LLRLLTG+
Sbjct: 647 LDPEASSSGELTPKSDVYSFGIILLRLLTGR 677

[Close]

BLAST of MC07g0353 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 132.1 bits (331), Expect = 3.6e-31
Identity = 70/156 (44.87%), Postives = 98/156 (62.82%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           +L+K+RHP++  L+GAC E   LVY+Y+ NGSLEDRL   +NS PL W  R  IA ++ +
Sbjct: 526 ILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAA 585

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFG----------KCHFLSHDETTSV 120
           AL+FLH +K   +IHRDLKP NILLD N+V K+   G             F  + +T+ V
Sbjct: 586 ALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPV 645

Query: 121 GD----DIEGNREFPLTTKLDVFSFGVVLLRLLTGK 143
           G     D E  R   +++K D++SFG++LL+LLT K
Sbjct: 646 GTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAK 681

[Close]

BLAST of MC07g0353 vs. TAIR 10
Match: AT5G12000.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 131.7 bits (330), Expect = 4.6e-31
Identity = 70/156 (44.87%), Postives = 97/156 (62.18%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+ +RHP++  L+GAC E   LVY+++ NGSLEDRL  + NS PLSW+ R  IA ++ +
Sbjct: 468 VLSSIRHPHMVLLLGACPEYGCLVYEFMDNGSLEDRLFRRGNSPPLSWRKRFQIAAEIAT 527

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFL---------SHDETTSVG 120
           AL FLH  K   ++HRDLKP NILLD NYV K+S  G    +          +  T++ G
Sbjct: 528 ALSFLHQAKPEPLVHRDLKPANILLDKNYVSKISDVGLARLVPASVANTVTQYHMTSAAG 587

Query: 121 D----DIEGNREFPLTTKLDVFSFGVVLLRLLTGKS 144
                D E  +   LTTK D+FS G++LL+++T KS
Sbjct: 588 TFCYIDPEYQQTGKLTTKSDIFSLGIMLLQIITAKS 623

[Close]

BLAST of MC07g0353 vs. TAIR 10
Match: AT1G17540.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 129.0 bits (323), Expect = 3.0e-30
Identity = 67/153 (43.79%), Postives = 95/153 (62.09%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           VL+ +RHPN+  L+GAC E   LVY+Y+ NG+LEDRL CKDN+ PLSW+ R  IA ++ +
Sbjct: 455 VLSCMRHPNMVILLGACPEYGCLVYEYMENGTLEDRLFCKDNTPPLSWRARFRIAAEIAT 514

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCH---------FLSHDETTSVG 120
            L+FLH  K   ++HRDLKP NIL+D ++  K+S  G            F ++  T + G
Sbjct: 515 GLLFLHQAKPEPLVHRDLKPANILIDRHFTSKISDVGLARLVPAAVADSFSNYHMTAAAG 574

Query: 121 D----DIEGNREFPLTTKLDVFSFGVVLLRLLT 141
                D E  +   L  K D++SFGVVLL+++T
Sbjct: 575 TFCYIDPEYQQTGMLGVKSDLYSFGVVLLQIIT 607

[Close]

BLAST of MC07g0353 vs. TAIR 10
Match: AT3G49060.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 127.9 bits (320), Expect = 6.7e-30
Identity = 63/144 (43.75%), Postives = 100/144 (69.44%), Query Frame = 0


Query: 1   VLNKLRHPNIATLIGACSESCILVYDYLANGSLEDRLACKDNSTPLSWKTRICIATQLCS 60
           +L+++RHPN+ TL+GAC ES  L+Y Y+ NGSLED  + ++N   LSW++RI IA+++CS
Sbjct: 506 ILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESRIRIASEICS 565

Query: 61  ALIFLHTNKLCRVIHRDLKPTNILLDANYVPKLSGFGKCHFLSHDETTSVGDDIEG---- 120
           AL+FLH+N  C +IH +LKP+ ILLD+N V K++ +G    +  D        ++     
Sbjct: 566 ALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHYFV 625

Query: 121 NREFPLTTKLDVFSFGVVLLRLLT 141
           +RE  +T + D+++FG++LL+LLT
Sbjct: 626 SRE--MTLESDIYAFGIILLQLLT 646

The following BLAST results are available for this feature:

BLAST of MC07g0353 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GUH1	5.1e-43	56.95	U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2...	[more]
Q5WA76	2.6e-34	50.32	U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=P...	[more]
Q0DR28	7.0e-32	47.97	U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica OX=39947 GN=P...	[more]
Q9SW11	5.0e-30	44.87	U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2...	[more]
Q94A51	9.4e-29	43.75	U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1...	[more]

BLAST of MC07g0353 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157677.1	6.37e-78	86.11	U-box domain-containing protein 33-like [Momordica charantia]	[more]
XP_038907096.1	8.53e-70	72.48	U-box domain-containing protein 33-like isoform X2 [Benincasa hispida]	[more]
XP_038907095.1	5.46e-69	72.48	U-box domain-containing protein 33-like isoform X1 [Benincasa hispida]	[more]
XP_023547656.1	2.48e-68	73.15	U-box domain-containing protein 33-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_031743838.1	2.80e-68	73.65	U-box domain-containing protein 33 [Cucumis sativus] >KGN45661.1 hypothetical pr...	[more]

BLAST of MC07g0353 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DV42	3.08e-78	86.11	U-box domain-containing protein 33-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0KD01	1.35e-68	73.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004520 PE=4 SV=1	[more]
A0A6J1H3L1	1.68e-68	73.83	E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1	[more]
A0A6J1H3V9	1.01e-67	73.83	E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1	[more]
A0A5D3BV84	1.39e-67	71.62	E3 ubiquitin ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G0...	[more]

BLAST of MC07g0353 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45910.1	3.7e-44	56.95	U-box domain-containing protein kinase family protein	[more]
AT4G25160.1	3.6e-31	44.87	U-box domain-containing protein kinase family protein	[more]
AT5G12000.1	4.6e-31	44.87	Protein kinase protein with adenine nucleotide alpha hydrolases-like domain	[more]
AT1G17540.1	3.0e-30	43.79	Protein kinase protein with adenine nucleotide alpha hydrolases-like domain	[more]
AT3G49060.1	6.7e-30	43.75	U-box domain-containing protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 1..144 e-value: 0.0016 score: -86.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 1..142 e-value: 9.3E-23 score: 80.9
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..144 score: 23.354328
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 1..144 e-value: 8.6E-40 score: 138.5
None	No IPR available	PANTHER	PTHR45647	OS02G0152300 PROTEIN	coord: 1..143
None	No IPR available	PANTHER	PTHR45647:SF100	U-BOX DOMAIN-CONTAINING PROTEIN 33	coord: 1..143
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 73..85
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0353.1	MC07g0353.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity