MC07g0352 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0352
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	U-box domain-containing protein kinase family protein
Location	MC07: 11369338 .. 11371861 (-)
RNA-Seq Expression	MC07g0352
Synteny	MC07g0352

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGATGTGTTGAATAAGTTGAGACATCCAAATATTATGACGCTCATTGGAGCTTGCTCGGAGTCTTGCATTCTTGTTTATGACTATTTCGCCAATGGAAGCCTAGAAGATAGGCTAGCCTGCAAGGACAATTCTTCCCCATTATCATGGAAAACTCGAATACGCATTGCCACACAGTTATGCTCAGCCCTTGTATTTCTTCACACAAACAAACTCTGCAGGGTTATTCACTGCGACTTGAATCCTGCTAATATACTACTAGATGGCAACTACATTCCCAAACTTGCAGGATTTGGCAATTGTCATTTTTTACCTAACGATGAAACTACATCTGTCAGAGCTGACATAGAGAGCAACCATGAGTCTCCGCTCACAACAAAGTTGGACGTTTTTTCATTTGGCATAGTATTGTTAAGGTTGTTAACAGGAAAATCAGACATGATGATAAAATTATTACATGAGGTTCAATTTGCCATAGATGGCCGAAAATTGAAAGATGTATTGGATCCACGAGCCGGGGACTGGCCGTTCGTGCAAGCCGAACAGTTGGCCCAATTGGCATTTAGGTGTTGCAATACAAATAGTATGAAAAGGCCAGACCTTGCGTCTGACGTGTGGAGAGTACTTGAGCCAATGAGGGCTTCTTGTGGAGTTTCACTATCAGTATGCCTAGGCTCTGGGGAGCAACAACTGCAACCTCCTCCTTATTTCATATGCCCCATTTTTCAAGTAAGTTTTATTATTACCTTAAGCCTCTCTCTCGGTTTTCTCATAATTCTTGCTTTGATGATGGTGTTTTAGTTAAAGGATATGTAATTGTTGTAAATGCAAGTTTCTGAACTGTTTCATGTATATCTCTTTGCATATTCTTTTCATTGATGGAACCTCCTAGCTAAAGTTGAGTTGATTTTCTGGCCTTTCTTATTATTCACATCGTTCCTCAATACAGTTCTTTATGGGATCCTGGTTAAATACATTGTTGGTCTCGAGTTTTCCATCCATTTTTTCAGTTTAGTCCTTGAACTTGTAAGAATTTTTCTATTTCGGTCACTAGACTTTGATCATAAATCTTTAAGAATCTCATTTTTAGTTTAATTTATTTAGAAGTGGTTTAACATCATTCATTATACCTTATCATTTTTTCACGGTAGAGACAACATGTGGAGCTGAATGAAAATTAAATGATAAAGTTGTTGAAGGATTAAAATAGGGAGGGGTTAAAAGTTTAGAGATTAAAATAGAAAAATCTTAGTTATTGGCTGGGAAATACACATCTTCTATGTACATTATTCTTCTTTGCACCCACACCTCTGATGGATTGATATTTCATTCCTTCCATTGGCAAATATTCAGGAAGTCATGGAGGATCCTCATGTGGCAGCCGACGGGTTCACCTACGAGGCGGAGGCATTGAGAGGATGGTTGGACAGTGGGCATGACACTTCGCCAATGACGAACCTTAGGCTCGACCACCGAAACCTCGTCCCTAACCGTGCTCTTCGTTCTGCAATTCAGGAATGGTTGCACCAGCCCCAGTAGTAAGCCCATTGATTCATTTTTGTGTTGGTTTTGTGTTTCTGTTTCCATATAGTTAATCTCTGCCATTACAGAAGTAGGGGAAAAAGAAAAGGAAGAGATAGAGTGAAAGAGTGGAAGGGAAACAGTAGATATGTAGGTCCTGTTCGGTAATGATTTCGTTTTTAGTTTTCTATTTTTAAAAATTATGCTTATTTCCTCTTAATTTCCTTGAAAACACATTTAAATTCTTACTCAAATATCAAAAAATAAAATGGGTTGTTAATACTTACTTTATTTAGATTTTGAAAACACTTTTAAAAGATACATAACACAGCAAAGAAACATATAAGTGGAAGTAGTGTTTATAATTAAAATTTTCAAAAACAGAAAACTAAAAACTAAATGGTTACCAAACGGGACCGTGAAAAAATAAACGAGATATGGAAAAAGGAAATGGGGCTGGTAAAGAATCTGTTTTCCTTTTTGTTTGTGGTGGGTTCCTAAAGTTTGGAGAATGAATCTGCTACCATATCCTTTTCCTGTTGGGTTGCAGTAATATAGAAAAAGTGTGAATATATATATATATATATACGTAAATAAAAATGTGTGTGTATAATCTGTTGCTTGTATTTGATTTGATTTGATGACTTAAATTCCAGGCAAGTTGGTCATTAGCAAGTGCTATTTCAGACAAATCCTTTTCACCTTCTATCAAGTTAAATCAGTCATTGAATGTTGGCTTTTTTTTTTCTTTTTTAAATATCATTTTTAACTCTTATTTGGCAAGTTGCATCCATTTTTATTCCAGAATTTTATCAAATTCACCTTAGATTTGGATAAATATTACAATTTTCATCTAACTTTGAAAAGGTGTTGCAATTTTGTTTCAACTCAAGTCTCGTGCCTACTAAGTATTAAGTTCATTGATGGTTTCAATATTGTATTGGTTATCGTGCCCTTGACTTTGGTTCAAAATACTTTATCTTTTAGGTGCGTGTACTTTTAC

mRNA sequence

GTTGATGTGTTGAATAAGTTGAGACATCCAAATATTATGACGCTCATTGGAGCTTGCTCGGAGTCTTGCATTCTTGTTTATGACTATTTCGCCAATGGAAGCCTAGAAGATAGGCTAGCCTGCAAGGACAATTCTTCCCCATTATCATGGAAAACTCGAATACGCATTGCCACACAGTTATGCTCAGCCCTTGTATTTCTTCACACAAACAAACTCTGCAGGGTTATTCACTGCGACTTGAATCCTGCTAATATACTACTAGATGGCAACTACATTCCCAAACTTGCAGGATTTGGCAATTGTCATTTTTTACCTAACGATGAAACTACATCTGTCAGAGCTGACATAGAGAGCAACCATGAGTCTCCGCTCACAACAAAGTTGGACGTTTTTTCATTTGGCATAGTATTGTTAAGGTTGTTAACAGGAAAATCAGACATGATGATAAAATTATTACATGAGGTTCAATTTGCCATAGATGGCCGAAAATTGAAAGATGTATTGGATCCACGAGCCGGGGACTGGCCGTTCGTGCAAGCCGAACAGTTGGCCCAATTGGCATTTAGGTGTTGCAATACAAATAGTATGAAAAGGCCAGACCTTGCGTCTGACGTGTGGAGAGTACTTGAGCCAATGAGGGCTTCTTGTGGAGTTTCACTATCAGTATGCCTAGGCTCTGGGGAGCAACAACTGCAACCTCCTCCTTATTTCATATGCCCCATTTTTCAAGAAGTCATGGAGGATCCTCATGTGGCAGCCGACGGGTTCACCTACGAGGCGGAGGCATTGAGAGGATGGTTGGACAGTGGGCATGACACTTCGCCAATGACGAACCTTAGGCTCGACCACCGAAACCTCGTCCCTAACCGTGCTCTTCGTTCTGCAATTCAGGAATGGTTGCACCAGCCCCAGTAGTAAGCCCATTGATTCATTTTTGTGTTGGTTTTGTGTTTCTGTTTCCATATAGTTAATCTCTGCCATTACAGAAGTAGGGGAAAAAGAAAAGGAAGAGATAGAGTGAAAGAGTGGAAGGGAAACAGTAGATATGTAGGTCCTGTTCGGTAATGATTTCGTTTTTAGTTTTCTATTTTTAAAAATTATGCTTATTTCCTCTTAATTTCCTTGAAAACACATTTAAATTCTTACTCAAATATCAAAAAATAAAATGGGTTGTTAATACTTACTTTATTTAGATTTTGAAAACACTTTTAAAAGATACATAACACAGCAAAGAAACATATAAGTGGAAGTAGTGTTTATAATTAAAATTTTCAAAAACAGAAAACTAAAAACTAAATGGTTACCAAACGGGACCGTGAAAAAATAAACGAGATATGGAAAAAGGAAATGGGGCTGGTAAAGAATCTGTTTTCCTTTTTGTTTGTGGTGGGTTCCTAAAGTTTGGAGAATGAATCTGCTACCATATCCTTTTCCTGTTGGGTTGCAGTAATATAGAAAAAGTGTGAATATATATATATATATATACGTAAATAAAAATGTGTGTGTATAATCTGTTGCTTGTATTTGATTTGATTTGATGACTTAAATTCCAGGCAAGTTGGTCATTAGCAAGTGCTATTTCAGACAAATCCTTTTCACCTTCTATCAAGTTAAATCAGTCATTGAATGTTGGCTTTTTTTTTTCTTTTTTAAATATCATTTTTAACTCTTATTTGGCAAGTTGCATCCATTTTTATTCCAGAATTTTATCAAATTCACCTTAGATTTGGATAAATATTACAATTTTCATCTAACTTTGAAAAGGTGTTGCAATTTTGTTTCAACTCAAGTCTCGTGCCTACTAAGTATTAAGTTCATTGATGGTTTCAATATTGTATTGGTTATCGTGCCCTTGACTTTGGTTCAAAATACTTTATCTTTTAGGTGCGTGTACTTTTAC

Coding sequence (CDS)

GTTGATGTGTTGAATAAGTTGAGACATCCAAATATTATGACGCTCATTGGAGCTTGCTCGGAGTCTTGCATTCTTGTTTATGACTATTTCGCCAATGGAAGCCTAGAAGATAGGCTAGCCTGCAAGGACAATTCTTCCCCATTATCATGGAAAACTCGAATACGCATTGCCACACAGTTATGCTCAGCCCTTGTATTTCTTCACACAAACAAACTCTGCAGGGTTATTCACTGCGACTTGAATCCTGCTAATATACTACTAGATGGCAACTACATTCCCAAACTTGCAGGATTTGGCAATTGTCATTTTTTACCTAACGATGAAACTACATCTGTCAGAGCTGACATAGAGAGCAACCATGAGTCTCCGCTCACAACAAAGTTGGACGTTTTTTCATTTGGCATAGTATTGTTAAGGTTGTTAACAGGAAAATCAGACATGATGATAAAATTATTACATGAGGTTCAATTTGCCATAGATGGCCGAAAATTGAAAGATGTATTGGATCCACGAGCCGGGGACTGGCCGTTCGTGCAAGCCGAACAGTTGGCCCAATTGGCATTTAGGTGTTGCAATACAAATAGTATGAAAAGGCCAGACCTTGCGTCTGACGTGTGGAGAGTACTTGAGCCAATGAGGGCTTCTTGTGGAGTTTCACTATCAGTATGCCTAGGCTCTGGGGAGCAACAACTGCAACCTCCTCCTTATTTCATATGCCCCATTTTTCAAGAAGTCATGGAGGATCCTCATGTGGCAGCCGACGGGTTCACCTACGAGGCGGAGGCATTGAGAGGATGGTTGGACAGTGGGCATGACACTTCGCCAATGACGAACCTTAGGCTCGACCACCGAAACCTCGTCCCTAACCGTGCTCTTCGTTCTGCAATTCAGGAATGGTTGCACCAGCCCCAGTAG

Protein sequence

VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQLCSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWLHQPQ

Homology

[Close]

BLAST of MC07g0352 vs. ExPASy Swiss-Prot
Match: Q8GUH1 (U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2 SV=2)

HSP 1 Score: 398.7 bits (1023), Expect = 6.0e-110
Identity = 194/309 (62.78%), Postives = 235/309 (76.05%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+K+RHPNI+TLIGAC E   LVY+Y   GSLEDRL CKDNS PLSW+ R+RIAT++
Sbjct: 525 VDVLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEI 584

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADI---- 120
           C+ALVFLH+NK   ++H DL PANILLD N + KL+ FG C  L  + + SVR D+    
Sbjct: 585 CAALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTV 644

Query: 121 -----ESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPR 180
                E++    LT K DV+SFGI+LLRLLTG+    +++ +EV++A+D   L D+LDP 
Sbjct: 645 AYLDPEASSSGELTPKSDVYSFGIILLRLLTGRP--ALRISNEVKYALDNGTLNDLLDPL 704

Query: 181 AGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQL 240
           AGDWPFVQAEQLA+LA RCC T S  RPDL ++VWRVLEPMRAS G S S  LG  E ++
Sbjct: 705 AGDWPFVQAEQLARLALRCCETVSENRPDLGTEVWRVLEPMRASSGGSSSFHLGRNEHRI 764

Query: 241 QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRA 300
             PPYFICPIFQEVM+DPHVAADGFTYEAEA+R WLDS HDTSPMTN++L H +L+ N A
Sbjct: 765 -APPYFICPIFQEVMQDPHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHA 824

[Close]

BLAST of MC07g0352 vs. ExPASy Swiss-Prot
Match: Q5WA76 (U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB70 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.4e-84
Identity = 159/315 (50.48%), Postives = 205/315 (65.08%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           V +L+++RHP+++TL+GACSES  LVY++  NGSLED L C D    L+W+ RIRI  ++
Sbjct: 489 VSILSRVRHPHLVTLLGACSESSTLVYEFLPNGSLEDFLMCSDKRQTLTWQARIRIIAEI 548

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDET----------- 120
           CSAL+FLH NK   V+H DL PANILL  N + KL+ FG    L    T           
Sbjct: 549 CSALIFLHKNKPHPVVHGDLKPANILLGVNLVSKLSDFGISRLLIQSSTNNTTLYRTMHP 608

Query: 121 --TSVRADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDV 180
             T +  D E      LT + DV+SFGIV+LRLLTGK  + IK +  V+ A++   L  V
Sbjct: 609 VGTPLYMDPEFLSTGELTPQSDVYSFGIVVLRLLTGKPPVGIKNI--VEDAMEKGDLNSV 668

Query: 181 LDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSG 240
           +D   G+WP +  EQLA LA RC   +   RPDL+ +VW ++E +R    +S      S 
Sbjct: 669 IDTSVGEWPHLHIEQLAYLALRCTELSRRCRPDLSGEVWAIVEAIR-DAALSSPSSSRSA 728

Query: 241 EQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLV 300
           + Q  PP YFICPI Q++M+DPH+AADGFTYEAEA+R WL +GHDTSPMTNL L+H  L+
Sbjct: 729 QDQNSPPSYFICPISQDIMDDPHIAADGFTYEAEAIRSWLCNGHDTSPMTNLLLEHEELI 788

Query: 301 PNRALRSAIQEWLHQ 303
           PNRALRSAIQEWL Q
Sbjct: 789 PNRALRSAIQEWLQQ 800

[Close]

BLAST of MC07g0352 vs. ExPASy Swiss-Prot
Match: Q94A51 (U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-74
Identity = 141/306 (46.08%), Postives = 208/306 (67.97%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           V++L+++RHPN++TL+GAC ES  L+Y Y  NGSLED  + ++N   LSW++RIRIA+++
Sbjct: 504 VEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESRIRIASEI 563

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH 120
           CSAL+FLH+N  C +IH +L P+ ILLD N + K+  +G    +P D        ++ ++
Sbjct: 564 CSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHY 623

Query: 121 --ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFV 180
                +T + D+++FGI+LL+LLT +   +  +L +V+ A++   +  VLD  AGDWP  
Sbjct: 624 FVSREMTLESDIYAFGIILLQLLTRRP--VSGILRDVKCALENDNISAVLDNSAGDWPVA 683

Query: 181 QAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQL--QPPPY 240
           + ++LA +A RCC  N M RPDLA  V R ++ M+A   V  S       Q +  +PP +
Sbjct: 684 RGKKLANVAIRCCKKNPMNRPDLAV-VLRFIDRMKAP-EVPSSETSSYANQNVPRRPPSH 743

Query: 241 FICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAI 300
           ++CPIFQEVM+DP +AADGFTYEAEA+R WL +GHDTSPMTNL+++  NL+PN AL  AI
Sbjct: 744 YLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAI 803

Query: 301 QEWLHQ 303
           Q+W +Q
Sbjct: 804 QDWQNQ 804

[Close]

BLAST of MC07g0352 vs. ExPASy Swiss-Prot
Match: Q0DR28 (U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB57 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.0e-74
Identity = 143/311 (45.98%), Postives = 187/311 (60.13%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           V +L + RHPNI+T IG CSE   LVY++  NG+LEDR+ C +NS+PLSW  R +I  ++
Sbjct: 198 VSILRQWRHPNIITFIGVCSEVSALVYEWLPNGNLEDRIICTNNSAPLSWYNRTQIIGEI 257

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCH-------FLPNDETTSVR 120
           C AL+FLH+NK   ++H DL P NIL+D NY  K+  FG  +       F PN       
Sbjct: 258 CCALLFLHSNKSTALVHGDLRPCNILIDANYRSKICNFGMSNLFLQLGTFPPNLTARLPY 317

Query: 121 ADIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAG 180
            D E N    LTT  DV+S G+++LRLLTG     + L  +V  A+    L  ++D  AG
Sbjct: 318 MDPEFNTTGELTTLSDVYSLGVIILRLLTGMPP--LTLSEKVAEALGSDSLHLLIDKSAG 377

Query: 181 DWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQP 240
           DWP+++A+QLA +   C      KRPDL ++VW V+EP+      +    L S       
Sbjct: 378 DWPYIEAKQLALIGLSCTGMTRKKRPDLLNEVWIVIEPLTRKPPAATWPYLQSASGDSSV 437

Query: 241 PPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALR 300
           P  FICPI  E+M+DP VA+DGFTYEAEA+R W D G   SPMTNL L + NLVPNR LR
Sbjct: 438 PAAFICPISMEIMKDPQVASDGFTYEAEAIRCWFDRGISRSPMTNLALPNLNLVPNRVLR 497

Query: 301 SAIQEWLHQPQ 305
           S I  +L Q Q
Sbjct: 498 SFIHGYLQQQQ 506

[Close]

BLAST of MC07g0352 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 233.0 bits (593), Expect = 4.4e-60
Identity = 128/314 (40.76%), Postives = 184/314 (58.60%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           +++L+K+RHP+++ L+GAC E   LVY+Y  NGSLEDRL   +NS PL W  R RIA ++
Sbjct: 524 LEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEV 583

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFG----------NCHFLPNDETT 120
            +ALVFLH +K   +IH DL PANILLD N++ K+   G          +  F    +T+
Sbjct: 584 AAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTS 643

Query: 121 SVRA----DIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGR-KLK 180
            V      D E      +++K D++SFG++LL+LLT K    I L H V+ A+D   +  
Sbjct: 644 PVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKP--AIALTHFVESAMDSNDEFL 703

Query: 181 DVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLG 240
            +LD +AG+WP  +  +LA LA  C       RPDL   +   LE ++     + +   G
Sbjct: 704 KILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKARNSFSG 763

Query: 241 SGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRN 300
                 QPP +FICP+ ++VM +P VAADG+TY+  A+  WL   H+TSPMT+  L  +N
Sbjct: 764 ---VSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEWLKE-HNTSPMTDSPLHSKN 823

[Close]

BLAST of MC07g0352 vs. NCBI nr
Match: XP_022157677.1 (U-box domain-containing protein 33-like [Momordica charantia])

HSP 1 Score: 629 bits (1623), Expect = 5.79e-220
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL
Sbjct: 519 VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 578

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH 120
           CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH
Sbjct: 579 CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH 638

Query: 121 ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQA 180
           ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQA
Sbjct: 639 ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQA 698

Query: 181 EQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICP 240
           EQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICP
Sbjct: 699 EQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICP 758

Query: 241 IFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL 300
           IFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL
Sbjct: 759 IFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL 818

Query: 301 HQPQ 304
           HQPQ
Sbjct: 819 HQPQ 822

[Close]

BLAST of MC07g0352 vs. NCBI nr
Match: XP_038907096.1 (U-box domain-containing protein 33-like isoform X2 [Benincasa hispida])

HSP 1 Score: 506 bits (1303), Expect = 2.61e-173
Identity = 243/312 (77.88%), Postives = 272/312 (87.18%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+KLRHPNI TLIG C E+CILVYDYF NG+LEDRLACKDNSSPLSWKTRIRIAT+L
Sbjct: 384 VDVLSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRIRIATEL 443

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIES-- 120
           CSAL+F+H+NK+C++IH D+ P+N+LLD NY+PKLAGFG CHFLP+DE +S   D+ +  
Sbjct: 444 CSALIFIHSNKVCKIIHGDMKPSNVLLDANYVPKLAGFGTCHFLPHDEKSSYNEDLSAGY 503

Query: 121 ----NHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAID--GRKLKDVLDPRA 180
               NHE PLTT+LDVFSFG++LL LLTG+S + +K   +VQF I+   RKLKDVLDPRA
Sbjct: 504 DAKGNHEFPLTTELDVFSFGMILLSLLTGQSYLRLK--EDVQFIINIKKRKLKDVLDPRA 563

Query: 181 GDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQ 240
           GDWPFVQAEQLAQLA RCCNTNSM RPDL SDVWRVLEPMRASCG S SVCL  G+QQ+Q
Sbjct: 564 GDWPFVQAEQLAQLALRCCNTNSMYRPDLVSDVWRVLEPMRASCGGSQSVCLSFGDQQVQ 623

Query: 241 PPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRAL 300
           PPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDH+NLVPNRAL
Sbjct: 624 PPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRAL 683

Query: 301 RSAIQEWLHQPQ 304
           RS IQEWL Q Q
Sbjct: 684 RSVIQEWLQQHQ 693

[Close]

BLAST of MC07g0352 vs. NCBI nr
Match: XP_038907095.1 (U-box domain-containing protein 33-like isoform X1 [Benincasa hispida])

HSP 1 Score: 506 bits (1303), Expect = 1.05e-171
Identity = 243/312 (77.88%), Postives = 272/312 (87.18%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+KLRHPNI TLIG C E+CILVYDYF NG+LEDRLACKDNSSPLSWKTRIRIAT+L
Sbjct: 506 VDVLSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRIRIATEL 565

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIES-- 120
           CSAL+F+H+NK+C++IH D+ P+N+LLD NY+PKLAGFG CHFLP+DE +S   D+ +  
Sbjct: 566 CSALIFIHSNKVCKIIHGDMKPSNVLLDANYVPKLAGFGTCHFLPHDEKSSYNEDLSAGY 625

Query: 121 ----NHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAID--GRKLKDVLDPRA 180
               NHE PLTT+LDVFSFG++LL LLTG+S + +K   +VQF I+   RKLKDVLDPRA
Sbjct: 626 DAKGNHEFPLTTELDVFSFGMILLSLLTGQSYLRLK--EDVQFIINIKKRKLKDVLDPRA 685

Query: 181 GDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQ 240
           GDWPFVQAEQLAQLA RCCNTNSM RPDL SDVWRVLEPMRASCG S SVCL  G+QQ+Q
Sbjct: 686 GDWPFVQAEQLAQLALRCCNTNSMYRPDLVSDVWRVLEPMRASCGGSQSVCLSFGDQQVQ 745

Query: 241 PPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRAL 300
           PPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDH+NLVPNRAL
Sbjct: 746 PPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRAL 805

Query: 301 RSAIQEWLHQPQ 304
           RS IQEWL Q Q
Sbjct: 806 RSVIQEWLQQHQ 815

[Close]

BLAST of MC07g0352 vs. NCBI nr
Match: XP_023547656.1 (U-box domain-containing protein 33-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501 bits (1290), Expect = 2.41e-171
Identity = 239/310 (77.10%), Postives = 271/310 (87.42%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+ LRHPNI TLIG C E+C+LVYDYF NGSLEDRLACKDNSSPLSWKTRIRIA++L
Sbjct: 386 VDVLSILRHPNIATLIGVCPEACVLVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASEL 445

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADI---- 120
           CSAL+F+H+NK+C++IH DL P N+LLD NY+PKLAGFG+CHFLP+DE  S   +I    
Sbjct: 446 CSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENIPAKY 505

Query: 121 --ESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGD 180
             +SNHE PLTTKLDVFSFG+VLL LLTGKS + +K   +VQFA++ RKLK+VLDPRAGD
Sbjct: 506 GVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYLRMK--EDVQFAVEERKLKNVLDPRAGD 565

Query: 181 WPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPP 240
           WPFVQAEQLAQLA RCCN NSM RPDL SDVWRVLEPM+ASCG S+SV L  G+ Q+QPP
Sbjct: 566 WPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPP 625

Query: 241 PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRS 300
           PYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+H+NLVPNRALRS
Sbjct: 626 PYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRS 685

Query: 301 AIQEWLHQPQ 304
            IQEWLHQ Q
Sbjct: 686 VIQEWLHQQQ 693

[Close]

BLAST of MC07g0352 vs. NCBI nr
Match: XP_022959087.1 (U-box domain-containing protein 33-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 500 bits (1287), Expect = 6.84e-171
Identity = 241/310 (77.74%), Postives = 270/310 (87.10%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+ LRHPNI TLIG C E+CILVYDYF NGSLEDRLACKDNSSPLSWKTRIRIA++L
Sbjct: 386 VDVLSILRHPNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASEL 445

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADI---- 120
           CSAL+F+H+NK+C++IH DL P N+LLD NY+PKLAGFG+CHFLP+DE  S   +I    
Sbjct: 446 CSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKY 505

Query: 121 --ESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGD 180
             +SN E PLTTKLDVFSFG+VLL LLTGKS + +K   +VQFAI+ RKLKDVLDPRAGD
Sbjct: 506 GVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSYLRMK--EDVQFAIEERKLKDVLDPRAGD 565

Query: 181 WPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPP 240
           WPFVQAEQLAQLA RCCN NSM RPDL SDVWRVLEPM+ASCG S+SV L  G+ Q+QPP
Sbjct: 566 WPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPP 625

Query: 241 PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRS 300
           PYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+H+NLVPNRALRS
Sbjct: 626 PYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRS 685

Query: 301 AIQEWLHQPQ 304
            IQEWLHQ Q
Sbjct: 686 VIQEWLHQQQ 693

[Close]

BLAST of MC07g0352 vs. ExPASy TrEMBL
Match: A0A6J1DV42 (U-box domain-containing protein 33-like OS=Momordica charantia OX=3673 GN=LOC111024334 PE=4 SV=1)

HSP 1 Score: 629 bits (1623), Expect = 2.80e-220
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL
Sbjct: 519 VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 578

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH 120
           CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH
Sbjct: 579 CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH 638

Query: 121 ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQA 180
           ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQA
Sbjct: 639 ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFVQA 698

Query: 181 EQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICP 240
           EQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICP
Sbjct: 699 EQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPPPYFICP 758

Query: 241 IFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL 300
           IFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL
Sbjct: 759 IFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAIQEWL 818

Query: 301 HQPQ 304
           HQPQ
Sbjct: 819 HQPQ 822

[Close]

BLAST of MC07g0352 vs. ExPASy TrEMBL
Match: A0A6J1H3L1 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 3.31e-171
Identity = 241/310 (77.74%), Postives = 270/310 (87.10%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+ LRHPNI TLIG C E+CILVYDYF NGSLEDRLACKDNSSPLSWKTRIRIA++L
Sbjct: 386 VDVLSILRHPNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASEL 445

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADI---- 120
           CSAL+F+H+NK+C++IH DL P N+LLD NY+PKLAGFG+CHFLP+DE  S   +I    
Sbjct: 446 CSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKY 505

Query: 121 --ESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGD 180
             +SN E PLTTKLDVFSFG+VLL LLTGKS + +K   +VQFAI+ RKLKDVLDPRAGD
Sbjct: 506 GVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSYLRMK--EDVQFAIEERKLKDVLDPRAGD 565

Query: 181 WPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPP 240
           WPFVQAEQLAQLA RCCN NSM RPDL SDVWRVLEPM+ASCG S+SV L  G+ Q+QPP
Sbjct: 566 WPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPP 625

Query: 241 PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRS 300
           PYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+H+NLVPNRALRS
Sbjct: 626 PYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRS 685

Query: 301 AIQEWLHQPQ 304
            IQEWLHQ Q
Sbjct: 686 VIQEWLHQQQ 693

[Close]

BLAST of MC07g0352 vs. ExPASy TrEMBL
Match: A0A6J1H3V9 (E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1)

HSP 1 Score: 500 bits (1287), Expect = 1.31e-169
Identity = 241/310 (77.74%), Postives = 270/310 (87.10%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+ LRHPNI TLIG C E+CILVYDYF NGSLEDRLACKDNSSPLSWKTRIRIA++L
Sbjct: 508 VDVLSILRHPNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASEL 567

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADI---- 120
           CSAL+F+H+NK+C++IH DL P N+LLD NY+PKLAGFG+CHFLP+DE  S   +I    
Sbjct: 568 CSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHFLPHDEKLSYNENISAKY 627

Query: 121 --ESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGD 180
             +SN E PLTTKLDVFSFG+VLL LLTGKS + +K   +VQFAI+ RKLKDVLDPRAGD
Sbjct: 628 GVKSNPEFPLTTKLDVFSFGMVLLSLLTGKSYLRMK--EDVQFAIEERKLKDVLDPRAGD 687

Query: 181 WPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPP 240
           WPFVQAEQLAQLA RCCN NSM RPDL SDVWRVLEPM+ASCG S+SV L  G+ Q+QPP
Sbjct: 688 WPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPP 747

Query: 241 PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRS 300
           PYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+H+NLVPNRALRS
Sbjct: 748 PYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRS 807

Query: 301 AIQEWLHQPQ 304
            IQEWLHQ Q
Sbjct: 808 VIQEWLHQQQ 815

[Close]

BLAST of MC07g0352 vs. ExPASy TrEMBL
Match: A0A0A0KD01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004520 PE=4 SV=1)

HSP 1 Score: 499 bits (1286), Expect = 1.75e-169
Identity = 241/310 (77.74%), Postives = 266/310 (85.81%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VD L+KLRHPNI TLIG C E+CILVYDYF NG+LEDRLACKDNSSPLSWKTRI IATQL
Sbjct: 506 VDALSKLRHPNIATLIGVCPEACILVYDYFPNGNLEDRLACKDNSSPLSWKTRILIATQL 565

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDE------TTSVRA 120
           CSAL+F+H+NK+C+ +H D+ P+NILLD NY+PKLAGFG CHFL +DE        SV  
Sbjct: 566 CSALIFIHSNKICKTVHGDMKPSNILLDANYVPKLAGFGTCHFLTHDEKLSYNENLSVGY 625

Query: 121 DIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGD 180
           D + NHE PLTT+LD++SFG VLL LLTGKS + +K   +VQ AI  RKLKDVLDPRAGD
Sbjct: 626 DAKRNHEFPLTTELDIYSFGTVLLSLLTGKSYLRMK--EDVQIAIKKRKLKDVLDPRAGD 685

Query: 181 WPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPP 240
           WPFVQAEQLAQLA RCC+T+SM RPDL SDVWRVLEPMRASCG SLSVCL  G+QQ+QPP
Sbjct: 686 WPFVQAEQLAQLALRCCDTDSMYRPDLVSDVWRVLEPMRASCGGSLSVCLSFGDQQVQPP 745

Query: 241 PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRS 300
           PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDH+NLVPNRALRS
Sbjct: 746 PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHQNLVPNRALRS 805

Query: 301 AIQEWLHQPQ 304
            IQEWL Q Q
Sbjct: 806 VIQEWLQQHQ 813

[Close]

BLAST of MC07g0352 vs. ExPASy TrEMBL
Match: A0A6J1L2C9 (E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111499216 PE=4 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 5.24e-169
Identity = 239/310 (77.10%), Postives = 268/310 (86.45%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VD L+ +RHPNI TLIG C E+CILVYDYF NGSLEDRLACKDNSSPLSWKTRIRIA+ L
Sbjct: 508 VDALSIMRHPNIATLIGVCPEACILVYDYFPNGSLEDRLACKDNSSPLSWKTRIRIASDL 567

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADI---- 120
           CSAL+F+H+NK+C++IH DL P N+LLD NY+PKLAGFG+CH LP+DE  S   +I    
Sbjct: 568 CSALIFIHSNKICKIIHGDLKPLNVLLDANYVPKLAGFGSCHLLPHDEKLSYNENISAKY 627

Query: 121 --ESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGD 180
             +SNHE PLTTKLDVFSFG+VLL LLTGKS + +K   +VQFAI+ RKLKDVLDPRAGD
Sbjct: 628 GVKSNHEFPLTTKLDVFSFGMVLLSLLTGKSYLRMK--EDVQFAIEERKLKDVLDPRAGD 687

Query: 181 WPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQLQPP 240
           WPFVQAEQLAQLA RCCN NSM RPDL SDVWRVLEPM+ASCG S+SV L  G+ Q+QPP
Sbjct: 688 WPFVQAEQLAQLALRCCNRNSMYRPDLVSDVWRVLEPMKASCGGSVSVSLSFGDLQVQPP 747

Query: 241 PYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRS 300
           PYF+CPIFQEVMEDPHVAADGFTYEAEALRGWLDSGH+TSPMTNLRL+H+NLVPNRALRS
Sbjct: 748 PYFLCPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHNTSPMTNLRLEHQNLVPNRALRS 807

Query: 301 AIQEWLHQPQ 304
            IQEWLHQ Q
Sbjct: 808 VIQEWLHQQQ 815

[Close]

BLAST of MC07g0352 vs. TAIR 10
Match: AT2G45910.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 398.7 bits (1023), Expect = 4.3e-111
Identity = 194/309 (62.78%), Postives = 235/309 (76.05%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           VDVL+K+RHPNI+TLIGAC E   LVY+Y   GSLEDRL CKDNS PLSW+ R+RIAT++
Sbjct: 525 VDVLSKMRHPNIITLIGACPEGWSLVYEYLPGGSLEDRLTCKDNSPPLSWQNRVRIATEI 584

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADI---- 120
           C+ALVFLH+NK   ++H DL PANILLD N + KL+ FG C  L  + + SVR D+    
Sbjct: 585 CAALVFLHSNKAHSLVHGDLKPANILLDSNLVSKLSDFGTCSLLHPNGSKSVRTDVTGTV 644

Query: 121 -----ESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPR 180
                E++    LT K DV+SFGI+LLRLLTG+    +++ +EV++A+D   L D+LDP 
Sbjct: 645 AYLDPEASSSGELTPKSDVYSFGIILLRLLTGRP--ALRISNEVKYALDNGTLNDLLDPL 704

Query: 181 AGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQL 240
           AGDWPFVQAEQLA+LA RCC T S  RPDL ++VWRVLEPMRAS G S S  LG  E ++
Sbjct: 705 AGDWPFVQAEQLARLALRCCETVSENRPDLGTEVWRVLEPMRASSGGSSSFHLGRNEHRI 764

Query: 241 QPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRA 300
             PPYFICPIFQEVM+DPHVAADGFTYEAEA+R WLDS HDTSPMTN++L H +L+ N A
Sbjct: 765 -APPYFICPIFQEVMQDPHVAADGFTYEAEAIRAWLDSEHDTSPMTNVKLSHTSLIANHA 824

[Close]

BLAST of MC07g0352 vs. TAIR 10
Match: AT3G49060.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 281.6 bits (719), Expect = 7.7e-76
Identity = 141/306 (46.08%), Postives = 208/306 (67.97%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           V++L+++RHPN++TL+GAC ES  L+Y Y  NGSLED  + ++N   LSW++RIRIA+++
Sbjct: 504 VEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESRIRIASEI 563

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH 120
           CSAL+FLH+N  C +IH +L P+ ILLD N + K+  +G    +P D        ++ ++
Sbjct: 564 CSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHY 623

Query: 121 --ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFV 180
                +T + D+++FGI+LL+LLT +   +  +L +V+ A++   +  VLD  AGDWP  
Sbjct: 624 FVSREMTLESDIYAFGIILLQLLTRRP--VSGILRDVKCALENDNISAVLDNSAGDWPVA 683

Query: 181 QAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQL--QPPPY 240
           + ++LA +A RCC  N M RPDLA  V R ++ M+A   V  S       Q +  +PP +
Sbjct: 684 RGKKLANVAIRCCKKNPMNRPDLAV-VLRFIDRMKAP-EVPSSETSSYANQNVPRRPPSH 743

Query: 241 FICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAI 300
           ++CPIFQEVM+DP +AADGFTYEAEA+R WL +GHDTSPMTNL+++  NL+PN AL  AI
Sbjct: 744 YLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAI 803

Query: 301 QEWLHQ 303
           Q+W +Q
Sbjct: 804 QDWQNQ 804

[Close]

BLAST of MC07g0352 vs. TAIR 10
Match: AT3G49060.2 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 281.6 bits (719), Expect = 7.7e-76
Identity = 141/306 (46.08%), Postives = 208/306 (67.97%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           V++L+++RHPN++TL+GAC ES  L+Y Y  NGSLED  + ++N   LSW++RIRIA+++
Sbjct: 494 VEILSRVRHPNLVTLMGACPESRSLIYQYIPNGSLEDCFSSENNVPALSWESRIRIASEI 553

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFGNCHFLPNDETTSVRADIESNH 120
           CSAL+FLH+N  C +IH +L P+ ILLD N + K+  +G    +P D        ++ ++
Sbjct: 554 CSALLFLHSNIPC-IIHGNLKPSKILLDSNLVTKINDYGISQLIPIDGLDKSDPHVDPHY 613

Query: 121 --ESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGRKLKDVLDPRAGDWPFV 180
                +T + D+++FGI+LL+LLT +   +  +L +V+ A++   +  VLD  AGDWP  
Sbjct: 614 FVSREMTLESDIYAFGIILLQLLTRRP--VSGILRDVKCALENDNISAVLDNSAGDWPVA 673

Query: 181 QAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLGSGEQQL--QPPPY 240
           + ++LA +A RCC  N M RPDLA  V R ++ M+A   V  S       Q +  +PP +
Sbjct: 674 RGKKLANVAIRCCKKNPMNRPDLAV-VLRFIDRMKAP-EVPSSETSSYANQNVPRRPPSH 733

Query: 241 FICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRNLVPNRALRSAI 300
           ++CPIFQEVM+DP +AADGFTYEAEA+R WL +GHDTSPMTNL+++  NL+PN AL  AI
Sbjct: 734 YLCPIFQEVMKDPLIAADGFTYEAEAIREWLANGHDTSPMTNLKMEDCNLIPNHALHLAI 793

Query: 301 QEWLHQ 303
           Q+W +Q
Sbjct: 794 QDWQNQ 794

[Close]

BLAST of MC07g0352 vs. TAIR 10
Match: AT4G25160.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 233.0 bits (593), Expect = 3.1e-61
Identity = 128/314 (40.76%), Postives = 184/314 (58.60%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           +++L+K+RHP+++ L+GAC E   LVY+Y  NGSLEDRL   +NS PL W  R RIA ++
Sbjct: 524 LEILSKIRHPHLVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEV 583

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFG----------NCHFLPNDETT 120
            +ALVFLH +K   +IH DL PANILLD N++ K+   G          +  F    +T+
Sbjct: 584 AAALVFLHKSKPKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTS 643

Query: 121 SVRA----DIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGR-KLK 180
            V      D E      +++K D++SFG++LL+LLT K    I L H V+ A+D   +  
Sbjct: 644 PVGTLCYIDPEYQRTGRISSKSDIYSFGMILLQLLTAKP--AIALTHFVESAMDSNDEFL 703

Query: 181 DVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSVCLG 240
            +LD +AG+WP  +  +LA LA  C       RPDL   +   LE ++     + +   G
Sbjct: 704 KILDQKAGNWPIEETRELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKARNSFSG 763

Query: 241 SGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLDHRN 300
                 QPP +FICP+ ++VM +P VAADG+TY+  A+  WL   H+TSPMT+  L  +N
Sbjct: 764 ---VSTQPPTHFICPLLKDVMNEPCVAADGYTYDRHAIEEWLKE-HNTSPMTDSPLHSKN 823

[Close]

BLAST of MC07g0352 vs. TAIR 10
Match: AT5G51270.1 (U-box domain-containing protein kinase family protein )

HSP 1 Score: 231.5 bits (589), Expect = 9.1e-61
Identity = 123/322 (38.20%), Postives = 193/322 (59.94%), Query Frame = 0


Query: 1   VDVLNKLRHPNIMTLIGACSESCILVYDYFANGSLEDRLACKDNSSPLSWKTRIRIATQL 60
           +++L+K+RHP+++ L+GAC +   LVY+Y  NGSLEDRL   ++S P+ W  R+RIA ++
Sbjct: 504 LEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIPWFVRLRIAWEV 563

Query: 61  CSALVFLHTNKLCRVIHCDLNPANILLDGNYIPKLAGFG-----------NCHFLPNDET 120
            SALVFLH +K   +IH DL PANILL+ N++ K+   G           +  F    +T
Sbjct: 564 ASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADPLSTKFTMYKQT 623

Query: 121 TSVRA----DIESNHESPLTTKLDVFSFGIVLLRLLTGKSDMMIKLLHEVQFAIDGR--- 180
           + V      D E      ++ K DV++FG+++L+LLTG+  M   L + V+ A++     
Sbjct: 624 SPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAM--ALTYTVETAMENNNDD 683

Query: 181 KLKDVLDPRAGDWPFVQAEQLAQLAFRCCNTNSMKRPDLASDVWRVLEPMRASCGVSLSV 240
           +L  +LD +AG+WP  +  QLA LA +C    S  RPDL   +  VLE ++     + + 
Sbjct: 684 ELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLKKVADKARNS 743

Query: 241 CLGSGEQQLQPPPYFICPIFQEVMEDPHVAADGFTYEAEALRGWLDSGHDTSPMTNLRLD 300
              +     QPP +F CP+ ++VM++P +AADG+TY+  A+  W+++ H TSP+TN  L 
Sbjct: 744 LSAAPS---QPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEEWMEN-HRTSPVTNSPLQ 803

Query: 301 HRNLVPNRALRSAIQEWLHQPQ 305
           + NL+PN  L +AI EW ++ Q
Sbjct: 804 NVNLLPNHTLYAAIVEWRNRNQ 819

The following BLAST results are available for this feature:

BLAST of MC07g0352 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GUH1	6.0e-110	62.78	U-box domain-containing protein 33 OS=Arabidopsis thaliana OX=3702 GN=PUB33 PE=2...	[more]
Q5WA76	4.4e-84	50.48	U-box domain-containing protein 70 OS=Oryza sativa subsp. japonica OX=39947 GN=P...	[more]
Q94A51	1.1e-74	46.08	U-box domain-containing protein 32 OS=Arabidopsis thaliana OX=3702 GN=PUB32 PE=1...	[more]
Q0DR28	7.0e-74	45.98	U-box domain-containing protein 57 OS=Oryza sativa subsp. japonica OX=39947 GN=P...	[more]
Q9SW11	4.4e-60	40.76	U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2...	[more]

BLAST of MC07g0352 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157677.1	5.79e-220	100.00	U-box domain-containing protein 33-like [Momordica charantia]	[more]
XP_038907096.1	2.61e-173	77.88	U-box domain-containing protein 33-like isoform X2 [Benincasa hispida]	[more]
XP_038907095.1	1.05e-171	77.88	U-box domain-containing protein 33-like isoform X1 [Benincasa hispida]	[more]
XP_023547656.1	2.41e-171	77.10	U-box domain-containing protein 33-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022959087.1	6.84e-171	77.74	U-box domain-containing protein 33-like isoform X2 [Cucurbita moschata]	[more]

BLAST of MC07g0352 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DV42	2.80e-220	100.00	U-box domain-containing protein 33-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1H3L1	3.31e-171	77.74	E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1	[more]
A0A6J1H3V9	1.31e-169	77.74	E3 ubiquitin ligase OS=Cucurbita moschata OX=3662 GN=LOC111460185 PE=4 SV=1	[more]
A0A0A0KD01	1.75e-169	77.74	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G004520 PE=4 SV=1	[more]
A0A6J1L2C9	5.24e-169	77.10	E3 ubiquitin ligase OS=Cucurbita maxima OX=3661 GN=LOC111499216 PE=4 SV=1	[more]

BLAST of MC07g0352 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45910.1	4.3e-111	62.78	U-box domain-containing protein kinase family protein	[more]
AT3G49060.1	7.7e-76	46.08	U-box domain-containing protein kinase family protein	[more]
AT3G49060.2	7.7e-76	46.08	U-box domain-containing protein kinase family protein	[more]
AT4G25160.1	3.1e-61	40.76	U-box domain-containing protein kinase family protein	[more]
AT5G51270.1	9.1e-61	38.20	U-box domain-containing protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 236..299 e-value: 5.4E-29 score: 112.3
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 234..302 e-value: 2.2E-18 score: 66.2
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 232..304 score: 37.737522
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 1..33 e-value: 3.1E-6 score: 29.3
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 34..220 e-value: 5.8E-37 score: 129.0
None	No IPR available	PIRSR	PIRSR037921-2	PIRSR037921-2	coord: 2..101 e-value: 1.7E-14 score: 51.7
None	No IPR available	PANTHER	PTHR45647	OS02G0152300 PROTEIN	coord: 1..299
None	No IPR available	PANTHER	PTHR45647:SF100	U-BOX DOMAIN-CONTAINING PROTEIN 33	coord: 1..299
None	No IPR available	CDD	cd16655	RING-Ubox_WDSUB1_like	coord: 237..278 e-value: 2.17742E-25 score: 93.8227
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 227..302
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 2..200 e-value: 6.0E-24 score: 84.8
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..213 score: 20.188311
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 231..304 e-value: 9.9E-29 score: 101.1
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 2..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0352.1	MC07g0352.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity