MC07g0265 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0265
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	naringenin,2-oxoglutarate 3-dioxygenase-like
Location	MC07: 9062338 .. 9065460 (+)
RNA-Seq Expression	MC07g0265
Synteny	MC07g0265

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AACGAAATCCCTATCATTTCACTTGTTGGAATCGACGATGAAGTCAATGGCAGAAGAGGTGATATTAAGAAGAAGATCGCTGAGGCTTGCGAGGTATGGGGGATTTTCCAAGTTGTAGACCATGGGGTTGACGCTAAACTTGTGTCTGATATGATTCATCTTGCCAAAGATTTTTTTGACATGTCATCACAAGAGAAGCTCCGCTTCGACATGTCGAGTGGTAAGAGAGGTGGTTTTCTAGCCACCACCCACCTAAAGGTAATATAATATAACAACTCTTACGTATAAGTATAAGGGGTTGTTTGGTAATATTTATGTTTTTTATATATGTTTTCTGTTTCTTATTTCCTATGCTCTTACAAAACGTATAAGGGGTTGTTCGGTAATACTTATGTTTATCGTATTTTATTTTTTTTTTCTTATTTATAAGTTTTGAGAAAGACTATTTTTTCTTTCTAAGTTCTTGTTTATGAGAGCCATTACCAAAGTTCTATACAAATTTTGGAAACATTAAAGAAATTTTTATATTTGTCACTTTTTAAAACTTAATTATATAATGCATATTAATTATTAAATATTAAATATATAACAATAATTCTAATATATATATAATTTTTTTAAAAGAATTATTTTTCATTTAAATATTTAAAATAACAATAATTCTAATATATATATATATTTTTTTAAAAGAATTATTTTTCATTTAAATCTTTAAAATTTTTTAAATATCACAAATAATAAATGGTTACCAATGCTGGGTAATATTTCTATTTTTTGTATTTTTTTATATGTTTTTCATCTTTGTTGATTTTATTTCTCGTTTTCAAGGAAGAAAAACAATACCATGTTGAGTAATTATTTTTGTAATCTCTTTTCTCCTCTTCATCGAAAAAAGTAGGAATATGCTAGCTAATGCTAATCATTTGTAGATTTTAAATTAAGAAAAAAAAAAACTTCAAAATTTTACAAGTTATTTTGTTTTCTTTATTTTTTCGTTGAGCTCATTTCTGTAAGGGTTCCATTGTTTAATGATGGTAAGTAGGAGATACCTACCTCATGAGCACTTATTTTTTATCCTCATATTGCAGGAAGAAGCAAAACGATATTCGCGAGAAATTGTAACATTCTTTACGTACCCAATTGAAAATCGAGACTACTCGTTGTGGCCAGATAAGCCCGAAGGGTGGGCGGCCACGACCGCAGAGTACGGCGAGAAAATGATGGATTTGGCCTGTAAGTTGCTAGAGGTGCTATCAGAGGCCATGAGAATGGAGAAGGAAGCGATAAAGAAAGCCTGTTTGGAAATGTACAAGAAGATTTTGGTCAACTTTTACCCTAAATGTGGAGACTCCAATGATGTTGTTGTGGGGCTACAGCGCCGCAGTGACCCTGGAACCATAACCATATTGTTACAGGATCATGTGTGTGGGCTGCAGGCTACCAAAGACGGTGGCAAGTCGTGGGTCGACGTCGCACCCTTGAAAGGAGCCTTTGTCGTCAACATTGGAGACCATGGTCATGTAAGCAAGCTTCTCTGTTACGATTTCAAATTTTATTTTGGTTGGTATACTATTAGCTTTTCGCATTAGGTATATGATTTCTGTTAAGATTTTGTAGAAAAAATGGGTTATAAAGGTCACTTTTTTCAAGGTACGTGAAGTATAATGATTGATTTGAAAGTATAGTAAAATGGATAGAAACTAAAGATATAGGAACCGAAATAAACATTTTGAAAATATGGTCTTAGATAGAAAAAGCTGAAGGTTTAAGGAAGATTTAGACCTAATAATAACCCTATTCAGAAAAGAAAAAAGAGTTGAGTTCTTTAATTTTGAACAAATAATTCGAATAAACCCACCCAACCTAAATATTCCATGCTTGGATCGGGTTCAATCTACCATGTAACTAGAGTCATTTAAGTTAATTTATCATTCAAATAATTGAGTCCAAAAAGCCCTTTTTATTATTTAAGTTTGACATTGCAATTTTTTTTTGCTTTGAAAGTTTTATGCTAATTTATTTCCAACCTCAGAGACTAAAAATGTATTTTACCCTAAATTATAAGTTAAATTATAGATTTGGTCCTCAATTTTTAATATAGTGTTCACTTAGTCCTTTTATATACTTTGAAAAGTTTTAACCATCATGGGTGGCTTATTAGTCGAAAGGGGCTAGGGTAATTAAGAGGGAATGAGTTCAAATCCCATAATGGCCACCTACCTTAGGAATTAGTAATTCCTATGTATTTCTCAGCACCAAATGTTGTAGGGCAGTGCCCCGTGAGATAGTCTCTACATGGTACACACTAACTAACTGTATTATTTAGGGCATGTTTGGTAATGTTCATGTTTCTTGTTTCTTTTTCCTCGTTTTTAATAAAGAAAGATAGAATTGCATGGTCTCAATACTCACGAATTAAAGAATGATCAAACAATATTTATATTGATATTTTGTAATAAAATTGGAAACAGTAACAAAAAACTTCTTTTCTTATTTCCAAAATTTCTATACAAATTTTGAGAATGTTTTAAAAAATATGAGAAACAAGAATATAATTAGTTGTAAAATGCTTAGGATTCTTAATAAAATTGGAAATAAGAAATAAGAAACGAGAACATTGCCAAACAAGTCCTTAATGATTAGGTAGCAAGTTTATTTTTGGGCAAGTTTAGTCGGTTAGTGGCGTAAGAATTAAACTAACAAAGTTCATGAACAAGGACTTGTACAAGACCCAAATTTGAAAAATTCATGAATTTATTGGAATCTTTTTAAAGTACAGGGACTAAATAAATAGAATACGATCTTTACAATGGTAAAATATAAGAACTAAATAAACAGTATGACTTATAATTTAACTTGAATATTAAAGCAAACTATTGAGTTGATGGTAATTAGAATGATTCGTGAAAATAGTATCTTAGCAATGGGAGGTTCAAGAATGGTGATCATCAAGTAGTGGCAAGCTCAAACACCAGCAGAGTATCCATAGCCTCATTTCATAACCCAAAACCAGAAGCCATTGTGTATCCATTGAAGGTCAGTGAAGACGAGAAGCCAATGCTTGAGGAGCCCATCACCTATGGTGAGATGTATACAAGAAAGATGAACAAAGAAATACCTCTA

mRNA sequence

AACGAAATCCCTATCATTTCACTTGTTGGAATCGACGATGAAGTCAATGGCAGAAGAGGTGATATTAAGAAGAAGATCGCTGAGGCTTGCGAGGTATGGGGGATTTTCCAAGTTGTAGACCATGGGGTTGACGCTAAACTTGTGTCTGATATGATTCATCTTGCCAAAGATTTTTTTGACATGTCATCACAAGAGAAGCTCCGCTTCGACATGTCGAGTGGTAAGAGAGGTGGTTTTCTAGCCACCACCCACCTAAAGGAAGAAGCAAAACGATATTCGCGAGAAATTGTAACATTCTTTACGTACCCAATTGAAAATCGAGACTACTCGTTGTGGCCAGATAAGCCCGAAGGGTGGGCGGCCACGACCGCAGAGTACGGCGAGAAAATGATGGATTTGGCCTGTAAGTTGCTAGAGGTGCTATCAGAGGCCATGAGAATGGAGAAGGAAGCGATAAAGAAAGCCTGTTTGGAAATGTACAAGAAGATTTTGGTCAACTTTTACCCTAAATGTGGAGACTCCAATGATGTTGTTGTGGGGCTACAGCGCCGCAGTGACCCTGGAACCATAACCATATTGTTACAGGATCATGTGTGTGGGCTGCAGGCTACCAAAGACGGTGGCAAGTCGTGGGTCGACGTCGCACCCTTGAAAGGAGCCTTTGTCGTCAACATTGGAGACCATGGTCATTATCTTAGCAATGGGAGGTTCAAGAATGGTGATCATCAAGTAGTGGCAAGCTCAAACACCAGCAGAGTATCCATAGCCTCATTTCATAACCCAAAACCAGAAGCCATTGTGTATCCATTGAAGGTCAGTGAAGACGAGAAGCCAATGCTTGAGGAGCCCATCACCTATGGTGAGATGTATACAAGAAAGATGAACAAAGAAATACCTCTA

Coding sequence (CDS)

AACGAAATCCCTATCATTTCACTTGTTGGAATCGACGATGAAGTCAATGGCAGAAGAGGTGATATTAAGAAGAAGATCGCTGAGGCTTGCGAGGTATGGGGGATTTTCCAAGTTGTAGACCATGGGGTTGACGCTAAACTTGTGTCTGATATGATTCATCTTGCCAAAGATTTTTTTGACATGTCATCACAAGAGAAGCTCCGCTTCGACATGTCGAGTGGTAAGAGAGGTGGTTTTCTAGCCACCACCCACCTAAAGGAAGAAGCAAAACGATATTCGCGAGAAATTGTAACATTCTTTACGTACCCAATTGAAAATCGAGACTACTCGTTGTGGCCAGATAAGCCCGAAGGGTGGGCGGCCACGACCGCAGAGTACGGCGAGAAAATGATGGATTTGGCCTGTAAGTTGCTAGAGGTGCTATCAGAGGCCATGAGAATGGAGAAGGAAGCGATAAAGAAAGCCTGTTTGGAAATGTACAAGAAGATTTTGGTCAACTTTTACCCTAAATGTGGAGACTCCAATGATGTTGTTGTGGGGCTACAGCGCCGCAGTGACCCTGGAACCATAACCATATTGTTACAGGATCATGTGTGTGGGCTGCAGGCTACCAAAGACGGTGGCAAGTCGTGGGTCGACGTCGCACCCTTGAAAGGAGCCTTTGTCGTCAACATTGGAGACCATGGTCATTATCTTAGCAATGGGAGGTTCAAGAATGGTGATCATCAAGTAGTGGCAAGCTCAAACACCAGCAGAGTATCCATAGCCTCATTTCATAACCCAAAACCAGAAGCCATTGTGTATCCATTGAAGGTCAGTGAAGACGAGAAGCCAATGCTTGAGGAGCCCATCACCTATGGTGAGATGTATACAAGAAAGATGAACAAAGAAATACCTCTA

Protein sequence

NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFDMSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWAATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVGLQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNGDHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL

Homology

[Close]

BLAST of MC07g0265 vs. ExPASy Swiss-Prot
Match: Q05965 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 GN=FHT PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.4e-114
Identity = 194/300 (64.67%), Postives = 246/300 (82.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +EIP+ISL GIDD V+G+RG+I ++I EACE WGIFQVVDHGVD  LV+DM  LA+DFF 
Sbjct: 35  DEIPVISLAGIDD-VDGKRGEICREIVEACENWGIFQVVDHGVDTSLVADMTRLARDFFA 94

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           +  +EKLRFDMS GK+GGF+ ++HL+ EA +  REIVT+F+YP+ NRDYS WPDKP+GWA
Sbjct: 95  LPPEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPQGWA 154

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
             T EY EK+M LACKLLEVLSEAM +EKE++  AC++M +KI+VN+YPKC    D+ +G
Sbjct: 155 KVTEEYSEKLMGLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKC-PQPDLTLG 214

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQAT+D G +W+ V P++GAFVVN+GDHGH+LSNGRFKN 
Sbjct: 215 LKRHTDPGTITLLLQDQVGGLQATRDDGNTWITVQPVEGAFVVNLGDHGHFLSNGRFKNA 274

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SN+SR+SIA+F NP PEA VYPLKV E EK ++EEPIT+ EMY RKM +++ L
Sbjct: 275 DHQAVVNSNSSRLSIATFQNPAPEATVYPLKVREGEKAIMEEPITFAEMYKRKMGRDLEL 332

[Close]

BLAST of MC07g0265 vs. ExPASy Swiss-Prot
Match: Q9S818 (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 1.8e-114
Identity = 193/300 (64.33%), Postives = 246/300 (82.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +EIP+ISL GIDD V+G+RG+I ++I EACE WGIFQVVDHGVD  LV+DM  LA+DFF 
Sbjct: 36  DEIPVISLAGIDD-VDGKRGEICRQIVEACENWGIFQVVDHGVDTNLVADMTRLARDFFA 95

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           +  ++KLRFDMS GK+GGF+ ++HL+ EA +  REIVT+F+YP+ NRDYS WPDKPEGW 
Sbjct: 96  LPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWV 155

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
             T EY E++M LACKLLEVLSEAM +EKE++  AC++M +KI+VN+YPKC    D+ +G
Sbjct: 156 KVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKC-PQPDLTLG 215

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQAT+D GK+W+ V P++GAFVVN+GDHGH+LSNGRFKN 
Sbjct: 216 LKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNA 275

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SN+SR+SIA+F NP P+A VYPLKV E EK +LEEPIT+ EMY RKM +++ L
Sbjct: 276 DHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYKRKMGRDLEL 333

[Close]

BLAST of MC07g0265 vs. ExPASy Swiss-Prot
Match: Q07353 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN=AN3 PE=1 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 5.2e-114
Identity = 193/300 (64.33%), Postives = 246/300 (82.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           NEIPIISL GIDDE  G+R +I  KI +ACE WG+FQVVDHGVDA+++S M   AK+FF 
Sbjct: 39  NEIPIISLEGIDDE-TGKRAEICDKIVKACEDWGVFQVVDHGVDAEVISQMTTFAKEFFA 98

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           +  +EKLRFDMS GK+GGF+ ++HL+ E  +  REIVT+F+YP   RDYS WPDKPEGW 
Sbjct: 99  LPPEEKLRFDMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTRARDYSRWPDKPEGWI 158

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           A T +Y EK+M+LACKLL+VLSEAM +EKEA+ KAC++M +K++VNFYPKC +  D+ +G
Sbjct: 159 AVTQKYSEKLMELACKLLDVLSEAMGLEKEALTKACVDMDQKVVVNFYPKCPEP-DLTLG 218

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKD GK+W+ V P++GAFVVN+GDHGH+LSNGRFKN 
Sbjct: 219 LKRHTDPGTITLLLQDQVGGLQATKDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNA 278

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SN+SR+SIA+F NP PEAIVYPLK+ E EK +++EPIT+ EMY RKM+K++ L
Sbjct: 279 DHQAVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKSIMDEPITFAEMYRRKMSKDLEL 336

[Close]

BLAST of MC07g0265 vs. ExPASy Swiss-Prot
Match: A0A4D6Q4T7 (Flavanone 3-dioxygenase F3H2 OS=Crocosmia x crocosmiiflora OX=1053288 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.4e-113
Identity = 191/300 (63.67%), Postives = 246/300 (82.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +EIP+ISL GI DEV+GRR +I KKI  ACE WG+FQVVDHGVDA L+SDM  LA++FF 
Sbjct: 41  SEIPVISLEGI-DEVDGRRAEICKKIVAACEDWGVFQVVDHGVDAGLISDMTRLAREFFA 100

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           +  ++KLRFDM+ GK+GGF+ ++HL+ EA +  REIVT+F+YPI  RDYS WPDKPE W 
Sbjct: 101 LPPEDKLRFDMTGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRARDYSRWPDKPEDWR 160

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           + T  Y EK+M+LACKLL +LSEAM ++ EA+ KAC++M +K++VNFYPKC    D+ +G
Sbjct: 161 SVTKTYSEKLMELACKLLGILSEAMGLDTEALTKACVDMDQKVVVNFYPKC-PQPDLTLG 220

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKDGGK+W+ V P++GAFVVN+GDHGH+LSNGRFKN 
Sbjct: 221 LKRHTDPGTITLLLQDQVGGLQATKDGGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNA 280

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SNTSR+SIA+F NP P+AIVYPL + E EKP+L+EPIT+ EMY RKM++++ L
Sbjct: 281 DHQAVVNSNTSRLSIATFQNPAPDAIVYPLAIREGEKPVLDEPITFAEMYRRKMSRDLEL 338

[Close]

BLAST of MC07g0265 vs. ExPASy Swiss-Prot
Match: A0A4D6Q9B0 (Flavanone 3-dioxygenase F3H1 OS=Crocosmia x crocosmiiflora OX=1053288 GN=F3H-1 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.2e-112
Identity = 190/300 (63.33%), Postives = 245/300 (81.67%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +EIP+ISL GI DE+ GRR +I KKI  ACE WG+FQVVDHGVDA +++DM  LA++FF 
Sbjct: 41  SEIPVISLEGI-DEIEGRRVEICKKIVAACEDWGVFQVVDHGVDAGVIADMTRLAREFFS 100

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           +  ++KLRFDMS GK+GGF+ ++HL+ EA +  REIVT+F+YPI  RDYS WPDKPE W 
Sbjct: 101 LPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRARDYSRWPDKPEDWR 160

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           + T +Y E +M+LACKLL VLSEAM ++ EAI KAC++M +K++VNFYPKC    D+ +G
Sbjct: 161 SVTEKYSETLMELACKLLGVLSEAMGLDTEAITKACIDMDQKVVVNFYPKC-PQPDLTLG 220

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKDGGK+W+ V P++GAFVVN+GDHGH+LSNGRFKN 
Sbjct: 221 LKRHTDPGTITLLLQDQVGGLQATKDGGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNA 280

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SNTSR+SIA+F NP P+AIVYPL + E EKP+L+EPIT+ EMY RKM++++ L
Sbjct: 281 DHQAVVNSNTSRLSIATFQNPAPDAIVYPLAIREGEKPVLDEPITFAEMYRRKMSRDLEL 338

[Close]

BLAST of MC07g0265 vs. NCBI nr
Match: XP_022156653.1 (naringenin,2-oxoglutarate 3-dioxygenase-like [Momordica charantia])

HSP 1 Score: 612 bits (1578), Expect = 1.29e-220
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD
Sbjct: 18  NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 77

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA
Sbjct: 78  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 137

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG
Sbjct: 138 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 197

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG
Sbjct: 198 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 257

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL
Sbjct: 258 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 317

[Close]

BLAST of MC07g0265 vs. NCBI nr
Match: XP_022158003.1 (naringenin,2-oxoglutarate 3-dioxygenase-like [Momordica charantia])

HSP 1 Score: 504 bits (1299), Expect = 4.17e-178
Identity = 244/300 (81.33%), Postives = 272/300 (90.67%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           NEIP+ISL GIDDEVNGRRGDIKKKI +ACEVWGIFQVVDHGVDAKLVSDMIHLAK FFD
Sbjct: 18  NEIPVISLAGIDDEVNGRRGDIKKKIVKACEVWGIFQVVDHGVDAKLVSDMIHLAKQFFD 77

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           M SQEKL FDMSSGK+GG+LATTH+K +A R SREIVTFFTYPI++RDYSLWPDKPEGWA
Sbjct: 78  MPSQEKLCFDMSSGKKGGYLATTHVKGDAIRDSREIVTFFTYPIKSRDYSLWPDKPEGWA 137

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
             T EY EK+M LA KLL VLSEAM ++KEAI+KACLEM +KI+ NFYPK GDSNDV  G
Sbjct: 138 TMTMEYSEKLMGLASKLLAVLSEAMGLDKEAIEKACLEMNQKIVANFYPKIGDSNDVAQG 197

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           LQR +D GTITILLQDHVCGLQATKDGG+SWVDV PL+GAFV++IGDHGHYLSNGR+K+G
Sbjct: 198 LQRHTDTGTITILLQDHVCGLQATKDGGESWVDVEPLEGAFVIDIGDHGHYLSNGRYKSG 257

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQVVASSN+SRVSI SFHNPK EAIVYPLKVSE++KPMLEE IT+GEMYTR ++KE+PL
Sbjct: 258 DHQVVASSNSSRVSIVSFHNPKAEAIVYPLKVSEEDKPMLEERITFGEMYTRVISKELPL 317

[Close]

BLAST of MC07g0265 vs. NCBI nr
Match: KAG7028205.1 (Naringenin,2-oxoglutarate 3-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 430 bits (1106), Expect = 1.99e-148
Identity = 203/300 (67.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +E+PIISL GIDD   GRR +I +KI EACE WGIFQVVDHGVD+ L+++M  LA++FF 
Sbjct: 36  DEVPIISLAGIDDVAGGRRAEICRKIVEACEDWGIFQVVDHGVDSDLITEMTRLAREFFA 95

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           + ++EKLRFDMS GK+GGF+ ++HLK E  +  REIVT+F+YPI NRDY+ WPDKPEGWA
Sbjct: 96  LPAEEKLRFDMSGGKKGGFIVSSHLKGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWA 155

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           A TAEY EK+M LACKLLEVLSEAM +EKEA+ KAC++M +KI+VNFYPKC    D+ +G
Sbjct: 156 ARTAEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKIVVNFYPKCLQP-DLTLG 215

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKDGGKSW+ V P+ GAFVVN+GDHGHYLSNGRFKN 
Sbjct: 216 LKRHTDPGTITLLLQDQVGGLQATKDGGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNA 275

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SNTSR+SIA+F NP PEAIVYPLKV E EKP+LE PIT+ +MY  KM++++ L
Sbjct: 276 DHQAVVNSNTSRLSIATFQNPAPEAIVYPLKVREGEKPILENPITFSQMYQTKMSRDLEL 334

[Close]

BLAST of MC07g0265 vs. NCBI nr
Match: XP_023539914.1 (naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 430 bits (1106), Expect = 2.72e-148
Identity = 203/300 (67.67%), Postives = 248/300 (82.67%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +E+PIISL GIDD   GRR +I +KI EACE WGIFQVVDHGVD+ L+S+M  LA++FF 
Sbjct: 36  DEVPIISLAGIDDVTGGRRAEICRKIVEACEDWGIFQVVDHGVDSDLISEMTRLAREFFA 95

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           + ++EKLRFDMS GK+GGF+ ++HLK E  +  REIVT+F+YPI NRDY+ WPDKPEGWA
Sbjct: 96  LPAEEKLRFDMSGGKKGGFIVSSHLKGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWA 155

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
             TAEY EK+M LACKLLEVLSEAM +EKEA+ KAC++M +KI+VNFYPKC    D+ +G
Sbjct: 156 TRTAEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKIVVNFYPKCVQP-DLTLG 215

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKDGGKSW+ V P+ GAFVVN+GDHGHYLSNGRFKN 
Sbjct: 216 LKRHTDPGTITLLLQDQVGGLQATKDGGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNA 275

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SNTSR+SIA+F NP PEAIVYPLKV E EKP+LE PIT+ +MY  KM++++ L
Sbjct: 276 DHQAVVNSNTSRLSIATFQNPAPEAIVYPLKVREGEKPILENPITFSQMYQTKMSRDLEL 334

[Close]

BLAST of MC07g0265 vs. NCBI nr
Match: XP_022939196.1 (naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita moschata])

HSP 1 Score: 430 bits (1106), Expect = 3.73e-148
Identity = 203/300 (67.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +E+PIISL GIDD   GRR +I +KI EACE WGIFQVVDHGVD+ L+++M  LA++FF 
Sbjct: 36  DEVPIISLAGIDDVAGGRRAEICRKIVEACEDWGIFQVVDHGVDSDLITEMTRLAREFFA 95

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           + ++EKLRFDMS GK+GGF+ ++HLK E  +  REIVT+F+YPI NRDY+ WPDKPEGWA
Sbjct: 96  LPAEEKLRFDMSGGKKGGFIVSSHLKGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWA 155

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           A TAEY EK+M LACKLLEVLSEAM +EKEA+ KAC++M +KI+VNFYPKC    D+ +G
Sbjct: 156 ARTAEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKIVVNFYPKCLQP-DLTLG 215

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKDGGKSW+ V P+ GAFVVN+GDHGHYLSNGRFKN 
Sbjct: 216 LKRHTDPGTITLLLQDQVGGLQATKDGGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNA 275

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SNTSR+SIA+F NP PEAIVYPLKV E EKP+LE PIT+ +MY  KM++++ L
Sbjct: 276 DHQAVVNSNTSRLSIATFQNPAPEAIVYPLKVREGEKPILENPITFSQMYQTKMSRDLEL 334

[Close]

BLAST of MC07g0265 vs. ExPASy TrEMBL
Match: A0A6J1DR74 (naringenin,2-oxoglutarate 3-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111023502 PE=3 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 6.25e-221
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD
Sbjct: 18  NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 77

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA
Sbjct: 78  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 137

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG
Sbjct: 138 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 197

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG
Sbjct: 198 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 257

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL
Sbjct: 258 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 317

[Close]

BLAST of MC07g0265 vs. ExPASy TrEMBL
Match: A0A6J1DUW5 (naringenin,2-oxoglutarate 3-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111024602 PE=3 SV=1)

HSP 1 Score: 504 bits (1299), Expect = 2.02e-178
Identity = 244/300 (81.33%), Postives = 272/300 (90.67%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           NEIP+ISL GIDDEVNGRRGDIKKKI +ACEVWGIFQVVDHGVDAKLVSDMIHLAK FFD
Sbjct: 18  NEIPVISLAGIDDEVNGRRGDIKKKIVKACEVWGIFQVVDHGVDAKLVSDMIHLAKQFFD 77

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           M SQEKL FDMSSGK+GG+LATTH+K +A R SREIVTFFTYPI++RDYSLWPDKPEGWA
Sbjct: 78  MPSQEKLCFDMSSGKKGGYLATTHVKGDAIRDSREIVTFFTYPIKSRDYSLWPDKPEGWA 137

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
             T EY EK+M LA KLL VLSEAM ++KEAI+KACLEM +KI+ NFYPK GDSNDV  G
Sbjct: 138 TMTMEYSEKLMGLASKLLAVLSEAMGLDKEAIEKACLEMNQKIVANFYPKIGDSNDVAQG 197

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           LQR +D GTITILLQDHVCGLQATKDGG+SWVDV PL+GAFV++IGDHGHYLSNGR+K+G
Sbjct: 198 LQRHTDTGTITILLQDHVCGLQATKDGGESWVDVEPLEGAFVIDIGDHGHYLSNGRYKSG 257

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQVVASSN+SRVSI SFHNPK EAIVYPLKVSE++KPMLEE IT+GEMYTR ++KE+PL
Sbjct: 258 DHQVVASSNSSRVSIVSFHNPKAEAIVYPLKVSEEDKPMLEERITFGEMYTRVISKELPL 317

[Close]

BLAST of MC07g0265 vs. ExPASy TrEMBL
Match: A0A6J1FF87 (naringenin,2-oxoglutarate 3-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111445185 PE=3 SV=1)

HSP 1 Score: 430 bits (1106), Expect = 1.80e-148
Identity = 203/300 (67.67%), Postives = 249/300 (83.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +E+PIISL GIDD   GRR +I +KI EACE WGIFQVVDHGVD+ L+++M  LA++FF 
Sbjct: 36  DEVPIISLAGIDDVAGGRRAEICRKIVEACEDWGIFQVVDHGVDSDLITEMTRLAREFFA 95

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           + ++EKLRFDMS GK+GGF+ ++HLK E  +  REIVT+F+YPI NRDY+ WPDKPEGWA
Sbjct: 96  LPAEEKLRFDMSGGKKGGFIVSSHLKGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWA 155

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           A TAEY EK+M LACKLLEVLSEAM +EKEA+ KAC++M +KI+VNFYPKC    D+ +G
Sbjct: 156 ARTAEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKIVVNFYPKCLQP-DLTLG 215

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKDGGKSW+ V P+ GAFVVN+GDHGHYLSNGRFKN 
Sbjct: 216 LKRHTDPGTITLLLQDQVGGLQATKDGGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNA 275

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SNTSR+SIA+F NP PEAIVYPLKV E EKP+LE PIT+ +MY  KM++++ L
Sbjct: 276 DHQAVVNSNTSRLSIATFQNPAPEAIVYPLKVREGEKPILENPITFSQMYQTKMSRDLEL 334

[Close]

BLAST of MC07g0265 vs. ExPASy TrEMBL
Match: A0A6J1KVH7 (naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111498563 PE=3 SV=1)

HSP 1 Score: 429 bits (1104), Expect = 3.05e-148
Identity = 204/300 (68.00%), Postives = 247/300 (82.33%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +E+PIISL GIDD   GRR +I +KI EACE WGIFQVVDHGVD  L+S+M  LA++FF 
Sbjct: 36  DEVPIISLAGIDDVAGGRRAEICRKIVEACEDWGIFQVVDHGVDLDLISEMTRLAREFFA 95

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           + ++EKLRFDMS GK+GGF+ ++HLK E  +  REIVT+F+YPI NRDY+ WPDKPEGWA
Sbjct: 96  LPAEEKLRFDMSGGKKGGFIVSSHLKGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWA 155

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           A T EY EK+M LACKLLEVLSEAM +EKEA+ KAC++M +KI+VNFYPKC    D+ +G
Sbjct: 156 ARTVEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKIVVNFYPKCLQP-DLTLG 215

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQATKDGGKSW+ V P+ GAFVVN+GDHGHYLSNGRFKN 
Sbjct: 216 LKRHTDPGTITLLLQDQVGGLQATKDGGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNA 275

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SNTSR+SIA+F NP PEAIVYPLKV E EKP+LE PIT+ EMY  KM++++ L
Sbjct: 276 DHQAVVNSNTSRLSIATFQNPAPEAIVYPLKVREGEKPILENPITFSEMYQIKMSRDLEL 334

[Close]

BLAST of MC07g0265 vs. ExPASy TrEMBL
Match: A0A6P8DVK5 (naringenin,2-oxoglutarate 3-dioxygenase OS=Punica granatum OX=22663 GN=LOC116211316 PE=3 SV=1)

HSP 1 Score: 427 bits (1099), Expect = 1.88e-147
Identity = 203/300 (67.67%), Postives = 248/300 (82.67%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           NEIP+ISL GIDDE    R +I +KI EACE WGIFQVVDHGVDAKLV DM  LA++FF 
Sbjct: 38  NEIPVISLKGIDDEDGPARAEICRKIVEACEDWGIFQVVDHGVDAKLVQDMTRLAREFFA 97

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           + ++EKLRFDMS GK+GGF+ ++HL+ EA +  REIVT+F+YPI  RDYS WPDKPEGW 
Sbjct: 98  LPAEEKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPIRARDYSRWPDKPEGWK 157

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
           + T EY EK+M LACKLLEVLSEAM +EKEA+ KAC++M +K++VN+YPKC    D+ +G
Sbjct: 158 SVTMEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKVVVNYYPKCPQP-DLTLG 217

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQAT+DGGK+W+ V P++GAFVVN+GDHGHYLSNGRFKN 
Sbjct: 218 LKRHTDPGTITLLLQDQVGGLQATRDGGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNA 277

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SN SR+SIA+F NP PEAIVYPLKV E EKP+LEEPIT+ EMY RKM+K++ L
Sbjct: 278 DHQAVVNSNCSRLSIATFQNPAPEAIVYPLKVREGEKPILEEPITFAEMYRRKMSKDLEL 336

[Close]

BLAST of MC07g0265 vs. TAIR 10
Match: AT3G51240.1 (flavanone 3-hydroxylase )

HSP 1 Score: 413.7 bits (1062), Expect = 1.3e-115
Identity = 193/300 (64.33%), Postives = 246/300 (82.00%), Query Frame = 0


Query: 1   NEIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFD 60
           +EIP+ISL GIDD V+G+RG+I ++I EACE WGIFQVVDHGVD  LV+DM  LA+DFF 
Sbjct: 36  DEIPVISLAGIDD-VDGKRGEICRQIVEACENWGIFQVVDHGVDTNLVADMTRLARDFFA 95

Query: 61  MSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 120
           +  ++KLRFDMS GK+GGF+ ++HL+ EA +  REIVT+F+YP+ NRDYS WPDKPEGW 
Sbjct: 96  LPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWV 155

Query: 121 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 180
             T EY E++M LACKLLEVLSEAM +EKE++  AC++M +KI+VN+YPKC    D+ +G
Sbjct: 156 KVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPKC-PQPDLTLG 215

Query: 181 LQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNG 240
           L+R +DPGTIT+LLQD V GLQAT+D GK+W+ V P++GAFVVN+GDHGH+LSNGRFKN 
Sbjct: 216 LKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNA 275

Query: 241 DHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMYTRKMNKEIPL 300
           DHQ V +SN+SR+SIA+F NP P+A VYPLKV E EK +LEEPIT+ EMY RKM +++ L
Sbjct: 276 DHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYKRKMGRDLEL 333

[Close]

BLAST of MC07g0265 vs. TAIR 10
Match: AT3G51240.2 (flavanone 3-hydroxylase )

HSP 1 Score: 342.8 bits (878), Expect = 2.8e-94
Identity = 158/250 (63.20%), Postives = 203/250 (81.20%), Query Frame = 0


Query: 51  MIHLAKDFFDMSSQEKLRFDMSSGKRGGFLATTHLKEEAKRYSREIVTFFTYPIENRDYS 110
           M  LA+DFF +  ++KLRFDMS GK+GGF+ ++HL+ EA +  REIVT+F+YP+ NRDYS
Sbjct: 1   MTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYS 60

Query: 111 LWPDKPEGWAATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPK 170
            WPDKPEGW   T EY E++M LACKLLEVLSEAM +EKE++  AC++M +KI+VN+YPK
Sbjct: 61  RWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPK 120

Query: 171 CGDSNDVVVGLQRRSDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGH 230
           C    D+ +GL+R +DPGTIT+LLQD V GLQAT+D GK+W+ V P++GAFVVN+GDHGH
Sbjct: 121 C-PQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGH 180

Query: 231 YLSNGRFKNGDHQVVASSNTSRVSIASFHNPKPEAIVYPLKVSEDEKPMLEEPITYGEMY 290
           +LSNGRFKN DHQ V +SN+SR+SIA+F NP P+A VYPLKV E EK +LEEPIT+ EMY
Sbjct: 181 FLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMY 240

Query: 291 TRKMNKEIPL 301
            RKM +++ L
Sbjct: 241 KRKMGRDLEL 249

[Close]

BLAST of MC07g0265 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 2.7e-41
Identity = 98/303 (32.34%), Postives = 170/303 (56.11%), Query Frame = 0


Query: 2   EIPIISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFDM 61
           + P+I L   D      R  + ++I +AC  +G FQV++HGV+ +++ +M+ +A++FF M
Sbjct: 37  DFPLIDLSSTD------RSFLIQQIHQACARFGFFQVINHGVNKQIIDEMVSVAREFFSM 96

Query: 62  SSQEKLRFDMSSGKRGGFLATT-HLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWA 121
           S +EK++       +   L+T+ ++K+E     R+ +    YPI ++  + WP  P  + 
Sbjct: 97  SMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLRLHCYPI-HKYVNEWPSNPPSFK 156

Query: 122 ATTAEYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVG 181
              ++Y  ++ ++  K+ E++SE++ +EK+ +KK   E  + + VN+YP C +  ++  G
Sbjct: 157 EIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQHMAVNYYPPCPEP-ELTYG 216

Query: 182 LQRRSDPGTITILLQD-HVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKN 241
           L   +DP  +TILLQD  VCGLQ   DG   W  V P   AFV+NIGD    LSNG +K+
Sbjct: 217 LPAHTDPNALTILLQDTTVCGLQILIDG--QWFAVNPHPDAFVINIGDQLQALSNGVYKS 276

Query: 242 GDHQVVASSNTSRVSIASFHNPKPEAIVYPLK----VSEDEKPMLEEPITYGEMYTRKMN 299
             H+ V ++   R+S+ASF  P   A++ P K      +DE   + +  TY E Y +  +
Sbjct: 277 VWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWS 329

[Close]

BLAST of MC07g0265 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 5.1e-40
Identity = 91/291 (31.27%), Postives = 155/291 (53.26%), Query Frame = 0


Query: 6   ISLVGIDDEVNGRRGDIKKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFFDMSSQE 65
           I L+ + D     R DI  + A AC   G FQ+ +HGV  + +  M++ A++FF  S  E
Sbjct: 42  IPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQSESE 101

Query: 66  KLRFDMSSGKRGGFLATT-HLKEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWAATTA 125
           +++   +  K+   L+T+ ++ +E     R+ +    YPIE+   + WP  P  +   TA
Sbjct: 102 RVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIED-FINEWPSTPISFREVTA 161

Query: 126 EYGEKMMDLACKLLEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVGLQRR 185
           EY   +  L   LLE +SE++ + K+ +     +  + + +N+YP+C    ++  GL   
Sbjct: 162 EYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRC-PQPELTYGLPGH 221

Query: 186 SDPGTITILLQDHVCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNGDHQV 245
            D   IT+LLQD V GLQ  KDG   W+ V P+   F+VN+GD    +SN ++K+  H+ 
Sbjct: 222 KDANLITVLLQDEVSGLQVFKDG--KWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRA 281

Query: 246 VASSNTSRVSIASFHNPKPEAIVYP---LKVSEDEKPMLEEPITYGEMYTR 293
           V +S+  R+SI +F+ P  +A++ P   L   E++ P +    TY E + +
Sbjct: 282 VVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEYFEK 328

[Close]

BLAST of MC07g0265 vs. TAIR 10
Match: AT4G16330.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 6.7e-40
Identity = 96/271 (35.42%), Postives = 152/271 (56.09%), Query Frame = 0


Query: 23  KKKIAEACEVWGIFQVVDHGVDAKLVSDMIHLAKDFF-DMSSQEKLRF--DMSSGKRGGF 82
           ++ I +AC  WG F V++HGV   L+  M  L   FF D   +EKLR+  D +S    G+
Sbjct: 79  REAIGDACRDWGAFHVINHGVPIHLLDRMRSLGLSFFQDSPMEEKLRYACDSTSAASEGY 138

Query: 83  LATTHL--KEEAKRYSREIVTFFTYPIENRDYSLWPDKPEGWAATTAEYGEKMMDLACKL 142
            +   L  K++     R+     T+P   R+ S WP  P  +     EYG++M  LA  L
Sbjct: 139 GSRMLLGAKDDVVLDWRDYFDHHTFPPSRRNPSHWPIHPSDYRQVVGEYGDEMKKLAQML 198

Query: 143 LEVLSEAMRMEKEAIKKACLEMYKKILVNFYPKCGDSNDVVVGLQRRSDPGTITILLQDH 202
           L ++SE++ +   +I++A  E+Y+ I V +YP C    ++ +GLQ  SD G IT+L+QD 
Sbjct: 199 LGLISESLGLPCSSIEEAVGEIYQNITVTYYPPC-PQPELTLGLQSHSDFGAITLLIQDD 258

Query: 203 VCGLQATKDGGKSWVDVAPLKGAFVVNIGDHGHYLSNGRFKNGDHQVVASSNTSRVSIAS 262
           V GLQ  KD    W+ V P+  A ++ I D    ++NGR+K+  H+ V ++N +R+S+A+
Sbjct: 259 VEGLQLYKDA--QWLTVPPISDAILILIADQTEIITNGRYKSAQHRAVTNANRARLSVAT 318

Query: 263 FHNPKPEAIVYPLKVSEDEKPMLEEPITYGE 289
           FH+P   A + P  VS+   P  +E + YG+
Sbjct: 319 FHDPSKTARIAP--VSQLSPPSYKE-VVYGQ 343

The following BLAST results are available for this feature:

BLAST of MC07g0265 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05965	1.4e-114	64.67	Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 G...	[more]
Q9S818	1.8e-114	64.33	Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H P...	[more]
Q07353	5.2e-114	64.33	Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN...	[more]
A0A4D6Q4T7	4.4e-113	63.67	Flavanone 3-dioxygenase F3H2 OS=Crocosmia x crocosmiiflora OX=1053288 GN=F3H-2 P...	[more]
A0A4D6Q9B0	2.2e-112	63.33	Flavanone 3-dioxygenase F3H1 OS=Crocosmia x crocosmiiflora OX=1053288 GN=F3H-1 P...	[more]

BLAST of MC07g0265 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156653.1	1.29e-220	100.00	naringenin,2-oxoglutarate 3-dioxygenase-like [Momordica charantia]	[more]
XP_022158003.1	4.17e-178	81.33	naringenin,2-oxoglutarate 3-dioxygenase-like [Momordica charantia]	[more]
KAG7028205.1	1.99e-148	67.67	Naringenin,2-oxoglutarate 3-dioxygenase, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_023539914.1	2.72e-148	67.67	naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita pepo subsp. pepo]	[more]
XP_022939196.1	3.73e-148	67.67	naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita moschata]	[more]

BLAST of MC07g0265 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DR74	6.25e-221	100.00	naringenin,2-oxoglutarate 3-dioxygenase-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1DUW5	2.02e-178	81.33	naringenin,2-oxoglutarate 3-dioxygenase-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1FF87	1.80e-148	67.67	naringenin,2-oxoglutarate 3-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KVH7	3.05e-148	68.00	naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6P8DVK5	1.88e-147	67.67	naringenin,2-oxoglutarate 3-dioxygenase OS=Punica granatum OX=22663 GN=LOC116211...	[more]

BLAST of MC07g0265 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51240.1	1.3e-115	64.33	flavanone 3-hydroxylase	[more]
AT3G51240.2	2.8e-94	63.20	flavanone 3-hydroxylase	[more]
AT5G24530.1	2.7e-41	32.34	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	5.1e-40	31.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G16330.2	6.7e-40	35.42	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 164..261 e-value: 6.7E-18 score: 65.0
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..298 e-value: 9.6E-76 score: 257.2
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 3..114 e-value: 9.9E-17 score: 61.8
None	No IPR available	PANTHER	PTHR47990:SF11	NARINGENIN,2-OXOGLUTARATE 3-DIOXYGENASE	coord: 1..298
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 1..298
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 2..296
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 159..261 score: 9.775475

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0265.1	MC07g0265.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901576	organic substance biosynthetic process
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen