MC07g0168 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC07g0168
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	rRNA N-glycosidase
Location	MC07: 3355126 .. 3355932 (+)
RNA-Seq Expression	MC07g0168
Synteny	MC07g0168

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGAAATTGTTAGTGTTTTCTTTGCTAATTCTTACCATCTTCCTTGCATCTCCTACCGTCGAGGGCGATGTGAGCTTCAACTTGTCCACGGCCACTAAAACATCCTATGGAGCTTTCCTCAATGCTTTTAGAGCTGATCTGCCACTTGAAAATAAGAAACCCTACAATATATCTCTACTGCGTGCCACGGTTGCAGGCGCAGCTCGCTACACACGAATCCATCTTACCGACCGCAGTGGTCGTGCCATCACGCTGGCTATAGATGTAACAAACGTTTACCTTCTCGGGTTTTACTCCCCGCAGTGTAATATATCCCACTTCTTCAATGACCCTGTTTCCATTGCAGCTAAGAGCTTCGTATTCACCAATTCTAAGGAGCAAAAAACCCTTCCATTTGATGGCAGTTACCCACAACTTGCTGGGGTTGGAGCCAACCGAAGCTCCCTTGTACTTGGCCTCCCATCCCTCAACAATTTCATTCCAACGTTGGCGGGCTACAACTGCCCCAACACCAACTACCAGCCCGCCGATGTCGCGAAGGCGTGTGTGGTAACCATTCAGACGATGTCGGAGGCAGCAAGATTTCAAAAGATTCAGGATGCAGTTCCAAACAATTTGGTACCAAATGCAGAAATTCTATCTTTAGAAAACAATTGGGGGAAACTCTCCAAACAAGTTCAGCTAGCAGAGAGTAATGGTGGACGATTTAAAAGTAACGTCACGCTTCAAGACCCGACCGGTGCTACGGTTATTGTGTCAAACGTTAATTCACCATATGTAAGAGGCAACATTCGGTTGTTGCTG

mRNA sequence

ATGAGGAAATTGTTAGTGTTTTCTTTGCTAATTCTTACCATCTTCCTTGCATCTCCTACCGTCGAGGGCGATGTGAGCTTCAACTTGTCCACGGCCACTAAAACATCCTATGGAGCTTTCCTCAATGCTTTTAGAGCTGATCTGCCACTTGAAAATAAGAAACCCTACAATATATCTCTACTGCGTGCCACGGTTGCAGGCGCAGCTCGCTACACACGAATCCATCTTACCGACCGCAGTGGTCGTGCCATCACGCTGGCTATAGATGTAACAAACGTTTACCTTCTCGGGTTTTACTCCCCGCAGTGTAATATATCCCACTTCTTCAATGACCCTGTTTCCATTGCAGCTAAGAGCTTCGTATTCACCAATTCTAAGGAGCAAAAAACCCTTCCATTTGATGGCAGTTACCCACAACTTGCTGGGGTTGGAGCCAACCGAAGCTCCCTTGTACTTGGCCTCCCATCCCTCAACAATTTCATTCCAACGTTGGCGGGCTACAACTGCCCCAACACCAACTACCAGCCCGCCGATGTCGCGAAGGCGTGTGTGGTAACCATTCAGACGATGTCGGAGGCAGCAAGATTTCAAAAGATTCAGGATGCAGTTCCAAACAATTTGGTACCAAATGCAGAAATTCTATCTTTAGAAAACAATTGGGGGAAACTCTCCAAACAAGTTCAGCTAGCAGAGAGTAATGGTGGACGATTTAAAAGTAACGTCACGCTTCAAGACCCGACCGGTGCTACGGTTATTGTGTCAAACGTTAATTCACCATATGTAAGAGGCAACATTCGGTTGTTGCTG

Coding sequence (CDS)

ATGAGGAAATTGTTAGTGTTTTCTTTGCTAATTCTTACCATCTTCCTTGCATCTCCTACCGTCGAGGGCGATGTGAGCTTCAACTTGTCCACGGCCACTAAAACATCCTATGGAGCTTTCCTCAATGCTTTTAGAGCTGATCTGCCACTTGAAAATAAGAAACCCTACAATATATCTCTACTGCGTGCCACGGTTGCAGGCGCAGCTCGCTACACACGAATCCATCTTACCGACCGCAGTGGTCGTGCCATCACGCTGGCTATAGATGTAACAAACGTTTACCTTCTCGGGTTTTACTCCCCGCAGTGTAATATATCCCACTTCTTCAATGACCCTGTTTCCATTGCAGCTAAGAGCTTCGTATTCACCAATTCTAAGGAGCAAAAAACCCTTCCATTTGATGGCAGTTACCCACAACTTGCTGGGGTTGGAGCCAACCGAAGCTCCCTTGTACTTGGCCTCCCATCCCTCAACAATTTCATTCCAACGTTGGCGGGCTACAACTGCCCCAACACCAACTACCAGCCCGCCGATGTCGCGAAGGCGTGTGTGGTAACCATTCAGACGATGTCGGAGGCAGCAAGATTTCAAAAGATTCAGGATGCAGTTCCAAACAATTTGGTACCAAATGCAGAAATTCTATCTTTAGAAAACAATTGGGGGAAACTCTCCAAACAAGTTCAGCTAGCAGAGAGTAATGGTGGACGATTTAAAAGTAACGTCACGCTTCAAGACCCGACCGGTGCTACGGTTATTGTGTCAAACGTTAATTCACCATATGTAAGAGGCAACATTCGGTTGTTGCTG

Protein sequence

MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISLLRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSFVFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNVTLQDPTGATVIVSNVNSPYVRGNIRLLL

Homology

[Close]

BLAST of MC07g0168 vs. ExPASy Swiss-Prot
Match: P33185 (Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3)

HSP 1 Score: 212.6 bits (540), Expect = 5.4e-54
Identity = 124/274 (45.26%), Postives = 177/274 (64.60%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           M KLLV  LLILTIFL SPTVEGDVSF LS AT TSYG F+   R  LP E +K YNI L
Sbjct: 1   MIKLLVLWLLILTIFLKSPTVEGDVSFRLSGATTTSYGVFIKNLREALPYE-RKVYNIPL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           LR++++G+ RYT +HLT+ +   I++A+DVTNVY++G+ +   ++S+FFN+  +  A  F
Sbjct: 61  LRSSISGSGRYTLLHLTNYADETISVAVDVTNVYIMGYLAG--DVSYFFNEASATEAAKF 120

Query: 121 VFTNSKEQKTLPFDGSYPQL-AGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADV 180
           VF ++K++ TLP+ G+Y +L    G  R ++ LGLP+L++ I TL  Y         +  
Sbjct: 121 VFKDAKKKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLYYYTA-------SSA 180

Query: 181 AKACVVTIQTMSEAARF----QKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGG 240
           A A +V IQ+ +E+AR+    Q+I   V    +P+   +SLENNW  LSKQ+Q+A +N G
Sbjct: 181 ASALLVLIQSTAESARYKFIEQQIGKRVDKTFLPSLATISLENNWSALSKQIQIASTNNG 240

Query: 241 RFKSNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 270
           +F+S V L D     V ++N ++  V  NI LLL
Sbjct: 241 QFESPVVLIDGNNQRVSITNASARVVTSNIALLL 264

[Close]

BLAST of MC07g0168 vs. ExPASy Swiss-Prot
Match: P24478 (Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 205.7 bits (522), Expect = 6.6e-52
Identity = 122/274 (44.53%), Postives = 177/274 (64.60%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           M + LVFSLLILT+FL +P VEGDVSF LS AT +SYG F++  R  LP E +K Y+I L
Sbjct: 1   MIRFLVFSLLILTLFLTAPAVEGDVSFRLSGATSSSYGVFISNLRKALPYE-RKLYDIPL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           LR+T+ G+ RY  IHLT+ +   I++AIDVTNVY++G+ +   + S+FFN+  +  A  +
Sbjct: 61  LRSTLPGSQRYALIHLTNYADETISVAIDVTNVYVMGYRAG--DTSYFFNEASATEAAKY 120

Query: 121 VFTNSKEQKTLPFDGSYPQL-AGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADV 180
           VF ++K + TLP+ G+Y +L    G  R ++ LGLP+L++ I TL  YN  +        
Sbjct: 121 VFKDAKRKVTLPYSGNYERLQIAAGKIRENIPLGLPALDSAITTLFYYNANS-------A 180

Query: 181 AKACVVTIQTMSEAARF----QKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGG 240
           A A +V IQ+ SEAAR+    Q+I   V    +P+  I+SLEN+W  LSKQ+Q+A +N G
Sbjct: 181 ASALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNG 240

Query: 241 RFKSNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 270
           +F++ V L +     V ++NV++  V  NI LLL
Sbjct: 241 QFETPVVLINAQNQRVTITNVDAGVVTSNIALLL 264

[Close]

BLAST of MC07g0168 vs. ExPASy Swiss-Prot
Match: P09989 (Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=3677 PE=1 SV=2)

HSP 1 Score: 200.7 bits (509), Expect = 2.1e-50
Identity = 120/274 (43.80%), Postives = 175/274 (63.87%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           M + LV SLLILT+FL +P VEGDVSF LS AT +SYG F++  R  LP E +K Y+I L
Sbjct: 1   MIRFLVLSLLILTLFLTTPAVEGDVSFRLSGATSSSYGVFISNLRKALPNE-RKLYDIPL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           LR+++ G+ RY  IHLT+ +   I++AIDVTNVY++G+ +   + S+FFN+  +  A  +
Sbjct: 61  LRSSLPGSQRYALIHLTNYADETISVAIDVTNVYIMGYRAG--DTSYFFNEASATEAAKY 120

Query: 121 VFTNSKEQKTLPFDGSYPQL-AGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADV 180
           VF ++  + TLP+ G+Y +L    G  R ++ LGLP+L++ I TL  YN  +        
Sbjct: 121 VFKDAMRKVTLPYSGNYERLQTAAGKIRENIPLGLPALDSAITTLFYYNANS-------A 180

Query: 181 AKACVVTIQTMSEAARF----QKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGG 240
           A A +V IQ+ SEAAR+    Q+I   V    +P+  I+SLEN+W  LSKQ+Q+A +N G
Sbjct: 181 ASALMVLIQSTSEAARYKFIEQQIGKRVDKTFLPSLAIISLENSWSALSKQIQIASTNNG 240

Query: 241 RFKSNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 270
           +F+S V L +     V ++NV++  V  NI LLL
Sbjct: 241 QFESPVVLINAQNQRVTITNVDAGVVTSNIALLL 264

[Close]

BLAST of MC07g0168 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 193.7 bits (491), Expect = 2.6e-48
Identity = 117/274 (42.70%), Postives = 165/274 (60.22%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           M +  V S LIL IFL    V+GDVSF LS A   SYG F+   R  LP   +K YNI L
Sbjct: 1   MSRFSVLSFLILAIFLGGSIVKGDVSFRLSGADPRSYGMFIKDLRNALPF-REKVYNIPL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           L  +V+GA RY  +HL +  G+ IT+A+DVTNVY++G+ +     S+FFN+P +  A  +
Sbjct: 61  LLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLAD--TTSYFFNEPAAELASQY 120

Query: 121 VFTNSKEQKTLPFDGSYPQL-AGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADV 180
           VF +++ + TLP+ G+Y +L    G  R  + +GLP+L++ I TL        +Y     
Sbjct: 121 VFRDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTL-------LHYDSTAA 180

Query: 181 AKACVVTIQTMSEAARF----QKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGG 240
           A A +V IQT +EAARF    Q+IQ+    + VP+   +SLEN+W  LSKQ+QLA+ N G
Sbjct: 181 AGALLVLIQTTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNG 240

Query: 241 RFKSNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 270
            F++ + L D  G  V ++NV S  V  NI+LLL
Sbjct: 241 IFRTPIVLVDNKGNRVQITNVTSKVVTSNIQLLL 264

[Close]

BLAST of MC07g0168 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 2.7e-45
Identity = 113/268 (42.16%), Postives = 165/268 (61.57%), Query Frame = 0


Query: 7   FSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISLLRATVA 66
           F++LIL IF+A+ TVE DV F+LS ++ TSY  F+   R  LP  N   YNI+LL ++ +
Sbjct: 4   FTVLILAIFVAASTVEADVRFSLSGSSSTSYSKFIGDLRKALP-SNGTVYNITLLLSSAS 63

Query: 67  GAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSFVFTNSK 126
           GA+RYT + L++  G+AIT+A+DVTNVY++G+     + S+FFN+  +  A  +VF  S 
Sbjct: 64  GASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVN--STSYFFNESDAKLASQYVFKGS- 123

Query: 127 EQKTLPFDGSYPQL-AGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKACVV 186
              TLP+ G+Y +L    G  R  + LG P+L++ I TL        +Y     A A +V
Sbjct: 124 TIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTL-------FHYDSTAAAAAFLV 183

Query: 187 TIQTMSEAARFQKIQ----DAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNV 246
            IQT +EA+RF+ I+    + +  N VP+   +SLEN W  LSKQ+QLA++N G FK+ V
Sbjct: 184 IIQTTAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPV 243

Query: 247 TLQDPTGATVIVSNVNSPYVRGNIRLLL 270
            + D  G  V ++NV S  V  NI+LLL
Sbjct: 244 VITDDKGQRVEITNVTSKVVTKNIQLLL 260

[Close]

BLAST of MC07g0168 vs. NCBI nr
Match: XP_022156706.1 (ribosome-inactivating protein bryodin I-like, partial [Momordica charantia])

HSP 1 Score: 527 bits (1358), Expect = 1.28e-187
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL
Sbjct: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF
Sbjct: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120

Query: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA 180
           VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA
Sbjct: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA 180

Query: 181 KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN 240
           KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN
Sbjct: 181 KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN 240

Query: 241 VTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           VTLQDPTGATVIVSNVNSPYVRGNIRLLL
Sbjct: 241 VTLQDPTGATVIVSNVNSPYVRGNIRLLL 269

[Close]

BLAST of MC07g0168 vs. NCBI nr
Match: XP_022158190.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 334 bits (856), Expect = 5.69e-112
Identity = 177/270 (65.56%), Postives = 209/270 (77.41%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           M KLLVF LLIL +FLA+PTVEGDVSFNLS  TKTSY  FLNA+RADLP + +  ++I L
Sbjct: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIIL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           L   V G++RYTRIHLT+   + I LAIDVTNVY+LGFY+ +CN+SHFFND V+  AK F
Sbjct: 61  LPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKF 120

Query: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNN-FIPTLAGYNCPNTNYQPADV 180
           VFT+SK+Q TL FDGSY QLAG G  R SL LGL  LN  +IPTL GYNCP  +Y+P+DV
Sbjct: 121 VFTDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180

Query: 181 AKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKS 240
           AKA VV IQT+SEAARFQKIQ  V  N +P AE+LSLENNW KLSK V+ A+   G FK 
Sbjct: 181 AKAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKV 240

Query: 241 NVTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           N TL++ TG  V+VSNVNSPYV+GNI+LLL
Sbjct: 241 NFTLENATGTPVVVSNVNSPYVKGNIQLLL 268

[Close]

BLAST of MC07g0168 vs. NCBI nr
Match: XP_022158194.1 (ribosome-inactivating protein bryodin I-like [Momordica charantia])

HSP 1 Score: 332 bits (850), Expect = 4.05e-111
Identity = 171/267 (64.04%), Postives = 204/267 (76.40%), Query Frame = 0


Query: 3   KLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISLLR 62
           KL+VF LLI+ IF A+P+VEGDVSF L   TK SYGAFL  FR D+P+E+K  + I LLR
Sbjct: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61

Query: 63  ATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSFVF 122
           ATV GA RYTRIHL+DR    ITLAIDVTNVY+LGFY+P+C++SHFFND  +I AK +VF
Sbjct: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121

Query: 123 TNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKA 182
           TNSK Q TLPFDGSY +L   G +R  L LGL ++N  IP L  Y+CPN+    +DVAKA
Sbjct: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKA 181

Query: 183 CVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNVT 242
           CVV IQT+SEAARFQ I++AV NN+ P  EILSLENNWG LSKQVQLA+ N GRFK+ V 
Sbjct: 182 CVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATVQ 241

Query: 243 LQDPTGATVIVSNVNSPYVRGNIRLLL 269
           L   TG  V V +VNSPYV+GN++LLL
Sbjct: 242 LIGETGNPVNVMDVNSPYVKGNLQLLL 268

[Close]

BLAST of MC07g0168 vs. NCBI nr
Match: XP_022159206.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 274 bits (700), Expect = 5.61e-90
Identity = 134/151 (88.74%), Postives = 146/151 (96.69%), Query Frame = 0


Query: 119 SFVFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPAD 178
           +FVFTNS++++  PFDGSYPQL+G GANRSSLVLGLPSLNN+IPTLAGYNCPNTNYQP+D
Sbjct: 2   AFVFTNSQQRRP-PFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSD 61

Query: 179 VAKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFK 238
           VAKACV+TIQTMSEAARFQKIQDAVPNNLVPN EILSLENNWG+LS+QVQLAESNGGRF 
Sbjct: 62  VAKACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFT 121

Query: 239 SNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           SNVTLQDPTGATV+VSNVNSPYVRGNIRLLL
Sbjct: 122 SNVTLQDPTGATVMVSNVNSPYVRGNIRLLL 151

[Close]

BLAST of MC07g0168 vs. NCBI nr
Match: XP_022158438.1 (ribosome-inactivating protein cucurmosin-like [Momordica charantia])

HSP 1 Score: 250 bits (639), Expect = 7.00e-79
Identity = 142/273 (52.01%), Postives = 176/273 (64.47%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPT--VEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNI 60
           MRKL VF LL L IFLA+PT  V+G     +ST    SY + LN FR  L  +NKK  NI
Sbjct: 1   MRKLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALR-DNKKVCNI 60

Query: 61  SLLRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQ--CNISHFFNDPVSIA 120
            LLRA V    R+  I +   +   I LAID+ NVY+LG+Y+P   C+  H+F+DPV++ 
Sbjct: 61  PLLRANVIDKNRFIEISIAS-TNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVR 120

Query: 121 AKSFVFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQP 180
           A  +VF N  +QK+LPFDG YP     GANR SL LGL SLN+ +P L  Y C  T Y  
Sbjct: 121 ATQYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQ 180

Query: 181 ADVAKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGR 240
            DVAKA VV +Q +SEAARF+ IQ AVPNN  PNAE+LSL+N+W  LS+ VQ A+   GR
Sbjct: 181 TDVAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGR 240

Query: 241 FKSNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           FK  VTL D  G  V V N++S YV GN++LLL
Sbjct: 241 FKDKVTLNDEKGHPVDVWNISSKYVSGNLKLLL 271

[Close]

BLAST of MC07g0168 vs. ExPASy TrEMBL
Match: A0A6J1DVR9 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1)

HSP 1 Score: 527 bits (1358), Expect = 6.21e-188
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL
Sbjct: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF
Sbjct: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120

Query: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA 180
           VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA
Sbjct: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVA 180

Query: 181 KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN 240
           KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN
Sbjct: 181 KACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSN 240

Query: 241 VTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           VTLQDPTGATVIVSNVNSPYVRGNIRLLL
Sbjct: 241 VTLQDPTGATVIVSNVNSPYVRGNIRLLL 269

[Close]

BLAST of MC07g0168 vs. ExPASy TrEMBL
Match: A0A6J1DV53 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1)

HSP 1 Score: 334 bits (856), Expect = 2.75e-112
Identity = 177/270 (65.56%), Postives = 209/270 (77.41%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISL 60
           M KLLVF LLIL +FLA+PTVEGDVSFNLS  TKTSY  FLNA+RADLP + +  ++I L
Sbjct: 1   MTKLLVFCLLILAVFLAAPTVEGDVSFNLSGVTKTSYATFLNAYRADLPYK-ETVFDIIL 60

Query: 61  LRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSF 120
           L   V G++RYTRIHLT+   + I LAIDVTNVY+LGFY+ +CN+SHFFND V+  AK F
Sbjct: 61  LPVNVTGSSRYTRIHLTNSYNQTIQLAIDVTNVYVLGFYTTECNLSHFFNDSVATEAKKF 120

Query: 121 VFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNN-FIPTLAGYNCPNTNYQPADV 180
           VFT+SK+Q TL FDGSY QLAG G  R SL LGL  LN  +IPTL GYNCP  +Y+P+DV
Sbjct: 121 VFTDSKQQ-TLSFDGSYGQLAGAGGRRQSLKLGLVDLNYVYIPTLYGYNCPGYSYKPSDV 180

Query: 181 AKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKS 240
           AKA VV IQT+SEAARFQKIQ  V  N +P AE+LSLENNW KLSK V+ A+   G FK 
Sbjct: 181 AKAMVVVIQTISEAARFQKIQKDVKYNSIPTAEVLSLENNWSKLSKLVKQADPTTGNFKV 240

Query: 241 NVTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           N TL++ TG  V+VSNVNSPYV+GNI+LLL
Sbjct: 241 NFTLENATGTPVVVSNVNSPYVKGNIQLLL 268

[Close]

BLAST of MC07g0168 vs. ExPASy TrEMBL
Match: A0A6J1DYP1 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1)

HSP 1 Score: 332 bits (850), Expect = 1.96e-111
Identity = 171/267 (64.04%), Postives = 204/267 (76.40%), Query Frame = 0


Query: 3   KLLVFSLLILTIFLASPTVEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNISLLR 62
           KL+VF LLI+ IF A+P+VEGDVSF L   TK SYGAFL  FR D+P+E+K  + I LLR
Sbjct: 2   KLIVFCLLIIAIFHAAPSVEGDVSFTLKGVTKKSYGAFLKFFRGDVPVESKTVFGIPLLR 61

Query: 63  ATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQCNISHFFNDPVSIAAKSFVF 122
           ATV GA RYTRIHL+DR    ITLAIDVTNVY+LGFY+P+C++SHFFND  +I AK +VF
Sbjct: 62  ATVTGANRYTRIHLSDRDDHTITLAIDVTNVYVLGFYTPKCDVSHFFNDSAAIQAKKYVF 121

Query: 123 TNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPADVAKA 182
           TNSK Q TLPFDGSY +L   G +R  L LGL ++N  IP L  Y+CPN+    +DVAKA
Sbjct: 122 TNSKGQITLPFDGSYSKLQRAGGDREKLDLGLVAVNFNIPKLFDYSCPNSTQTLSDVAKA 181

Query: 183 CVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFKSNVT 242
           CVV IQT+SEAARFQ I++AV NN+ P  EILSLENNWG LSKQVQLA+ N GRFK+ V 
Sbjct: 182 CVVVIQTISEAARFQNIENAVQNNMPPTREILSLENNWGALSKQVQLADPNTGRFKATVQ 241

Query: 243 LQDPTGATVIVSNVNSPYVRGNIRLLL 269
           L   TG  V V +VNSPYV+GN++LLL
Sbjct: 242 LIGETGNPVNVMDVNSPYVKGNLQLLL 268

[Close]

BLAST of MC07g0168 vs. ExPASy TrEMBL
Match: A0A6J1E379 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111025625 PE=3 SV=1)

HSP 1 Score: 274 bits (700), Expect = 2.72e-90
Identity = 134/151 (88.74%), Postives = 146/151 (96.69%), Query Frame = 0


Query: 119 SFVFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQPAD 178
           +FVFTNS++++  PFDGSYPQL+G GANRSSLVLGLPSLNN+IPTLAGYNCPNTNYQP+D
Sbjct: 2   AFVFTNSQQRRP-PFDGSYPQLSGAGANRSSLVLGLPSLNNYIPTLAGYNCPNTNYQPSD 61

Query: 179 VAKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGRFK 238
           VAKACV+TIQTMSEAARFQKIQDAVPNNLVPN EILSLENNWG+LS+QVQLAESNGGRF 
Sbjct: 62  VAKACVITIQTMSEAARFQKIQDAVPNNLVPNPEILSLENNWGRLSRQVQLAESNGGRFT 121

Query: 239 SNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           SNVTLQDPTGATV+VSNVNSPYVRGNIRLLL
Sbjct: 122 SNVTLQDPTGATVMVSNVNSPYVRGNIRLLL 151

[Close]

BLAST of MC07g0168 vs. ExPASy TrEMBL
Match: A0A6J1DW33 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1)

HSP 1 Score: 250 bits (639), Expect = 3.39e-79
Identity = 142/273 (52.01%), Postives = 176/273 (64.47%), Query Frame = 0


Query: 1   MRKLLVFSLLILTIFLASPT--VEGDVSFNLSTATKTSYGAFLNAFRADLPLENKKPYNI 60
           MRKL VF LL L IFLA+PT  V+G     +ST    SY + LN FR  L  +NKK  NI
Sbjct: 1   MRKLFVFCLLFLAIFLAAPTYTVKGQRRITISTTNIDSYVSSLNTFRIALR-DNKKVCNI 60

Query: 61  SLLRATVAGAARYTRIHLTDRSGRAITLAIDVTNVYLLGFYSPQ--CNISHFFNDPVSIA 120
            LLRA V    R+  I +   +   I LAID+ NVY+LG+Y+P   C+  H+F+DPV++ 
Sbjct: 61  PLLRANVIDKNRFIEISIAS-TNITIGLAIDLVNVYVLGYYTPNPSCSAYHYFSDPVAVR 120

Query: 121 AKSFVFTNSKEQKTLPFDGSYPQLAGVGANRSSLVLGLPSLNNFIPTLAGYNCPNTNYQP 180
           A  +VF N  +QK+LPFDG YP     GANR SL LGL SLN+ +P L  Y C  T Y  
Sbjct: 121 ATQYVFKNPTQQKSLPFDGGYPDFEKYGANRRSLDLGLTSLNHLLPALYEYGCTGTKYIQ 180

Query: 181 ADVAKACVVTIQTMSEAARFQKIQDAVPNNLVPNAEILSLENNWGKLSKQVQLAESNGGR 240
            DVAKA VV +Q +SEAARF+ IQ AVPNN  PNAE+LSL+N+W  LS+ VQ A+   GR
Sbjct: 181 TDVAKALVVAVQVVSEAARFRMIQIAVPNNHKPNAEVLSLQNSWSDLSEAVQRADCGNGR 240

Query: 241 FKSNVTLQDPTGATVIVSNVNSPYVRGNIRLLL 269
           FK  VTL D  G  V V N++S YV GN++LLL
Sbjct: 241 FKDKVTLNDEKGHPVDVWNISSKYVSGNLKLLL 271

The following BLAST results are available for this feature:

BLAST of MC07g0168 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33185	5.4e-54	45.26	Ribosome-inactivating protein bryodin I OS=Bryonia dioica OX=3652 PE=1 SV=3	[more]
P24478	6.6e-52	44.53	Ribosome-inactivating protein karasurin-C OS=Trichosanthes kirilowii OX=3677 PE=...	[more]
P09989	2.1e-50	43.80	Ribosome-inactivating protein alpha-trichosanthin OS=Trichosanthes kirilowii OX=...	[more]
P16094	2.6e-48	42.70	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
Q00465	2.7e-45	42.16	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of MC07g0168 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156706.1	1.28e-187	100.00	ribosome-inactivating protein bryodin I-like, partial [Momordica charantia]	[more]
XP_022158190.1	5.69e-112	65.56	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022158194.1	4.05e-111	64.04	ribosome-inactivating protein bryodin I-like [Momordica charantia]	[more]
XP_022159206.1	5.61e-90	88.74	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]
XP_022158438.1	7.00e-79	52.01	ribosome-inactivating protein cucurmosin-like [Momordica charantia]	[more]

BLAST of MC07g0168 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVR9	6.21e-188	100.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111023550 PE=3 SV=1	[more]
A0A6J1DV53	2.75e-112	65.56	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024724 PE=3 SV=1	[more]
A0A6J1DYP1	1.96e-111	64.04	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024730 PE=3 SV=1	[more]
A0A6J1E379	2.72e-90	88.74	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111025625 PE=3 SV=1	[more]
A0A6J1DW33	3.39e-79	52.01	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024927 PE=3 SV=1	[more]

BLAST of MC07g0168 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 83..98 score: 48.68 coord: 35..48 score: 35.53 coord: 183..203 score: 46.12 coord: 211..228 score: 47.22 coord: 147..161 score: 37.89
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 21..194 e-value: 1.2E-38 score: 134.4
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 195..269 e-value: 6.3E-22 score: 79.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 33..228 e-value: 1.0E-29 score: 103.6
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 25..262
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 25..262
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 187..203
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 25..259

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC07g0168.1	MC07g0168.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity