MC07g0114 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0114
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionzf-RVT domain-containing protein
LocationMC07: 2190419 .. 2190837 (+)
RNA-Seq ExpressionMC07g0114
SyntenyMC07g0114
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCTACAAAGGAAGCTTCCTGGTTATATCCTTTTGCCCTCAATTTGCCCCCTTTGTCAATCCAATGAAGAGAGCTTGGATCACATCTTTTTCCTATGTCCCTTTGCTCACAGTGCTGGGAACTAATCTTTGAAGAGTTTAATCTGACTTGGGTGTTTGGAAACATAGATGCTGACAATGTCCTTCAGCTTCTTCTCGAGCCAGCCTTTAATCCTGAAGTCAACCTTCTTTGGCTCAATGCTATAAAGGCAGTACTTTATGAGTTGTGGTACAAAAGGAATCCCAAAGTTTTCGAAGAAAAGAGACAATCTCCAGAGATGCGTTTCAGTCTAGCTAAGTTTAAAGCTTCTCAATGGTGTTCCTTATCGTCCATCTTTAAAAGTTACACTCCTAGTTTGATATGTTCTAATTGGAAAGCT

mRNA sequence

ATCCTACAAAGGAAGCTTCCTGGTTATATCCTTTTGCCCTCAATTTGCCCCCTTTGTCAATCCAATGAAGAGAGCTTGGATCACATCTTTTTCCTATGTCCCTTTGCTCACTGCTGGGAACTAATCTTTGAAGAGTTTAATCTGACTTGGGTGTTTGGAAACATAGATGCTGACAATGTCCTTCAGCTTCTTCTCGAGCCAGCCTTTAATCCTGAAGTCAACCTTCTTTGGCTCAATGCTATAAAGGCAGTACTTTATGAGTTGTGGTACAAAAGGAATCCCAAAGTTTTCGAAGAAAAGAGACAATCTCCAGAGATGCGTTTCAGTCTAGCTAAGTTTAAAGCTTCTCAATGGTGTTCCTTATCGTCCATCTTTAAAAGTTACACTCCTAGTTTGATATGTTCTAATTGGAAAGCT

Coding sequence (CDS)

ATCCTACAAAGGAAGCTTCCTGGTTATATCCTTTTGCCCTCAATTTGCCCCCTTTGTCAATCCAATGAAGAGAGCTTGGATCACATCTTTTTCCTATGTCCCTTTGCTCACTGCTGGGAACTAATCTTTGAAGAGTTTAATCTGACTTGGGTGTTTGGAAACATAGATGCTGACAATGTCCTTCAGCTTCTTCTCGAGCCAGCCTTTAATCCTGAAGTCAACCTTCTTTGGCTCAATGCTATAAAGGCAGTACTTTATGAGTTGTGGTACAAAAGGAATCCCAAAGTTTTCGAAGAAAAGAGACAATCTCCAGAGATGCGTTTCAGTCTAGCTAAGTTTAAAGCTTCTCAATGGTGTTCCTTATCGTCCATCTTTAAAAGTTACACTCCTAGTTTGATATGTTCTAATTGGAAAGCT

Protein sequence

ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAHCWELIFEEFNLTWVFGNIDADNVLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWCSLSSIFKSYTPSLICSNWKA
Homology
BLAST of MC07g0114 vs. NCBI nr
Match: XP_022153214.1 (uncharacterized protein LOC111020765 [Momordica charantia])

HSP 1 Score: 140 bits (352), Expect = 8.81e-39
Identity = 70/141 (49.65%), Postives = 90/141 (63.83%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAH-CWELIFEEFNLTWVFGNIDADN 60
           I+Q+K P   LLPS C LC  + E  DH+FF C FA  CW L+F +FN+ W F     DN
Sbjct: 58  IIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYFASKCWNLLFHQFNVDWCFDLKAGDN 117

Query: 61  VLQLLLEPA-FNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQW 120
           V QLL  P   +  V  LWLN +KA+L ELW++RN ++FEEKR+  +  F  AKFKAS W
Sbjct: 118 VYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERNSRLFEEKRRLFDESFYSAKFKASLW 177

Query: 121 CSLSSIFKSYTPSLICSNWKA 139
           CSL   F  ++PS+I +NW A
Sbjct: 178 CSLVDSFLHHSPSMIYANWGA 198

BLAST of MC07g0114 vs. NCBI nr
Match: XP_038903695.1 (uncharacterized protein LOC120090219 [Benincasa hispida])

HSP 1 Score: 114 bits (286), Expect = 1.07e-27
Identity = 56/140 (40.00%), Postives = 80/140 (57.14%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAH-CWELIFEEFNLTWVFGNIDADN 60
           +LQ+K P   L P++CP C  + E   H+FF CP++  CW  +   FNL     N    N
Sbjct: 173 VLQKKQPTPSLSPTVCPFCLHHSEVSLHLFFTCPYSSWCWNKLLCFFNLPLTLCNDFKSN 232

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWC 120
           V QLL  P  +    LLW NA+KA+L +LW++RN ++F  K  S + R   A+ +AS WC
Sbjct: 233 VFQLLARPTSHKSTRLLWCNAVKALLADLWFERNQRIFYNKATSCQDRLEAARRQASSWC 292

Query: 121 SLSSIFKSYTPSLICSNWKA 139
            LS  F++Y+ S    NW+A
Sbjct: 293 LLSDPFRAYSLSDFNLNWEA 312

BLAST of MC07g0114 vs. NCBI nr
Match: KAA0035739.1 (hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa])

HSP 1 Score: 100 bits (250), Expect = 7.24e-22
Identity = 54/140 (38.57%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAHC-WELIFEEFNLTWVFGNIDADN 60
           ILQ+K P  +  PSICPLC    ++L HIF  CP +   WE IF  FNL W F +  + +
Sbjct: 243 ILQKKSPINVS-PSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSAS 302

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWC 120
           V+QLL          ++W    KA+L E+W +RN ++F +K +        A   A+ WC
Sbjct: 303 VIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWC 362

Query: 121 SLSSIFKSYTPSLICSNWKA 139
           SL   F +Y+   IC NW A
Sbjct: 363 SLRKEFVNYSIQDICLNWNA 381

BLAST of MC07g0114 vs. NCBI nr
Match: TYK21876.1 (hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 99.4 bits (246), Expect = 1.66e-21
Identity = 53/139 (38.13%), Postives = 74/139 (53.24%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAHC-WELIFEEFNLTWVFGNIDADN 60
           ILQ+K P  +  PSICPLC    ++L HIF  CP +   WE IF  FNL W F +  + +
Sbjct: 243 ILQKKSPINVS-PSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSAS 302

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWC 120
           V+QLL          ++W    KA+L E+W +RN ++F +K +        A   A+ WC
Sbjct: 303 VIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWC 362

Query: 121 SLSSIFKSYTPSLICSNWK 138
           SL   F +Y+   IC NW 
Sbjct: 363 SLRKEFVNYSIQDICLNWN 380

BLAST of MC07g0114 vs. NCBI nr
Match: KAA0062564.1 (GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 90.9 bits (224), Expect = 4.28e-18
Identity = 46/140 (32.86%), Postives = 71/140 (50.71%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFA-HCWELIFEEFNLTWVFGNIDADN 60
           ++QR+L    L+PS C LC    E L  +F  CP++  CWE +   F + W F      N
Sbjct: 558 VMQRELLNSCLMPSACSLCLEEGEDLQPLF-TCPYSVKCWENLLSLFGVDWAFDGSFNSN 617

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWC 120
           + Q+LL  +      L+W N  KA+L ++W++ N ++F  K      R  +AK  A+ WC
Sbjct: 618 IKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMHWSERLDIAKRNAATWC 677

Query: 121 SLSSIFKSYTPSLICSNWKA 139
            L+  F  Y+   +  NW A
Sbjct: 678 MLNKEFIDYSIQDLSVNWLA 696

BLAST of MC07g0114 vs. ExPASy TrEMBL
Match: A0A6J1DIE2 (uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020765 PE=4 SV=1)

HSP 1 Score: 140 bits (352), Expect = 4.26e-39
Identity = 70/141 (49.65%), Postives = 90/141 (63.83%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAH-CWELIFEEFNLTWVFGNIDADN 60
           I+Q+K P   LLPS C LC  + E  DH+FF C FA  CW L+F +FN+ W F     DN
Sbjct: 58  IIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYFASKCWNLLFHQFNVDWCFDLKAGDN 117

Query: 61  VLQLLLEPA-FNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQW 120
           V QLL  P   +  V  LWLN +KA+L ELW++RN ++FEEKR+  +  F  AKFKAS W
Sbjct: 118 VYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERNSRLFEEKRRLFDESFYSAKFKASLW 177

Query: 121 CSLSSIFKSYTPSLICSNWKA 139
           CSL   F  ++PS+I +NW A
Sbjct: 178 CSLVDSFLHHSPSMIYANWGA 198

BLAST of MC07g0114 vs. ExPASy TrEMBL
Match: A0A5A7T2Y0 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00100 PE=4 SV=1)

HSP 1 Score: 100 bits (250), Expect = 3.50e-22
Identity = 54/140 (38.57%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAHC-WELIFEEFNLTWVFGNIDADN 60
           ILQ+K P  +  PSICPLC    ++L HIF  CP +   WE IF  FNL W F +  + +
Sbjct: 243 ILQKKSPINVS-PSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSAS 302

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWC 120
           V+QLL          ++W    KA+L E+W +RN ++F +K +        A   A+ WC
Sbjct: 303 VIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWC 362

Query: 121 SLSSIFKSYTPSLICSNWKA 139
           SL   F +Y+   IC NW A
Sbjct: 363 SLRKEFVNYSIQDICLNWNA 381

BLAST of MC07g0114 vs. ExPASy TrEMBL
Match: A0A5D3DE60 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold494G00090 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 8.04e-22
Identity = 53/139 (38.13%), Postives = 74/139 (53.24%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAHC-WELIFEEFNLTWVFGNIDADN 60
           ILQ+K P  +  PSICPLC    ++L HIF  CP +   WE IF  FNL W F +  + +
Sbjct: 243 ILQKKSPINVS-PSICPLCLKASKNLPHIFLYCPVSSFGWERIFSLFNLVWNFDSSLSAS 302

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWC 120
           V+QLL          ++W    KA+L E+W +RN ++F +K +        A   A+ WC
Sbjct: 303 VIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDKARQRAEIMHAADLNAAAWC 362

Query: 121 SLSSIFKSYTPSLICSNWK 138
           SL   F +Y+   IC NW 
Sbjct: 363 SLRKEFVNYSIQDICLNWN 380

BLAST of MC07g0114 vs. ExPASy TrEMBL
Match: A0A5A7V5N8 (GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00520 PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 2.07e-18
Identity = 46/140 (32.86%), Postives = 71/140 (50.71%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFA-HCWELIFEEFNLTWVFGNIDADN 60
           ++QR+L    L+PS C LC    E L  +F  CP++  CWE +   F + W F      N
Sbjct: 558 VMQRELLNSCLMPSACSLCLEEGEDLQPLF-TCPYSVKCWENLLSLFGVDWAFDGSFNSN 617

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWC 120
           + Q+LL  +      L+W N  KA+L ++W++ N ++F  K      R  +AK  A+ WC
Sbjct: 618 IKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMHWSERLDIAKRNAATWC 677

Query: 121 SLSSIFKSYTPSLICSNWKA 139
            L+  F  Y+   +  NW A
Sbjct: 678 MLNKEFIDYSIQDLSVNWLA 696

BLAST of MC07g0114 vs. ExPASy TrEMBL
Match: A0A438JF01 (Putative mitochondrial protein OS=Vitis vinifera OX=29760 GN=AtMg01250_124 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 4.45e-15
Identity = 44/139 (31.65%), Postives = 69/139 (49.64%), Query Frame = 0

Query: 2   LQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFA-HCWELIFEEFNLTWVFGNIDADNV 61
           LQ + P   L P  C LC+ NEES+DH+F  CP     W  +F    L WV      D +
Sbjct: 359 LQLRRPYKSLCPQWCILCKGNEESIDHLFLHCPVTIGLWHKLFNLVGLVWVPPRSFEDML 418

Query: 62  LQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWCS 121
           +        +     LW  A   +++ +W +RN ++FE+K ++ EM + L +F +S W S
Sbjct: 419 VITFKGLGNSLRGKTLWQIACLTLIWMVWQERNNRIFEDKGRTEEMLWDLIRFYSSLWAS 478

Query: 122 LSSIFKSYTPSLICSNWKA 139
            +  F+    S++  NW A
Sbjct: 479 CTEAFRGVPLSVLQLNWIA 497

BLAST of MC07g0114 vs. TAIR 10
Match: AT1G43730.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 48.9 bits (115), Expect = 3.8e-06
Identity = 28/83 (33.73%), Postives = 41/83 (49.40%), Query Frame = 0

Query: 12  LPSICPLCQSNEESLDHIFFLCPFAHC-WELIFEEFNLTWVFGNIDADNVLQLLLEPAFN 71
           +P++C LC S++ES  H+FF CPF    W       NL   F        L  LL P+ +
Sbjct: 189 IPAVCLLCNSHDESRAHLFFECPFCGAVWRFFTGRANL---FPPAQLMYCLIWLLNPSRD 248

Query: 72  PEVNLLWLNAIKAVLYELWYKRN 94
               L+   A +A +Y +W +RN
Sbjct: 249 KNTTLIIRLAFQAYVYAIWRERN 268

BLAST of MC07g0114 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.1e-05
Identity = 35/122 (28.69%), Postives = 51/122 (41.80%), Query Frame = 0

Query: 16  CPLCQSNEESLDHIFFLCPFA-HCWELIFEEFNLTWVFGN---IDADNVLQLLLEPAFNP 75
           CP C    ES++H  F CPFA   W L         +  N    +  N+L  + +   + 
Sbjct: 200 CPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQDTTMSD 259

Query: 76  EVNLL--WLNAIKAVLYELWYKRNPKVFEEKRQSPEMRFSLAKFKASQWCSLSSIFKSYT 132
              LL  WL      ++ +W  RN  VF + R+SP      AK +   W + +   K  T
Sbjct: 260 FHKLLPVWL------IWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQSHKK-T 314

BLAST of MC07g0114 vs. TAIR 10
Match: AT1G45063.1 (copper ion binding;electron carriers )

HSP 1 Score: 45.4 bits (106), Expect = 4.2e-05
Identity = 29/101 (28.71%), Postives = 49/101 (48.51%), Query Frame = 0

Query: 1   ILQRKLPGYILLPSICPLCQSNEESLDHIFFLCPFAH-CWELIFEEFNLTWVFGNIDADN 60
           I  R +   I +PS+C LC + +E+  H+FF CPF+H  W        +T      D+  
Sbjct: 269 ICDRFISWGIQVPSLCLLCNALDETRQHVFFDCPFSHEVWSFFCSNARVTPPRMFKDS-- 328

Query: 61  VLQLLLEPAFNPEVNLLWLNAIKAVLYELWYKRNPKVFEEK 101
             + L  P  + +V  +   A +A +Y +W +RN ++   K
Sbjct: 329 -ARWLRHPCRDKKVAFILKLAYQASVYHIWRERNIRLNSNK 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022153214.18.81e-3949.65uncharacterized protein LOC111020765 [Momordica charantia][more]
XP_038903695.11.07e-2740.00uncharacterized protein LOC120090219 [Benincasa hispida][more]
KAA0035739.17.24e-2238.57hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa][more]
TYK21876.11.66e-2138.13hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa][more]
KAA0062564.14.28e-1832.86GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1DIE24.26e-3949.65uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A5A7T2Y03.50e-2238.57zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3DE608.04e-2238.13zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7V5N82.07e-1832.86GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A438JF014.45e-1531.65Putative mitochondrial protein OS=Vitis vinifera OX=29760 GN=AtMg01250_124 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G43730.13.8e-0633.73RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT3G09510.11.1e-0528.69Ribonuclease H-like superfamily protein [more]
AT1G45063.14.2e-0528.71copper ion binding;electron carriers [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0114.1MC07g0114.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
cellular_component GO:0005622 intracellular anatomical structure
molecular_function GO:0016787 hydrolase activity