MC07g0030 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC07g0030
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPyruvate dehydrogenase E1 component subunit beta
LocationMC07: 323477 .. 329387 (-)
RNA-Seq ExpressionMC07g0030
SyntenyMC07g0030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAACAATATCCGACTCCGTCGTCAATTTGGAACTTTGGGTTTTACTTGAGTGGACATGTTACACACGGTTGCTACTGTAACCATCGCCCATTCGAACCGCAGATTTTACATTGGTTTAATAGACAAGATCGTGAACGTTGGATCTGTTGACTAGTGAGAAGTCGTTGGTTCGGGTACACCATATTAACATGTGAGCTGCTCCACCAGAATGTAGACACACCGACTCTCCGTCTCCGTTGCCTCACAGTAGCAGTACTTGGCGGAATTATAATTATTAGAGAATAAAGGGTAATTAAGAAAATTAAAAAAGAATTCCAAAAGTAGAAATTCGAAAAAGAAAAATAAAAAATTTTAATTTTAGGAGAGTTTGGTAAGGTGGTGGTGGCCTGCCGTCTTCAAGTTCGAAGCCCTCCATTTCCCCAAGTCTCCCGTCTGATCGGATCCAACCCTTGCACTGATCTAAACTCCGGCCAGGTCTGGTTCTGATTATTCGCGTATTTTCAATTATTGTTCATGAATCTGATTTTGTTCTAGCTGCCGCTTTCTCCGTTGTGAAGCTTTACGATCTTTTTCAATTGCTTTTAGAATTCAGGTTTTCATTTCTGCTGCATTTGTTGTCTCTGTGACTGCATCTTCTGTATCTGATATTGGTTTTGATTTCTTTCCTCTATTGCTGAAGTAGAAAGTGAAACTGCTTGGAAATTGCGCGTTGGTGAATTTATGTTTGTGTGATATTGTAATGTTTGAAATAGAAGTACGATTTGGATCGTTTCAAATACGTGAGTGTTTGTTTCTGATTTCCGCAGAGCTATCTCTCGCGCGAGAAAAATGTGGGGAATCATTCGGCAAAAAGTCGGCGCTGGAGGCTCCTCCATTCTGGTCCGGTTCAATGCCTTTGGATGAAAAATTACTTTTTTCCATCACACATACGATTATTCTCTGAGGATTTTTATTCTTTCTTTTGTGTGTGTGCGTGTGTCTGTTTTTTTTTTTTTCCAGATTTCCGGGCAGTCTTTGCAGCGACTCCGCCCTGCTGCATCAGCATCGAGATACTATTCTTCTGCTGCAAAAGAGGTAATTCAGTGTCTTGTCTTGGGGCTTTCGGTACTTTACCTATTCTTATTCAGTCTTAGCTAGTGTTTTTCATAGTTTCAATATATCGTGTCCTTGTTACTCTTACTTTAGGATCAGGTTTGTTAGTGGCCATGCCTAGTTACTGTATGCTGAAATTTTTTAGAGTAAAATAATATGGTTTCTTGTCATCCAGTATGGTTAAGATTCCTTTTACTTTTGAAGAAATTTGAAAATCATGGGAGTGTTTTTTTTTAAAGAAATAAATTGTTCAGGACCATATTTTTCCTCATGTCATTGTCCAGCGAGTTATTCAGAAATGTATCTTTTAGATCTTCGTGCATAATGCAATTACCTTCCATTTACTTTCTTTTTCATATACAGTAGCATTATAATTTAGGAATGTTTGTCCCTGTTGCTCAATGTTGTTTAAAAGCAAAAGGCTAACTTTTTCACCCTAAATTTCATTCCAAATATGCACAGATGACAGTGAGAGATGCTCTAAACTCCGCACTTGATGAAGAAATGTCCGCTGACCCTAAAGTATTTTTGATGGGTGAAGAGGTGAGGCAGAATAAACATGAGCGTTTCTATTTTTATGTTAAATTGATGTGTCTATCCTTATGGCTTACTTCAAACATTCTAATGCAGGTTGGAGAATATCAGGGTGCATATAAGGTTAGATATGTCGAAGAGATGAGATCTAATACATGTGATTTTTTAATGTTGTATATGTTTATATTGTACTATTTAAATAATTGTTTGCCTTTATCTCCTCTCAAGTAGATAACCAAAGGGCTTTTGGACAAATATGGTCCAGAGAGGGTTCTTGATACCCCAATCACAGAGGTTGTTACTTCACCCCTGAAAATATATTTTTTCCGTCAATTTTATTGATCTAGTTTGAATTTTCACCTCTATGCCCGTGAAATATATGAAAGTAAAAGGATTATATTCTTTCTGACTTCAGTGTTCTCTAAATCTTTGCAGGCTGGGTTTACTGGTATCGGTGTTGGCGCTGCTTATCATGGTCTGAAGCCTGTTGTGGAGTTTATGACATTTAATTTCTCTATGCAGGTTATATGACTATTCTAAATACAACTCCATGTCATATTAGTGTTGTGTGCTTGTACTAGCTAGCTTTAAAAACTTTATATTGAAACTTTGTCCGTAAAAAACAACTTTGATGAATATGTTTGTTGTTGCCTTGTACTATGCTGCCTTATTTCATTAGCTGAGTGAAGTAAATTTTGACTCCAAATCATCTGGTAGCAGTGACGCTGACTATTGACTTTATTTGGTATTTCAGTTTTGGAACTGGAATAATTACATTGTTGTGCTTTCAATAGTTATAACTCACGTCCACTAACTTATGGAGGATCAATGAGAGAAAACCTTTGATAATATTGAACTCTTGGTGCAGTTTATTCCCCTATGGGTTGTGTGCTAATTTATGTTAGATCCTTGTGAGGGCTTTGACTAAATAAAGGAAAAAATGGAAAAAGAAAAGGAAAACTTGTGTTGAATAAGTGTCGTACCTAGCATTTTAAGTGCAATGTAGTTATAATAAATTCAGTATATCATATATTAAAATGTATTTGCTATATAGTTTACACTATTGATTCCTTCTATTGCTTTGCTTTCCACTTTTCTTGTCATCTGGAGTTTATCTTTCTTCGTATTAGAGTTGGCTTTCCAACTTAAATGTAACTATCCTTTAAGGATTTTGTTTGTATTAATTCTTCTGGTTCTATTCTCCATCTTTTCCCTTCACGAAGTTTGTCTTATTCACATTGAAGTTGCTTCTATCTCAGTTGGATAGTGGATTGTACCATCTTTTAGCTTTCTTAGGGCTCAGTAGTCATGATGCCATCATTACATCATTAATATAAATGTGCTAGTCTAATGTAGTTTTCCTTGTATTGATTCTTTCTACTTTTTTGTACTCCAACTCTCTTTTTTTCAATCACTGCTTGTCTCAGCTCCTTTCTCATTGCTGTAGGCAATTGATCATATCATTAATTCTGCGGCAAAAACAAACTATATGTCAGCTGGCCAAATTTCTGTGCCAATTGTCTTTAGAGGGCCTAATGGTGCGGCTGCTGGAGTTGGTGCCCAGCACTCTCAGGTTGATTTTTCTTTCTTCCCCTTTTATTCTTTTGCGGGTGCGTGTGAGCTTGTATATTCATATTATGTTTGTTTCTTGTATGTTGGGAAGCTTTTACGACCTAAAATTTTTAGACTTAACTTTAGATGGTGCAAGTAAGAAATCTTGAAATGCGCTTCAAATTTTCCTGTGCAGTGTTATGCAGCATGGTATGGCTCCTGTCCCGGCTTGAAAGTTTTGGCCCCATATTCATCTGAAGATGCTCGTGGACTTCTAAAAGCTGCCATAAGGGATCCAGATCCTGTGGTGTTTCTTGAAAATGAGTTATTGTAAGTAGTTAGTGAAAGTGTTTATATACATGCTAAGAAAATAATTAATAATTATGTGCACTGTTGCCTCAGATATGGTGAATCATTCCCTGTTTCGGCCGAGGTCCTTGATTCTAGTTTCACCGCTCCCATAGGAAAAGCTAAGGTGACTTTGTTGTTGCATTTTAATACATTTAAGAAGATAGTTTCTGTTTTTCTATCCTTCTTCTGTGTGTAAACTTTTTATCATTCATTTCTGACTTATGGTTTCCTAATGTTGATAGATTGAGAGAGAAGGGAAGGATGTGACTATTACTGCTTTTTCAAAGATGGTGGGCTACGCACTTAAGGTCAGTGTCTTGTATTGTGCAGTTATTTGGATCGTGGGGTTTGGTTGTGATAGGTATCTTGTATTTACTTTACTAAGAAGATCTTATAAAAAAAAACTTTACTAAGAGGAAAATAACAAGTTTACCACCTATCTCGCTCATGATCAGTTTGAAGGGTCATCTATCTCTCCTACATTACTTCAGACTCCACAAAAACAACACACGTGCCCAGACACAAGAACTACTTTATTTATAAGTAAAACAGAGCATAATTGACCAAGGTGTTATATCTTCCTCTTGAAACGTTTTCAAAACATAAAGGAACTAACTAAAACTGTTCACGGAAGTTATCAGGCTGAGACATTCTCCTTACCCTTTCTCTAATAAACTTTAATCACAAGAGGCCTAAAAGGTTCTTATTTTTAATTTTTTTAACTTAATTTGCTATGTCAAGGACTATGTCAATCTGTGAGGTAATGATAAGTTCTTTGGTGGGCCATGGGCGAGTGTGATTTGAAGTAGAACTCTCTTGAATATGTATCACTGGACTGGTTGGTGAGAAGCAATTATTTTAGTTGTGACATTTTTATTACGTTAGATATCAAGGAAAATTTATTCAGGTTCCAGAATATTCCTTATGCACTTGATGTAAACGGTGATAATTTTTCATAATGGTGCTCTACCGATTAAAAGTCTGGATCTGAAGATCGTACCCCAAGATGCAATATTGTTTTCTTGGCAGCAGCTTTTAGGTTGGAATAATGGGCAATTAAAATATACTTTCCTGGGTGGATATCATCATATCTTTGATACACATGTTTAGTGGAATTTATAGTTTCTAACGAGTTAAAAATTTCTCTCATCTCTCCATCCATTATTAATTCTTGCTTGGATTATCAGGCAGCTGAAATACTTTCCAAAGAAGGAATTAGTGCTGAGGTAATTTTTTCAGATGGAGTCTTCATCTTTTAGATGTTTGTACGCTGATTTTGTTTGGTTGATCAAAAGTGTAATAACCTATCTTGTCCATATAGGTTATAAACCTCCGCTCTATTCGCCCACTAGACAGAGCAACAATTAATGCTTCAGTGAGGAAGACAAGCAGATTAGTTACAGTTGAAGAAGGGTTCCCTCAGCATGGTGTTGGAGCTGAGATCTGGTTTTTTTCTCCCTCTCATGTTCACTCATCTTCTTGATTGATTTTGAAGACACCCTGTTAACTTACAGTGAATATTTCTTTTTATACAGCACATCTGTCGTCGAGGAAAGTTTTGGCTATCTTGATGCACCAGTGGAGAGGATTGCAGGGGCTGACATTCCCATGCCTTATGCAGCCAATCTCGAACGGATGGCTGTTCCTCAGGTTTATTTCCTTTCCTCATCCTGAAAATTTCTCTACTTTCAATAGCGAAAGAAAGAAGAAAGAAACTGTATATTGAGATTTATGTTGCTGATCTAAATTCCCGGTTTTCGCTGTTTGATGGTAATATTTGTATAGGTGGAGGACATTGTTCGAGCAGCAAAAAGAGCATGCTACAGAGCCGTACCATTGGCCGCAACTGGTTGATTTTGCCCTTCCAATCTGGAATTTTTTTTCCATCTTAGGGTAAGATTCAATGACTTCATTGTCCCTTTCAGTTTCTGTTCCCATTCCCTCATCCAAGAAGAAATTTCAGTCTTCCCTTGTACAATAAAAACCTTTTGATTTTAGCCGAGACATTTTACAACCATTCCTTCAACGGGCATGTCCAATCAAACTTGGAGATATTTTTATGAGCAAAAAAGAAAATCCATTTCTTGCCAAAATTAATCACAGAACATTTGACAGTGGTAAAATAATAAGTGGCTTAGCAGATGCAGGAGACTGTAGTGTACATTGTTTCTGTGATGGCGTCTGGTCTGTGAGTTGTTAAAATAACTTGGGAGAACATTGTCGAAGATGGGAATTTCATTTTTTGTCAACTCTATTCCATAGTCAGATAGATTATTCCTCTTCTGCTCTGCCCAAATTCAAACCTTTTGTTTGTCATGTAAGTTTCAATCTTCCCTTGTTCTTCGTTTTCCATTATGTCAACACCAAATCAGACCTACTGCCT

mRNA sequence

GAACAATATCCGACTCCGTCGTCAATTTGGAACTTTGGGTTTTACTTGAGTGGACATGTTACACACGGTTGCTACTGTAACCATCGCCCATTCGAACCGCAGATTTTACATTGGTTTAATAGACAAGATCGTGAACGTTGGATCTGTTGACTAGTGAGAAGTCGTTGGTTCGGGTACACCATATTAACATGTGAGCTGCTCCACCAGAATGTAGACACACCGACTCTCCGTCTCCGTTGCCTCACAGTAGCAGTACTTGGCGGAATTATAATTATTAGAGAATAAAGGGTAATTAAGAAAATTAAAAAAGAATTCCAAAAGTAGAAATTCGAAAAAGAAAAATAAAAAATTTTAATTTTAGGAGAGTTTGGTAAGGTGGTGGTGGCCTGCCGTCTTCAAGTTCGAAGCCCTCCATTTCCCCAAGTCTCCCGTCTGATCGGATCCAACCCTTGCACTGATCTAAACTCCGGCCAGAGCTATCTCTCGCGCGAGAAAAATGTGGGGAATCATTCGGCAAAAAGTCGGCGCTGGAGGCTCCTCCATTCTGATTTCCGGGCAGTCTTTGCAGCGACTCCGCCCTGCTGCATCAGCATCGAGATACTATTCTTCTGCTGCAAAAGAGATGACAGTGAGAGATGCTCTAAACTCCGCACTTGATGAAGAAATGTCCGCTGACCCTAAAGTATTTTTGATGGGTGAAGAGGTTGGAGAATATCAGGGTGCATATAAGATAACCAAAGGGCTTTTGGACAAATATGGTCCAGAGAGGGTTCTTGATACCCCAATCACAGAGGCTGGGTTTACTGGTATCGGTGTTGGCGCTGCTTATCATGGTCTGAAGCCTGTTGTGGAGTTTATGACATTTAATTTCTCTATGCAGGCAATTGATCATATCATTAATTCTGCGGCAAAAACAAACTATATGTCAGCTGGCCAAATTTCTGTGCCAATTGTCTTTAGAGGGCCTAATGGTGCGGCTGCTGGAGTTGGTGCCCAGCACTCTCAGTGTTATGCAGCATGGTATGGCTCCTGTCCCGGCTTGAAAGTTTTGGCCCCATATTCATCTGAAGATGCTCGTGGACTTCTAAAAGCTGCCATAAGGGATCCAGATCCTGTGGTGTTTCTTGAAAATGAGTTATTATATGGTGAATCATTCCCTGTTTCGGCCGAGGTCCTTGATTCTAGTTTCACCGCTCCCATAGGAAAAGCTAAGATTGAGAGAGAAGGGAAGGATGTGACTATTACTGCTTTTTCAAAGATGGTGGGCTACGCACTTAAGGCAGCTGAAATACTTTCCAAAGAAGGAATTAGTGCTGAGGTTATAAACCTCCGCTCTATTCGCCCACTAGACAGAGCAACAATTAATGCTTCAGTGAGGAAGACAAGCAGATTAGTTACAGTTGAAGAAGGGTTCCCTCAGCATGGTGTTGGAGCTGAGATCTGCACATCTGTCGTCGAGGAAAGTTTTGGCTATCTTGATGCACCAGTGGAGAGGATTGCAGGGGCTGACATTCCCATGCCTTATGCAGCCAATCTCGAACGGATGGCTGTTCCTCAGGTGGAGGACATTGTTCGAGCAGCAAAAAGAGCATGCTACAGAGCCGTACCATTGGCCGCAACTGGTTGATTTTGCCCTTCCAATCTGGAATTTTTTTTCCATCTTAGGGTAAGATTCAATGACTTCATTGTCCCTTTCAGTTTCTGTTCCCATTCCCTCATCCAAGAAGAAATTTCAGTCTTCCCTTGTACAATAAAAACCTTTTGATTTTAGCCGAGACATTTTACAACCATTCCTTCAACGGGCATGTCCAATCAAACTTGGAGATATTTTTATGAGCAAAAAAGAAAATCCATTTCTTGCCAAAATTAATCACAGAACATTTGACAGTGGTAAAATAATAAGTGGCTTAGCAGATGCAGGAGACTGTAGTGTACATTGTTTCTGTGATGGCGTCTGGTCTGTGAGTTGTTAAAATAACTTGGGAGAACATTGTCGAAGATGGGAATTTCATTTTTTGTCAACTCTATTCCATAGTCAGATAGATTATTCCTCTTCTGCTCTGCCCAAATTCAAACCTTTTGTTTGTCATGTAAGTTTCAATCTTCCCTTGTTCTTCGTTTTCCATTATGTCAACACCAAATCAGACCTACTGCCT

Coding sequence (CDS)

ATGTGGGGAATCATTCGGCAAAAAGTCGGCGCTGGAGGCTCCTCCATTCTGATTTCCGGGCAGTCTTTGCAGCGACTCCGCCCTGCTGCATCAGCATCGAGATACTATTCTTCTGCTGCAAAAGAGATGACAGTGAGAGATGCTCTAAACTCCGCACTTGATGAAGAAATGTCCGCTGACCCTAAAGTATTTTTGATGGGTGAAGAGGTTGGAGAATATCAGGGTGCATATAAGATAACCAAAGGGCTTTTGGACAAATATGGTCCAGAGAGGGTTCTTGATACCCCAATCACAGAGGCTGGGTTTACTGGTATCGGTGTTGGCGCTGCTTATCATGGTCTGAAGCCTGTTGTGGAGTTTATGACATTTAATTTCTCTATGCAGGCAATTGATCATATCATTAATTCTGCGGCAAAAACAAACTATATGTCAGCTGGCCAAATTTCTGTGCCAATTGTCTTTAGAGGGCCTAATGGTGCGGCTGCTGGAGTTGGTGCCCAGCACTCTCAGTGTTATGCAGCATGGTATGGCTCCTGTCCCGGCTTGAAAGTTTTGGCCCCATATTCATCTGAAGATGCTCGTGGACTTCTAAAAGCTGCCATAAGGGATCCAGATCCTGTGGTGTTTCTTGAAAATGAGTTATTATATGGTGAATCATTCCCTGTTTCGGCCGAGGTCCTTGATTCTAGTTTCACCGCTCCCATAGGAAAAGCTAAGATTGAGAGAGAAGGGAAGGATGTGACTATTACTGCTTTTTCAAAGATGGTGGGCTACGCACTTAAGGCAGCTGAAATACTTTCCAAAGAAGGAATTAGTGCTGAGGTTATAAACCTCCGCTCTATTCGCCCACTAGACAGAGCAACAATTAATGCTTCAGTGAGGAAGACAAGCAGATTAGTTACAGTTGAAGAAGGGTTCCCTCAGCATGGTGTTGGAGCTGAGATCTGCACATCTGTCGTCGAGGAAAGTTTTGGCTATCTTGATGCACCAGTGGAGAGGATTGCAGGGGCTGACATTCCCATGCCTTATGCAGCCAATCTCGAACGGATGGCTGTTCCTCAGGTGGAGGACATTGTTCGAGCAGCAAAAAGAGCATGCTACAGAGCCGTACCATTGGCCGCAACTGGTTGA

Protein sequence

MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSADPKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEFMTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCPGLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKIEREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVRAAKRACYRAVPLAATG
Homology
BLAST of MC07g0030 vs. ExPASy Swiss-Prot
Match: Q6Z1G7 (Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0536000 PE=2 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 4.3e-182
Identity = 331/377 (87.80%), Postives = 353/377 (93.63%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRP--AASASRYYSSAAKEMTVRDALNSALDEEMS 60
           M GI R+++G+G       GQ +Q LRP  AA+A+R YS+AAKEMTVR+ALNSALDEEMS
Sbjct: 1   MLGIARRRLGSG----CALGQLMQALRPAAAAAAARTYSAAAKEMTVREALNSALDEEMS 60

Query: 61  ADPKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVV 120
           ADP VFLMGEEVGEYQGAYKI+KGLLDKYGP+RVLDTPITEAGFTGIGVGAAY GL+PVV
Sbjct: 61  ADPSVFLMGEEVGEYQGAYKISKGLLDKYGPDRVLDTPITEAGFTGIGVGAAYQGLRPVV 120

Query: 121 EFMTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGS 180
           EFMTFNFSMQAIDHIINSAAK+NYMSAGQI+VPIVFRGPNGAAAGVGAQHSQCYAAWY  
Sbjct: 121 EFMTFNFSMQAIDHIINSAAKSNYMSAGQINVPIVFRGPNGAAAGVGAQHSQCYAAWYAH 180

Query: 181 CPGLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKA 240
            PGLKVL PYS+EDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSF  PIGKA
Sbjct: 181 VPGLKVLTPYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFCLPIGKA 240

Query: 241 KIEREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSR 300
           KIE+EGKDVTITAFSKMVGYAL+AAEILSKEGISAEVINLRSIRPLDRATINASVRKT+R
Sbjct: 241 KIEQEGKDVTITAFSKMVGYALQAAEILSKEGISAEVINLRSIRPLDRATINASVRKTNR 300

Query: 301 LVTVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDI 360
           LVT+EEGFPQHGVGAEIC SVVE+SF YLDAPVERIAGAD+PMPYAANLERMAVPQVEDI
Sbjct: 301 LVTLEEGFPQHGVGAEICMSVVEDSFEYLDAPVERIAGADVPMPYAANLERMAVPQVEDI 360

Query: 361 VRAAKRACYRAVPLAAT 376
           VRAAKRACYRAVP+AAT
Sbjct: 361 VRAAKRACYRAVPMAAT 373

BLAST of MC07g0030 vs. ExPASy Swiss-Prot
Match: Q0J0H4 (Pyruvate dehydrogenase E1 component subunit beta-2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0509200 PE=2 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.1e-180
Identity = 328/379 (86.54%), Postives = 351/379 (92.61%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRP----AASASRYYSSAAKEMTVRDALNSALDEE 60
           M G  R+++G+G     + GQ L+RLRP    AA A+R YS+AAKEMTVR+ALNSALDEE
Sbjct: 1   MLGAARRQLGSGP----MLGQVLRRLRPATAAAADAARAYSAAAKEMTVREALNSALDEE 60

Query: 61  MSADPKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKP 120
           MSADP VFLMGEEVGEYQGAYKI+KGLLDKYGPERVLDTPITEAGFTGI VGAAY GL+P
Sbjct: 61  MSADPSVFLMGEEVGEYQGAYKISKGLLDKYGPERVLDTPITEAGFTGIAVGAAYQGLRP 120

Query: 121 VVEFMTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWY 180
           VVEFMTFNFSMQAIDHIINSAAK+NYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWY
Sbjct: 121 VVEFMTFNFSMQAIDHIINSAAKSNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWY 180

Query: 181 GSCPGLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIG 240
              PGLKVL PYS+EDARGLLKAAIRDPDPVVFLENELLYGESFP+SAEVLDSSF  PIG
Sbjct: 181 AHVPGLKVLVPYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPISAEVLDSSFALPIG 240

Query: 241 KAKIEREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKT 300
           KAKIEREGKDVTITA+SKMVGYAL+AA+ILSKEGISAEVINLRSIRPLDRATINASVRKT
Sbjct: 241 KAKIEREGKDVTITAYSKMVGYALQAADILSKEGISAEVINLRSIRPLDRATINASVRKT 300

Query: 301 SRLVTVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVE 360
           +RLVT+EE FPQHG+GAEIC SVVEESF YLDAPVERIAGAD+PMPYAANLERMAVPQV+
Sbjct: 301 NRLVTIEESFPQHGIGAEICMSVVEESFEYLDAPVERIAGADVPMPYAANLERMAVPQVD 360

Query: 361 DIVRAAKRACYRAVPLAAT 376
           DIVRAAKRACYRAVP+AAT
Sbjct: 361 DIVRAAKRACYRAVPMAAT 375

BLAST of MC07g0030 vs. ExPASy Swiss-Prot
Match: P52904 (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 5.6e-174
Identity = 312/349 (89.40%), Postives = 331/349 (94.84%), Query Frame = 0

Query: 26  LRPAASASRYYSSAAKEMTVRDALNSALDEEMSADPKVFLMGEEVGEYQGAYKITKGLLD 85
           +RP+ SA R++SS AK+MTVRDALNSALD EMSAD KVFLMGEEVGEYQGAYK+TKGLL+
Sbjct: 10  IRPSFSAFRFFSS-AKQMTVRDALNSALDVEMSADSKVFLMGEEVGEYQGAYKVTKGLLE 69

Query: 86  KYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEFMTFNFSMQAIDHIINSAAKTNYMSA 145
           KYGPERVLDTPITEAGFTGIGVGAAY+GLKPVVEFMTFNFSMQAIDHIINSAAK+NYMSA
Sbjct: 70  KYGPERVLDTPITEAGFTGIGVGAAYYGLKPVVEFMTFNFSMQAIDHIINSAAKSNYMSA 129

Query: 146 GQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCPGLKVLAPYSSEDARGLLKAAIRDPD 205
           GQISVPIVFRG NG AAGVGAQHS CYA+WYGSCPGLKVL P+S+EDARGLLKAAIRDPD
Sbjct: 130 GQISVPIVFRGLNGDAAGVGAQHSHCYASWYGSCPGLKVLVPHSAEDARGLLKAAIRDPD 189

Query: 206 PVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKIEREGKDVTITAFSKMVGYALKAAEI 265
           PVVFLENELLYGESFPVSAEVLDSSF  PIGKAKIEREGKDVTITAFSKMVG+ALKAAEI
Sbjct: 190 PVVFLENELLYGESFPVSAEVLDSSFWLPIGKAKIEREGKDVTITAFSKMVGFALKAAEI 249

Query: 266 LSKEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVGAEICTSVVEESFG 325
           L KEGISAEVINLRSIRPLDR TINASVRKT+RLVTVEEGFPQHGVGAEICTSV+EESFG
Sbjct: 250 LEKEGISAEVINLRSIRPLDRPTINASVRKTNRLVTVEEGFPQHGVGAEICTSVIEESFG 309

Query: 326 YLDAPVERIAGADIPMPYAANLERMAVPQVEDIVRAAKRACYRAVPLAA 375
           YLDA VERI GAD+PMPYA NLER+ VP VEDIVRAAKRAC+R+VPLAA
Sbjct: 310 YLDATVERIGGADVPMPYAGNLERLVVPHVEDIVRAAKRACHRSVPLAA 357

BLAST of MC07g0030 vs. ExPASy Swiss-Prot
Match: Q38799 (Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PDH2 PE=1 SV=2)

HSP 1 Score: 607.1 bits (1564), Expect = 1.4e-172
Identity = 312/369 (84.55%), Postives = 337/369 (91.33%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           M GI+RQ+   G S       +L+R R A  ++R Y++ AKEMTVRDALNSA+DEEMSAD
Sbjct: 1   MLGILRQRAIDGAS-------TLRRTRFALVSARSYAAGAKEMTVRDALNSAIDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVF+MGEEVG+YQGAYKITKGLL+KYGPERV DTPITEAGFTGIGVGAAY GLKPVVEF
Sbjct: 61  PKVFVMGEEVGQYQGAYKITKGLLEKYGPERVYDTPITEAGFTGIGVGAAYAGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAK+NYMSAGQI+VPIVFRGPNGAAAGVGAQHSQCYAAWY S P
Sbjct: 121 MTFNFSMQAIDHIINSAAKSNYMSAGQINVPIVFRGPNGAAAGVGAQHSQCYAAWYASVP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYS+EDARGLLKAAIRDPDPVVFLENELLYGESFP+S E LDSSF  PIGKAKI
Sbjct: 181 GLKVLAPYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPISEEALDSSFCLPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTI  FSKMVG+ALKAAE L++EGISAEVINLRSIRPLDRATINASVRKTSRLV
Sbjct: 241 EREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGV AEIC SVVEESF YLDAPVERIAGAD+PMPYAANLER+A+PQ+EDIVR
Sbjct: 301 TVEEGFPQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVR 360

Query: 361 AAKRACYRA 370
           A+KRACYR+
Sbjct: 361 ASKRACYRS 362

BLAST of MC07g0030 vs. ExPASy Swiss-Prot
Match: Q86HX0 (Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=pdhB PE=1 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 2.2e-125
Identity = 220/330 (66.67%), Postives = 272/330 (82.42%), Query Frame = 0

Query: 39  AAKEMTVRDALNSALDEEMSADPKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPIT 98
           A KE+TVRDA+NSALDEE++ D KVF+MGEEV +Y GAYKITKGL DKYG +R++DTPIT
Sbjct: 25  ATKEVTVRDAINSALDEELARDEKVFIMGEEVAQYNGAYKITKGLFDKYGGDRIIDTPIT 84

Query: 99  EAGFTGIGVGAAYHGLKPVVEFMTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPN 158
           EAGF GIGVGAA  G +P++EFMTFNF+MQAIDHIINS+AKT+YMS G++  PIV+RGPN
Sbjct: 85  EAGFAGIGVGAAMAGTRPIIEFMTFNFAMQAIDHIINSSAKTHYMSGGKVFNPIVWRGPN 144

Query: 159 GAAAGVGAQHSQCYAAWYGSCPGLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGE 218
           G    VGAQHSQC+AAWYGS PGLKV+AP+S+ D RGLLK+AIRD +PVV+LE+ELLY  
Sbjct: 145 GPPTAVGAQHSQCFAAWYGSVPGLKVVAPWSAADHRGLLKSAIRDDNPVVYLESELLYNY 204

Query: 219 SFPVSAEVLDSSFTAPIGKAKIEREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINL 278
            F +S +  D  +  PIGKAK+EREGKDVTI  FS++V   ++AAEIL+KEGISAEVINL
Sbjct: 205 KFDLSDQEQDKEYLVPIGKAKVEREGKDVTIVGFSRIVSNCMEAAEILAKEGISAEVINL 264

Query: 279 RSIRPLDRATINASVRKTSRLVTVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGAD 338
           R+IRP+D  TI  S++KT++LVTVEEG+ Q G+GAEI   ++E +F YLDAP+ERI GAD
Sbjct: 265 RTIRPIDAETIVNSLKKTNKLVTVEEGWAQSGIGAEISALMMEHAFDYLDAPIERICGAD 324

Query: 339 IPMPYAANLERMAVPQVEDIVRAAKRACYR 369
           +PMPYA+NLE  A+ Q ++IV AAKR   R
Sbjct: 325 VPMPYASNLENAAMVQTQNIVNAAKRVTQR 354

BLAST of MC07g0030 vs. NCBI nr
Match: XP_022148931.1 (pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Momordica charantia])

HSP 1 Score: 726 bits (1874), Expect = 1.15e-263
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV
Sbjct: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR
Sbjct: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAATG
Sbjct: 361 AAKRACYRAVPLAATG 376

BLAST of MC07g0030 vs. NCBI nr
Match: XP_023548371.1 (pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023548372.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023548373.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023548374.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 711 bits (1834), Expect = 1.43e-257
Identity = 364/376 (96.81%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+R+KVGAG S IL SGQ+LQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIVRKKVGAGASCILTSGQALQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYG+CP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGACP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAE+LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 241 EREGKDVTITAFSKMVGYALKAAEVLSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDI+R
Sbjct: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIIR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAA+G
Sbjct: 361 AAKRACYRAVPLAASG 376

BLAST of MC07g0030 vs. NCBI nr
Match: XP_022953745.1 (pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita moschata] >XP_022953746.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita moschata] >XP_022953747.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita moschata] >XP_022953748.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 710 bits (1833), Expect = 2.04e-257
Identity = 363/376 (96.54%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+R+KVGAG S IL SGQ+LQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIVRKKVGAGASCILTSGQALQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYG+CP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGACP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAE+LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 241 EREGKDVTITAFSKMVGYALKAAEVLSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQ+EDI+R
Sbjct: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQIEDIIR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAA+G
Sbjct: 361 AAKRACYRAVPLAASG 376

BLAST of MC07g0030 vs. NCBI nr
Match: KAG6576110.1 (Pyruvate dehydrogenase E1 component subunit beta-2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 711 bits (1834), Expect = 5.12e-257
Identity = 364/376 (96.81%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+R+KVGAG S IL SGQ+LQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 35  MWGIVRKKVGAGASCILTSGQALQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 94

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 95  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 154

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYG+CP
Sbjct: 155 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGACP 214

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 215 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 274

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAE+LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 275 EREGKDVTITAFSKMVGYALKAAEVLSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 334

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDI+R
Sbjct: 335 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIIR 394

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAA+G
Sbjct: 395 AAKRACYRAVPLAASG 410

BLAST of MC07g0030 vs. NCBI nr
Match: XP_022991392.1 (pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial isoform X1 [Cucurbita maxima] >XP_022991393.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial isoform X1 [Cucurbita maxima] >XP_022991394.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial isoform X1 [Cucurbita maxima] >XP_022991396.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial isoform X1 [Cucurbita maxima] >XP_022991397.1 pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial isoform X2 [Cucurbita maxima])

HSP 1 Score: 708 bits (1827), Expect = 1.67e-256
Identity = 362/376 (96.28%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+R+KVGAG S IL SGQ+LQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIVRKKVGAGASCILTSGQALQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYG+CP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGACP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREG+DVTITAFSKMVGYALKAAE+LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 241 EREGEDVTITAFSKMVGYALKAAEVLSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPV+RIAGADIPMPYAANLERMAVPQVEDI+R
Sbjct: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVQRIAGADIPMPYAANLERMAVPQVEDIIR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAA+G
Sbjct: 361 AAKRACYRAVPLAASG 376

BLAST of MC07g0030 vs. ExPASy TrEMBL
Match: A0A6J1D6U9 (Pyruvate dehydrogenase E1 component subunit beta OS=Momordica charantia OX=3673 GN=LOC111017478 PE=4 SV=1)

HSP 1 Score: 726 bits (1874), Expect = 5.56e-264
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV
Sbjct: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR
Sbjct: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAATG
Sbjct: 361 AAKRACYRAVPLAATG 376

BLAST of MC07g0030 vs. ExPASy TrEMBL
Match: A0A6J1GNY3 (Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111456186 PE=4 SV=1)

HSP 1 Score: 710 bits (1833), Expect = 9.86e-258
Identity = 363/376 (96.54%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+R+KVGAG S IL SGQ+LQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIVRKKVGAGASCILTSGQALQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYG+CP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGACP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAE+LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 241 EREGKDVTITAFSKMVGYALKAAEVLSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQ+EDI+R
Sbjct: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQIEDIIR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAA+G
Sbjct: 361 AAKRACYRAVPLAASG 376

BLAST of MC07g0030 vs. ExPASy TrEMBL
Match: A0A6J1JUS0 (Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111488043 PE=4 SV=1)

HSP 1 Score: 708 bits (1827), Expect = 8.10e-257
Identity = 362/376 (96.28%), Postives = 373/376 (99.20%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+R+KVGAG S IL SGQ+LQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIVRKKVGAGASCILTSGQALQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYG+CP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGACP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREG+DVTITAFSKMVGYALKAAE+LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 241 EREGEDVTITAFSKMVGYALKAAEVLSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEICTSVVEESFGYLDAPV+RIAGADIPMPYAANLERMAVPQVEDI+R
Sbjct: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVQRIAGADIPMPYAANLERMAVPQVEDIIR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAA+G
Sbjct: 361 AAKRACYRAVPLAASG 376

BLAST of MC07g0030 vs. ExPASy TrEMBL
Match: A0A6J1KQ71 (Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111497694 PE=4 SV=1)

HSP 1 Score: 707 bits (1826), Expect = 1.15e-256
Identity = 364/376 (96.81%), Postives = 370/376 (98.40%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+RQK GA  SSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIVRQKAGAAASSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGF GIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFAGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAE LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 241 EREGKDVTITAFSKMVGYALKAAEELSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEIC SVVE+SFGYLDAPVERI+GADIPMPYAANLERMAVPQ+EDIVR
Sbjct: 301 TVEEGFPQHGVGAEICASVVEDSFGYLDAPVERISGADIPMPYAANLERMAVPQIEDIVR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAVPLAATG
Sbjct: 361 AAKRACYRAVPLAATG 376

BLAST of MC07g0030 vs. ExPASy TrEMBL
Match: A0A6J1H8M4 (Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111461100 PE=4 SV=1)

HSP 1 Score: 704 bits (1817), Expect = 2.71e-255
Identity = 363/376 (96.54%), Postives = 369/376 (98.14%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           MWGI+RQK GA  SSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD
Sbjct: 1   MWGIVRQKAGAAASSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVFLMGEEVGEYQGAYKITKGLL+KYGPERVLDTPITEAGF GIGVGAAYHGLKPVVEF
Sbjct: 61  PKVFLMGEEVGEYQGAYKITKGLLEKYGPERVLDTPITEAGFAGIGVGAAYHGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP
Sbjct: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI
Sbjct: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTITAFSKMVGYALKAAE LSKEGISAEVINLRSIRPLDRATINASVRKT+RLV
Sbjct: 241 EREGKDVTITAFSKMVGYALKAAEELSKEGISAEVINLRSIRPLDRATINASVRKTNRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGVGAEIC SVVE+SFGYLDAPVERI+GADIPMPYAANLERMAVPQ+EDIVR
Sbjct: 301 TVEEGFPQHGVGAEICASVVEDSFGYLDAPVERISGADIPMPYAANLERMAVPQIEDIVR 360

Query: 361 AAKRACYRAVPLAATG 376
           AAKRACYRAV LAATG
Sbjct: 361 AAKRACYRAVRLAATG 376

BLAST of MC07g0030 vs. TAIR 10
Match: AT5G50850.1 (Transketolase family protein )

HSP 1 Score: 607.1 bits (1564), Expect = 9.8e-174
Identity = 312/369 (84.55%), Postives = 337/369 (91.33%), Query Frame = 0

Query: 1   MWGIIRQKVGAGGSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSAD 60
           M GI+RQ+   G S       +L+R R A  ++R Y++ AKEMTVRDALNSA+DEEMSAD
Sbjct: 1   MLGILRQRAIDGAS-------TLRRTRFALVSARSYAAGAKEMTVRDALNSAIDEEMSAD 60

Query: 61  PKVFLMGEEVGEYQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEF 120
           PKVF+MGEEVG+YQGAYKITKGLL+KYGPERV DTPITEAGFTGIGVGAAY GLKPVVEF
Sbjct: 61  PKVFVMGEEVGQYQGAYKITKGLLEKYGPERVYDTPITEAGFTGIGVGAAYAGLKPVVEF 120

Query: 121 MTFNFSMQAIDHIINSAAKTNYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCP 180
           MTFNFSMQAIDHIINSAAK+NYMSAGQI+VPIVFRGPNGAAAGVGAQHSQCYAAWY S P
Sbjct: 121 MTFNFSMQAIDHIINSAAKSNYMSAGQINVPIVFRGPNGAAAGVGAQHSQCYAAWYASVP 180

Query: 181 GLKVLAPYSSEDARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKI 240
           GLKVLAPYS+EDARGLLKAAIRDPDPVVFLENELLYGESFP+S E LDSSF  PIGKAKI
Sbjct: 181 GLKVLAPYSAEDARGLLKAAIRDPDPVVFLENELLYGESFPISEEALDSSFCLPIGKAKI 240

Query: 241 EREGKDVTITAFSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLV 300
           EREGKDVTI  FSKMVG+ALKAAE L++EGISAEVINLRSIRPLDRATINASVRKTSRLV
Sbjct: 241 EREGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLV 300

Query: 301 TVEEGFPQHGVGAEICTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVR 360
           TVEEGFPQHGV AEIC SVVEESF YLDAPVERIAGAD+PMPYAANLER+A+PQ+EDIVR
Sbjct: 301 TVEEGFPQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVR 360

Query: 361 AAKRACYRA 370
           A+KRACYR+
Sbjct: 361 ASKRACYRS 362

BLAST of MC07g0030 vs. TAIR 10
Match: AT1G30120.1 (pyruvate dehydrogenase E1 beta )

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-71
Identity = 142/346 (41.04%), Postives = 216/346 (62.43%), Query Frame = 0

Query: 24  QRLRPAASASRYYSSAAK---EMTVRDALNSALDEEMSADPKVFLMGEEVGEYQGAYKIT 83
           Q+L P A A++   ++A    E+ + +AL   L+EEM  DP V +MGE+VG Y G+YK+T
Sbjct: 64  QKLIPNAVATKEADTSASTGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGHYGGSYKVT 123

Query: 84  KGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEFMTFNFSMQAIDHIINSAAKT 143
           KGL DK+G  RVLDTPI E  FTG+G+GAA  GL+PV+E M   F + A + I N+    
Sbjct: 124 KGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQISNNCGML 183

Query: 144 NYMSAGQISVPIVFRGPNGAAAGVGAQHSQCYAAWYGSCPGLKVLAPYSSEDARGLLKAA 203
           +Y S GQ ++P+V RGP G    +GA+HSQ   +++ S PG++++A  +  +A+GL+KAA
Sbjct: 184 HYTSGGQFTIPVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAA 243

Query: 204 IRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKIEREGKDVTITAFSKMVGYAL 263
           IR  +PV+  E+ LLY     +  ++ D  +   + +A++ R G+ +TI  +S+M  + +
Sbjct: 244 IRSENPVILFEHVLLYN----LKEKIPDEDYVCNLEEAEMVRPGEHITILTYSRMRYHVM 303

Query: 264 KAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVGAEICTSVV 323
           +AA+ L  +G   EVI++RS++P D  TI  SV+KT R++ VEE     G+GA +  ++ 
Sbjct: 304 QAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGASLTAAIN 363

Query: 324 EESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVRAAKRAC 367
           E    YLDAPV  ++  D+P PYA  LE   V Q   IV A ++ C
Sbjct: 364 ENFHDYLDAPVMCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLC 405

BLAST of MC07g0030 vs. TAIR 10
Match: AT2G34590.1 (Transketolase family protein )

HSP 1 Score: 266.5 bits (680), Expect = 3.2e-71
Identity = 142/338 (42.01%), Postives = 209/338 (61.83%), Query Frame = 0

Query: 31  SASRYYSSAAKEMTVRDALNSALDEEMSADPKVFLMGEEVGEYQGAYKITKGLLDKYGPE 90
           +AS   S    E+ + +AL   L+EEM  DP V +MGE+VG Y G+YK+TKGL DK+G  
Sbjct: 74  AASSTSSKPGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGHYGGSYKVTKGLADKFGDL 133

Query: 91  RVLDTPITEAGFTGIGVGAAYHGLKPVVEFMTFNFSMQAIDHIINSAAKTNYMSAGQISV 150
           RVLDTPI E  FTG+G+GAA  GL+PV+E M   F + A + I N+    +Y S GQ ++
Sbjct: 134 RVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQISNNCGMLHYTSGGQFTI 193

Query: 151 PIVFRGPNGAAAGVGAQHSQCYAAWYGSCPGLKVLAPYSSEDARGLLKAAIRDPDPVVFL 210
           P+V RGP G    +GA+HSQ   +++ S PG++++A  +  +A+GL+KAAIR  +PV+  
Sbjct: 194 PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPYNAKGLMKAAIRSENPVILF 253

Query: 211 ENELLYG--ESFPVSAEVLDSSFTAPIGKAKIEREGKDVTITAFSKMVGYALKAAEILSK 270
           E+ LLY   ES P      D  +   + +A++ R G+ +TI  +S+M  + ++AA+ L  
Sbjct: 254 EHVLLYNLKESIP------DEEYICNLEEAEMVRPGEHITILTYSRMRYHVMQAAKTLVN 313

Query: 271 EGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVGAEICTSVVEESFGYLD 330
           +G   EVI++RS++P D  TI  SV+KT R++ VEE     G+GA +  ++ E    YLD
Sbjct: 314 KGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIGASLTAAINENFHDYLD 373

Query: 331 APVERIAGADIPMPYAANLERMAVPQVEDIVRAAKRAC 367
           APV  ++  D+P PYA  LE   V Q   IV A ++ C
Sbjct: 374 APVMCLSSQDVPTPYAGTLEEWTVVQPAQIVTAVEQLC 405

BLAST of MC07g0030 vs. TAIR 10
Match: AT1G55510.1 (branched-chain alpha-keto acid decarboxylase E1 beta subunit )

HSP 1 Score: 211.1 bits (536), Expect = 1.6e-54
Identity = 126/348 (36.21%), Postives = 193/348 (55.46%), Query Frame = 0

Query: 20  GQSLQRLR-PAAS--ASRYYSSAAKEMTVRDALNSALDEEMSADPKVFLMGEEVGEYQGA 79
           G+S ++L  P+ S  A R  +   K + +  A+N AL   +  DP+ ++ GE+VG + G 
Sbjct: 6   GRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVG-FGGV 65

Query: 80  YKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEFMTFNFSMQAIDHIINS 139
           ++ T GL +++G  RV +TP+ E G  G G+G A  G + +VE    ++   A D I+N 
Sbjct: 66  FRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVNE 125

Query: 140 AAKTNYMSAGQISV-PIVFRGPNGAAAGVGAQHSQCYAAWYGSCPGLKVLAPYSSEDARG 199
           AAK  Y S  Q +   +  R P GA    G  HSQ   A++   PG+KV+ P S  +A+G
Sbjct: 126 AAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAKG 185

Query: 200 LLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKIEREGKDVTITAFSKM 259
           LL + IRDP+PVVF E + LY ++     EV +  +  P+ +A++ REG D+T+  +   
Sbjct: 186 LLLSCIRDPNPVVFFEPKWLYRQAV---EEVPEHDYMIPLSEAEVIREGNDITLVGWGAQ 245

Query: 260 VGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGVGAEI 319
           +    +A     KEGIS E+I+L+++ P D+ T+ ASV+KT RL+   E     G GAEI
Sbjct: 246 LTVMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEI 305

Query: 320 CTSVVEESFGYLDAPVERIAGADIPMPYAANLERMAVPQVEDIVRAAK 364
             +++E  F  L+APV R+ G D P P     E   +P    I+ A K
Sbjct: 306 SATILERCFLKLEAPVSRVCGLDTPFPLV--FEPFYMPTKNKILDAIK 347

BLAST of MC07g0030 vs. TAIR 10
Match: AT3G13450.1 (Transketolase family protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.6e-54
Identity = 120/331 (36.25%), Postives = 182/331 (54.98%), Query Frame = 0

Query: 13  GSSILISGQSLQRLRPAASASRYYSSAAKEMTVRDALNSALDEEMSADPKVFLMGEEVGE 72
           GSS  +SG   + L    S     S + K M +  A+N AL   +  DP+ ++ GE+VG 
Sbjct: 12  GSSFPVSGHGYRML----STVENVSESGKSMNLYSAINQALHIALETDPRSYVFGEDVG- 71

Query: 73  YQGAYKITKGLLDKYGPERVLDTPITEAGFTGIGVGAAYHGLKPVVEFMTFNFSMQAIDH 132
           + G ++ T GL +++G  RV +TP+ E G  G G+G A  G + + E    ++   A D 
Sbjct: 72  FGGVFRCTTGLAERFGKSRVFNTPLCEQGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQ 131

Query: 133 IINSAAKTNYMSAGQISV-PIVFRGPNGAAAGVGAQHSQCYAAWYGSCPGLKVLAPYSSE 192
           I+N AAK  Y S  Q +   +  R P GA    G  HSQ   A++   PG+KV+ P S  
Sbjct: 132 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPR 191

Query: 193 DARGLLKAAIRDPDPVVFLENELLYGESFPVSAEVLDSSFTAPIGKAKIEREGKDVTITA 252
           +A+GLL ++IRDP+PVVF E + LY ++     +V +  +  P+ +A++ REG D+T+  
Sbjct: 192 EAKGLLLSSIRDPNPVVFFEPKWLYRQAV---EDVPEDDYMIPLSEAEVMREGSDITLVG 251

Query: 253 FSKMVGYALKAAEILSKEGISAEVINLRSIRPLDRATINASVRKTSRLVTVEEGFPQHGV 312
           +   +    +A      EGIS E+I+L+++ P D+  +  SVRKT RL+   E     G 
Sbjct: 252 WGAQLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGF 311

Query: 313 GAEICTSVVEESFGYLDAPVERIAGADIPMP 343
           GAEI  ++VE  F  L+APV R+ G D P P
Sbjct: 312 GAEIAATIVERCFLRLEAPVSRVCGLDTPFP 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6Z1G74.3e-18287.80Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial OS=Oryza sativ... [more]
Q0J0H41.1e-18086.54Pyruvate dehydrogenase E1 component subunit beta-2, mitochondrial OS=Oryza sativ... [more]
P529045.6e-17489.40Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Pisum sativum... [more]
Q387991.4e-17284.55Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial OS=Arabidopsis... [more]
Q86HX02.2e-12566.67Pyruvate dehydrogenase E1 component subunit beta, mitochondrial OS=Dictyostelium... [more]
Match NameE-valueIdentityDescription
XP_022148931.11.15e-263100.00pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Momordica cha... [more]
XP_023548371.11.43e-25796.81pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita pep... [more]
XP_022953745.12.04e-25796.54pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial [Cucurbita mos... [more]
KAG6576110.15.12e-25796.81Pyruvate dehydrogenase E1 component subunit beta-2, mitochondrial, partial [Cucu... [more]
XP_022991392.11.67e-25696.28pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial isoform X1 [Cu... [more]
Match NameE-valueIdentityDescription
A0A6J1D6U95.56e-264100.00Pyruvate dehydrogenase E1 component subunit beta OS=Momordica charantia OX=3673 ... [more]
A0A6J1GNY39.86e-25896.54Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1JUS08.10e-25796.28Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1KQ711.15e-25696.81Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1H8M42.71e-25596.54Pyruvate dehydrogenase E1 component subunit beta OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT5G50850.19.8e-17484.55Transketolase family protein [more]
AT1G30120.11.1e-7141.04pyruvate dehydrogenase E1 beta [more]
AT2G34590.13.2e-7142.01Transketolase family protein [more]
AT1G55510.11.6e-5436.21branched-chain alpha-keto acid decarboxylase E1 beta subunit [more]
AT3G13450.11.6e-5436.25Transketolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005475Transketolase-like, pyrimidine-binding domainSMARTSM00861Transket_pyr_3coord: 43..218
e-value: 2.1E-63
score: 226.7
IPR005475Transketolase-like, pyrimidine-binding domainPFAMPF02779Transket_pyrcoord: 42..217
e-value: 3.8E-46
score: 157.0
IPR033248Transketolase, C-terminal domainPFAMPF02780Transketolase_Ccoord: 236..358
e-value: 6.0E-43
score: 145.7
NoneNo IPR availableGENE3D3.40.50.970coord: 42..234
e-value: 1.3E-83
score: 280.7
NoneNo IPR availableCDDcd07036TPP_PYR_E1-PDHc-beta_likecoord: 47..213
e-value: 9.4065E-109
score: 314.031
IPR009014Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain IIGENE3D3.40.50.920coord: 235..367
e-value: 4.4E-51
score: 174.4
IPR009014Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain IISUPERFAMILY52922TK C-terminal domain-likecoord: 231..365
IPR027110Pyruvate dehydrogenase E1 component subunit betaPANTHERPTHR11624DEHYDROGENASE RELATEDcoord: 7..371
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 41..222

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC07g0030.1MC07g0030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006086 acetyl-CoA biosynthetic process from pyruvate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005967 mitochondrial pyruvate dehydrogenase complex
molecular_function GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity
molecular_function GO:0003824 catalytic activity