MC06g_new0569 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g_new0569
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	MC06: 27912149 .. 27913759 (-)
RNA-Seq Expression	MC06g_new0569
Synteny	MC06g_new0569

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACTAAATCGATGGGAAGCACTAAGGAAACCTTTGTGTTTTTAGCTTTGATCTCTCTGTTATCTTCTCTTTCGTATTCATTGCCTCTTTCAACTAGAGGGAGATGGATTGTGGATTCGGCCACCGGACGTCGTGTGAAGCTTGTGTGCGTGAATTGGCCTTCCCACACGCAGAGCATGCTTATTGAAGGGCTTGACCGCAGGCCGTTGAAAGACCTTGCTGATGAGGCGGTTAGGTTGAGGTTCAATTGTGTTCGACTCACGTACGCTACTCATATGTTTACTCGCTATGCTAATAGGACGATTGAAGAGAACTTTGACCTTCTTGATCTGAAAGCTTCCAAGGCCGGGTTGGCTTTGCATAATCCGTTTGTTTTGAACATGACCATTTTCGAAGCATATGAAGCTGCAGTTGATGTGCTTGGTGCAAGTGGTTTGATGGTGATTGCTGACAATCATATTAGCCAACCAAGATGGTGCTGCTCTCTTGACGATGGAAATGGTTTCTTTGGAGATCGTTATTTTGACCCAGAAGAATGGTTGGAAGGTCTTCGTTTAGTAGCTCGACACTTTCACAACAAATCAAGTGTAAGTACATATTCAACCCTTTTTACTTTCACAACAAATCAAGAAGTACCTATTGAACCCTTTTTAATTAATTTTGTAGAGGTACCTAGTAACTATAACATACAAATACACACATTTTTATATAAAATATAAGTCATTGTAAATATGATTTTTTACAATGCTAAATAAATATAACCCATCCATAGTCTTCAATAAATTAAATGTTTTATCATCTCGGTAGTTATTCGATATCTCATAATAATGAGATGAACCAAAAATCCAACACATAAATTATTATCATTATGTCAATATGAACATGTAACTCAATAGATAAGACGCATGTTACCTCTTTAAAAGAAGATACAGTCAGAAACATACCACAATTGTATAGAAATAATAATCCATTTGATTTGAACAACACAGGTGGTGGGCATGAGTCTACGAAACGAACTCCGAGGAGCAAGATCAAACCCGAAAGATTGGAACAAGCACATAACACGAGGTGCAAGAGCGATCCACACCATAAACCCAAATGCCCTCGTGATCATCTCGGGCCTAAACTACGACAACGACTTAAGATGCCAAACCCAAGCCCCCTTACCCCTAGGCAACCTCCACAACAAGCTAGTGTTGGAGGTGCACTTATATTCCTTCAGTGGGGACTCCCAAGCAAAGTTCACCCAGAACCCACTCAACAAAATCTGCTCCAAGGTGATGAATGGGTTCGTGGAGAGGGCTGGGTTCGTGGTGGAAGGGGAAAGGGCAGTGCCTTTGCTGGTGAGTGAGTTTGGGATGGACCAGAGGGAGAGTGGGAATGAGGCTGATGACAGGTTTATGAGCTGCTTTGGGGCTTATCTGGCGAGGATGGATTTGGACTGGGCGGTGTGGGGATGGCAAGGGAGTTATTATTATAGGCAGGGGCAGGCTCGGCCTAGGGAAGTGTTTGGGGTCTTGAATTATGATTGGAGTGGTGTCAGAAACCCTAGGTTTTCTCAGAGGTTTCAGCTCTTGCAAACCATGTTGCAAGGTATGTCTTCCTGA

mRNA sequence

ATGGAAACTAAATCGATGGGAAGCACTAAGGAAACCTTTGTGTTTTTAGCTTTGATCTCTCTGTTATCTTCTCTTTCGTATTCATTGCCTCTTTCAACTAGAGGGAGATGGATTGTGGATTCGGCCACCGGACGTCGTGTGAAGCTTGTGTGCGTGAATTGGCCTTCCCACACGCAGAGCATGCTTATTGAAGGGCTTGACCGCAGGCCGTTGAAAGACCTTGCTGATGAGGCGGTTAGGTTGAGGTTCAATTGTGTTCGACTCACGTACGCTACTCATATGTTTACTCGCTATGCTAATAGGACGATTGAAGAGAACTTTGACCTTCTTGATCTGAAAGCTTCCAAGGCCGGGTTGGCTTTGCATAATCCGTTTGTTTTGAACATGACCATTTTCGAAGCATATGAAGCTGCAGTTGATGTGCTTGGTGCAAGTGGTTTGATGGTGATTGCTGACAATCATATTAGCCAACCAAGATGGTGCTGCTCTCTTGACGATGGAAATGGTTTCTTTGGAGATCGTTATTTTGACCCAGAAGAATGGTTGGAAGGTCTTCGTTTAGTAGCTCGACACTTTCACAACAAATCAAGTGTGGTGGGCATGAGTCTACGAAACGAACTCCGAGGAGCAAGATCAAACCCGAAAGATTGGAACAAGCACATAACACGAGGTGCAAGAGCGATCCACACCATAAACCCAAATGCCCTCGTGATCATCTCGGGCCTAAACTACGACAACGACTTAAGATGCCAAACCCAAGCCCCCTTACCCCTAGGCAACCTCCACAACAAGCTAGTGTTGGAGGTGCACTTATATTCCTTCAGTGGGGACTCCCAAGCAAAGTTCACCCAGAACCCACTCAACAAAATCTGCTCCAAGGTGATGAATGGGTTCGTGGAGAGGGCTGGGTTCGTGGTGGAAGGGGAAAGGGCAGTGCCTTTGCTGGTGAGTGAGTTTGGGATGGACCAGAGGGAGAGTGGGAATGAGGCTGATGACAGGTTTATGAGCTGCTTTGGGGCTTATCTGGCGAGGATGGATTTGGACTGGGCGGTGTGGGGATGGCAAGGGAGTTATTATTATAGGCAGGGGCAGGCTCGGCCTAGGGAAGTGTTTGGGGTCTTGAATTATGATTGGAGTGGTGTCAGAAACCCTAGGTTTTCTCAGAGGTTTCAGCTCTTGCAAACCATGTTGCAAGGTATGTCTTCCTGA

Coding sequence (CDS)

ATGGAAACTAAATCGATGGGAAGCACTAAGGAAACCTTTGTGTTTTTAGCTTTGATCTCTCTGTTATCTTCTCTTTCGTATTCATTGCCTCTTTCAACTAGAGGGAGATGGATTGTGGATTCGGCCACCGGACGTCGTGTGAAGCTTGTGTGCGTGAATTGGCCTTCCCACACGCAGAGCATGCTTATTGAAGGGCTTGACCGCAGGCCGTTGAAAGACCTTGCTGATGAGGCGGTTAGGTTGAGGTTCAATTGTGTTCGACTCACGTACGCTACTCATATGTTTACTCGCTATGCTAATAGGACGATTGAAGAGAACTTTGACCTTCTTGATCTGAAAGCTTCCAAGGCCGGGTTGGCTTTGCATAATCCGTTTGTTTTGAACATGACCATTTTCGAAGCATATGAAGCTGCAGTTGATGTGCTTGGTGCAAGTGGTTTGATGGTGATTGCTGACAATCATATTAGCCAACCAAGATGGTGCTGCTCTCTTGACGATGGAAATGGTTTCTTTGGAGATCGTTATTTTGACCCAGAAGAATGGTTGGAAGGTCTTCGTTTAGTAGCTCGACACTTTCACAACAAATCAAGTGTGGTGGGCATGAGTCTACGAAACGAACTCCGAGGAGCAAGATCAAACCCGAAAGATTGGAACAAGCACATAACACGAGGTGCAAGAGCGATCCACACCATAAACCCAAATGCCCTCGTGATCATCTCGGGCCTAAACTACGACAACGACTTAAGATGCCAAACCCAAGCCCCCTTACCCCTAGGCAACCTCCACAACAAGCTAGTGTTGGAGGTGCACTTATATTCCTTCAGTGGGGACTCCCAAGCAAAGTTCACCCAGAACCCACTCAACAAAATCTGCTCCAAGGTGATGAATGGGTTCGTGGAGAGGGCTGGGTTCGTGGTGGAAGGGGAAAGGGCAGTGCCTTTGCTGGTGAGTGAGTTTGGGATGGACCAGAGGGAGAGTGGGAATGAGGCTGATGACAGGTTTATGAGCTGCTTTGGGGCTTATCTGGCGAGGATGGATTTGGACTGGGCGGTGTGGGGATGGCAAGGGAGTTATTATTATAGGCAGGGGCAGGCTCGGCCTAGGGAAGTGTTTGGGGTCTTGAATTATGATTGGAGTGGTGTCAGAAACCCTAGGTTTTCTCAGAGGTTTCAGCTCTTGCAAACCATGTTGCAAGGTATGTCTTCCTGA

Protein sequence

METKSMGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPFVLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS

Homology

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BLAST of MC06g_new0569 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 3.5e-97
Identity = 184/405 (45.43%), Postives = 246/405 (60.74%), Query Frame = 0


Query: 1   METKSMGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQS 60
           M +  +  T    +   L+ L+++ S+SLPL TRGRWIVD ATG RVKL CVNW  H + 
Sbjct: 1   MTSAGVAPTALRLLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEP 60

Query: 61  MLIEGLDRRPLKDLADEAVRLRFNCVRLTYATHMFTR--YANRTIEENFDLLDLKASKAG 120
            L EGL+R P+  +A     L FNCVRLTY+ HM TR  Y N T+ + F  L+L  + +G
Sbjct: 61  GLPEGLNRLPVATVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASG 120

Query: 121 LALHNPFVLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDP 180
           +  +NP +L++    AY   V  L  +G+MVI DNH+S+P+WCC++DDGNGFFGDRYF+P
Sbjct: 121 IEHNNPELLDLGHVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNP 180

Query: 181 EEWLEGLRLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVI 240
             W+EGL L+A +F+N  +VV MSLRNELRG RS P  W++H+  GA  +H  NP  LVI
Sbjct: 181 NTWVEGLGLMATYFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVI 240

Query: 241 ISGLNYDNDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGF 300
           +SGL +D DL      P+ L     K+V E H YSF       +     N +C      F
Sbjct: 241 LSGLQFDTDLSFLPVLPVTL-PFKEKIVYEGHWYSFG----VPWRTGLPNDVCKNETGRF 300

Query: 301 VERAGFVVEGER--AVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQG 360
               GFV       A PL +SEFG+DQR   N+ D+R+++C  AYLA  DLDWA+W   G
Sbjct: 301 KSNVGFVTSSANATAAPLFMSEFGIDQRYV-NDNDNRYLNCILAYLAEEDLDWALWTMGG 360

Query: 361 SYYYRQGQARPR---EVFGVLNYDWSGVRNPRFSQRFQLLQTMLQ 399
           SYYYR  +   +   E +G  N+DWS +RNP F  R + +Q  +Q
Sbjct: 361 SYYYRSDKQPVKDFEETYGFFNHDWSRIRNPDFISRLKEIQQPIQ 399

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BLAST of MC06g_new0569 vs. NCBI nr
Match: XP_022158682.1 (uncharacterized protein LOC111025142 [Momordica charantia])

HSP 1 Score: 810 bits (2092), Expect = 9.22e-294
Identity = 394/397 (99.24%), Postives = 395/397 (99.50%), Query Frame = 0


Query: 6   MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG 65
           MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG
Sbjct: 1   MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG 60

Query: 66  LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF 125
           LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF
Sbjct: 61  LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF 120

Query: 126 VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL 185
           VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL
Sbjct: 121 VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL 180

Query: 186 RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD 245
           RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD
Sbjct: 181 RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD 240

Query: 246 NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV 305
           NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV
Sbjct: 241 NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV 300

Query: 306 VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA 365
           VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA
Sbjct: 301 VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA 360

Query: 366 RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQ  +S
Sbjct: 361 RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQDPNS 397

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BLAST of MC06g_new0569 vs. NCBI nr
Match: XP_004143723.1 (glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical protein Csa_000506 [Cucumis sativus])

HSP 1 Score: 667 bits (1722), Expect = 1.84e-237
Identity = 323/394 (81.98%), Postives = 345/394 (87.56%), Query Frame = 0


Query: 9   TKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 68
           T+  FV LA IS  SSLSYSLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR
Sbjct: 5   TQFGFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 64

Query: 69  RPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPFVLN 128
           RPLKDLA+E VRLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+A+K GLA HNPFVLN
Sbjct: 65  RPLKDLANEVVRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRAAKVGLAFHNPFVLN 124

Query: 129 MTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLV 188
           MTIFEAYEA VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFD EEWLEGLRLV
Sbjct: 125 MTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDTEEWLEGLRLV 184

Query: 189 ARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDL 248
           AR F+NKS+VV MSLRNELRGA S  KDWNK+IT+GA  IH INP  LVIISGLN+DNDL
Sbjct: 185 ARRFYNKSAVVAMSLRNELRGASSKSKDWNKYITQGATTIHNINPKILVIISGLNFDNDL 244

Query: 249 RCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEG 308
           RCQ Q PL L NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSKV+NGFVERA FV+EG
Sbjct: 245 RCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKVINGFVERAEFVMEG 304

Query: 309 ERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPR 368
             AVPL VSEFG+DQR   NEADDRF+SCF A+L   DLDWA+WGWQGSYYYRQG+  P 
Sbjct: 305 AEAVPLFVSEFGLDQR-GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVGPE 364

Query: 369 EVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           EVFGVLNY+WS VRNP FSQ FQLLQTMLQ  +S
Sbjct: 365 EVFGVLNYNWSDVRNPHFSQMFQLLQTMLQDPNS 397

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BLAST of MC06g_new0569 vs. NCBI nr
Match: KAA0055112.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 667 bits (1721), Expect = 2.61e-237
Identity = 321/394 (81.47%), Postives = 347/394 (88.07%), Query Frame = 0


Query: 9   TKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 68
           T+ +FV LA IS  SSLSYSLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR
Sbjct: 5   TQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 64

Query: 69  RPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPFVLN 128
           RPLKDLA+E +RLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+ASK GLALHNPFVLN
Sbjct: 65  RPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNPFVLN 124

Query: 129 MTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLV 188
           MTIFEAYEA VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFD EEWLEGLRLV
Sbjct: 125 MTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEGLRLV 184

Query: 189 ARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDL 248
           AR F+NKS+VV MSLRNELRGA S  KDWNK++T+GA  IH INPN LVIISGLN+DNDL
Sbjct: 185 ARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDL 244

Query: 249 RCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEG 308
           RCQ Q PL L NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSK++NGFV+RA FV+EG
Sbjct: 245 RCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEG 304

Query: 309 ERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPR 368
             AVPL VSEFG+DQ    NEADDRF+SCF A+L   DLDWA+WGWQGSYYYRQG+    
Sbjct: 305 AEAVPLFVSEFGLDQT-GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELE 364

Query: 369 EVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           EVFGVLNY+WS VRNPRFSQ FQLLQTMLQ  +S
Sbjct: 365 EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNS 397

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BLAST of MC06g_new0569 vs. NCBI nr
Match: XP_038875504.1 (glycosyl hydrolase 5 family protein-like [Benincasa hispida])

HSP 1 Score: 664 bits (1713), Expect = 4.00e-236
Identity = 320/398 (80.40%), Postives = 351/398 (88.19%), Query Frame = 0


Query: 6   MGSTKE-TFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIE 65
           MG+T +  FVFLA IS  S LSYS PLST GRWIVDSA GRR+KL+CVNWPSHTQSMLIE
Sbjct: 1   MGNTTQINFVFLAFISFFS-LSYSFPLSTNGRWIVDSA-GRRMKLMCVNWPSHTQSMLIE 60

Query: 66  GLDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNP 125
           GLDRRPLKDLADE VRLRFNCVRLTYATHMFTRYANRT++ENFDLLDLKASK GLALHNP
Sbjct: 61  GLDRRPLKDLADEVVRLRFNCVRLTYATHMFTRYANRTVQENFDLLDLKASKMGLALHNP 120

Query: 126 FVLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEG 185
           F+LNMT+F AYEAAVD+LG SGLMVIADNHISQP+WCCSLDDGNGFFGDRYFDPEEWLEG
Sbjct: 121 FILNMTVFNAYEAAVDILGTSGLMVIADNHISQPKWCCSLDDGNGFFGDRYFDPEEWLEG 180

Query: 186 LRLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNY 245
           LRLVAR F+NKS+VV MSLRNELRG +S  KDWNK++T+GA  IHTINPN L+IISGLN+
Sbjct: 181 LRLVARRFYNKSNVVAMSLRNELRGTKSKSKDWNKYMTQGATTIHTINPNILIIISGLNF 240

Query: 246 DNDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGF 305
           DNDLRCQ Q PLPL NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSK++NGFVERA F
Sbjct: 241 DNDLRCQRQNPLPLNNLHNKLVFEVHLYSFSGESQSKFINNPLNKICSKIINGFVERAEF 300

Query: 306 VVEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQ 365
           V+EG  AVPL VSEFG DQR   NEADDRF+SCF A+LA+ DLDWA+W WQGSYYYRQG+
Sbjct: 301 VMEGAEAVPLFVSEFGFDQR-GVNEADDRFLSCFRAHLAKKDLDWALWAWQGSYYYRQGK 360

Query: 366 ARPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
             P EVFGVLNY+W+ VRNP FSQ FQLLQTMLQ  +S
Sbjct: 361 IEPEEVFGVLNYNWTDVRNPHFSQMFQLLQTMLQDPNS 395

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BLAST of MC06g_new0569 vs. NCBI nr
Match: XP_008467306.1 (PREDICTED: major extracellular endoglucanase-like [Cucumis melo])

HSP 1 Score: 662 bits (1708), Expect = 2.47e-235
Identity = 318/394 (80.71%), Postives = 345/394 (87.56%), Query Frame = 0


Query: 9   TKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 68
           T+ +FV LA I   SSLSYSLPLST GRWIVDSATG RVKLVCVNWPSHTQSMLIEGLDR
Sbjct: 5   TQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIEGLDR 64

Query: 69  RPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPFVLN 128
           RPLKDLA+E +RL+FNCVRLTYATHMFTRYANRT+EENFDLLDL+ASK GLALHNPFVLN
Sbjct: 65  RPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNPFVLN 124

Query: 129 MTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLV 188
           MTIFEAYEA VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFD EEWLEGLRLV
Sbjct: 125 MTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEGLRLV 184

Query: 189 ARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDL 248
           AR F+NKS+VV MSLRNELRGA S  KDWNK++T+GA  IH INPN LVIISGLN+DNDL
Sbjct: 185 ARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDL 244

Query: 249 RCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEG 308
           RCQ Q PL L NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSK++NGFV+RA FV+EG
Sbjct: 245 RCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEG 304

Query: 309 ERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPR 368
             AVPL VSEFG+DQ    NEADDRF+SCF A+L   DLDWA+WGWQGSYYYRQG+    
Sbjct: 305 AEAVPLFVSEFGLDQT-GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELE 364

Query: 369 EVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           EVFGVLNY+WS VRNPRFSQ FQLLQTMLQ  +S
Sbjct: 365 EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNS 397

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BLAST of MC06g_new0569 vs. ExPASy TrEMBL
Match: A0A6J1DXU9 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 PE=3 SV=1)

HSP 1 Score: 810 bits (2092), Expect = 4.46e-294
Identity = 394/397 (99.24%), Postives = 395/397 (99.50%), Query Frame = 0


Query: 6   MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG 65
           MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG
Sbjct: 1   MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG 60

Query: 66  LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF 125
           LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF
Sbjct: 61  LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF 120

Query: 126 VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL 185
           VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL
Sbjct: 121 VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL 180

Query: 186 RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD 245
           RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD
Sbjct: 181 RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD 240

Query: 246 NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV 305
           NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV
Sbjct: 241 NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV 300

Query: 306 VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA 365
           VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA
Sbjct: 301 VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA 360

Query: 366 RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQ  +S
Sbjct: 361 RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQDPNS 397

[Close]

BLAST of MC06g_new0569 vs. ExPASy TrEMBL
Match: A0A5A7UH07 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00380 PE=3 SV=1)

HSP 1 Score: 667 bits (1721), Expect = 1.26e-237
Identity = 321/394 (81.47%), Postives = 347/394 (88.07%), Query Frame = 0


Query: 9   TKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 68
           T+ +FV LA IS  SSLSYSLPLST GRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR
Sbjct: 5   TQFSFVVLAFISFFSSLSYSLPLSTNGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 64

Query: 69  RPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPFVLN 128
           RPLKDLA+E +RLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+ASK GLALHNPFVLN
Sbjct: 65  RPLKDLANEVMRLRFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNPFVLN 124

Query: 129 MTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLV 188
           MTIFEAYEA VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFD EEWLEGLRLV
Sbjct: 125 MTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEGLRLV 184

Query: 189 ARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDL 248
           AR F+NKS+VV MSLRNELRGA S  KDWNK++T+GA  IH INPN LVIISGLN+DNDL
Sbjct: 185 ARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDL 244

Query: 249 RCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEG 308
           RCQ Q PL L NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSK++NGFV+RA FV+EG
Sbjct: 245 RCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEG 304

Query: 309 ERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPR 368
             AVPL VSEFG+DQ    NEADDRF+SCF A+L   DLDWA+WGWQGSYYYRQG+    
Sbjct: 305 AEAVPLFVSEFGLDQT-GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELE 364

Query: 369 EVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           EVFGVLNY+WS VRNPRFSQ FQLLQTMLQ  +S
Sbjct: 365 EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNS 397

[Close]

BLAST of MC06g_new0569 vs. ExPASy TrEMBL
Match: A0A1S3CTF8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV=1)

HSP 1 Score: 662 bits (1708), Expect = 1.20e-235
Identity = 318/394 (80.71%), Postives = 345/394 (87.56%), Query Frame = 0


Query: 9   TKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDR 68
           T+ +FV LA I   SSLSYSLPLST GRWIVDSATG RVKLVCVNWPSHTQSMLIEGLDR
Sbjct: 5   TQFSFVVLAFICFFSSLSYSLPLSTNGRWIVDSATGHRVKLVCVNWPSHTQSMLIEGLDR 64

Query: 69  RPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPFVLN 128
           RPLKDLA+E +RL+FNCVRLTYATHMFTRYANRT+EENFDLLDL+ASK GLALHNPFVLN
Sbjct: 65  RPLKDLANEVMRLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLALHNPFVLN 124

Query: 129 MTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLV 188
           MTIFEAYEA VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFD EEWLEGLRLV
Sbjct: 125 MTIFEAYEAVVDVLGTSGLMVIADNHISQPRWCCSLEDGNGFFGDRYFDSEEWLEGLRLV 184

Query: 189 ARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDL 248
           AR F+NKS+VV MSLRNELRGA S  KDWNK++T+GA  IH INPN LVIISGLN+DNDL
Sbjct: 185 ARRFYNKSAVVAMSLRNELRGASSKSKDWNKYVTQGATTIHNINPNILVIISGLNFDNDL 244

Query: 249 RCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEG 308
           RCQ Q PL L NLHNKLV EVHLYSFSG+SQ+KF  NPLNKICSK++NGFV+RA FV+EG
Sbjct: 245 RCQRQYPLQLNNLHNKLVFEVHLYSFSGESQSKFIHNPLNKICSKIINGFVQRAEFVMEG 304

Query: 309 ERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPR 368
             AVPL VSEFG+DQ    NEADDRF+SCF A+L   DLDWA+WGWQGSYYYRQG+    
Sbjct: 305 AEAVPLFVSEFGLDQT-GVNEADDRFLSCFSAHLVEKDLDWALWGWQGSYYYRQGKVELE 364

Query: 369 EVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           EVFGVLNY+WS VRNPRFSQ FQLLQTMLQ  +S
Sbjct: 365 EVFGVLNYNWSDVRNPRFSQMFQLLQTMLQDPNS 397

[Close]

BLAST of MC06g_new0569 vs. ExPASy TrEMBL
Match: A0A6J1K1F7 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=3 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 4.98e-232
Identity = 315/397 (79.35%), Postives = 346/397 (87.15%), Query Frame = 0


Query: 6   MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG 65
           MGS  +TF+ LA ISL SSLS SLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG
Sbjct: 1   MGSPTQTFMLLAFISLFSSLSNSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG 60

Query: 66  LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF 125
           L  RPLKDLADE V L+FNCVRLTYATHMFTRYANRT+EENFDLLDL+ASKAGLA HNPF
Sbjct: 61  LAHRPLKDLADELVNLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKAGLASHNPF 120

Query: 126 VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL 185
           +LNMTIF+AYEA VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFDP+EWLEGL
Sbjct: 121 ILNMTIFDAYEAVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEGL 180

Query: 186 RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD 245
           RLVAR F NK +VV MSLRNELRGA+S+ KDWNK++T+GA  IH INPN LVI+SGLN+D
Sbjct: 181 RLVARRFTNKLNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFD 240

Query: 246 NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV 305
           NDLRCQ   PLPL NLHNKLV EVHLYSFSG ++ KF  NPLNKICS ++NGFVERA FV
Sbjct: 241 NDLRCQRHNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSAIINGFVERAEFV 300

Query: 306 VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA 365
           ++G  AVPL VSEFG DQR + N ADDRF SCF A+LAR DLDWA+W WQGSYYYRQGQA
Sbjct: 301 MQGAEAVPLFVSEFGFDQRGT-NVADDRFSSCFVAHLARTDLDWALWAWQGSYYYRQGQA 360

Query: 366 RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           +  EVFGVLNY+WS VRNP FS+ FQLLQTML+  +S
Sbjct: 361 QSDEVFGVLNYNWSDVRNPHFSKTFQLLQTMLRDPNS 396

[Close]

BLAST of MC06g_new0569 vs. ExPASy TrEMBL
Match: A0A6J1H201 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 PE=3 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 4.98e-232
Identity = 314/397 (79.09%), Postives = 346/397 (87.15%), Query Frame = 0


Query: 6   MGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEG 65
           MGS  +T + LA ISL SSLS SLPLSTRGRWIVDS TGRRVKLVCVNWPSHTQSMLIEG
Sbjct: 1   MGSPSQTLMLLAFISLFSSLSNSLPLSTRGRWIVDSTTGRRVKLVCVNWPSHTQSMLIEG 60

Query: 66  LDRRPLKDLADEAVRLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKASKAGLALHNPF 125
           L  RPLKDLADE V L+FNCVRLTYATHMFTRYANRT+EENFDLLDL+ASK GLA HNPF
Sbjct: 61  LANRPLKDLADELVSLKFNCVRLTYATHMFTRYANRTVEENFDLLDLRASKVGLASHNPF 120

Query: 126 VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL 185
           VLNMTIFEAYE  VDVLG SGLMVIADNHISQPRWCCSL+DGNGFFGDRYFDP+EWLEGL
Sbjct: 121 VLNMTIFEAYETVVDVLGTSGLMVIADNHISQPRWCCSLNDGNGFFGDRYFDPQEWLEGL 180

Query: 186 RLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYD 245
           RLVAR F NKS+VV MSLRNELRGA+S+ KDWNK++T+GA  IH INPN LVI+SGLN+D
Sbjct: 181 RLVARRFTNKSNVVAMSLRNELRGAKSSSKDWNKYMTQGATTIHDINPNLLVIVSGLNFD 240

Query: 246 NDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFV 305
           NDLRCQ   PLPL NLHNKLV EVHLYSFSG ++ KF  NPLNKICS ++NGFVERA FV
Sbjct: 241 NDLRCQRHNPLPLNNLHNKLVFEVHLYSFSGATKTKFITNPLNKICSTIINGFVERAEFV 300

Query: 306 VEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQA 365
           ++G  AVPL VSEFG DQR + N ADDRF+SCF A+LA+ DLDWA+W WQGSYYYRQGQA
Sbjct: 301 MQGAEAVPLFVSEFGFDQRGT-NVADDRFLSCFVAHLAKTDLDWALWAWQGSYYYRQGQA 360

Query: 366 RPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGMSS 402
           +  EVFG+LNY+WSGVRNPRFS+ FQLLQTML+  +S
Sbjct: 361 QFDEVFGILNYNWSGVRNPRFSKTFQLLQTMLRDPNS 396

[Close]

BLAST of MC06g_new0569 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 337.8 bits (865), Expect = 1.2e-92
Identity = 170/388 (43.81%), Postives = 240/388 (61.86%), Query Frame = 0


Query: 15  FLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDL 74
           F + I+  +  + S PLST  RWIVD   G RVKLVC NWPSH Q ++ EGL ++P+  +
Sbjct: 19  FFSFIAQNTVPNMSYPLSTSSRWIVDE-NGLRVKLVCANWPSHLQPVVAEGLSKQPVDAV 78

Query: 75  ADEAVRLRFNCVRLTYATHMFTRYA---NRTIEENFDLLDLKASKAGLALHNPFVLNMTI 134
           A + V + FNCVRLT+   + T      N T+ ++F  L L     G   +NP ++++ +
Sbjct: 79  AKKIVEMGFNCVRLTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPL 138

Query: 135 FEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARH 194
            EAY+  V  LG + +MVI DNH+++P WCC+ DDGNGFFGD++FDP  W+  L+ +A  
Sbjct: 139 IEAYKTVVTTLGNNDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAAT 198

Query: 195 FHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDLRCQ 254
           F+  S+VVGMSLRNELRG + N  DW K++ +GA A+H+ N   LVI+SGL++D DL   
Sbjct: 199 FNGVSNVVGMSLRNELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFV 258

Query: 255 TQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEGERA 314
              P+ L +   KLV E+H YSFS D  +    NP N IC +V+N      G+++   + 
Sbjct: 259 RSRPVKL-SFTGKLVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLL--NQG 318

Query: 315 VPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPREVF 374
            PL +SEFG+D+R   N  D+R+  C   + A  D+DW++W   GSYY RQG+    E +
Sbjct: 319 FPLFLSEFGIDER-GVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYY 378

Query: 375 GVLNYDWSGVRNPRFSQRFQLLQTMLQG 400
           GVL+ DW  VRN  F Q+   LQ+ LQG
Sbjct: 379 GVLDSDWISVRNSSFLQKISFLQSPLQG 399

[Close]

BLAST of MC06g_new0569 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.9e-92
Identity = 172/396 (43.43%), Postives = 248/396 (62.63%), Query Frame = 0


Query: 11  ETFVFLALISLLSSL-SYSLPLSTRGRWIVDSAT-GRRVKLVCVNWPSHTQSMLIEGLDR 70
           E F F+++  L   + +++ P ST  RWIVD    GRRVKL CVNWPSH ++ + EGL +
Sbjct: 2   EKFFFISVFLLPYVITTFAFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLSK 61

Query: 71  RPLKDLADEAVRLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKASKAGLALHNPF 130
           +PL  +A++ V + FNCVRLT+  ++ T     A  T+ ++     L  + +G   HNP 
Sbjct: 62  QPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNPT 121

Query: 131 VLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGL 190
           +L++ + +A++  V  L    +MVI DNHISQP WCCS +DGNGFFGD++ +P+ W++GL
Sbjct: 122 ILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKGL 181

Query: 191 RLVARHFHNKSS-VVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNY 250
           + +A  F N SS VVGMSLRNELRG + N KDW K++  GA A+H++NPN LVI+SGLNY
Sbjct: 182 KKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLNY 241

Query: 251 DNDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGF 310
             DL    + P  + +   K+V E+H Y F    +     + LNKIC K     ++ +GF
Sbjct: 242 ATDLSFLRERPFEV-SFRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSGF 301

Query: 311 VVEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQ 370
           ++  E+ +PL VSEFG+DQR   N  D++F+SCF A  A  DLDW++W   GSYY R+  
Sbjct: 302 LL--EKGIPLFVSEFGIDQR-GNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKS 361

Query: 371 ARPREVFGVLNYDWSGVRNPRFSQRFQLLQTMLQGM 401
               E +GVL+++WS +RN    Q    +QT   G+
Sbjct: 362 IGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIGL 389

[Close]

BLAST of MC06g_new0569 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.0e-88
Identity = 161/376 (42.82%), Postives = 230/376 (61.17%), Query Frame = 0


Query: 30  PLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKDLADEAVRLRFNCVRLT 89
           PLST  RWI+D   G+RVKL CVNWPSH Q ++ EGL ++ + DLA + + + FNCVR T
Sbjct: 4   PLSTNSRWIIDE-KGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 90  YATHMFTRYA---NRTIEENFDLLDLKASKAGLALHNPFVLNMTIFEAYEAAVDVLGASG 149
           +   + T      N T+ ++F  L L    +G    NP ++++ + EAY+  V  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 150 LMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLVARHFHNKSSVVGMSLRNE 209
           +MVI DNH+++P WCC  +DGNGFFGD +FDP  W+ GL  +A  F   ++VVGMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 210 LRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDLRCQTQAPLPLGNLHNKLV 269
           LRG + N  DW K++ +GA A+H  NPN LVI+SGL+YD DL       + L     KLV
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 270 LEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEGERAVPLLVSEFGMDQRES 329
            E+H YSF+ ++    ++NP N+ C +++       GF +   R  P+ +SEFG+D R  
Sbjct: 244 FELHRYSFT-NTNTWSSKNP-NEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLR-G 303

Query: 330 GNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQGQARPREVFGVLNYDWSGVRNPRF 389
            N  D+R++ C   + A  D+DW++W  QGSYY R+G     E +G+L+ DW  VR+  F
Sbjct: 304 KNVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSF 363

Query: 390 SQRFQLLQTMLQGMSS 403
            QR  L+ + LQG  S
Sbjct: 364 LQRLSLILSPLQGPGS 371

[Close]

BLAST of MC06g_new0569 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 2.0e-87
Identity = 165/394 (41.88%), Postives = 242/394 (61.42%), Query Frame = 0


Query: 4   KSMGSTKETFVFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLI 63
           K++  T   F+FL+LISL  +  Y  PL T+ RWIV++  G RVKL C NWPSH + ++ 
Sbjct: 3   KAIALTVFLFLFLSLISLTLATDY--PLFTKSRWIVNN-KGHRVKLACANWPSHLKPVVA 62

Query: 64  EGLDRRPLKDLADEAVRLRFNCVRLTYATHMF---TRYANRTIEENFDLLDLKASKAGLA 123
           EGL  +P+  ++ +   + FNCVRLT+   +    T   N T++++F+   L     G+ 
Sbjct: 63  EGLSSQPMDSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIY 122

Query: 124 LHNPFVLNMTIFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEE 183
            HNP+++N  +   ++A V  LG   +MVI DNH + P WCCS DD + FFGD  F+P+ 
Sbjct: 123 THNPYIVNTPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDL 182

Query: 184 WLEGLRLVARHFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIIS 243
           W+ GL+ +A  F N  +VVGMSLRNELRG     KDW K++ +GA A+HT NPN LVI+S
Sbjct: 183 WMLGLKKMATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILS 242

Query: 244 GLNYDNDLRCQTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVE 303
           GLN+D DL      P+ L +   KLVLE+H YSF+ D   ++  + +N  CS++ +    
Sbjct: 243 GLNFDADLSFLKDRPVNL-SFKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERR 302

Query: 304 RAGFVVEGERAVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYY 363
             GFV+  ++  PL +SEFG DQR  G+   +R+M+C  A+ A  DLDWAVW   G YY+
Sbjct: 303 TGGFVL--DQGFPLFLSEFGTDQR-GGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYF 362

Query: 364 RQGQARPREVFGVLNYDWSGVRNPRFSQRFQLLQ 395
           R+G+    E +G+L+ +W  V N  + +R  ++Q
Sbjct: 363 REGKRGVVEAYGMLDANWHNVHNYTYLRRLSVIQ 388

[Close]

BLAST of MC06g_new0569 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 3.0e-80
Identity = 151/353 (42.78%), Postives = 218/353 (61.76%), Query Frame = 0


Query: 14  VFLALISLLSSLSYSLPLSTRGRWIVDSATGRRVKLVCVNWPSHTQSMLIEGLDRRPLKD 73
           +F   IS  S L+ S PLST+ RWIVD   G+RVKL CVNWP+H Q  + EGL ++PL  
Sbjct: 9   LFFLFISSTSKLTTSYPLSTKSRWIVDE-KGQRVKLACVNWPAHLQPTVAEGLSKQPLDS 68

Query: 74  LADEAVRLRFNCVRLTYATHMFTR---YANRTIEENFDLLDLKASKAGLALHNPFVLNMT 133
           ++ + V + FNCVRLT+   + T        T++++F+ L L     G+  HNP +L++ 
Sbjct: 69  ISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQTHNPKLLHLP 128

Query: 134 IFEAYEAAVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPEEWLEGLRLVAR 193
           +F A++  V  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W +GLR +A 
Sbjct: 129 LFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVWAKGLRKMAT 188

Query: 194 HFHNKSSVVGMSLRNELRGARSNPKDWNKHITRGARAIHTINPNALVIISGLNYDNDLRC 253
            F N + V+GMSLRNE RGAR  P  W +H+ +GA A+H  NP  LVI+SG+++D +L  
Sbjct: 189 LFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSGIDFDTNLSF 248

Query: 254 QTQAPLPLGNLHNKLVLEVHLYSFSGDSQAKFTQNPLNKICSKVMNGFVERAGFVVEGER 313
                + + +  +KLV E+H YSFS D +  + ++  N  C K++       GF++   R
Sbjct: 249 LRDRSVNV-SFTDKLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHNGGFLL--GR 308

Query: 314 AVPLLVSEFGMDQRESGNEADDRFMSCFGAYLARMDLDWAVWGWQGSYYYRQG 364
             PL++SEFG DQR  G+ + +R+M+C  A+ A  DLDWAVW   G YY R G
Sbjct: 309 GFPLILSEFGTDQR-GGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRTG 355

The following BLAST results are available for this feature:

BLAST of MC06g_new0569 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0HLA0	3.5e-97	45.43	Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]

BLAST of MC06g_new0569 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158682.1	9.22e-294	99.24	uncharacterized protein LOC111025142 [Momordica charantia]	[more]
XP_004143723.1	1.84e-237	81.98	glycosyl hydrolase 5 family protein [Cucumis sativus] >KAE8647936.1 hypothetical...	[more]
KAA0055112.1	2.61e-237	81.47	major extracellular endoglucanase-like [Cucumis melo var. makuwa]	[more]
XP_038875504.1	4.00e-236	80.40	glycosyl hydrolase 5 family protein-like [Benincasa hispida]	[more]
XP_008467306.1	2.47e-235	80.71	PREDICTED: major extracellular endoglucanase-like [Cucumis melo]	[more]

BLAST of MC06g_new0569 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DXU9	4.46e-294	99.24	Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111025142 ...	[more]
A0A5A7UH07	1.26e-237	81.47	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A1S3CTF8	1.20e-235	80.71	Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103504686 PE=3 SV...	[more]
A0A6J1K1F7	4.98e-232	79.35	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111490847 PE=...	[more]
A0A6J1H201	4.98e-232	79.09	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111459707 P...	[more]

BLAST of MC06g_new0569 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13130.1	1.2e-92	43.81	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26130.1	5.9e-92	43.43	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT3G26140.1	3.0e-88	42.82	Cellulase (glycosyl hydrolase family 5) protein	[more]
AT5G17500.1	2.0e-87	41.88	Glycosyl hydrolase superfamily protein	[more]
AT5G16700.1	3.0e-80	42.78	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 70..355 e-value: 1.4E-22 score: 80.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 29..396 e-value: 1.5E-66 score: 227.0
None	No IPR available	PANTHER	PTHR31263:SF0	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 12..399
None	No IPR available	PANTHER	PTHR31263	CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)	coord: 12..399
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 30..385

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g_new0569.1	MC06g_new0569.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016985	mannan endo-1,4-beta-mannosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds