MC06g_new0545 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0545
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionaquaporin PIP1-3-like
LocationMC06: 25918660 .. 25921276 (+)
RNA-Seq ExpressionMC06g_new0545
SyntenyMC06g_new0545
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAAACTATAGCCTAACAAAAAAGAAAAAAAAGTAAAGAAAAGTAACACTCCAAACCCATAATTATTTCACTTTGGGAAAAGAGACCCATTTTGGTCTATAAAAATACCCCATCTGCCAACACGAAAGCCATTGAGTAAAGAACAAAAAAAAGGGAGAGGAGAGAGAAAACTGGTTCTAGAGAGAGAAAGAAGAGTTCAGTGAAGAAGAAGGAAGAAAAAAATGGAGGGTAAGGAAGAGGATGTGAAACTGGGAGCGAACAAGTACTCGGAGAGGCAGCCGATCGGGACGTCGGCGCAGACGGACAAGGACTACAAGGAGCTGCCGCCGGCGCCGCTGTTCGAGCCAGGCGAGCTGACGTCATGGTCGTTTTACAGAGCCGGAATCGCGGAGTTCGTGGCGACATTCTTGTTCCTGTACATCACCATTCTGACGGTGATGGGCGTAAGCCGAGCCCCCACCAAGTGCTCCACCGTCGGCATTCAGGGCATCGCTTGGGCCTTCGGCGGCATGATCTTCGCCCTAGTCTACTGCACCGCCGGCATCTCCGGTAGTTTTTCATTTTCTCGTTCTAATGGAGTTCTCTTAGATCTGTGCGGATGGGAGTAATGTTTTTAGTTAGTATATAACGATTTAGTTGCTATAGTTTAAAATTTGTGACGATAACTTTTAACTGAAAACAAAATGGTTATGAATCGTTATCTTAGTTAGTTTTTACTGTGAAAAATTCTATTAAATTTGAGCATAGTTTTCTAGGTTAAGATTTATATATTTTCCACCAAAACTTGAGAAGTTGGAATTTCCTCCTCCCATATTGAACTAAGAAAGTTCTGTTGAGTATCAAGAATTTATGACAAATTTAAAGAGGAAATGAAATTTCTAGGACTCCGTTTGAAGCAATTTCATTGTGTTTTGTTTGTGTGTGTTTTCAGGTGGGCACATTAACCCGGCGGTGACGTTCGGGCTGTTCTTGGCGAGAAAGCTGTCTCTGACGAGAGCCATTTTCTACATCATTATGCAGTGCCTAGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGCTTCGAGAAGTCGATCTACGAGCAGAAGTTCGGCGGCGCCAACGTCGTCAACCCCGGCTACACCAAGGGCGACGGCCTCGGCGCCGAGATCGTCGGCACCTTCGTTCTCGTCTACACCGTCTTCTCTGCCACTGACGCCAAGAGAAACGCCAGAGATTCTCACGTCCCTGTAAGAAACCAACCCTCAATTTTCTTCTCAAAAAAAGGGGCAAATCAAATAAGATCAAATCAAATAGTAATCTAGGGTTTTTTAATTTCAAAATTGATTTTGAACTTTTTTGGTTGAATATAATGTTGCAGATTTTGGCTCCTCTTCCCATCGGTTTCGCGGTGTTCTTGGTTCATTTGGCCACCATCCCCATCACCGGCACCGGAATTAACCCGGCGCGGAGTCTCGGAGCCGCCATTATCTACAACCGGCAGCACGCTTGGGATGACCATGTGAGTATACAATTGAGCTAAAATTACTACTTTAGCCCCTATAGTATTTGAAACTTGTTTCAATTTTCGTGGTTAAATATTTCAAAATGTCACCCTGTTCTTTTAGTTCAACGTCTGGCGTGGAGTTTCGAGAATCTCTTATATATGCTTTATTAATTTTTTACCCTCTTGTGATGGTCTTAGATAATAGAGTATATAAAAAGTAATTAAGATCTTGAGATCTCCTCTCTAATCTATGTGGAAATTTTCAAATTTGAAAGATCGGATAAAAGAAATGTTAGATTTACGATAAATCGCCAATCAACCCGAAAGCTTAGCTTTAAAGACTTTTGAGTTGTGGTAAATTTCATTTGAATTGGCCAAAATATTAACCATTGTTGGTTAATTAGAGGGTCGAATCCCCTCTACTTTTGTTGATAAACTAAAAAGAGAGTAGATGTTATGCTATGGTTTTATTGATTTTTTTACCCTTTTTGTGATGATCTTAGATTAAAAAGAAGTAAATAAAAAGGAATTAAGATTTTGAGGTCTTCTCTAATCTGTGTGTAAATTTTCGAAAAGAAATATTAGAAGGGAATGTTAAATCACCCATTAATCTTAACATGTAAAACTTTACCTCTGGAAATGGATTATATTCTTGTGGGATGAATGGCTGGTCTGGTTTTGATGAAGGGTTGAGATTTAATGAACAAAATTTTGATGGGTTGTTATATGGTACTACTGTTTGCAGTGGATTTTCTGGGTGGGACCCTTCATTGGGGCTGCTCTTGCTGCTATTTACCACCAGATCATCATCAGAGCCATTCCATTCAAGGCCAGAGCTTGAAGAAGAGCTTCATTTCATCATCATCATCTTCACTCGTCTTATCTTTAGCTCTCTTTTGGTTTTTTTTTTTTTTTTTTGTAATCTTTAATTTCTTGTTTATTTGGTCGTTGAAGAGAGAGATGGATTTGTGTGTAAATTAAGTTATTATCAATGTGCCAGTGGTGTAATTTTGAAGTTTGTAATGGAGAATTATTCTTTTTGTGGATCATCATTGTCTCCAGAACTTTTCATAGTTTCATTTGTGCATGTCACGTGGGATCTCAATCATATATCTTGGCTTTTTGTTATGCAT

mRNA sequence

ATGGAGGGTAAGGAAGAGGATGTGAAACTGGGAGCGAACAAGTACTCGGAGAGGCAGCCGATCGGGACGTCGGCGCAGACGGACAAGGACTACAAGGAGCTGCCGCCGGCGCCGCTGTTCGAGCCAGGCGAGCTGACGTCATGGTCGTTTTACAGAGCCGGAATCGCGGAGTTCGTGGCGACATTCTTGTTCCTGTACATCACCATTCTGACGGTGATGGGCGTAAGCCGAGCCCCCACCAAGTGCTCCACCGTCGGCATTCAGGGCATCGCTTGGGCCTTCGGCGGCATGATCTTCGCCCTAGTCTACTGCACCGCCGGCATCTCCGGTGGGCACATTAACCCGGCGGTGACGTTCGGGCTGTTCTTGGCGAGAAAGCTGTCTCTGACGAGAGCCATTTTCTACATCATTATGCAGTGCCTAGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGCTTCGAGAAGTCGATCTACGAGCAGAAGTTCGGCGGCGCCAACGTCGTCAACCCCGGCTACACCAAGGGCGACGGCCTCGGCGCCGAGATCGTCGGCACCTTCGTTCTCGTCTACACCGTCTTCTCTGCCACTGACGCCAAGAGAAACGCCAGAGATTCTCACGTCCCTATTTTGGCTCCTCTTCCCATCGGTTTCGCGGTGTTCTTGGTTCATTTGGCCACCATCCCCATCACCGGCACCGGAATTAACCCGGCGCGGAGTCTCGGAGCCGCCATTATCTACAACCGGCAGCACGCTTGGGATGACCATTGGATTTTCTGGGTGGGACCCTTCATTGGGGCTGCTCTTGCTGCTATTTACCACCAGATCATCATCAGAGCCATTCCATTCAAGGCCAGAGCTTGA

Coding sequence (CDS)

ATGGAGGGTAAGGAAGAGGATGTGAAACTGGGAGCGAACAAGTACTCGGAGAGGCAGCCGATCGGGACGTCGGCGCAGACGGACAAGGACTACAAGGAGCTGCCGCCGGCGCCGCTGTTCGAGCCAGGCGAGCTGACGTCATGGTCGTTTTACAGAGCCGGAATCGCGGAGTTCGTGGCGACATTCTTGTTCCTGTACATCACCATTCTGACGGTGATGGGCGTAAGCCGAGCCCCCACCAAGTGCTCCACCGTCGGCATTCAGGGCATCGCTTGGGCCTTCGGCGGCATGATCTTCGCCCTAGTCTACTGCACCGCCGGCATCTCCGGTGGGCACATTAACCCGGCGGTGACGTTCGGGCTGTTCTTGGCGAGAAAGCTGTCTCTGACGAGAGCCATTTTCTACATCATTATGCAGTGCCTAGGCGCCATCTGCGGCGCCGGCGTGGTGAAGGGCTTCGAGAAGTCGATCTACGAGCAGAAGTTCGGCGGCGCCAACGTCGTCAACCCCGGCTACACCAAGGGCGACGGCCTCGGCGCCGAGATCGTCGGCACCTTCGTTCTCGTCTACACCGTCTTCTCTGCCACTGACGCCAAGAGAAACGCCAGAGATTCTCACGTCCCTATTTTGGCTCCTCTTCCCATCGGTTTCGCGGTGTTCTTGGTTCATTTGGCCACCATCCCCATCACCGGCACCGGAATTAACCCGGCGCGGAGTCTCGGAGCCGCCATTATCTACAACCGGCAGCACGCTTGGGATGACCATTGGATTTTCTGGGTGGGACCCTTCATTGGGGCTGCTCTTGCTGCTATTTACCACCAGATCATCATCAGAGCCATTCCATTCAAGGCCAGAGCTTGA

Protein sequence

MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVATFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSLGAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Homology
BLAST of MC06g_new0545 vs. ExPASy Swiss-Prot
Match: Q7XSQ9 (Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP1-2 PE=2 SV=3)

HSP 1 Score: 523.1 bits (1346), Expect = 2.0e-147
Identity = 255/288 (88.54%), Postives = 275/288 (95.49%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQ--TDKDYKELPPAPLFEPGELTSWSFYRAGIAEF 60
           MEGKEEDV+LGANK+SERQPIGT+AQ   DKDYKE PPAPLFEPGEL SWSFYRAGIAEF
Sbjct: 1   MEGKEEDVRLGANKFSERQPIGTAAQGSDDKDYKEPPPAPLFEPGELKSWSFYRAGIAEF 60

Query: 61  VATFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVT 120
           +ATFLFLYIT+LTVMGV+ + +KC+TVGIQGIAW+FGGMIFALVYCTAGISGGHINPAVT
Sbjct: 61  MATFLFLYITVLTVMGVNNSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAVT 120

Query: 121 FGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGL 180
           FGLFLARKLSLTRA+FY++MQCLGAICGAGVVKGF+K +YE   GGANVV PGYTKGDGL
Sbjct: 121 FGLFLARKLSLTRALFYMVMQCLGAICGAGVVKGFQKGLYETTGGGANVVAPGYTKGDGL 180

Query: 181 GAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPAR 240
           GAEIVGTF+LVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPAR
Sbjct: 181 GAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPAR 240

Query: 241 SLGAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           SLGAAIIYNR HAWDDHWIFWVGPFIGAALAAIYHQ++IRAIPFK+R+
Sbjct: 241 SLGAAIIYNRGHAWDDHWIFWVGPFIGAALAAIYHQVVIRAIPFKSRS 288

BLAST of MC06g_new0545 vs. ExPASy Swiss-Prot
Match: Q9XF59 (Aquaporin PIP1-2 OS=Zea mays OX=4577 GN=PIP1-2 PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 3.4e-147
Identity = 257/289 (88.93%), Postives = 275/289 (95.16%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQ---TDKDYKELPPAPLFEPGELTSWSFYRAGIAE 60
           MEGKEEDV+LGANK+SERQPIGT+AQ    DKDYKE PPAPLFEPGEL SWSFYRAGIAE
Sbjct: 1   MEGKEEDVRLGANKFSERQPIGTAAQGAADDKDYKEPPPAPLFEPGELKSWSFYRAGIAE 60

Query: 61  FVATFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAV 120
           FVATFLFLYITILTVMGVS++ +KC+TVGIQGIAW+FGGMIFALVYCTAGISGGHINPAV
Sbjct: 61  FVATFLFLYITILTVMGVSKSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAV 120

Query: 121 TFGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDG 180
           TFGLFLARKLSLTRA+FYIIMQCLGA+CGAGVVKGF++ +Y    GGANVV PGYTKGDG
Sbjct: 121 TFGLFLARKLSLTRALFYIIMQCLGAVCGAGVVKGFQQGLYMGNGGGANVVAPGYTKGDG 180

Query: 181 LGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPA 240
           LGAEIVGTF+LVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPA
Sbjct: 181 LGAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPA 240

Query: 241 RSLGAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           RSLGAAIIYNR HAW+DHWIFWVGPFIGAALAAIYHQ+IIRAIPFK+R+
Sbjct: 241 RSLGAAIIYNRDHAWNDHWIFWVGPFIGAALAAIYHQVIIRAIPFKSRS 289

BLAST of MC06g_new0545 vs. ExPASy Swiss-Prot
Match: Q9AQU5 (Aquaporin PIP1-3/PIP1-4 OS=Zea mays OX=4577 GN=PIP1-3 PE=2 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 5.8e-147
Identity = 258/292 (88.36%), Postives = 274/292 (93.84%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQ------TDKDYKELPPAPLFEPGELTSWSFYRAG 60
           MEGKEEDV+LGANK+SERQPIGT+AQ       DKDYKE PPAPLFEPGEL SWSFYRAG
Sbjct: 1   MEGKEEDVRLGANKFSERQPIGTAAQGAGAGDDDKDYKEPPPAPLFEPGELKSWSFYRAG 60

Query: 61  IAEFVATFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHIN 120
           IAEFVATFLFLYIT+LTVMGVS++ +KC+TVGIQGIAW+FGGMIFALVYCTAGISGGHIN
Sbjct: 61  IAEFVATFLFLYITVLTVMGVSKSTSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHIN 120

Query: 121 PAVTFGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTK 180
           PAVTFGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGF++ +Y    GGANVV PGYTK
Sbjct: 121 PAVTFGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFQQGLYMGNGGGANVVAPGYTK 180

Query: 181 GDGLGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGI 240
           GDGLGAEIVGTF+LVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGI
Sbjct: 181 GDGLGAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGI 240

Query: 241 NPARSLGAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           NPARSLGAAIIYNR HAW DHWIFWVGPFIGAALAAIYHQ+IIRAIPFK+R+
Sbjct: 241 NPARSLGAAIIYNRDHAWSDHWIFWVGPFIGAALAAIYHQVIIRAIPFKSRS 292

BLAST of MC06g_new0545 vs. ExPASy Swiss-Prot
Match: Q6EU94 (Aquaporin PIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP1-1 PE=2 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 3.8e-146
Identity = 255/289 (88.24%), Postives = 274/289 (94.81%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQ---TDKDYKELPPAPLFEPGELTSWSFYRAGIAE 60
           MEGKEEDV+LGAN+YSERQPIGT+AQ    DKDYKE PPAPLFEPGEL SWSFYRAGIAE
Sbjct: 1   MEGKEEDVRLGANRYSERQPIGTAAQGAGDDKDYKEPPPAPLFEPGELKSWSFYRAGIAE 60

Query: 61  FVATFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAV 120
           FVATFLFLYITILTVMGVS++ +KC+TVGIQGIAW+FGGMIFALVYCTAGISGGHINPAV
Sbjct: 61  FVATFLFLYITILTVMGVSKSSSKCATVGIQGIAWSFGGMIFALVYCTAGISGGHINPAV 120

Query: 121 TFGLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDG 180
           TFGLFLARKLSLTRAIFYI+MQCLGAICGAGVVKGF++ +Y    GGANVV  GYTKGDG
Sbjct: 121 TFGLFLARKLSLTRAIFYIVMQCLGAICGAGVVKGFQQGLYMGNGGGANVVASGYTKGDG 180

Query: 181 LGAEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPA 240
           LGAEIVGTF+LVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPA
Sbjct: 181 LGAEIVGTFILVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPA 240

Query: 241 RSLGAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           RSLGAAIIYN+ HAW+DHWIFWVGPF+GAALAAIYHQ+IIRAIPFK+R+
Sbjct: 241 RSLGAAIIYNKDHAWNDHWIFWVGPFVGAALAAIYHQVIIRAIPFKSRS 289

BLAST of MC06g_new0545 vs. ExPASy Swiss-Prot
Match: Q08733 (Aquaporin PIP1-3 OS=Arabidopsis thaliana OX=3702 GN=PIP1-3 PE=1 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 1.9e-145
Identity = 249/286 (87.06%), Postives = 270/286 (94.41%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDV++GANK+ ERQPIGTSAQTDKDYKE PPAP FEPGEL+SWSFYRAGIAEF+A
Sbjct: 1   MEGKEEDVRVGANKFPERQPIGTSAQTDKDYKEPPPAPFFEPGELSSWSFYRAGIAEFIA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYIT+LTVMGV RAP  C++VGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITVLTVMGVKRAPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRA+FYI+MQCLGAICGAGVVKGF+ + Y+   GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAVFYIVMQCLGAICGAGVVKGFQPNPYQTLGGGANTVAHGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKR+ARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           GAAIIYN+ HAWDDHWIFWVGPFIGAALAA+YHQ++IRAIPFK+R+
Sbjct: 241 GAAIIYNKDHAWDDHWIFWVGPFIGAALAALYHQLVIRAIPFKSRS 286

BLAST of MC06g_new0545 vs. NCBI nr
Match: XP_022159472.1 (probable aquaporin PIP1-2 [Momordica charantia])

HSP 1 Score: 577 bits (1486), Expect = 1.45e-207
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. NCBI nr
Match: XP_022929031.1 (aquaporin PIP1-3-like [Cucurbita moschata] >XP_022970079.1 aquaporin PIP1-3-like [Cucurbita maxima] >XP_023550107.1 aquaporin PIP1-3-like [Cucurbita pepo subsp. pepo] >KAG6579513.1 Aquaporin PIP1-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 557 bits (1435), Expect = 8.67e-200
Identity = 276/286 (96.50%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKE PPAPL+EPGELTSWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKEPPPAPLYEPGELTSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAP+KCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPSKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKLGGANFVAAGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNR+HAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNREHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. NCBI nr
Match: XP_022957952.1 (aquaporin PIP1-3 [Cucurbita moschata] >XP_022995493.1 aquaporin PIP1-3 [Cucurbita maxima] >XP_023534336.1 aquaporin PIP1-3 [Cucurbita pepo subsp. pepo] >KAG6606086.1 Aquaporin PIP1-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 556 bits (1434), Expect = 1.23e-199
Identity = 277/286 (96.85%), Postives = 279/286 (97.55%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKE PPAPLFEPGEL+SWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKEPPPAPLFEPGELSSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIY QK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYVQKLGGANFVASGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. NCBI nr
Match: XP_008437293.1 (PREDICTED: aquaporin PIP1-2 [Cucumis melo] >KAA0042749.1 aquaporin PIP1-2 [Cucumis melo var. makuwa])

HSP 1 Score: 545 bits (1405), Expect = 3.24e-195
Identity = 270/286 (94.41%), Postives = 277/286 (96.85%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANK+SERQPIGTSAQTDKDYKE PPAPL+EPGELTSWSFYRAGIAEF+A
Sbjct: 1   MEGKEEDVKLGANKFSERQPIGTSAQTDKDYKEPPPAPLYEPGELTSWSFYRAGIAEFMA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSR+P+KC+TVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRSPSKCNTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRA+FYIIMQCLGAICGAGVVKGFE S Y QK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRALFYIIMQCLGAICGAGVVKGFEGSTYVQKLGGANFVASGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. NCBI nr
Match: XP_038875704.1 (aquaporin PIP1-3 [Benincasa hispida])

HSP 1 Score: 544 bits (1401), Expect = 1.32e-194
Identity = 268/285 (94.04%), Postives = 275/285 (96.49%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANK++ERQPIGTSAQTDKDYKE PPAPL+EPGELTSWSFYRAGIAEF+A
Sbjct: 1   MEGKEEDVKLGANKFTERQPIGTSAQTDKDYKEPPPAPLYEPGELTSWSFYRAGIAEFMA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAP+KCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPSKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYI+MQCLGAICGAGVVKGFE S Y QK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAIFYIVMQCLGAICGAGVVKGFEGSTYVQKLGGANFVASGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKAR 285
           GAAIIYNRQHAWDDHWIFWVGPF+GA LAAIYHQIIIRAIPFKAR
Sbjct: 241 GAAIIYNRQHAWDDHWIFWVGPFVGATLAAIYHQIIIRAIPFKAR 285

BLAST of MC06g_new0545 vs. ExPASy TrEMBL
Match: A0A6J1DZX9 (probable aquaporin PIP1-2 OS=Momordica charantia OX=3673 GN=LOC111025877 PE=3 SV=1)

HSP 1 Score: 577 bits (1486), Expect = 7.04e-208
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. ExPASy TrEMBL
Match: A0A6J1I4G7 (aquaporin PIP1-3-like OS=Cucurbita maxima OX=3661 GN=LOC111469079 PE=3 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 4.20e-200
Identity = 276/286 (96.50%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKE PPAPL+EPGELTSWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKEPPPAPLYEPGELTSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAP+KCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPSKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKLGGANFVAAGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNR+HAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNREHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. ExPASy TrEMBL
Match: A0A6J1ELY6 (aquaporin PIP1-3-like OS=Cucurbita moschata OX=3662 GN=LOC111435750 PE=3 SV=1)

HSP 1 Score: 557 bits (1435), Expect = 4.20e-200
Identity = 276/286 (96.50%), Postives = 280/286 (97.90%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKE PPAPL+EPGELTSWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKEPPPAPLYEPGELTSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAP+KCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPSKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKLGGANFVAAGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNR+HAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNREHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. ExPASy TrEMBL
Match: A0A6J1K834 (aquaporin PIP1-3 OS=Cucurbita maxima OX=3661 GN=LOC111491014 PE=3 SV=1)

HSP 1 Score: 556 bits (1434), Expect = 5.96e-200
Identity = 277/286 (96.85%), Postives = 279/286 (97.55%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKE PPAPLFEPGEL+SWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKEPPPAPLFEPGELSSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIY QK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYVQKLGGANFVASGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. ExPASy TrEMBL
Match: A0A6J1H0K5 (aquaporin PIP1-3 OS=Cucurbita moschata OX=3662 GN=LOC111459331 PE=3 SV=1)

HSP 1 Score: 556 bits (1434), Expect = 5.96e-200
Identity = 277/286 (96.85%), Postives = 279/286 (97.55%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKE PPAPLFEPGEL+SWSFYRAGIAEFVA
Sbjct: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKEPPPAPLFEPGELSSWSFYRAGIAEFVA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIY QK GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYVQKLGGANFVASGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286
           GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA
Sbjct: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 286

BLAST of MC06g_new0545 vs. TAIR 10
Match: AT1G01620.1 (plasma membrane intrinsic protein 1C )

HSP 1 Score: 516.5 bits (1329), Expect = 1.3e-146
Identity = 249/286 (87.06%), Postives = 270/286 (94.41%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDV++GANK+ ERQPIGTSAQTDKDYKE PPAP FEPGEL+SWSFYRAGIAEF+A
Sbjct: 1   MEGKEEDVRVGANKFPERQPIGTSAQTDKDYKEPPPAPFFEPGELSSWSFYRAGIAEFIA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYIT+LTVMGV RAP  C++VGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITVLTVMGVKRAPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRA+FYI+MQCLGAICGAGVVKGF+ + Y+   GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRAVFYIVMQCLGAICGAGVVKGFQPNPYQTLGGGANTVAHGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKR+ARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           GAAIIYN+ HAWDDHWIFWVGPFIGAALAA+YHQ++IRAIPFK+R+
Sbjct: 241 GAAIIYNKDHAWDDHWIFWVGPFIGAALAALYHQLVIRAIPFKSRS 286

BLAST of MC06g_new0545 vs. TAIR 10
Match: AT4G00430.1 (plasma membrane intrinsic protein 1;4 )

HSP 1 Score: 511.1 bits (1315), Expect = 5.6e-145
Identity = 249/287 (86.76%), Postives = 271/287 (94.43%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQ-TDKDYKELPPAPLFEPGELTSWSFYRAGIAEFV 60
           MEGKEEDV++GANK+ ERQPIGTSAQ TDKDYKE PPAPLFEPGEL+SWSFYRAGIAEF+
Sbjct: 1   MEGKEEDVRVGANKFPERQPIGTSAQSTDKDYKEPPPAPLFEPGELSSWSFYRAGIAEFI 60

Query: 61  ATFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTF 120
           ATFLFLYIT+LTVMGV RAP  C++VGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTF
Sbjct: 61  ATFLFLYITVLTVMGVKRAPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTF 120

Query: 121 GLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLG 180
           GLFLARKLSLTRA+FY+IMQCLGAICGAGVVKGF+ + Y+   GGAN V  GYTKG GLG
Sbjct: 121 GLFLARKLSLTRAVFYMIMQCLGAICGAGVVKGFQPTPYQTLGGGANTVAHGYTKGSGLG 180

Query: 181 AEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARS 240
           AEI+GTFVLVYTVFSATDAKR+ARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARS
Sbjct: 181 AEIIGTFVLVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARS 240

Query: 241 LGAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           LGAAIIYN+ H+WDDHWIFWVGPFIGAALAA+YHQI+IRAIPFK+++
Sbjct: 241 LGAAIIYNKDHSWDDHWIFWVGPFIGAALAALYHQIVIRAIPFKSKS 287

BLAST of MC06g_new0545 vs. TAIR 10
Match: AT4G23400.1 (plasma membrane intrinsic protein 1;5 )

HSP 1 Score: 510.8 bits (1314), Expect = 7.3e-145
Identity = 248/286 (86.71%), Postives = 268/286 (93.71%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTD-KDYKELPPAPLFEPGELTSWSFYRAGIAEFV 60
           MEGKEEDV +GANK+ ERQPIGT+AQT+ KDYKE PPAP FEPGEL SWSFYRAGIAEF+
Sbjct: 1   MEGKEEDVNVGANKFPERQPIGTAAQTESKDYKEPPPAPFFEPGELKSWSFYRAGIAEFI 60

Query: 61  ATFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTF 120
           ATFLFLY+T+LTVMGV RAP  C++VGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTF
Sbjct: 61  ATFLFLYVTVLTVMGVKRAPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTF 120

Query: 121 GLFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLG 180
           GLFLARKLSLTRA+FYI+MQCLGAICGAGVVKGF+  +Y+   GGANVV  GYTKG GLG
Sbjct: 121 GLFLARKLSLTRALFYIVMQCLGAICGAGVVKGFQPGLYQTNGGGANVVAHGYTKGSGLG 180

Query: 181 AEIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARS 240
           AEIVGTFVLVYTVFSATDAKR+ARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARS
Sbjct: 181 AEIVGTFVLVYTVFSATDAKRSARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARS 240

Query: 241 LGAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKAR 286
           LGAAIIYN+ HAWDDHWIFWVGPFIGAALAA+YHQI+IRAIPFK++
Sbjct: 241 LGAAIIYNKDHAWDDHWIFWVGPFIGAALAALYHQIVIRAIPFKSK 286

BLAST of MC06g_new0545 vs. TAIR 10
Match: AT3G61430.1 (plasma membrane intrinsic protein 1A )

HSP 1 Score: 508.1 bits (1307), Expect = 4.7e-144
Identity = 243/286 (84.97%), Postives = 268/286 (93.71%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDV++GANK+ ERQPIGTSAQ+DKDYKE PPAP FEPGEL+SWSF+RAGIAEF+A
Sbjct: 1   MEGKEEDVRVGANKFPERQPIGTSAQSDKDYKEPPPAPFFEPGELSSWSFWRAGIAEFIA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYIT+LTVMGV R+P  C++VGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITVLTVMGVKRSPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRA++YI+MQCLGAICGAGVVKGF+   Y+   GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRALYYIVMQCLGAICGAGVVKGFQPKQYQALGGGANTVAHGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           GAAIIYN+ H+WDDHW+FWVGPFIGAALAA+YH ++IRAIPFK+R+
Sbjct: 241 GAAIIYNKDHSWDDHWVFWVGPFIGAALAALYHVVVIRAIPFKSRS 286

BLAST of MC06g_new0545 vs. TAIR 10
Match: AT3G61430.2 (plasma membrane intrinsic protein 1A )

HSP 1 Score: 508.1 bits (1307), Expect = 4.7e-144
Identity = 243/286 (84.97%), Postives = 268/286 (93.71%), Query Frame = 0

Query: 1   MEGKEEDVKLGANKYSERQPIGTSAQTDKDYKELPPAPLFEPGELTSWSFYRAGIAEFVA 60
           MEGKEEDV++GANK+ ERQPIGTSAQ+DKDYKE PPAP FEPGEL+SWSF+RAGIAEF+A
Sbjct: 1   MEGKEEDVRVGANKFPERQPIGTSAQSDKDYKEPPPAPFFEPGELSSWSFWRAGIAEFIA 60

Query: 61  TFLFLYITILTVMGVSRAPTKCSTVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120
           TFLFLYIT+LTVMGV R+P  C++VGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG
Sbjct: 61  TFLFLYITVLTVMGVKRSPNMCASVGIQGIAWAFGGMIFALVYCTAGISGGHINPAVTFG 120

Query: 121 LFLARKLSLTRAIFYIIMQCLGAICGAGVVKGFEKSIYEQKFGGANVVNPGYTKGDGLGA 180
           LFLARKLSLTRA++YI+MQCLGAICGAGVVKGF+   Y+   GGAN V  GYTKG GLGA
Sbjct: 121 LFLARKLSLTRALYYIVMQCLGAICGAGVVKGFQPKQYQALGGGANTVAHGYTKGSGLGA 180

Query: 181 EIVGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240
           EI+GTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL
Sbjct: 181 EIIGTFVLVYTVFSATDAKRNARDSHVPILAPLPIGFAVFLVHLATIPITGTGINPARSL 240

Query: 241 GAAIIYNRQHAWDDHWIFWVGPFIGAALAAIYHQIIIRAIPFKARA 287
           GAAIIYN+ H+WDDHW+FWVGPFIGAALAA+YH ++IRAIPFK+R+
Sbjct: 241 GAAIIYNKDHSWDDHWVFWVGPFIGAALAALYHVVVIRAIPFKSRS 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XSQ92.0e-14788.54Probable aquaporin PIP1-2 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP1-2 PE=... [more]
Q9XF593.4e-14788.93Aquaporin PIP1-2 OS=Zea mays OX=4577 GN=PIP1-2 PE=1 SV=1[more]
Q9AQU55.8e-14788.36Aquaporin PIP1-3/PIP1-4 OS=Zea mays OX=4577 GN=PIP1-3 PE=2 SV=1[more]
Q6EU943.8e-14688.24Aquaporin PIP1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP1-1 PE=2 SV=1[more]
Q087331.9e-14587.06Aquaporin PIP1-3 OS=Arabidopsis thaliana OX=3702 GN=PIP1-3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022159472.11.45e-207100.00probable aquaporin PIP1-2 [Momordica charantia][more]
XP_022929031.18.67e-20096.50aquaporin PIP1-3-like [Cucurbita moschata] >XP_022970079.1 aquaporin PIP1-3-like... [more]
XP_022957952.11.23e-19996.85aquaporin PIP1-3 [Cucurbita moschata] >XP_022995493.1 aquaporin PIP1-3 [Cucurbit... [more]
XP_008437293.13.24e-19594.41PREDICTED: aquaporin PIP1-2 [Cucumis melo] >KAA0042749.1 aquaporin PIP1-2 [Cucum... [more]
XP_038875704.11.32e-19494.04aquaporin PIP1-3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DZX97.04e-208100.00probable aquaporin PIP1-2 OS=Momordica charantia OX=3673 GN=LOC111025877 PE=3 SV... [more]
A0A6J1I4G74.20e-20096.50aquaporin PIP1-3-like OS=Cucurbita maxima OX=3661 GN=LOC111469079 PE=3 SV=1[more]
A0A6J1ELY64.20e-20096.50aquaporin PIP1-3-like OS=Cucurbita moschata OX=3662 GN=LOC111435750 PE=3 SV=1[more]
A0A6J1K8345.96e-20096.85aquaporin PIP1-3 OS=Cucurbita maxima OX=3661 GN=LOC111491014 PE=3 SV=1[more]
A0A6J1H0K55.96e-20096.85aquaporin PIP1-3 OS=Cucurbita moschata OX=3662 GN=LOC111459331 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G01620.11.3e-14687.06plasma membrane intrinsic protein 1C [more]
AT4G00430.15.6e-14586.76plasma membrane intrinsic protein 1;4 [more]
AT4G23400.17.3e-14586.71plasma membrane intrinsic protein 1;5 [more]
AT3G61430.14.7e-14484.97plasma membrane intrinsic protein 1A [more]
AT3G61430.24.7e-14484.97plasma membrane intrinsic protein 1A [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 131..150
score: 51.16
coord: 256..276
score: 51.41
coord: 52..71
score: 44.03
coord: 94..118
score: 61.97
coord: 216..238
score: 59.02
coord: 180..198
score: 53.65
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 44..273
e-value: 1.4E-83
score: 280.1
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 56..272
e-value: 1.8E-66
score: 222.1
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 52..276
e-value: 1.13682E-81
score: 244.085
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 37..284
e-value: 1.6E-85
score: 288.3
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 44..278
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 1..285
NoneNo IPR availablePANTHERPTHR45687:SF50AQUAPORIN PIP1-2-RELATEDcoord: 1..285
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 112..120

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0545.1MC06g_new0545.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009414 response to water deprivation
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity