MC06g_new0541 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g_new0541 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 1.2e-83
Identity = 160/365 (43.84%), Postives = 219/365 (60.00%), Query Frame = 0

Query: 10  FSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIR---ENLNTTFSRLPYG 69
           F+ LL L+F LL      + L  C F +IYQLGDS SDTGNLIR   +    T +  PYG
Sbjct: 3   FARLLHLVFSLLVFAGITNGL-ICPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYG 62

Query: 70  ETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTALSSEALL 129
           ETF    TGRCS+G L++D+ A    LPL+NPYL ++    HGVNFAVAG+TAL    L 
Sbjct: 63  ETFPGTPTGRCSDGRLIIDFIATALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLA 122

Query: 130 RNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYNYALFQG 189
              +     +S L  QL W  ++  SIC   ++C +KL++ALF++G IG ND NYA F  
Sbjct: 123 ARGVQVSDIHSHLSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPN 182

Query: 190 KTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDE 249
           +TI+E++  VP + + + N   ++I  G +RVIVPG FPIGC+   L           D+
Sbjct: 183 RTIEEIRAYVPFITEAVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDD 242

Query: 250 LHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEASL 309
           L CL  +N+L+ Y N   + A+  L  E P  VI+Y DYYNA  ++ R+   LG +  SL
Sbjct: 243 LGCLSSLNNLSIYFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSL 302

Query: 310 QKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWIIHDIFPK 369
            K CCGIGG YN++  + CG  GVP+CPNP ++I WDG H T+ AY+ +A ++I  I   
Sbjct: 303 LKCCCGIGGPYNYDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKA 362

Query: 370 LHCIF 372
           L C +
Sbjct: 363 LKCSY 365

BLAST of MC06g_new0541 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 3.7e-77
Identity = 158/365 (43.29%), Postives = 203/365 (55.62%), Query Frame = 0

Query: 5   STTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTFSRLP 64
           STT   SLL+ +LF+     S  H    C  ++IYQ GDSISDTGNLIR           
Sbjct: 2   STTKALSLLVFILFV-----SLVHSSDQCPINSIYQFGDSISDTGNLIR----------- 61

Query: 65  YGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTALSSEA 124
                         NG          A  P   P   R+   N  VNF V+GSTAL+S  
Sbjct: 62  --------------NG---------PASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSF 121

Query: 125 LLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYNYALF 184
                +  P TN+ L  QL W   H  S C       D L+ +LF+VGEIGGNDYNY  F
Sbjct: 122 FSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSS--DCLKHSLFMVGEIGGNDYNYGFF 181

Query: 185 QGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAY 244
           QGK ++E++  +P VV  I     +VI  GA  V+VPGNFP+GC PIYLT F   DT  Y
Sbjct: 182 QGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 241

Query: 245 DELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEA 304
           D+  CL  +N  A  HN+Q++ AI  LR+E P   IVYGDYYNA  ++LR      FD++
Sbjct: 242 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKS 301

Query: 305 SLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWIIHDIF 364
              KSCCG GG YN++  +  G  GVP+C NPH+ ISWDG+HLT+KAY+FM+ ++ + I 
Sbjct: 302 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 365 PKLHC 370
            ++ C
Sbjct: 362 SQIKC 320

BLAST of MC06g_new0541 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 280.0 bits (715), Expect = 3.8e-74
Identity = 143/327 (43.73%), Postives = 203/327 (62.08%), Query Frame = 0

Query: 35  FHAIYQLGDSISDTGNLI---RENLNTTFSRLPYGETFFHGATGRCSNGLLMLDYFALTA 94
           F +I   GDSI+DTGNL+     N     +  PYGETFFH  TGR S+G L++D+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 95  RLPLVNPYLN-RDALTNHGVNFAVAGSTALSSEALLRNKIFSPVTNSSLDHQLGWMHSHF 154
             PLV P+   ++A    GVNFAVAG+TAL    L    I S +TN SL  QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 155 NSICLNERDCIDKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNGVEKV 214
            ++C +  DC D + +AL L+GEIGGNDYN+ALFQ K ++EV+++VP V+ TI + + ++
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDEL-HCLKGMNSLASYHNDQIKHAIE 274
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK +N  + Y+N Q++  + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 275 VLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFNLMKICGLPG 334
            LR+  PH  I+Y DYYNALL + +     GF    L  +CCG+GG+YNFN  + CG  G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 335 VPICPNPHEHISWDGIHLTEKAYKFMA 357
           V  C +P +++++DGIH+TE AY+ ++
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLIS 359

BLAST of MC06g_new0541 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 8.5e-74
Identity = 142/331 (42.90%), Postives = 202/331 (61.03%), Query Frame = 0

Query: 35  FHAIYQLGDSISDTGNLIRENLNTTFSR---LPYGETFFHGATGRCSNGLLMLDYFALTA 94
           F +I   GDSI+DTGNL+  +  T   +   LPYGETFFH  TGR SNG L++D+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 95  RLPLVNP-YLNRDALTNHGVNFAVAGSTALSSEALLRNKIFSPVTNSSLDHQLGWMHSHF 154
             PLV P Y +++A    GVNFAV G+TAL    L    I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 155 NSICLNERDCIDKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNGVEKV 214
            ++C++  DC D + ++L L+GEIGGNDYNYA F GK I+E+K++VP V++TI + + ++
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDEL-HCLKGMNSLASYHNDQIKHAIE 274
           I  G    +VPG FP+GC   YL+ +QT++   YD L  CLK +N  + YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 275 VLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFNLMKICGLPG 334
            L++  PH  I+Y DYYN LL + +     GF    L  +CC +GG +NF L +  G   
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNFTLGRKRGTQV 331

Query: 335 VPICPNPHEHISWDGIHLTEKAYKFMAYWII 361
              C +P +++SWDG+H+TE AY+ MA  I+
Sbjct: 332 PECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of MC06g_new0541 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 3.2e-73
Identity = 151/364 (41.48%), Postives = 217/364 (59.62%), Query Frame = 0

Query: 4   SSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLI----RENLNTT 63
           +S  S    L   LF+ + S  T  P     F +I   GDSI+DTGNL+    R  L  T
Sbjct: 2   ASLDSLVIFLFSTLFVTIVSSETPCP----NFKSIISFGDSIADTGNLVGLSDRNQLPVT 61

Query: 64  FSRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYL-NRDALTNHGVNFAVAGST 123
            +  PYGETFFH  TGR  +G +++D+ A    LP V PY  +++   + GVNFAVAG+T
Sbjct: 62  -AFPPYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGAT 121

Query: 124 ALSSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGND 183
           AL S + L+ +   P TN SL  QL        ++C +  DC D + +AL L+GEIGGND
Sbjct: 122 ALKS-SFLKKRGIQPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGND 181

Query: 184 YNYALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQT 243
           YN+  F  K ++EV+++VP V+ +I + + ++I  G    +VPG FPIGC  +YLT ++T
Sbjct: 182 YNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKT 241

Query: 244 NDTAAYD-ELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAF 303
           ++   YD    CLK +N    YH++++K  +  LR+  PH  I+Y DYYN+LL I +   
Sbjct: 242 SNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPA 301

Query: 304 VLGFDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAY 362
             GF E     +CCGIGG YNFN  + CG  GV  C +P +++ WDG+H+TE AYK++A 
Sbjct: 302 KFGFMERPF-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIAD 358

BLAST of MC06g_new0541 vs. NCBI nr
Match: XP_022158415.1 (GDSL esterase/lipase At5g03980-like [Momordica charantia])

HSP 1 Score: 771 bits (1991), Expect = 1.13e-281
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF
Sbjct: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG
Sbjct: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC06g_new0541 vs. NCBI nr
Match: XP_022156428.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 723 bits (1867), Expect = 9.07e-263
Identity = 349/371 (94.07%), Postives = 355/371 (95.69%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           MA S TTS FSLLLPLLFLLLSS STAHPL ACMF AIYQLGDSISDTGNLIREN NTTF
Sbjct: 1   MAISFTTSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S LPYGETFFHGATGRCSNGLLMLDYFAL A LPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           S EALLRNKI SPVTNSSLDHQLGWMHSHF SICLNERDCI+KLRSALFLVGEIGGNDYN
Sbjct: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           T AYDELHCLKG+NSLASYHNDQIK AIEVL+RENPHTVIVYGDYYNALLWILRHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FDEASLQKSCCGIGGNYNFNLMKICGLPGVP+CPNPHEHISWDGIHLT+KAYKFMAYW+I
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC06g_new0541 vs. NCBI nr
Match: XP_022156343.1 (GDSL esterase/lipase At5g03980-like [Momordica charantia])

HSP 1 Score: 696 bits (1797), Expect = 4.23e-252
Identity = 336/370 (90.81%), Postives = 346/370 (93.51%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           M TS+T+S  SLLLPLLFLLLS  STAH L  CMF AIYQLGDSISDTGNLIREN NTTF
Sbjct: 1   MPTSTTSSSLSLLLPLLFLLLSPXSTAHLLRTCMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SRLPYGETFFHGATGRCSNGLLMLDYFAL   LPLVNPYLN++AL N GVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALDXGLPLVNPYLNKBALKNRGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           SSE L RNKIFSPVTNSSLD QL WMHSHF SICLNERDCI+KLRSALFLVGEIGGNDYN
Sbjct: 121 SSETLFRNKIFSPVTNSSLDRQLXWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           T AYDELHCLKG+NSLASYHNDQIK AIEVL+RENPHTVIVYGDYYNALLWILRHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FDEASLQKSCCGIGGNYNFNL+KICGLP VPICPNPHEHISWDGIHLT+KAYKFMAYW+I
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLIKICGLPDVPICPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCI 370
           HDIFPKLHCI
Sbjct: 361 HDIFPKLHCI 370

BLAST of MC06g_new0541 vs. NCBI nr
Match: XP_022150751.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 671 bits (1731), Expect = 1.40e-242
Identity = 316/338 (93.49%), Postives = 325/338 (96.15%), Query Frame = 0

Query: 34  MFHAIYQLGDSISDTGNLIRENLNTTFSRLPYGETFFHGATGRCSNGLLMLDYFALTARL 93
           MF AIYQ GDSISDTGNLIREN NTTFS LPYGETFFHGATGRCSNGLL LDYFAL A L
Sbjct: 1   MFDAIYQXGDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLLXLDYFALDAGL 60

Query: 94  PLVNPYLNRDALTNHGVNFAVAGSTALSSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSI 153
           PLVNPYLNRDALTNHGVNFAVAGSTALS EALLRNKIFSPVTNSSLDHQLGWMHSHFNSI
Sbjct: 61  PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKIFSPVTNSSLDHQLGWMHSHFNSI 120

Query: 154 CLNERDCIDKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNGVEKVISY 213
           CLNERDCI+KLRSALFLVGEIGGNDYNYALFQGKT+QEVKDMVPD+VQTIKN VEKVISY
Sbjct: 121 CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTMQEVKDMVPDIVQTIKNAVEKVISY 180

Query: 214 GATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDELHCLKGMNSLASYHNDQIKHAIEVLRR 273
           GATRVI+PGNFPIGCLPIYLTGFQTNDT AYDELHCLKG+NSLASYHND+IK  IEVLR 
Sbjct: 181 GATRVIIPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYHNDRIKQVIEVLRM 240

Query: 274 ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFNLMKICGLPGVPIC 333
           ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNF+LMKICGLPGVP+C
Sbjct: 241 ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFDLMKICGLPGVPVC 300

Query: 334 PNPHEHISWDGIHLTEKAYKFMAYWIIHDIFPKLHCIF 371
           PNPHEHISWDGIHLTEKAYKFMAYWII+DIFPKLHCIF
Sbjct: 301 PNPHEHISWDGIHLTEKAYKFMAYWIINDIFPKLHCIF 338

BLAST of MC06g_new0541 vs. NCBI nr
Match: XP_022150750.1 (GDSL esterase/lipase At5g03980-like [Momordica charantia])

HSP 1 Score: 632 bits (1629), Expect = 1.06e-225
Identity = 310/360 (86.11%), Postives = 324/360 (90.00%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           MATSSTTS FSLLLPLLFLLLSSPS AHPL ACMF AIYQLGDSISDTGNLIREN NT F
Sbjct: 1   MATSSTTSSFSLLLPLLFLLLSSPSAAHPLRACMFDAIYQLGDSISDTGNLIRENPNTPF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S   YGE+ FHGATGRCSNGLLMLDYFAL A LP VNPYLN+D LT+HGVNFAVAGSTAL
Sbjct: 61  SHPLYGESLFHGATGRCSNGLLMLDYFALAAGLPSVNPYLNKDGLTDHGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           SSEALLRNKI SPVT SSLDHQL WM SHFNSI LNERDC +KL SALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKILSPVTXSSLDHQLDWMFSHFNSIYLNERDCNEKLGSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTI E KDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLT FQTND
Sbjct: 181 YALFQGKTISEAKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTEFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           T AYDELHCLK +NSLASYHNDQIK AI+VLR+ENPHT+IVYGDYYNA LWILR A +LG
Sbjct: 241 TTAYDELHCLKSLNSLASYHNDQIKQAIDVLRKENPHTIIVYGDYYNAFLWILRDASLLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FD ASLQKSCCGIGG+YNFNL+++C LPGVPICPNP E ISWDGIHLTEK YKFMAYW+I
Sbjct: 301 FDGASLQKSCCGIGGDYNFNLVEMCRLPGVPICPNPDERISWDGIHLTEKTYKFMAYWLI 360

BLAST of MC06g_new0541 vs. ExPASy TrEMBL
Match: A0A6J1DVS3 (GDSL esterase/lipase At5g03980-like OS=Momordica charantia OX=3673 GN=LOC111024903 PE=3 SV=1)

HSP 1 Score: 771 bits (1991), Expect = 5.48e-282
Identity = 371/371 (100.00%), Postives = 371/371 (100.00%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF
Sbjct: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG
Sbjct: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC06g_new0541 vs. ExPASy TrEMBL
Match: A0A6J1DQL3 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111023320 PE=3 SV=1)

HSP 1 Score: 723 bits (1867), Expect = 4.39e-263
Identity = 349/371 (94.07%), Postives = 355/371 (95.69%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           MA S TTS FSLLLPLLFLLLSS STAHPL ACMF AIYQLGDSISDTGNLIREN NTTF
Sbjct: 1   MAISFTTSSFSLLLPLLFLLLSSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S LPYGETFFHGATGRCSNGLLMLDYFAL A LPLVNPYLNRDALTNHGVNFAVAGSTAL
Sbjct: 61  SHLPYGETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           S EALLRNKI SPVTNSSLDHQLGWMHSHF SICLNERDCI+KLRSALFLVGEIGGNDYN
Sbjct: 121 SFEALLRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           T AYDELHCLKG+NSLASYHNDQIK AIEVL+RENPHTVIVYGDYYNALLWILRHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FDEASLQKSCCGIGGNYNFNLMKICGLPGVP+CPNPHEHISWDGIHLT+KAYKFMAYW+I
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCIF 371
           HDIFPKLHCIF
Sbjct: 361 HDIFPKLHCIF 371

BLAST of MC06g_new0541 vs. ExPASy TrEMBL
Match: A0A6J1DQD3 (GDSL esterase/lipase At5g03980-like OS=Momordica charantia OX=3673 GN=LOC111023260 PE=3 SV=1)

HSP 1 Score: 697 bits (1799), Expect = 1.02e-252
Identity = 337/370 (91.08%), Postives = 346/370 (93.51%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           M TS+T+S  SLLLPLLFLLLS  STAH L  CMF AIYQLGDSISDTGNLIREN NTTF
Sbjct: 1   MPTSTTSSSLSLLLPLLFLLLSPXSTAHLLRTCMFDAIYQLGDSISDTGNLIRENPNTTF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           SRLPYGETFFHGATGRCSNGLLMLDYFAL   LPLVNPYLN+DAL N GVNFAVAGSTAL
Sbjct: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALDXGLPLVNPYLNKDALKNRGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           SSE L RNKIFSPVTNSSLD QL WMHSHF SICLNERDCI+KLRSALFLVGEIGGNDYN
Sbjct: 121 SSETLFRNKIFSPVTNSSLDRQLXWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTIQEVKDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND
Sbjct: 181 YALFQGKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           T AYDELHCLKG+NSLASYHNDQIK AIEVL+RENPHTVIVYGDYYNALLWILRHAFVLG
Sbjct: 241 TTAYDELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FDEASLQKSCCGIGGNYNFNL+KICGLP VPICPNPHEHISWDGIHLT+KAYKFMAYW+I
Sbjct: 301 FDEASLQKSCCGIGGNYNFNLIKICGLPDVPICPNPHEHISWDGIHLTQKAYKFMAYWLI 360

Query: 361 HDIFPKLHCI 370
           HDIFPKLHCI
Sbjct: 361 HDIFPKLHCI 370

BLAST of MC06g_new0541 vs. ExPASy TrEMBL
Match: A0A6J1DCG0 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111018810 PE=3 SV=1)

HSP 1 Score: 671 bits (1731), Expect = 6.80e-243
Identity = 316/338 (93.49%), Postives = 325/338 (96.15%), Query Frame = 0

Query: 34  MFHAIYQLGDSISDTGNLIRENLNTTFSRLPYGETFFHGATGRCSNGLLMLDYFALTARL 93
           MF AIYQ GDSISDTGNLIREN NTTFS LPYGETFFHGATGRCSNGLL LDYFAL A L
Sbjct: 1   MFDAIYQXGDSISDTGNLIRENPNTTFSHLPYGETFFHGATGRCSNGLLXLDYFALDAGL 60

Query: 94  PLVNPYLNRDALTNHGVNFAVAGSTALSSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSI 153
           PLVNPYLNRDALTNHGVNFAVAGSTALS EALLRNKIFSPVTNSSLDHQLGWMHSHFNSI
Sbjct: 61  PLVNPYLNRDALTNHGVNFAVAGSTALSFEALLRNKIFSPVTNSSLDHQLGWMHSHFNSI 120

Query: 154 CLNERDCIDKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNGVEKVISY 213
           CLNERDCI+KLRSALFLVGEIGGNDYNYALFQGKT+QEVKDMVPD+VQTIKN VEKVISY
Sbjct: 121 CLNERDCIEKLRSALFLVGEIGGNDYNYALFQGKTMQEVKDMVPDIVQTIKNAVEKVISY 180

Query: 214 GATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDELHCLKGMNSLASYHNDQIKHAIEVLRR 273
           GATRVI+PGNFPIGCLPIYLTGFQTNDT AYDELHCLKG+NSLASYHND+IK  IEVLR 
Sbjct: 181 GATRVIIPGNFPIGCLPIYLTGFQTNDTTAYDELHCLKGLNSLASYHNDRIKQVIEVLRM 240

Query: 274 ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFNLMKICGLPGVPIC 333
           ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNF+LMKICGLPGVP+C
Sbjct: 241 ENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFDLMKICGLPGVPVC 300

Query: 334 PNPHEHISWDGIHLTEKAYKFMAYWIIHDIFPKLHCIF 371
           PNPHEHISWDGIHLTEKAYKFMAYWII+DIFPKLHCIF
Sbjct: 301 PNPHEHISWDGIHLTEKAYKFMAYWIINDIFPKLHCIF 338

BLAST of MC06g_new0541 vs. ExPASy TrEMBL
Match: A0A6J1D9D2 (GDSL esterase/lipase At5g03980-like OS=Momordica charantia OX=3673 GN=LOC111018809 PE=3 SV=1)

HSP 1 Score: 632 bits (1629), Expect = 5.11e-226
Identity = 310/360 (86.11%), Postives = 324/360 (90.00%), Query Frame = 0

Query: 1   MATSSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTF 60
           MATSSTTS FSLLLPLLFLLLSSPS AHPL ACMF AIYQLGDSISDTGNLIREN NT F
Sbjct: 1   MATSSTTSSFSLLLPLLFLLLSSPSAAHPLRACMFDAIYQLGDSISDTGNLIRENPNTPF 60

Query: 61  SRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTAL 120
           S   YGE+ FHGATGRCSNGLLMLDYFAL A LP VNPYLN+D LT+HGVNFAVAGSTAL
Sbjct: 61  SHPLYGESLFHGATGRCSNGLLMLDYFALAAGLPSVNPYLNKDGLTDHGVNFAVAGSTAL 120

Query: 121 SSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYN 180
           SSEALLRNKI SPVT SSLDHQL WM SHFNSI LNERDC +KL SALFLVGEIGGNDYN
Sbjct: 121 SSEALLRNKILSPVTXSSLDHQLDWMFSHFNSIYLNERDCNEKLGSALFLVGEIGGNDYN 180

Query: 181 YALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTND 240
           YALFQGKTI E KDMVPDVVQTIKN VEKVISYGATRVIVPGNFPIGCLPIYLT FQTND
Sbjct: 181 YALFQGKTISEAKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTEFQTND 240

Query: 241 TAAYDELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLG 300
           T AYDELHCLK +NSLASYHNDQIK AI+VLR+ENPHT+IVYGDYYNA LWILR A +LG
Sbjct: 241 TTAYDELHCLKSLNSLASYHNDQIKQAIDVLRKENPHTIIVYGDYYNAFLWILRDASLLG 300

Query: 301 FDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWII 360
           FD ASLQKSCCGIGG+YNFNL+++C LPGVPICPNP E ISWDGIHLTEK YKFMAYW+I
Sbjct: 301 FDGASLQKSCCGIGGDYNFNLVEMCRLPGVPICPNPDERISWDGIHLTEKTYKFMAYWLI 360

BLAST of MC06g_new0541 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 2.6e-78
Identity = 158/365 (43.29%), Postives = 203/365 (55.62%), Query Frame = 0

Query: 5   STTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLIRENLNTTFSRLP 64
           STT   SLL+ +LF+     S  H    C  ++IYQ GDSISDTGNLIR           
Sbjct: 2   STTKALSLLVFILFV-----SLVHSSDQCPINSIYQFGDSISDTGNLIR----------- 61

Query: 65  YGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYLNRDALTNHGVNFAVAGSTALSSEA 124
                         NG          A  P   P   R+   N  VNF V+GSTAL+S  
Sbjct: 62  --------------NG---------PASSPTPKPLPQRE--HNVFVNFGVSGSTALNSSF 121

Query: 125 LLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGNDYNYALF 184
                +  P TN+ L  QL W   H  S C       D L+ +LF+VGEIGGNDYNY  F
Sbjct: 122 FSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSS--DCLKHSLFMVGEIGGNDYNYGFF 181

Query: 185 QGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAY 244
           QGK ++E++  +P VV  I     +VI  GA  V+VPGNFP+GC PIYLT F   DT  Y
Sbjct: 182 QGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKDY 241

Query: 245 DELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEA 304
           D+  CL  +N  A  HN+Q++ AI  LR+E P   IVYGDYYNA  ++LR      FD++
Sbjct: 242 DDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSE---RFDKS 301

Query: 305 SLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAYWIIHDIF 364
              KSCCG GG YN++  +  G  GVP+C NPH+ ISWDG+HLT+KAY+FM+ ++ + I 
Sbjct: 302 VALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQIL 320

Query: 365 PKLHC 370
            ++ C
Sbjct: 362 SQIKC 320

BLAST of MC06g_new0541 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.7e-75
Identity = 143/327 (43.73%), Postives = 203/327 (62.08%), Query Frame = 0

Query: 35  FHAIYQLGDSISDTGNLI---RENLNTTFSRLPYGETFFHGATGRCSNGLLMLDYFALTA 94
           F +I   GDSI+DTGNL+     N     +  PYGETFFH  TGR S+G L++D+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 95  RLPLVNPYLN-RDALTNHGVNFAVAGSTALSSEALLRNKIFSPVTNSSLDHQLGWMHSHF 154
             PLV P+   ++A    GVNFAVAG+TAL    L    I S +TN SL  QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 155 NSICLNERDCIDKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNGVEKV 214
            ++C +  DC D + +AL L+GEIGGNDYN+ALFQ K ++EV+++VP V+ TI + + ++
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDEL-HCLKGMNSLASYHNDQIKHAIE 274
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK +N  + Y+N Q++  + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 275 VLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFNLMKICGLPG 334
            LR+  PH  I+Y DYYNALL + +     GF    L  +CCG+GG+YNFN  + CG  G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 335 VPICPNPHEHISWDGIHLTEKAYKFMA 357
           V  C +P +++++DGIH+TE AY+ ++
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLIS 359

BLAST of MC06g_new0541 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 6.1e-75
Identity = 142/331 (42.90%), Postives = 202/331 (61.03%), Query Frame = 0

Query: 35  FHAIYQLGDSISDTGNLIRENLNTTFSR---LPYGETFFHGATGRCSNGLLMLDYFALTA 94
           F +I   GDSI+DTGNL+  +  T   +   LPYGETFFH  TGR SNG L++D+ A   
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 95  RLPLVNP-YLNRDALTNHGVNFAVAGSTALSSEALLRNKIFSPVTNSSLDHQLGWMHSHF 154
             PLV P Y +++A    GVNFAV G+TAL    L    I  P TN SL  QL       
Sbjct: 92  GFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSSFKESL 151

Query: 155 NSICLNERDCIDKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNGVEKV 214
            ++C++  DC D + ++L L+GEIGGNDYNYA F GK I+E+K++VP V++TI + + ++
Sbjct: 152 PNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETISSAITEL 211

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDEL-HCLKGMNSLASYHNDQIKHAIE 274
           I  G    +VPG FP+GC   YL+ +QT++   YD L  CLK +N  + YH++Q++  + 
Sbjct: 212 IGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDPLTGCLKWLNKFSEYHDEQLQAELN 271

Query: 275 VLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFNLMKICGLPG 334
            L++  PH  I+Y DYYN LL + +     GF    L  +CC +GG +NF L +  G   
Sbjct: 272 RLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPL-PACCALGGPFNFTLGRKRGTQV 331

Query: 335 VPICPNPHEHISWDGIHLTEKAYKFMAYWII 361
              C +P +++SWDG+H+TE AY+ MA  I+
Sbjct: 332 PECCDDPSKYVSWDGVHMTEAAYRLMAEGIL 361

BLAST of MC06g_new0541 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 2.3e-74
Identity = 151/364 (41.48%), Postives = 217/364 (59.62%), Query Frame = 0

Query: 4   SSTTSCFSLLLPLLFLLLSSPSTAHPLGACMFHAIYQLGDSISDTGNLI----RENLNTT 63
           +S  S    L   LF+ + S  T  P     F +I   GDSI+DTGNL+    R  L  T
Sbjct: 2   ASLDSLVIFLFSTLFVTIVSSETPCP----NFKSIISFGDSIADTGNLVGLSDRNQLPVT 61

Query: 64  FSRLPYGETFFHGATGRCSNGLLMLDYFALTARLPLVNPYL-NRDALTNHGVNFAVAGST 123
            +  PYGETFFH  TGR  +G +++D+ A    LP V PY  +++   + GVNFAVAG+T
Sbjct: 62  -AFPPYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGAT 121

Query: 124 ALSSEALLRNKIFSPVTNSSLDHQLGWMHSHFNSICLNERDCIDKLRSALFLVGEIGGND 183
           AL S + L+ +   P TN SL  QL        ++C +  DC D + +AL L+GEIGGND
Sbjct: 122 ALKS-SFLKKRGIQPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNALILMGEIGGND 181

Query: 184 YNYALFQGKTIQEVKDMVPDVVQTIKNGVEKVISYGATRVIVPGNFPIGCLPIYLTGFQT 243
           YN+  F  K ++EV+++VP V+ +I + + ++I  G    +VPG FPIGC  +YLT ++T
Sbjct: 182 YNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKT 241

Query: 244 NDTAAYD-ELHCLKGMNSLASYHNDQIKHAIEVLRRENPHTVIVYGDYYNALLWILRHAF 303
           ++   YD    CLK +N    YH++++K  +  LR+  PH  I+Y DYYN+LL I +   
Sbjct: 242 SNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPA 301

Query: 304 VLGFDEASLQKSCCGIGGNYNFNLMKICGLPGVPICPNPHEHISWDGIHLTEKAYKFMAY 362
             GF E     +CCGIGG YNFN  + CG  GV  C +P +++ WDG+H+TE AYK++A 
Sbjct: 302 KFGFMERPF-PACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIAD 358

BLAST of MC06g_new0541 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 1.5e-73
Identity = 142/332 (42.77%), Postives = 196/332 (59.04%), Query Frame = 0

Query: 35  FHAIYQLGDSISDTGNLIRENLNTTFSRL---PYGETFFHGATGRCSNGLLMLDYFALTA 94
           F +I   GDSI+DTGNL+  +       +   PYGE FFH  TGR SNG L++D+ A   
Sbjct: 35  FKSIISFGDSIADTGNLLGLSDPKDLPHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFL 94

Query: 95  RLPLVNP-YLNRDALTNHGVNFAVAGSTALSSEALLRNKIFSPVTNSSLDHQLGWMHSHF 154
            LPLV P Y + +A    GVNFAV G+TAL    L    I  P TN SL  QL       
Sbjct: 95  GLPLVPPFYGSHNANFEKGVNFAVGGATALERSFLEDRGIHFPYTNVSLGVQLNSFKESL 154

Query: 155 NSICLNERDCIDKLRSALFLVGEIGGNDYNYALFQGKTIQEVKDMVPDVVQTIKNGVEKV 214
            SIC +  DC D + +AL L+GEIGGNDYNYA F  K I+E+K+++P V+ TI + + ++
Sbjct: 155 PSICGSPSDCRDMIENALILMGEIGGNDYNYAFFVDKGIEEIKELMPLVITTISSAITEL 214

Query: 215 ISYGATRVIVPGNFPIGCLPIYLTGFQTNDTAAYDEL-HCLKGMNSLASYHNDQIKHAIE 274
           I  G    +VPG FP+GC  +YLT  QT++   YD L  CLK +N     H +Q++  + 
Sbjct: 215 IGMGGRTFLVPGEFPVGCSVLYLTSHQTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELN 274

Query: 275 VLRRENPHTVIVYGDYYNALLWILRHAFVLGFDEASLQKSCCGIGGNYNFNLMKICGLPG 334
            L++  PH  I+Y DYYNAL  + +     GF    L  +CCG GG YN+ + + CG   
Sbjct: 275 RLQKLYPHVNIIYADYYNALFHLYQEPAKFGFMNRPL-SACCGAGGPYNYTVGRKCGTDI 334

Query: 335 VPICPNPHEHISWDGIHLTEKAYKFMAYWIIH 362
           V  C +P ++++WDG+H+TE AY+ MA  I++
Sbjct: 335 VESCDDPSKYVAWDGVHMTEAAYRLMAEGILN 365