MC06g_new0441 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0441
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRNase H domain-containing protein
LocationMC06: 19709275 .. 19712569 (-)
RNA-Seq ExpressionMC06g_new0441
SyntenyMC06g_new0441
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGCAAAACTCAGTGGCGTGCCTCCTTCAACGTGAGGGAAGATGGAATGAAAATATTGTTCGTGAAAACTTTTGTGCTGAGGAAGCTGAAATGATTCTAAAGATCCCTCTGCCCCAGAAAAGTCAAGAGGATGAAATAATATGGAACATGGACAAGAGAGGAATTTTTCGGTCAAAAGTGCATATAACCTACCAACGGTTAATAAAGGTCAATGGTGGAAACTATTTTGGAAGATTCCAGTGCAACTTGAGGTCAAAATAAGTTGTCGGTTACTCGAAGCAGAATCCTAATTACAGCCTGGCAAGTTTGGGAAGCAAGAAATAGAATCATTTTTAAAGGCGAATTTCCAGAGATCATAGAAGTTCAACAGAGATGCAAATCAAGGAATTCGTAGACCTGGGGAAAGGGAGAGAGAGAGATGCATCTGTCAAGCGCTTCACCTCAAGGACGCCCAACTCATAGAAGATTTATACAGATTCAGTGTGGCAAGCAGACAAAGGGTTGGATTATTAGAGATGAGAGGTGGAGATGAAAGAGAAGTTACTTACCTTAGAACTTATGGCAATTTGCAAAGGGATGGAAGCGGCGACAGAAGAAACACCCAAACCCATTCTCTTCCAAATAGATTCTTTGGAAGCATTCCACTTGATTCAAGGCTGATCAACCACATAAGACGTGATGAAAATCGTGTAGTTGGCCAGTGGTTTCATAGTTTCCTGTCCTGGATAACGGGAAATTGACTCAAATAACCCTCCAAAGTTTCTGAAATGCACAAATAACCCACCTCCTTTAATTGAGCCCAAATAACCCACTTTAATATTTTATTGAATTTAAATGAAGTAAAATTACATTAGTACCCCTCAATTCAAAAAAAAAAAAAAAAGAAACTGTGTCACTTTCTTTCTCATCTCCTTCGTTCGTCTGTGTTCCGTTTTCTTCATCTTCTCCGTTCGAGCTTCCCTTTCTTCTCCGTCTGAACTTTCTTTCTCCATTCTTCCATTGCTAATTCGTCTTGCAAGAAAGTTAATTTGGAGATTTGAACTTCGAGGTTCGTTTGATTTCTTAACGTAGTTTAGTTGTTGCTCGATTGCGAAGATCAGGGCTTTATTTTTTATTGCTTCTGAGAAGTTAATTGAAGCTTACCTTTGTCGATTTATTGCGTCTAAGAAACTATCCCGAAGTTAATTTTTTTTCTGAACATAGTTTAGTTGTTGCTCGATTTGGACTTGGATTTCATTTTTTGGAAACGAAATATGCTTTCTGTATTTGCTATTGATCTGTTACTTTGTCTGTGATTCTTCTGTGTTGTGGTGTGGTGGAATGTCTGTTGAAATGTCGGAGGTTGAGGTGATGGGAGGTTGAAGAAGACTGTACCATAGCCGAAGTAGGAGTAACCTTGAGCGTGGTTTAGGGTTTTGGAGGGGTTTTTGCTATCTGTTTATTTTCTCGGGCTCGACCTCGGGCGAGGTTTAGCCCGAGATAATTTGAATAGGCTCAACTCAACCTCGAAGTTGAGCCTCGAGGTTGAGCATGATTCAATTACCTCGGGCTGAACCTCGCTCGAGGTTGAGCTCGAGAAAATAAACAGACAGCAAAAAAAACCCTGGAATAGCTTTCAAATTTTTGTATATTGTACATTTATCTTCATATATTTTAGTGTGTACTTTGTTTGATTTAATTTGAATGTACATGTTTGTTTGTATTTACTTATAGGAAATGGAAATGGTATCGAAGGTCCCTCCCGCGCTGTATGCCTCTTCCCAACTGACCTGTCTATCGCACTTAACGAAGACAGTCGAGGCCATTAAAAAGAAACTTACCCCCCGTCAGATGCGTCTATTTAGGAAGACTGTAGTTGGCCATCTGCTTGATGTGGACCTTGTCTTTAACGGACCACTAATCCATAGTTTGCTGCTTAGGGAGGTGAATGAGAGTCTCCCAGACACCATTAGCTTAAACTTATTTGGGAGTAAGGTGTCCTTTGGTCGGAGAGAATTCGACATAATTAGCGGACTAAAATATTCGAGATCTCCCGTAGGAAGAACACCTTTCCCGTTAGGCTTAGGGCTTTGTACTTTGATAATAAAGTTGACCTGAGCTTGGGTGAATTTGAGAAGAAGTACGAAGTGGCGCGTTTTGAGGATGATTGAGATGCAGTGAAGGTATCAATCGTATACTTAGCTGAATTTGTACTTTACGGTCGGGAGCGAAGCATGAAGTACGACAATAGTTTGCTTGGAACAACAGACGATTGGGAAGTGTGCTGCAACCATGATTGGGGACAGCTATCGTTCGAGAAGACAATAAGAAGTCTGCAGCGAGCACTGACGAAGAAGACAAAGGAGGGGAGGTTGAGGAAATCGTATAGTCTGTACGGTTTCCCGTGGGTATTCCAGGTAAATAAATATATGTTAATCTGCACAGTAATTTGGACCTAGTAATTGCTATTAACATTCTTTGAAATTTATACAGGTGTGGGGGTACGAGATTATATCTTCTATGACTGGACGAGTTGCTAGGAAGATTAGTGACGATGTCATCCCCCGTATGCTCCGGTGGAGGTGCGGTCACTCGACAGGATGGGTCGTGTTGGAGAGGGACATCTTCCAGTCTACAACAGTAAGTGACACACTTGAATTTTATTTGATTTTGAACTGTACCGACTGAAGCGGAGAGGACGTTTTTGAACAGGGTATGCGAGCCACCAACATCAGATGAGGTGGAAATGGATGAAGAAGTCCCCGAACCTTCAAAGACTGCATGTGGGGGTCAGGACAATGTTGGTGCTCCCACTGATGCCGCTATCGATGATACACAGGACGGAGAGGGCACAAAGAAGAAAGATAAAAATAAAAAGAAGAGTAACAAGAAAGTGTTCAGGCGACTTAGAAGTTTGGACATGCGTATGGGCGCTATGGACACGCGTATGAGTGCTATGGAGAAGAGGCTAGAAGGTGTGAAGGGTGAGCTGAAATCCATACGCAAGTATTTGAGACGGATTGCCAAGGTAAGTTTTTATTAATTAAGTTTGTATTATATTTCGTTTTCTCCATCGCCCTCATGCAATATGTCATTTTACGTACAGGGTCAACTAGTCGACCCGGCCGACATGCGAAGAGGTAAGGGAGGTGACGCTGATGGTGGAGCGGGAAGTACGGGTGATGGAGGGTATACTCAAGGGGATGCAACGGACAGTTATCCTAAAGGTGACGGACGTGGGGATGCAGCGGGCGATGGACGTGGGGATGGTGCATCTGATGTTCATGACGACACAATTGTGCAAAATGTCTAG

mRNA sequence

ATGAGGCAAAACTCAGTGGCGTGCCTCCTTCAACGTGAGGGAAGATGGAATGAAAATATTGTTCGTGAAAACTTTTGTGCTGAGGAAGCTGAAATGATTCTAAAGATCCCTCTGCCCCAGAAAAGTCAAGAGGATGAAATAATATGGAACATGGACAAGAGAGGAATTTTTCGGTCAAAAGTGCATATAACCTACCAACGGTTAATAAAGGTCAATGGTGGAAACTATTTTGGAAGATTCCAGTGCAACTTGAGGGTATGCGAGCCACCAACATCAGATGAGGTGGAAATGGATGAAGAAGTCCCCGAACCTTCAAAGACTGCATGTGGGGGTCAGGACAATGTTGGTGCTCCCACTGATGCCGCTATCGATGATACACAGGACGGAGAGGGCACAAAGAAGAAAGATAAAAATAAAAAGAAGAGTAACAAGAAAGTGTTCAGGCGACTTAGAAGTTTGGACATGCGTATGGGCGCTATGGACACGCGTATGAGTGCTATGGAGAAGAGGCTAGAAGGTGTGAAGGGTGAGCTGAAATCCATACGCAAGTATTTGAGACGGATTGCCAAGGGTCAACTAGTCGACCCGGCCGACATGCGAAGAGGTAAGGGAGGTGACGCTGATGGTGGAGCGGGAAGTACGGGTGATGGAGGGTATACTCAAGGGGATGCAACGGACAGTTATCCTAAAGGTGACGGACGTGGGGATGCAGCGGGCGATGGACGTGGGGATGGTGCATCTGATGTTCATGACGACACAATTGTGCAAAATGTCTAG

Coding sequence (CDS)

ATGAGGCAAAACTCAGTGGCGTGCCTCCTTCAACGTGAGGGAAGATGGAATGAAAATATTGTTCGTGAAAACTTTTGTGCTGAGGAAGCTGAAATGATTCTAAAGATCCCTCTGCCCCAGAAAAGTCAAGAGGATGAAATAATATGGAACATGGACAAGAGAGGAATTTTTCGGTCAAAAGTGCATATAACCTACCAACGGTTAATAAAGGTCAATGGTGGAAACTATTTTGGAAGATTCCAGTGCAACTTGAGGGTATGCGAGCCACCAACATCAGATGAGGTGGAAATGGATGAAGAAGTCCCCGAACCTTCAAAGACTGCATGTGGGGGTCAGGACAATGTTGGTGCTCCCACTGATGCCGCTATCGATGATACACAGGACGGAGAGGGCACAAAGAAGAAAGATAAAAATAAAAAGAAGAGTAACAAGAAAGTGTTCAGGCGACTTAGAAGTTTGGACATGCGTATGGGCGCTATGGACACGCGTATGAGTGCTATGGAGAAGAGGCTAGAAGGTGTGAAGGGTGAGCTGAAATCCATACGCAAGTATTTGAGACGGATTGCCAAGGGTCAACTAGTCGACCCGGCCGACATGCGAAGAGGTAAGGGAGGTGACGCTGATGGTGGAGCGGGAAGTACGGGTGATGGAGGGTATACTCAAGGGGATGCAACGGACAGTTATCCTAAAGGTGACGGACGTGGGGATGCAGCGGGCGATGGACGTGGGGATGGTGCATCTGATGTTCATGACGACACAATTGTGCAAAATGTCTAG

Protein sequence

MRQNSVACLLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIFRSKVHITYQRLIKVNGGNYFGRFQCNLRVCEPPTSDEVEMDEEVPEPSKTACGGQDNVGAPTDAAIDDTQDGEGTKKKDKNKKKSNKKVFRRLRSLDMRMGAMDTRMSAMEKRLEGVKGELKSIRKYLRRIAKGQLVDPADMRRGKGGDADGGAGSTGDGGYTQGDATDSYPKGDGRGDAAGDGRGDGASDVHDDTIVQNV
Homology
BLAST of MC06g_new0441 vs. NCBI nr
Match: XP_022148308.1 (uncharacterized protein LOC111016993 [Momordica charantia])

HSP 1 Score: 79.7 bits (195), Expect = 2.37e-13
Identity = 52/106 (49.06%), Postives = 69/106 (65.09%), Query Frame = 0

Query: 85  RVCEPPTSDEVEMDEEVPEPSKTACGGQD--------NVGAPTDAAIDDTQDGEGTKKKD 144
           R  E P SDE+E DEEV +  +TA  GQD        N+GAPT A +D  QDGE  + K+
Sbjct: 6   RAIEQPASDELEADEEVHQRLETAFEGQDRNTDRPTDNIGAPTAAEVDRNQDGEAIQNKE 65

Query: 145 KNKKKSNKKVFRRLRSLDMRMGAMDTRMSAMEKRLEGVKGELKSIR 182
           K KKK NKKV +R++ LD       TR++ M+K+LEGV+GELK++R
Sbjct: 66  K-KKKVNKKVLKRIKVLD-------TRVAVMDKKLEGVEGELKAVR 103

BLAST of MC06g_new0441 vs. NCBI nr
Match: XP_022155286.1 (uncharacterized protein LOC111022423 [Momordica charantia])

HSP 1 Score: 73.2 bits (178), Expect = 1.40e-10
Identity = 36/73 (49.32%), Postives = 51/73 (69.86%), Query Frame = 0

Query: 1   MRQNSVACLLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIF--R 60
           +R  SVA L++  GRW+E  VRE+F   EA++IL+ PLP + ++DEIIW MDK GIF  R
Sbjct: 620 VRNFSVAWLMEGRGRWDEIKVRESFIVSEADIILQTPLPSQDKDDEIIWGMDKTGIFSVR 679

Query: 61  SKVHITYQRLIKV 71
           S  H+  Q ++K+
Sbjct: 680 SAYHLGIQLMLKL 692

BLAST of MC06g_new0441 vs. NCBI nr
Match: TXG71894.1 (hypothetical protein EZV62_000473 [Acer yangbiense])

HSP 1 Score: 57.4 bits (137), Expect = 2.11e-05
Identity = 24/55 (43.64%), Postives = 36/55 (65.45%), Query Frame = 0

Query: 6  VACLLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIFRSK 60
          V+ L++  G+WNE+++R NF  EEAE+IL IPL    + D  +W+ +K G F  K
Sbjct: 44 VSTLIESPGKWNESLIRRNFLPEEAELILSIPLSVYLRRDSFLWHFNKDGTFTVK 98

BLAST of MC06g_new0441 vs. NCBI nr
Match: XP_024043202.1 (uncharacterized protein LOC112099905 [Citrus clementina])

HSP 1 Score: 57.0 bits (136), Expect = 2.58e-05
Identity = 26/71 (36.62%), Postives = 43/71 (60.56%), Query Frame = 0

Query: 5  SVACLLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIFRSKVHIT 64
          +V+ L+  +  W   ++++N   ++AE IL IPLP++  EDE+IW+ DKRG +  K    
Sbjct: 6  TVSMLVDNKNNWKTGLIQQNIVKDDAEAILSIPLPRRQSEDEVIWHYDKRGKYIVKS--G 65

Query: 65 YQRLIKVNGGN 75
          YQ  +K+   N
Sbjct: 66 YQVALKLKFPN 74

BLAST of MC06g_new0441 vs. NCBI nr
Match: XP_022149515.1 (uncharacterized protein LOC111017927 [Momordica charantia])

HSP 1 Score: 55.8 bits (133), Expect = 2.93e-05
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 9  LLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIFRSK 60
          L+ +  RWNE+++R +F  EEA++IL IPL   +Q DE+IW  DK+  F  K
Sbjct: 19 LVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVK 70

BLAST of MC06g_new0441 vs. ExPASy TrEMBL
Match: A0A6J1D3R7 (uncharacterized protein LOC111016993 OS=Momordica charantia OX=3673 GN=LOC111016993 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.15e-13
Identity = 52/106 (49.06%), Postives = 69/106 (65.09%), Query Frame = 0

Query: 85  RVCEPPTSDEVEMDEEVPEPSKTACGGQD--------NVGAPTDAAIDDTQDGEGTKKKD 144
           R  E P SDE+E DEEV +  +TA  GQD        N+GAPT A +D  QDGE  + K+
Sbjct: 6   RAIEQPASDELEADEEVHQRLETAFEGQDRNTDRPTDNIGAPTAAEVDRNQDGEAIQNKE 65

Query: 145 KNKKKSNKKVFRRLRSLDMRMGAMDTRMSAMEKRLEGVKGELKSIR 182
           K KKK NKKV +R++ LD       TR++ M+K+LEGV+GELK++R
Sbjct: 66  K-KKKVNKKVLKRIKVLD-------TRVAVMDKKLEGVEGELKAVR 103

BLAST of MC06g_new0441 vs. ExPASy TrEMBL
Match: A0A6J1DRA0 (uncharacterized protein LOC111022423 OS=Momordica charantia OX=3673 GN=LOC111022423 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.76e-11
Identity = 36/73 (49.32%), Postives = 51/73 (69.86%), Query Frame = 0

Query: 1   MRQNSVACLLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIF--R 60
           +R  SVA L++  GRW+E  VRE+F   EA++IL+ PLP + ++DEIIW MDK GIF  R
Sbjct: 620 VRNFSVAWLMEGRGRWDEIKVRESFIVSEADIILQTPLPSQDKDDEIIWGMDKTGIFSVR 679

Query: 61  SKVHITYQRLIKV 71
           S  H+  Q ++K+
Sbjct: 680 SAYHLGIQLMLKL 692

BLAST of MC06g_new0441 vs. ExPASy TrEMBL
Match: A0A5C7IST2 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.02e-05
Identity = 24/55 (43.64%), Postives = 36/55 (65.45%), Query Frame = 0

Query: 6  VACLLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIFRSK 60
          V+ L++  G+WNE+++R NF  EEAE+IL IPL    + D  +W+ +K G F  K
Sbjct: 44 VSTLIESPGKWNESLIRRNFLPEEAELILSIPLSVYLRRDSFLWHFNKDGTFTVK 98

BLAST of MC06g_new0441 vs. ExPASy TrEMBL
Match: A0A6J1D5Y4 (uncharacterized protein LOC111017927 OS=Momordica charantia OX=3673 GN=LOC111017927 PE=4 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.42e-05
Identity = 24/52 (46.15%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 9  LLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIFRSK 60
          L+ +  RWNE+++R +F  EEA++IL IPL   +Q DE+IW  DK+  F  K
Sbjct: 19 LVMKRWRWNESLIRNSFLEEEADIILNIPLSTCNQHDEVIWGPDKKDKFSVK 70

BLAST of MC06g_new0441 vs. ExPASy TrEMBL
Match: A0A2N9EEA9 (CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS967 PE=4 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.05e-05
Identity = 28/68 (41.18%), Postives = 39/68 (57.35%), Query Frame = 0

Query: 9    LLQREGRWNENIVRENFCAEEAEMILKIPLPQKSQEDEIIWNMDKRGIF--RSKVHITYQ 68
            LL+   RWNEN++RENF   EA +I+ I L    + D + WN  K G+F  RS  H+  +
Sbjct: 1360 LLEEPRRWNENLIRENFSEWEAGVIMSIQLRDNRRGDRLFWNETKTGVFSVRSAYHLQLR 1419

Query: 69   RLIKVNGG 74
               +V GG
Sbjct: 1420 HAAEVCGG 1427

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022148308.12.37e-1349.06uncharacterized protein LOC111016993 [Momordica charantia][more]
XP_022155286.11.40e-1049.32uncharacterized protein LOC111022423 [Momordica charantia][more]
TXG71894.12.11e-0543.64hypothetical protein EZV62_000473 [Acer yangbiense][more]
XP_024043202.12.58e-0536.62uncharacterized protein LOC112099905 [Citrus clementina][more]
XP_022149515.12.93e-0546.15uncharacterized protein LOC111017927 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1D3R71.15e-1349.06uncharacterized protein LOC111016993 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A6J1DRA06.76e-1149.32uncharacterized protein LOC111022423 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5C7IST21.02e-0543.64RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 ... [more]
A0A6J1D5Y41.42e-0546.15uncharacterized protein LOC111017927 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A2N9EEA92.05e-0541.18CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS967 ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..159
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 195..258
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 229..249

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0441.1MC06g_new0441.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044237 cellular metabolic process
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:1901363 heterocyclic compound binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0097159 organic cyclic compound binding
molecular_function GO:0016740 transferase activity