MC06g_new0181 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g_new0181 vs. NCBI nr
Match: KAG6590198.1 (hypothetical protein SDJN03_15621, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335 bits (860), Expect = 1.16e-112
Identity = 174/276 (63.04%), Postives = 206/276 (74.64%), Query Frame = 0

Query: 1   MPQFLGSCCSKLVGGVDHALEGEEYIEKGNKLVKWKMPKVPTSKIYRKTTKP--FTLFSS 60
           M  F  SC SK V   DH+LE EEY+EKGN LVKW MP VP  K+Y   TK   FT  + 
Sbjct: 1   MSLFFRSCSSKFVD--DHSLEDEEYVEKGNNLVKWTMPNVPIHKLYEHQTKKKLFTFSTK 60

Query: 61  SDRSIRTSESKISFGNGGGAFKLFDKAPFSYRGKGKYYNIMNIGSVQLGVKTTTRKIPSN 120
            DRSIR +E +ISFGNGGG+FKL+ +AP SY  KGK+Y++MNIG VQ+GVKT TRKIPSN
Sbjct: 61  IDRSIRITEGQISFGNGGGSFKLYKQAPSSYGQKGKFYSVMNIGLVQIGVKTITRKIPSN 120

Query: 121 ASIILCLRDNRVERVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLT-NVFSIHVLI 180
           ASIILC+RDNR+E+V D ++A+VES LGDGPFYFNVFPN+N SL + SL  NV S+HVL+
Sbjct: 121 ASIILCVRDNRIEKVEDSIIAMVESKLGDGPFYFNVFPNMNFSLFEASLIMNVLSVHVLV 180

Query: 181 NGFEQLPEGSQPIVLSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQI--------RESN 240
            GF+QL +GS+PIV+SCRTCYKL+ + FGPEAL+ESPVGKTVFFQTQI            
Sbjct: 181 KGFDQLAKGSEPIVVSCRTCYKLNQTKFGPEALLESPVGKTVFFQTQIVGKLNGGGGGGG 240

Query: 241 DVVQKVTNWEQVQLPPNWPPQLQKRKSLKLVSVENG 265
           DVVQKVT W+QVQLP NWPPQL   K   +    NG
Sbjct: 241 DVVQKVTQWDQVQLPSNWPPQLHIPKFPNVFKRLNG 274

BLAST of MC06g_new0181 vs. NCBI nr
Match: KGN66493.1 (hypothetical protein Csa_007053 [Cucumis sativus])

HSP 1 Score: 311 bits (796), Expect = 6.52e-103
Identity = 163/257 (63.42%), Postives = 199/257 (77.43%), Query Frame = 0

Query: 1   MPQFLGSCCSKLVGGVDHALEGEEYIEKGNKLVKWKMPKVPTSKIYRKTTKP-FTLF-SS 60
           M  F  SC SK   G DH+LE EEY+EKG  LVKWKMP+VP  KIY +  K  F +F S 
Sbjct: 1   MSLFFKSCSSKNFVG-DHSLEEEEYVEKGKSLVKWKMPRVPIHKIYEERRKNHFFIFPSK 60

Query: 61  SDRSIRTSESKISFGNGGGAFKLFDKAPFSY--RGKGKYYNIMNIGSVQLGVKTTTRKIP 120
           +D SIRT+E +ISFGN GG+FKL+ + P SY  R +  ++N MNIG VQ+GVKT T+KIP
Sbjct: 61  NDPSIRTTEGQISFGNEGGSFKLYGQTPSSYCRRRRISFFNTMNIGLVQIGVKTLTKKIP 120

Query: 121 SNASIILCLRDNRVERVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVL 180
            NASIILCLRDNR+E++ D ++ALVES LGDGPFYFNVFPNINLSL   S+TNV S+HVL
Sbjct: 121 PNASIILCLRDNRIEKLEDSLLALVESKLGDGPFYFNVFPNINLSLFFSSITNVLSVHVL 180

Query: 181 INGFEQLPEGSQPIVLSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESND--VVQK 240
           + G +++P+GS PIV++CRTCYKL+ + FG EALIESPVGKTVFFQ +I E +D  VVQK
Sbjct: 181 VKGLDKIPKGSAPIVVTCRTCYKLNQNDFGSEALIESPVGKTVFFQIEIFEDDDDDVVQK 240

Query: 241 VTNWEQVQLPPNWPPQL 251
           VT W QVQLP +WPP+L
Sbjct: 241 VTMWNQVQLPSDWPPRL 256

BLAST of MC06g_new0181 vs. NCBI nr
Match: KGN66494.1 (hypothetical protein Csa_006902 [Cucumis sativus])

HSP 1 Score: 212 bits (540), Expect = 1.42e-64
Identity = 114/236 (48.31%), Postives = 153/236 (64.83%), Query Frame = 0

Query: 8   CCSKLVGGVDHALEGEEYIEKGNKLVKWKMPKVPTSKIYRKTTKPFTLFSSSDRSIRTSE 67
           C S   GG  H+L+ EEYI+KG  L+KWK+PK+PT+KIY+  + PF  FS  D  I+T E
Sbjct: 5   CWSSNFGGGSHSLDSEEYIKKGKNLLKWKIPKIPTTKIYK--SNPFIFFS--DPFIKTKE 64

Query: 68  SKISFGNGGGAFKLFDKAPF--SYRGKGKYYNIMNIGSVQLGVKTTTRKIPSNASIILCL 127
             +   NG   F+L    P   +++ K ++Y  +N+G +Q+GVKT T KIPSNASIILC+
Sbjct: 65  ETMPCENGSQVFRLISGNPMVDNFQEK-RFYTRLNMGMIQIGVKTLTTKIPSNASIILCV 124

Query: 128 RDNRVERVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVLINGFEQLPE 187
            D R +   D ++ LVES L DGP +FN+FPNI + +    L   F +  ++ GFEQLP+
Sbjct: 125 FDTRNDNFEDSILGLVESKLIDGPIFFNIFPNITMPIFHPKLLESFVLIAMVQGFEQLPQ 184

Query: 188 GSQPIVLSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESNDVVQKVTNWEQV 241
           G+ PI L  RTCYKL +S+  P ALIESP GKTVFFQT    S    QKV+ W++V
Sbjct: 185 GTSPISLMWRTCYKLQASAL-PTALIESPQGKTVFFQTNFENSKVADQKVSQWDEV 234

BLAST of MC06g_new0181 vs. NCBI nr
Match: XP_038880673.1 (uncharacterized protein LOC120072292 [Benincasa hispida])

HSP 1 Score: 197 bits (502), Expect = 3.02e-59
Identity = 114/242 (47.11%), Postives = 144/242 (59.50%), Query Frame = 0

Query: 1   MPQFLGSCCSKLVGGVDHALEGEEYIEKGNKLVKWKMPKVPTSKIYRKTTKPFTLFSSSD 60
           M      CCS   GG  HAL+ EEYI+KGN L+KWK+PKVPT+KIY++   PFT FS  D
Sbjct: 1   MATLFKPCCSSNFGGGSHALDSEEYIKKGNNLLKWKIPKVPTTKIYKRN--PFTFFS--D 60

Query: 61  RSIRTSESKISFGNGGGAFKLFDKAPFSYR-GKGKYYNIMNIGSVQLGVKTTTRKIPSNA 120
            SI+T E K+S  NG  AF+L  K P        ++Y  +N+G +Q+GVK  T KIPSNA
Sbjct: 61  PSIKTEEEKMSCENGSQAFRLIAKNPIMASFNDNRFYTTINVGMIQIGVKIMTTKIPSNA 120

Query: 121 SIILCLRDNRVERVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVLING 180
           SIILC+ D+R E   D ++ LVESNLG                                 
Sbjct: 121 SIILCVFDSRNENFEDAILGLVESNLG--------------------------------- 180

Query: 181 FEQLPEGSQPIVLSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESNDVVQKVTNWE 240
           FEQLPEG++PI L  RTCYKL  S+  P AL+ESP GKTV+FQT ++ S   VQKV+ W+
Sbjct: 181 FEQLPEGTRPISLMWRTCYKLQESAL-PNALLESPQGKTVYFQTDLQNSKVAVQKVSKWD 204

BLAST of MC06g_new0181 vs. NCBI nr
Match: KAG6588193.1 (hypothetical protein SDJN03_16758, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 170 bits (431), Expect = 4.71e-48
Identity = 108/252 (42.86%), Postives = 146/252 (57.94%), Query Frame = 0

Query: 7   SCCSKLVGGVDHALEGEEY--IEKG-NKLVKWKMPKVPTSKIYRKTTKPFTLFSSSDRSI 66
           S CSK      H L+ +EY  +EKG + L+ W +P +PTSKIY+  T  FT FS +   +
Sbjct: 8   SSCSKSASS--HTLDTDEYDIVEKGKDDLLTWNIPTLPTSKIYK--TNLFTHFSRNSL-L 67

Query: 67  RTSESKISFGNGGGAFKLFDKAPFSYRGKGKYYNIMNIGSVQLGVKTTTRKIPSNASIIL 126
           +T E      NGGG   L  +    +  + KY  I +IG VQ+GVK  TR  P NA ++L
Sbjct: 68  KTKEGNSPLSNGGGYLNLIREDDDLWHLQNKYRTI-HIGMVQIGVKALTRG-PLNAKVLL 127

Query: 127 CLRDNRVERVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVLINGFEQL 186
           CLRD R   + D ++ +VE+NL DGP YFNVFPNI  SLS   L ++  ++  +  FEQL
Sbjct: 128 CLRDARHRNLQDSLLGMVEANLKDGPLYFNVFPNIVESLSAPRLDDLLFLNAKVREFEQL 187

Query: 187 PEGSQPIVLSCRTCYKLSSSSFGPEALIESPV-GKTVFFQTQIRES-NDVVQKVTNWEQV 246
           PEG + IV+  R CYK+         L+++P  G+T+FFQT    + ND  Q VT W +V
Sbjct: 188 PEGPKNIVVMYRVCYKVLKPGVETRVLLKTPSNGRTIFFQTDFETAMNDFEQGVTVWNEV 247

Query: 247 QLPPNWPPQLQK 253
           QLP NWP    K
Sbjct: 248 QLPENWPTPRSK 252

BLAST of MC06g_new0181 vs. ExPASy TrEMBL
Match: A0A0A0LXM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G614140 PE=4 SV=1)

HSP 1 Score: 311 bits (796), Expect = 3.16e-103
Identity = 163/257 (63.42%), Postives = 199/257 (77.43%), Query Frame = 0

Query: 1   MPQFLGSCCSKLVGGVDHALEGEEYIEKGNKLVKWKMPKVPTSKIYRKTTKP-FTLF-SS 60
           M  F  SC SK   G DH+LE EEY+EKG  LVKWKMP+VP  KIY +  K  F +F S 
Sbjct: 1   MSLFFKSCSSKNFVG-DHSLEEEEYVEKGKSLVKWKMPRVPIHKIYEERRKNHFFIFPSK 60

Query: 61  SDRSIRTSESKISFGNGGGAFKLFDKAPFSY--RGKGKYYNIMNIGSVQLGVKTTTRKIP 120
           +D SIRT+E +ISFGN GG+FKL+ + P SY  R +  ++N MNIG VQ+GVKT T+KIP
Sbjct: 61  NDPSIRTTEGQISFGNEGGSFKLYGQTPSSYCRRRRISFFNTMNIGLVQIGVKTLTKKIP 120

Query: 121 SNASIILCLRDNRVERVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVL 180
            NASIILCLRDNR+E++ D ++ALVES LGDGPFYFNVFPNINLSL   S+TNV S+HVL
Sbjct: 121 PNASIILCLRDNRIEKLEDSLLALVESKLGDGPFYFNVFPNINLSLFFSSITNVLSVHVL 180

Query: 181 INGFEQLPEGSQPIVLSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESND--VVQK 240
           + G +++P+GS PIV++CRTCYKL+ + FG EALIESPVGKTVFFQ +I E +D  VVQK
Sbjct: 181 VKGLDKIPKGSAPIVVTCRTCYKLNQNDFGSEALIESPVGKTVFFQIEIFEDDDDDVVQK 240

Query: 241 VTNWEQVQLPPNWPPQL 251
           VT W QVQLP +WPP+L
Sbjct: 241 VTMWNQVQLPSDWPPRL 256

BLAST of MC06g_new0181 vs. ExPASy TrEMBL
Match: A0A0A0LZS0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G614640 PE=4 SV=1)

HSP 1 Score: 212 bits (540), Expect = 6.89e-65
Identity = 114/236 (48.31%), Postives = 153/236 (64.83%), Query Frame = 0

Query: 8   CCSKLVGGVDHALEGEEYIEKGNKLVKWKMPKVPTSKIYRKTTKPFTLFSSSDRSIRTSE 67
           C S   GG  H+L+ EEYI+KG  L+KWK+PK+PT+KIY+  + PF  FS  D  I+T E
Sbjct: 5   CWSSNFGGGSHSLDSEEYIKKGKNLLKWKIPKIPTTKIYK--SNPFIFFS--DPFIKTKE 64

Query: 68  SKISFGNGGGAFKLFDKAPF--SYRGKGKYYNIMNIGSVQLGVKTTTRKIPSNASIILCL 127
             +   NG   F+L    P   +++ K ++Y  +N+G +Q+GVKT T KIPSNASIILC+
Sbjct: 65  ETMPCENGSQVFRLISGNPMVDNFQEK-RFYTRLNMGMIQIGVKTLTTKIPSNASIILCV 124

Query: 128 RDNRVERVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVLINGFEQLPE 187
            D R +   D ++ LVES L DGP +FN+FPNI + +    L   F +  ++ GFEQLP+
Sbjct: 125 FDTRNDNFEDSILGLVESKLIDGPIFFNIFPNITMPIFHPKLLESFVLIAMVQGFEQLPQ 184

Query: 188 GSQPIVLSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESNDVVQKVTNWEQV 241
           G+ PI L  RTCYKL +S+  P ALIESP GKTVFFQT    S    QKV+ W++V
Sbjct: 185 GTSPISLMWRTCYKLQASAL-PTALIESPQGKTVFFQTNFENSKVADQKVSQWDEV 234

BLAST of MC06g_new0181 vs. ExPASy TrEMBL
Match: A0A5D3D5V1 (Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold204G00750 PE=4 SV=1)

HSP 1 Score: 165 bits (417), Expect = 3.09e-47
Identity = 86/170 (50.59%), Postives = 113/170 (66.47%), Query Frame = 0

Query: 74  NGGGAFKLFDKAPF--SYRGKGKYYNIMNIGSVQLGVKTTTRKIPSNASIILCLRDNRVE 133
           NG  AF+L    P   S++ K ++  ++N+G +Q+GVKT T KIPSNASIILC+ D R +
Sbjct: 5   NGSQAFRLIYDNPILASFQEK-RFCTMLNMGMIQIGVKTLTTKIPSNASIILCVFDTRND 64

Query: 134 RVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVLINGFEQLPEGSQPIV 193
              D ++ LVE+ L DGP +FN+FPNI +SL    L     +  ++ GFEQLP+G+ PI 
Sbjct: 65  NFEDSILGLVEAKLSDGPMFFNIFPNITMSLFHPKLCESLVLIAMVQGFEQLPQGTSPIS 124

Query: 194 LSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESNDVVQKVTNWEQV 241
           L  RTCYKL  S+F P ALIESP GKTVFFQT    S   VQKV+ W++V
Sbjct: 125 LMWRTCYKLQGSAF-PTALIESPQGKTVFFQTDFENSKVAVQKVSEWDEV 172

BLAST of MC06g_new0181 vs. ExPASy TrEMBL
Match: A0A5A7U9X3 (Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1250G00180 PE=4 SV=1)

HSP 1 Score: 162 bits (409), Expect = 4.93e-46
Identity = 85/170 (50.00%), Postives = 112/170 (65.88%), Query Frame = 0

Query: 74  NGGGAFKLFDKAPF--SYRGKGKYYNIMNIGSVQLGVKTTTRKIPSNASIILCLRDNRVE 133
           NG  AF+L    P   S++ K ++  ++N+G +Q+GVKT T KI SNASIILC+ D R +
Sbjct: 5   NGSQAFRLIYDNPILASFQEK-RFCTMLNMGMIQIGVKTLTTKISSNASIILCVFDTRND 64

Query: 134 RVPDRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVLINGFEQLPEGSQPIV 193
              D ++ LVE+ L DGP +FN+FPNI +SL    L     +  ++ GFEQLP+G+ PI 
Sbjct: 65  NFEDSILGLVEAKLSDGPMFFNIFPNITMSLFHPKLCESLVLIAMVQGFEQLPQGTSPIS 124

Query: 194 LSCRTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESNDVVQKVTNWEQV 241
           L  RTCYKL  S+F P ALIESP GKTVFFQT    S   VQKV+ W++V
Sbjct: 125 LMWRTCYKLQGSAF-PTALIESPQGKTVFFQTDFENSKVAVQKVSEWDEV 172

BLAST of MC06g_new0181 vs. ExPASy TrEMBL
Match: A5C0V2 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_001866 PE=4 SV=1)

HSP 1 Score: 129 bits (324), Expect = 6.09e-30
Identity = 85/264 (32.20%), Postives = 136/264 (51.52%), Query Frame = 0

Query: 17  DHALEGEE--YIEKGNKLVKWKMPKVPTSKIYRKTTKPFTLFSSSDRSIRTSESKISFGN 76
           D  +  EE  Y +  N L  WK+PKV   +IY+K T  F  F+  D +I+TSE  +S   
Sbjct: 10  DTVVNSEEINYPKIQNDLDDWKLPKVSNQEIYKKGT--FKFFT--DYTIKTSEMSVSLEQ 69

Query: 77  GGGAFKLFDKAPFSYRGKGKYYNIMNIGSVQLGVKTTTRKIPSNASIILCLRDNRVERVP 136
                +L D      + K   YN ++ G +Q+  K  TR +  N SI++CLRDNR     
Sbjct: 70  DDQVIRLLDNRSID-KHKRDGYNFIHFGMIQVAAKPLTR-LGLNTSIVMCLRDNRHLEYR 129

Query: 137 DRVVALVESNLGDGPFYFNVFPNINLSLSDDSLTNVFSIHVLINGFEQLPEGSQPIVLSC 196
           D ++  V++ L DGP YF  +PN  + + D  + +   +H+  +GF+  P G+ P+ +  
Sbjct: 130 DSIIGAVQAGLNDGPVYFQCYPNFTVRIRDADILDSVVLHIKTHGFKIKP-GNSPVSIIT 189

Query: 197 RTCYKLSSSSFGPEALIESPVGKTVFFQTQIRESND-VVQKVTNWEQVQLPPNW------ 256
           R  YK  ++S G  AL  SP G+T +F + + + +D ++ K   W+ V  P NW      
Sbjct: 190 RFAYKSMNTSVGSGALCTSPKGETTYFHSDMLDKSDFIIPKKILWKDVDFPENWHFANAV 249

Query: 257 PPQLQKRKSLK-LVSVENGIARLV 270
           P   Q+ +S++ +V   +G   LV
Sbjct: 250 PAIAQRSESIEQIVQYPDGGGELV 266