MC06g_new0168 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0168
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionBeta-glucosidase
LocationMC06: 8267892 .. 8274486 (+)
RNA-Seq ExpressionMC06g_new0168
SyntenyMC06g_new0168
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAAAATGTAATCTCCAGCCTCCACTCCACGATTCAAAAACAGTATATTGAAAAAGAAAAAAAATAATTGAAAAGATGTTGAGTTTGTGAGATTTTTGTCTATCTTGACAGTATAAAAAGGGGTATAACGTAACTGAAGCATTACAAACAGTTCTATTCATCCGTCGCCATTCAAAATGGCATTCAAACATGGCTTCCTTTGCTTTGTTCTTCTTCTTGTTGTTCCTCTACTTGCAGTTGCAGACCCCGACGTGGCCCCTTCACATGTCGTTGACATTCTCCACCGGAGCAGTTTTCCCAAAGGCTTTGTTTTTGCCTCAGCATCTTCTGCGTATCAGGTAAAAGAGACCGTTCTTCGTTTATTGTTTCTTGATTTTAATTTTTTTAAAACATAAGCCTTTGGTTACTGTTGCTATTTTCGGTTGATAAACATAAGTCGTCATCGGTTGTTCCGCCGCTATCGACCTTCAGAACTCCAGTCTTCCACCATCCGCATGAATCACATGGAGAGGAGATGGAGAAAACGGGACAGCAGAAAATAACAAGGGCAAAAAATGGGACAAGAGAAAGAAACGAATGGGTAGGAGAGAGGAGAGGAAGAGGGAAGAGAAAGTAGAGAAATTCAACAAAAAAAATAATGGGAGCAAATACATAATTTACCTGAAAATGATGTCACTGATCAGAAAACATAAGTTTTGGGTGGAGACATATCAAAGTGGGCCTATGAGACGAGTTATTTAGAACAAAAACCCGTTTTCATTATTGAAAGTATAAAGTTTTAGATTATTTATTTTATTTTCTGAGCCTGCAAACCTCAAAATCTTTAAAAAATATTAAAGTACATAAACCCACGTTATACGCCTCTTCTTCATTTTCATGTTTCTCACGTACCTTTAATAAAATGGTACCTATTTTTCAAAACGATTTTATTTTTTTCAATTAACATCGTAAAGAAAGTTTTGTAACATTGGTGTGTTTTCTTTTATTTAGTACGAAGGTGATGCATTCAGGGATGGTAAAGGTCTAAGTATCTTTGACAATGCCACTCACCATTTCCCAGGTCTTGCTCTTTATGATTTTTTTCCTCATAATTCTCTCTATTTTGTTTAATTATCACTGTGATGATGTTTTTCAGTATATAATAGGTTTATTTGTTAAAAAGAAAGAAGATTAAAATATGAAGAAACGTCAACCAATTATTTAAGGTCTAAAATATGCAAACTTGACAATTTGAACATAAGTTAACTAGTTAAGACATATATTTTCGACTTAAAAATCGTGAGATTGAATTCACACCCCACATATTGATGTACTATAAAAACATGTCAATCTGAACATAGCTCAATTAATTAAGTAGGTTCGAATTTCTACTATCATTGTTCAACTGAAAAAAAAAGTTATGACATCAAAGAAAAGTTAACAACAAAGATGTAAAAATTCGGAAAAAAGAAATACAAATACAAGGAAAAAAGTAGAAAAAAAAAATCATGCACGTGAATTATTATTATTATTATTTGAGATAGTGGATTATTAATTATTATGAAGTGTATAAATGGAGAATTTTTAAATAAATTAATTTTATCGTGATTTGTAAAATTTGTAGAGAAATTCAACAATGATAACGGAGATGTTGCCATTGATCAATACCATCGCTACAAGGTGTGTGAAAAAAGATTGTATTATTTATTAGTGAAGAAATTTCTCTTATAATATTATGCAAATTTTAGTAATGGTTGTTTCTAACGTAGGAAGATGTTGCAATTATGAAGAAAATGGGCTTAGATGCTTACAGATTCTCAATTGCTTGGTCGAGAGTTTTGCCGAGTAAGAAATTAAAGCTCGTTTAGTAATTATTTCGTATTTTATTGTTTTATTCTTTAGAATTATACTTGTTTGCTGACAATTTTTTTAGCATATTTTATATTGCATAAAAAACTTTGATTCTTAATTTTTCAAATTTAACTTAAATTTTGGAAACGTTTCAAAAAAGTAGACATAAAATAAAGAAATACAGGAATGAAAATAATACTTGCAAATTTATTTATTAAAAAAAAAGAAACTAGAGACAAAGAGTAGGTCGAATCTCCCACCCGTCATGTACTAAAAAAGAATTTTTAAAAATTTTAATAGAAAAATAATTGGAGCTAGTTTTAGTGAAATGGATAATTAATTTTAAGAGTGGTGTTTTTCGCTGTTTTCTTCTAGTGTGTTCTAAGGAATAACTTCTTTCCATAACAAATTAAGTTTTATTTGATCCGTTTGAGGTTTATTTTATTTTGATGTCAAATTTTTTTGAATTCAATTTTTTAATTAACTATTTATAATCACTGTTAATAGAATTATTTTCTCATATGAATTTAAAAGCACAATTTTCAATTCTTATTATACCATCATTTTAATGAGTCAATTTTCAATACAAGTAATCAAGTAGTAAGTTACTATATGCTAAGTCATATTAAATGGTGTGTTTTTAATTAAATGAATGAGCGGTAGATTTATTTCATAAATAAAAACATAAAGTATTTTCAACTACATATTTTAACTTTGCATTATTATGTTTTTGTTATTTTATTTTAATTTTTGTGACCACACATTGACGTGATGAAAAAAAAATAGATGCATAAAAATGAAATTATTAGGAGCCAAATTTAAAAAACACACAAAACATTATAAAACTTCAGTATATATTTAGCCTTTACTAATTAAAAACTATTATGCATAAAGAACATTAAAAAATTTACTATTTTCGAATGAGAAACCATCTCATCAAAGAGATAGTAGGTGAAAAACATACAAAAATACAAAAGAAAAAGAGGTTTAGATAATTCTAGGAGAGAGAGGTTCATGATTAGTCTAATAATTTACTTCTCGCTAATATTTGGTTGGAGAGAAACAATCATATCTTTTCGGAGGTCTCTAAATAAACGGAGAATATATGAAAATAGATGCTTTATCATAGTCGTCTAAACATCCATATTTTTTATGATTATGATATCTCTACTATTTCTCTTAATTGGATGCTATCACGTCTCTTCTTTGTAACATAATTTTTGAATGAAATGATGTCTTTTTTCATAAAAAAGAAAAAAAATTAAAGTAAACATTATTCTCCAATATTATTGTAGATGGAAAACTTAGTGGAGGAGTGAACAAGAAAGGAATTGAGTACTACAACAACCTGATAAATGAACTACTTGCAAACGGTGAGTATTAATTAATGCAATAATTTATTAATCTCTCATTTATTATTTTGCAACCATTTTGTGTTAATTATTCGACAAATATTCACACATTGCTATGAGATTATTTTTGTAGGCATCCAACCCTATGTCACACTTTTTCATTGGGATGTTCCCCAAGCTCTACAAGAGGAATATACAGGCTTCGAGAGTAACAAAATTGTGTAAGCTATTGTTATTTTTTATGACATTTTTTTTATTTTTCACACAATAATAGAAAACAAAATATAATCACGACAAGATATTAAAATTAACTAATTATGACATATTTCACAGAGAAAAAATGGTTTTTAGAATTGTGTTTAAGAATTCTAGGAACGTTTCTCTCAACCAAATTTTAATAATGTTACTCTCAATCAATTGCAGGAATGATTTTCAAGATTATGTCGATCTTTGCTTCAAGGAATTTGGAGATAGAGTAAAGCATTGGATTACCTTAAACGAGCCACATTCCTTCACCCTGATTGTTGATGCTTTTGGTATAATCCCTAGAGACTGTTCTAAGAAACACGATGTACGATTAGATTGTTTGGGTGATGATTCTGGCACCAGAATGTACATTGTTGGTCATAATGAACTTCTTGCTCACGCAGCCGCTGTGAAAGTTTACAAAACTAAATATCAGGTTTTGTCTCACAACTATTCCACCAATTCATATTTACCTTTGCTTTACTATTCAACCCAACATTAGAATTGATCCGAACTTTGACTAGGAAGTAAAATTAAGAAAAATAAATATGTCGTTAAATTAAAGTAAAACTTAATCTTTAGATGTATCATCAATGGTTCTACATGAGTGAGTTTAGAGGTTTGATTTTGATTTGTAAAGTCAAGATCATTTAAACTATGATAATAAGAAATAATAAATGAAATCACACCACATTTTTATCAAATGCTCATGTTAAAAAGTTGTTAAAGTTAGAGTTAATAGGAAAATTTAACACACATATTAAAAATGTCACCATTTTTAAAAATTATAGCATAATTTTGACTTTTTTAATTATATTTTCTTACATTTAAGTTAGACACTAATCTCATTTCATTGTACAATTTGAAAATACATATTTATTTTATGTTTTTCTTGTTACAACAACATATAGGAATTGAAATTCGAACTCACGATCTTTATATATGTCTTAATACATATTTATTTGATGTTATTTACGTAACTATCTCGTATATAAATTTGAACAACGTGAGATTTTGCAGGCACGCCAAAAGGGTGTGATTGGTATTACATTGGATTCGTTTTGGTATGTCCCATATTCAGACAGTGTGGCAGACAAAAAGGCCGCAGAACGAATGCTTGATTTTGGTATTGGTTGGTAAGTAAAGTTCATTGATTACAAATGCATTATACCACCTTATAAAAAAATTTAAAAAGTTTAATATTTGATATATATTTTTGACAACATTAATATTTGATAATCTAAATTAAAATACATGACATGACAGGTTCCTGCACCCACTTACTTATGGAGATTATCCAGCTTCCATGAGAGCGCTTGTAAAGGAGAGATTACCAAAATTTACAAAGGCAGAAAGTAGTTTGATCAAAGGGTCTTTTGATTTTGTTGGAGTAAATTACTACACCGCTAGATTTGCAAAAGACAATCCCAATGCGCCTGGTCCAACGCCACATTACTCAACTGATAGTCGAGCAGACACTTCAAGTAATGTACTTAAACTACGATGATCAAAATTCAAAATTCATCTTTACGTATTCACAAAACACTCAAACAAGTCTCCAGTTCAAAAATGAGATCTGGATAAGATTGATGAGCTGCTAAAAGAGCCAAGGGAACATGTTAAAGATCCCACATTATAATAGACATGAGCTATTTTGCACATTGTCAATTGGTTTTGAAACGGAACCTGTTGAATATTGACACATGACATGATACTTGATACTGATATCTCTTGTAATGTTTTATTTTGGGTTGCAGTGGATCGTGATGGAGTCCTAATTGGTCCGGTGGTAATCATCTTTGTGCAAATTTATTCAATGATGATTATTTATTTATTTTTTATTGCAATGTTTGAAGTCTCAACTAATGATTTATTTTGGTTAACATTGTTACACATAGGCGAATAAGGAATCCTGGCAAGCTCAATATCCACAAGGCCTCCACGAACTATTGGTGTACACGAAGAACAAATACAAAGATCCAAATATATATATCACAGAAAATGGTAACGAAAGAAATCCTTAATTAGGGTATAGTTAATAGTAAAAAATATATTCGTTATTTTAGATAAATGCACATAATTAATTACCTTTTTTATTAATTTCATTGTATGTTGTAGGATTTGTTGATATTGGTGGGACTAAGGCTACGTTACTCAACGACACAGATAGAATTAGATACCATCAACAGCATCTCAGCTCTGTCCTCAAAGCAACCAAGTAAAATCTAAATTACAAAAATTCTCGTGAACTTGGTCTTTTTTGTTTCAAAATATCCACTCCCTTTTAAAAATTTCAATATTATTTTTGTCCTTCGTTTGGAAGTTTGTTTGAGGCTGGAACAATATTAACGACGCTACAACAAATTTGACTTTTAACGTGGTCAATGGTGGATCCATGAATCCATCATTGTTTCTACTCATGCATTTATTTTTACCATCGTTAAAGGTCAAATTTGTTCATGTGTCAGCTAGCTAATTGTTCCCAACAAACTTCCATTAACTACCACATGGTTCCAGATTTACGTTCAAAATATCTAAGTGGAGGTTGCTCAAAGGGTATTTTTTTGAATATTTATAAAAGGTTAAGGATGTAGTTTATATTGTAAGTATATGGTGATCAGTTTGAAGTTTGTCTCAATGATGTTTGCGAAACTTATTATTGCAGGGCTGGTGTAAGGATGAAAGGATACTTTGCATGGTCATTGTTCGATAACTTTGAATGGATATTTGGATATAATTATCGTTTTGGCCTCAACTTTATTGATTACGACAACAACTTGACAAGAATTACCAAGTTGTCAGCCAAATGGTACCAAAATTTCCTCAAGAATTGAAGATGATCCATCAGATCAGATTGTCCCATCTGGCTATTGAAGCTGCTAAGTAAAAGTTAAATAAATTAGGGCCGTTATATTCCAATAACTATATATATTTCTTTTTGTTTCTAAGGAACAAAAGATCAATTTCGTTTGGCGCGCATTCTATTAGAAATCAATGCTTTTTTATTTTATTTTATTTTATTGATGCATACCATTGTGACTGAGGAAGGCCTAGAATATTTGAATGTAAAAAAGTTATAACTGATAGCATTTTAAAAAAAGTTTAAAATATAATTACTATAGCATTTTAAAAATCTTCAATTTTAGTGTTTGGTTCTTCCCTTGGAA

mRNA sequence

ATGGCATTCAAACATGGCTTCCTTTGCTTTGTTCTTCTTCTTGTTGTTCCTCTACTTGCAGTTGCAGACCCCGACGTGGCCCCTTCACATGTCGTTGACATTCTCCACCGGAGCAGTTTTCCCAAAGGCTTTGTTTTTGCCTCAGCATCTTCTGCGTATCAGTACGAAGGTGATGCATTCAGGGATGGTAAAGGTCTAAGTATCTTTGACAATGCCACTCACCATTTCCCAGAGAAATTCAACAATGATAACGGAGATGTTGCCATTGATCAATACCATCGCTACAAGGAAGATGTTGCAATTATGAAGAAAATGGGCTTAGATGCTTACAGATTCTCAATTGCTTGGTCGAGAGTTTTGCCGAATGGAAAACTTAGTGGAGGAGTGAACAAGAAAGGAATTGAGTACTACAACAACCTGATAAATGAACTACTTGCAAACGGCATCCAACCCTATGTCACACTTTTTCATTGGGATGTTCCCCAAGCTCTACAAGAGGAATATACAGGCTTCGAGAGTAACAAAATTGTGAATGATTTTCAAGATTATGTCGATCTTTGCTTCAAGGAATTTGGAGATAGAGTAAAGCATTGGATTACCTTAAACGAGCCACATTCCTTCACCCTGATTGTTGATGCTTTTGGTATAATCCCTAGAGACTGTTCTAAGAAACACGATGTACGATTAGATTGTTTGGGTGATGATTCTGGCACCAGAATGTACATTGTTGGTCATAATGAACTTCTTGCTCACGCAGCCGCTGTGAAAGTTTACAAAACTAAATATCAGGCACGCCAAAAGGGTGTGATTGGTATTACATTGGATTCGTTTTGGTATGTCCCATATTCAGACAGTGTGGCAGACAAAAAGGCCGCAGAACGAATGCTTGATTTTGGTATTGGTTGGTTCCTGCACCCACTTACTTATGGAGATTATCCAGCTTCCATGAGAGCGCTTGTAAAGGAGAGATTACCAAAATTTACAAAGGCAGAAAGTAGTTTGATCAAAGGGTCTTTTGATTTTGTTGGAGTAAATTACTACACCGCTAGATTTGCAAAAGACAATCCCAATGCGCCTGGTCCAACGCCACATTACTCAACTGATAGTCGAGCAGACACTTCAATGGATCGTGATGGAGTCCTAATTGGTCCGGTGGCGAATAAGGAATCCTGGCAAGCTCAATATCCACAAGGCCTCCACGAACTATTGGTGTACACGAAGAACAAATACAAAGATCCAAATATATATATCACAGAAAATGGATTTGTTGATATTGGTGGGACTAAGGCTACGTTACTCAACGACACAGATAGAATTAGATACCATCAACAGCATCTCAGCTCTGTCCTCAAAGCAACCAAGGCTGGTGTAAGGATGAAAGGATACTTTGCATGGTCATTGTTCGATAACTTTGAATGGATATTTGGATATAATTATCGTTTTGGCCTCAACTTTATTGATTACGACAACAACTTGACAAGAATTACCAAGTTGTCAGCCAAATGGTACCAAAATTTCCTCAAGAATTGA

Coding sequence (CDS)

ATGGCATTCAAACATGGCTTCCTTTGCTTTGTTCTTCTTCTTGTTGTTCCTCTACTTGCAGTTGCAGACCCCGACGTGGCCCCTTCACATGTCGTTGACATTCTCCACCGGAGCAGTTTTCCCAAAGGCTTTGTTTTTGCCTCAGCATCTTCTGCGTATCAGTACGAAGGTGATGCATTCAGGGATGGTAAAGGTCTAAGTATCTTTGACAATGCCACTCACCATTTCCCAGAGAAATTCAACAATGATAACGGAGATGTTGCCATTGATCAATACCATCGCTACAAGGAAGATGTTGCAATTATGAAGAAAATGGGCTTAGATGCTTACAGATTCTCAATTGCTTGGTCGAGAGTTTTGCCGAATGGAAAACTTAGTGGAGGAGTGAACAAGAAAGGAATTGAGTACTACAACAACCTGATAAATGAACTACTTGCAAACGGCATCCAACCCTATGTCACACTTTTTCATTGGGATGTTCCCCAAGCTCTACAAGAGGAATATACAGGCTTCGAGAGTAACAAAATTGTGAATGATTTTCAAGATTATGTCGATCTTTGCTTCAAGGAATTTGGAGATAGAGTAAAGCATTGGATTACCTTAAACGAGCCACATTCCTTCACCCTGATTGTTGATGCTTTTGGTATAATCCCTAGAGACTGTTCTAAGAAACACGATGTACGATTAGATTGTTTGGGTGATGATTCTGGCACCAGAATGTACATTGTTGGTCATAATGAACTTCTTGCTCACGCAGCCGCTGTGAAAGTTTACAAAACTAAATATCAGGCACGCCAAAAGGGTGTGATTGGTATTACATTGGATTCGTTTTGGTATGTCCCATATTCAGACAGTGTGGCAGACAAAAAGGCCGCAGAACGAATGCTTGATTTTGGTATTGGTTGGTTCCTGCACCCACTTACTTATGGAGATTATCCAGCTTCCATGAGAGCGCTTGTAAAGGAGAGATTACCAAAATTTACAAAGGCAGAAAGTAGTTTGATCAAAGGGTCTTTTGATTTTGTTGGAGTAAATTACTACACCGCTAGATTTGCAAAAGACAATCCCAATGCGCCTGGTCCAACGCCACATTACTCAACTGATAGTCGAGCAGACACTTCAATGGATCGTGATGGAGTCCTAATTGGTCCGGTGGCGAATAAGGAATCCTGGCAAGCTCAATATCCACAAGGCCTCCACGAACTATTGGTGTACACGAAGAACAAATACAAAGATCCAAATATATATATCACAGAAAATGGATTTGTTGATATTGGTGGGACTAAGGCTACGTTACTCAACGACACAGATAGAATTAGATACCATCAACAGCATCTCAGCTCTGTCCTCAAAGCAACCAAGGCTGGTGTAAGGATGAAAGGATACTTTGCATGGTCATTGTTCGATAACTTTGAATGGATATTTGGATATAATTATCGTTTTGGCCTCAACTTTATTGATTACGACAACAACTTGACAAGAATTACCAAGTTGTCAGCCAAATGGTACCAAAATTTCCTCAAGAATTGA

Protein sequence

MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAFRDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYRFGLNFIDYDNNLTRITKLSAKWYQNFLKN
Homology
BLAST of MC06g_new0168 vs. ExPASy Swiss-Prot
Match: Q5Z9Z0 (Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.4e-159
Identity = 274/502 (54.58%), Postives = 353/502 (70.32%), Query Frame = 0

Query: 13  LLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAFRDGKGLSIFDNA 72
           LL++ L+A +    +     +++ RS FP+ F F +ASSAYQYEG     G+G SI+D  
Sbjct: 6   LLLLLLMASSTSSRSEMKAGEVIRRSQFPEDFFFGTASSAYQYEGAVREGGRGPSIWDTF 65

Query: 73  THHFPEKF-NNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSGGVNK 132
           TH+ PEK  N  NGD+AID YHRYKEDV IMK +GL+AYRFS++W R+LPNGKLSGGVN 
Sbjct: 66  THNHPEKIANGSNGDIAIDSYHRYKEDVGIMKGLGLNAYRFSVSWPRILPNGKLSGGVNL 125

Query: 133 KGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLCFKEF 192
           +GI+YYNNLI+EL++ G++P+VTLFHWD PQAL+++Y GF SN IV DF+DY D+CF+EF
Sbjct: 126 EGIKYYNNLIDELISKGVEPFVTLFHWDSPQALEQQYGGFLSNLIVEDFRDYADICFREF 185

Query: 193 GDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSGTRMYIVGHNELLA 252
           GDRVK+WIT NEP SF++   + GI+ P  CS +   +  C   DSG   YIV HN+LLA
Sbjct: 186 GDRVKYWITFNEPWSFSIGGYSNGILAPGRCSSQG--KSGCSKGDSGREPYIVAHNQLLA 245

Query: 253 HAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIGWFLHPLTYG 312
           HAA V++Y+ KYQ  QKG IGI + S W +PY DS  DK A +R LDF  GWF+ PLT G
Sbjct: 246 HAAVVQIYREKYQGGQKGKIGIAIVSNWMIPYEDSKEDKHATKRALDFMYGWFMDPLTKG 305

Query: 313 DYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHYSTDSR 372
           DYP SMR LV  RLP+FTK +S  I GSFDF+G+NYYTAR+ +           YSTDS 
Sbjct: 306 DYPVSMRTLVGNRLPRFTKEQSKAINGSFDFIGLNYYTARYIQGTKQDSNSHKSYSTDSL 365

Query: 373 ADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVDIGGTKA 432
            +  ++R+G  IGP A   SW   YP+G+ ELL+YTK  Y +P IYITENG  ++     
Sbjct: 366 TNERVERNGTDIGPKAG-SSWLYIYPKGIEELLLYTKRTYNNPTIYITENGVDEVNNENL 425

Query: 433 TL---LNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYRFGLNFID 492
           +L   L DT RI +++QHL  V +A + GV ++GYFAWSLFDNFEW+ GY+ RFG+N+ID
Sbjct: 426 SLKEALIDTTRIEFYRQHLFHVQRALRQGVDVRGYFAWSLFDNFEWMDGYSVRFGINYID 485

Query: 493 YDNNLTRITKLSAKWYQNFLKN 510
           Y + L R  K S++W QNFL N
Sbjct: 486 YKDGLKRYPKRSSQWLQNFLHN 504

BLAST of MC06g_new0168 vs. ExPASy Swiss-Prot
Match: B8AVF0 (Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 8.7e-154
Identity = 271/501 (54.09%), Postives = 342/501 (68.26%), Query Frame = 0

Query: 12  LLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAFRDGKGLSIFDN 71
           LLL   LLAV       S     + R SFPKGF+F +ASS+YQYEG A   G+G SI+D 
Sbjct: 11  LLLTFLLLAVVASGAYNSAGEPPVSRRSFPKGFIFGTASSSYQYEGGAAEGGRGPSIWDT 70

Query: 72  ATHHFPEKF-NNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSGGVN 131
            TH  PEK  +  NGDVA D YH YKEDV +MK MG+DAYRFSI+W+R+LPNG L GGVN
Sbjct: 71  FTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVN 130

Query: 132 KKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLCFKE 191
           K+GI+YYNNLINELL+ G+QP++TLFHWD PQAL+++Y GF S  I+NDF+DY ++CFKE
Sbjct: 131 KEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKE 190

Query: 192 FGDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSGTRMYIVGHNELL 251
           FGDRVK+WIT NEP +F     A G+  P  CS     + +C   DSG   Y   H++LL
Sbjct: 191 FGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWE--KGNCSVGDSGREPYTACHHQLL 250

Query: 252 AHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIGWFLHPLTY 311
           AHA  V++YK KYQA QKG IGITL S W+VP+S S ++  AA+R +DF  GWF+ PL  
Sbjct: 251 AHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNNDAAKRAIDFMFGWFMDPLIR 310

Query: 312 GDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHYSTDS 371
           GDYP SMR LV  RLP+FTK +S L+KG+FDF+G+NYYTA +A + P + G    Y+TDS
Sbjct: 311 GDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDS 370

Query: 372 RADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVDIGGTK 431
           RA+ +  R+G+ IGP A    W   YPQG  +LL+Y K  Y +P +YITENG  +     
Sbjct: 371 RANLTGVRNGIPIGPQA-ASPWLYVYPQGFRDLLLYVKENYGNPTVYITENGVDEFNNKT 430

Query: 432 ATL---LNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYRFGLNFI 491
             L   L D  RI Y+ +HL S+L A + G  +KGYFAWSL DNFEW  GY  RFG+NF+
Sbjct: 431 LPLQEALKDDARIEYYHKHLLSLLSAIRDGANVKGYFAWSLLDNFEWSNGYTVRFGINFV 490

Query: 492 DYDNNLTRITKLSAKWYQNFL 508
           DY++   R  K SA W++ FL
Sbjct: 491 DYNDGRKRYPKNSAHWFKKFL 508

BLAST of MC06g_new0168 vs. ExPASy Swiss-Prot
Match: Q7XKV4 (Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2)

HSP 1 Score: 543.1 bits (1398), Expect = 3.3e-153
Identity = 270/501 (53.89%), Postives = 341/501 (68.06%), Query Frame = 0

Query: 12  LLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAFRDGKGLSIFDN 71
           LLL   LLAV             + R SFPKGF+F +ASS+YQYEG A   G+G SI+D 
Sbjct: 11  LLLTFLLLAVVASGAYNGAGEPPVSRRSFPKGFIFGTASSSYQYEGGAAEGGRGPSIWDT 70

Query: 72  ATHHFPEKF-NNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSGGVN 131
            TH  PEK  +  NGDVA D YH YKEDV +MK MG+DAYRFSI+W+R+LPNG L GGVN
Sbjct: 71  FTHQHPEKIADRSNGDVASDSYHLYKEDVRLMKDMGMDAYRFSISWTRILPNGSLRGGVN 130

Query: 132 KKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLCFKE 191
           K+GI+YYNNLINELL+ G+QP++TLFHWD PQAL+++Y GF S  I+NDF+DY ++CFKE
Sbjct: 131 KEGIKYYNNLINELLSKGVQPFITLFHWDSPQALEDKYNGFLSPNIINDFKDYAEICFKE 190

Query: 192 FGDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSGTRMYIVGHNELL 251
           FGDRVK+WIT NEP +F     A G+  P  CS     + +C   DSG   Y   H++LL
Sbjct: 191 FGDRVKNWITFNEPWTFCSNGYATGLFAPGRCSPWE--KGNCSVGDSGREPYTACHHQLL 250

Query: 252 AHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIGWFLHPLTY 311
           AHA  V++YK KYQA QKG IGITL S W+VP+S S ++  AA+R +DF  GWF+ PL  
Sbjct: 251 AHAETVRLYKAKYQALQKGKIGITLVSHWFVPFSRSKSNDDAAKRAIDFMFGWFMDPLIR 310

Query: 312 GDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHYSTDS 371
           GDYP SMR LV  RLP+FTK +S L+KG+FDF+G+NYYTA +A + P + G    Y+TDS
Sbjct: 311 GDYPLSMRGLVGNRLPQFTKEQSKLVKGAFDFIGLNYYTANYADNLPPSNGLNNSYTTDS 370

Query: 372 RADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVDIGGTK 431
           RA+ +  R+G+ IGP A    W   YPQG  +LL+Y K  Y +P +YITENG  +     
Sbjct: 371 RANLTGVRNGIPIGPQA-ASPWLYVYPQGFRDLLLYVKENYGNPTVYITENGVDEFNNKT 430

Query: 432 ATL---LNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYRFGLNFI 491
             L   L D  RI Y+ +HL S+L A + G  +KGYFAWSL DNFEW  GY  RFG+NF+
Sbjct: 431 LPLQEALKDDARIEYYHKHLLSLLSAIRDGANVKGYFAWSLLDNFEWSNGYTVRFGINFV 490

Query: 492 DYDNNLTRITKLSAKWYQNFL 508
           DY++   R  K SA W++ FL
Sbjct: 491 DYNDGRKRYPKNSAHWFKKFL 508

BLAST of MC06g_new0168 vs. ExPASy Swiss-Prot
Match: Q7XKV2 (Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2)

HSP 1 Score: 542.3 bits (1396), Expect = 5.7e-153
Identity = 270/501 (53.89%), Postives = 342/501 (68.26%), Query Frame = 0

Query: 14  LVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAFRDGKGLSIFDNAT 73
           +++PLL V    VA S     + R SFP+GF+F +ASS+YQYEG A   G+G SI+D  T
Sbjct: 13  ILLPLLLV----VAVSGEPPPISRRSFPEGFIFGTASSSYQYEGGAREGGRGPSIWDTFT 72

Query: 74  HHFPEKF-NNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSGGVNKK 133
           H  P+K  +  NGDVA D YH YKEDV IMK MG+DAYRFSI+W+R+LPNG LSGG+N++
Sbjct: 73  HQHPDKIADKSNGDVAADSYHLYKEDVRIMKDMGVDAYRFSISWTRILPNGSLSGGINRE 132

Query: 134 GIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLCFKEFG 193
           GI YYNNLINELL  G+QP+VTLFHWD PQAL+++Y GF S  I+ND+++Y + CFKEFG
Sbjct: 133 GISYYNNLINELLLKGVQPFVTLFHWDSPQALEDKYNGFLSPNIINDYKEYAETCFKEFG 192

Query: 194 DRVKHWITLNEPHSFTLIVDAFG--IIPRDCSKKHDVRLDCLGDDSGTRMYIVGHNELLA 253
           DRVKHWIT NEP SF +   A G    P  CS       +C   DSG   Y   H++LLA
Sbjct: 193 DRVKHWITFNEPLSFCVAGYASGGMFAPGRCSPWEG---NCSAGDSGREPYTACHHQLLA 252

Query: 254 HAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIGWFLHPLTYG 313
           HA  V++YK KYQ  QKG IGITL S W+VP+S S ++  AA R LDF +GWF+ PL  G
Sbjct: 253 HAETVRLYKEKYQVLQKGKIGITLVSNWFVPFSRSKSNIDAARRALDFMLGWFMDPLIRG 312

Query: 314 DYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHYSTDSR 373
           +YP SMR LV+ RLP+FTK +S LIKGSFDF+G+NYYT+ +A   P + G    YSTD+R
Sbjct: 313 EYPLSMRELVRNRLPQFTKEQSELIKGSFDFIGLNYYTSNYAGSLPPSNGLNNSYSTDAR 372

Query: 374 ADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVDIGGTKA 433
           A+ +  R+G+ IGP A    W   YPQG  EL++Y K  Y +P IYITENG  +      
Sbjct: 373 ANLTAVRNGIPIGPQA-ASPWLYIYPQGFRELVLYVKENYGNPTIYITENGVDEFNNKTL 432

Query: 434 TL---LNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYRFGLNFID 493
            L   L D  RI Y+ +HL S+L A + G  +KGYFAWSL DNFEW  GY  RFG+NF+D
Sbjct: 433 PLQEALKDDTRIDYYHKHLLSLLSAIRDGANVKGYFAWSLLDNFEWSNGYTVRFGINFVD 492

Query: 494 YDNNLTRITKLSAKWYQNFLK 509
           Y++   R  K+SA W++ FL+
Sbjct: 493 YNDGAKRYPKMSAHWFKEFLQ 505

BLAST of MC06g_new0168 vs. ExPASy Swiss-Prot
Match: Q42707 (Furostanol glycoside 26-O-beta-glucosidase OS=Hellenia speciosa OX=49577 GN=F26G PE=1 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 6.3e-152
Identity = 271/490 (55.31%), Postives = 340/490 (69.39%), Query Frame = 0

Query: 22  ADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAFRDGKGLSIFDNATHHFPEKF- 81
           A P   PS VV  L RSSFP+GF+F +AS+AYQ EG     G+G SI+D  TH  PEK  
Sbjct: 80  ATPTAVPSKVV--LGRSSFPRGFIFGAASAAYQVEGAWNEGGRGPSIWDTFTHDHPEKIA 139

Query: 82  NNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSGGVNKKGIEYYNNL 141
           ++ NGD A D Y +YKEDV ++K +GLD+YRFSI+WSR+LP G L GG+N++GI+YYN+L
Sbjct: 140 DHSNGDKATDSYKKYKEDVKLLKDLGLDSYRFSISWSRILPKGTLQGGINQEGIQYYNDL 199

Query: 142 INELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLCFKEFGDRVKHWIT 201
           INELL NGI+P VTLFHWDVPQAL++ Y GF S++IVNDF+DY D+CFKEFGDRVKHWIT
Sbjct: 200 INELLKNGIRPMVTLFHWDVPQALEDSYKGFRSSEIVNDFKDYADICFKEFGDRVKHWIT 259

Query: 202 LNEPHSFTLIVDAFG-IIPRDCSKKHDVRLDCLGDDSGTRMYIVGHNELLAHAAAVKVYK 261
           LNEP S + +  AFG   P  CS  +     C   DS    Y V HN LLAHA AVK+Y+
Sbjct: 260 LNEPWSLSTMGYAFGRHAPGRCSTWY----GCPAGDSANEPYEVTHNLLLAHANAVKIYR 319

Query: 262 TKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIGWFLHPLTYGDYPASMRAL 321
             Y+A Q G IGITL+S WY PYS S  D +AA R LDF  GW++ PL  GDYP  MRAL
Sbjct: 320 DNYKATQNGEIGITLNSLWYEPYSKSHEDVEAATRALDFMFGWYMDPLVNGDYPFIMRAL 379

Query: 322 VKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHYS-TDSRADTSMDRD 381
           V++RLP FT AES LIKGS+DF+G+NYYT+ +A+  P     TP  S  DS  + S +++
Sbjct: 380 VRDRLPFFTHAESELIKGSYDFIGINYYTSNYAQHAPVTEDHTPDNSYFDSYVNQSGEKN 439

Query: 382 GVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVDIGGTKATLLNDTDR 441
           GV IGP+  + SW   YP+GL ELL+Y K +Y +P IYITENG  ++   K   L+D +R
Sbjct: 440 GVPIGPL--QGSWIYFYPRGLKELLLYVKRRYCNPKIYITENGTAEVEKEKGVPLHDPER 499

Query: 442 IRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYRFGLNFIDYDNNLTRITKL 501
             Y   HL+ VL+A + GVR+KG+F W+L DNFEW  GY  RFGL +IDYD +  R  K 
Sbjct: 500 KEYLTYHLAQVLQAIREGVRVKGHFTWALTDNFEWDKGYTERFGLIYIDYDKDFNRQPKD 559

Query: 502 SAKWYQNFLK 509
           S KW+  FL+
Sbjct: 560 STKWFSKFLR 561

BLAST of MC06g_new0168 vs. NCBI nr
Match: XP_022154076.1 (cyanogenic beta-glucosidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60
           MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF
Sbjct: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60

Query: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120
           RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL
Sbjct: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120

Query: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180
           PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF
Sbjct: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180

Query: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240
           QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM
Sbjct: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240

Query: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300
           YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI
Sbjct: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300

Query: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360
           GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG
Sbjct: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360

Query: 361 PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420
           PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN
Sbjct: 361 PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420

Query: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480
           GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR
Sbjct: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480

Query: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           FGLNFIDYDNNLTRITKLSAKWYQNFLKN
Sbjct: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 509

BLAST of MC06g_new0168 vs. NCBI nr
Match: XP_022154077.1 (cyanogenic beta-glucosidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 508/509 (99.80%), Postives = 508/509 (99.80%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60
           MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF
Sbjct: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60

Query: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120
           RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL
Sbjct: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120

Query: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180
           PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF
Sbjct: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180

Query: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240
           QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM
Sbjct: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240

Query: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300
           YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI
Sbjct: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300

Query: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360
           GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG
Sbjct: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360

Query: 361 PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420
           PTPHYSTDSRADTSMDRDGVLIGP ANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN
Sbjct: 361 PTPHYSTDSRADTSMDRDGVLIGP-ANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420

Query: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480
           GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR
Sbjct: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480

Query: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           FGLNFIDYDNNLTRITKLSAKWYQNFLKN
Sbjct: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 508

BLAST of MC06g_new0168 vs. NCBI nr
Match: XP_008450457.1 (PREDICTED: cyanogenic beta-glucosidase-like [Cucumis melo])

HSP 1 Score: 701 bits (1810), Expect = 1.80e-249
Identity = 348/513 (67.84%), Postives = 402/513 (78.36%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHV-VDILHRSSFPKGFVFASASSAYQYEGDA 60
           MA K   +   L+L V L  +A     PSH+ +D++ RSSFPKGFVF SASSAYQ+EG A
Sbjct: 1   MASKQSHIFLSLVLFVGLFVIAKAKEEPSHIPIDLVRRSSFPKGFVFGSASSAYQFEGAA 60

Query: 61  FRDGKGLSIFDNATHHFPEK-FNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSR 120
           F DGK  SI+DN TH  PEK +++  GDVA+DQYHRYKEDVA+MKKMG DAYRFSIAWSR
Sbjct: 61  FEDGKKPSIWDNYTHQHPEKIYDHSTGDVAVDQYHRYKEDVALMKKMGFDAYRFSIAWSR 120

Query: 121 VLPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVN 180
           VLP GKLSGGVNKKGI YYNNLINELLA GIQPYVTLFHWD PQAL++EY GF  ++IVN
Sbjct: 121 VLPKGKLSGGVNKKGIRYYNNLINELLAKGIQPYVTLFHWDTPQALEDEYGGFLGHQIVN 180

Query: 181 DFQDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSG 240
           DF+D+V++CFKEFGDRVKHWITLNEP SF +   A G + P  CS       +CLG +SG
Sbjct: 181 DFRDFVEICFKEFGDRVKHWITLNEPWSFAMGGYAQGALAPGRCSSWQP--FNCLGGNSG 240

Query: 241 TRMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLD 300
           T  YIVGH+++LAHAAAVK+YKTKYQARQKGVIGITL S WY PYS+S ADKKAA R LD
Sbjct: 241 TEPYIVGHHQILAHAAAVKLYKTKYQARQKGVIGITLVSIWYTPYSNSEADKKAAIRSLD 300

Query: 301 FGIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPN 360
           F +GWFLHPLTYGDYP  MR LVKERLPKFTKAE +LIKGS DF+G+NYYTA +AKDNPN
Sbjct: 301 FALGWFLHPLTYGDYPPIMRELVKERLPKFTKAEVALIKGSIDFLGLNYYTANYAKDNPN 360

Query: 361 APGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYI 420
           APGP P Y TD RAD S DR+GV IGP  N  SW A YP+G  +LL+YTK KYK+P IYI
Sbjct: 361 APGPQPSYLTDYRADQSTDRNGVSIGPKFNATSWLAVYPKGFKDLLIYTKTKYKNPIIYI 420

Query: 421 TENGFVDIGGTKAT-LLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFG 480
           TENG++DI G     +L D  R++YH  HL ++ +A +AGVR+KGYFAWS  DNFEW  G
Sbjct: 421 TENGYLDIEGPPLKEMLMDRRRVKYHHDHLMALKEAIQAGVRVKGYFAWSFLDNFEWASG 480

Query: 481 YNYRFGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           Y  RFGL +IDY NNL RI KLSAKW++NFLKN
Sbjct: 481 YVIRFGLTYIDYKNNLKRIPKLSAKWFENFLKN 511

BLAST of MC06g_new0168 vs. NCBI nr
Match: XP_022960706.1 (cyanogenic beta-glucosidase-like [Cucurbita moschata])

HSP 1 Score: 701 bits (1808), Expect = 3.49e-249
Identity = 345/511 (67.51%), Postives = 404/511 (79.06%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60
           MAFKHG    + ++++  L VA  D+AP   +D++ RSSF K FVF SAS+AYQ+EG AF
Sbjct: 1   MAFKHGHSFLISMVLLLFLIVAKADLAPRRPIDVVRRSSFSKNFVFGSASAAYQFEGAAF 60

Query: 61  RDGKGLSIFDNATHHFPEK-FNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRV 120
            DGKG SI+DN TH  PEK +++ NGDVA+DQYHRYKEDVAI+KKMG +AYRFSIAWSRV
Sbjct: 61  EDGKGPSIWDNFTHQHPEKIYDHSNGDVALDQYHRYKEDVAILKKMGFNAYRFSIAWSRV 120

Query: 121 LPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVND 180
           LP GKLSGGVNKKGIEYYNNLINELL NGIQP+VTLFHWD PQAL++EY GF   +IVND
Sbjct: 121 LPKGKLSGGVNKKGIEYYNNLINELLRNGIQPFVTLFHWDTPQALEDEYGGFLGRQIVND 180

Query: 181 FQDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGI-IPRDCSKKHDVRLDCLGDDSGT 240
           ++DY +LCFKEFGDRVKHWITLNEP SF++   A G   P  CS     +  CLG DSGT
Sbjct: 181 YRDYAELCFKEFGDRVKHWITLNEPWSFSMGGYAQGANAPGRCSSWQPYK--CLGGDSGT 240

Query: 241 RMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDF 300
             YIVGH++LLAHAAAVKVYKTKYQA QKGVIGITL + WY P S+S ADKKAA R LDF
Sbjct: 241 EPYIVGHHQLLAHAAAVKVYKTKYQAYQKGVIGITLVTVWYTPNSNSEADKKAAIRALDF 300

Query: 301 GIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNA 360
            +GW+LHP+TYGDYP  MR LVKERLPKF+ AES+ IKGS DF+G+NYYTA +AKDNPNA
Sbjct: 301 ALGWYLHPITYGDYPPIMRELVKERLPKFSAAESASIKGSLDFLGLNYYTANYAKDNPNA 360

Query: 361 PGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYIT 420
           PGP P Y TD RAD S DR+GV IGP  ++ SW A YPQGL ELL+YTK KY DP IYIT
Sbjct: 361 PGPQPSYLTDYRADLSTDRNGVSIGPKFSEVSWLAVYPQGLKELLIYTKTKYNDPFIYIT 420

Query: 421 ENGFVDIGG-TKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGY 480
           ENG+++I G T   L+ND  R++YH  HL ++ +A K+GV++KGYFAWS  DNFEW  GY
Sbjct: 421 ENGYLEIEGPTFEELINDKARVKYHHDHLYNLNEAIKSGVKVKGYFAWSFLDNFEWSSGY 480

Query: 481 NYRFGLNFIDYDNNLTRITKLSAKWYQNFLK 508
             RFGLN+IDY NNL RI KLSAKW++NFLK
Sbjct: 481 AVRFGLNYIDYKNNLKRIPKLSAKWFENFLK 509

BLAST of MC06g_new0168 vs. NCBI nr
Match: XP_011660111.2 (cyanogenic beta-glucosidase [Cucumis sativus] >KGN66451.1 hypothetical protein Csa_007041 [Cucumis sativus])

HSP 1 Score: 700 bits (1806), Expect = 7.29e-249
Identity = 343/513 (66.86%), Postives = 403/513 (78.56%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHV-VDILHRSSFPKGFVFASASSAYQYEGDA 60
           MA K    C   +L+V L+ +A  +  PSH+ +D++ RSSFPKGFVF +ASSAYQ+EG A
Sbjct: 1   MASKQSHSCLSFVLLVVLIVIAKANGEPSHIPIDVVRRSSFPKGFVFGTASSAYQFEGGA 60

Query: 61  FRDGKGLSIFDNATHHFPEK-FNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSR 120
           F DGK  SI+DN TH  PEK +++ NGDVA++QYHRYKEDVA+MKKMG DAYRFSIAWSR
Sbjct: 61  FEDGKRPSIWDNYTHQHPEKIYDHSNGDVAVNQYHRYKEDVALMKKMGFDAYRFSIAWSR 120

Query: 121 VLPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVN 180
           VLP GKLSGGVNKKGI+YYNNLINELLA GIQPYVTLFHWD PQAL++EY GF  ++IVN
Sbjct: 121 VLPKGKLSGGVNKKGIQYYNNLINELLAKGIQPYVTLFHWDTPQALEDEYGGFLGHQIVN 180

Query: 181 DFQDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSG 240
           DF+D+ ++CFKEFGDRVKHWITLNEP SF +   A G + P  CS       +CLG +SG
Sbjct: 181 DFRDFAEVCFKEFGDRVKHWITLNEPWSFAMGGYAQGALAPGRCSSWQP--FNCLGGNSG 240

Query: 241 TRMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLD 300
           T  YIVGHN++LAHAAAVKVYKTKYQA QKGVIGITL S WY PYS+S ADKKAA R LD
Sbjct: 241 TEPYIVGHNQILAHAAAVKVYKTKYQAHQKGVIGITLVSIWYTPYSNSEADKKAANRSLD 300

Query: 301 FGIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPN 360
           F +GWFLHPLTYGDYP  MR LVKERLPKFT+AE++LIKGS DF+G+NYYT  +AKDNP 
Sbjct: 301 FALGWFLHPLTYGDYPPIMRELVKERLPKFTRAEAALIKGSMDFLGLNYYTTNYAKDNPK 360

Query: 361 APGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYI 420
           APGP P Y TD RAD S DR+GV IGP  N  SW A YP+G  +LL++TK KYK+P IYI
Sbjct: 361 APGPQPSYLTDYRADLSTDRNGVSIGPKFNATSWLAVYPKGFKDLLIHTKTKYKNPIIYI 420

Query: 421 TENGFVDIGGTKAT-LLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFG 480
           TENG++DI G     +L D  R++YH  HL ++ +A +AGVR+KGYFAWS  DNFEW  G
Sbjct: 421 TENGYLDIEGPPLKEMLMDRRRVKYHHDHLMALKEAMQAGVRVKGYFAWSFLDNFEWASG 480

Query: 481 YNYRFGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           Y  RFGL +IDY NNL RI KLSAKW++NFLKN
Sbjct: 481 YVIRFGLTYIDYKNNLKRIPKLSAKWFENFLKN 511

BLAST of MC06g_new0168 vs. ExPASy TrEMBL
Match: A0A6J1DMM4 (cyanogenic beta-glucosidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021422 PE=3 SV=1)

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60
           MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF
Sbjct: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60

Query: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120
           RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL
Sbjct: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120

Query: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180
           PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF
Sbjct: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180

Query: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240
           QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM
Sbjct: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240

Query: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300
           YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI
Sbjct: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300

Query: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360
           GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG
Sbjct: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360

Query: 361 PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420
           PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN
Sbjct: 361 PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420

Query: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480
           GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR
Sbjct: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480

Query: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           FGLNFIDYDNNLTRITKLSAKWYQNFLKN
Sbjct: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 509

BLAST of MC06g_new0168 vs. ExPASy TrEMBL
Match: A0A6J1DKP5 (cyanogenic beta-glucosidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021422 PE=3 SV=1)

HSP 1 Score: 1049 bits (2712), Expect = 0.0
Identity = 508/509 (99.80%), Postives = 508/509 (99.80%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60
           MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF
Sbjct: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60

Query: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120
           RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL
Sbjct: 61  RDGKGLSIFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVL 120

Query: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180
           PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF
Sbjct: 121 PNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDF 180

Query: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240
           QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM
Sbjct: 181 QDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGIIPRDCSKKHDVRLDCLGDDSGTRM 240

Query: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300
           YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI
Sbjct: 241 YIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGI 300

Query: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360
           GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG
Sbjct: 301 GWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 360

Query: 361 PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420
           PTPHYSTDSRADTSMDRDGVLIGP ANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN
Sbjct: 361 PTPHYSTDSRADTSMDRDGVLIGP-ANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 420

Query: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480
           GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR
Sbjct: 421 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 480

Query: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           FGLNFIDYDNNLTRITKLSAKWYQNFLKN
Sbjct: 481 FGLNFIDYDNNLTRITKLSAKWYQNFLKN 508

BLAST of MC06g_new0168 vs. ExPASy TrEMBL
Match: A0A1S3BP89 (cyanogenic beta-glucosidase-like OS=Cucumis melo OX=3656 GN=LOC103492052 PE=3 SV=1)

HSP 1 Score: 701 bits (1810), Expect = 8.71e-250
Identity = 348/513 (67.84%), Postives = 402/513 (78.36%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHV-VDILHRSSFPKGFVFASASSAYQYEGDA 60
           MA K   +   L+L V L  +A     PSH+ +D++ RSSFPKGFVF SASSAYQ+EG A
Sbjct: 1   MASKQSHIFLSLVLFVGLFVIAKAKEEPSHIPIDLVRRSSFPKGFVFGSASSAYQFEGAA 60

Query: 61  FRDGKGLSIFDNATHHFPEK-FNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSR 120
           F DGK  SI+DN TH  PEK +++  GDVA+DQYHRYKEDVA+MKKMG DAYRFSIAWSR
Sbjct: 61  FEDGKKPSIWDNYTHQHPEKIYDHSTGDVAVDQYHRYKEDVALMKKMGFDAYRFSIAWSR 120

Query: 121 VLPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVN 180
           VLP GKLSGGVNKKGI YYNNLINELLA GIQPYVTLFHWD PQAL++EY GF  ++IVN
Sbjct: 121 VLPKGKLSGGVNKKGIRYYNNLINELLAKGIQPYVTLFHWDTPQALEDEYGGFLGHQIVN 180

Query: 181 DFQDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSG 240
           DF+D+V++CFKEFGDRVKHWITLNEP SF +   A G + P  CS       +CLG +SG
Sbjct: 181 DFRDFVEICFKEFGDRVKHWITLNEPWSFAMGGYAQGALAPGRCSSWQP--FNCLGGNSG 240

Query: 241 TRMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLD 300
           T  YIVGH+++LAHAAAVK+YKTKYQARQKGVIGITL S WY PYS+S ADKKAA R LD
Sbjct: 241 TEPYIVGHHQILAHAAAVKLYKTKYQARQKGVIGITLVSIWYTPYSNSEADKKAAIRSLD 300

Query: 301 FGIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPN 360
           F +GWFLHPLTYGDYP  MR LVKERLPKFTKAE +LIKGS DF+G+NYYTA +AKDNPN
Sbjct: 301 FALGWFLHPLTYGDYPPIMRELVKERLPKFTKAEVALIKGSIDFLGLNYYTANYAKDNPN 360

Query: 361 APGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYI 420
           APGP P Y TD RAD S DR+GV IGP  N  SW A YP+G  +LL+YTK KYK+P IYI
Sbjct: 361 APGPQPSYLTDYRADQSTDRNGVSIGPKFNATSWLAVYPKGFKDLLIYTKTKYKNPIIYI 420

Query: 421 TENGFVDIGGTKAT-LLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFG 480
           TENG++DI G     +L D  R++YH  HL ++ +A +AGVR+KGYFAWS  DNFEW  G
Sbjct: 421 TENGYLDIEGPPLKEMLMDRRRVKYHHDHLMALKEAIQAGVRVKGYFAWSFLDNFEWASG 480

Query: 481 YNYRFGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           Y  RFGL +IDY NNL RI KLSAKW++NFLKN
Sbjct: 481 YVIRFGLTYIDYKNNLKRIPKLSAKWFENFLKN 511

BLAST of MC06g_new0168 vs. ExPASy TrEMBL
Match: A0A6J1H8C8 (cyanogenic beta-glucosidase-like OS=Cucurbita moschata OX=3662 GN=LOC111461424 PE=3 SV=1)

HSP 1 Score: 701 bits (1808), Expect = 1.69e-249
Identity = 345/511 (67.51%), Postives = 404/511 (79.06%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60
           MAFKHG    + ++++  L VA  D+AP   +D++ RSSF K FVF SAS+AYQ+EG AF
Sbjct: 1   MAFKHGHSFLISMVLLLFLIVAKADLAPRRPIDVVRRSSFSKNFVFGSASAAYQFEGAAF 60

Query: 61  RDGKGLSIFDNATHHFPEK-FNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRV 120
            DGKG SI+DN TH  PEK +++ NGDVA+DQYHRYKEDVAI+KKMG +AYRFSIAWSRV
Sbjct: 61  EDGKGPSIWDNFTHQHPEKIYDHSNGDVALDQYHRYKEDVAILKKMGFNAYRFSIAWSRV 120

Query: 121 LPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVND 180
           LP GKLSGGVNKKGIEYYNNLINELL NGIQP+VTLFHWD PQAL++EY GF   +IVND
Sbjct: 121 LPKGKLSGGVNKKGIEYYNNLINELLRNGIQPFVTLFHWDTPQALEDEYGGFLGRQIVND 180

Query: 181 FQDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGI-IPRDCSKKHDVRLDCLGDDSGT 240
           ++DY +LCFKEFGDRVKHWITLNEP SF++   A G   P  CS     +  CLG DSGT
Sbjct: 181 YRDYAELCFKEFGDRVKHWITLNEPWSFSMGGYAQGANAPGRCSSWQPYK--CLGGDSGT 240

Query: 241 RMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDF 300
             YIVGH++LLAHAAAVKVYKTKYQA QKGVIGITL + WY P S+S ADKKAA R LDF
Sbjct: 241 EPYIVGHHQLLAHAAAVKVYKTKYQAYQKGVIGITLVTVWYTPNSNSEADKKAAIRALDF 300

Query: 301 GIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNA 360
            +GW+LHP+TYGDYP  MR LVKERLPKF+ AES+ IKGS DF+G+NYYTA +AKDNPNA
Sbjct: 301 ALGWYLHPITYGDYPPIMRELVKERLPKFSAAESASIKGSLDFLGLNYYTANYAKDNPNA 360

Query: 361 PGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYIT 420
           PGP P Y TD RAD S DR+GV IGP  ++ SW A YPQGL ELL+YTK KY DP IYIT
Sbjct: 361 PGPQPSYLTDYRADLSTDRNGVSIGPKFSEVSWLAVYPQGLKELLIYTKTKYNDPFIYIT 420

Query: 421 ENGFVDIGG-TKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGY 480
           ENG+++I G T   L+ND  R++YH  HL ++ +A K+GV++KGYFAWS  DNFEW  GY
Sbjct: 421 ENGYLEIEGPTFEELINDKARVKYHHDHLYNLNEAIKSGVKVKGYFAWSFLDNFEWSSGY 480

Query: 481 NYRFGLNFIDYDNNLTRITKLSAKWYQNFLK 508
             RFGLN+IDY NNL RI KLSAKW++NFLK
Sbjct: 481 AVRFGLNYIDYKNNLKRIPKLSAKWFENFLK 509

BLAST of MC06g_new0168 vs. ExPASy TrEMBL
Match: A0A0A0M2N7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G611280 PE=3 SV=1)

HSP 1 Score: 700 bits (1806), Expect = 3.53e-249
Identity = 343/513 (66.86%), Postives = 403/513 (78.56%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHV-VDILHRSSFPKGFVFASASSAYQYEGDA 60
           MA K    C   +L+V L+ +A  +  PSH+ +D++ RSSFPKGFVF +ASSAYQ+EG A
Sbjct: 1   MASKQSHSCLSFVLLVVLIVIAKANGEPSHIPIDVVRRSSFPKGFVFGTASSAYQFEGGA 60

Query: 61  FRDGKGLSIFDNATHHFPEK-FNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSR 120
           F DGK  SI+DN TH  PEK +++ NGDVA++QYHRYKEDVA+MKKMG DAYRFSIAWSR
Sbjct: 61  FEDGKRPSIWDNYTHQHPEKIYDHSNGDVAVNQYHRYKEDVALMKKMGFDAYRFSIAWSR 120

Query: 121 VLPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVN 180
           VLP GKLSGGVNKKGI+YYNNLINELLA GIQPYVTLFHWD PQAL++EY GF  ++IVN
Sbjct: 121 VLPKGKLSGGVNKKGIQYYNNLINELLAKGIQPYVTLFHWDTPQALEDEYGGFLGHQIVN 180

Query: 181 DFQDYVDLCFKEFGDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSG 240
           DF+D+ ++CFKEFGDRVKHWITLNEP SF +   A G + P  CS       +CLG +SG
Sbjct: 181 DFRDFAEVCFKEFGDRVKHWITLNEPWSFAMGGYAQGALAPGRCSSWQP--FNCLGGNSG 240

Query: 241 TRMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLD 300
           T  YIVGHN++LAHAAAVKVYKTKYQA QKGVIGITL S WY PYS+S ADKKAA R LD
Sbjct: 241 TEPYIVGHNQILAHAAAVKVYKTKYQAHQKGVIGITLVSIWYTPYSNSEADKKAANRSLD 300

Query: 301 FGIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPN 360
           F +GWFLHPLTYGDYP  MR LVKERLPKFT+AE++LIKGS DF+G+NYYT  +AKDNP 
Sbjct: 301 FALGWFLHPLTYGDYPPIMRELVKERLPKFTRAEAALIKGSMDFLGLNYYTTNYAKDNPK 360

Query: 361 APGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYI 420
           APGP P Y TD RAD S DR+GV IGP  N  SW A YP+G  +LL++TK KYK+P IYI
Sbjct: 361 APGPQPSYLTDYRADLSTDRNGVSIGPKFNATSWLAVYPKGFKDLLIHTKTKYKNPIIYI 420

Query: 421 TENGFVDIGGTKAT-LLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFG 480
           TENG++DI G     +L D  R++YH  HL ++ +A +AGVR+KGYFAWS  DNFEW  G
Sbjct: 421 TENGYLDIEGPPLKEMLMDRRRVKYHHDHLMALKEAMQAGVRVKGYFAWSFLDNFEWASG 480

Query: 481 YNYRFGLNFIDYDNNLTRITKLSAKWYQNFLKN 509
           Y  RFGL +IDY NNL RI KLSAKW++NFLKN
Sbjct: 481 YVIRFGLTYIDYKNNLKRIPKLSAKWFENFLKN 511

BLAST of MC06g_new0168 vs. TAIR 10
Match: AT2G44480.1 (beta glucosidase 17 )

HSP 1 Score: 495.4 bits (1274), Expect = 5.6e-140
Identity = 240/514 (46.69%), Postives = 349/514 (67.90%), Query Frame = 0

Query: 1   MAFKHGFLCFVLLLVVPLLAVADPDVAPSHVVDILHRSSFPKGFVFASASSAYQYEGDAF 60
           MA K  F+  ++ ++  +  +   D +   +   L RSSFP+ F F +ASSAYQ EG A 
Sbjct: 1   MAIKSIFIIIIISIITSISELYALDPSFLRLSTSLQRSSFPQDFRFGAASSAYQSEGAAN 60

Query: 61  RDGKGLSIFDNATHHFPEKFNN-DNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRV 120
            DG+  SI+D  T  +PEK ++  NGDVA + Y+R+KEDVA MK++GLD++RFSI+WSR+
Sbjct: 61  VDGREPSIWDTFTKQYPEKISDGSNGDVADEFYYRFKEDVAHMKEIGLDSFRFSISWSRI 120

Query: 121 LPNGKLSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVND 180
           LP G ++GGVN+ GI +YN+LINEL++NGI+P VTLFHWD PQAL++EY GF + +IV D
Sbjct: 121 LPRGTVAGGVNQAGINFYNHLINELISNGIRPLVTLFHWDTPQALEDEYGGFLNPQIVKD 180

Query: 181 FQDYVDLCFKEFGDRVKHWITLNEPHSFTLI-VDAFGIIPRDCSKKHDVRLDCLGDDSGT 240
           F +YVD+CFKEFGDRVK WIT+NEP+ F ++  +   I P  CS       +C   +S T
Sbjct: 181 FVEYVDICFKEFGDRVKEWITINEPNMFAVLGYNVGNIAPGRCSSYVQ---NCTVGNSAT 240

Query: 241 RMYIVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDF 300
             Y+V H  +L+HAA V++Y+ KYQ+   G IG+T+ ++W +P  ++ A ++AA+R LDF
Sbjct: 241 EPYLVAHYLILSHAATVQLYREKYQSFHGGTIGMTIQTYWMIPKYNTPACREAAKRALDF 300

Query: 301 GIGWFLHPLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNA 360
             GWF  P+TYGDYP +MR LV  RLPKFTK +S +++GSFDF G+NYYT+R+ +D    
Sbjct: 301 FFGWFADPITYGDYPKTMRELVGNRLPKFTKKQSKMVRGSFDFFGLNYYTSRYVEDVMFY 360

Query: 361 PGPTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYIT 420
                 Y+TDSR + + +++GV +G   + + W    P+G  ++L+Y K+K+++P I +T
Sbjct: 361 ANTNLSYTTDSRVNQTTEKNGVPVGEPTSAD-WLFICPEGFQDVLLYIKSKFQNPVILVT 420

Query: 421 ENGFVDIGGTKATL---LNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIF 480
           ENG         ++   LND  +I+YHQ HL+++L+A   G  ++GY+ WSL D+FEW F
Sbjct: 421 ENGMPSENDKSLSVNIALNDEAKIKYHQLHLTALLEAVSQGADVRGYYIWSLMDDFEWEF 480

Query: 481 GYNYRFGLNFIDYDNNLTRITKLSAKWYQNFLKN 510
           GY YR+GL ++D+ + L R  K SA WY +FL N
Sbjct: 481 GYKYRYGLVYVDFQDGLKRHLKSSALWYHHFLSN 510

BLAST of MC06g_new0168 vs. TAIR 10
Match: AT3G60130.1 (beta glucosidase 16 )

HSP 1 Score: 495.4 bits (1274), Expect = 5.6e-140
Identity = 255/508 (50.20%), Postives = 336/508 (66.14%), Query Frame = 0

Query: 7   FLCFVLLLVVPLLAVADPDVAPSHVV-DILHRSSFPKGFVFASASSAYQYEGDAFRDGKG 66
           FL  +LL+ +  + V+    A  H     L R+ FP+ FVF SA+SAYQ EG A  DG+G
Sbjct: 5   FLSLLLLITLACIGVS----AKKHSTRPRLRRNDFPQDFVFGSATSAYQCEGAAHEDGRG 64

Query: 67  LSIFDNATHHFPEK-FNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGK 126
            SI+D+ +  FPEK  +  NG +A D Y+ YKEDV ++ ++G DAYRFSI+WSR+LP G 
Sbjct: 65  PSIWDSFSEKFPEKIMDGSNGSIADDSYNLYKEDVNLLHQIGFDAYRFSISWSRILPRGT 124

Query: 127 LSGGVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYV 186
           L GG+N+ GIEYYNNLIN+L++ G++P+VTLFHWD+P AL+  Y G   ++ VNDF+DY 
Sbjct: 125 LKGGINQAGIEYYNNLINQLISKGVKPFVTLFHWDLPDALENAYGGLLGDEFVNDFRDYA 184

Query: 187 DLCFKEFGDRVKHWITLNEPHSFTLIVDAF---GIIPRDCSKKHDVRLDCLGDDSGTRMY 246
           +LCF++FGDRVK W TLNEP  +T++ + +      P  CS  +  + DCLG D+ T  Y
Sbjct: 185 ELCFQKFGDRVKQWTTLNEP--YTMVHEGYITGQKAPGRCSNFY--KPDCLGGDAATEPY 244

Query: 247 IVGHNELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIG 306
           IVGHN LLAH  AVKVY+ KYQA QKG IGI L++ W+ PYSDS AD+ AA R   F   
Sbjct: 245 IVGHNLLLAHGVAVKVYREKYQATQKGEIGIALNTAWHYPYSDSYADRLAATRATAFTFD 304

Query: 307 WFLHPLTYGDYPASMRALVKE-RLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPG 366
           +F+ P+ YG YP  M + VK+ RLP FT  ES ++KGS+DF+GVNYY++ +AKD P A  
Sbjct: 305 YFMEPIVYGRYPIEMVSHVKDGRLPTFTPEESEMLKGSYDFIGVNYYSSLYAKDVPCAT- 364

Query: 367 PTPHYSTDSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITEN 426
                +TDS      +R+GV IGP A  + W   YP+G+ +LL++ K +Y DP +YITEN
Sbjct: 365 ENITMTTDSCVSLVGERNGVPIGPAAGSD-WLLIYPKGIRDLLLHAKFRYNDPVLYITEN 424

Query: 427 GFVDIGGTKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYR 486
           G VD        LND  RI Y+  HL  V  A   GV +KGYFAWSL DNFEW  GY  R
Sbjct: 425 G-VDEANIGKIFLNDDLRIDYYAHHLKMVSDAISIGVNVKGYFAWSLMDNFEWSEGYTVR 484

Query: 487 FGLNFIDYDNNLTRITKLSAKWYQNFLK 509
           FGL F+D+++   R  K SAKW++  LK
Sbjct: 485 FGLVFVDFEDGRKRYLKKSAKWFRRLLK 501

BLAST of MC06g_new0168 vs. TAIR 10
Match: AT5G24540.1 (beta glucosidase 31 )

HSP 1 Score: 495.0 bits (1273), Expect = 7.4e-140
Identity = 252/510 (49.41%), Postives = 333/510 (65.29%), Query Frame = 0

Query: 12  LLLVVPLLAVADPDVAPSHVVDI----LHRSSFPKGFVFASASSAYQYEGDAFRDGKGLS 71
           L+ +V  L VA  DVA    +      L+R SFP  F F  ASSAYQYEG     G+ LS
Sbjct: 5   LIALVITLCVASWDVAQGRSLRFSTTPLNRYSFPPHFDFGVASSAYQYEGAVEEGGRSLS 64

Query: 72  IFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSG 131
           I+DN TH FPE+ N DNGDVA+D YHRYKED+ ++K+M +D++RFS++WSR+LP+GKLS 
Sbjct: 65  IWDNFTHAFPERTNMDNGDVAVDFYHRYKEDIKLIKEMNMDSFRFSLSWSRILPSGKLSD 124

Query: 132 GVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLC 191
           GVNK+G+++Y NLI+EL+ NGI+P+VT++HWD+PQAL +EY  F S +I++DF++Y   C
Sbjct: 125 GVNKEGVQFYKNLIDELIENGIKPFVTIYHWDIPQALDDEYGSFLSPRIIDDFRNYARFC 184

Query: 192 FKEFGDRVKHWITLNEPHSFTLI-VDAFGIIPRDCSKKHDVRLDCLGDDSGTRMYIVGHN 251
           F+EFGD+V  W T NEP+ +++   DA       CSK   V   C+  DSGT  Y+V H+
Sbjct: 185 FQEFGDKVSMWTTFNEPYVYSVSGYDAGNKAMGRCSKW--VNSLCIAGDSGTEPYLVSHH 244

Query: 252 ELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPY-SDSVADKKAAERMLDFGIGWFLH 311
            LLAHAAAV+ ++   +  Q   IGI L  +W+ PY S S ADK+A ER L F IGW L 
Sbjct: 245 LLLAHAAAVEEFRKCDKISQDSKIGIVLSPYWFEPYDSASNADKEAVERALAFNIGWHLS 304

Query: 312 PLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHY 371
           PL +GDYP +++     RLP FTK +S ++K SFDF+GVNYYTARF   + N     P +
Sbjct: 305 PLVFGDYPETIKISAGNRLPSFTKEQSMMVKNSFDFIGVNYYTARFVAHDLNVDISRPRF 364

Query: 372 STDSRAD---TSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGF 431
            TD       T+   D + +     K  W   YP+GL ++L Y KNKY +P IYITENGF
Sbjct: 365 MTDQHLQYKLTNRTGDTISLESDGTKILW--SYPEGLRKILNYIKNKYNNPTIYITENGF 424

Query: 432 VDIGG---TKATLLNDTDRIRYHQQHLSSVLKA-TKAGVRMKGYFAWSLFDNFEWIFGYN 491
            D      T+  +L DT RI YHQ+HL  + KA T+ G  +KGYF WSL DNFEW  GY 
Sbjct: 425 DDYENGTVTREEILEDTKRIEYHQKHLQELQKAITEDGCDVKGYFTWSLLDNFEWEHGYA 484

Query: 492 YRFGLNFIDYDNNLTRITKLSAKWYQNFLK 509
            RFGL ++DY N L R  K SA W+++FL+
Sbjct: 485 VRFGLYYVDYKNGLQRHAKHSAMWFKHFLE 510

BLAST of MC06g_new0168 vs. TAIR 10
Match: AT5G24550.1 (beta glucosidase 32 )

HSP 1 Score: 488.8 bits (1257), Expect = 5.3e-138
Identity = 244/508 (48.03%), Postives = 333/508 (65.55%), Query Frame = 0

Query: 12  LLLVVPLLAVADPDVAPSHVVDI----LHRSSFPKGFVFASASSAYQYEGDAFRDGKGLS 71
           L+ +V  + VA  D A    +      L+R SFP  F F  ASSAYQYEG     G+  S
Sbjct: 5   LIALVITICVASWDSAQGRSLRFSTTPLNRYSFPPHFDFGVASSAYQYEGAVEEGGRSPS 64

Query: 72  IFDNATHHFPEKFNNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSG 131
           I+DN TH FPE+ N DNGDVA+D YHRYK+D+ ++K+M +D++RFS++WSR+LP+GKLS 
Sbjct: 65  IWDNFTHAFPERTNMDNGDVAVDFYHRYKDDIKLIKEMNMDSFRFSLSWSRILPSGKLSD 124

Query: 132 GVNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLC 191
           GVNK+G+++Y NLI+EL+ NGI+P+VT++HWD+PQAL +EY  F S +I++DF+++   C
Sbjct: 125 GVNKEGVQFYKNLIDELIKNGIKPFVTIYHWDIPQALDDEYGSFLSPRIIDDFRNFARFC 184

Query: 192 FKEFGDRVKHWITLNEPHSFTLI-VDAFGIIPRDCSKKHDVRLDCLGDDSGTRMYIVGHN 251
           F+EFGD+V  W T NEP+ +++   DA       CSK   V   C+  DSGT  Y+V HN
Sbjct: 185 FQEFGDKVSMWTTFNEPYVYSVSGYDAGNKAIGRCSKW--VNSLCIAGDSGTEPYLVSHN 244

Query: 252 ELLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYS-DSVADKKAAERMLDFGIGWFLH 311
            LLAHAAAV+ ++   +  Q   IGI L  +W+ PY  DS +DK+A ER L F IGW L 
Sbjct: 245 LLLAHAAAVEEFRKCDKISQDAKIGIVLSPYWFEPYDIDSESDKEAVERALVFNIGWHLS 304

Query: 312 PLTYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHY 371
           PL +GDYP +++     RLP FTK +S +++ SFDF+G+NYYTARF   + +     P +
Sbjct: 305 PLVFGDYPETIKTTAGNRLPSFTKEQSMMLQNSFDFIGINYYTARFVAHDLHVDLSRPRF 364

Query: 372 STDSRADTSM-DRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVD 431
           +TD      + +R G  I   ++       YP+GL +LL Y KNKY +P IYITENGF D
Sbjct: 365 TTDQHLQYKLTNRSGDHISSESDGTKILWSYPEGLRKLLNYIKNKYNNPTIYITENGFDD 424

Query: 432 IGG---TKATLLNDTDRIRYHQQHLSSVLKA-TKAGVRMKGYFAWSLFDNFEWIFGYNYR 491
                 T+  ++ DT RI YHQ HL  + KA T+ G  +KGYF WSL DNFEW  GY  R
Sbjct: 425 YENGSVTREEIIEDTKRIEYHQNHLQQLQKAITEDGCNVKGYFTWSLLDNFEWEHGYAVR 484

Query: 492 FGLNFIDYDNNLTRITKLSAKWYQNFLK 509
           FGL ++DY N L+R  K SAKW+++FL+
Sbjct: 485 FGLYYVDYKNGLSRHAKNSAKWFKHFLQ 510

BLAST of MC06g_new0168 vs. TAIR 10
Match: AT2G44450.1 (beta glucosidase 15 )

HSP 1 Score: 488.4 bits (1256), Expect = 6.9e-138
Identity = 243/500 (48.60%), Postives = 330/500 (66.00%), Query Frame = 0

Query: 12  LLLVVPLLAVADPDVAPSH--VVDILHRSSFPKGFVFASASSAYQYEGDAFRDGKGLSIF 71
           L L+V L+ +A  DV  ++      L RS FP+ F+F SA+SAYQ EG A  DG+G SI+
Sbjct: 6   LSLLVVLIVLASNDVLANNNSSTPKLRRSDFPEDFIFGSATSAYQVEGGAHEDGRGPSIW 65

Query: 72  DNATHHFPEKF-NNDNGDVAIDQYHRYKEDVAIMKKMGLDAYRFSIAWSRVLPNGKLSGG 131
           D  +  +PEK  +  NG VA + YH YKEDVA++ ++G +AYRFSI+WSR+LP G L GG
Sbjct: 66  DTFSEKYPEKIKDGSNGSVADNSYHLYKEDVALLHQIGFNAYRFSISWSRILPRGNLKGG 125

Query: 132 VNKKGIEYYNNLINELLANGIQPYVTLFHWDVPQALQEEYTGFESNKIVNDFQDYVDLCF 191
           +N+ GI+YYNNLINELL+ GI+P+ T+FHWD PQAL++ Y GF   +IVNDF+DY D+CF
Sbjct: 126 INQAGIDYYNNLINELLSKGIKPFATMFHWDTPQALEDAYGGFRGAEIVNDFRDYADICF 185

Query: 192 KEFGDRVKHWITLNEPHSFTLIVDAFGII-PRDCSKKHDVRLDCLGDDSGTRMYIVGHNE 251
           K FGDRVKHW+TLNEP +        G++ P  CSK      +C   +  T  YIVGHN 
Sbjct: 186 KNFGDRVKHWMTLNEPLTVVQQGYVAGVMAPGRCSK--FTNPNCTDGNGATEPYIVGHNL 245

Query: 252 LLAHAAAVKVYKTKYQARQKGVIGITLDSFWYVPYSDSVADKKAAERMLDFGIGWFLHPL 311
           +L+H AAV+VY+ KY+A Q+G +GI L++ W +PY++S  D+ AA R + F   +F+ PL
Sbjct: 246 ILSHGAAVQVYREKYKASQQGQVGIALNAGWNLPYTESPKDRLAAARAMAFTFDYFMEPL 305

Query: 312 TYGDYPASMRALVKERLPKFTKAESSLIKGSFDFVGVNYYTARFAKDNPNAPGPTPHYST 371
             G YP  M   VK RLP FT  +S ++KGS+DF+G+NYY++ +AKD P +      +S 
Sbjct: 306 VTGKYPVDMVNNVKGRLPIFTAQQSKMLKGSYDFIGINYYSSTYAKDVPCSTKDVTMFS- 365

Query: 372 DSRADTSMDRDGVLIGPVANKESWQAQYPQGLHELLVYTKNKYKDPNIYITENGFVDIGG 431
           D  A  + +RDGV IGP A  + W   YP+G+ +L++Y K K+KDP +YITENG  D   
Sbjct: 366 DPCASVTGERDGVPIGPKAASD-WLLIYPKGIRDLVLYAKYKFKDPVMYITENG-RDEFS 425

Query: 432 TKATLLNDTDRIRYHQQHLSSVLKATKAGVRMKGYFAWSLFDNFEWIFGYNYRFGLNFID 491
           T    L D DRI Y+ +HL  V  A   G  +KG+FAWSL DNFEW  GY  RFGL ++D
Sbjct: 426 TNKIFLKDGDRIDYYARHLEMVQDAISVGANVKGFFAWSLLDNFEWAMGYTVRFGLVYVD 485

Query: 492 YDNNLTRITKLSAKWYQNFL 508
           + +   R  K SA+W++  L
Sbjct: 486 FKDGCKRYPKKSAEWFRKLL 500

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5Z9Z02.4e-15954.58Beta-glucosidase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU24 PE=2 SV=1[more]
B8AVF08.7e-15454.09Beta-glucosidase 12 OS=Oryza sativa subsp. indica OX=39946 GN=BGLU12 PE=3 SV=1[more]
Q7XKV43.3e-15353.89Beta-glucosidase 12 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU12 PE=1 SV=2[more]
Q7XKV25.7e-15353.89Beta-glucosidase 13 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU13 PE=2 SV=2[more]
Q427076.3e-15255.31Furostanol glycoside 26-O-beta-glucosidase OS=Hellenia speciosa OX=49577 GN=F26G... [more]
Match NameE-valueIdentityDescription
XP_022154076.10.0100.00cyanogenic beta-glucosidase-like isoform X1 [Momordica charantia][more]
XP_022154077.10.099.80cyanogenic beta-glucosidase-like isoform X2 [Momordica charantia][more]
XP_008450457.11.80e-24967.84PREDICTED: cyanogenic beta-glucosidase-like [Cucumis melo][more]
XP_022960706.13.49e-24967.51cyanogenic beta-glucosidase-like [Cucurbita moschata][more]
XP_011660111.27.29e-24966.86cyanogenic beta-glucosidase [Cucumis sativus] >KGN66451.1 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
A0A6J1DMM40.0100.00cyanogenic beta-glucosidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1DKP50.099.80cyanogenic beta-glucosidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A1S3BP898.71e-25067.84cyanogenic beta-glucosidase-like OS=Cucumis melo OX=3656 GN=LOC103492052 PE=3 SV... [more]
A0A6J1H8C81.69e-24967.51cyanogenic beta-glucosidase-like OS=Cucurbita moschata OX=3662 GN=LOC111461424 P... [more]
A0A0A0M2N73.53e-24966.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G611280 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G44480.15.6e-14046.69beta glucosidase 17 [more]
AT3G60130.15.6e-14050.20beta glucosidase 16 [more]
AT5G24540.17.4e-14049.41beta glucosidase 31 [more]
AT5G24550.15.3e-13848.03beta glucosidase 32 [more]
AT2G44450.16.9e-13848.60beta glucosidase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001360Glycoside hydrolase family 1PRINTSPR00131GLHYDRLASE1coord: 415..423
score: 61.04
coord: 338..352
score: 47.38
coord: 435..446
score: 47.11
coord: 456..473
score: 59.41
coord: 480..492
score: 41.13
IPR001360Glycoside hydrolase family 1PFAMPF00232Glyco_hydro_1coord: 37..508
e-value: 3.9E-153
score: 510.1
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 29..508
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 23..509
e-value: 3.9E-197
score: 657.5
NoneNo IPR availablePIRSRPIRSR617736-1PIRSR617736-1coord: 37..509
e-value: 1.2E-142
score: 473.5
NoneNo IPR availablePANTHERPTHR10353:SF272SUBFAMILY NOT NAMEDcoord: 29..508
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 32..509

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0168.1MC06g_new0168.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006611 protein export from nucleus
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005643 nuclear pore
molecular_function GO:0008422 beta-glucosidase activity
molecular_function GO:0005049 nuclear export signal receptor activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds