MC06g_new0117 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0117
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description2-alkenal reductase (NADP(+)-dependent)-like
LocationMC06: 4995531 .. 4998819 (+)
RNA-Seq ExpressionMC06g_new0117
SyntenyMC06g_new0117
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAACACCTTCATCGCCATTAAACATCTCATCAATGGCTTCCCACTGCAAACACACTTCCAACTCAACTCCCAACCTCTGAATCTGTGTGTGAAGCCTGCTTCTGGTGACATCCTCCTCAAAAATCTGTGTGTCTCCATTGACCCATATCAGATCAACCGTATGAACATCCACTCCTCTTCCCACAACGTTTCATCTGCTACACCTTCTATCGTTCCCGGACAGGTAATCGGTTTTCCTTTTTGGTTAGAATGAGACGGCCATGATACTCGTCGCTGCTCTATCGTTGAATCCAAATTCGAAATCTAGCATGCTCCTCTTGTAACTACAAATTTAAGTTGGGACGTTTTTTAAGATCTTACGCTAATATTTTAAGCACACTAGGAATGGAACATTAAGGACTCATTTGTTAAGGTTCTTATTTCTTATCTTTTTTTTTCCTTTTTCCAATTTTGTTAGAAGATATCCAAACATTTGACAACTATATATTTAGGTTTCCTTGTTTTTCAAATTTTTAAAAACAAATTTAAAAAACAATTTTTTTTTTGTTCTCCTTTCTAATTGGAGAGTACTTCCTTTGGTTTGTCCTTTACCACTTCTCTCTCCCTTAGTGGTAGCAAAAACAGACACGTAGTTTCTGTTGGAAAAATTAATATGGCTTCTAAGCACCACGCTATTTAGGAATACAGAATGAAAAACCTCACTAACCTCAAGAGAACGGACTGCCGACAATGCTCTATAAATCAAGTTGTCTTTTCTTCCTCTGCGAACAAAACACCACTGCCGCCCCTTTTTCCTTTTGATGCAACAACACCACCACAGCCACCACTCTCTTATTTTTTTTATCTCTCCCGGTAAAGTAGCATAAAAATCCTCCATCATGTCTATTAATATTAGGTCATTAAACCTAATGAAAACAAAGCTATGGCCTTTAGTGTGACTATTTTGGCCCAATAACTTTAATAATTATTTTGTCTTTCATCATATCTCAATAGTTTTCAATAATATTGTATTGTTTTATCATTCTTAAATCCATAGAATTGAGATCACACAATTCTATTCTTCTTTATTAAAAAAGTGAAAAAAATAAAGAGAGACCCTCTTTAGATATCCACTTTTTCAAGGTTGACTGACCAATCAATAGAACTGTCGAGCTACAATAGAGCTTCTTCTTCTCTAGTTCACCACTCCTATATGAAGAGTAATTTTCATTAAGCAGATGGTATTTAGAACGTTACCAAAGCCAAAGGGTGCCTAATCTTTTGAATTTGGTATAGTTGAATTAATCATTGACGTGGGTTTGTGTGAAACAGGCGATTAGTGCATATGGTGTAGCAGAAGTAGTGGCTTCGGGCGACCCTGGATTTGAAAAGGGGGACTTGGTTTTTGGGATCATACATTGGGCACAATACAGCCTCGTAAAAGCTGGAGAAAGCACTCTAAGAAAGTTGGATACTTTGGGATTTCCATTCACATACCATCTCGGAGTTTTAGGTCAAAATCAGTTACCAATTGTAATGCAGTTCTTTTCTTTCTTTCTTTCCTAATATTTATATATATGTTCTTGTTTTATTTGTATTGTATTCAGGGTTGAGTGGGCTCACAGCGTACGTGGGATTGTTTGAAGTAGCGAAGATTAAGGAAGGAGAGAAAGTGTTTGTGTCTGCAGCTTCTGGGTCTGTTGGGAATTTAGTTGGACAGTTTGCAAAGCTCCACGGTTGCCACGTTGTCGGCTCTGCTGGTTCGGATCACAAGGTTTTTAATACCATTTTATTTAGTAAAATATATGTTAATTATCGTTGAACTAAAATGCAAGTCTTTAATACTTAACTATTCAACGATCACCGTTATATTTTTTATATATTAAAGTACGACTGGAGAATTGAGATTTTTTTTTAAAAATAAATAAGTAAATATTAAAATTTTAATGGTGGTTAGTGTTAAAGATTCACCCTACAAAAATTCATAATAGACGTAGATTACCCTAAAATATATATTTTTTTTTTCTGGGAAGGTTTTGAGTCAACGACTCCCAAACACTAATTTAAACATAAATATTTCACAAATTTATTGGTTATATTTATACTCATTTTATCCAAGTCAAATTTAAGCAATAATGGAGGGTAATTGGTATTTATGGTAGATTTGAAGGTTAAGTTACCAGTTTTGTTCTCCAACATTTAAGAATGTATTTAATAGATTGTCGAACTTTGAAGGGTATAATTAGTTCCTAAACTTTTAACGAAGGAATGAGTTCAAACCATGATGGTCACTTACATTAGGAATTAAGTAATCCTATGTGTTTTTCGATACCAAATGTTATAAGATCAGTTGTCCGTGAAATTAGTCGAGGTACGCGTTAAGCTGCCTCGAATAAAAATTTGTATCAATAAATCCCTAAAATTTAAAAGTGTTTACTAGATCCTAATCATTACATTTTTTTTTAAGTTAATAAATTTATTAGATCAAAATTAACACTTTTAAGAGTTCTATTATCTTCTCATGTTTTTATTTTTAAATGTTATTTATATTTAACAATACTTAAATACTAACGGATCCTCGTAGGTGACATTTCTGAAAGAGAAGCTCGGATTTGACGAAGCGTTCAACTACAAGCAAGAAAAGGACTTGAAATCCACCTTAGCCAAGTGGGAAATCTCACAATTCCATTGATTTTATAAATTTTGGACACCAAAAATACACATTAAAAGAGATTGAAGAAAATCTACTAATTAAGTATACTAACTAACGATAAGTTATTTATTTGGAAATTAATGAATTTTCCGGCAGGTATTTTCCGGAGGGGATTGATGTTTATTTCGACAACGTGGGCGGCGAGATGCTAGCCGCCGCCGTGGCGAACATGAACCCGTTCGGCAGAGTGGCGGCGTGCGGCGTTATTTCAGAGTACACAAAATCAAAAACCGGACCACCGGCCGGCCCAGGCCCAAACATGGTGGACATTGTGTATAAAAGGATCGCAATCCAAGGCTTTTTAGTCATCGATTATTTTAATGTTTTTCCAGATTTCATATCCAAAACCGCCGGCTACCTCCGCTCCGGCGAGATTCAGGCGGCGGAAGATGTATCGGTGGGTGTCCACAGCATTCCCTCTGCTTTCGTCGGCCTCTTCCATGGCCATAACCTTGGGAAGAAGATAGTCAAGCTTGCTTAATTAATAATTAAGCAATATTATTATTATTATTATTATGTTGTTTCCTTTTCGTGGAATAATTTTAAACTTACACAACTCATTTTGAATAAATTCTACTTTATAACACGGTTACTC

mRNA sequence

ATGAACATCCACTCCTCTTCCCACAACGTTTCATCTGCTACACCTTCTATCGTTCCCGGACAGGCGATTAGTGCATATGGTGTAGCAGAAGTAGTGGCTTCGGGCGACCCTGGATTTGAAAAGGGGGACTTGGTTTTTGGGATCATACATTGGGCACAATACAGCCTCGTAAAAGCTGGAGAAAGCACTCTAAGAAAGTTGGATACTTTGGGATTTCCATTCACATACCATCTCGGAGTTTTAGGGTTGAGTGGGCTCACAGCGTACGTGGGATTGTTTGAAGTAGCGAAGATTAAGGAAGGAGAGAAAGTGTTTGTGTCTGCAGCTTCTGGGTCTGTTGGGAATTTAGTTGGACAGTTTGCAAAGCTCCACGGTTGCCACGTTGTCGGCTCTGCTGGTTCGGATCACAAGGTGACATTTCTGAAAGAGAAGCTCGGATTTGACGAAGCGTTCAACTACAAGCAAGAAAAGGACTTGAAATCCACCTTAGCCAAGTATTTTCCGGAGGGGATTGATGTTTATTTCGACAACGTGGGCGGCGAGATGCTAGCCGCCGCCGTGGCGAACATGAACCCGTTCGGCAGAGTGGCGGCGTGCGGCGTTATTTCAGAGTACACAAAATCAAAAACCGGACCACCGGCCGGCCCAGGCCCAAACATGGTGGACATTGTGTATAAAAGGATCGCAATCCAAGGCTTTTTAGTCATCGATTATTTTAATGTTTTTCCAGATTTCATATCCAAAACCGCCGGCTACCTCCGCTCCGGCGAGATTCAGGCGGCGGAAGATGTATCGGTGGGTGTCCACAGCATTCCCTCTGCTTTCGTCGGCCTCTTCCATGGCCATAACCTTGGGAAGAAGATAGTCAAGCTTGCTTAA

Coding sequence (CDS)

ATGAACATCCACTCCTCTTCCCACAACGTTTCATCTGCTACACCTTCTATCGTTCCCGGACAGGCGATTAGTGCATATGGTGTAGCAGAAGTAGTGGCTTCGGGCGACCCTGGATTTGAAAAGGGGGACTTGGTTTTTGGGATCATACATTGGGCACAATACAGCCTCGTAAAAGCTGGAGAAAGCACTCTAAGAAAGTTGGATACTTTGGGATTTCCATTCACATACCATCTCGGAGTTTTAGGGTTGAGTGGGCTCACAGCGTACGTGGGATTGTTTGAAGTAGCGAAGATTAAGGAAGGAGAGAAAGTGTTTGTGTCTGCAGCTTCTGGGTCTGTTGGGAATTTAGTTGGACAGTTTGCAAAGCTCCACGGTTGCCACGTTGTCGGCTCTGCTGGTTCGGATCACAAGGTGACATTTCTGAAAGAGAAGCTCGGATTTGACGAAGCGTTCAACTACAAGCAAGAAAAGGACTTGAAATCCACCTTAGCCAAGTATTTTCCGGAGGGGATTGATGTTTATTTCGACAACGTGGGCGGCGAGATGCTAGCCGCCGCCGTGGCGAACATGAACCCGTTCGGCAGAGTGGCGGCGTGCGGCGTTATTTCAGAGTACACAAAATCAAAAACCGGACCACCGGCCGGCCCAGGCCCAAACATGGTGGACATTGTGTATAAAAGGATCGCAATCCAAGGCTTTTTAGTCATCGATTATTTTAATGTTTTTCCAGATTTCATATCCAAAACCGCCGGCTACCTCCGCTCCGGCGAGATTCAGGCGGCGGAAGATGTATCGGTGGGTGTCCACAGCATTCCCTCTGCTTTCGTCGGCCTCTTCCATGGCCATAACCTTGGGAAGAAGATAGTCAAGCTTGCTTAA

Protein sequence

MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA
Homology
BLAST of MC06g_new0117 vs. ExPASy Swiss-Prot
Match: Q9SLN8 (2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 7.7e-78
Identity = 140/281 (49.82%), Postives = 198/281 (70.46%), Query Frame = 0

Query: 12  SATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLDTLG 71
           S   S  PG  I+ YGVA+V+ SGDP F+KGDLV+G+  W +YS++   + TL K+    
Sbjct: 66  SYVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQ-TLFKIHDKD 125

Query: 72  FPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVVGS 131
            P +Y+ G+LG+ G+TAY G  EV   K+GE VFVSAASG+VG LVGQFAK+ GC+VVGS
Sbjct: 126 VPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGS 185

Query: 132 AGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVANMN 191
           AGS  KV  LK K GFDEAFNYK+E+DL + L +YFP+GID+YF+NVGG+ML A + NM 
Sbjct: 186 AGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLVNMK 245

Query: 192 PFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKTAG 251
            +GR+A CG+IS+Y   +T        N+  ++ KRI ++GFLV DY++++P ++     
Sbjct: 246 LYGRIAVCGMISQYNLEQTEGV----HNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIP 305

Query: 252 YLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
            +++G++   EDV+ G+ S P+A VGLF G N+GK++V ++
Sbjct: 306 QIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVS 341

BLAST of MC06g_new0117 vs. ExPASy Swiss-Prot
Match: Q39173 (NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 GN=P2 PE=2 SV=2)

HSP 1 Score: 287.0 bits (733), Expect = 2.5e-76
Identity = 138/283 (48.76%), Postives = 196/283 (69.26%), Query Frame = 0

Query: 11  SSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLDTL 70
           S+   +  PG+ I  YGV+ V+ SG P ++KGDL++GI+ W +YS++        K+   
Sbjct: 64  SALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMAHMHFKIQHT 123

Query: 71  GFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVVG 130
             P +Y+ G+LG+ G+TAY G +EV   K+GE V+VSAASG+VG LVGQFAK+ GC+VVG
Sbjct: 124 DVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCYVVG 183

Query: 131 SAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVANM 190
           SAGS  KV  LK K GFD+AFNYK+E DL + L + FP+GID+YF+NVGG+ML A + NM
Sbjct: 184 SAGSKEKVDLLKTKFGFDDAFNYKEESDLSAALKRCFPKGIDMYFENVGGKMLDAVLLNM 243

Query: 191 NPFGRVAACGVISEYT-KSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKT 250
           NP GR+A CG+IS+Y  +++ G       N+ +I+YKRI IQGF+V D+++ +P F+   
Sbjct: 244 NPHGRIAVCGMISQYNLENQEGV-----HNLSNIIYKRIRIQGFVVADFYDKYPKFLELV 303

Query: 251 AGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
              ++ G+I   EDV+ G+   P A VGLFHG N+GK++V +A
Sbjct: 304 LPRIKEGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVIA 341

BLAST of MC06g_new0117 vs. ExPASy Swiss-Prot
Match: Q39172 (NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=3702 GN=AER PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.6e-75
Identity = 136/285 (47.72%), Postives = 194/285 (68.07%), Query Frame = 0

Query: 9   NVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLD 68
           + ++   +  PGQ I  YGV+ ++ SG P ++KGDL++GI+ W +YS++        K+ 
Sbjct: 64  STAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKIQ 123

Query: 69  TLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHV 128
               P +Y+ G+LG+ G+TAY G +EV   KEGE V+VSAASG+VG LVGQ AK+ GC+V
Sbjct: 124 HTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCYV 183

Query: 129 VGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVA 188
           VGSAGS  KV  LK K GFD+AFNYK+E DL + L + FP GID+YF+NVGG+ML A + 
Sbjct: 184 VGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKMLDAVLV 243

Query: 189 NMNPFGRVAACGVISEYT-KSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFIS 248
           NMN  GR+A CG+IS+Y  +++ G       N+ +I+YKRI IQGF+V D+++ +  F+ 
Sbjct: 244 NMNMHGRIAVCGMISQYNLENQEGV-----HNLSNIIYKRIRIQGFVVSDFYDKYSKFLE 303

Query: 249 KTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
               ++R G+I   EDV+ G+   P A VGLFHG N+GK++V +A
Sbjct: 304 FVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVA 343

BLAST of MC06g_new0117 vs. ExPASy Swiss-Prot
Match: Q6WAU0 ((+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.8e-72
Identity = 129/278 (46.40%), Postives = 196/278 (70.50%), Query Frame = 0

Query: 16  SIVPGQAISAYGVAEVVASGDPGFEKGDLVFGI-IHWAQYSLVKAGESTLRKLDTLGFPF 75
           SI+PG  I +YGV++V+ S  P +EKG+L++G    W +Y+L++     L K+     P 
Sbjct: 68  SILPGSTIVSYGVSKVLDSTHPSYEKGELIWGSQAGWEEYTLIQ-NPYNLFKIQDKDVPL 127

Query: 76  TYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVVGSAGS 135
           +Y++G+LG+ G+TAY G FE+   K+GE VFV+AA+GSVG LVGQFAK+ GC+VVGSAGS
Sbjct: 128 SYYVGILGMPGMTAYAGFFEICSPKKGETVFVTAAAGSVGQLVGQFAKMFGCYVVGSAGS 187

Query: 136 DHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVANMNPFG 195
             KV  LK K GFD+AFNYK+E D  + L ++FPEGID+YFDNVGG+ML A + NM   G
Sbjct: 188 KEKVDLLKNKFGFDDAFNYKEESDYDTALKRHFPEGIDIYFDNVGGKMLEAVINNMRVHG 247

Query: 196 RVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKTAGYLR 255
           R+A CG++S+Y+  +   P G   N++ ++ K+I +QGF+V+DY++++P F+      ++
Sbjct: 248 RIAVCGMVSQYSLKQ---PEGV-HNLLKLIPKQIRMQGFVVVDYYHLYPKFLEMVLPRIK 307

Query: 256 SGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
            G++   ED+S G+ S PSA +G++ G N+G ++V ++
Sbjct: 308 EGKVTYVEDISEGLESAPSALLGVYVGRNVGNQVVAVS 340

BLAST of MC06g_new0117 vs. ExPASy Swiss-Prot
Match: O34812 (Putative NADP-dependent oxidoreductase YfmJ OS=Bacillus subtilis (strain 168) OX=224308 GN=yfmJ PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 4.7e-59
Identity = 117/272 (43.01%), Postives = 174/272 (63.97%), Query Frame = 0

Query: 21  QAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLDTLGFPFTYHLGV 80
           +A+S   +AEVV+ G+   +KGD+V G + W ++S V   ES LRK+DT   P + +LG+
Sbjct: 69  KALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAV--SESALRKIDTSLAPASAYLGI 128

Query: 81  LGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVVGSAGSDHKVTF 140
           LG++GLTAY GL ++ + KEGE V VS A+G+VG+ VGQ AK+ G  VVG AGSD K+ +
Sbjct: 129 LGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDY 188

Query: 141 LKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVANMNPFGRVAACG 200
           LK++L FDEA NYK   D++  L    P+G+DVYFDNVGG +  A +  +N F R+  CG
Sbjct: 189 LKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCG 248

Query: 201 VISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKTAGYLRSGEIQA 260
            IS Y         GP      ++  +  +QGF+V DY + F +   + A +L++G++  
Sbjct: 249 AISSYNAESEADDMGPRVQS-KLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHY 308

Query: 261 AEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
            E ++ G  +IP AF+GLF G N GK+++K++
Sbjct: 309 EETITEGFENIPDAFLGLFKGENKGKQLIKVS 336

BLAST of MC06g_new0117 vs. NCBI nr
Match: XP_022145088.1 (2-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia])

HSP 1 Score: 577 bits (1488), Expect = 1.05e-206
Identity = 291/292 (99.66%), Postives = 291/292 (99.66%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG
Sbjct: 62  MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 121

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
           ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF
Sbjct: 122 ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 181

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFD VGG
Sbjct: 182 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFD-VGG 241

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN
Sbjct: 242 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 301

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA
Sbjct: 302 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 352

BLAST of MC06g_new0117 vs. NCBI nr
Match: XP_038878370.1 (NADPH-dependent oxidoreductase 2-alkenal reductase-like [Benincasa hispida])

HSP 1 Score: 431 bits (1109), Expect = 4.92e-149
Identity = 214/292 (73.29%), Postives = 240/292 (82.19%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M  HS SH VSS TP I+PGQ I  +GVAEV+ S D  F+KGD V GIIHWAQYS+VKAG
Sbjct: 61  MKTHSPSHTVSSVTPYIIPGQVIDVHGVAEVMDSDDSEFQKGDFVKGIIHWAQYSVVKAG 120

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
           ES L KLDT+ FP TY+LG+LG SGL+AY GLF+V  +KEG+ VFVSAASGSVGNLVGQF
Sbjct: 121 ESALMKLDTMEFPLTYYLGILGTSGLSAYAGLFDVGNVKEGDNVFVSAASGSVGNLVGQF 180

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKL GC+VVGSAGS+ KV  LKEKLGFDEAFNY Q+KDLKSTL  YFP GID+YFDNVG 
Sbjct: 181 AKLQGCYVVGSAGSNQKVALLKEKLGFDEAFNYNQQKDLKSTLPMYFPNGIDIYFDNVGD 240

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EML A +ANMNPFGRV  CGVISEYTKS  G   G   NM+DIVYKRI IQGFLV DY N
Sbjct: 241 EMLEAVIANMNPFGRVVVCGVISEYTKSTKGK--GGTINMMDIVYKRIKIQGFLVSDYLN 300

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           VF DF SKTAGYLRSGE++A ED+S G+H+IPSAF+GLFHG+N+GKKIVKLA
Sbjct: 301 VFQDFKSKTAGYLRSGEMKAVEDISEGLHNIPSAFIGLFHGNNIGKKIVKLA 350

BLAST of MC06g_new0117 vs. NCBI nr
Match: XP_011656292.1 (2-alkenal reductase (NADP(+)-dependent) [Cucumis sativus] >KAE8653091.1 hypothetical protein Csa_019796 [Cucumis sativus])

HSP 1 Score: 415 bits (1066), Expect = 1.42e-142
Identity = 206/292 (70.55%), Postives = 239/292 (81.85%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M   SSSH  SS    ++P Q I A GV EV+ASG+P F++GD+V G+I+WA+YS+VKA 
Sbjct: 58  MKSFSSSHKTSSFAAPVIPFQPIDALGVGEVLASGNPEFKEGDIVIGLINWAEYSVVKAQ 117

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
            + LRKLDTLGFP T+HLGVLG SGL+AY GLFEVAKIKEGE+VFVSAASGSVG+LVGQ 
Sbjct: 118 TNLLRKLDTLGFPLTHHLGVLGFSGLSAYAGLFEVAKIKEGERVFVSAASGSVGSLVGQL 177

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKLHGC+VVG AGSD KVT LKEKLGFD+AFNYKQEKDL +TL KYFP+GIDVYFDNVGG
Sbjct: 178 AKLHGCYVVGCAGSDQKVTLLKEKLGFDDAFNYKQEKDLTTTLEKYFPDGIDVYFDNVGG 237

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EML AA+ANM PFGRVA CGVISEYT SK   P     NMVD+VYKRI +QGFL  D+ +
Sbjct: 238 EMLEAAIANMKPFGRVAVCGVISEYTNSKKAVP-----NMVDLVYKRINVQGFLAGDFLD 297

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           VFP+F+SK + YL SGEI+  ED+SVGV +IPSAF+GLF G N+GKKIVK A
Sbjct: 298 VFPNFVSKVSQYLHSGEIEPLEDISVGVENIPSAFIGLFKGDNIGKKIVKFA 344

BLAST of MC06g_new0117 vs. NCBI nr
Match: XP_008444008.1 (PREDICTED: 2-alkenal reductase (NADP(+)-dependent)-like [Cucumis melo])

HSP 1 Score: 410 bits (1053), Expect = 1.35e-140
Identity = 201/292 (68.84%), Postives = 235/292 (80.48%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M   SS+H  S     I+P Q I A GV EV+ASG+P F++GD+V GI HWA+YS+VKA 
Sbjct: 58  MKSFSSTHKTSDFATPIIPSQPIDALGVGEVLASGNPEFKEGDIVIGITHWAEYSVVKAH 117

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
            + LRKL+TLGFP T+HLG+LG SGLTAY GLFEVAKIKEGE VFVSAASGSVG+LVGQF
Sbjct: 118 TNLLRKLETLGFPLTHHLGILGFSGLTAYAGLFEVAKIKEGETVFVSAASGSVGSLVGQF 177

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKLHGC+VVG AGSD KVT LKEK GFD AFNYKQEKDL +TL KYFP+GIDVYFDNVGG
Sbjct: 178 AKLHGCYVVGCAGSDQKVTLLKEKFGFDNAFNYKQEKDLTATLEKYFPDGIDVYFDNVGG 237

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EML AA+ANM PFGRVA CGVISEYT SK   P     NM+D+VYKRI ++GFL  D+ +
Sbjct: 238 EMLEAAIANMKPFGRVAVCGVISEYTNSKKAVP-----NMIDLVYKRIKVEGFLAGDFLD 297

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           +FP+F+SK + YL SG+I+  ED+SVGV +IPSAF+GLF G N+GKKIVK A
Sbjct: 298 MFPNFVSKVSQYLHSGKIEPLEDISVGVENIPSAFIGLFKGDNIGKKIVKFA 344

BLAST of MC06g_new0117 vs. NCBI nr
Match: XP_022145101.1 (2-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia])

HSP 1 Score: 409 bits (1052), Expect = 1.98e-140
Identity = 206/293 (70.31%), Postives = 238/293 (81.23%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M  H+SSH  SS    I PGQ I  +G AEVVASG+P F +GDLV G+I+WA+Y++VKA 
Sbjct: 58  MKSHNSSHKTSSFATPISPGQPIDGFGAAEVVASGNPDFRRGDLVVGLINWAEYTVVKAQ 117

Query: 61  ESTLRKLDT-LGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQ 120
              LRKLDT L FP T+HLG+ GLSGLTA+VGLF VAK K+GEKVFVSAASGSVGNLVGQ
Sbjct: 118 NRLLRKLDTALEFPITHHLGIFGLSGLTAHVGLFVVAKAKKGEKVFVSAASGSVGNLVGQ 177

Query: 121 FAKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVG 180
           FAKLHGC+V+G AGSD KVT LKEKLGFD+AFNYKQEKDL STL KYFP+GIDVYFDNVG
Sbjct: 178 FAKLHGCYVIGCAGSDQKVTVLKEKLGFDDAFNYKQEKDLTSTLEKYFPDGIDVYFDNVG 237

Query: 181 GEMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYF 240
           G ML AAVAN+N FGRVA CGVISEYT SK        PNM+D+VYKRI I+GFL  D+F
Sbjct: 238 GVMLEAAVANLNTFGRVALCGVISEYTNSKKA-----APNMLDLVYKRIKIEGFLAGDHF 297

Query: 241 NVFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           +V+ DFI+K + Y+RSG+I+  EDVS+G+HSIPSAFVGLF G N+GKKIVK A
Sbjct: 298 DVYSDFIAKASDYIRSGKIETLEDVSMGIHSIPSAFVGLFKGDNIGKKIVKFA 345

BLAST of MC06g_new0117 vs. ExPASy TrEMBL
Match: A0A6J1CV06 (2-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=LOC111014596 PE=4 SV=1)

HSP 1 Score: 577 bits (1488), Expect = 5.11e-207
Identity = 291/292 (99.66%), Postives = 291/292 (99.66%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG
Sbjct: 62  MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 121

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
           ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF
Sbjct: 122 ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 181

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFD VGG
Sbjct: 182 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFD-VGG 241

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN
Sbjct: 242 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 301

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA
Sbjct: 302 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 352

BLAST of MC06g_new0117 vs. ExPASy TrEMBL
Match: A0A1S3B9C7 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucumis melo OX=3656 GN=LOC103487456 PE=4 SV=1)

HSP 1 Score: 410 bits (1053), Expect = 6.53e-141
Identity = 201/292 (68.84%), Postives = 235/292 (80.48%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M   SS+H  S     I+P Q I A GV EV+ASG+P F++GD+V GI HWA+YS+VKA 
Sbjct: 58  MKSFSSTHKTSDFATPIIPSQPIDALGVGEVLASGNPEFKEGDIVIGITHWAEYSVVKAH 117

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
            + LRKL+TLGFP T+HLG+LG SGLTAY GLFEVAKIKEGE VFVSAASGSVG+LVGQF
Sbjct: 118 TNLLRKLETLGFPLTHHLGILGFSGLTAYAGLFEVAKIKEGETVFVSAASGSVGSLVGQF 177

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKLHGC+VVG AGSD KVT LKEK GFD AFNYKQEKDL +TL KYFP+GIDVYFDNVGG
Sbjct: 178 AKLHGCYVVGCAGSDQKVTLLKEKFGFDNAFNYKQEKDLTATLEKYFPDGIDVYFDNVGG 237

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EML AA+ANM PFGRVA CGVISEYT SK   P     NM+D+VYKRI ++GFL  D+ +
Sbjct: 238 EMLEAAIANMKPFGRVAVCGVISEYTNSKKAVP-----NMIDLVYKRIKVEGFLAGDFLD 297

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           +FP+F+SK + YL SG+I+  ED+SVGV +IPSAF+GLF G N+GKKIVK A
Sbjct: 298 MFPNFVSKVSQYLHSGKIEPLEDISVGVENIPSAFIGLFKGDNIGKKIVKFA 344

BLAST of MC06g_new0117 vs. ExPASy TrEMBL
Match: A0A6J1CVC7 (2-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=LOC111014607 PE=4 SV=1)

HSP 1 Score: 409 bits (1052), Expect = 9.59e-141
Identity = 206/293 (70.31%), Postives = 238/293 (81.23%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M  H+SSH  SS    I PGQ I  +G AEVVASG+P F +GDLV G+I+WA+Y++VKA 
Sbjct: 58  MKSHNSSHKTSSFATPISPGQPIDGFGAAEVVASGNPDFRRGDLVVGLINWAEYTVVKAQ 117

Query: 61  ESTLRKLDT-LGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQ 120
              LRKLDT L FP T+HLG+ GLSGLTA+VGLF VAK K+GEKVFVSAASGSVGNLVGQ
Sbjct: 118 NRLLRKLDTALEFPITHHLGIFGLSGLTAHVGLFVVAKAKKGEKVFVSAASGSVGNLVGQ 177

Query: 121 FAKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVG 180
           FAKLHGC+V+G AGSD KVT LKEKLGFD+AFNYKQEKDL STL KYFP+GIDVYFDNVG
Sbjct: 178 FAKLHGCYVIGCAGSDQKVTVLKEKLGFDDAFNYKQEKDLTSTLEKYFPDGIDVYFDNVG 237

Query: 181 GEMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYF 240
           G ML AAVAN+N FGRVA CGVISEYT SK        PNM+D+VYKRI I+GFL  D+F
Sbjct: 238 GVMLEAAVANLNTFGRVALCGVISEYTNSKKA-----APNMLDLVYKRIKIEGFLAGDHF 297

Query: 241 NVFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           +V+ DFI+K + Y+RSG+I+  EDVS+G+HSIPSAFVGLF G N+GKKIVK A
Sbjct: 298 DVYSDFIAKASDYIRSGKIETLEDVSMGIHSIPSAFVGLFKGDNIGKKIVKFA 345

BLAST of MC06g_new0117 vs. ExPASy TrEMBL
Match: A0A6J1I7M4 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita maxima OX=3661 GN=LOC111470739 PE=4 SV=1)

HSP 1 Score: 407 bits (1045), Expect = 1.07e-139
Identity = 201/292 (68.84%), Postives = 236/292 (80.82%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M  HSSS   SS   SI P Q I AYGV EV+ASG+  F+KGDLV G++HWA+YS+VKA 
Sbjct: 58  MKTHSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLVHWAEYSIVKAH 117

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
            + LRK+D++GFP T HLG+LGLSGLTAY GLFEVAK+KEGE VFVSAASGSVG+L GQ 
Sbjct: 118 TNMLRKIDSMGFPLTNHLGILGLSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQL 177

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKLHGC+VVGSAGSD KV  LKEKLGFDEAFNYKQEKDL S+L +YFP+GIDVYFDNVGG
Sbjct: 178 AKLHGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSSLERYFPDGIDVYFDNVGG 237

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EML AAVAN+ PFGRVA CGVISEYT SK        P M+D+VYKRI ++GFL  D+ N
Sbjct: 238 EMLEAAVANLKPFGRVALCGVISEYTSSKKA-----APKMLDLVYKRIKVEGFLAGDFLN 297

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           V+ DFI+  + YL SG+I++ EDV++GV +IPSAF+GLF G N+GKKIVKLA
Sbjct: 298 VYSDFIAMASQYLGSGKIESLEDVTIGVDTIPSAFIGLFKGDNIGKKIVKLA 344

BLAST of MC06g_new0117 vs. ExPASy TrEMBL
Match: A0A6J1F1I6 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita moschata OX=3662 GN=LOC111441259 PE=4 SV=1)

HSP 1 Score: 396 bits (1018), Expect = 1.36e-135
Identity = 198/292 (67.81%), Postives = 232/292 (79.45%), Query Frame = 0

Query: 1   MNIHSSSHNVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAG 60
           M  HSSS   SS   SI P Q I AYGV EV+ASG+  F+KGDLV G++HWA+YS+VKA 
Sbjct: 58  MKSHSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQ 117

Query: 61  ESTLRKLDTLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQF 120
            S LR++D++G P T HLG+LG SGLTAY GLFEVAK+KEGE VFVSAASGSVG+L GQ 
Sbjct: 118 TSLLRQIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQL 177

Query: 121 AKLHGCHVVGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGG 180
           AKLHGC+VVGSAGSD KV  LKEKLGFDEAFNYKQE+DL STL +YFP+GIDVYFDNVGG
Sbjct: 178 AKLHGCYVVGSAGSDEKVGLLKEKLGFDEAFNYKQERDLTSTLERYFPDGIDVYFDNVGG 237

Query: 181 EMLAAAVANMNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFN 240
           EML AAVAN+  FGRVA CGVISEYT SK        P M+D+VYKRI ++GFL  D+ N
Sbjct: 238 EMLEAAVANLKAFGRVALCGVISEYTSSKKA-----APKMLDLVYKRIKVEGFLAGDFLN 297

Query: 241 VFPDFISKTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 292
           V+ DFI+  + YL S +I++ EDVS+GV +IPSAF+GLF G N+GKKIVKLA
Sbjct: 298 VYSDFIAMVSQYLGSRKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKIVKLA 344

BLAST of MC06g_new0117 vs. TAIR 10
Match: AT5G16990.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 287.0 bits (733), Expect = 1.7e-77
Identity = 138/283 (48.76%), Postives = 196/283 (69.26%), Query Frame = 0

Query: 11  SSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLDTL 70
           S+   +  PG+ I  YGV+ V+ SG P ++KGDL++GI+ W +YS++        K+   
Sbjct: 64  SALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMAHMHFKIQHT 123

Query: 71  GFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVVG 130
             P +Y+ G+LG+ G+TAY G +EV   K+GE V+VSAASG+VG LVGQFAK+ GC+VVG
Sbjct: 124 DVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCYVVG 183

Query: 131 SAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVANM 190
           SAGS  KV  LK K GFD+AFNYK+E DL + L + FP+GID+YF+NVGG+ML A + NM
Sbjct: 184 SAGSKEKVDLLKTKFGFDDAFNYKEESDLSAALKRCFPKGIDMYFENVGGKMLDAVLLNM 243

Query: 191 NPFGRVAACGVISEYT-KSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKT 250
           NP GR+A CG+IS+Y  +++ G       N+ +I+YKRI IQGF+V D+++ +P F+   
Sbjct: 244 NPHGRIAVCGMISQYNLENQEGV-----HNLSNIIYKRIRIQGFVVADFYDKYPKFLELV 303

Query: 251 AGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
              ++ G+I   EDV+ G+   P A VGLFHG N+GK++V +A
Sbjct: 304 LPRIKEGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVIA 341

BLAST of MC06g_new0117 vs. TAIR 10
Match: AT5G16960.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 284.6 bits (727), Expect = 8.7e-77
Identity = 138/274 (50.36%), Postives = 193/274 (70.44%), Query Frame = 0

Query: 16  SIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLDTLGFPFT 75
           S  PG+ IS +GV++V+ SG   +E+GDL++G + W +YS++    +   K+    FP +
Sbjct: 72  SFTPGKPISGFGVSKVIDSGHSDYEEGDLIWGAVGWEEYSVITPIPNLHFKIHHTNFPLS 131

Query: 76  YHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVVGSAGSD 135
           Y+ G+LG+ G+TAYVG +E+   K+G+ VFVSAASG+VG LVGQFAKL GC+VVGSAGS 
Sbjct: 132 YYTGLLGMPGMTAYVGFYEICTPKKGDTVFVSAASGAVGQLVGQFAKLMGCYVVGSAGSK 191

Query: 136 HKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVANMNPFGR 195
            KV  LK K GFD+AFNYK+E +L   L + FPEGID+YF+NVGG+ML A + NM P GR
Sbjct: 192 EKVDLLKNKFGFDDAFNYKEEHNLIGALKRCFPEGIDIYFENVGGKMLDAVILNMRPHGR 251

Query: 196 VAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKTAGYLRS 255
           +AACG+IS+Y  +   P    G ++  I YKRI I+GF   DYF+ + +F+     Y++ 
Sbjct: 252 IAACGMISQY--NLKNPEGIYGLSL--ITYKRIRIEGFNCFDYFHKYSEFLEFVVPYIKE 311

Query: 256 GEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIV 290
           G+I+  EDV+ G+ S P+A VGLFHG N+GK++V
Sbjct: 312 GKIKYVEDVADGLESAPAALVGLFHGKNVGKQLV 341

BLAST of MC06g_new0117 vs. TAIR 10
Match: AT5G17000.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.5e-76
Identity = 137/285 (48.07%), Postives = 194/285 (68.07%), Query Frame = 0

Query: 9   NVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLD 68
           + ++   +  PG+ I  YGV+ V+ SG P ++KGDL++GI+ W +YS++        K+ 
Sbjct: 64  STAALAQAYAPGKPIFGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMTHMHFKIQ 123

Query: 69  TLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHV 128
               P +Y+ G+LG+ G+TAY G +EV   KEGE V+VSAASG+VG LVGQFAK+ GC+V
Sbjct: 124 HTDIPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQFAKMMGCYV 183

Query: 129 VGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVA 188
           VGSAGS  KV  LK K GFD+AFNYK+E DL + L + FP GID+YF+NVGG+ML A + 
Sbjct: 184 VGSAGSTEKVDLLKTKFGFDDAFNYKEEPDLSAALKRCFPTGIDIYFENVGGKMLDAVLL 243

Query: 189 NMNPFGRVAACGVISEYT-KSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFIS 248
           NMNP GR+A CG+IS+Y  +++ G       N+ +I+YKRI IQGF V D++  +  F+ 
Sbjct: 244 NMNPHGRIAVCGMISQYNLENQEGV-----HNLSNIIYKRIRIQGFAVFDFYEKYSKFLD 303

Query: 249 KTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
               +++ G+I   EDV+ G+   P A VGLFHG N+GK++V +A
Sbjct: 304 FVLPHIKEGKITYVEDVADGLEKGPEALVGLFHGKNVGKQVVVIA 343

BLAST of MC06g_new0117 vs. TAIR 10
Match: AT1G65560.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 283.5 bits (724), Expect = 1.9e-76
Identity = 140/283 (49.47%), Postives = 192/283 (67.84%), Query Frame = 0

Query: 12  SATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGES-TLRKLD-T 71
           S  P  VPGQ I  +G+A V+ S D  ++ GD+V GII W +YSL+++ ++  LR +   
Sbjct: 69  SYLPPFVPGQRIEGFGLARVIDSDDTNYKPGDIVSGIIGWEEYSLLRSSDNLQLRNIQLD 128

Query: 72  LGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHVV 131
              P +YHLG+LG++G TAY G  E+   K+G+ VFVSAA G+VG LVGQ AKLHGC+VV
Sbjct: 129 DDIPLSYHLGLLGMAGFTAYAGFNEICCPKKGDSVFVSAACGAVGQLVGQLAKLHGCYVV 188

Query: 132 GSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVAN 191
           GSAGS  KV  LK +LG+DEAFNYK+E DL + L +YFPEGID+YFDNVGG ML AA+ N
Sbjct: 189 GSAGSKQKVEILKNELGYDEAFNYKEEADLDTALKRYFPEGIDIYFDNVGGSMLDAALLN 248

Query: 192 MNPFGRVAACGVISEYTKSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFISKT 251
           M   GR+A CG++S  + S +        N+   +YKR+ ++GFL  DY ++FP F+   
Sbjct: 249 MKVRGRIALCGMVSLQSLSTSSQGI---KNLYSAIYKRLRLEGFLQSDYLHIFPQFLENV 308

Query: 252 AGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
             Y + G+I   ED+S G+   P+A VGLF G N+GK++V++A
Sbjct: 309 KRYYKEGKIVYVEDISEGLDLAPAALVGLFSGKNIGKQVVRVA 348

BLAST of MC06g_new0117 vs. TAIR 10
Match: AT5G16970.1 (alkenal reductase )

HSP 1 Score: 283.1 bits (723), Expect = 2.5e-76
Identity = 136/285 (47.72%), Postives = 194/285 (68.07%), Query Frame = 0

Query: 9   NVSSATPSIVPGQAISAYGVAEVVASGDPGFEKGDLVFGIIHWAQYSLVKAGESTLRKLD 68
           + ++   +  PGQ I  YGV+ ++ SG P ++KGDL++GI+ W +YS++        K+ 
Sbjct: 64  STAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKIQ 123

Query: 69  TLGFPFTYHLGVLGLSGLTAYVGLFEVAKIKEGEKVFVSAASGSVGNLVGQFAKLHGCHV 128
               P +Y+ G+LG+ G+TAY G +EV   KEGE V+VSAASG+VG LVGQ AK+ GC+V
Sbjct: 124 HTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCYV 183

Query: 129 VGSAGSDHKVTFLKEKLGFDEAFNYKQEKDLKSTLAKYFPEGIDVYFDNVGGEMLAAAVA 188
           VGSAGS  KV  LK K GFD+AFNYK+E DL + L + FP GID+YF+NVGG+ML A + 
Sbjct: 184 VGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKMLDAVLV 243

Query: 189 NMNPFGRVAACGVISEYT-KSKTGPPAGPGPNMVDIVYKRIAIQGFLVIDYFNVFPDFIS 248
           NMN  GR+A CG+IS+Y  +++ G       N+ +I+YKRI IQGF+V D+++ +  F+ 
Sbjct: 244 NMNMHGRIAVCGMISQYNLENQEGV-----HNLSNIIYKRIRIQGFVVSDFYDKYSKFLE 303

Query: 249 KTAGYLRSGEIQAAEDVSVGVHSIPSAFVGLFHGHNLGKKIVKLA 293
               ++R G+I   EDV+ G+   P A VGLFHG N+GK++V +A
Sbjct: 304 FVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVA 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SLN87.7e-7849.822-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1... [more]
Q391732.5e-7648.76NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q391723.6e-7547.72NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=37... [more]
Q6WAU02.8e-7246.40(+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1[more]
O348124.7e-5943.01Putative NADP-dependent oxidoreductase YfmJ OS=Bacillus subtilis (strain 168) OX... [more]
Match NameE-valueIdentityDescription
XP_022145088.11.05e-20699.662-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia][more]
XP_038878370.14.92e-14973.29NADPH-dependent oxidoreductase 2-alkenal reductase-like [Benincasa hispida][more]
XP_011656292.11.42e-14270.552-alkenal reductase (NADP(+)-dependent) [Cucumis sativus] >KAE8653091.1 hypothet... [more]
XP_008444008.11.35e-14068.84PREDICTED: 2-alkenal reductase (NADP(+)-dependent)-like [Cucumis melo][more]
XP_022145101.11.98e-14070.312-alkenal reductase (NADP(+)-dependent)-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1CV065.11e-20799.662-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A1S3B9C76.53e-14168.842-alkenal reductase (NADP(+)-dependent)-like OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1CVC79.59e-14170.312-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1I7M41.07e-13968.842-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F1I61.36e-13567.812-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G16990.11.7e-7748.76Zinc-binding dehydrogenase family protein [more]
AT5G16960.18.7e-7750.36Zinc-binding dehydrogenase family protein [more]
AT5G17000.11.5e-7648.07Zinc-binding dehydrogenase family protein [more]
AT1G65560.11.9e-7649.47Zinc-binding dehydrogenase family protein [more]
AT5G16970.12.5e-7647.72alkenal reductase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020843Polyketide synthase, enoylreductase domainSMARTSM00829PKS_ER_names_modcoord: 2..289
e-value: 1.4E-5
score: -95.2
IPR041694Oxidoreductase, N-terminal domainPFAMPF16884ADH_N_2coord: 10..60
e-value: 1.4E-7
score: 31.4
IPR013149Alcohol dehydrogenase, C-terminalPFAMPF00107ADH_zinc_Ncoord: 113..246
e-value: 5.7E-23
score: 81.3
NoneNo IPR availableGENE3D3.90.180.10coord: 3..289
e-value: 6.7E-114
score: 382.1
NoneNo IPR availableGENE3D3.40.50.720coord: 80..260
e-value: 6.7E-114
score: 382.1
NoneNo IPR availablePANTHERPTHR43205:SF12OS06G0602900 PROTEINcoord: 1..292
IPR045010Medium-chain dehydrogenase/reductasePANTHERPTHR43205PROSTAGLANDIN REDUCTASEcoord: 1..292
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 77..256
IPR011032GroES-like superfamilySUPERFAMILY50129GroES-likecoord: 15..97

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0117.1MC06g_new0117.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function GO:0016491 oxidoreductase activity