MC06g_new0095 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0095
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
LocationMC06: 3651561 .. 3652769 (-)
RNA-Seq ExpressionMC06g_new0095
SyntenyMC06g_new0095
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAATATTACACAATTTTGTTTTGAAAATGGCTCTCTCTTGCTCTTTTCCAAAAAAGCTCAATAATTACTCTCCTGATCCCCTAGTTTTGTTCTTGCTACTTCCTTGTCTTATGGGGTTTGTACTGAAGACGGCAGAAGCTGGGAGCTTCTACCAGGAACTGGACGTAACATGGGGAGATGGCCGGGGAAAGATAATGGAGAATGGGAATCTCCTTACTCTGTCCCTTGACAGAGCCTCTGGTTCAGGCTTCCAGTCAAAGAAGCAATACCTCTTCGGCAGGTTCGATTTGAAAATCAAACTCGTTCCTGCCAACTCAGCAGGCACAGTCACCGCCTATTATGTAAGTCCACTTTTTTCTTTTCTTTTTCTTTTTTTGGTGTGTTTTAATCAACAAATTAAGTAATTGTTCTAATTATTTATTAGTTGCGCTCGCAAGGGTCGTCATGGGATGAAATAGACTTCGAATTCCTTGGAAACTTGAGTGGGGAACCCTACATTGTACATACGAATGTGTATACACAAGGACAAGGCAACAGGGAGCAGCAATTCTACCTGTGGTTTGATCCAACGGCTGATTTTCACACCTACTCCTTCCTATGGAATCCTGCGCACATAGTGTAAGTATTAATAAACTATCGTCCTCCTTGAAGATCGAATAGTGATGGAATTTTTGTTTGACTATGTGTTTTGGGCTGGGCCGGGTCAGGTTCTACGTGGACGGGAGGCCCATCAGAGAGTTCAGGAACGTGGAATCCATGGGGACGCCGTATCCAAACAAACAGGAGATGAGGCTATACGGCAGCCTATGGAACGCCGACGATTGGGCCACGAGAGGAGGGCTGGTGAAGACGGACTGGACCAAGGCCCCCTTCACGGCTTCCTTCAGAAGCTACAATCCCAATGCGTGCGTGTTGTCCAATGGAGCGTCGTCTTGCCGGAACGCCGCGCCGTGGCTGTCGCAGGAGCTCGATTCCGCCACCCAGAGGATGCTGCGATGGGTTCAGAAGAATTATATGATTTATAATTACTGCGCTGACAAAAGGCGATTCCCTCAAGGCCTTCCTCCGGAGTGCACTCTCCCCACCAAGCCATAGGCGGAAGATTAATTCTGTTAAGATTTAATTTATTTTAAGTCCTGTACCTATTTTATTGCGAGTAATTAAATGAATAAAATAACCGACTTCGCATTGGTTTTTTATTTTTGCC

mRNA sequence

ATGGCTCTCTCTTGCTCTTTTCCAAAAAAGCTCAATAATTACTCTCCTGATCCCCTAGTTTTGTTCTTGCTACTTCCTTGTCTTATGGGGTTTGTACTGAAGACGGCAGAAGCTGGGAGCTTCTACCAGGAACTGGACGTAACATGGGGAGATGGCCGGGGAAAGATAATGGAGAATGGGAATCTCCTTACTCTGTCCCTTGACAGAGCCTCTGGTTCAGGCTTCCAGTCAAAGAAGCAATACCTCTTCGGCAGGTTCGATTTGAAAATCAAACTCGTTCCTGCCAACTCAGCAGGCACAGTCACCGCCTATTATTTGCGCTCGCAAGGGTCGTCATGGGATGAAATAGACTTCGAATTCCTTGGAAACTTGAGTGGGGAACCCTACATTGTACATACGAATGTGTATACACAAGGACAAGGCAACAGGGAGCAGCAATTCTACCTGTGGTTTGATCCAACGGCTGATTTTCACACCTACTCCTTCCTATGGAATCCTGCGCACATAGTGTTCTACGTGGACGGGAGGCCCATCAGAGAGTTCAGGAACGTGGAATCCATGGGGACGCCGTATCCAAACAAACAGGAGATGAGGCTATACGGCAGCCTATGGAACGCCGACGATTGGGCCACGAGAGGAGGGCTGGTGAAGACGGACTGGACCAAGGCCCCCTTCACGGCTTCCTTCAGAAGCTACAATCCCAATGCGTGCGTGTTGTCCAATGGAGCGTCGTCTTGCCGGAACGCCGCGCCGTGGCTGTCGCAGGAGCTCGATTCCGCCACCCAGAGGATGCTGCGATGGGTTCAGAAGAATTATATGATTTATAATTACTGCGCTGACAAAAGGCGATTCCCTCAAGGCCTTCCTCCGGAGTGCACTCTCCCCACCAAGCCATAG

Coding sequence (CDS)

ATGGCTCTCTCTTGCTCTTTTCCAAAAAAGCTCAATAATTACTCTCCTGATCCCCTAGTTTTGTTCTTGCTACTTCCTTGTCTTATGGGGTTTGTACTGAAGACGGCAGAAGCTGGGAGCTTCTACCAGGAACTGGACGTAACATGGGGAGATGGCCGGGGAAAGATAATGGAGAATGGGAATCTCCTTACTCTGTCCCTTGACAGAGCCTCTGGTTCAGGCTTCCAGTCAAAGAAGCAATACCTCTTCGGCAGGTTCGATTTGAAAATCAAACTCGTTCCTGCCAACTCAGCAGGCACAGTCACCGCCTATTATTTGCGCTCGCAAGGGTCGTCATGGGATGAAATAGACTTCGAATTCCTTGGAAACTTGAGTGGGGAACCCTACATTGTACATACGAATGTGTATACACAAGGACAAGGCAACAGGGAGCAGCAATTCTACCTGTGGTTTGATCCAACGGCTGATTTTCACACCTACTCCTTCCTATGGAATCCTGCGCACATAGTGTTCTACGTGGACGGGAGGCCCATCAGAGAGTTCAGGAACGTGGAATCCATGGGGACGCCGTATCCAAACAAACAGGAGATGAGGCTATACGGCAGCCTATGGAACGCCGACGATTGGGCCACGAGAGGAGGGCTGGTGAAGACGGACTGGACCAAGGCCCCCTTCACGGCTTCCTTCAGAAGCTACAATCCCAATGCGTGCGTGTTGTCCAATGGAGCGTCGTCTTGCCGGAACGCCGCGCCGTGGCTGTCGCAGGAGCTCGATTCCGCCACCCAGAGGATGCTGCGATGGGTTCAGAAGAATTATATGATTTATAATTACTGCGCTGACAAAAGGCGATTCCCTCAAGGCCTTCCTCCGGAGTGCACTCTCCCCACCAAGCCATAG

Protein sequence

MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTKP
Homology
BLAST of MC06g_new0095 vs. ExPASy Swiss-Prot
Match: Q38857 (Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=3702 GN=XTH22 PE=1 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 5.7e-113
Identity = 180/281 (64.06%), Postives = 226/281 (80.43%), Query Frame = 0

Query: 19  LVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSK 78
           + +  LLP  +  ++ ++ + +F +++++TWGDGRG+I  NG LLTLSLD++SGSGFQSK
Sbjct: 1   MAITYLLPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSK 60

Query: 79  KQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQ 138
            +YLFG+  +++KLVP NSAGTVT  YL+S G++WDEIDFEFLGN SGEPY +HTNVYTQ
Sbjct: 61  NEYLFGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQ 120

Query: 139 GQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMR 198
           G+G++EQQF LWFDPTA+FHTY+ LWNP  I+F VDG PIREF+N+ES+GT +P  + MR
Sbjct: 121 GKGDKEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMR 180

Query: 199 LYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGASSCRNAA-------P 258
           +Y SLWNADDWATRGGLVKTDW+KAPFTAS+R +   ACV SNG SSC NA+        
Sbjct: 181 MYSSLWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGS 240

Query: 259 WLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPEC 293
           WLSQELDS  Q+ +RWVQ+NYMIYNYC D +RFPQGLP EC
Sbjct: 241 WLSQELDSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MC06g_new0095 vs. ExPASy Swiss-Prot
Match: Q38910 (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana OX=3702 GN=XTH23 PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 5.7e-113
Identity = 184/276 (66.67%), Postives = 225/276 (81.52%), Query Frame = 0

Query: 23  LLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSKKQYL 82
           +++  L  F++ +  A +F +++++TWGDGRG+I  NG+LLTLSLD+ASGSGFQSK +YL
Sbjct: 9   IVVALLASFMICSVSA-NFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYL 68

Query: 83  FGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGN 142
           FG+ D++IKLV  NSAGTVTAYYL+S GS+WDEIDFEFLGNLSG+PY +HTNV+TQG+G+
Sbjct: 69  FGKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGD 128

Query: 143 REQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGS 202
           REQQF LWFDPT+DFHTYS LWNP  I+F VDG PIREF+N+ES GT +P  Q MR+Y S
Sbjct: 129 REQQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSS 188

Query: 203 LWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGASSCRNAA------PWLSQE 262
           LWNA++WATRGGLVKTDW+KAPFTAS+R +N  ACV+ NG SSC N +       WLSQE
Sbjct: 189 LWNAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQE 248

Query: 263 LDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPEC 293
           LDS  Q  +RWVQ NYMIYNYC D +RFPQGLP EC
Sbjct: 249 LDSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MC06g_new0095 vs. ExPASy Swiss-Prot
Match: Q38907 (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana OX=3702 GN=XTH25 PE=2 SV=2)

HSP 1 Score: 403.3 bits (1035), Expect = 2.4e-111
Identity = 179/256 (69.92%), Postives = 212/256 (82.81%), Query Frame = 0

Query: 38  AGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANS 97
           AG+F  E D+TWGDGRGK++ NG LLTLSLDRASGSGFQ+KK+YLFG+ D+++KLVP NS
Sbjct: 27  AGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPGNS 86

Query: 98  AGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADF 157
           AGTVTAYYL+S+G +WDEIDFEFLGNL+G+PY +HTNVYTQG+G+REQQF+LWFDPTADF
Sbjct: 87  AGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQGKGDREQQFHLWFDPTADF 146

Query: 158 HTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVK 217
           HTYS LWNP HIVF VD  P+REF+N++ MG  YP  Q MRLY SLWNAD WATRGGLVK
Sbjct: 147 HTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRLYSSLWNADQWATRGGLVK 206

Query: 218 TDWTKAPFTASFRSYNPNACVLSNGASSCRNAAP-WLSQELDSATQRMLRWVQKNYMIYN 277
           TDW+KAPFTAS+R++  +ACV S G SSC   +P W SQ LD   +  +R VQ+ YMIYN
Sbjct: 207 TDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPRWFSQRLDLTAEDKMRVVQRKYMIYN 266

Query: 278 YCADKRRFPQGLPPEC 293
           YC D +RFPQG P EC
Sbjct: 267 YCTDTKRFPQGFPKEC 282

BLAST of MC06g_new0095 vs. ExPASy Swiss-Prot
Match: Q9ZV40 (Probable xyloglucan endotransglucosylase/hydrolase protein 21 OS=Arabidopsis thaliana OX=3702 GN=XTH21 PE=2 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 9.5e-108
Identity = 177/283 (62.54%), Postives = 220/283 (77.74%), Query Frame = 0

Query: 32  VLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSKKQYLFGRFDLKIK 91
           +L       F Q++D+TWGDGRG I+ NG LL L LD++SGSGFQSK +YL+G+ D++IK
Sbjct: 19  ILLVVHGKDFNQDIDITWGDGRGNILNNGTLLNLGLDQSSGSGFQSKAEYLYGKVDMQIK 78

Query: 92  LVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGNREQQFYLWF 151
           LVP NSAGTVT +YL+SQG +WDEIDFEFLGN+SG+PYIVHTNVYTQG+G+REQQFYLWF
Sbjct: 79  LVPGNSAGTVTTFYLKSQGLTWDEIDFEFLGNVSGDPYIVHTNVYTQGKGDREQQFYLWF 138

Query: 152 DPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGSLWNADDWAT 211
           DPTA FH YS LWNP+HIVFY+DG+PIREF+N+E +G  YP  Q MR+YGSLWNADDWAT
Sbjct: 139 DPTAAFHNYSILWNPSHIVFYIDGKPIREFKNLEVLGVAYPKNQPMRMYGSLWNADDWAT 198

Query: 212 RGGLVKTDWTKAPFTASFRSYN-PNACVLS--NG-------------ASSCRNAAPWLSQ 271
           RGGLVKT+W++ PF ASF +YN  NACV S  NG             +SS  + + W SQ
Sbjct: 199 RGGLVKTNWSQGPFVASFMNYNSENACVWSIVNGTTTTSPCSPGDSTSSSSSSTSEWFSQ 258

Query: 272 E-LDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTK 298
             +DS+++++LRWVQ+ +M+YNYC DK+RF  GLP ECT   K
Sbjct: 259 RGMDSSSKKVLRWVQRKFMVYNYCKDKKRFSNGLPVECTAKNK 301

BLAST of MC06g_new0095 vs. ExPASy Swiss-Prot
Match: Q9FI31 (Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=3702 GN=XTH20 PE=2 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 3.7e-104
Identity = 160/257 (62.26%), Postives = 210/257 (81.71%), Query Frame = 0

Query: 38  AGSFYQELDVTWGDGRGKIMEN-GNLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPAN 97
           AGSF++++ + WGDGRGKI++N GNLL+LSLD+ SGSGFQS +++L+G+ ++++KLVP N
Sbjct: 26  AGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFLYGKVEVQMKLVPGN 85

Query: 98  SAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTAD 157
           SAGTVT +YL+S G++WDEIDFEFLGN+SG PY +HTNVYT+G G++EQQF+LWFDPT D
Sbjct: 86  SAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGDKEQQFHLWFDPTVD 145

Query: 158 FHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLV 217
           FHTY  +WNP  ++F +DG PIREF+N E++G P+P  Q MRLY SLW A+ WATRGGL 
Sbjct: 146 FHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYASLWEAEHWATRGGLE 205

Query: 218 KTDWTKAPFTASFRSYNPNACVLSNGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYN 277
           KTDW+KAPFTA +R+YN +ACV SNG SSC   + W +Q LD   +  ++W Q+ YM+YN
Sbjct: 206 KTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANSSWFTQVLDFKGKNRVKWAQRKYMVYN 265

Query: 278 YCADKRRFPQGLPPECT 294
           YC DK+RFPQG PPEC+
Sbjct: 266 YCTDKKRFPQGAPPECS 282

BLAST of MC06g_new0095 vs. NCBI nr
Match: XP_022135082.1 (xyloglucan endotransglucosylase/hydrolase protein 22-like [Momordica charantia])

HSP 1 Score: 633 bits (1632), Expect = 2.03e-229
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG 60
           MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG
Sbjct: 1   MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG 60

Query: 61  NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF 120
           NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF
Sbjct: 61  NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF 120

Query: 121 LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE 180
           LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE
Sbjct: 121 LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE 180

Query: 181 FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS 240
           FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS
Sbjct: 181 FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS 240

Query: 241 NGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTKP 298
           NGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTKP
Sbjct: 241 NGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTKP 298

BLAST of MC06g_new0095 vs. NCBI nr
Match: XP_023003589.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita maxima])

HSP 1 Score: 495 bits (1275), Expect = 4.38e-175
Identity = 230/285 (80.70%), Postives = 258/285 (90.53%), Query Frame = 0

Query: 12  NNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRAS 71
           NNYS   L+L +LLPCL+GF  K AEAG+FYQ++D+TWGDGRGKI+ENGNLLTLSLD+AS
Sbjct: 8   NNYSM--LLLIVLLPCLLGFE-KMAEAGNFYQDMDLTWGDGRGKILENGNLLTLSLDKAS 67

Query: 72  GSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIV 131
           GSGFQSKKQYL GRFD+KIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSG+PYIV
Sbjct: 68  GSGFQSKKQYLNGRFDMKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGQPYIV 127

Query: 132 HTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPY 191
           HTN+Y QGQGNREQQFYLWFDPTADFHTYSFLWN A I+FYVDGRPIRE++N+ES G PY
Sbjct: 128 HTNIYIQGQGNREQQFYLWFDPTADFHTYSFLWNSARILFYVDGRPIREYKNMESNGVPY 187

Query: 192 PNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGAS-SCRNAA 251
           P KQ MRLY SLWNADDWATRGGLVKTDWTKAPFTA+FRSYN N CV +N AS  C+N+A
Sbjct: 188 PKKQAMRLYASLWNADDWATRGGLVKTDWTKAPFTAAFRSYNANGCVWTNAASWCCQNSA 247

Query: 252 PWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLP 295
           PW+S+ LD+  Q++LRWVQK YMIYNYCAD++RFPQGLP ECT+P
Sbjct: 248 PWMSEALDAGNQKVLRWVQKKYMIYNYCADRKRFPQGLPRECTVP 289

BLAST of MC06g_new0095 vs. NCBI nr
Match: XP_022933962.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita moschata])

HSP 1 Score: 493 bits (1270), Expect = 2.53e-174
Identity = 230/285 (80.70%), Postives = 257/285 (90.18%), Query Frame = 0

Query: 12  NNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRAS 71
           NNYS   L+L +LLPCL+GF  K AEAG+FYQ++DVTWGDGRGKI+ENGNLLTLSLD+AS
Sbjct: 8   NNYSM--LLLVILLPCLLGFE-KMAEAGNFYQDVDVTWGDGRGKILENGNLLTLSLDKAS 67

Query: 72  GSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIV 131
           GSGFQSKKQYL GRFD+KIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGN SG+PYIV
Sbjct: 68  GSGFQSKKQYLNGRFDMKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNSSGQPYIV 127

Query: 132 HTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPY 191
           HTN+Y QGQGNREQQFYLWFDPTADFHTYSFLWN A I+FYVDGRPIRE++N+ES G PY
Sbjct: 128 HTNIYIQGQGNREQQFYLWFDPTADFHTYSFLWNSARILFYVDGRPIREYKNMESNGVPY 187

Query: 192 PNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGAS-SCRNAA 251
           P KQ MRLY SLWNADDWATRGGLVKTDWTKAPFTA+FRSYN N CV +N AS  C+N+A
Sbjct: 188 PKKQAMRLYASLWNADDWATRGGLVKTDWTKAPFTAAFRSYNANGCVWTNAASWCCQNSA 247

Query: 252 PWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLP 295
           PW+S+ LD+  Q++LRWVQK YMIYNYCADK+RFP+GLP ECT+P
Sbjct: 248 PWMSEALDAGNQKVLRWVQKKYMIYNYCADKKRFPRGLPRECTVP 289

BLAST of MC06g_new0095 vs. NCBI nr
Match: XP_023531494.1 (probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 492 bits (1266), Expect = 1.03e-173
Identity = 228/285 (80.00%), Postives = 257/285 (90.18%), Query Frame = 0

Query: 12  NNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRAS 71
           NNYS   L+L +LLPCL+GF  K AEAG+FYQ++DVTWGDGRGKI++NGNLLTLSLD+AS
Sbjct: 8   NNYSM--LLLVILLPCLLGFE-KMAEAGNFYQDVDVTWGDGRGKILDNGNLLTLSLDKAS 67

Query: 72  GSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIV 131
           GSGFQSKKQYL GRFD+KIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSG+PYIV
Sbjct: 68  GSGFQSKKQYLNGRFDMKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGQPYIV 127

Query: 132 HTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPY 191
           HTN+Y QGQGNREQQFYLWFDPTADFHTYSFLWN A I+FYVDGRPIRE++N+ES G PY
Sbjct: 128 HTNIYIQGQGNREQQFYLWFDPTADFHTYSFLWNSARILFYVDGRPIREYKNMESNGVPY 187

Query: 192 PNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGAS-SCRNAA 251
           P KQ MRLY SLWNA+DWATRGG+VKTDWTKAPFTA+FRSYN N CV +N AS  C+N+A
Sbjct: 188 PKKQAMRLYASLWNAEDWATRGGVVKTDWTKAPFTAAFRSYNANGCVWTNAASWCCQNSA 247

Query: 252 PWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLP 295
           PW+S+ LD+  Q++LRWVQK YMIYNYC DK+RFPQGLP ECT+P
Sbjct: 248 PWMSEALDAGNQKVLRWVQKKYMIYNYCVDKKRFPQGLPRECTVP 289

BLAST of MC06g_new0095 vs. NCBI nr
Match: KAA0058292.1 (putative xyloglucan endotransglycosylase [Cucumis melo var. makuwa])

HSP 1 Score: 491 bits (1264), Expect = 2.40e-173
Identity = 227/297 (76.43%), Postives = 256/297 (86.20%), Query Frame = 0

Query: 1   MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG 60
           MAL  S+ +KL +Y    LVL ++    +G     A AG+FYQE+DVTWGDGRGKI+ENG
Sbjct: 1   MALLFSYSEKLTSYYSTILVLIII----VGVWAGEASAGNFYQEVDVTWGDGRGKIIENG 60

Query: 61  NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF 120
           NL+TLSLD+ASGSGFQSKKQYL+GRFD+KIKLVP NSAGTVTAYYLRS+GSSWDEIDFEF
Sbjct: 61  NLITLSLDKASGSGFQSKKQYLYGRFDMKIKLVPGNSAGTVTAYYLRSEGSSWDEIDFEF 120

Query: 121 LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE 180
           LGN+SG+PYIVHTN+Y  GQGNREQQFYLWFDPTADFHTYSFLWNPA IVFYVDGRPIRE
Sbjct: 121 LGNVSGQPYIVHTNIYVAGQGNREQQFYLWFDPTADFHTYSFLWNPARIVFYVDGRPIRE 180

Query: 181 FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS 240
           F+N+E+ G PYP KQ MR+Y SLWN +DWATRGGLVKTDWTKAPFTASFR Y PN CV +
Sbjct: 181 FKNMEANGAPYPKKQPMRVYASLWNGEDWATRGGLVKTDWTKAPFTASFRDYKPNGCVWT 240

Query: 241 NGAS-SCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPT 296
           N AS  C+N+APWLS+ LDS  Q+MLRWVQKNYMIYNYC DK+RFPQGLP ECT+PT
Sbjct: 241 NAASWCCQNSAPWLSEALDSGNQKMLRWVQKNYMIYNYCTDKKRFPQGLPKECTVPT 293

BLAST of MC06g_new0095 vs. ExPASy TrEMBL
Match: A0A6J1C1M9 (Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC111007154 PE=3 SV=1)

HSP 1 Score: 633 bits (1632), Expect = 9.82e-230
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 0

Query: 1   MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG 60
           MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG
Sbjct: 1   MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG 60

Query: 61  NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF 120
           NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF
Sbjct: 61  NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF 120

Query: 121 LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE 180
           LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE
Sbjct: 121 LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE 180

Query: 181 FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS 240
           FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS
Sbjct: 181 FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS 240

Query: 241 NGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTKP 298
           NGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTKP
Sbjct: 241 NGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTKP 298

BLAST of MC06g_new0095 vs. ExPASy TrEMBL
Match: A0A6J1KTR8 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497144 PE=3 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 2.12e-175
Identity = 230/285 (80.70%), Postives = 258/285 (90.53%), Query Frame = 0

Query: 12  NNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRAS 71
           NNYS   L+L +LLPCL+GF  K AEAG+FYQ++D+TWGDGRGKI+ENGNLLTLSLD+AS
Sbjct: 8   NNYSM--LLLIVLLPCLLGFE-KMAEAGNFYQDMDLTWGDGRGKILENGNLLTLSLDKAS 67

Query: 72  GSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIV 131
           GSGFQSKKQYL GRFD+KIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSG+PYIV
Sbjct: 68  GSGFQSKKQYLNGRFDMKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGQPYIV 127

Query: 132 HTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPY 191
           HTN+Y QGQGNREQQFYLWFDPTADFHTYSFLWN A I+FYVDGRPIRE++N+ES G PY
Sbjct: 128 HTNIYIQGQGNREQQFYLWFDPTADFHTYSFLWNSARILFYVDGRPIREYKNMESNGVPY 187

Query: 192 PNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGAS-SCRNAA 251
           P KQ MRLY SLWNADDWATRGGLVKTDWTKAPFTA+FRSYN N CV +N AS  C+N+A
Sbjct: 188 PKKQAMRLYASLWNADDWATRGGLVKTDWTKAPFTAAFRSYNANGCVWTNAASWCCQNSA 247

Query: 252 PWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLP 295
           PW+S+ LD+  Q++LRWVQK YMIYNYCAD++RFPQGLP ECT+P
Sbjct: 248 PWMSEALDAGNQKVLRWVQKKYMIYNYCADRKRFPQGLPRECTVP 289

BLAST of MC06g_new0095 vs. ExPASy TrEMBL
Match: A0A6J1F1B2 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111441216 PE=3 SV=1)

HSP 1 Score: 493 bits (1270), Expect = 1.22e-174
Identity = 230/285 (80.70%), Postives = 257/285 (90.18%), Query Frame = 0

Query: 12  NNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRAS 71
           NNYS   L+L +LLPCL+GF  K AEAG+FYQ++DVTWGDGRGKI+ENGNLLTLSLD+AS
Sbjct: 8   NNYSM--LLLVILLPCLLGFE-KMAEAGNFYQDVDVTWGDGRGKILENGNLLTLSLDKAS 67

Query: 72  GSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIV 131
           GSGFQSKKQYL GRFD+KIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGN SG+PYIV
Sbjct: 68  GSGFQSKKQYLNGRFDMKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNSSGQPYIV 127

Query: 132 HTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPY 191
           HTN+Y QGQGNREQQFYLWFDPTADFHTYSFLWN A I+FYVDGRPIRE++N+ES G PY
Sbjct: 128 HTNIYIQGQGNREQQFYLWFDPTADFHTYSFLWNSARILFYVDGRPIREYKNMESNGVPY 187

Query: 192 PNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGAS-SCRNAA 251
           P KQ MRLY SLWNADDWATRGGLVKTDWTKAPFTA+FRSYN N CV +N AS  C+N+A
Sbjct: 188 PKKQAMRLYASLWNADDWATRGGLVKTDWTKAPFTAAFRSYNANGCVWTNAASWCCQNSA 247

Query: 252 PWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLP 295
           PW+S+ LD+  Q++LRWVQK YMIYNYCADK+RFP+GLP ECT+P
Sbjct: 248 PWMSEALDAGNQKVLRWVQKKYMIYNYCADKKRFPRGLPRECTVP 289

BLAST of MC06g_new0095 vs. ExPASy TrEMBL
Match: A0A5A7UQY7 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006260 PE=3 SV=1)

HSP 1 Score: 491 bits (1264), Expect = 1.16e-173
Identity = 227/297 (76.43%), Postives = 256/297 (86.20%), Query Frame = 0

Query: 1   MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG 60
           MAL  S+ +KL +Y    LVL ++    +G     A AG+FYQE+DVTWGDGRGKI+ENG
Sbjct: 1   MALLFSYSEKLTSYYSTILVLIII----VGVWAGEASAGNFYQEVDVTWGDGRGKIIENG 60

Query: 61  NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF 120
           NL+TLSLD+ASGSGFQSKKQYL+GRFD+KIKLVP NSAGTVTAYYLRS+GSSWDEIDFEF
Sbjct: 61  NLITLSLDKASGSGFQSKKQYLYGRFDMKIKLVPGNSAGTVTAYYLRSEGSSWDEIDFEF 120

Query: 121 LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE 180
           LGN+SG+PYIVHTN+Y  GQGNREQQFYLWFDPTADFHTYSFLWNPA IVFYVDGRPIRE
Sbjct: 121 LGNVSGQPYIVHTNIYVAGQGNREQQFYLWFDPTADFHTYSFLWNPARIVFYVDGRPIRE 180

Query: 181 FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS 240
           F+N+E+ G PYP KQ MR+Y SLWN +DWATRGGLVKTDWTKAPFTASFR Y PN CV +
Sbjct: 181 FKNMEANGAPYPKKQPMRVYASLWNGEDWATRGGLVKTDWTKAPFTASFRDYKPNGCVWT 240

Query: 241 NGAS-SCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPT 296
           N AS  C+N+APWLS+ LDS  Q+MLRWVQKNYMIYNYC DK+RFPQGLP ECT+PT
Sbjct: 241 NAASWCCQNSAPWLSEALDSGNQKMLRWVQKNYMIYNYCTDKKRFPQGLPKECTVPT 293

BLAST of MC06g_new0095 vs. ExPASy TrEMBL
Match: A0A0A0LU38 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G422980 PE=3 SV=1)

HSP 1 Score: 485 bits (1248), Expect = 3.18e-171
Identity = 225/297 (75.76%), Postives = 253/297 (85.19%), Query Frame = 0

Query: 1   MALSCSFPKKLNNYSPDPLVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENG 60
           MAL  S+ K L NY      + LLL   +G     A AG+FY+E+DVTWGDGRGKI+ENG
Sbjct: 1   MALLFSYSKNLTNYYS----IILLLIFTVGVWAGEAPAGNFYKEVDVTWGDGRGKIIENG 60

Query: 61  NLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEF 120
           NL+TLSLD+ASGSGFQSKKQYL GRFD+KIKLVP NSAGTVTAYYLRS+GSSWDEIDFEF
Sbjct: 61  NLITLSLDKASGSGFQSKKQYLHGRFDMKIKLVPGNSAGTVTAYYLRSEGSSWDEIDFEF 120

Query: 121 LGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIRE 180
           LGN+SG+PY+VHTN+Y  GQGNREQQFYLWFDPTADFHTYSFLWNPA IVFYVDGRPIRE
Sbjct: 121 LGNVSGQPYVVHTNIYVGGQGNREQQFYLWFDPTADFHTYSFLWNPARIVFYVDGRPIRE 180

Query: 181 FRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLS 240
           F+N+E+ G PYP KQ MRLY SLWN +DWATRGGL+KTDWTKAPFTASFR+Y PN CV +
Sbjct: 181 FKNMEANGAPYPKKQPMRLYASLWNGEDWATRGGLIKTDWTKAPFTASFRAYKPNGCVWT 240

Query: 241 NGAS-SCRNAAPWLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPT 296
           N AS  C+N+APWLS+ LDS  Q+MLRWVQKNYMIYNYC DK+RFPQGLP EC +PT
Sbjct: 241 NAASWCCQNSAPWLSEALDSGNQKMLRWVQKNYMIYNYCTDKKRFPQGLPKECIVPT 293

BLAST of MC06g_new0095 vs. TAIR 10
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6 )

HSP 1 Score: 408.7 bits (1049), Expect = 4.1e-114
Identity = 184/276 (66.67%), Postives = 225/276 (81.52%), Query Frame = 0

Query: 23  LLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSKKQYL 82
           +++  L  F++ +  A +F +++++TWGDGRG+I  NG+LLTLSLD+ASGSGFQSK +YL
Sbjct: 9   IVVALLASFMICSVSA-NFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEYL 68

Query: 83  FGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGN 142
           FG+ D++IKLV  NSAGTVTAYYL+S GS+WDEIDFEFLGNLSG+PY +HTNV+TQG+G+
Sbjct: 69  FGKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKGD 128

Query: 143 REQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGS 202
           REQQF LWFDPT+DFHTYS LWNP  I+F VDG PIREF+N+ES GT +P  Q MR+Y S
Sbjct: 129 REQQFKLWFDPTSDFHTYSILWNPQRIIFSVDGTPIREFKNMESQGTLFPKNQPMRMYSS 188

Query: 203 LWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGASSCRNAA------PWLSQE 262
           LWNA++WATRGGLVKTDW+KAPFTAS+R +N  ACV+ NG SSC N +       WLSQE
Sbjct: 189 LWNAEEWATRGGLVKTDWSKAPFTASYRGFNEEACVVINGQSSCPNVSGQGSTGSWLSQE 248

Query: 263 LDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPEC 293
           LDS  Q  +RWVQ NYMIYNYC D +RFPQGLP EC
Sbjct: 249 LDSTGQEQMRWVQNNYMIYNYCTDAKRFPQGLPREC 283

BLAST of MC06g_new0095 vs. TAIR 10
Match: AT5G57560.1 (Xyloglucan endotransglucosylase/hydrolase family protein )

HSP 1 Score: 408.7 bits (1049), Expect = 4.1e-114
Identity = 180/281 (64.06%), Postives = 226/281 (80.43%), Query Frame = 0

Query: 19  LVLFLLLPCLMGFVLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSK 78
           + +  LLP  +  ++ ++ + +F +++++TWGDGRG+I  NG LLTLSLD++SGSGFQSK
Sbjct: 1   MAITYLLPLFLSLIITSSVSANFQRDVEITWGDGRGQIKNNGELLTLSLDKSSGSGFQSK 60

Query: 79  KQYLFGRFDLKIKLVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQ 138
            +YLFG+  +++KLVP NSAGTVT  YL+S G++WDEIDFEFLGN SGEPY +HTNVYTQ
Sbjct: 61  NEYLFGKVSMQMKLVPGNSAGTVTTLYLKSPGTTWDEIDFEFLGNSSGEPYTLHTNVYTQ 120

Query: 139 GQGNREQQFYLWFDPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMR 198
           G+G++EQQF LWFDPTA+FHTY+ LWNP  I+F VDG PIREF+N+ES+GT +P  + MR
Sbjct: 121 GKGDKEQQFKLWFDPTANFHTYTILWNPQRIIFTVDGTPIREFKNMESLGTLFPKNKPMR 180

Query: 199 LYGSLWNADDWATRGGLVKTDWTKAPFTASFRSYNPNACVLSNGASSCRNAA-------P 258
           +Y SLWNADDWATRGGLVKTDW+KAPFTAS+R +   ACV SNG SSC NA+        
Sbjct: 181 MYSSLWNADDWATRGGLVKTDWSKAPFTASYRGFQQEACVWSNGKSSCPNASKQGTTTGS 240

Query: 259 WLSQELDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPEC 293
           WLSQELDS  Q+ +RWVQ+NYMIYNYC D +RFPQGLP EC
Sbjct: 241 WLSQELDSTAQQRMRWVQRNYMIYNYCTDAKRFPQGLPKEC 281

BLAST of MC06g_new0095 vs. TAIR 10
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25 )

HSP 1 Score: 403.3 bits (1035), Expect = 1.7e-112
Identity = 179/256 (69.92%), Postives = 212/256 (82.81%), Query Frame = 0

Query: 38  AGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPANS 97
           AG+F  E D+TWGDGRGK++ NG LLTLSLDRASGSGFQ+KK+YLFG+ D+++KLVP NS
Sbjct: 27  AGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPGNS 86

Query: 98  AGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTADF 157
           AGTVTAYYL+S+G +WDEIDFEFLGNL+G+PY +HTNVYTQG+G+REQQF+LWFDPTADF
Sbjct: 87  AGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYTQGKGDREQQFHLWFDPTADF 146

Query: 158 HTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLVK 217
           HTYS LWNP HIVF VD  P+REF+N++ MG  YP  Q MRLY SLWNAD WATRGGLVK
Sbjct: 147 HTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRLYSSLWNADQWATRGGLVK 206

Query: 218 TDWTKAPFTASFRSYNPNACVLSNGASSCRNAAP-WLSQELDSATQRMLRWVQKNYMIYN 277
           TDW+KAPFTAS+R++  +ACV S G SSC   +P W SQ LD   +  +R VQ+ YMIYN
Sbjct: 207 TDWSKAPFTASYRNFRADACVSSGGRSSCPAGSPRWFSQRLDLTAEDKMRVVQRKYMIYN 266

Query: 278 YCADKRRFPQGLPPEC 293
           YC D +RFPQG P EC
Sbjct: 267 YCTDTKRFPQGFPKEC 282

BLAST of MC06g_new0095 vs. TAIR 10
Match: AT2G18800.1 (xyloglucan endotransglucosylase/hydrolase 21 )

HSP 1 Score: 391.3 bits (1004), Expect = 6.7e-109
Identity = 177/283 (62.54%), Postives = 220/283 (77.74%), Query Frame = 0

Query: 32  VLKTAEAGSFYQELDVTWGDGRGKIMENGNLLTLSLDRASGSGFQSKKQYLFGRFDLKIK 91
           +L       F Q++D+TWGDGRG I+ NG LL L LD++SGSGFQSK +YL+G+ D++IK
Sbjct: 19  ILLVVHGKDFNQDIDITWGDGRGNILNNGTLLNLGLDQSSGSGFQSKAEYLYGKVDMQIK 78

Query: 92  LVPANSAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGNREQQFYLWF 151
           LVP NSAGTVT +YL+SQG +WDEIDFEFLGN+SG+PYIVHTNVYTQG+G+REQQFYLWF
Sbjct: 79  LVPGNSAGTVTTFYLKSQGLTWDEIDFEFLGNVSGDPYIVHTNVYTQGKGDREQQFYLWF 138

Query: 152 DPTADFHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGSLWNADDWAT 211
           DPTA FH YS LWNP+HIVFY+DG+PIREF+N+E +G  YP  Q MR+YGSLWNADDWAT
Sbjct: 139 DPTAAFHNYSILWNPSHIVFYIDGKPIREFKNLEVLGVAYPKNQPMRMYGSLWNADDWAT 198

Query: 212 RGGLVKTDWTKAPFTASFRSYN-PNACVLS--NG-------------ASSCRNAAPWLSQ 271
           RGGLVKT+W++ PF ASF +YN  NACV S  NG             +SS  + + W SQ
Sbjct: 199 RGGLVKTNWSQGPFVASFMNYNSENACVWSIVNGTTTTSPCSPGDSTSSSSSSTSEWFSQ 258

Query: 272 E-LDSATQRMLRWVQKNYMIYNYCADKRRFPQGLPPECTLPTK 298
             +DS+++++LRWVQ+ +M+YNYC DK+RF  GLP ECT   K
Sbjct: 259 RGMDSSSKKVLRWVQRKFMVYNYCKDKKRFSNGLPVECTAKNK 301

BLAST of MC06g_new0095 vs. TAIR 10
Match: AT5G48070.1 (xyloglucan endotransglucosylase/hydrolase 20 )

HSP 1 Score: 379.4 bits (973), Expect = 2.6e-105
Identity = 160/257 (62.26%), Postives = 210/257 (81.71%), Query Frame = 0

Query: 38  AGSFYQELDVTWGDGRGKIMEN-GNLLTLSLDRASGSGFQSKKQYLFGRFDLKIKLVPAN 97
           AGSF++++ + WGDGRGKI++N GNLL+LSLD+ SGSGFQS +++L+G+ ++++KLVP N
Sbjct: 26  AGSFHKDVQIHWGDGRGKILDNVGNLLSLSLDKFSGSGFQSHQEFLYGKVEVQMKLVPGN 85

Query: 98  SAGTVTAYYLRSQGSSWDEIDFEFLGNLSGEPYIVHTNVYTQGQGNREQQFYLWFDPTAD 157
           SAGTVT +YL+S G++WDEIDFEFLGN+SG PY +HTNVYT+G G++EQQF+LWFDPT D
Sbjct: 86  SAGTVTTFYLKSPGTTWDEIDFEFLGNISGHPYTLHTNVYTKGTGDKEQQFHLWFDPTVD 145

Query: 158 FHTYSFLWNPAHIVFYVDGRPIREFRNVESMGTPYPNKQEMRLYGSLWNADDWATRGGLV 217
           FHTY  +WNP  ++F +DG PIREF+N E++G P+P  Q MRLY SLW A+ WATRGGL 
Sbjct: 146 FHTYCIIWNPQRVIFTIDGIPIREFKNSEALGVPFPKHQPMRLYASLWEAEHWATRGGLE 205

Query: 218 KTDWTKAPFTASFRSYNPNACVLSNGASSCRNAAPWLSQELDSATQRMLRWVQKNYMIYN 277
           KTDW+KAPFTA +R+YN +ACV SNG SSC   + W +Q LD   +  ++W Q+ YM+YN
Sbjct: 206 KTDWSKAPFTAFYRNYNVDACVWSNGKSSCSANSSWFTQVLDFKGKNRVKWAQRKYMVYN 265

Query: 278 YCADKRRFPQGLPPECT 294
           YC DK+RFPQG PPEC+
Sbjct: 266 YCTDKKRFPQGAPPECS 282

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q388575.7e-11364.06Xyloglucan endotransglucosylase/hydrolase protein 22 OS=Arabidopsis thaliana OX=... [more]
Q389105.7e-11366.67Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis tha... [more]
Q389072.4e-11169.92Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis tha... [more]
Q9ZV409.5e-10862.54Probable xyloglucan endotransglucosylase/hydrolase protein 21 OS=Arabidopsis tha... [more]
Q9FI313.7e-10462.26Xyloglucan endotransglucosylase/hydrolase protein 20 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
XP_022135082.12.03e-229100.00xyloglucan endotransglucosylase/hydrolase protein 22-like [Momordica charantia][more]
XP_023003589.14.38e-17580.70probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita maxima][more]
XP_022933962.12.53e-17480.70probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita moschat... [more]
XP_023531494.11.03e-17380.00probable xyloglucan endotransglucosylase/hydrolase protein 23 [Cucurbita pepo su... [more]
KAA0058292.12.40e-17376.43putative xyloglucan endotransglycosylase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1C1M99.82e-230100.00Xyloglucan endotransglucosylase/hydrolase OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1KTR82.12e-17580.70Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F1B21.22e-17480.70Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5A7UQY71.16e-17376.43Xyloglucan endotransglucosylase/hydrolase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A0A0LU383.18e-17175.76Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G42... [more]
Match NameE-valueIdentityDescription
AT4G25810.14.1e-11466.67xyloglucan endotransglycosylase 6 [more]
AT5G57560.14.1e-11464.06Xyloglucan endotransglucosylase/hydrolase family protein [more]
AT5G57550.11.7e-11269.92xyloglucan endotransglucosylase/hydrolase 25 [more]
AT2G18800.16.7e-10962.54xyloglucan endotransglucosylase/hydrolase 21 [more]
AT5G48070.12.6e-10562.26xyloglucan endotransglucosylase/hydrolase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008264Beta-glucanasePRINTSPR00737GLHYDRLASE16coord: 131..144
score: 51.19
coord: 149..166
score: 51.85
coord: 73..91
score: 21.05
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 43..222
e-value: 7.4E-60
score: 201.5
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 35..229
score: 30.118618
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 248..292
e-value: 9.0E-21
score: 73.5
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 15..297
e-value: 2.6E-114
score: 379.3
IPR016455Xyloglucan endotransglucosylase/hydrolaseCDDcd02176GH16_XETcoord: 38..292
e-value: 2.57557E-152
score: 424.689
NoneNo IPR availableGENE3D2.60.120.200coord: 29..295
e-value: 4.8E-106
score: 356.1
NoneNo IPR availablePANTHERPTHR31062:SF153XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 21-RELATEDcoord: 15..293
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 15..293
IPR008263Glycoside hydrolase, family 16, active sitePROSITEPS01034GH16_1coord: 115..125
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 42..295

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0095.1MC06g_new0095.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0042545 cell wall modification
biological_process GO:0080022 primary root development
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity