MC06g_new0053 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0053
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionFormin-like protein
LocationMC06: 1701803 .. 1705073 (-)
RNA-Seq ExpressionMC06g_new0053
SyntenyMC06g_new0053
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCAGAGACTGTATGATTGGACTTGCTGATTTGGCAATTATATATAATATTTTTTACTTTTTTGGTAATTGGAGGTGTGGTCCTCTTCCTTTCTTCTTTTTTCCTCGTTGACCTCCGCCGGCGCCGCCGTCTCCCTCAGATCTAACGACGGGGGAGCTTCCCCTTCGTTTTAGACTGGGGGTTCTCAGGACGACGGTTGATCGATGACCTCAGGATCTTTCTCTAGGTTAAACCCGCCTCTGAAATCCGCCGCTGGTTTAGCAGAAGAAACGATGGGAATCGAGCAGCACCTGGGAGAAACTGCTCTCCAAGATACAAAAGCTCCATGGACTATGCCTAATCCAACCATGCCATTCTCAAAATCGCGATGGGATCTCGTTTTATCTGGACAGAAAACAAGGCGGCGAAGAGCGAAAGGCTGATGGTGGGCTTCTTTTTTTCGATGAAATTCTCCATTTCCGGCCAAATTTTTTATTCAATCCCTTTAAATTTTCGCCTCCAACCTCAAAATCTTCAGTAGAATGCCTCTCTGCACCAGTTTACAGTGGTGGTACCACACCACTCTGTAATCGAGTCAGGTTTAACAGATTCTCTCTTTTTGCTACCTATCAATATGGATATCTTGATATCAACTTGTGAGATGATTTTTAGTGGTGTCATTTGGTAACTTATTCTGGTTATTATTTGTTTTCAGTGTTTGATTGTTGCTTCTCCACGGAAGTGTTGGAAGAAGAAGCATATAAGGTCTACTTGGATGGCATTGTAGCACAGTTACAGAACCACTTTTCTGATGCTTCTTTCATGGGTTTCAACTTCAGAGAAGGTGATAGGCAAAGCCAAATTTCTAATATATTGACTCAGTATGACATGACAGTTTTGGATTATCCTGGGCATTATGAGGGATGTCCTTTATTGTCATTGGAGATGATCTTCCTTCGATCAAGTGAAAGCTGGTTGTCACTGGATGGTCAACAGAATGTGGTACTGATGCACTGTGAAAGAGGAGGCTGGCCTGTTCTTGCATTCATGCTAGCTGGTCTTCTGTTGTACAGGAAACAATACAGTGGGGAGCAGAAGACTCTTGAAATGGTCTACAAGCAAGCTCCTAAGGAACTTCTCCATCTTTTGTCTCCTTTAAACCCTCAACCTTCCCACCTGAGATATCTTCAATACATTTCCAGGAGAAATTTGGATTCTGATTGGCCTCCATCTGATACACCTCTGGTTTTGGATTGTTTAATAATTAGAGTTATACCTCTCTTTGATGGGGGAAAAGGATGCAGGCATGTTGTGCATGTCTATGGTCAAGACCCCTCAGCTCCGGCCAACAGAACTTTGAAGCTTTTATTTTCGACTCCAATCAATAAGAAACATATCCGCAGCTACTTACAGGTAATGGCTCAGCAACCCAAATTCTGAAAGCTGAAATTATTCAACATTTAAGTTTTCTTATTAATTGTTTGTTACCACAGGCAGAGTGTATGCTGGTGAAAGTTGACATTCGTTGCCGCGTGCAAGGGAATGTGGTTGTTGAATGCATCCATTTGGATGAAGATTTAGTAGGTGAGGAGATGGTATTTAGATTTATGTTTCACACAGCATTTGTTAGATCAAACATTATGATGCTGAGCTGTGATGAAGTTGATGTTGCATGGGATGCAAGGGACCAGTTCTCTAAGGACTTCAGAGTTGAGGTAAGTTATTGGTCTTCTTTTATGAATGTGCTTTCGTGAAGTTGTGAAAGCTGACACAAGATTGGATTTATGTTAGGCACTCTTTTTGGATGCTGATGCTGTTGTCCCCAATCTCGCCACAGCCCTTGACGATGAAGATGAATATGAAACAGGAGCTGCTTCTTTTGAAGAGTTTATTGAGGTTGAAGAGATCTTCAACAATGTAATGGATGGGCAGGAAGCCAAAGGGTCCAATGATCCTCCTGCATTCCAAGATTGTGCATCTGCCGATGGAAATTTTAAGCATGATAAGAAATCCGATTTTGATTCAGTGAAGGACATTACTGTGGATGATGTGAAATACAAACTGGATGAAAATATATACTCTGAGCTTAATGCAGTTAAGAACACTGTTGTGGAAGAGGACATGGATCCTCCATTCAGTAGACAACCACCACCATGGCTGCAAATCTATTCGTCCGTTTCTACTGTAATGGTGGCTGGTTCTATTCCACCTCCACCACCCCCACCTCCACCAATGGTGCATAAGGCATTTCCCTCACCATCTCTAGTGAGAGGAACACCACCTCCACCTCCTTCAAAGCATGGAGTACCACCTCCGCCACCACCTCCAAAAGAGTGCTTGTATTTTGGTCAATTGAAACACCAAAAAAGAACATCCATAAGTCAAAAGTACAAACTATAAACTCATATCTTCGAAGCTATGAATTCGAATCTACCCATCTAGGATATCAAATCTCTTCCGAATTTTTTGATGTTGAAAGTGATATAGGGTAATTTAGTTGTTTCATGAAATTAGGTTGTGTGTGCAAAAGCCAAACATTTGGAAATCTGCAAAAATGCATTGTGGGTAGAATTCTCTGAGAAAGACAGAGATTATCTCATATCCAATTTGGTGACTTGGGTTTCAGTGAATCATTTTAAAGAGTTCAGGATTCATTAAGTTAGAGGGTCAAAGCCAGGACCAGAACCATTGGCTATGAACAGTGATTCTTCATCTTCTCCATTGTCTAGTTTTATCATCTCTCCTCTCCAATATTTACATGATGTGTCTGGACTGAATATTTTGTTGCAGACAAGGAACTTTACATAATTGAAATGGCTGTTTATGGAGATGTAATCCAGTCAGAGAACCAGTGTCGTTATAGAACAGTTTCAGAAATTATGTGGCTATATAAATCAAAGGACCACCTCGCCCCTGCAAGTTGCCATAATGAAGATTCTTTGCAACAAAAAGAAGAGTTGAAAGTGTAGCAAGTTTTCCCATATGTAATAGCTCTATAATTTGTAATGCAATGACTTGATTGCATACAATGTCACATGTTTGAAATATGGATGAATCTAATTAATTTGAAAGACTTAAATAATTGTAGAATTATCTAAACTACTCTGCAATTTTTTTTAATTTTATTGCTGGTTTGTGGTTCTTTTAAAGGTGTTTCTCTTCTCCATTGTCTTGGTTTGATATCTAGAAGTAGAAGGGCATCTATTGCAGCCTTTACATTTCTTTGCACTTTATTTGTAATCATTGAGGCCATAACGGTCCCCTCTCACTTCCATTATTCAATGAATCATCC

mRNA sequence

ATGGGTTTCAACTTCAGAGAAGGTGATAGGCAAAGCCAAATTTCTAATATATTGACTCAGTATGACATGACAGTTTTGGATTATCCTGGGCATTATGAGGGATGTCCTTTATTGTCATTGGAGATGATCTTCCTTCGATCAAGTGAAAGCTGGTTGTCACTGGATGGTCAACAGAATGTGGTACTGATGCACTGTGAAAGAGGAGGCTGGCCTGTTCTTGCATTCATGCTAGCTGGTCTTCTGTTGTACAGGAAACAATACAGTGGGGAGCAGAAGACTCTTGAAATGGTCTACAAGCAAGCTCCTAAGGAACTTCTCCATCTTTTGTCTCCTTTAAACCCTCAACCTTCCCACCTGAGATATCTTCAATACATTTCCAGGAGAAATTTGGATTCTGATTGGCCTCCATCTGATACACCTCTGGTTTTGGATTGTTTAATAATTAGAGTTATACCTCTCTTTGATGGGGGAAAAGGATGCAGGCATGTTGTGCATGTCTATGGTCAAGACCCCTCAGCTCCGGCCAACAGAACTTTGAAGCTTTTATTTTCGACTCCAATCAATAAGAAACATATCCGCAGCTACTTACAGGCAGAGTGTATGCTGGTGAAAGTTGACATTCGTTGCCGCGTGCAAGGGAATGTGGTTGTTGAATGCATCCATTTGGATGAAGATTTAGTAGGTGAGGAGATGGTATTTAGATTTATGTTTCACACAGCATTTGTTAGATCAAACATTATGATGCTGAGCTGTGATGAAGTTGATGTTGCATGGGATGCAAGGGACCAGTTCTCTAAGGACTTCAGAGTTGAGGCACTCTTTTTGGATGCTGATGCTGTTGTCCCCAATCTCGCCACAGCCCTTGACGATGAAGATGAATATGAAACAGGAGCTGCTTCTTTTGAAGAGTTTATTGAGGTTGAAGAGATCTTCAACAATGTAATGGATGGGCAGGAAGCCAAAGGGTCCAATGATCCTCCTGCATTCCAAGATTGTGCATCTGCCGATGGAAATTTTAAGCATGATAAGAAATCCGATTTTGATTCAGTGAAGGACATTACTGTGGATGATGTGAAATACAAACTGGATGAAAATATATACTCTGAGCTTAATGCAGTTAAGAACACTGTTGTGGAAGAGGACATGGATCCTCCATTCAGTAGACAACCACCACCATGGCTGCAAATCTATTCGTCCGTTTCTACTGTAATGGTGGCTGGTTCTATTCCACCTCCACCACCCCCACCTCCACCAATGGTGCATAAGGCATTTCCCTCACCATCTCTAGTGAGAGGAACACCACCTCCACCTCCTTCAAAGCATGGAGTACCACCTCCGCCACCACCTCCAAAAGAGTGCTTGTATTTTGGTCAATTGAAACACCAAAAAAGAACATCCATAAGTCAAAAGTACAAACTATAA

Coding sequence (CDS)

ATGGGTTTCAACTTCAGAGAAGGTGATAGGCAAAGCCAAATTTCTAATATATTGACTCAGTATGACATGACAGTTTTGGATTATCCTGGGCATTATGAGGGATGTCCTTTATTGTCATTGGAGATGATCTTCCTTCGATCAAGTGAAAGCTGGTTGTCACTGGATGGTCAACAGAATGTGGTACTGATGCACTGTGAAAGAGGAGGCTGGCCTGTTCTTGCATTCATGCTAGCTGGTCTTCTGTTGTACAGGAAACAATACAGTGGGGAGCAGAAGACTCTTGAAATGGTCTACAAGCAAGCTCCTAAGGAACTTCTCCATCTTTTGTCTCCTTTAAACCCTCAACCTTCCCACCTGAGATATCTTCAATACATTTCCAGGAGAAATTTGGATTCTGATTGGCCTCCATCTGATACACCTCTGGTTTTGGATTGTTTAATAATTAGAGTTATACCTCTCTTTGATGGGGGAAAAGGATGCAGGCATGTTGTGCATGTCTATGGTCAAGACCCCTCAGCTCCGGCCAACAGAACTTTGAAGCTTTTATTTTCGACTCCAATCAATAAGAAACATATCCGCAGCTACTTACAGGCAGAGTGTATGCTGGTGAAAGTTGACATTCGTTGCCGCGTGCAAGGGAATGTGGTTGTTGAATGCATCCATTTGGATGAAGATTTAGTAGGTGAGGAGATGGTATTTAGATTTATGTTTCACACAGCATTTGTTAGATCAAACATTATGATGCTGAGCTGTGATGAAGTTGATGTTGCATGGGATGCAAGGGACCAGTTCTCTAAGGACTTCAGAGTTGAGGCACTCTTTTTGGATGCTGATGCTGTTGTCCCCAATCTCGCCACAGCCCTTGACGATGAAGATGAATATGAAACAGGAGCTGCTTCTTTTGAAGAGTTTATTGAGGTTGAAGAGATCTTCAACAATGTAATGGATGGGCAGGAAGCCAAAGGGTCCAATGATCCTCCTGCATTCCAAGATTGTGCATCTGCCGATGGAAATTTTAAGCATGATAAGAAATCCGATTTTGATTCAGTGAAGGACATTACTGTGGATGATGTGAAATACAAACTGGATGAAAATATATACTCTGAGCTTAATGCAGTTAAGAACACTGTTGTGGAAGAGGACATGGATCCTCCATTCAGTAGACAACCACCACCATGGCTGCAAATCTATTCGTCCGTTTCTACTGTAATGGTGGCTGGTTCTATTCCACCTCCACCACCCCCACCTCCACCAATGGTGCATAAGGCATTTCCCTCACCATCTCTAGTGAGAGGAACACCACCTCCACCTCCTTCAAAGCATGGAGTACCACCTCCGCCACCACCTCCAAAAGAGTGCTTGTATTTTGGTCAATTGAAACACCAAAAAAGAACATCCATAAGTCAAAAGTACAAACTATAA

Protein sequence

MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAASFEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMVHKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL
Homology
BLAST of MC06g_new0053 vs. ExPASy Swiss-Prot
Match: Q9FLQ7 (Formin-like protein 20 OS=Arabidopsis thaliana OX=3702 GN=FH20 PE=2 SV=3)

HSP 1 Score: 510.8 bits (1314), Expect = 1.7e-143
Identity = 259/407 (63.64%), Postives = 310/407 (76.17%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREG+++SQIS++L+QYDMTV+DYP  YE CPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGEQRSQISDVLSQYDMTVMDYPRQYESCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFML+GLLLYRKQY GEQKTLEMV+KQAPKELLHLLSPLNPQPS 
Sbjct: 121 NVLLMHCERGGWPVLAFMLSGLLLYRKQYHGEQKTLEMVHKQAPKELLHLLSPLNPQPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPL+LDCLI+R +P F+G KGCR ++ VYGQDP A  NR+
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLLLDCLILRDLPHFEGKKGCRPILRVYGQDPKARTNRS 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
             LLFST   KKH R Y Q EC+LVK+DI+CRVQG+VV+ECIHL +DLV EEMVFR MFH
Sbjct: 241 SILLFSTLKTKKHTRLYQQEECILVKLDIQCRVQGDVVLECIHLHDDLVSEEMVFRIMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAFVR+NI+ML  DE+D+ WD +DQF K+F+ E LF  ADAVVP + T+   +DE +   
Sbjct: 301 TAFVRANILMLQRDEMDILWDVKDQFPKEFKAEVLFSGADAVVPPITTSTLSDDENDFDM 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEA-----------------------KGSNDPPAFQDCASA 360
            S EEF EVEEIF++V+DG +                        KG  +P AF DCAS 
Sbjct: 361 TSPEEFFEVEEIFSDVIDGPDHKRDSDSFVVVDTASDDSEGKEVWKGDVEPNAFLDCASD 420

Query: 361 DGNFKHD--KKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 381
           D N KHD   ++  D VKDITVDDV+Y+ D    S +++VK+  +++
Sbjct: 421 DSNHKHDMHAETSTDPVKDITVDDVQYRSDGKADSNIDSVKDIGIDD 467

BLAST of MC06g_new0053 vs. ExPASy Swiss-Prot
Match: Q6ZCX3 (Formin-like protein 6 OS=Oryza sativa subsp. japonica OX=39947 GN=FH6 PE=2 SV=2)

HSP 1 Score: 383.6 bits (984), Expect = 3.1e-105
Identity = 184/316 (58.23%), Postives = 239/316 (75.63%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREG+ QS ++NIL+ Y+M V+DYP  YEGCPL+++EMI  FLRS ESWLSL  QQ
Sbjct: 61  MVFNFREGESQSLLANILSSYEMVVMDYPRQYEGCPLVTIEMIHHFLRSGESWLSL-SQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV++MHCERGGW VLAFMLAGLLLYRKQY GEQ+TLEM+Y+QAP+EL+ LLSPLNP PS 
Sbjct: 121 NVLIMHCERGGWAVLAFMLAGLLLYRKQYIGEQRTLEMIYRQAPRELIQLLSPLNPIPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           +RYL YISRRN+ + WPP D  L LDC+I+R IP F+G  GCR +  +YG+DP    + T
Sbjct: 181 IRYLHYISRRNVSAVWPPGDRALTLDCVILRNIPGFNGEGGCRPIFRIYGKDPLLATSNT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            K+LFSTP   K++R Y + +C L+K+DI C +QG+VV+ECI LD D   EEM+FR MF+
Sbjct: 241 PKVLFSTPKRSKYVRLYKKVDCELIKIDIHCHIQGDVVLECISLDADQQREEMIFRVMFN 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAF+RSNI+ML+ DE+D+ WDA+D+F K+FR E LF + D+V  N   +++     E   
Sbjct: 301 TAFIRSNILMLNRDEIDILWDAKDRFPKEFRAEVLFSEMDSV--NQLDSMEVGGIGEKEG 360

Query: 301 ASFEEFIEVEEIFNNV 315
              E F +V+E+F+NV
Sbjct: 361 LPVEAFAKVQEMFSNV 373


HSP 2 Score: 27.7 bits (60), Expect = 4.4e+02
Identity = 27/63 (42.86%), Postives = 30/63 (47.62%), Query Frame = 0

Query: 390 PPPWLQIYSS--VSTVMVAGSIPPPPPPPPPMVHKAFPSPSLVRGTPPPPPSKHGVPPPP 449
           PPP LQ  S+   S V    S PPPP P    V  + P P      P   PS+   PPPP
Sbjct: 738 PPPPLQSPSTPRCSPVRTLASPPPPPAPTSSPVRMSGPPPPPPPPAPNSCPSRPAPPPPP 797

Query: 450 PPP 451
           PPP
Sbjct: 798 PPP 800

BLAST of MC06g_new0053 vs. ExPASy Swiss-Prot
Match: Q9SK28 (Formin-like protein 18 OS=Arabidopsis thaliana OX=3702 GN=FH18 PE=2 SV=3)

HSP 1 Score: 364.4 bits (934), Expect = 2.0e-99
Identity = 176/314 (56.05%), Postives = 229/314 (72.93%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFR+GD +S++ ++LT+YDMT++DYP HYEGCPLL++E +  FL+S+ESWL L  QQ
Sbjct: 61  MVFNFRDGDSRSRMESVLTEYDMTIMDYPRHYEGCPLLTMETVHHFLKSAESWLLL-SQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           N++L HCE GGWP LAFMLA LLLYRKQ+SGE +TLEM+YKQAP+ELL L+SPLNP PS 
Sbjct: 121 NILLSHCELGGWPTLAFMLASLLLYRKQFSGEHRTLEMIYKQAPRELLQLMSPLNPLPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LR+LQYISRRN+ S WPP D  L LDC+ +R+IP FDG  GCR +  +YGQDP   ++RT
Sbjct: 181 LRFLQYISRRNVGSQWPPLDQALTLDCVNLRLIPDFDGEGGCRPIFRIYGQDPFMASDRT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            K+LFS P   K +R Y QA+C LVK+DI C + G+VV+ECI L  DL  EEM+FR +F+
Sbjct: 241 SKVLFSMPKRSKAVRQYKQADCELVKIDINCHILGDVVLECITLGSDLEREEMMFRVVFN 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAF+RSNI+ L+  E+DV W+  D+F KDF  E +F +  A       ++D     E   
Sbjct: 301 TAFLRSNILTLNRGEIDVLWNTTDRFPKDFSAEVIFSEMGA--GKKLASVDLPHMEEKDV 360

Query: 301 ASFEEFIEVEEIFN 313
              E F +V+EIF+
Sbjct: 361 LPMEAFAKVQEIFS 371


HSP 2 Score: 33.9 bits (76), Expect = 6.1e+00
Identity = 33/76 (43.42%), Postives = 40/76 (52.63%), Query Frame = 0

Query: 384 PPFSRQPPPWLQIYSSVSTVMVAGSI----PPPPPPPPPM-VHKAFPSPSLVRGTPPPPP 443
           PP    PPP   + S+ S    + SI    PPPPPPPPP+  H++  S S +   PP PP
Sbjct: 604 PPPPPPPPPISSLRSTPSPSSTSNSIATQGPPPPPPPPPLQSHRSALSSSPL--PPPLPP 663

Query: 444 SK----HGVPPPPPPP 451
            K       PPPPPPP
Sbjct: 664 KKLLATTNPPPPPPPP 677

BLAST of MC06g_new0053 vs. ExPASy Swiss-Prot
Match: Q84ZL0 (Formin-like protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=FH5 PE=2 SV=2)

HSP 1 Score: 350.9 bits (899), Expect = 2.2e-95
Identity = 183/369 (49.59%), Postives = 256/369 (69.38%), Query Frame = 0

Query: 4   NFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQNVV 63
           NF  GD++S+IS+IL++YDMTV+DYP  YEGCPLL LEMI  FL+S E+WLS++GQ N++
Sbjct: 64  NFWSGDKRSRISDILSEYDMTVMDYPQQYEGCPLLQLEMIHHFLKSCENWLSVEGQHNML 123

Query: 64  LMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLRY 123
           LMHCERGGWPVLAFMLAGLLLYRK Y+GEQKTLEMVYKQA ++ +    PLNPQ SH+RY
Sbjct: 124 LMHCERGGWPVLAFMLAGLLLYRKTYTGEQKTLEMVYKQARRDFIQQFFPLNPQSSHMRY 183

Query: 124 LQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLKL 183
           L YI+R+    + PP   PL+LD +++ V+P FD   GCR  + V+GQD S+ +N++ K+
Sbjct: 184 LHYITRQGSGPEKPPISRPLILDSIVLHVVPRFDAEGGCRPYLRVHGQD-SSSSNKSAKV 243

Query: 184 LFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTAF 243
           L+  P  KKH++ Y QAE + VKV   CRVQG+VV+ECIH+ ++L  EE++FR MF+TAF
Sbjct: 244 LYEMPKTKKHLQRYGQAE-VPVKVGAFCRVQGDVVLECIHIGDNLDHEEIMFRVMFNTAF 303

Query: 244 VRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALD-DEDEYETGAAS 303
           ++SNI+ L+ D++DV+W++ +QF +DFR E +F D  +  P  AT  + D+D  ET  AS
Sbjct: 304 IQSNILGLNRDDIDVSWNSNNQFPRDFRAEVVFSDPGSFKPAAATVEEVDDDGDETDVAS 363

Query: 304 F---EEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDD 363
               EEF E EE +++     E + ++   +  D A  DG    +     +  K    +D
Sbjct: 364 VDTGEEFYEAEEDWHDARRDPETQSTDGRTSIGD-AELDGGVSREDSGSLE--KHRADED 423

Query: 364 VKYKLDENI 367
           VK  + +N+
Sbjct: 424 VKIVISQNL 427


HSP 2 Score: 33.5 bits (75), Expect = 7.9e+00
Identity = 23/44 (52.27%), Postives = 26/44 (59.09%), Query Frame = 0

Query: 410 PPPPPPPPPMVHKAFPS---PSLVRGTPPPPPSKHGVPPPPPPP 451
           PPPPPPPPP    +  S    S  +  PPPPP    +PPPPPPP
Sbjct: 826 PPPPPPPPPYASSSSLSMHMGSATKQQPPPPPPPPPLPPPPPPP 869

BLAST of MC06g_new0053 vs. ExPASy Swiss-Prot
Match: Q9C6S1 (Formin-like protein 14 OS=Arabidopsis thaliana OX=3702 GN=FH14 PE=3 SV=3)

HSP 1 Score: 347.8 bits (891), Expect = 1.9e-94
Identity = 213/518 (41.12%), Postives = 310/518 (59.85%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           + FNFREG+++S  +  L +YD+TVL+YP  YEGCP+L L +I  FLR  ESWL+   +Q
Sbjct: 61  LAFNFREGEKKSVFAETLCEYDVTVLEYPRQYEGCPMLPLSLIQHFLRVCESWLARGNRQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           +V+L+HCERGGWP+LAF+LA  L++RK +SGE++TLE+V+++APK LL LLSPLNP PS 
Sbjct: 121 DVILLHCERGGWPLLAFILASFLIFRKVHSGERRTLEIVHREAPKGLLQLLSPLNPFPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQY++RRN++S+WPP +  L LDC+IIR IP FD   GCR ++ ++G++ S+ +  +
Sbjct: 181 LRYLQYVARRNINSEWPPPERALSLDCVIIRGIPNFDSQHGCRPIIRIFGRNYSSKSGLS 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            ++++S    KK +R Y QAEC ++K+DI+C VQG+VV+EC+H+D D   E M+FR MF+
Sbjct: 241 TEMVYSMSDKKKPLRHYRQAECDVIKIDIQCWVQGDVVLECVHMDLDPEREVMMFRVMFN 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVP-NLATALDDEDEYETG 300
           TAF+RSNI+ML+ D +D+ W+A+D + K FR E LF + +   P  + T + + D  ETG
Sbjct: 301 TAFIRSNILMLNSDNLDILWEAKDHYPKGFRAEVLFGEVENASPQKVPTPIVNGD--ETG 360

Query: 301 AASFEEFIEVEEIFNNV---MDGQEA----------------------KGS---NDPPAF 360
               E F  V+E+F+ V    +G +A                      KGS   N P + 
Sbjct: 361 GLPIEAFSRVQELFSGVDLAENGDDAALWLLKQLAAINDAKEFTRFRHKGSFYFNSPDSE 420

Query: 361 QD------CASADGNFKHDKKS------DFDSVKDITV------DDVKYKLDENIYSELN 420
           ++        S+D  F+  ++       D D   DIT+       +  ++   + + E+ 
Sbjct: 421 EETNTSSAADSSDEGFEAIQRPRIHIPFDNDDTDDITLSVAHESSEEPHEFSHHHHHEIP 480

Query: 421 AVKNTV---VEEDMDPPFSRQ---------PPPWLQIYSSVSTVMVAGSIPPPPPPPPPM 451
           A K++V   +    DPP S           PPP   +++  ST   + S PPPPPPPPP+
Sbjct: 481 A-KDSVDNPLNLPSDPPSSGDHVTLLPPPPPPPPPPLFT--STTSFSPSQPPPPPPPPPL 540

BLAST of MC06g_new0053 vs. NCBI nr
Match: XP_022134848.1 (formin-like protein 20 isoform X2 [Momordica charantia] >XP_022134849.1 formin-like protein 20 isoform X2 [Momordica charantia])

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 60
           MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV
Sbjct: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 60

Query: 61  VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 120
           VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR
Sbjct: 61  VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 120

Query: 121 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 180
           YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK
Sbjct: 121 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 180

Query: 181 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 240
           LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA
Sbjct: 181 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 240

Query: 241 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 300
           FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS
Sbjct: 241 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 300

Query: 301 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 360
           FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY
Sbjct: 301 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 360

Query: 361 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 420
           KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV
Sbjct: 361 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 420

Query: 421 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 473
           HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL
Sbjct: 421 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 473

BLAST of MC06g_new0053 vs. NCBI nr
Match: XP_022134847.1 (formin-like protein 20 isoform X1 [Momordica charantia])

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 60
           MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV
Sbjct: 63  MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 122

Query: 61  VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 120
           VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR
Sbjct: 123 VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 182

Query: 121 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 180
           YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK
Sbjct: 183 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 242

Query: 181 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 240
           LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA
Sbjct: 243 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 302

Query: 241 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 300
           FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS
Sbjct: 303 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 362

Query: 301 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 360
           FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY
Sbjct: 363 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 422

Query: 361 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 420
           KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV
Sbjct: 423 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 482

Query: 421 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 473
           HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL
Sbjct: 483 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 535

BLAST of MC06g_new0053 vs. NCBI nr
Match: XP_031745380.1 (formin-like protein 20 [Cucumis sativus])

HSP 1 Score: 643 bits (1658), Expect = 9.73e-223
Identity = 323/400 (80.75%), Postives = 349/400 (87.25%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREG R+SQIS++LTQYDMTV+DYP  YEGCPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGGRRSQISDVLTQYDMTVMDYPRQYEGCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLS LNPQPS 
Sbjct: 121 NVLLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSSLNPQPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPL+LDCLI+RV+PLFDGGKGCR VV +YGQDPSAPANRT
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLILDCLILRVLPLFDGGKGCRPVVRIYGQDPSAPANRT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            KLLFSTPI +KHIR+YLQAECMLVK+DI C VQG+VV+ECIH+DEDLV EEM+FR MFH
Sbjct: 241 SKLLFSTPIKRKHIRNYLQAECMLVKIDIHCHVQGDVVLECIHMDEDLVHEEMMFRVMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAFVRSNIM+L+ DEVDV WDARDQ+ KDFRVEALFLDADAVVPNL  + DDED  ETGA
Sbjct: 301 TAFVRSNIMILNRDEVDVLWDARDQYPKDFRVEALFLDADAVVPNLTASFDDEDGNETGA 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEAKGSNDP------------------PAFQDCASADGNFK 360
           AS EEF EVEEIF+NVMDGQEAKGSNDP                  PAFQDCAS DGN K
Sbjct: 361 ASPEEFFEVEEIFSNVMDGQEAKGSNDPHVVNHVNRKEEWKEDFDPPAFQDCASDDGNLK 420

Query: 361 HDKKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 380
           H KKSDFD+VKDITVDDVKYKLDENIYSELNAVK+  V++
Sbjct: 421 HYKKSDFDAVKDITVDDVKYKLDENIYSELNAVKDIAVDD 460

BLAST of MC06g_new0053 vs. NCBI nr
Match: XP_022134758.1 (formin-like protein 20 [Momordica charantia])

HSP 1 Score: 653 bits (1684), Expect = 8.59e-222
Identity = 328/400 (82.00%), Postives = 350/400 (87.50%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREGDR+SQ+S+ILTQYDMTV+DYP  YEGCPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGDRRSQVSDILTQYDMTVMDYPRQYEGCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH
Sbjct: 121 NVLLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPLVLDC+I+RV+PLFDGGKGCR VV VYGQDPS PANRT
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLVLDCIILRVLPLFDGGKGCRPVVRVYGQDPSTPANRT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            KLLFSTP+ KKHIR+YLQAECMLVK+DI CRVQG+VV+ECIHLDEDLV EEMVFR MFH
Sbjct: 241 SKLLFSTPVKKKHIRNYLQAECMLVKIDIHCRVQGDVVLECIHLDEDLVHEEMVFRVMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           T FVRSNIM+LS DEVDV WDARDQF KDFRVEALFLDADAVV +L TA DDED  ETGA
Sbjct: 301 TTFVRSNIMILSRDEVDVLWDARDQFPKDFRVEALFLDADAVVHDLTTAFDDEDANETGA 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEAKGSNDP------------------PAFQDCASADGNFK 360
           AS EEF EVEEIF+NVMDGQE KGSNDP                  PAFQDCAS DGN K
Sbjct: 361 ASPEEFFEVEEIFSNVMDGQEVKGSNDPQVVNRVDRKEEWKEDMDPPAFQDCASDDGNLK 420

Query: 361 HDKKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 380
           HDKKSDFD+VKDITVDDVKYKLDENIYS+LN VK+  V++
Sbjct: 421 HDKKSDFDAVKDITVDDVKYKLDENIYSDLNTVKDIAVDD 460

BLAST of MC06g_new0053 vs. NCBI nr
Match: KAA0057964.1 (formin-like protein 20 [Cucumis melo var. makuwa])

HSP 1 Score: 644 bits (1660), Expect = 9.41e-217
Identity = 322/400 (80.50%), Postives = 349/400 (87.25%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREG R+SQIS++LTQYDMTV+DYP  YEGCPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGGRRSQISDVLTQYDMTVMDYPRQYEGCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPS 
Sbjct: 121 NVLLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPL+LDCLI+RV+PLFDGGKGCR VV +YGQDPSAPANRT
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLILDCLILRVLPLFDGGKGCRPVVRIYGQDPSAPANRT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            KLLFSTPI +KHIR+YLQAECMLVK+DI C VQG+VV+ECIH+DEDLV EEM+FR MFH
Sbjct: 241 SKLLFSTPIKRKHIRNYLQAECMLVKIDIHCHVQGDVVLECIHMDEDLVHEEMMFRVMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAFVRSNI+ML+ DEVDV WDARDQ+ KDFRVEALFLDADAVVPNL    DDED  ETGA
Sbjct: 301 TAFVRSNIVMLNRDEVDVLWDARDQYPKDFRVEALFLDADAVVPNLTAPFDDEDGNETGA 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEAKGSNDP------------------PAFQDCASADGNFK 360
           AS EEF EVEEIF+NVMDGQEAKGSNDP                  PAFQDCAS DGN K
Sbjct: 361 ASPEEFFEVEEIFSNVMDGQEAKGSNDPHVVNHVNRKEEWKEDFDPPAFQDCASDDGNLK 420

Query: 361 HDKKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 380
           HDKKSDFD+VKDITVDDVKYKL+ENIY +LNAVK+  V++
Sbjct: 421 HDKKSDFDAVKDITVDDVKYKLNENIYPDLNAVKDIAVDD 460

BLAST of MC06g_new0053 vs. ExPASy TrEMBL
Match: A0A6J1C361 (formin-like protein 20 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007020 PE=4 SV=1)

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 60
           MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV
Sbjct: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 60

Query: 61  VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 120
           VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR
Sbjct: 61  VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 120

Query: 121 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 180
           YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK
Sbjct: 121 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 180

Query: 181 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 240
           LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA
Sbjct: 181 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 240

Query: 241 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 300
           FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS
Sbjct: 241 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 300

Query: 301 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 360
           FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY
Sbjct: 301 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 360

Query: 361 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 420
           KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV
Sbjct: 361 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 420

Query: 421 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 473
           HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL
Sbjct: 421 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 473

BLAST of MC06g_new0053 vs. ExPASy TrEMBL
Match: A0A6J1C0S7 (formin-like protein 20 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007020 PE=4 SV=1)

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 473/473 (100.00%), Postives = 473/473 (100.00%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 60
           MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV
Sbjct: 63  MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMIFLRSSESWLSLDGQQNV 122

Query: 61  VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 120
           VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR
Sbjct: 123 VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 182

Query: 121 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 180
           YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK
Sbjct: 183 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 242

Query: 181 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 240
           LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA
Sbjct: 243 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 302

Query: 241 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 300
           FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS
Sbjct: 303 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 362

Query: 301 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 360
           FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY
Sbjct: 363 FEEFIEVEEIFNNVMDGQEAKGSNDPPAFQDCASADGNFKHDKKSDFDSVKDITVDDVKY 422

Query: 361 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 420
           KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV
Sbjct: 423 KLDENIYSELNAVKNTVVEEDMDPPFSRQPPPWLQIYSSVSTVMVAGSIPPPPPPPPPMV 482

Query: 421 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 473
           HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL
Sbjct: 483 HKAFPSPSLVRGTPPPPPSKHGVPPPPPPPKECLYFGQLKHQKRTSISQKYKL 535

BLAST of MC06g_new0053 vs. ExPASy TrEMBL
Match: A0A6J1C2X6 (Formin-like protein OS=Momordica charantia OX=3673 GN=LOC111006954 PE=3 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 4.16e-222
Identity = 328/400 (82.00%), Postives = 350/400 (87.50%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREGDR+SQ+S+ILTQYDMTV+DYP  YEGCPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGDRRSQVSDILTQYDMTVMDYPRQYEGCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH
Sbjct: 121 NVLLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPLVLDC+I+RV+PLFDGGKGCR VV VYGQDPS PANRT
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLVLDCIILRVLPLFDGGKGCRPVVRVYGQDPSTPANRT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            KLLFSTP+ KKHIR+YLQAECMLVK+DI CRVQG+VV+ECIHLDEDLV EEMVFR MFH
Sbjct: 241 SKLLFSTPVKKKHIRNYLQAECMLVKIDIHCRVQGDVVLECIHLDEDLVHEEMVFRVMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           T FVRSNIM+LS DEVDV WDARDQF KDFRVEALFLDADAVV +L TA DDED  ETGA
Sbjct: 301 TTFVRSNIMILSRDEVDVLWDARDQFPKDFRVEALFLDADAVVHDLTTAFDDEDANETGA 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEAKGSNDP------------------PAFQDCASADGNFK 360
           AS EEF EVEEIF+NVMDGQE KGSNDP                  PAFQDCAS DGN K
Sbjct: 361 ASPEEFFEVEEIFSNVMDGQEVKGSNDPQVVNRVDRKEEWKEDMDPPAFQDCASDDGNLK 420

Query: 361 HDKKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 380
           HDKKSDFD+VKDITVDDVKYKLDENIYS+LN VK+  V++
Sbjct: 421 HDKKSDFDAVKDITVDDVKYKLDENIYSDLNTVKDIAVDD 460

BLAST of MC06g_new0053 vs. ExPASy TrEMBL
Match: A0A5A7US19 (Formin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002670 PE=3 SV=1)

HSP 1 Score: 644 bits (1660), Expect = 4.56e-217
Identity = 322/400 (80.50%), Postives = 349/400 (87.25%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREG R+SQIS++LTQYDMTV+DYP  YEGCPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGGRRSQISDVLTQYDMTVMDYPRQYEGCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPS 
Sbjct: 121 NVLLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPL+LDCLI+RV+PLFDGGKGCR VV +YGQDPSAPANRT
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLILDCLILRVLPLFDGGKGCRPVVRIYGQDPSAPANRT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            KLLFSTPI +KHIR+YLQAECMLVK+DI C VQG+VV+ECIH+DEDLV EEM+FR MFH
Sbjct: 241 SKLLFSTPIKRKHIRNYLQAECMLVKIDIHCHVQGDVVLECIHMDEDLVHEEMMFRVMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAFVRSNI+ML+ DEVDV WDARDQ+ KDFRVEALFLDADAVVPNL    DDED  ETGA
Sbjct: 301 TAFVRSNIVMLNRDEVDVLWDARDQYPKDFRVEALFLDADAVVPNLTAPFDDEDGNETGA 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEAKGSNDP------------------PAFQDCASADGNFK 360
           AS EEF EVEEIF+NVMDGQEAKGSNDP                  PAFQDCAS DGN K
Sbjct: 361 ASPEEFFEVEEIFSNVMDGQEAKGSNDPHVVNHVNRKEEWKEDFDPPAFQDCASDDGNLK 420

Query: 361 HDKKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 380
           HDKKSDFD+VKDITVDDVKYKL+ENIY +LNAVK+  V++
Sbjct: 421 HDKKSDFDAVKDITVDDVKYKLNENIYPDLNAVKDIAVDD 460

BLAST of MC06g_new0053 vs. ExPASy TrEMBL
Match: A0A1S3BWY1 (Formin-like protein OS=Cucumis melo OX=3656 GN=LOC103494331 PE=3 SV=1)

HSP 1 Score: 644 bits (1660), Expect = 4.87e-216
Identity = 322/400 (80.50%), Postives = 349/400 (87.25%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREG R+SQIS++LTQYDMTV+DYP  YEGCPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGGRRSQISDVLTQYDMTVMDYPRQYEGCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPS 
Sbjct: 121 NVLLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPL+LDCLI+RV+PLFDGGKGCR VV +YGQDPSAPANRT
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLILDCLILRVLPLFDGGKGCRPVVRIYGQDPSAPANRT 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            KLLFSTPI +KHIR+YLQAECMLVK+DI C VQG+VV+ECIH+DEDLV EEM+FR MFH
Sbjct: 241 SKLLFSTPIKRKHIRNYLQAECMLVKIDIHCHVQGDVVLECIHMDEDLVHEEMMFRVMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAFVRSNI+ML+ DEVDV WDARDQ+ KDFRVEALFLDADAVVPNL    DDED  ETGA
Sbjct: 301 TAFVRSNIVMLNRDEVDVLWDARDQYPKDFRVEALFLDADAVVPNLTAPFDDEDGNETGA 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEAKGSNDP------------------PAFQDCASADGNFK 360
           AS EEF EVEEIF+NVMDGQEAKGSNDP                  PAFQDCAS DGN K
Sbjct: 361 ASPEEFFEVEEIFSNVMDGQEAKGSNDPHVVNHVNRKEEWKEDFDPPAFQDCASDDGNLK 420

Query: 361 HDKKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 380
           HDKKSDFD+VKDITVDDVKYKL+ENIY +LNAVK+  V++
Sbjct: 421 HDKKSDFDAVKDITVDDVKYKLNENIYPDLNAVKDIAVDD 460

BLAST of MC06g_new0053 vs. TAIR 10
Match: AT5G07740.1 (actin binding )

HSP 1 Score: 510.8 bits (1314), Expect = 1.2e-144
Identity = 259/407 (63.64%), Postives = 310/407 (76.17%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFREG+++SQIS++L+QYDMTV+DYP  YE CPLL LEMI  FLRSSESWLSL+GQQ
Sbjct: 61  MVFNFREGEQRSQISDVLSQYDMTVMDYPRQYESCPLLPLEMIHHFLRSSESWLSLEGQQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           NV+LMHCERGGWPVLAFML+GLLLYRKQY GEQKTLEMV+KQAPKELLHLLSPLNPQPS 
Sbjct: 121 NVLLMHCERGGWPVLAFMLSGLLLYRKQYHGEQKTLEMVHKQAPKELLHLLSPLNPQPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQYISRRNL SDWPPSDTPL+LDCLI+R +P F+G KGCR ++ VYGQDP A  NR+
Sbjct: 181 LRYLQYISRRNLGSDWPPSDTPLLLDCLILRDLPHFEGKKGCRPILRVYGQDPKARTNRS 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
             LLFST   KKH R Y Q EC+LVK+DI+CRVQG+VV+ECIHL +DLV EEMVFR MFH
Sbjct: 241 SILLFSTLKTKKHTRLYQQEECILVKLDIQCRVQGDVVLECIHLHDDLVSEEMVFRIMFH 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAFVR+NI+ML  DE+D+ WD +DQF K+F+ E LF  ADAVVP + T+   +DE +   
Sbjct: 301 TAFVRANILMLQRDEMDILWDVKDQFPKEFKAEVLFSGADAVVPPITTSTLSDDENDFDM 360

Query: 301 ASFEEFIEVEEIFNNVMDGQEA-----------------------KGSNDPPAFQDCASA 360
            S EEF EVEEIF++V+DG +                        KG  +P AF DCAS 
Sbjct: 361 TSPEEFFEVEEIFSDVIDGPDHKRDSDSFVVVDTASDDSEGKEVWKGDVEPNAFLDCASD 420

Query: 361 DGNFKHD--KKSDFDSVKDITVDDVKYKLDENIYSELNAVKNTVVEE 381
           D N KHD   ++  D VKDITVDDV+Y+ D    S +++VK+  +++
Sbjct: 421 DSNHKHDMHAETSTDPVKDITVDDVQYRSDGKADSNIDSVKDIGIDD 467


HSP 2 Score: 37.4 bits (85), Expect = 3.9e-02
Identity = 26/44 (59.09%), Postives = 28/44 (63.64%), Query Frame = 0

Query: 411  PPPPPPPPMVHKA--FPSPSLVRGT--PPPPPSKHGVPPPPPPP 451
            PPPPPPPPM   A   P P +  G   PPPPP + G PPPPPPP
Sbjct: 1083 PPPPPPPPMHGGAPPPPPPPMFGGAQPPPPPPMRGGAPPPPPPP 1126

BLAST of MC06g_new0053 vs. TAIR 10
Match: AT2G25050.1 (Actin-binding FH2 (Formin Homology) protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-100
Identity = 176/314 (56.05%), Postives = 229/314 (72.93%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFR+GD +S++ ++LT+YDMT++DYP HYEGCPLL++E +  FL+S+ESWL L  QQ
Sbjct: 28  MVFNFRDGDSRSRMESVLTEYDMTIMDYPRHYEGCPLLTMETVHHFLKSAESWLLL-SQQ 87

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           N++L HCE GGWP LAFMLA LLLYRKQ+SGE +TLEM+YKQAP+ELL L+SPLNP PS 
Sbjct: 88  NILLSHCELGGWPTLAFMLASLLLYRKQFSGEHRTLEMIYKQAPRELLQLMSPLNPLPSQ 147

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LR+LQYISRRN+ S WPP D  L LDC+ +R+IP FDG  GCR +  +YGQDP   ++RT
Sbjct: 148 LRFLQYISRRNVGSQWPPLDQALTLDCVNLRLIPDFDGEGGCRPIFRIYGQDPFMASDRT 207

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            K+LFS P   K +R Y QA+C LVK+DI C + G+VV+ECI L  DL  EEM+FR +F+
Sbjct: 208 SKVLFSMPKRSKAVRQYKQADCELVKIDINCHILGDVVLECITLGSDLEREEMMFRVVFN 267

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAF+RSNI+ L+  E+DV W+  D+F KDF  E +F +  A       ++D     E   
Sbjct: 268 TAFLRSNILTLNRGEIDVLWNTTDRFPKDFSAEVIFSEMGA--GKKLASVDLPHMEEKDV 327

Query: 301 ASFEEFIEVEEIFN 313
              E F +V+EIF+
Sbjct: 328 LPMEAFAKVQEIFS 338


HSP 2 Score: 33.9 bits (76), Expect = 4.3e-01
Identity = 33/76 (43.42%), Postives = 40/76 (52.63%), Query Frame = 0

Query: 384 PPFSRQPPPWLQIYSSVSTVMVAGSI----PPPPPPPPPM-VHKAFPSPSLVRGTPPPPP 443
           PP    PPP   + S+ S    + SI    PPPPPPPPP+  H++  S S +   PP PP
Sbjct: 571 PPPPPPPPPISSLRSTPSPSSTSNSIATQGPPPPPPPPPLQSHRSALSSSPL--PPPLPP 630

Query: 444 SK----HGVPPPPPPP 451
            K       PPPPPPP
Sbjct: 631 KKLLATTNPPPPPPPP 644

BLAST of MC06g_new0053 vs. TAIR 10
Match: AT2G25050.2 (Actin-binding FH2 (Formin Homology) protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-100
Identity = 176/314 (56.05%), Postives = 229/314 (72.93%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           M FNFR+GD +S++ ++LT+YDMT++DYP HYEGCPLL++E +  FL+S+ESWL L  QQ
Sbjct: 28  MVFNFRDGDSRSRMESVLTEYDMTIMDYPRHYEGCPLLTMETVHHFLKSAESWLLL-SQQ 87

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           N++L HCE GGWP LAFMLA LLLYRKQ+SGE +TLEM+YKQAP+ELL L+SPLNP PS 
Sbjct: 88  NILLSHCELGGWPTLAFMLASLLLYRKQFSGEHRTLEMIYKQAPRELLQLMSPLNPLPSQ 147

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LR+LQYISRRN+ S WPP D  L LDC+ +R+IP FDG  GCR +  +YGQDP   ++RT
Sbjct: 148 LRFLQYISRRNVGSQWPPLDQALTLDCVNLRLIPDFDGEGGCRPIFRIYGQDPFMASDRT 207

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            K+LFS P   K +R Y QA+C LVK+DI C + G+VV+ECI L  DL  EEM+FR +F+
Sbjct: 208 SKVLFSMPKRSKAVRQYKQADCELVKIDINCHILGDVVLECITLGSDLEREEMMFRVVFN 267

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGA 300
           TAF+RSNI+ L+  E+DV W+  D+F KDF  E +F +  A       ++D     E   
Sbjct: 268 TAFLRSNILTLNRGEIDVLWNTTDRFPKDFSAEVIFSEMGA--GKKLASVDLPHMEEKDV 327

Query: 301 ASFEEFIEVEEIFN 313
              E F +V+EIF+
Sbjct: 328 LPMEAFAKVQEIFS 338


HSP 2 Score: 33.9 bits (76), Expect = 4.3e-01
Identity = 33/76 (43.42%), Postives = 40/76 (52.63%), Query Frame = 0

Query: 384 PPFSRQPPPWLQIYSSVSTVMVAGSI----PPPPPPPPPM-VHKAFPSPSLVRGTPPPPP 443
           PP    PPP   + S+ S    + SI    PPPPPPPPP+  H++  S S +   PP PP
Sbjct: 571 PPPPPPPPPISSLRSTPSPSSTSNSIATQGPPPPPPPPPLQSHRSALSSSPL--PPPLPP 630

Query: 444 SK----HGVPPPPPPP 451
            K       PPPPPPP
Sbjct: 631 KKLLATTNPPPPPPPP 644

BLAST of MC06g_new0053 vs. TAIR 10
Match: AT1G31810.1 (Formin Homology 14 )

HSP 1 Score: 347.8 bits (891), Expect = 1.4e-95
Identity = 213/518 (41.12%), Postives = 310/518 (59.85%), Query Frame = 0

Query: 1   MGFNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQ 60
           + FNFREG+++S  +  L +YD+TVL+YP  YEGCP+L L +I  FLR  ESWL+   +Q
Sbjct: 61  LAFNFREGEKKSVFAETLCEYDVTVLEYPRQYEGCPMLPLSLIQHFLRVCESWLARGNRQ 120

Query: 61  NVVLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSH 120
           +V+L+HCERGGWP+LAF+LA  L++RK +SGE++TLE+V+++APK LL LLSPLNP PS 
Sbjct: 121 DVILLHCERGGWPLLAFILASFLIFRKVHSGERRTLEIVHREAPKGLLQLLSPLNPFPSQ 180

Query: 121 LRYLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRT 180
           LRYLQY++RRN++S+WPP +  L LDC+IIR IP FD   GCR ++ ++G++ S+ +  +
Sbjct: 181 LRYLQYVARRNINSEWPPPERALSLDCVIIRGIPNFDSQHGCRPIIRIFGRNYSSKSGLS 240

Query: 181 LKLLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFH 240
            ++++S    KK +R Y QAEC ++K+DI+C VQG+VV+EC+H+D D   E M+FR MF+
Sbjct: 241 TEMVYSMSDKKKPLRHYRQAECDVIKIDIQCWVQGDVVLECVHMDLDPEREVMMFRVMFN 300

Query: 241 TAFVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVP-NLATALDDEDEYETG 300
           TAF+RSNI+ML+ D +D+ W+A+D + K FR E LF + +   P  + T + + D  ETG
Sbjct: 301 TAFIRSNILMLNSDNLDILWEAKDHYPKGFRAEVLFGEVENASPQKVPTPIVNGD--ETG 360

Query: 301 AASFEEFIEVEEIFNNV---MDGQEA----------------------KGS---NDPPAF 360
               E F  V+E+F+ V    +G +A                      KGS   N P + 
Sbjct: 361 GLPIEAFSRVQELFSGVDLAENGDDAALWLLKQLAAINDAKEFTRFRHKGSFYFNSPDSE 420

Query: 361 QD------CASADGNFKHDKKS------DFDSVKDITV------DDVKYKLDENIYSELN 420
           ++        S+D  F+  ++       D D   DIT+       +  ++   + + E+ 
Sbjct: 421 EETNTSSAADSSDEGFEAIQRPRIHIPFDNDDTDDITLSVAHESSEEPHEFSHHHHHEIP 480

Query: 421 AVKNTV---VEEDMDPPFSRQ---------PPPWLQIYSSVSTVMVAGSIPPPPPPPPPM 451
           A K++V   +    DPP S           PPP   +++  ST   + S PPPPPPPPP+
Sbjct: 481 A-KDSVDNPLNLPSDPPSSGDHVTLLPPPPPPPPPPLFT--STTSFSPSQPPPPPPPPPL 540

BLAST of MC06g_new0053 vs. TAIR 10
Match: AT5G58160.1 (actin binding )

HSP 1 Score: 331.6 bits (849), Expect = 1.0e-90
Identity = 214/496 (43.15%), Postives = 281/496 (56.65%), Query Frame = 0

Query: 3   FNFREGDRQSQISNILTQYDMTVLDYPGHYEGCPLLSLEMI--FLRSSESWLSLDGQQNV 62
           FNFRE   +S ++++L+++ +T++DYP HYEGC LL +E++  FLRSSESWLSL G  N+
Sbjct: 63  FNFREVGTRSVMADVLSEHGLTIMDYPRHYEGCSLLPVEVMHHFLRSSESWLSL-GPNNL 122

Query: 63  VLMHCERGGWPVLAFMLAGLLLYRKQYSGEQKTLEMVYKQAPKELLHLLSPLNPQPSHLR 122
           +LMHCE G WPVLAFMLA LL+YRKQYSGE KTL+M+YKQAP+ELL L SPLNP PS LR
Sbjct: 123 LLMHCESGAWPVLAFMLAALLIYRKQYSGESKTLDMIYKQAPRELLRLFSPLNPIPSQLR 182

Query: 123 YLQYISRRNLDSDWPPSDTPLVLDCLIIRVIPLFDGGKGCRHVVHVYGQDPSAPANRTLK 182
           YLQY+SRRNL S+WPP D  L +DC+I+R IP   G  G R +  +YGQDP    ++  K
Sbjct: 183 YLQYVSRRNLVSEWPPLDRALTMDCVILRFIPDVSGQGGFRPMFRIYGQDPFFVDDKKPK 242

Query: 183 LLFSTPINKKHIRSYLQAECMLVKVDIRCRVQGNVVVECIHLDEDLVGEEMVFRFMFHTA 242
           LL++TP   KH+R Y QAEC LVK+DI C VQG++V+EC+ L++D+  E M+FR +F+TA
Sbjct: 243 LLYTTPKKGKHLRVYKQAECELVKIDINCHVQGDIVIECLSLNDDMEREVMMFRVVFNTA 302

Query: 243 FVRSNIMMLSCDEVDVAWDARDQFSKDFRVEALFLDADAVVPNLATALDDEDEYETGAAS 302
           F+RSNI+ML+ DEVD  W  + +F K FRVE LF D DA   +    ++     E     
Sbjct: 303 FIRSNILMLNRDEVDTLWHIK-EFPKGFRVELLFSDMDAA--SSVDLMNFSSLEEKDGLP 362

Query: 303 FEEFIEVEEIFNNV-----MDG--------------QEAKGSNDPPAFQDCA-------- 362
            E F +V E FN V      D               QE    N  P  Q  +        
Sbjct: 363 IEVFSKVHEFFNQVDWVDQTDATRNMFQQLAIANAVQEGLDGNSSPRLQGLSPKSIHDIM 422

Query: 363 --SADGNFKHDKKSDFDSVKDI------TVDDVKYKLDENIYSELNAVKNTVVEEDMDPP 422
             +A  N    K S    V+ I        D VK  + E+++S L          +    
Sbjct: 423 KHAAIENSAKFKLSSMSEVETIDTPEKPPTDSVKKFIAEDVHSVLQINNQEQNASEDATK 482

Query: 423 FSRQPPPWLQIYSSVSTV--MVAGSIPPPPP----PPPPMVHKAFPSPSLVRGTPPPPP- 450
              Q  P L++    +TV  +V  S  P       P  P  H    + S    TP PP  
Sbjct: 483 LLHQESPSLKLVHHSATVKPLVDDSKSPENAEENFPKSPSAHDG-KAISFSPPTPSPPHP 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLQ71.7e-14363.64Formin-like protein 20 OS=Arabidopsis thaliana OX=3702 GN=FH20 PE=2 SV=3[more]
Q6ZCX33.1e-10558.23Formin-like protein 6 OS=Oryza sativa subsp. japonica OX=39947 GN=FH6 PE=2 SV=2[more]
Q9SK282.0e-9956.05Formin-like protein 18 OS=Arabidopsis thaliana OX=3702 GN=FH18 PE=2 SV=3[more]
Q84ZL02.2e-9549.59Formin-like protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=FH5 PE=2 SV=2[more]
Q9C6S11.9e-9441.12Formin-like protein 14 OS=Arabidopsis thaliana OX=3702 GN=FH14 PE=3 SV=3[more]
Match NameE-valueIdentityDescription
XP_022134848.10.0100.00formin-like protein 20 isoform X2 [Momordica charantia] >XP_022134849.1 formin-l... [more]
XP_022134847.10.0100.00formin-like protein 20 isoform X1 [Momordica charantia][more]
XP_031745380.19.73e-22380.75formin-like protein 20 [Cucumis sativus][more]
XP_022134758.18.59e-22282.00formin-like protein 20 [Momordica charantia][more]
KAA0057964.19.41e-21780.50formin-like protein 20 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1C3610.0100.00formin-like protein 20 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007020... [more]
A0A6J1C0S70.0100.00formin-like protein 20 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007020... [more]
A0A6J1C2X64.16e-22282.00Formin-like protein OS=Momordica charantia OX=3673 GN=LOC111006954 PE=3 SV=1[more]
A0A5A7US194.56e-21780.50Formin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G... [more]
A0A1S3BWY14.87e-21680.50Formin-like protein OS=Cucumis melo OX=3656 GN=LOC103494331 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07740.11.2e-14463.64actin binding [more]
AT2G25050.11.4e-10056.05Actin-binding FH2 (Formin Homology) protein [more]
AT2G25050.21.4e-10056.05Actin-binding FH2 (Formin Homology) protein [more]
AT1G31810.11.4e-9541.12Formin Homology 14 [more]
AT5G58160.11.0e-9043.15actin binding [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014020Tensin phosphatase, C2 domainSMARTSM01326PTEN_C2_2coord: 136..276
e-value: 1.1E-39
score: 147.8
IPR014020Tensin phosphatase, C2 domainPFAMPF10409PTEN_C2coord: 138..274
e-value: 1.0E-24
score: 86.8
IPR014020Tensin phosphatase, C2 domainPROSITEPS51182C2_TENSINcoord: 138..277
score: 28.081102
NoneNo IPR availableGENE3D2.60.40.1110coord: 140..279
e-value: 3.8E-34
score: 119.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 413..454
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 413..453
NoneNo IPR availablePANTHERPTHR45733:SF10FORMIN-LIKE PROTEIN 15A-RELATEDcoord: 1..450
NoneNo IPR availablePANTHERPTHR45733FORMIN-Jcoord: 1..450
IPR029021Protein-tyrosine phosphatase-likeGENE3D3.90.190.10Protein tyrosine phosphatase superfamilycoord: 1..139
e-value: 1.5E-19
score: 72.6
IPR029021Protein-tyrosine phosphatase-likeSUPERFAMILY52799(Phosphotyrosine protein) phosphatases IIcoord: 16..126
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 139..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0053.1MC06g_new0053.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0030150 protein import into mitochondrial matrix
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005744 TIM23 mitochondrial import inner membrane translocase complex
molecular_function GO:0004721 phosphoprotein phosphatase activity
molecular_function GO:0005249 voltage-gated potassium channel activity