MC06g_new0049 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g_new0049
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionpentatricopeptide repeat-containing protein At5g61370, mitochondrial
LocationMC06: 1646884 .. 1648365 (+)
RNA-Seq ExpressionMC06g_new0049
SyntenyMC06g_new0049
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGCACGCCATATGTTCGATGAAATTCCGCTGAGAAGATGCGTCTCCGCGTTGAAGTTAAAATGGCAGAGCTTCATTACGCAATCTATCAGCACGGACCTTCGCTTCTGCTCCCTTCATTCCACCGTAACTAATGCAGCTTCTGTGGTGGATTTGTGTAAAGTGGTTTCCTGCTCGATCGGTGGCCTAGATGAATTAGAATCCAGTCTGAACAAATGTACAGTATCCTTGACATCTCCACTTGTCACCCAGGTCATTGATTCTTGCAAAAACGAAGCACCCACTAGAAGATTACTCAGGTTTTTCCTGTGGTCTCTCAAGAATTTGAAACATGATTTGGAAGATGAAGATTTCAATCATGCCATCCGCTTATTTGCTCAGAAGAAGGACTACAACGCCATTGATATTTTACTTTCCGATCTTAAGAAAGCTGGCCGGGCAATGGAGGGCGAGACCTTCGGTCTTGTGGCTGAGGCTTTAGTGAAACTGGATAGGGAAGATGAAGCACTGGGCCTTTTCAAGAACTTGGACAAGTACAAGTGCCCCCAAGACCAGTTCACTGTCACTGCAATTGTTACTGCTCTCTGTGCAAAAGGGCATGCCAAGAGAGCAGAAGGGGTTGTTTTGCACCACAGAGACAAGATCTCTGGCAGCAAGAGTTGCATCTATAGAAGCCTCCTATATGGATGGTATGTTCAGAATAACGCAAAGGAAGCAAGAAGGATACTTAAAGAAATGAAGTCGGATGGAGCCATGCCAGATTTGTTCTGCTACAACACTTTCCTCAGGTGCCTCTGTGAGCAGAATCTTAAGAAGAATCCTTCAGGTCTTGTGCCTGAAGCTTTGAATGTGATGATGGAAATGAGATCCTACAAAATCTCCCCAAACTCAATCAGTTATAATATATTGTTATCTTGTCTTGGTAGGACTAGGAGAGTGAAGGAATCGTGTAGAATCCTCGAGACGATGAAACGATCTGGTTGTCGAGCTGATTGGGTCAGCTATTATCTTGTGGCAAGAGTGTTGTTCCTGACTGGAAGATTTGGGAAAGGGCGCAGGATTGTGGATGAGATGATTGACGAGGGATTGATCCCGGATCAAAAATTTTACTACGATTTGATTGGTATTCTATGTGGAGTTGAGAGAGTAAACTATGCGCTTGAGATGTTGGATAAAATGAAGAGAAGCTCATTGGGGGGTTATGGTCCAGTTTATGATGTGCTAATACCGAAGTTGTGCAGAGGAGGTGATTTTGAAATGGGCAGGCAACTGTGGGAGGAGGCCATGGCTATGGGTCTGAAGCTTCGCTGCTCAAGGGAGGTTTTGGATCCTTCAATCACGAAGGTTTGGCTTCCAACCAGAAAGACTGACAGCAAAATTCTTGAAGAAGTCAATGGTGCTGAGAAGCAGAACCAAGCTGCTGCAACTGACCGACCAAAAGCTAAGGGAAAGAAAAGTAAGAATCTGTCTTTGAAATAA

mRNA sequence

ATGAATGCACGCCATATGTTCGATGAAATTCCGCTGAGAAGATGCGTCTCCGCGTTGAAGTTAAAATGGCAGAGCTTCATTACGCAATCTATCAGCACGGACCTTCGCTTCTGCTCCCTTCATTCCACCGTAACTAATGCAGCTTCTGTGGTGGATTTGTGTAAAGTGGTTTCCTGCTCGATCGGTGGCCTAGATGAATTAGAATCCAGTCTGAACAAATGTACAGTATCCTTGACATCTCCACTTGTCACCCAGGTCATTGATTCTTGCAAAAACGAAGCACCCACTAGAAGATTACTCAGGTTTTTCCTGTGGTCTCTCAAGAATTTGAAACATGATTTGGAAGATGAAGATTTCAATCATGCCATCCGCTTATTTGCTCAGAAGAAGGACTACAACGCCATTGATATTTTACTTTCCGATCTTAAGAAAGCTGGCCGGGCAATGGAGGGCGAGACCTTCGGTCTTGTGGCTGAGGCTTTAGTGAAACTGGATAGGGAAGATGAAGCACTGGGCCTTTTCAAGAACTTGGACAAGTACAAGTGCCCCCAAGACCAGTTCACTGTCACTGCAATTGTTACTGCTCTCTGTGCAAAAGGGCATGCCAAGAGAGCAGAAGGGGTTGTTTTGCACCACAGAGACAAGATCTCTGGCAGCAAGAGTTGCATCTATAGAAGCCTCCTATATGGATGGTATGTTCAGAATAACGCAAAGGAAGCAAGAAGGATACTTAAAGAAATGAAGTCGGATGGAGCCATGCCAGATTTGTTCTGCTACAACACTTTCCTCAGGTGCCTCTGTGAGCAGAATCTTAAGAAGAATCCTTCAGGTCTTGTGCCTGAAGCTTTGAATGTGATGATGGAAATGAGATCCTACAAAATCTCCCCAAACTCAATCAGTTATAATATATTGTTATCTTGTCTTGGTAGGACTAGGAGAGTGAAGGAATCGTGTAGAATCCTCGAGACGATGAAACGATCTGGTTGTCGAGCTGATTGGGTCAGCTATTATCTTGTGGCAAGAGTGTTGTTCCTGACTGGAAGATTTGGGAAAGGGCGCAGGATTGTGGATGAGATGATTGACGAGGGATTGATCCCGGATCAAAAATTTTACTACGATTTGATTGGTATTCTATGTGGAGTTGAGAGAGTAAACTATGCGCTTGAGATGTTGGATAAAATGAAGAGAAGCTCATTGGGGGGTTATGGTCCAGTTTATGATGTGCTAATACCGAAGTTGTGCAGAGGAGGTGATTTTGAAATGGGCAGGCAACTGTGGGAGGAGGCCATGGCTATGGGTCTGAAGCTTCGCTGCTCAAGGGAGGTTTTGGATCCTTCAATCACGAAGGTTTGGCTTCCAACCAGAAAGACTGACAGCAAAATTCTTGAAGAAGTCAATGGTGCTGAGAAGCAGAACCAAGCTGCTGCAACTGACCGACCAAAAGCTAAGGGAAAGAAAAGTAAGAATCTGTCTTTGAAATAA

Coding sequence (CDS)

ATGAATGCACGCCATATGTTCGATGAAATTCCGCTGAGAAGATGCGTCTCCGCGTTGAAGTTAAAATGGCAGAGCTTCATTACGCAATCTATCAGCACGGACCTTCGCTTCTGCTCCCTTCATTCCACCGTAACTAATGCAGCTTCTGTGGTGGATTTGTGTAAAGTGGTTTCCTGCTCGATCGGTGGCCTAGATGAATTAGAATCCAGTCTGAACAAATGTACAGTATCCTTGACATCTCCACTTGTCACCCAGGTCATTGATTCTTGCAAAAACGAAGCACCCACTAGAAGATTACTCAGGTTTTTCCTGTGGTCTCTCAAGAATTTGAAACATGATTTGGAAGATGAAGATTTCAATCATGCCATCCGCTTATTTGCTCAGAAGAAGGACTACAACGCCATTGATATTTTACTTTCCGATCTTAAGAAAGCTGGCCGGGCAATGGAGGGCGAGACCTTCGGTCTTGTGGCTGAGGCTTTAGTGAAACTGGATAGGGAAGATGAAGCACTGGGCCTTTTCAAGAACTTGGACAAGTACAAGTGCCCCCAAGACCAGTTCACTGTCACTGCAATTGTTACTGCTCTCTGTGCAAAAGGGCATGCCAAGAGAGCAGAAGGGGTTGTTTTGCACCACAGAGACAAGATCTCTGGCAGCAAGAGTTGCATCTATAGAAGCCTCCTATATGGATGGTATGTTCAGAATAACGCAAAGGAAGCAAGAAGGATACTTAAAGAAATGAAGTCGGATGGAGCCATGCCAGATTTGTTCTGCTACAACACTTTCCTCAGGTGCCTCTGTGAGCAGAATCTTAAGAAGAATCCTTCAGGTCTTGTGCCTGAAGCTTTGAATGTGATGATGGAAATGAGATCCTACAAAATCTCCCCAAACTCAATCAGTTATAATATATTGTTATCTTGTCTTGGTAGGACTAGGAGAGTGAAGGAATCGTGTAGAATCCTCGAGACGATGAAACGATCTGGTTGTCGAGCTGATTGGGTCAGCTATTATCTTGTGGCAAGAGTGTTGTTCCTGACTGGAAGATTTGGGAAAGGGCGCAGGATTGTGGATGAGATGATTGACGAGGGATTGATCCCGGATCAAAAATTTTACTACGATTTGATTGGTATTCTATGTGGAGTTGAGAGAGTAAACTATGCGCTTGAGATGTTGGATAAAATGAAGAGAAGCTCATTGGGGGGTTATGGTCCAGTTTATGATGTGCTAATACCGAAGTTGTGCAGAGGAGGTGATTTTGAAATGGGCAGGCAACTGTGGGAGGAGGCCATGGCTATGGGTCTGAAGCTTCGCTGCTCAAGGGAGGTTTTGGATCCTTCAATCACGAAGGTTTGGCTTCCAACCAGAAAGACTGACAGCAAAATTCTTGAAGAAGTCAATGGTGCTGAGAAGCAGAACCAAGCTGCTGCAACTGACCGACCAAAAGCTAAGGGAAAGAAAAGTAAGAATCTGTCTTTGAAATAA

Protein sequence

MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCSIGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFNHAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKYKCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEARRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSISYNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMIDEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFEMGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRPKAKGKKSKNLSLK
Homology
BLAST of MC06g_new0049 vs. ExPASy Swiss-Prot
Match: Q9FLJ6 (Pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g61370 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 9.3e-161
Identity = 272/482 (56.43%), Postives = 373/482 (77.39%), Query Frame = 0

Query: 17  SALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCSIGGLDELESSLNKCTV 76
           + ++L   +++T +      FCS H    +  ++ ++ ++VS  +GGLD+LE +LN+ +V
Sbjct: 4   TTVRLNRFTYLTSTAKLTRYFCSHHLVDRSETALHEVIRIVSSPVGGLDDLEENLNQVSV 63

Query: 77  SLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFNHAIRLFAQKKDYNAID 136
           S +S LVTQVI+SCKNE   RRLLRFF WS K+L   L D++FN+ +R+ A+KKD+ A+ 
Sbjct: 64  SPSSNLVTQVIESCKNETSPRRLLRFFSWSCKSLGSSLHDKEFNYVLRVLAEKKDHTAMQ 123

Query: 137 ILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKYKCPQDQFTVTAIVTAL 196
           ILLSDL+K  RAM+ +TF +VAE LVK+ +E++A+G+FK LDK+ CPQD FTVTAI++AL
Sbjct: 124 ILLSDLRKENRAMDKQTFSIVAETLVKVGKEEDAIGIFKILDKFSCPQDGFTVTAIISAL 183

Query: 197 CAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEARRILKEMKSDGAMPDL 256
           C++GH KRA GV+ HH+D ISG++  +YRSLL+GW VQ N KEARR++++MKS G  PDL
Sbjct: 184 CSRGHVKRALGVMHHHKDVISGNELSVYRSLLFGWSVQRNVKEARRVIQDMKSAGITPDL 243

Query: 257 FCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSISYNILLSCLGRTRRVKE 316
           FC+N+ L CLCE+N+ +NPSGLVPEALN+M+EMRSYKI P S+SYNILLSCLGRTRRV+E
Sbjct: 244 FCFNSLLTCLCERNVNRNPSGLVPEALNIMLEMRSYKIQPTSMSYNILLSCLGRTRRVRE 303

Query: 317 SCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMIDEGLIPDQKFYYDLIG 376
           SC+ILE MKRSGC  D  SYY V RVL+LTGRFGKG +IVDEMI+ G  P++KFYYDLIG
Sbjct: 304 SCQILEQMKRSGCDPDTGSYYFVVRVLYLTGRFGKGNQIVDEMIERGFRPERKFYYDLIG 363

Query: 377 ILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFEMGRQLWEEAMAMGLKL 436
           +LCGVERVN+AL++ +KMKRSS+GGYG VYD+LIPKLC+GG+FE GR+LWEEA+++ + L
Sbjct: 364 VLCGVERVNFALQLFEKMKRSSVGGYGQVYDLLIPKLCKGGNFEKGRELWEEALSIDVTL 423

Query: 437 RCSREVLDPSITKVWLPTR-KTDSKILE------EVNGAEKQNQAAATDRPKAKGK-KSK 491
            CS  +LDPS+T+V+ P + K ++ +++      +++    + +     +PK + K K K
Sbjct: 424 SCSISLLDPSVTEVFKPMKMKEEAAMVDRRALNLKIHARMNKTKPKLKLKPKRRSKTKKK 483

BLAST of MC06g_new0049 vs. ExPASy Swiss-Prot
Match: Q9FME4 (Pentatricopeptide repeat-containing protein PNM1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PNM1 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.1e-39
Identity = 105/377 (27.85%), Postives = 180/377 (47.75%), Query Frame = 0

Query: 97  RRLLRFFLWSLKNLKHDLEDEDFNHAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGL 156
           R  L F  W   N      DE  +  +  F ++KD+     +L  + K      G+T   
Sbjct: 124 RAALGFNEWLDSNSNFSHTDETVSFFVDYFGRRKDFKG---MLEIISKYKGIAGGKTLES 183

Query: 157 VAEALVKLDREDEALGLFKNLDK-YKCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDK 216
             + LV+  R  +    F+ ++  Y   +D+ ++T +V  LC KGHA  AE +V +  ++
Sbjct: 184 AIDRLVRAGRPKQVTDFFEKMENDYGLKRDKESLTLVVKKLCEKGHASIAEKMVKNTANE 243

Query: 217 ISGSKSCIYRSLLYGWYVQNNAKEARRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNP 276
           I   ++ I   L+ GW +     EA R+  EM   G       YN  L C+C+   KK+P
Sbjct: 244 IFPDEN-ICDLLISGWCIAEKLDEATRLAGEMSRGGFEIGTKAYNMMLDCVCKLCRKKDP 303

Query: 277 SGLVPEALNVMMEMRSYKISPNSISYNILLSCLGRTRRVKESCRILETMKRSGCRADWVS 336
             L PE   V++EM    +  N+ ++N+L++ L + RR +E+  +   M   GC+ D  +
Sbjct: 304 FKLQPEVEKVLLEMEFRGVPRNTETFNVLINNLCKIRRTEEAMTLFGRMGEWGCQPDAET 363

Query: 337 YYLVARVLFLTGRFGKGRRIVDEMIDEGL--IPDQKFYYDLIGILCGVERVNYALEMLDK 396
           Y ++ R L+   R G+G  ++D+M   G   + ++K YY  + ILCG+ER+ +A+ +   
Sbjct: 364 YLVLIRSLYQAARIGEGDEMIDKMKSAGYGELLNKKEYYGFLKILCGIERLEHAMSVFKS 423

Query: 397 MKRSSLGGYGPVYDVLIPKLCRGGDFEMGRQLWEEAMAMGLKLRCSREVLDPSITKVWLP 456
           MK +        YD+L+ K+C          L++EA   G+ +      +DP   K    
Sbjct: 424 MKANGCKPGIKTYDLLMGKMCANNQLTRANGLYKEAAKKGIAVSPKEYRVDPRFMK--KK 483

Query: 457 TRKTDSKILEEVNGAEK 471
           T++ DS + +     EK
Sbjct: 484 TKEVDSNVKKRETLPEK 494

BLAST of MC06g_new0049 vs. ExPASy Swiss-Prot
Match: P0C8A0 (Pentatricopeptide repeat-containing protein At3g49730 OS=Arabidopsis thaliana OX=3702 GN=At3g49730 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 9.4e-36
Identity = 101/390 (25.90%), Postives = 182/390 (46.67%), Query Frame = 0

Query: 47  AASVVDLCKVVSCSIGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWS 106
           A  V  + +++      + +LE +LN+  + L   L+ +V+  C +        RFFLW+
Sbjct: 64  AGEVEKIYRILRNHHSRVPKLELALNESGIDLRPGLIIRVLSRCGDAGNLG--YRFFLWA 123

Query: 107 LKNLKHDLEDEDFNHAIRLFAQKKDYNAIDILLSDLKKAG-RAMEGETFGLVAEALVKLD 166
            K   +    E     + + ++ + + A+  L+ +++K     +E E F ++       +
Sbjct: 124 TKQPGYFHSYEVCKSMVMILSKMRQFGAVWGLIEEMRKTNPELIEPELFVVLMRRFASAN 183

Query: 167 REDEALGLFKNLDKYKCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYR 226
              +A+ +   + KY    D++    ++ ALC  G  K A  V    R+K   +    + 
Sbjct: 184 MVKKAVEVLDEMPKYGLEPDEYVFGCLLDALCKNGSVKEASKVFEDMREKFPPNLR-YFT 243

Query: 227 SLLYGWYVQNNAKEARRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNV 286
           SLLYGW  +    EA+ +L +MK  G  PD+  +   L            +G + +A ++
Sbjct: 244 SLLYGWCREGKLMEAKEVLVQMKEAGLEPDIVVFTNLLSGYAH-------AGKMADAYDL 303

Query: 287 MMEMRSYKISPNSISYNILLSCLGRT-RRVKESCRILETMKRSGCRADWVSYYLVARVLF 346
           M +MR     PN   Y +L+  L RT +R+ E+ R+   M+R GC AD V+Y  +     
Sbjct: 304 MNDMRKRGFEPNVNCYTVLIQALCRTEKRMDEAMRVFVEMERYGCEADIVTYTALISGFC 363

Query: 347 LTGRFGKGRRIVDEMIDEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGP 406
             G   KG  ++D+M  +G++P Q  Y  ++      E+    LE+++KMKR        
Sbjct: 364 KWGMIDKGYSVLDDMRKKGVMPSQVTYMQIMVAHEKKEQFEECLELIEKMKRRGCHPDLL 423

Query: 407 VYDVLIPKLCRGGDFEMGRQLWEEAMAMGL 435
           +Y+V+I   C+ G+ +   +LW E  A GL
Sbjct: 424 IYNVVIRLACKLGEVKEAVRLWNEMEANGL 443

BLAST of MC06g_new0049 vs. ExPASy Swiss-Prot
Match: Q9LUJ4 (Pentatricopeptide repeat-containing protein At3g22670, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g22670 PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 8.2e-24
Identity = 82/355 (23.10%), Postives = 159/355 (44.79%), Query Frame = 0

Query: 65  DELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFNHAIR 124
           +++   L+KC V +T  LV QV+    N     +   FF+W+     +      +N  + 
Sbjct: 117 EDVVKELSKCDVVVTESLVLQVLRRFSN--GWNQAYGFFIWANSQTGYVHSGHTYNAMVD 176

Query: 125 LFAQKKDYNAIDILLSDLKK--AGRAMEGETFGLVAEALVKLDREDEALGLFKNLDK-YK 184
           +  + ++++ +  L++++ K    + +  +T   V   L K  + ++A+  F  ++K Y 
Sbjct: 177 VLGKCRNFDLMWELVNEMNKNEESKLVTLDTMSKVMRRLAKSGKYNKAVDAFLEMEKSYG 236

Query: 185 CPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEAR 244
              D   + +++ AL  +   + A  V L   D I    +  +  L++G+       +AR
Sbjct: 237 VKTDTIAMNSLMDALVKENSIEHAHEVFLKLFDTIK-PDARTFNILIHGFCKARKFDDAR 296

Query: 245 RILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSISY 304
            ++  MK     PD+  Y +F+   C++       G       ++ EMR    +PN ++Y
Sbjct: 297 AMMDLMKVTEFTPDVVTYTSFVEAYCKE-------GDFRRVNEMLEEMRENGCNPNVVTY 356

Query: 305 NILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMID 364
            I++  LG++++V E+  + E MK  GC  D   Y  +  +L  TGRF     I ++M +
Sbjct: 357 TIVMHSLGKSKQVAEALGVYEKMKEDGCVPDAKFYSSLIHILSKTGRFKDAAEIFEDMTN 416

Query: 365 EGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIP--KLC 415
           +G+  D   Y  +I       R   AL +L +M+        P  +   P  K+C
Sbjct: 417 QGVRRDVLVYNTMISAALHHSRDEMALRLLKRMEDEEGESCSPNVETYAPLLKMC 461

BLAST of MC06g_new0049 vs. ExPASy Swiss-Prot
Match: Q9SSR6 (Pentatricopeptide repeat-containing protein At1g52640, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g52640 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 3.1e-23
Identity = 89/381 (23.36%), Postives = 164/381 (43.04%), Query Frame = 0

Query: 50  VVDLCKVVSCSIGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWS--L 109
           V ++ +V+S      D+LE +L   +  ++S LV QV+  CKN        RFFLW+  +
Sbjct: 38  VNEISRVLSDHRNPKDDLEHTLVAYSPRVSSNLVEQVLKRCKNLGFPAH--RFFLWARRI 97

Query: 110 KNLKHDLEDEDFNHAIRLFAQKKDYNAI-DILLSDLKKAGRAMEGETFGLVAEALVKLDR 169
            +  H L  E ++  + +    K +  + D L+   +     +  + F +V  A  + + 
Sbjct: 98  PDFAHSL--ESYHILVEILGSSKQFALLWDFLIEAREYNYFEISSKVFWIVFRAYSRANL 157

Query: 170 EDEALGLFKNLDKYKCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRS 229
             EA   F  + ++        +  ++ +LC K H   A+      +       +  Y  
Sbjct: 158 PSEACRAFNRMVEFGIKPCVDDLDQLLHSLCDKKHVNHAQEFFGKAKGFGIVPSAKTYSI 217

Query: 230 LLYGWYVQNNAKEARRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVM 289
           L+ GW    +A  AR++  EM     + DL  YN  L  LC+       SG V     + 
Sbjct: 218 LVRGWARIRDASGARKVFDEMLERNCVVDLLAYNALLDALCK-------SGDVDGGYKMF 277

Query: 290 MEMRSYKISPNSISYNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLT 349
            EM +  + P++ S+ I +        V  + ++L+ MKR     +  ++  + + L   
Sbjct: 278 QEMGNLGLKPDAYSFAIFIHAYCDAGDVHSAYKVLDRMKRYDLVPNVYTFNHIIKTLCKN 337

Query: 350 GRFGKGRRIVDEMIDEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVY 409
            +      ++DEMI +G  PD   Y  ++   C    VN A ++L +M R+        Y
Sbjct: 338 EKVDDAYLLLDEMIQKGANPDTWTYNSIMAYHCDHCEVNRATKLLSRMDRTKCLPDRHTY 397

Query: 410 DVLIPKLCRGGDFEMGRQLWE 428
           ++++  L R G F+   ++WE
Sbjct: 398 NMVLKLLIRIGRFDRATEIWE 407

BLAST of MC06g_new0049 vs. NCBI nr
Match: XP_022134610.1 (pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Momordica charantia])

HSP 1 Score: 991 bits (2562), Expect = 0.0
Identity = 492/493 (99.80%), Postives = 492/493 (99.80%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS
Sbjct: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN
Sbjct: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY
Sbjct: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA
Sbjct: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS
Sbjct: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI
Sbjct: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           DEGLIPD KFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE
Sbjct: 361 DEGLIPDXKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP
Sbjct: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480

Query: 481 KAKGKKSKNLSLK 493
           KAKGKKSKNLSLK
Sbjct: 481 KAKGKKSKNLSLK 493

BLAST of MC06g_new0049 vs. NCBI nr
Match: XP_022987424.1 (pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Cucurbita maxima])

HSP 1 Score: 835 bits (2157), Expect = 8.92e-303
Identity = 413/491 (84.11%), Postives = 445/491 (90.63%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           M+ARHMFDE+ LRRCVS LKLKW SF  QS+ T  RFCSLHSTV N ASV  LC+VVSC+
Sbjct: 8   MHARHMFDEMSLRRCVSLLKLKWDSFFVQSVCTQHRFCSLHSTVKNGASVSKLCEVVSCT 67

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELE++LNKCTVSLTS LVTQVIDSCKNEAP RRLLRFF WSLK L H LEDEDFN
Sbjct: 68  IGGLDELEATLNKCTVSLTSSLVTQVIDSCKNEAPNRRLLRFFSWSLKKLNHHLEDEDFN 127

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIRLFAQKKDY AIDILLSDLKKA RAM+G+TFGLVAEALVK+DREDEALGLFKNLDKY
Sbjct: 128 YAIRLFAQKKDYTAIDILLSDLKKARRAMDGQTFGLVAEALVKMDREDEALGLFKNLDKY 187

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTVTAI+TALCAKGHAKRAEGVVLHH+DKISG+ SCIYRSLLYGW +Q N KEA
Sbjct: 188 KCPHDQFTVTAIITALCAKGHAKRAEGVVLHHKDKISGTMSCIYRSLLYGWSIQKNTKEA 247

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLF YNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSY I+PNSIS
Sbjct: 248 RRILKEMKSDGTMPDLFSYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYSIAPNSIS 307

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGR RRVKESCRILETMKR+GCR D +SYYLVARVLFL+GRFGKG RIV+EMI
Sbjct: 308 YNILLSCLGRARRVKESCRILETMKRTGCRPDCISYYLVARVLFLSGRFGKGCRIVEEMI 367

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGLIPD+KFYYDLIGILCGVER  YALE+ +KMKRSSLGGYGPVYDVLIPKLC GGDFE
Sbjct: 368 EEGLIPDRKFYYDLIGILCGVEREKYALELFEKMKRSSLGGYGPVYDVLIPKLCSGGDFE 427

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLW EAMAMG+ LRCS +VLDPSITKV+ PTRK D++ LEE N AEKQN+AA TD+ 
Sbjct: 428 MGRQLWGEAMAMGITLRCSSDVLDPSITKVFKPTRKIDNQNLEECNRAEKQNKAA-TDKS 487

Query: 481 KAKGKKSKNLS 491
           KA+GKKSKNLS
Sbjct: 488 KARGKKSKNLS 497

BLAST of MC06g_new0049 vs. NCBI nr
Match: KAG6589519.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 825 bits (2132), Expect = 5.72e-299
Identity = 411/491 (83.71%), Postives = 442/491 (90.02%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           MNAR MFDE  LRRCVS LKLKW     QS+ T  RFCSLHSTV N ASV  LC+VVSC+
Sbjct: 8   MNARQMFDERSLRRCVSLLKLKWDCLFVQSVCTLHRFCSLHSTVKNGASVSKLCEVVSCT 67

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELE++LNKCTVSLTS LVTQVIDSCKNEAP RRLLRFF WSLK L HDLEDEDFN
Sbjct: 68  IGGLDELEATLNKCTVSLTSSLVTQVIDSCKNEAPNRRLLRFFSWSLKKLNHDLEDEDFN 127

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIRLFAQKKDY AIDILLSDLKKA RAM+G+TFGLVAEALVK+DREDEALGLFKNLDKY
Sbjct: 128 YAIRLFAQKKDYTAIDILLSDLKKARRAMDGQTFGLVAEALVKMDREDEALGLFKNLDKY 187

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTVTAI+TALCAKGHAKRAEGVVLHH+DKISG+ SCIYRSLLYGW +Q N KEA
Sbjct: 188 KCPHDQFTVTAIITALCAKGHAKRAEGVVLHHKDKISGTMSCIYRSLLYGWSIQKNTKEA 247

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLF YNTFLRCL EQNLKKNPSGLVPEALNVMMEMRSY I+PNSIS
Sbjct: 248 RRILKEMKSDGTMPDLFSYNTFLRCLSEQNLKKNPSGLVPEALNVMMEMRSYSIAPNSIS 307

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGR RRVKESCRILETMKR+GCR D +SYYLVARVLFL+GRFGKG RIV+EMI
Sbjct: 308 YNILLSCLGRARRVKESCRILETMKRTGCRPDCISYYLVARVLFLSGRFGKGCRIVEEMI 367

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGLIPD+KFYYDLIGILCGVE   YALE+ +KMKRSSLGGYGPVYDVLIPKLCRGGDFE
Sbjct: 368 EEGLIPDRKFYYDLIGILCGVESEKYALELFEKMKRSSLGGYGPVYDVLIPKLCRGGDFE 427

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMGL LRCS +VLDPSITKV+ PTRK D++ LEE + AEKQN+AA TD+ 
Sbjct: 428 MGRQLWEEAMAMGLTLRCSSDVLDPSITKVFKPTRKIDNQNLEESSRAEKQNKAA-TDKS 487

Query: 481 KAKGKKSKNLS 491
           KA+GK+SKNLS
Sbjct: 488 KARGKRSKNLS 497

BLAST of MC06g_new0049 vs. NCBI nr
Match: XP_022921576.1 (pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Cucurbita moschata])

HSP 1 Score: 823 bits (2126), Expect = 4.69e-298
Identity = 410/491 (83.50%), Postives = 441/491 (89.82%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           MNAR MFDE  LRRCVS LKLKW     QS+ T  RFCSLHSTV N ASV  LC+VVSC+
Sbjct: 8   MNARQMFDERSLRRCVSLLKLKWDCLFVQSVCTQHRFCSLHSTVKNGASVSKLCEVVSCT 67

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELE++LNK TVSLTS LVTQVIDSCKNEAP RRLLRFF WSLK L HDLEDEDFN
Sbjct: 68  IGGLDELEATLNKSTVSLTSSLVTQVIDSCKNEAPNRRLLRFFSWSLKKLNHDLEDEDFN 127

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIRLFAQKKDY AIDILLSDLKKA RAM+G+TFGLVAEALVK+DREDEALGLFKNLDKY
Sbjct: 128 YAIRLFAQKKDYTAIDILLSDLKKARRAMDGQTFGLVAEALVKMDREDEALGLFKNLDKY 187

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTVTAI+TALCAKGHAKRAEGVVLHH+DKISG+ SCIYRSLLYGW +Q N KEA
Sbjct: 188 KCPHDQFTVTAIITALCAKGHAKRAEGVVLHHKDKISGTMSCIYRSLLYGWSIQKNTKEA 247

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLF YNTFLRCL EQNLKKNPSGLVPEALNVMMEMRSY I+PNSIS
Sbjct: 248 RRILKEMKSDGTMPDLFSYNTFLRCLSEQNLKKNPSGLVPEALNVMMEMRSYSIAPNSIS 307

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGR RRVKESCRILETMKR+GCR D +SYYLVARVLFL+GRFGKG RIV+EMI
Sbjct: 308 YNILLSCLGRARRVKESCRILETMKRTGCRPDCISYYLVARVLFLSGRFGKGCRIVEEMI 367

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGLIPD+KFYYDLIGILCGVE   YALE+ +KMKRSSLGGYGPVYDVLIPKLCRGGDFE
Sbjct: 368 EEGLIPDRKFYYDLIGILCGVESEKYALELFEKMKRSSLGGYGPVYDVLIPKLCRGGDFE 427

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMGL LRCS +VLDPSITKV+ PTRK D++ LEE + AEKQN+AA TD+ 
Sbjct: 428 MGRQLWEEAMAMGLTLRCSSDVLDPSITKVFKPTRKIDNQNLEESSRAEKQNKAA-TDKS 487

Query: 481 KAKGKKSKNLS 491
           KA+GK+SKNLS
Sbjct: 488 KARGKRSKNLS 497

BLAST of MC06g_new0049 vs. NCBI nr
Match: XP_023516447.1 (pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 820 bits (2117), Expect = 1.10e-296
Identity = 408/491 (83.10%), Postives = 440/491 (89.61%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           M+AR MFDE+ LRRCVS  KLKW SF  QS+ T  RFCSLHSTV N  SV  LC+VVSC+
Sbjct: 8   MHARQMFDEMSLRRCVSLFKLKWASFFVQSVCTGHRFCSLHSTVRNGDSVSKLCEVVSCT 67

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELE +LNK  VSLTS LVTQVIDSCKNEAP RRLLRFF WSLK L HDLEDEDFN
Sbjct: 68  IGGLDELEGTLNKFPVSLTSSLVTQVIDSCKNEAPNRRLLRFFSWSLKKLNHDLEDEDFN 127

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIRLFAQKKDY AIDILLSDLKKA RAM+G+TFGLVAEALVK+ REDEALGLFKNLDKY
Sbjct: 128 YAIRLFAQKKDYTAIDILLSDLKKARRAMDGQTFGLVAEALVKMGREDEALGLFKNLDKY 187

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTVTAI+TALCAKGHAKRAEGVVLHH+DKISG+ SCIYRSLLYGW +Q N KEA
Sbjct: 188 KCPHDQFTVTAIITALCAKGHAKRAEGVVLHHKDKISGTMSCIYRSLLYGWSIQKNTKEA 247

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLF +NTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSY I+PNSIS
Sbjct: 248 RRILKEMKSDGTMPDLFSFNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYNIAPNSIS 307

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGR RRVKESCRILETMKR+GCR D +SYYLVARVLFL+GRFGKG RIV+EMI
Sbjct: 308 YNILLSCLGRARRVKESCRILETMKRTGCRPDCISYYLVARVLFLSGRFGKGCRIVEEMI 367

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGLIPD+KFYYDLIGILCGVE   YALE+ +KMKRSSLGGYGPVYDVLIPKLCRGGDFE
Sbjct: 368 EEGLIPDRKFYYDLIGILCGVESEKYALELFEKMKRSSLGGYGPVYDVLIPKLCRGGDFE 427

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMGL LRCS +VLDPSITKV+ PTRK D++ LEE + AEKQN+AA TD+ 
Sbjct: 428 MGRQLWEEAMAMGLTLRCSSDVLDPSITKVFKPTRKIDNQGLEESSRAEKQNKAA-TDKS 487

Query: 481 KAKGKKSKNLS 491
           KA+GKKSKNLS
Sbjct: 488 KARGKKSKNLS 497

BLAST of MC06g_new0049 vs. ExPASy TrEMBL
Match: A0A6J1BYQ1 (pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111006837 PE=4 SV=1)

HSP 1 Score: 991 bits (2562), Expect = 0.0
Identity = 492/493 (99.80%), Postives = 492/493 (99.80%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS
Sbjct: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN
Sbjct: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY
Sbjct: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA
Sbjct: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS
Sbjct: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI
Sbjct: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           DEGLIPD KFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE
Sbjct: 361 DEGLIPDXKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP
Sbjct: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480

Query: 481 KAKGKKSKNLSLK 493
           KAKGKKSKNLSLK
Sbjct: 481 KAKGKKSKNLSLK 493

BLAST of MC06g_new0049 vs. ExPASy TrEMBL
Match: A0A6J1JIV3 (pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484976 PE=4 SV=1)

HSP 1 Score: 835 bits (2157), Expect = 4.32e-303
Identity = 413/491 (84.11%), Postives = 445/491 (90.63%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           M+ARHMFDE+ LRRCVS LKLKW SF  QS+ T  RFCSLHSTV N ASV  LC+VVSC+
Sbjct: 8   MHARHMFDEMSLRRCVSLLKLKWDSFFVQSVCTQHRFCSLHSTVKNGASVSKLCEVVSCT 67

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELE++LNKCTVSLTS LVTQVIDSCKNEAP RRLLRFF WSLK L H LEDEDFN
Sbjct: 68  IGGLDELEATLNKCTVSLTSSLVTQVIDSCKNEAPNRRLLRFFSWSLKKLNHHLEDEDFN 127

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIRLFAQKKDY AIDILLSDLKKA RAM+G+TFGLVAEALVK+DREDEALGLFKNLDKY
Sbjct: 128 YAIRLFAQKKDYTAIDILLSDLKKARRAMDGQTFGLVAEALVKMDREDEALGLFKNLDKY 187

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTVTAI+TALCAKGHAKRAEGVVLHH+DKISG+ SCIYRSLLYGW +Q N KEA
Sbjct: 188 KCPHDQFTVTAIITALCAKGHAKRAEGVVLHHKDKISGTMSCIYRSLLYGWSIQKNTKEA 247

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLF YNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSY I+PNSIS
Sbjct: 248 RRILKEMKSDGTMPDLFSYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYSIAPNSIS 307

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGR RRVKESCRILETMKR+GCR D +SYYLVARVLFL+GRFGKG RIV+EMI
Sbjct: 308 YNILLSCLGRARRVKESCRILETMKRTGCRPDCISYYLVARVLFLSGRFGKGCRIVEEMI 367

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGLIPD+KFYYDLIGILCGVER  YALE+ +KMKRSSLGGYGPVYDVLIPKLC GGDFE
Sbjct: 368 EEGLIPDRKFYYDLIGILCGVEREKYALELFEKMKRSSLGGYGPVYDVLIPKLCSGGDFE 427

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLW EAMAMG+ LRCS +VLDPSITKV+ PTRK D++ LEE N AEKQN+AA TD+ 
Sbjct: 428 MGRQLWGEAMAMGITLRCSSDVLDPSITKVFKPTRKIDNQNLEECNRAEKQNKAA-TDKS 487

Query: 481 KAKGKKSKNLS 491
           KA+GKKSKNLS
Sbjct: 488 KARGKKSKNLS 497

BLAST of MC06g_new0049 vs. ExPASy TrEMBL
Match: A0A6J1E1S0 (pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111429800 PE=4 SV=1)

HSP 1 Score: 823 bits (2126), Expect = 2.27e-298
Identity = 410/491 (83.50%), Postives = 441/491 (89.82%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           MNAR MFDE  LRRCVS LKLKW     QS+ T  RFCSLHSTV N ASV  LC+VVSC+
Sbjct: 8   MNARQMFDERSLRRCVSLLKLKWDCLFVQSVCTQHRFCSLHSTVKNGASVSKLCEVVSCT 67

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELE++LNK TVSLTS LVTQVIDSCKNEAP RRLLRFF WSLK L HDLEDEDFN
Sbjct: 68  IGGLDELEATLNKSTVSLTSSLVTQVIDSCKNEAPNRRLLRFFSWSLKKLNHDLEDEDFN 127

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIRLFAQKKDY AIDILLSDLKKA RAM+G+TFGLVAEALVK+DREDEALGLFKNLDKY
Sbjct: 128 YAIRLFAQKKDYTAIDILLSDLKKARRAMDGQTFGLVAEALVKMDREDEALGLFKNLDKY 187

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTVTAI+TALCAKGHAKRAEGVVLHH+DKISG+ SCIYRSLLYGW +Q N KEA
Sbjct: 188 KCPHDQFTVTAIITALCAKGHAKRAEGVVLHHKDKISGTMSCIYRSLLYGWSIQKNTKEA 247

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLF YNTFLRCL EQNLKKNPSGLVPEALNVMMEMRSY I+PNSIS
Sbjct: 248 RRILKEMKSDGTMPDLFSYNTFLRCLSEQNLKKNPSGLVPEALNVMMEMRSYSIAPNSIS 307

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCLGR RRVKESCRILETMKR+GCR D +SYYLVARVLFL+GRFGKG RIV+EMI
Sbjct: 308 YNILLSCLGRARRVKESCRILETMKRTGCRPDCISYYLVARVLFLSGRFGKGCRIVEEMI 367

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGLIPD+KFYYDLIGILCGVE   YALE+ +KMKRSSLGGYGPVYDVLIPKLCRGGDFE
Sbjct: 368 EEGLIPDRKFYYDLIGILCGVESEKYALELFEKMKRSSLGGYGPVYDVLIPKLCRGGDFE 427

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMGL LRCS +VLDPSITKV+ PTRK D++ LEE + AEKQN+AA TD+ 
Sbjct: 428 MGRQLWEEAMAMGLTLRCSSDVLDPSITKVFKPTRKIDNQNLEESSRAEKQNKAA-TDKS 487

Query: 481 KAKGKKSKNLS 491
           KA+GK+SKNLS
Sbjct: 488 KARGKRSKNLS 497

BLAST of MC06g_new0049 vs. ExPASy TrEMBL
Match: A0A0A0LPF4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009920 PE=4 SV=1)

HSP 1 Score: 812 bits (2097), Expect = 4.07e-294
Identity = 393/488 (80.53%), Postives = 440/488 (90.16%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           M+ARH+FDE+ LRRCVS LKLKW SFI QS+ T  RFCSLHSTV N A+V  LC+V+SC+
Sbjct: 1   MHARHLFDEMSLRRCVSLLKLKWDSFIAQSVCTQHRFCSLHSTVNNGAAVSKLCEVISCT 60

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELESSLNKCT+SLTS LVTQVIDS KNEAPTRRLLRFFLWSLK L H LEDEDFN
Sbjct: 61  IGGLDELESSLNKCTISLTSSLVTQVIDSSKNEAPTRRLLRFFLWSLKKLNHTLEDEDFN 120

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIR FAQKKDY A++ILLS+LKKA RAM+G+TFG VAEA VK+DREDEALGLFKNL+KY
Sbjct: 121 NAIRFFAQKKDYTAVNILLSNLKKADRAMDGQTFGFVAEAFVKMDREDEALGLFKNLEKY 180

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTV AI+TALC+KGHAKRAEGVVLHH+DKIS + SCIYRSLLYGW ++ N KEA
Sbjct: 181 KCPHDQFTVVAIITALCSKGHAKRAEGVVLHHKDKISSTMSCIYRSLLYGWSIKKNTKEA 240

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLFCYNTFL+CLCE+N++KNPSGLVPE+LNVMMEMRSYKISPNSIS
Sbjct: 241 RRILKEMKSDGTMPDLFCYNTFLKCLCEKNVEKNPSGLVPESLNVMMEMRSYKISPNSIS 300

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCL +TRRVKESC+ILE MKR+GC+ D VSYYL+ARVLFLTGRFGKGR IVDEMI
Sbjct: 301 YNILLSCLCKTRRVKESCKILEMMKRTGCQPDCVSYYLMARVLFLTGRFGKGREIVDEMI 360

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGL PD+KFYYDLIGILCGVER NYALE+ +KMKRSSLGGYGPVYDVLIPKLCRGG+FE
Sbjct: 361 EEGLTPDRKFYYDLIGILCGVERTNYALELFEKMKRSSLGGYGPVYDVLIPKLCRGGEFE 420

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMG+ L CS E+LDPSITKV+ PTRK ++KI+EE N AEKQN+AAA ++P
Sbjct: 421 MGRQLWEEAMAMGVSLNCSSEILDPSITKVFKPTRKIENKIVEEFNSAEKQNKAAA-EKP 480

Query: 481 KAKGKKSK 488
           K K KK K
Sbjct: 481 KEKRKKGK 487

BLAST of MC06g_new0049 vs. ExPASy TrEMBL
Match: A0A5A7US52 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002610 PE=4 SV=1)

HSP 1 Score: 805 bits (2079), Expect = 2.16e-291
Identity = 391/486 (80.45%), Postives = 437/486 (89.92%), Query Frame = 0

Query: 1   MNARHMFDEIPLRRCVSALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCS 60
           M+ARHMFDE+PLRRCVS LKLKW SFI QS+ T  RFCSLHSTV N A+V  LC+V+SC+
Sbjct: 1   MHARHMFDEMPLRRCVSLLKLKWDSFIAQSVCTQHRFCSLHSTVNNGAAVSKLCEVISCT 60

Query: 61  IGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFN 120
           IGGLDELESSLN+CT+SLTS LVTQVIDS KNEAPTRRLLRFFLWSLK L H LEDEDFN
Sbjct: 61  IGGLDELESSLNQCTISLTSSLVTQVIDSSKNEAPTRRLLRFFLWSLKKLNHTLEDEDFN 120

Query: 121 HAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKY 180
           +AIR FAQKKDY AI+ILLS+LKKA RAM+G+TF  VAEA VK++R+DEALGLFKNL+KY
Sbjct: 121 NAIRFFAQKKDYTAINILLSNLKKADRAMDGQTFSFVAEAFVKMNRDDEALGLFKNLEKY 180

Query: 181 KCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEA 240
           KCP DQFTV AI+TALC+KGHAKRAEGVVLHH+DKIS + SCIYRSLLYGW ++ N KEA
Sbjct: 181 KCPHDQFTVVAIITALCSKGHAKRAEGVVLHHKDKISSTMSCIYRSLLYGWSIKKNTKEA 240

Query: 241 RRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSIS 300
           RRILKEMKSDG MPDLF YNTFL+CLCE+N++KNPSGLVPEALNVMMEMRSYKI+PNSIS
Sbjct: 241 RRILKEMKSDGTMPDLFSYNTFLKCLCEKNVEKNPSGLVPEALNVMMEMRSYKIAPNSIS 300

Query: 301 YNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMI 360
           YNILLSCL +TRRVKESCRILE MKRSGCR D VSYYLVARVLFLTGRFGKGR IVDEMI
Sbjct: 301 YNILLSCLCKTRRVKESCRILEMMKRSGCRPDCVSYYLVARVLFLTGRFGKGREIVDEMI 360

Query: 361 DEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFE 420
           +EGL PD+KFYY+LIGILCGVER NYA+E+ +KMKRSSLGGYGPVYDVLIPK+CRGGDFE
Sbjct: 361 EEGLTPDRKFYYELIGILCGVERTNYAVELFEKMKRSSLGGYGPVYDVLIPKVCRGGDFE 420

Query: 421 MGRQLWEEAMAMGLKLRCSREVLDPSITKVWLPTRKTDSKILEEVNGAEKQNQAAATDRP 480
           MGRQLWEEAMAMG+ L CS E+LDPSITKV+ PTRK ++KI+EE N AEKQN+AAA ++P
Sbjct: 421 MGRQLWEEAMAMGVSLNCSSEILDPSITKVFKPTRKIENKIVEECNTAEKQNKAAA-EKP 480

Query: 481 KAKGKK 486
             K KK
Sbjct: 481 NKKRKK 485

BLAST of MC06g_new0049 vs. TAIR 10
Match: AT5G61370.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 568.2 bits (1463), Expect = 6.6e-162
Identity = 272/482 (56.43%), Postives = 373/482 (77.39%), Query Frame = 0

Query: 17  SALKLKWQSFITQSISTDLRFCSLHSTVTNAASVVDLCKVVSCSIGGLDELESSLNKCTV 76
           + ++L   +++T +      FCS H    +  ++ ++ ++VS  +GGLD+LE +LN+ +V
Sbjct: 4   TTVRLNRFTYLTSTAKLTRYFCSHHLVDRSETALHEVIRIVSSPVGGLDDLEENLNQVSV 63

Query: 77  SLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFNHAIRLFAQKKDYNAID 136
           S +S LVTQVI+SCKNE   RRLLRFF WS K+L   L D++FN+ +R+ A+KKD+ A+ 
Sbjct: 64  SPSSNLVTQVIESCKNETSPRRLLRFFSWSCKSLGSSLHDKEFNYVLRVLAEKKDHTAMQ 123

Query: 137 ILLSDLKKAGRAMEGETFGLVAEALVKLDREDEALGLFKNLDKYKCPQDQFTVTAIVTAL 196
           ILLSDL+K  RAM+ +TF +VAE LVK+ +E++A+G+FK LDK+ CPQD FTVTAI++AL
Sbjct: 124 ILLSDLRKENRAMDKQTFSIVAETLVKVGKEEDAIGIFKILDKFSCPQDGFTVTAIISAL 183

Query: 197 CAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEARRILKEMKSDGAMPDL 256
           C++GH KRA GV+ HH+D ISG++  +YRSLL+GW VQ N KEARR++++MKS G  PDL
Sbjct: 184 CSRGHVKRALGVMHHHKDVISGNELSVYRSLLFGWSVQRNVKEARRVIQDMKSAGITPDL 243

Query: 257 FCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSISYNILLSCLGRTRRVKE 316
           FC+N+ L CLCE+N+ +NPSGLVPEALN+M+EMRSYKI P S+SYNILLSCLGRTRRV+E
Sbjct: 244 FCFNSLLTCLCERNVNRNPSGLVPEALNIMLEMRSYKIQPTSMSYNILLSCLGRTRRVRE 303

Query: 317 SCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMIDEGLIPDQKFYYDLIG 376
           SC+ILE MKRSGC  D  SYY V RVL+LTGRFGKG +IVDEMI+ G  P++KFYYDLIG
Sbjct: 304 SCQILEQMKRSGCDPDTGSYYFVVRVLYLTGRFGKGNQIVDEMIERGFRPERKFYYDLIG 363

Query: 377 ILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIPKLCRGGDFEMGRQLWEEAMAMGLKL 436
           +LCGVERVN+AL++ +KMKRSS+GGYG VYD+LIPKLC+GG+FE GR+LWEEA+++ + L
Sbjct: 364 VLCGVERVNFALQLFEKMKRSSVGGYGQVYDLLIPKLCKGGNFEKGRELWEEALSIDVTL 423

Query: 437 RCSREVLDPSITKVWLPTR-KTDSKILE------EVNGAEKQNQAAATDRPKAKGK-KSK 491
            CS  +LDPS+T+V+ P + K ++ +++      +++    + +     +PK + K K K
Sbjct: 424 SCSISLLDPSVTEVFKPMKMKEEAAMVDRRALNLKIHARMNKTKPKLKLKPKRRSKTKKK 483

BLAST of MC06g_new0049 vs. TAIR 10
Match: AT5G60960.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 7.6e-41
Identity = 105/377 (27.85%), Postives = 180/377 (47.75%), Query Frame = 0

Query: 97  RRLLRFFLWSLKNLKHDLEDEDFNHAIRLFAQKKDYNAIDILLSDLKKAGRAMEGETFGL 156
           R  L F  W   N      DE  +  +  F ++KD+     +L  + K      G+T   
Sbjct: 124 RAALGFNEWLDSNSNFSHTDETVSFFVDYFGRRKDFKG---MLEIISKYKGIAGGKTLES 183

Query: 157 VAEALVKLDREDEALGLFKNLDK-YKCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDK 216
             + LV+  R  +    F+ ++  Y   +D+ ++T +V  LC KGHA  AE +V +  ++
Sbjct: 184 AIDRLVRAGRPKQVTDFFEKMENDYGLKRDKESLTLVVKKLCEKGHASIAEKMVKNTANE 243

Query: 217 ISGSKSCIYRSLLYGWYVQNNAKEARRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNP 276
           I   ++ I   L+ GW +     EA R+  EM   G       YN  L C+C+   KK+P
Sbjct: 244 IFPDEN-ICDLLISGWCIAEKLDEATRLAGEMSRGGFEIGTKAYNMMLDCVCKLCRKKDP 303

Query: 277 SGLVPEALNVMMEMRSYKISPNSISYNILLSCLGRTRRVKESCRILETMKRSGCRADWVS 336
             L PE   V++EM    +  N+ ++N+L++ L + RR +E+  +   M   GC+ D  +
Sbjct: 304 FKLQPEVEKVLLEMEFRGVPRNTETFNVLINNLCKIRRTEEAMTLFGRMGEWGCQPDAET 363

Query: 337 YYLVARVLFLTGRFGKGRRIVDEMIDEGL--IPDQKFYYDLIGILCGVERVNYALEMLDK 396
           Y ++ R L+   R G+G  ++D+M   G   + ++K YY  + ILCG+ER+ +A+ +   
Sbjct: 364 YLVLIRSLYQAARIGEGDEMIDKMKSAGYGELLNKKEYYGFLKILCGIERLEHAMSVFKS 423

Query: 397 MKRSSLGGYGPVYDVLIPKLCRGGDFEMGRQLWEEAMAMGLKLRCSREVLDPSITKVWLP 456
           MK +        YD+L+ K+C          L++EA   G+ +      +DP   K    
Sbjct: 424 MKANGCKPGIKTYDLLMGKMCANNQLTRANGLYKEAAKKGIAVSPKEYRVDPRFMK--KK 483

Query: 457 TRKTDSKILEEVNGAEK 471
           T++ DS + +     EK
Sbjct: 484 TKEVDSNVKKRETLPEK 494

BLAST of MC06g_new0049 vs. TAIR 10
Match: AT3G49730.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 6.7e-37
Identity = 101/390 (25.90%), Postives = 182/390 (46.67%), Query Frame = 0

Query: 47  AASVVDLCKVVSCSIGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWS 106
           A  V  + +++      + +LE +LN+  + L   L+ +V+  C +        RFFLW+
Sbjct: 64  AGEVEKIYRILRNHHSRVPKLELALNESGIDLRPGLIIRVLSRCGDAGNLG--YRFFLWA 123

Query: 107 LKNLKHDLEDEDFNHAIRLFAQKKDYNAIDILLSDLKKAG-RAMEGETFGLVAEALVKLD 166
            K   +    E     + + ++ + + A+  L+ +++K     +E E F ++       +
Sbjct: 124 TKQPGYFHSYEVCKSMVMILSKMRQFGAVWGLIEEMRKTNPELIEPELFVVLMRRFASAN 183

Query: 167 REDEALGLFKNLDKYKCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYR 226
              +A+ +   + KY    D++    ++ ALC  G  K A  V    R+K   +    + 
Sbjct: 184 MVKKAVEVLDEMPKYGLEPDEYVFGCLLDALCKNGSVKEASKVFEDMREKFPPNLR-YFT 243

Query: 227 SLLYGWYVQNNAKEARRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNV 286
           SLLYGW  +    EA+ +L +MK  G  PD+  +   L            +G + +A ++
Sbjct: 244 SLLYGWCREGKLMEAKEVLVQMKEAGLEPDIVVFTNLLSGYAH-------AGKMADAYDL 303

Query: 287 MMEMRSYKISPNSISYNILLSCLGRT-RRVKESCRILETMKRSGCRADWVSYYLVARVLF 346
           M +MR     PN   Y +L+  L RT +R+ E+ R+   M+R GC AD V+Y  +     
Sbjct: 304 MNDMRKRGFEPNVNCYTVLIQALCRTEKRMDEAMRVFVEMERYGCEADIVTYTALISGFC 363

Query: 347 LTGRFGKGRRIVDEMIDEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGP 406
             G   KG  ++D+M  +G++P Q  Y  ++      E+    LE+++KMKR        
Sbjct: 364 KWGMIDKGYSVLDDMRKKGVMPSQVTYMQIMVAHEKKEQFEECLELIEKMKRRGCHPDLL 423

Query: 407 VYDVLIPKLCRGGDFEMGRQLWEEAMAMGL 435
           +Y+V+I   C+ G+ +   +LW E  A GL
Sbjct: 424 IYNVVIRLACKLGEVKEAVRLWNEMEANGL 443

BLAST of MC06g_new0049 vs. TAIR 10
Match: AT3G22670.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 5.8e-25
Identity = 82/355 (23.10%), Postives = 159/355 (44.79%), Query Frame = 0

Query: 65  DELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWSLKNLKHDLEDEDFNHAIR 124
           +++   L+KC V +T  LV QV+    N     +   FF+W+     +      +N  + 
Sbjct: 117 EDVVKELSKCDVVVTESLVLQVLRRFSN--GWNQAYGFFIWANSQTGYVHSGHTYNAMVD 176

Query: 125 LFAQKKDYNAIDILLSDLKK--AGRAMEGETFGLVAEALVKLDREDEALGLFKNLDK-YK 184
           +  + ++++ +  L++++ K    + +  +T   V   L K  + ++A+  F  ++K Y 
Sbjct: 177 VLGKCRNFDLMWELVNEMNKNEESKLVTLDTMSKVMRRLAKSGKYNKAVDAFLEMEKSYG 236

Query: 185 CPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRSLLYGWYVQNNAKEAR 244
              D   + +++ AL  +   + A  V L   D I    +  +  L++G+       +AR
Sbjct: 237 VKTDTIAMNSLMDALVKENSIEHAHEVFLKLFDTIK-PDARTFNILIHGFCKARKFDDAR 296

Query: 245 RILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVMMEMRSYKISPNSISY 304
            ++  MK     PD+  Y +F+   C++       G       ++ EMR    +PN ++Y
Sbjct: 297 AMMDLMKVTEFTPDVVTYTSFVEAYCKE-------GDFRRVNEMLEEMRENGCNPNVVTY 356

Query: 305 NILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLTGRFGKGRRIVDEMID 364
            I++  LG++++V E+  + E MK  GC  D   Y  +  +L  TGRF     I ++M +
Sbjct: 357 TIVMHSLGKSKQVAEALGVYEKMKEDGCVPDAKFYSSLIHILSKTGRFKDAAEIFEDMTN 416

Query: 365 EGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVYDVLIP--KLC 415
           +G+  D   Y  +I       R   AL +L +M+        P  +   P  K+C
Sbjct: 417 QGVRRDVLVYNTMISAALHHSRDEMALRLLKRMEDEEGESCSPNVETYAPLLKMC 461

BLAST of MC06g_new0049 vs. TAIR 10
Match: AT1G52640.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-24
Identity = 89/381 (23.36%), Postives = 164/381 (43.04%), Query Frame = 0

Query: 50  VVDLCKVVSCSIGGLDELESSLNKCTVSLTSPLVTQVIDSCKNEAPTRRLLRFFLWS--L 109
           V ++ +V+S      D+LE +L   +  ++S LV QV+  CKN        RFFLW+  +
Sbjct: 38  VNEISRVLSDHRNPKDDLEHTLVAYSPRVSSNLVEQVLKRCKNLGFPAH--RFFLWARRI 97

Query: 110 KNLKHDLEDEDFNHAIRLFAQKKDYNAI-DILLSDLKKAGRAMEGETFGLVAEALVKLDR 169
            +  H L  E ++  + +    K +  + D L+   +     +  + F +V  A  + + 
Sbjct: 98  PDFAHSL--ESYHILVEILGSSKQFALLWDFLIEAREYNYFEISSKVFWIVFRAYSRANL 157

Query: 170 EDEALGLFKNLDKYKCPQDQFTVTAIVTALCAKGHAKRAEGVVLHHRDKISGSKSCIYRS 229
             EA   F  + ++        +  ++ +LC K H   A+      +       +  Y  
Sbjct: 158 PSEACRAFNRMVEFGIKPCVDDLDQLLHSLCDKKHVNHAQEFFGKAKGFGIVPSAKTYSI 217

Query: 230 LLYGWYVQNNAKEARRILKEMKSDGAMPDLFCYNTFLRCLCEQNLKKNPSGLVPEALNVM 289
           L+ GW    +A  AR++  EM     + DL  YN  L  LC+       SG V     + 
Sbjct: 218 LVRGWARIRDASGARKVFDEMLERNCVVDLLAYNALLDALCK-------SGDVDGGYKMF 277

Query: 290 MEMRSYKISPNSISYNILLSCLGRTRRVKESCRILETMKRSGCRADWVSYYLVARVLFLT 349
            EM +  + P++ S+ I +        V  + ++L+ MKR     +  ++  + + L   
Sbjct: 278 QEMGNLGLKPDAYSFAIFIHAYCDAGDVHSAYKVLDRMKRYDLVPNVYTFNHIIKTLCKN 337

Query: 350 GRFGKGRRIVDEMIDEGLIPDQKFYYDLIGILCGVERVNYALEMLDKMKRSSLGGYGPVY 409
            +      ++DEMI +G  PD   Y  ++   C    VN A ++L +M R+        Y
Sbjct: 338 EKVDDAYLLLDEMIQKGANPDTWTYNSIMAYHCDHCEVNRATKLLSRMDRTKCLPDRHTY 397

Query: 410 DVLIPKLCRGGDFEMGRQLWE 428
           ++++  L R G F+   ++WE
Sbjct: 398 NMVLKLLIRIGRFDRATEIWE 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLJ69.3e-16156.43Pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Arabidop... [more]
Q9FME41.1e-3927.85Pentatricopeptide repeat-containing protein PNM1, mitochondrial OS=Arabidopsis t... [more]
P0C8A09.4e-3625.90Pentatricopeptide repeat-containing protein At3g49730 OS=Arabidopsis thaliana OX... [more]
Q9LUJ48.2e-2423.10Pentatricopeptide repeat-containing protein At3g22670, mitochondrial OS=Arabidop... [more]
Q9SSR63.1e-2323.36Pentatricopeptide repeat-containing protein At1g52640, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
XP_022134610.10.099.80pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Momordica ... [more]
XP_022987424.18.92e-30384.11pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Cucurbita ... [more]
KAG6589519.15.72e-29983.71Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_022921576.14.69e-29883.50pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Cucurbita ... [more]
XP_023516447.11.10e-29683.10pentatricopeptide repeat-containing protein At5g61370, mitochondrial [Cucurbita ... [more]
Match NameE-valueIdentityDescription
A0A6J1BYQ10.099.80pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Momordic... [more]
A0A6J1JIV34.32e-30384.11pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Cucurbit... [more]
A0A6J1E1S02.27e-29883.50pentatricopeptide repeat-containing protein At5g61370, mitochondrial OS=Cucurbit... [more]
A0A0A0LPF44.07e-29480.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009920 PE=4 SV=1[more]
A0A5A7US522.16e-29180.45Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
Match NameE-valueIdentityDescription
AT5G61370.16.6e-16256.43Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G60960.17.6e-4127.85Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G49730.16.7e-3725.90Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G22670.15.8e-2523.10Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G52640.12.2e-2423.36Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 296..336
e-value: 1.9E-7
score: 31.2
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 224..264
e-value: 3.1E-4
score: 20.8
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 334..367
e-value: 0.0018
score: 16.3
coord: 299..333
e-value: 9.8E-8
score: 29.7
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 297..331
score: 11.366925
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 332..366
score: 8.516988
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 360..433
e-value: 4.7E-6
score: 28.2
coord: 50..213
e-value: 7.8E-11
score: 43.8
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 214..359
e-value: 7.6E-26
score: 93.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 464..493
NoneNo IPR availablePANTHERPTHR47938RESPIRATORY COMPLEX I CHAPERONE (CIA84), PUTATIVE (AFU_ORTHOLOGUE AFUA_2G06020)-RELATEDcoord: 32..479
NoneNo IPR availablePANTHERPTHR47938:SF9PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN MITOCHONDRIALcoord: 32..479

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g_new0049.1MC06g_new0049.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding