MC06g_new0033 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g_new0033
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Major facilitator superfamily protein
Location	MC06: 1340668 .. 1342153 (-)
RNA-Seq Expression	MC06g_new0033
Synteny	MC06g_new0033

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGAGCCCGACGACGGCGCACGTGTGGGCCTTAGTCTTGACTTTTCTCACACCAAGTTTATAAGTTCAATACTGAGTCAATGCTTATAAACCAACTTCCCAAAAGGTTATTCTTCCGATGTGGGACAACAAAAAGTTTTAATATTTGTCATTCCCAACAATCTTGTGCTGCTGTCGATCTTGCCGGCGCTTTCGACTCTTCTTCTCATGGCCTTCGTCGTAATCGACAAAACAGAGACCGGAAACGAATCAAACCATTTGAACTCCTTCTCAACAATTGCTCTCATAATCTCCGCTTATCTCACTATTCTCATCATTTTGAACAACGCCCTCACTTTACCAATCTGGGCTCGCATCTTCACCTTCGTTCTACTCCTAACCCTAATCGCCTCCCCTCTCGGAATCGCAATCAGAGCACGAACAGAGGACTCCGTTTTCCTTCAAAACGCCGACGATTCCGTCGAATACCGTCAAATTCCTAGTGAAGTTCAGAGCGATGATCAGCTGGCGATTGTTTCAGACGGAGAAATGAATATAATCGAGGCAATCGGCACAGTGAACTTCTGGCTACTATCTTTTGCGATGATGTGCGGGATGGGTTCTGGTTTAGCCACCATAAACAACATGAATCAATTGGGCCAATCTTTGGGTTACGGAACAGTCGAAATCAACGCATTCGTTTCACTGTGGAGCATATGGAATTTCCTTGGCCGTCTAGGGCCCGGCTATGCTTCTGATCTTCTCCTCCGGCAACTAGGCTTGGCGCGGCCATTTTTAATGGCGGCGGCCTTGCTGACCATGAGCATCGGCCATATCATCATAGCTTCAGGATTCCCTGGGAATTTGTACGTGGGATCGGTGATCGTGGGGATCTGTTACGGCTCTCAGTGGTCGCTGATGCCGGCGATTACGGCGGAGATATTTGGAATTAGGAATATGGGTACCATTTTCAATACCATCACTGTAGCCAGTCCGATTGCATCTTATATTCTATCTGTTAGAGTTATTGGGTATACATATGACAGAGAAGCCGGAGCCTCCGCCGGAGACAACTCTTGCTCCGGCAAACATTGCTTTATGACATCTTTTCTGGTCATGGCTGCTGTGGCTTTCTTGGGTTTTCTTGTTGCTGTTGTTCTGTTCTTCCGGACCAGGAGATTCTATCAATCTCTTGTGCAGAGAAGGCTCAAAGATTAGATGAAATCTGAAGCGTTGAGTGAAATTGGTTGTTTGTTCATATAATTTTTTTTAAAGGATTTTTTTTTTCATAGAATTTTCTTATTCTTCTTCGGAATTAATATTTTTAGTCTGTTGTTTGTAAGGTGTGAGGTATTGGATGATTGAGCGATTCAAGGTTTGAGTTGTTAAATCGGTGAAGATTTTTATTAATGAAAATTTTCACAGCTTTTTTTAAAATTAAGTAATTAAGATGTAAAATATGTTTTTAAAGTTTTGATTTTTTTTTGTTAAGTTCAATAAATTGGGG

mRNA sequence

ATGGCCTTCGTCGTAATCGACAAAACAGAGACCGGAAACGAATCAAACCATTTGAACTCCTTCTCAACAATTGCTCTCATAATCTCCGCTTATCTCACTATTCTCATCATTTTGAACAACGCCCTCACTTTACCAATCTGGGCTCGCATCTTCACCTTCGTTCTACTCCTAACCCTAATCGCCTCCCCTCTCGGAATCGCAATCAGAGCACGAACAGAGGACTCCGTTTTCCTTCAAAACGCCGACGATTCCGTCGAATACCGTCAAATTCCTAGTGAAGTTCAGAGCGATGATCAGCTGGCGATTGTTTCAGACGGAGAAATGAATATAATCGAGGCAATCGGCACAGTGAACTTCTGGCTACTATCTTTTGCGATGATGTGCGGGATGGGTTCTGGTTTAGCCACCATAAACAACATGAATCAATTGGGCCAATCTTTGGGTTACGGAACAGTCGAAATCAACGCATTCGTTTCACTGTGGAGCATATGGAATTTCCTTGGCCGTCTAGGGCCCGGCTATGCTTCTGATCTTCTCCTCCGGCAACTAGGCTTGGCGCGGCCATTTTTAATGGCGGCGGCCTTGCTGACCATGAGCATCGGCCATATCATCATAGCTTCAGGATTCCCTGGGAATTTGTACGTGGGATCGGTGATCGTGGGGATCTGTTACGGCTCTCAGTGGTCGCTGATGCCGGCGATTACGGCGGAGATATTTGGAATTAGGAATATGGGTACCATTTTCAATACCATCACTGTAGCCAGTCCGATTGCATCTTATATTCTATCTGTTAGAGTTATTGGGTATACATATGACAGAGAAGCCGGAGCCTCCGCCGGAGACAACTCTTGCTCCGGCAAACATTGCTTTATGACATCTTTTCTGGTCATGGCTGCTGTGGCTTTCTTGGGTTTTCTTGTTGCTGTTGTTCTGTTCTTCCGGACCAGGAGATTCTATCAATCTCTTGTGCAGAGAAGGCTCAAAGATTAG

Coding sequence (CDS)

ATGGCCTTCGTCGTAATCGACAAAACAGAGACCGGAAACGAATCAAACCATTTGAACTCCTTCTCAACAATTGCTCTCATAATCTCCGCTTATCTCACTATTCTCATCATTTTGAACAACGCCCTCACTTTACCAATCTGGGCTCGCATCTTCACCTTCGTTCTACTCCTAACCCTAATCGCCTCCCCTCTCGGAATCGCAATCAGAGCACGAACAGAGGACTCCGTTTTCCTTCAAAACGCCGACGATTCCGTCGAATACCGTCAAATTCCTAGTGAAGTTCAGAGCGATGATCAGCTGGCGATTGTTTCAGACGGAGAAATGAATATAATCGAGGCAATCGGCACAGTGAACTTCTGGCTACTATCTTTTGCGATGATGTGCGGGATGGGTTCTGGTTTAGCCACCATAAACAACATGAATCAATTGGGCCAATCTTTGGGTTACGGAACAGTCGAAATCAACGCATTCGTTTCACTGTGGAGCATATGGAATTTCCTTGGCCGTCTAGGGCCCGGCTATGCTTCTGATCTTCTCCTCCGGCAACTAGGCTTGGCGCGGCCATTTTTAATGGCGGCGGCCTTGCTGACCATGAGCATCGGCCATATCATCATAGCTTCAGGATTCCCTGGGAATTTGTACGTGGGATCGGTGATCGTGGGGATCTGTTACGGCTCTCAGTGGTCGCTGATGCCGGCGATTACGGCGGAGATATTTGGAATTAGGAATATGGGTACCATTTTCAATACCATCACTGTAGCCAGTCCGATTGCATCTTATATTCTATCTGTTAGAGTTATTGGGTATACATATGACAGAGAAGCCGGAGCCTCCGCCGGAGACAACTCTTGCTCCGGCAAACATTGCTTTATGACATCTTTTCTGGTCATGGCTGCTGTGGCTTTCTTGGGTTTTCTTGTTGCTGTTGTTCTGTTCTTCCGGACCAGGAGATTCTATCAATCTCTTGTGCAGAGAAGGCTCAAAGATTAG

Protein sequence

MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLIASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD

Homology

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BLAST of MC06g_new0033 vs. ExPASy Swiss-Prot
Match: Q6CGU8 (Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=MCH1 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-09
Identity = 54/187 (28.88%), Postives = 95/187 (50.80%), Query Frame = 0


Query: 141 NQLGQSLGYGTVE------INAFVSLWSIWNFLGRLGPGYASDLLLRQLGLARPFLMAAA 200
           N +G  L   TVE       +  VSL++ ++ + RL  G++S+ +  +  ++RP L++  
Sbjct: 293 NNMGAILDTVTVENADSPSFSTHVSLFATFSTVSRLVVGFSSEAM--ESHVSRPVLLSVI 352

Query: 201 LLTMSIGHIIIASG----FPGNLY--VGSVIVGICYGSQWSLMPAITAEIFGIRNMGTIF 260
            L  +  H+++ SG    F    Y  V +++ G  YGS ++L+P I  +++GI N+GTI+
Sbjct: 353 ALVAACIHLMVPSGIFTVFDNAKYFSVVTIVNGFSYGSSFTLVPTIVTKVWGIANLGTIW 412

Query: 261 NTITVASPIASY---ILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAVAFLGF 313
            +  +A  + S    +L  +V  Y    E G  +    CSG HC+  +F++      L F
Sbjct: 413 GSFILALAVGSLGYGLLFAKV--YDAASEVGVGSMSQVCSGVHCYGLTFVITG--TGLAF 472

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BLAST of MC06g_new0033 vs. ExPASy Swiss-Prot
Match: Q5AXV1 (Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=mch1 PE=3 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 1.1e-05
Identity = 50/206 (24.27%), Postives = 88/206 (42.72%), Query Frame = 0


Query: 120 WLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEI---------NAFVSLWSIWNFLGRL 179
           W L+       G G A +NN+  + Q+L   T  I         +  V++ ++ + + RL
Sbjct: 379 WWLALGFFLVTGPGEAYLNNLGTIVQTLNLDTTAIVDSHPAGLPSTHVTIIALTSTIARL 438

Query: 180 GPGYASDLLL---------------------RQLGLARPFLMAAALLTMSIGHIIIASGF 239
             G  SDL                       ++  L+R   +  + L +S+G +++AS  
Sbjct: 439 LTGSLSDLFAPTARRHFTVDQETAGPDPFTKQRPALSRLAFLIPSALLLSLGFLLLASPL 498

Query: 240 PGN----LYVGSVIVGICYGSQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVR 291
           P +     ++ + +VG+ YGS +SL+P I + ++G+ N GT +  I    P A   +   
Sbjct: 499 PTHHPELSHLTTALVGLGYGSIFSLVPIIISVVWGVENFGTNWG-IVAMFPAAGAAMWGV 558

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BLAST of MC06g_new0033 vs. ExPASy Swiss-Prot
Match: A0LNN5 (L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) OX=335543 GN=Sfum_3364 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.5e-05
Identity = 39/149 (26.17%), Postives = 67/149 (44.97%), Query Frame = 0


Query: 112 EAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLG 171
           EA G   FWLL  A  CG  +GL  I ++   G+  G   +     VS  +  N   R+ 
Sbjct: 223 EAKGDTKFWLLYLAYFCGSFAGLMVIGHLAGFGRDAGLTAMAAAGAVSSLAFSNAATRIL 282

Query: 172 PGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLM 231
            G+  D    ++G+   F    AL T ++  I    G    L + ++++G  YG+ ++L 
Sbjct: 283 SGWFVD----KIGIRVYFAALFALQTAAMIAIFQLGGSVVGLSIVAIVIGWNYGAMFTLF 342

Query: 232 PAITAEIFGIRNMGTIFNTITVASPIASY 261
           PA   + +G    G+ +  +  A  +A +
Sbjct: 343 PATCLQFYGPTAQGSNYGLLFTACGLAGF 367

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BLAST of MC06g_new0033 vs. ExPASy Swiss-Prot
Match: Q4WVT3 (Probable transporter mch1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mch1 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.4e-05
Identity = 31/107 (28.97%), Postives = 59/107 (55.14%), Query Frame = 0


Query: 189 FLMAAALLTMSIGHIIIASGF----PGNLYVGSVIVGICYGSQWSLMPAITAEIFGIRNM 248
           FL+ +ALL +S+G+++++S      PG  +V + ++G+ YGS +SL+P I + ++G+ N 
Sbjct: 482 FLLPSALL-LSLGYLLLSSPLPLQHPGLSHVTTALIGLGYGSAFSLVPIIISVVWGVENF 541

Query: 249 GTIFNTITVASPIASYILSVRVI-GYTYDREAGASAGDNSCSGKHCF 291
           GT +  + +     + +  V    GY    + G  + D  C G  C+
Sbjct: 542 GTNWGIVAMVPAAGAAMWGVIYSRGYQDATDGGNGSPDGQCHGWRCY 587

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BLAST of MC06g_new0033 vs. ExPASy Swiss-Prot
Match: Q9P3K8 (Probable transporter mch1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=mch1 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.2e-04
Identity = 58/216 (26.85%), Postives = 91/216 (42.13%), Query Frame = 0


Query: 120 WLLSFAMMCGMGSGLATINNMNQLGQSL--------GYGTVEINAFVSLWSIWNFLGRLG 179
           W  +      +G G A INN+  + ++L        G  T      VS+  I + L RL 
Sbjct: 358 WCFALGFFLMIGPGEAFINNLGTVIKTLYPPHLKFVGEPT-SAATHVSIVGITSTLVRLL 417

Query: 180 PGYASDLLL------------------RQLGLARPFLMAAALLTMSIGHIIIASGFPGN- 239
            G  +DLL                   ++  L+R   +    +T+S+G   +ASG+  N 
Sbjct: 418 TGSLTDLLAPSPQARHVQITSSGTLERKRFSLSRVSFLLFFAVTLSVGLATLASGWIQNH 477

Query: 240 ---LYVGSVIVGICYGSQWSLMPAITAEIFGIRNMGTIFNTITVASPI-ASYILSVRVIG 299
               +V S +VG  YG+ +SL P I   I+G+ N  T +  + +   + A++   V    
Sbjct: 478 GERFWVASGLVGAGYGAVFSLTPIIITVIWGVENFATNWGIVAMFPALGATFWGLVYSAV 537

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BLAST of MC06g_new0033 vs. NCBI nr
Match: XP_022134701.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 [Momordica charantia])

HSP 1 Score: 622 bits (1605), Expect = 3.27e-221
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI
Sbjct: 193 MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 252

Query: 61  ASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFW 120
           ASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFW
Sbjct: 253 ASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFW 312

Query: 121 LLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLL 180
           LLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLL
Sbjct: 313 LLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLL 372

Query: 181 RQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFG 240
           RQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFG
Sbjct: 373 RQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFG 432

Query: 241 IRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAV 300
           IRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAV
Sbjct: 433 IRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAV 492

Query: 301 AFLGFLVAVVLFFRTRRFYQSLVQRRLKD 329
           AFLGFLVAVVLFFRTRRFYQSLVQRRLKD
Sbjct: 493 AFLGFLVAVVLFFRTRRFYQSLVQRRLKD 521

[Close]

BLAST of MC06g_new0033 vs. NCBI nr
Match: XP_022933362.1 (uncharacterized protein LOC111440723 [Cucurbita moschata])

HSP 1 Score: 507 bits (1305), Expect = 9.72e-176
Identity = 271/332 (81.63%), Postives = 293/332 (88.25%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FVVI+KTE GNES HLNS S IALIIS YLTILIIL+N   LP W R+FTFVLLL L+
Sbjct: 184 MRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILL 243

Query: 61  ASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTV 120
           ASPLGIA RA+TEDSV    LQ+ D+SVEY QIP E Q +DQL +V++GEMN+IEAIGTV
Sbjct: 244 ASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTV 303

Query: 121 NFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASD 180
           NFWLL  AMMCGMGSGLATINNMNQLGQSLGY TVEI+ FVSLWSIWNFLGRLG GYASD
Sbjct: 304 NFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASD 363

Query: 181 LLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAE 240
           LLLR+LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYGSQWSLMPAITAE
Sbjct: 364 LLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAE 423

Query: 241 IFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVM 300
           IFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDREA  SAGDNSCSGKHCFMTSFL+M
Sbjct: 424 IFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREA--SAGDNSCSGKHCFMTSFLIM 483

Query: 301 AAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 329
           AAVAFLGF+VAV LF RTRRFYQ LVQR+LKD
Sbjct: 484 AAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD 513

[Close]

BLAST of MC06g_new0033 vs. NCBI nr
Match: KAG6587515.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 504 bits (1299), Expect = 1.04e-174
Identity = 270/332 (81.33%), Postives = 292/332 (87.95%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FVVI+KTE GNES HLNS S IALIIS YLTILIIL+N   LP W R+FTFVLLL L+
Sbjct: 192 MRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFILPTWVRLFTFVLLLILL 251

Query: 61  ASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTV 120
           ASPLGIA RA+TEDSV    LQ+ D+SVEY QIP E Q +DQL +V++GEMN+IEAIGTV
Sbjct: 252 ASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTV 311

Query: 121 NFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASD 180
           NFWLL  AMMCGMGSGLATINNMNQLGQSLGY  VEI+ FVSLWSIWNFLGRLG GYASD
Sbjct: 312 NFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKPVEIHTFVSLWSIWNFLGRLGSGYASD 371

Query: 181 LLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAE 240
           LLLR+LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYGSQWSLMPAITAE
Sbjct: 372 LLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAE 431

Query: 241 IFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVM 300
           IFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDREA  SAGDNSCSGKHCFMTSFL+M
Sbjct: 432 IFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREA--SAGDNSCSGKHCFMTSFLIM 491

Query: 301 AAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 329
           AAVAFLGF+VAV LF RTRRFYQ LVQR+LKD
Sbjct: 492 AAVAFLGFVVAVALFLRTRRFYQLLVQRQLKD 521

[Close]

BLAST of MC06g_new0033 vs. NCBI nr
Match: XP_023530984.1 (uncharacterized protein LOC111793373 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497 bits (1280), Expect = 7.94e-172
Identity = 267/332 (80.42%), Postives = 289/332 (87.05%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FVVI KTE GNES HLNS S IALIIS YLTILIIL+N L LP W R+FTFV+LL L+
Sbjct: 192 MRFVVIQKTEIGNESTHLNSLSKIALIISCYLTILIILDNVLILPTWVRLFTFVILLILL 251

Query: 61  ASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTV 120
           ASPLGIA RA+TEDS     LQ+ D+SVEY QIP E Q +DQL +V++GEMN+IEAIGTV
Sbjct: 252 ASPLGIANRAQTEDSASKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTV 311

Query: 121 NFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASD 180
           NFWLL  AMMCGMGSGLATINNMNQLGQSLGY TVEI+ FVSLWSIWNFLGRLG GYASD
Sbjct: 312 NFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASD 371

Query: 181 LLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAE 240
           LLLR+LG ARP LMA ALLTMS+GHIIIASG   NLY+GSVIVGICYGSQWSLMPAITAE
Sbjct: 372 LLLRELGWARPLLMAVALLTMSVGHIIIASGSSWNLYLGSVIVGICYGSQWSLMPAITAE 431

Query: 241 IFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVM 300
           IFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDREA  SAGDNSCSGKHCFMTSFL+M
Sbjct: 432 IFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREA--SAGDNSCSGKHCFMTSFLIM 491

Query: 301 AAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 329
           AAVAFLGF+VA  LF RTRRFYQ LVQR+LKD
Sbjct: 492 AAVAFLGFVVAFALFLRTRRFYQLLVQRQLKD 521

[Close]

BLAST of MC06g_new0033 vs. NCBI nr
Match: KAG7021495.1 (Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495 bits (1274), Expect = 8.91e-159
Identity = 266/331 (80.36%), Postives = 289/331 (87.31%), Query Frame = 0


Query: 1    MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
            M FVVI+KTE GNES HLNS S IALIIS YLTILIIL+N   L  W R+FTFVLLL L+
Sbjct: 732  MRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFILTTWVRLFTFVLLLILL 791

Query: 61   ASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTV 120
            ASPLGIA RA+TEDSV    LQ+ D+SVEY QIP E Q +DQL +V++GEMN+IEAIGTV
Sbjct: 792  ASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTV 851

Query: 121  NFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASD 180
            NFWLL  AMMCGMGSGLATINNMNQLGQSLGY TVEI+ FVSLWSIWNFLGRLG GYASD
Sbjct: 852  NFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASD 911

Query: 181  LLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAE 240
            LLLR+LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYGSQWSLMPAITAE
Sbjct: 912  LLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAE 971

Query: 241  IFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVM 300
            IFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDREA  SAGDNSCSGKHCFMTSFL+M
Sbjct: 972  IFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREA--SAGDNSCSGKHCFMTSFLIM 1031

Query: 301  AAVAFLGFLVAVVLFFRTRRFYQSLVQRRLK 328
            AAVAFLGF+VAV LF RTRRFYQ L +R+ K
Sbjct: 1032 AAVAFLGFVVAVALFLRTRRFYQLLGKRKRK 1060

[Close]

BLAST of MC06g_new0033 vs. ExPASy TrEMBL
Match: A0A6J1BYJ5 (LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 OS=Momordica charantia OX=3673 GN=LOC111006908 PE=4 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 1.59e-221
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI
Sbjct: 193 MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 252

Query: 61  ASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFW 120
           ASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFW
Sbjct: 253 ASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTVNFW 312

Query: 121 LLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLL 180
           LLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLL
Sbjct: 313 LLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLL 372

Query: 181 RQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFG 240
           RQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFG
Sbjct: 373 RQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFG 432

Query: 241 IRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAV 300
           IRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAV
Sbjct: 433 IRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVMAAV 492

Query: 301 AFLGFLVAVVLFFRTRRFYQSLVQRRLKD 329
           AFLGFLVAVVLFFRTRRFYQSLVQRRLKD
Sbjct: 493 AFLGFLVAVVLFFRTRRFYQSLVQRRLKD 521

[Close]

BLAST of MC06g_new0033 vs. ExPASy TrEMBL
Match: A0A6J1EZJ6 (uncharacterized protein LOC111440723 OS=Cucurbita moschata OX=3662 GN=LOC111440723 PE=4 SV=1)

HSP 1 Score: 507 bits (1305), Expect = 4.71e-176
Identity = 271/332 (81.63%), Postives = 293/332 (88.25%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FVVI+KTE GNES HLNS S IALIIS YLTILIIL+N   LP W R+FTFVLLL L+
Sbjct: 184 MRFVVIEKTEIGNESTHLNSLSKIALIISCYLTILIILDNVFFLPTWVRLFTFVLLLILL 243

Query: 61  ASPLGIAIRARTEDSVF---LQNADDSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGTV 120
           ASPLGIA RA+TEDSV    LQ+ D+SVEY QIP E Q +DQL +V++GEMN+IEAIGTV
Sbjct: 244 ASPLGIANRAQTEDSVSKTKLQSTDNSVEYHQIPGEEQRNDQLMMVTNGEMNVIEAIGTV 303

Query: 121 NFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASD 180
           NFWLL  AMMCGMGSGLATINNMNQLGQSLGY TVEI+ FVSLWSIWNFLGRLG GYASD
Sbjct: 304 NFWLLFSAMMCGMGSGLATINNMNQLGQSLGYKTVEIHTFVSLWSIWNFLGRLGSGYASD 363

Query: 181 LLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAE 240
           LLLR+LG ARP LMA ALLTMS+GHIIIASGF GNLY+GSVIVGICYGSQWSLMPAITAE
Sbjct: 364 LLLRELGWARPLLMAVALLTMSVGHIIIASGFSGNLYLGSVIVGICYGSQWSLMPAITAE 423

Query: 241 IFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVM 300
           IFGIR+MGTI+NTITVASPI SY+LSVRVIGY YDREA  SAGDNSCSGKHCFMTSFL+M
Sbjct: 424 IFGIRHMGTIYNTITVASPIGSYVLSVRVIGYIYDREA--SAGDNSCSGKHCFMTSFLIM 483

Query: 301 AAVAFLGFLVAVVLFFRTRRFYQSLVQRRLKD 329
           AAVAFLGF+VAV LF RTRRFYQ LVQR+LKD
Sbjct: 484 AAVAFLGFIVAVALFLRTRRFYQLLVQRQLKD 513

[Close]

BLAST of MC06g_new0033 vs. ExPASy TrEMBL
Match: A0A5A7UT75 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002270 PE=4 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 7.50e-132
Identity = 216/349 (61.89%), Postives = 257/349 (73.64%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FV IDKTE+ NE  HLNS S +A+I++ YL ++IILNNA +L  W R FTF +LL L+
Sbjct: 197 MWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILL 256

Query: 61  ASPLGIAIRARTED------SVFLQ-----------NADDSVEYRQIPSEVQSDDQLAIV 120
           A+PLGIAI A+ ED      S+  +           +A+DSVEY ++P E   +DQ+ +V
Sbjct: 257 AAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEYHELPRE---EDQIMVV 316

Query: 121 SDGE----MNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVS 180
           S+      MN++EAI T+NFWLL  AM+CGMGSGLATINNM+QLGQSLGY   E   FVS
Sbjct: 317 SNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVS 376

Query: 181 LWSIWNFLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVI 240
           LWSIWNFLGR G GY SD L    G ARP LMA  LL MS GHI+IASGF GNLYVGS++
Sbjct: 377 LWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSIL 436

Query: 241 VGICYGSQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASA 300
           VGICYGSQWSLMP IT+EIFG+ +MGTIFNTI +ASP+ SYI SVRVIGY YDREA    
Sbjct: 437 VGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEH 496

Query: 301 GDNSCSGKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRLK 328
           G  +CSG HCF+ SFLVMA VAFLGFLVA  LFFRTRRFY+  +QRR++
Sbjct: 497 G--ACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

[Close]

BLAST of MC06g_new0033 vs. ExPASy TrEMBL
Match: A0A1S3BWC2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 7.50e-132
Identity = 216/349 (61.89%), Postives = 257/349 (73.64%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FV IDKTE+ NE  HLNS S +A+I++ YL ++IILNNA +L  W R FTF +LL L+
Sbjct: 197 MWFVRIDKTESKNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILL 256

Query: 61  ASPLGIAIRARTED------SVFLQ-----------NADDSVEYRQIPSEVQSDDQLAIV 120
           A+PLGIAI A+ ED      S+  +           +A+DSVEY ++P E   +DQ+ +V
Sbjct: 257 AAPLGIAINAQKEDFRGSPPSLITEESHVVNKPEPIDAEDSVEYHELPRE---EDQIMVV 316

Query: 121 SDGE----MNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVS 180
           S+      MN++EAI T+NFWLL  AM+CGMGSGLATINNM+QLGQSLGY   E   FVS
Sbjct: 317 SNSRAPQPMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTATETKTFVS 376

Query: 181 LWSIWNFLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVI 240
           LWSIWNFLGR G GY SD L    G ARP LMA  LL MS GHI+IASGF GNLYVGS++
Sbjct: 377 LWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSIL 436

Query: 241 VGICYGSQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASA 300
           VGICYGSQWSLMP IT+EIFG+ +MGTIFNTI +ASP+ SYI SVRVIGY YDREA    
Sbjct: 437 VGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAGEH 496

Query: 301 GDNSCSGKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRLK 328
           G  +CSG HCF+ SFLVMA VAFLGFLVA  LFFRTRRFY+  +QRR++
Sbjct: 497 G--ACSGTHCFVVSFLVMAIVAFLGFLVAAALFFRTRRFYRLALQRRVE 540

[Close]

BLAST of MC06g_new0033 vs. ExPASy TrEMBL
Match: A0A0A0LUE8 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G008470 PE=4 SV=1)

HSP 1 Score: 390 bits (1003), Expect = 6.89e-130
Identity = 215/348 (61.78%), Postives = 254/348 (72.99%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FV IDKTE+ NE  HLNS S +A+I++ YL ++IILNNA +L  W R FTF +LL L+
Sbjct: 197 MWFVRIDKTESSNEMKHLNSLSALAVIVAFYLMVVIILNNAFSLSSWTRYFTFSILLILL 256

Query: 61  ASPLGIAIRARTED------SVFLQ-----------NADDSVEYRQIPSEVQSDDQLAIV 120
           A+PLGIAI A+ ED      S+  +           +A+DSVEY ++P E   ++Q+ +V
Sbjct: 257 AAPLGIAINAQKEDFRGSSSSLIAEKSHVVNKPESIDAEDSVEYHELPRE---ENQIMVV 316

Query: 121 SDGE----MNIIEAIGTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVS 180
           S+      MN++EAI T+NFWLL  AM+CGMGSGLATINNM+QLGQSLGY   E   FVS
Sbjct: 317 SNTRAPQTMNVLEAIRTINFWLLFLAMVCGMGSGLATINNMSQLGQSLGYTETETKTFVS 376

Query: 181 LWSIWNFLGRLGPGYASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVI 240
           LWSIWNFLGR G GY SD L    G ARP LMA  LL MS GHI+IASGF GNLYVGS++
Sbjct: 377 LWSIWNFLGRFGAGYTSDFLFHTYGWARPLLMAITLLIMSGGHIVIASGFSGNLYVGSIL 436

Query: 241 VGICYGSQWSLMPAITAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASA 300
           VGICYGSQWSLMP IT+EIFG+ +MGTIFNTI +ASP+ SYI SVRVIGY YDREA    
Sbjct: 437 VGICYGSQWSLMPTITSEIFGLEHMGTIFNTIAIASPLGSYIFSVRVIGYIYDREAAREH 496

Query: 301 GDNSCSGKHCFMTSFLVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRL 327
           G  +CSG HCF+ SF VMA VAFLGFLVA  LFFRTRRFYQ   QRR+
Sbjct: 497 G--ACSGIHCFVVSFFVMAIVAFLGFLVAAALFFRTRRFYQLASQRRV 539

[Close]

BLAST of MC06g_new0033 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 4.4e-93
Identity = 190/333 (57.06%), Postives = 237/333 (71.17%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M  V I +T   ++  HLN  S ++LII+AYL I+IIL N   L  WA I T V LL ++
Sbjct: 200 MPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKNTFGLSSWANIVTLVCLLVML 259

Query: 61  ASPLGIAIRARTEDSVFLQNADDS---VEYRQIPSEVQSDDQLAIVSDG---EMNIIEAI 120
           A PL IA RA+ +        D S      +   S  QS +  + V  G    +N+++A+
Sbjct: 260 ALPLLIARRAQRDGMEKTVPHDYSPLISSPKATTSGNQSSEGDSKVEAGLSENLNLLQAM 319

Query: 121 GTVNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGY 180
             ++FWLL  AM+CGMGSGL+TINN+ Q+G+SL Y +VEIN+ VSLWSIWNFLGR G GY
Sbjct: 320 KKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGY 379

Query: 181 ASDLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAI 240
           ASD LL + G  RP LMAA L TMSIGH+IIASGF GNLYVGSVIVG+CYGSQWSLMP I
Sbjct: 380 ASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTI 439

Query: 241 TAEIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSF 300
           T+E+FGIR+MGTIFNTI+VASPI SYI SVR+IGY YD+   AS   N+C G HCF  SF
Sbjct: 440 TSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYIYDKT--ASGEGNTCYGSHCFRLSF 499

Query: 301 LVMAAVAFLGFLVAVVLFFRTRRFYQSLVQRRL 328
           ++MA+VAF GFLVA+VLFFRT+  Y+ ++ +RL
Sbjct: 500 IIMASVAFFGFLVAIVLFFRTKTLYRQILVKRL 530

[Close]

BLAST of MC06g_new0033 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-87
Identity = 172/330 (52.12%), Postives = 237/330 (71.82%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M  V + KT T +E  HL+  ST++LII+AYL I IIL + L+LP WA   T  +LL L+
Sbjct: 200 MPLVRVYKTSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLSLPSWANAVTLAVLLVLL 259

Query: 61  ASPLGIAIRARTEDSVFLQNADDSVEYRQIPSEVQ---SDDQLAIVSDGEMNIIEAIGTV 120
           +SPL +A+RA  +    ++    SV Y  +   ++   S + L +  D  +N+++A+  V
Sbjct: 260 SSPLLVAVRAHRDS---IEKPLSSV-YSPLVDNLEATTSGEILMLDEDKSLNLLQAMCNV 319

Query: 121 NFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASD 180
           +FWLL  AM+CGMGSG++TINN+ Q+G+SL Y +VEIN+ ++LW+IWNF+GR G GY SD
Sbjct: 320 DFWLLFLAMICGMGSGISTINNIRQIGESLRYTSVEINSLLALWNIWNFIGRFGGGYVSD 379

Query: 181 LLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAE 240
            LL + G  RP LMA  L TM+IGH+IIASGF GNLY GS+IVGICYGSQWSLMP IT+E
Sbjct: 380 WLLHRKGWPRPLLMATTLGTMTIGHLIIASGFQGNLYPGSIIVGICYGSQWSLMPTITSE 439

Query: 241 IFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVM 300
           +FG+++MGTI+NTI++ASP+ SYI SVR+IGY YDR        N+C G HCF  +++V+
Sbjct: 440 LFGVKHMGTIYNTISIASPMGSYIFSVRLIGYIYDRTIIGEG--NTCYGPHCFRLAYVVI 499

Query: 301 AAVAFLGFLVAVVLFFRTRRFYQSLVQRRL 328
           A+VAFLGFLV+ VL FRT+  Y+ + ++ L
Sbjct: 500 ASVAFLGFLVSCVLVFRTKTIYRQIFEKIL 523

[Close]

BLAST of MC06g_new0033 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 320.9 bits (821), Expect = 1.2e-87
Identity = 178/330 (53.94%), Postives = 233/330 (70.61%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FV + +T T ++  HL+  S I++II+AYL ++I + N L L    +IF+F+L+L L+
Sbjct: 194 MPFVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFSFILVLLLL 253

Query: 61  ASPLGIAIRARTEDSVFLQNAD----DSVEYRQIPSEVQSDDQLAIVSDGEMNIIEAIGT 120
           ASPL +A+RA  E    L + D    D+      PS     D   +V++ + NI+EA+ T
Sbjct: 254 ASPLLVAVRALREKRQTLSSLDGPVLDTSALLDPPSSNIFPDGDHLVAE-DSNILEAMST 313

Query: 121 VNFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYAS 180
           VNFWLL  AM+CGMGSG AT+NNM Q+G+SL Y +V++N+ VSLWSIWNFLGR G GY S
Sbjct: 314 VNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNSLVSLWSIWNFLGRFGAGYVS 373

Query: 181 DLLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITA 240
           D  L +    RP  MA  L  M+IGHII+ASG  G+LY GSV++G+ YGSQWSLMP IT+
Sbjct: 374 DTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAGSVLIGMAYGSQWSLMPTITS 433

Query: 241 EIFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLV 300
           EIFGIR+MGTI+ TI++A PI SYILSV+VIGY YD+   AS  DNSC G  CF TSF++
Sbjct: 434 EIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKV--ASEDDNSCFGSQCFRTSFMI 493

Query: 301 MAAVAFLGFLVAVVLFFRTRRFYQSLVQRR 327
           MA+VA  G LVA VLFFRT +FY++LV +R
Sbjct: 494 MASVALFGSLVASVLFFRTHKFYKNLVAKR 520

[Close]

BLAST of MC06g_new0033 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 315.5 bits (807), Expect = 5.2e-86
Identity = 172/330 (52.12%), Postives = 227/330 (68.79%), Query Frame = 0


Query: 1   MAFVVIDKTETGNESNHLNSFSTIALIISAYLTILIILNNALTLPIWARIFTFVLLLTLI 60
           M FV    T    +  HLN  S I+LII  YL ++I++ N + + +  +I +F  LL L+
Sbjct: 196 MPFVRTYDTVIAGDKKHLNGLSAISLIIVTYLMVVILVENIIGMSMPMKICSFTFLLLLL 255

Query: 61  ASPLGIAIRARTEDSVFLQNADDSVEYRQI---PSEVQSDDQLAIVSDGEMNIIEAIGTV 120
           ASPL +A+RA+ E+     + D  V  R       ++ S   +  V   +MN++EAI T 
Sbjct: 256 ASPLLVAVRAQREEEHRFLSLDFPVTERTTLLDSPKLNSSSDVKDVMTNDMNVLEAICTT 315

Query: 121 NFWLLSFAMMCGMGSGLATINNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASD 180
           NFWLL  AM+CGMGSGLATINN+ Q+G+SL Y TV++N+ VSLWSIWNFLGR G GY SD
Sbjct: 316 NFWLLFVAMICGMGSGLATINNIRQMGESLRYSTVQLNSLVSLWSIWNFLGRFGSGYISD 375

Query: 181 LLLRQLGLARPFLMAAALLTMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAE 240
             L   G  RP  MA  L  M+IGHI++ASG  G+LY+GS++VG+ YGSQWSLMP IT+E
Sbjct: 376 TYLHSHGWPRPVFMAITLGLMAIGHIVMASGLLGSLYIGSLLVGLAYGSQWSLMPTITSE 435

Query: 241 IFGIRNMGTIFNTITVASPIASYILSVRVIGYTYDREAGASAGDNSCSGKHCFMTSFLVM 300
           IFG+ +MGTIF TI++ASP+ SY  SV+VIGY YD+   AS  D+SC G HCF TSFL+M
Sbjct: 436 IFGVLHMGTIFYTISIASPVGSYFFSVKVIGYLYDKV--ASEDDHSCYGNHCFRTSFLIM 495

Query: 301 AAVAFLGFLVAVVLFFRTRRFYQSLVQRRL 328
           AA+A LG LVA+VL  RT++FY +LV +R+
Sbjct: 496 AAMALLGSLVALVLLLRTKKFYATLVAKRI 523

[Close]

BLAST of MC06g_new0033 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 5.6e-48
Identity = 103/263 (39.16%), Postives = 159/263 (60.46%), Query Frame = 0


Query: 82  DDSVEYRQIPSEVQSDDQLAIVSD-----GEMNIIEAIGTVNFWLLSFAMMCGMGSGLAT 141
           DD  E +++  +V++      V +      +  I++A+ +V+  +L  A +CG+G  L  
Sbjct: 303 DDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTA 362

Query: 142 INNMNQLGQSLGYGTVEINAFVSLWSIWNFLGRLGPGYASDLLLRQLGLARPFLMAAALL 201
           I+N+ Q+G SLGY    ++ FVSL SIWN+ GR+  G  S++ L +    RP ++   LL
Sbjct: 363 IDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLL 422

Query: 202 TMSIGHIIIASGFPGNLYVGSVIVGICYGSQWSLMPAITAEIFGIRNMGTIFNTITVASP 261
               GH++IA   PG LYV SVI+G C+G+QW L+ AI +EIFG++   T++N  +VASP
Sbjct: 423 LSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASP 482

Query: 262 IASYILSVRVIGYTYDREAG----------ASAGDNSCSGKHCFMTSFLVMAAVAFLGFL 321
           I SY+L+VRV GY YD EAG              D +C G  CF  SF+++AAV   G L
Sbjct: 483 IGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVL 542

Query: 322 VAVVLFFRTRRFYQSLVQRRLKD 330
           V++VL  RT++FY+S + ++ ++
Sbjct: 543 VSMVLVIRTKKFYKSDIYKKFRE 565

The following BLAST results are available for this feature:

BLAST of MC06g_new0033 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6CGU8	1.0e-09	28.88	Probable transporter MCH1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=28...	[more]
Q5AXV1	1.1e-05	24.27	Probable transporter mch1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / ...	[more]
A0LNN5	1.5e-05	26.17	L-lactate transporter OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) ...	[more]
Q4WVT3	4.4e-05	28.97	Probable transporter mch1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 ...	[more]
Q9P3K8	2.2e-04	26.85	Probable transporter mch1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A /...	[more]

BLAST of MC06g_new0033 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134701.1	3.27e-221	100.00	LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 [Momordica charantia]	[more]
XP_022933362.1	9.72e-176	81.63	uncharacterized protein LOC111440723 [Cucurbita moschata]	[more]
KAG6587515.1	1.04e-174	81.33	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_023530984.1	7.94e-172	80.42	uncharacterized protein LOC111793373 [Cucurbita pepo subsp. pepo]	[more]
KAG7021495.1	8.91e-159	80.36	Protein NUCLEAR FUSION DEFECTIVE 4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC06g_new0033 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BYJ5	1.59e-221	100.00	LOW QUALITY PROTEIN: uncharacterized protein LOC111006908 OS=Momordica charantia...	[more]
A0A6J1EZJ6	4.71e-176	81.63	uncharacterized protein LOC111440723 OS=Cucurbita moschata OX=3662 GN=LOC1114407...	[more]
A0A5A7UT75	7.50e-132	61.89	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BWC2	7.50e-132	61.89	protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103493962 PE=4 ...	[more]
A0A0A0LUE8	6.89e-130	61.78	Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G00847...	[more]

BLAST of MC06g_new0033 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74780.1	4.4e-93	57.06	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G18940.1	1.2e-87	52.12	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34355.1	1.2e-87	53.94	Major facilitator superfamily protein	[more]
AT2G34350.1	5.2e-86	52.12	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G39210.1	5.6e-48	39.16	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 16..312 e-value: 2.2E-8 score: 35.2
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 18..317
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 1..328
None	No IPR available	PANTHER	PTHR21576:SF22	F25A4.25 PROTEIN	coord: 1..328

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g_new0033.1	MC06g_new0033.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity