Homology
BLAST of MC06g2240 vs. ExPASy Swiss-Prot
Match:
O64495 (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 SV=1)
HSP 1 Score: 1051.2 bits (2717), Expect = 5.7e-306
Identity = 514/767 (67.01%), Postives = 626/767 (81.62%), Query Frame = 0
Query: 9 FLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTL----SAEED 68
FL +FL F S + QTY++QLHP T F SK WHLSFL++ + EE+
Sbjct: 7 FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66
Query: 69 SSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGLEE-G 128
SSRLLYSY +A+EGFAAQL+E+E E L+ P+VVAVR D ++QTTYS KFLGL+ G
Sbjct: 67 PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126
Query: 129 RQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRK 188
GVW KS GQ IIGVLDTGVWPESPSF D+ MP IP KW+G CQEG+ F+SS CNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186
Query: 189 LIGAKFFIKGHHVASSPPS--DIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAG 248
LIGA+FFI+GH VA+SP ++ +EY+S RDS GHGTHT+ST G+SV+ A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246
Query: 249 VAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAI 308
VA+GMAPGA IAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306
Query: 309 GSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGE 368
G+FRAM+ GISVICAAGNNGPI+SSVAN APW++TIGA TLDRRFPAVVRL+ G+ +YGE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366
Query: 369 SMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQI 428
S+YPG + A +E+EV+Y+TGG G E CL+GSLPRE++ GKMV+CDRGVNGRSEKG+
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426
Query: 429 VKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGT 488
VKE+GG MILAN E+NQEE+ +DVH+LPATLIG+ E+ LKAY+N T PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486
Query: 489 VIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFT 548
VIGRSRAP VA+FS+RGPSL+NPS LKPD+IAPGVNIIAAWPQNLGPTGLP D+RR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546
Query: 549 VMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVFA 608
VMSGTSM+CPHVSGI ALI S +P W+PAAIKSA+MTTAD+ D GK I DGNKPAGVFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606
Query: 609 MGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGFS 668
+GAGHVNP +A++PGLVY+I+P +Y+ +LC LG+T S+I ITH NVSC+ +L+ N GFS
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666
Query: 669 LNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQS 728
LNYPS++V+FK G T++ ++RR+TNVGSPNSIYSV V APEG++V V P+RLVFKHV+Q+
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726
Query: 729 LNYRVWFI-SEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWK 768
L+YRVWF+ +K R G+ SF +G LTWV+S N RVRSPI+VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773
BLAST of MC06g2240 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 650.2 bits (1676), Expect = 2.9e-185
Identity = 355/760 (46.71%), Postives = 480/760 (63.16%), Query Frame = 0
Query: 12 TLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLSAEEDSSSRLLY 71
T+ FL L + + +TY+I+++ S F + W+ S L +S S LLY
Sbjct: 12 TIITTFLFLLLHTTAKKTYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSLLY 71
Query: 72 SYSNAMEGFAAQLSETELE-YLQKLPDVVAVRADKKYEIQTTYSPKFLGLEEGRQGVWYK 131
+Y+ + GF+A L TE + L ++ + D Y + TT +P+FLGL GV
Sbjct: 72 TYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL-NSEFGVHDL 131
Query: 132 SAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRKLIGAKFF 191
+ IIGVLDTGVWPES SF D+ MP IP KW+G C+ G +F+S +CN+KLIGA+ F
Sbjct: 132 GSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSF 191
Query: 192 IKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPGA 251
KG +AS +E VSPRD GHGTHTS+TAAG++V AS G AG A+GMA A
Sbjct: 192 SKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRA 251
Query: 252 RIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHG 311
R+A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG P++ D+IAIG+F AM+ G
Sbjct: 252 RVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERG 311
Query: 312 ISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGESMYPGNKLM 371
+ V C+AGN+GP ++SVAN APW+ T+GA TLDR FPA L G+ + G S+Y G +
Sbjct: 312 VFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGM- 371
Query: 372 EATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQIVKESGGAEM 431
TK LE+VY G LCL GSL V GK+VVCDRGVN R EKG +V+++GG M
Sbjct: 372 -GTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 431
Query: 432 ILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGTVIGRSRAPS 491
I+AN + EE + D H+LPA +G + L+ Y+ + + P A + F GTV+ +P
Sbjct: 432 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 491
Query: 492 VARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFTVMSGTSMAC 551
VA FSSRGP+ P LKPDVI PGVNI+A W +GPTGL +D+RR+ F +MSGTSM+C
Sbjct: 492 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 551
Query: 552 PHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDG--NKPAGVFAMGAGHVN 611
PH+SG+A L+ + HP W+P+AIKSA+MTTA V D+ P+ D N + +A G+GHV+
Sbjct: 552 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 611
Query: 612 PTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIF-IITHMNVSCHEVLQMNRGFSLNYPSM 671
P +A+ PGLVYDI EY+ LC+L YT I I+ +V+C + + + LNYPS
Sbjct: 612 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPSF 671
Query: 672 SVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQSLNYRVW 731
SV+F G +R +TNVG+ +S+Y V V+ V + VKP +L FK V + Y V
Sbjct: 672 SVLF-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVT 731
Query: 732 FISEKG-REGRKVSFGEGYLTWVHSENGKYRVRSPIAVTW 767
F+S+KG K FG +TW N ++ VRSP+A +W
Sbjct: 732 FVSKKGVSMTNKAEFGS--ITW---SNPQHEVRSPVAFSW 751
BLAST of MC06g2240 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 645.6 bits (1664), Expect = 7.2e-184
Identity = 352/763 (46.13%), Postives = 485/763 (63.56%), Query Frame = 0
Query: 9 FLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLSAEEDSSSR 68
F L L F + +++S TY++ + + +S FD W+ S L S+
Sbjct: 11 FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLRSI-----SDSAE 70
Query: 69 LLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGLEEGRQGVW 128
LLY+Y NA+ GF+ +L++ E + L P V++V + +YE+ TT +P FLGL+E ++
Sbjct: 71 LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLF 130
Query: 129 YKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRKLIGAK 188
++ ++GVLDTGVWPES S+SD PIP W+G C+ G F +S+CNRKLIGA+
Sbjct: 131 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 190
Query: 189 FFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAP 248
FF +G+ ++ P D +E SPRD GHGTHTSSTAAG+ V GAS+ G +G A+GMAP
Sbjct: 191 FFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250
Query: 249 GARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQ 308
AR+AVYKVCW GC+SSDI+AA+D AI D V++LS+SLGG ++ D +AIG+F AM+
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310
Query: 309 HGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGESMYPGNK 368
GI V C+AGN GP SS++N APWITT+GA TLDR FPA+ L G+ G S++ G
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370
Query: 369 LMEATKELEVVYL--TGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQIVKESG 428
L + K L +Y G LC+ G+L EKV+GK+V+CDRG+N R +KG +VK +G
Sbjct: 371 LPD--KLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 430
Query: 429 GAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGTVIGRS 488
G MILAN N EE + D H+LPAT +G + ++ Y+ T NP A I GTV+G
Sbjct: 431 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 490
Query: 489 RAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFTVMSGT 548
+P VA FSSRGP+ P+ LKPD+IAPGVNI+AAW GPTGL D+RR F ++SGT
Sbjct: 491 PSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGT 550
Query: 549 SMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILD--GNKPAGVFAMGA 608
SM+CPHVSG+AAL+ SVHP W+PAAI+SA+MTTA + GKP+LD KP+ F GA
Sbjct: 551 SMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGA 610
Query: 609 GHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGFSLNY 668
GHV+PT A +PGL+YD+ +Y+ LCAL YT +I ++ N +C + LNY
Sbjct: 611 GHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVA-DLNY 670
Query: 669 PSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEV-SAPEGVRVRVKPRRLVFKHVNQSLN 728
PS +V G + +R +T+VG + YSV+V S GV++ V+P L FK N+ +
Sbjct: 671 PSFAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKEANEKKS 730
Query: 729 YRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTW 767
Y V F + + SFG + W +GK+ V SP+A++W
Sbjct: 731 YTVTFTVDSSKPSGSNSFGS--IEW---SDGKHVVGSPVAISW 756
BLAST of MC06g2240 vs. ExPASy Swiss-Prot
Match:
Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)
HSP 1 Score: 628.6 bits (1620), Expect = 9.1e-179
Identity = 341/755 (45.17%), Postives = 472/755 (62.52%), Query Frame = 0
Query: 25 STLQTYLIQLHPQGITTSLFDSKLQWHLSFL----EQTLSAEEDSSSRLLYSYSNAMEGF 84
ST +TY+I + + + + LQW+ S + + EE +++R+LY+Y A G
Sbjct: 32 STKKTYVIHMDKSAMPLP-YTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGL 91
Query: 85 AAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGLE-EGRQGVWYKSAMGQRAII 144
AAQL++ E E L++ VVAV + +YE+ TT SP FLGLE + + VW + ++
Sbjct: 92 AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVV 151
Query: 145 GVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRKLIGAKFFIKGHHVASS 204
GVLDTG+WPES SF+D+ M P+P WRGAC+ G+ F CNRK++GA+ F +G+ A+
Sbjct: 152 GVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATG 211
Query: 205 PPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPGARIAVYKVCW 264
D EY SPRD GHGTHT++T AG+ V GA++FG G A+GMA AR+A YKVCW
Sbjct: 212 -KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCW 271
Query: 265 FSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVICAAGN 324
GC+SSDI++A+D A+ DGV +LS+SLGG + DS++I +F AM+ G+ V C+AGN
Sbjct: 272 VGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGN 331
Query: 325 NGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGESMYPGNKLMEATKELEVV 384
GP S+ N +PWITT+GAST+DR FPA V++ T G S+Y G ++ K+ +V
Sbjct: 332 GGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLV 391
Query: 385 YLTGGQMGGE---LCLKGSLPREKVEGKMVVCDRGVNGRSEKGQIVKESGGAEMILANAE 444
YL + CL G+L R V GK+V+CDRGV R +KGQ+VK +GG M+L N
Sbjct: 392 YLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTA 451
Query: 445 VNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGTVIGRSRAPSVARFSS 504
N EE + D H+LPA +G E +K Y T+ A ++ GT IG +P VA FSS
Sbjct: 452 TNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSS 511
Query: 505 RGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFTVMSGTSMACPHVSGI 564
RGP+ + LKPD++APGVNI+AAW ++ P+ L D RR F ++SGTSM+CPHVSG+
Sbjct: 512 RGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGV 571
Query: 565 AALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILD--GNKPAGVFAMGAGHVNPTRAVD 624
AALI S HP W+PAAIKSA+MTTA V D+ KP+ D G P+ + GAGH++P RA D
Sbjct: 572 AALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATD 631
Query: 625 PGLVYDIKPYEYVIHLCALGYTHSEIFIIT-HMNVSCHEVLQMNRGFSLNYPSMSVVFKH 684
PGLVYDI P EY LC + S++ + T H N +C L N G +LNYP++S +F
Sbjct: 632 PGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPG-NLNYPAISALFPE 691
Query: 685 GTTSK--TVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQSLNYRVWFISE 744
T K T+ R +TNVG S Y V VS +G V V+P+ L F +Q L+Y V F +
Sbjct: 692 NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTR 751
Query: 745 KGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTW 767
++ FG L W ++ ++VRSP+ +TW
Sbjct: 752 --FRMKRPEFGG--LVW---KSTTHKVRSPVIITW 776
BLAST of MC06g2240 vs. ExPASy Swiss-Prot
Match:
Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)
HSP 1 Score: 624.8 bits (1610), Expect = 1.3e-177
Identity = 351/780 (45.00%), Postives = 477/780 (61.15%), Query Frame = 0
Query: 2 DSNTQMVFLSTLFLCFLSLRAN--ASTLQTYLIQ---LHPQGITTSLFDSKLQWHLSFLE 61
D+ M F S + FL A+ +S QTY+I + I TSLF+ S
Sbjct: 14 DNRPMMFFRSFIVFFFLIFFASNVSSRKQTYVIHTVTTSTKHIVTSLFN-------SLQT 73
Query: 62 QTLSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPK 121
+ ++ ++ S + Y Y NAM GF+A L++ +L+ ++ ++ D+ + TTYS +
Sbjct: 74 ENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHE 133
Query: 122 FLGLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFN 181
FLGLE G G+W ++++ IIG++DTG+ PE SF D+ M P+P +WRG+C EG F+
Sbjct: 134 FLGLEFG-IGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFS 193
Query: 182 SSICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVF 241
SS CN+K+IGA F KG+ ++ ++ S RD+ GHGTHT+STAAG V A+ F
Sbjct: 194 SSECNKKIIGASAFYKGYESIVGKINE-TTDFRSTRDAQGHGTHTASTAAGDIVPKANYF 253
Query: 242 GNGAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFD 301
G G+A GM +RIA YK CW GC S+D++AA+D AI DGVD++SLSLGG PF+
Sbjct: 254 GQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYV 313
Query: 302 DSIAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGE 361
D IAI F AMQ I V C+AGN+GP S+V+N APW+ T+ AS DR FPA+VR+ +
Sbjct: 314 DPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRK 373
Query: 362 AIYGESMYPGNKLMEATKELEVVY--LTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNG 421
++ G S+Y G L K L + + G + G C++ SL RE VEGK+V+C RG +G
Sbjct: 374 SLVGSSLYKGKSL----KNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASG 433
Query: 422 RSEKGQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKA 481
R+ KG+ VK SGGA M+L + E EE L D HVLPA +GF++ L Y+ N A
Sbjct: 434 RTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATA 493
Query: 482 RIQFGGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPED 541
++F GT G + AP VA FSSRGPS++ P KPD+ APG+NI+A W P+ L D
Sbjct: 494 SVRFRGTAYG-ATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSD 553
Query: 542 TRRSNFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILD-- 601
RR F ++SGTSMACPH+SGIAALI SVH W+PA IKSAIMTTA ++D+ +PI D
Sbjct: 554 PRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRG 613
Query: 602 ---GNKPAGVFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVS 661
A FA GAG+V+PTRAVDPGLVYD +Y+ +LC+L YT I + + N +
Sbjct: 614 AAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYT 673
Query: 662 CHEVLQMNRGFSLNYPSMSVVFKHGTTSKTV--SRRLTNVGSPNSIYSVEVSAPEGVRVR 721
C + LNYPS +V +G KTV R +TNVGSP Y V V P+GV+VR
Sbjct: 674 CASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVR 733
Query: 722 VKPRRLVFKHVNQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWK 768
V+P+ L F+ + L+Y V + +E R SF G L W+ KY VRSPIAVTW+
Sbjct: 734 VEPKVLKFQKARERLSYTVTYDAEASRNSSSSSF--GVLVWICD---KYNVRSPIAVTWE 774
BLAST of MC06g2240 vs. NCBI nr
Match:
XP_022155682.1 (subtilisin-like protease SBT1.2 [Momordica charantia])
HSP 1 Score: 1531 bits (3965), Expect = 0.0
Identity = 767/768 (99.87%), Postives = 767/768 (99.87%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLS 60
MDSNTQMVFLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLS
Sbjct: 1 MDSNTQMVFLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLS 60
Query: 61 AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGL 120
AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGL
Sbjct: 61 AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGL 120
Query: 121 EEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSIC 180
EEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSIC
Sbjct: 121 EEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSIC 180
Query: 181 NRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGA 240
NRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGA 240
Query: 241 GVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA 300
GVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA 300
Query: 301 IGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYG 360
IGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYG
Sbjct: 301 IGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYG 360
Query: 361 ESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQ 420
ESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQ 420
Query: 421 IVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGG 480
IVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGG
Sbjct: 421 IVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGG 480
Query: 481 TVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNF 540
TVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNF
Sbjct: 481 TVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNF 540
Query: 541 TVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVF 600
TVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVF
Sbjct: 541 TVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVF 600
Query: 601 AMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGF 660
AMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGF
Sbjct: 601 AMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGF 660
Query: 661 SLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQ 720
SLNYPSMSVVFKHGTTSKTVSRR TNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQ
Sbjct: 661 SLNYPSMSVVFKHGTTSKTVSRRXTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQ 720
Query: 721 SLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
SLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN
Sbjct: 721 SLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
BLAST of MC06g2240 vs. NCBI nr
Match:
XP_022995265.1 (subtilisin-like protease SBT1.2 [Cucurbita maxima])
HSP 1 Score: 1392 bits (3602), Expect = 0.0
Identity = 688/770 (89.35%), Postives = 734/770 (95.32%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSLR--ANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD N+QM+ LSTLFLCFLSL+ AN STLQTY+IQLHPQG+T+S F SK QWHLSFLEQT
Sbjct: 1 MDFNSQMLLLSTLFLCFLSLQVQANTSTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LSAEEDSSSRLLYSYSNAMEGFAAQLSETE+EYL+KLPDVVAVRADKKYEIQTTYSPKFL
Sbjct: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADKKYEIQTTYSPKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL G QGVW+ SAMG+ AI+GVLDTGVWPESPSFSDS+MP IP KWRGACQEGQ+FNSS
Sbjct: 121 GLSLGSQGVWFNSAMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CN+KLIGAKFFIKGHHVASSPPSD+ QEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAP A IAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV CAAGNNGPIQSSVAN APWITTIGASTLDRRFPA+VRLS GE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK ATKELEVVYLTGGQMGGELCLKGSLPRE+VEGK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN E+N EE+LVDVHVLPATLIGF+EANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFSEANRLKAYMNTTSNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPED+RRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAG 600
NFTVMSGTSMACPHVSGIAALIHS HP+WTPAAIKSAIMTTA+V+DH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
Query: 601 VFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNR 660
VFAMGAGH+NPT+A+DPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCH+VLQMN+
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
Query: 661 GFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHV 720
GFSLNYPSMSVVFKH TTSK VSRRLTNVGSPNSIYSVEV+AP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
Query: 721 NQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
NQSLNY+VWF+SEKG+EGRKVS EG+LTWVHSENGKY+VRSPI VTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
BLAST of MC06g2240 vs. NCBI nr
Match:
XP_038887438.1 (subtilisin-like protease SBT1.2 [Benincasa hispida])
HSP 1 Score: 1385 bits (3586), Expect = 0.0
Identity = 683/771 (88.59%), Postives = 731/771 (94.81%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSL--RANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD NTQ+ FL LFLCFLSL ++N + LQTY+IQLHP G+TTSL+DSKLQWHLSFLE+T
Sbjct: 1 MDFNTQLGFL--LFLCFLSLHVQSNTTALQTYIIQLHPHGLTTSLYDSKLQWHLSFLERT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LS EEDSSSRLLYSYSNAMEGFAAQLSETELEYL+KLPDVVAVRAD+KY+IQTTYS KFL
Sbjct: 61 LSVEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYQIQTTYSHKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL GRQGVW KS+MGQ AI+GVLDTGVWPESPSFSD KMPPIP KWRGACQEGQ+FNSS
Sbjct: 121 GLSVGRQGVWQKSSMGQGAIVGVLDTGVWPESPSFSDYKMPPIPPKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CNRKLIGAKFFIKGHHVASSPPSD+VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSPPSDVVQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAPGA IAVYKVCWFSGCYSSDIVAAMD+AI+DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIKDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV+CAAGNNGP QSSVAN APWITTIGA TLDRRFPA+V+LS GE I
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVQLSNGETI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK +ATKELEVVYLTGGQMGGELCLKGSLPRE+V+GKMVVCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREQVQGKMVVCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN+E+N EE+LVDVHVLPATLIGFAEANRLK+YINTT NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKSYINTTTNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLP+D+R S
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPDDSRSS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGN-KPA 600
NFTVMSGTSMACPHVSGIAALIHSVHP WTPAAIKSAIMTTADV+DHFGKPILDGN KPA
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSVHPNWTPAAIKSAIMTTADVTDHFGKPILDGNNKPA 600
Query: 601 GVFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMN 660
GVFAMGAGHVNPT+A+DPGL+YDIKPYEY+IHLCALGYTHSEIFI+THMNVSCH+V+QMN
Sbjct: 601 GVFAMGAGHVNPTKAIDPGLIYDIKPYEYIIHLCALGYTHSEIFIVTHMNVSCHKVVQMN 660
Query: 661 RGFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKH 720
+GF+LNYPSMSV+FKHGTTSK VSRRLTNVG+PNSIY VEV+APEGVRVRVKPRRLVFKH
Sbjct: 661 KGFTLNYPSMSVIFKHGTTSKMVSRRLTNVGTPNSIYKVEVAAPEGVRVRVKPRRLVFKH 720
Query: 721 VNQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
VNQSLNY+VWF SEKG+EGRKVS EG+LTW+HSEN KY+VRSPI VTWKN
Sbjct: 721 VNQSLNYKVWFTSEKGKEGRKVSSTEGHLTWLHSENSKYKVRSPIVVTWKN 769
BLAST of MC06g2240 vs. NCBI nr
Match:
XP_023532819.1 (subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 684/770 (88.83%), Postives = 732/770 (95.06%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSLR--ANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD N+QM+ LSTLFLCFLSL+ A+ STLQTYLIQLHPQG+T+S F SK QWHLSFLEQT
Sbjct: 1 MDFNSQMLLLSTLFLCFLSLQVQADTSTLQTYLIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYL+KLPDVVAVRAD+KYEIQTTYSPKFL
Sbjct: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL G QGVW+ S+MG+ AI+GVLDTGVWPESPSFSDS+MPPIP KWRGACQEGQ+FNSS
Sbjct: 121 GLSLGTQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CN+KLIGAKFFIKGHHVASS PSD+ QEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAP A IAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV CAAGNNGPIQSSVAN APWITTIGASTLDRRFPA+VRL GE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLRNGEFI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK ATKELEVVYLTGGQMGGELCLKGSLPRE+VEGK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN E+N EE+LVDVHVLPATLIGFAEANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPED+RRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAG 600
NFTVMSGTSMACPHVSGIAALIHS HP+WT AAIKSAIMTTA+V+DH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTAAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
Query: 601 VFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNR 660
VFAMGAGH+NPT+A+DPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCH+VLQMN+
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
Query: 661 GFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHV 720
GFSLNYPSMSVVFKH TTSK +SRRLTNVGSPNSIYSVEV+AP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAISRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
Query: 721 NQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
NQSLNY+VWF+SEKG+EGRKVS EG+LTWVHSENGKY+VRSP+ VTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPMVVTWKN 770
BLAST of MC06g2240 vs. NCBI nr
Match:
KAG6606345.1 (Subtilisin-like protease 1.2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 684/770 (88.83%), Postives = 731/770 (94.94%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSLR--ANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD N+QM+ LSTLFLCFLSL+ AN TLQTY+IQLHPQG+T+S F SK QWHLSFLEQT
Sbjct: 1 MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LSAEEDSSSRLLYSYSNAMEGFAAQLSETE+EYL+KLPDVVAVRAD+KYEIQTTYSPKFL
Sbjct: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL G QGVW+ S+MG+ AI+GVLDTGVWPESPSFSDS+MPPIP KWRGACQEGQ+FNSS
Sbjct: 121 GLSLGSQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPPIPQKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CNRKLIGAKFFIKGHHVASS PSD+ QEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSQPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAP A IAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV CAAGNNGPIQSSVAN APWITTIGASTLDRRFPA+VRLS GE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK ATKELEVVYLTGGQMGGELCLKGSLPRE+VEGK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN +N EE+LVDVHVLPATLIGFAEANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANRLKAYMNTTSNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPED+RRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAG 600
NFTVMSGTSMACPHVSGIAALIHS HP+WTPAAIKSAIMTTA+V+DH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
Query: 601 VFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNR 660
VFAMGAGH+NPT+A+DPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCH+VLQMN+
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
Query: 661 GFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHV 720
GFSLNYPSMSVVFKH TTSK VSRRLTNVGSPNSIYSVEV+AP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
Query: 721 NQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
NQSLNY+VWF+SEKG+EG+ VS EG+LTWVHSENGKY+VRSPI VTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
BLAST of MC06g2240 vs. ExPASy TrEMBL
Match:
A0A6J1DQ04 (subtilisin-like protease SBT1.2 OS=Momordica charantia OX=3673 GN=LOC111022754 PE=3 SV=1)
HSP 1 Score: 1531 bits (3965), Expect = 0.0
Identity = 767/768 (99.87%), Postives = 767/768 (99.87%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLS 60
MDSNTQMVFLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLS
Sbjct: 1 MDSNTQMVFLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLS 60
Query: 61 AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGL 120
AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGL
Sbjct: 61 AEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGL 120
Query: 121 EEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSIC 180
EEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSIC
Sbjct: 121 EEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSIC 180
Query: 181 NRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGA 240
NRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGA
Sbjct: 181 NRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGA 240
Query: 241 GVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA 300
GVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA
Sbjct: 241 GVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIA 300
Query: 301 IGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYG 360
IGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYG
Sbjct: 301 IGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYG 360
Query: 361 ESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQ 420
ESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQ
Sbjct: 361 ESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQ 420
Query: 421 IVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGG 480
IVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGG
Sbjct: 421 IVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGG 480
Query: 481 TVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNF 540
TVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNF
Sbjct: 481 TVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNF 540
Query: 541 TVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVF 600
TVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVF
Sbjct: 541 TVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVF 600
Query: 601 AMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGF 660
AMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGF
Sbjct: 601 AMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGF 660
Query: 661 SLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQ 720
SLNYPSMSVVFKHGTTSKTVSRR TNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQ
Sbjct: 661 SLNYPSMSVVFKHGTTSKTVSRRXTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQ 720
Query: 721 SLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
SLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN
Sbjct: 721 SLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
BLAST of MC06g2240 vs. ExPASy TrEMBL
Match:
A0A6J1K3M4 (subtilisin-like protease SBT1.2 OS=Cucurbita maxima OX=3661 GN=LOC111490865 PE=3 SV=1)
HSP 1 Score: 1392 bits (3602), Expect = 0.0
Identity = 688/770 (89.35%), Postives = 734/770 (95.32%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSLR--ANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD N+QM+ LSTLFLCFLSL+ AN STLQTY+IQLHPQG+T+S F SK QWHLSFLEQT
Sbjct: 1 MDFNSQMLLLSTLFLCFLSLQVQANTSTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LSAEEDSSSRLLYSYSNAMEGFAAQLSETE+EYL+KLPDVVAVRADKKYEIQTTYSPKFL
Sbjct: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADKKYEIQTTYSPKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL G QGVW+ SAMG+ AI+GVLDTGVWPESPSFSDS+MP IP KWRGACQEGQ+FNSS
Sbjct: 121 GLSLGSQGVWFNSAMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CN+KLIGAKFFIKGHHVASSPPSD+ QEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAP A IAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV CAAGNNGPIQSSVAN APWITTIGASTLDRRFPA+VRLS GE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK ATKELEVVYLTGGQMGGELCLKGSLPRE+VEGK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN E+N EE+LVDVHVLPATLIGF+EANRLKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFSEANRLKAYMNTTSNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPED+RRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAG 600
NFTVMSGTSMACPHVSGIAALIHS HP+WTPAAIKSAIMTTA+V+DH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
Query: 601 VFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNR 660
VFAMGAGH+NPT+A+DPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCH+VLQMN+
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
Query: 661 GFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHV 720
GFSLNYPSMSVVFKH TTSK VSRRLTNVGSPNSIYSVEV+AP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
Query: 721 NQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
NQSLNY+VWF+SEKG+EGRKVS EG+LTWVHSENGKY+VRSPI VTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
BLAST of MC06g2240 vs. ExPASy TrEMBL
Match:
A0A6J1ES95 (subtilisin-like protease SBT1.2 OS=Cucurbita moschata OX=3662 GN=LOC111437268 PE=3 SV=1)
HSP 1 Score: 1381 bits (3574), Expect = 0.0
Identity = 682/770 (88.57%), Postives = 731/770 (94.94%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSLR--ANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD N+QM+ LSTLFLCFLSL+ AN TLQTY+IQLHPQG+T+S F SK QWHLSFLEQT
Sbjct: 1 MDFNSQMLLLSTLFLCFLSLQVQANIPTLQTYIIQLHPQGLTSSPFYSKRQWHLSFLEQT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LSAEEDSSSRLLYSYSNAMEGFAAQLSETE+EYL+KLPDVVAVRAD+KYEIQTTYSPKFL
Sbjct: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETEIEYLKKLPDVVAVRADRKYEIQTTYSPKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL G QGVW+ S+MG+ AI+GVLDTGVWPESPSFSDS+MP IP KWRGACQEGQ+FNSS
Sbjct: 121 GLSLGTQGVWFNSSMGEGAIVGVLDTGVWPESPSFSDSRMPSIPQKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CN+KLIGAKFFIKGHHVASSPPSD+ QEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNKKLIGAKFFIKGHHVASSPPSDVDQEYVSPRDSHGHGTHTSSTAAGASVAEASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAP A IAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPRAHIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV CAAGNNGPIQSSVAN APWITTIGASTLDRRFPA+VRLS GE I
Sbjct: 301 IAIGSFRAMQHGISVNCAAGNNGPIQSSVANVAPWITTIGASTLDRRFPAIVRLSNGEFI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK ATKELEVVYLTGGQMGGELCLKGSLPRE+VEGK+V+CDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKHATKELEVVYLTGGQMGGELCLKGSLPREQVEGKIVLCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN +N EE+LVDVHVLPATLIGFAEAN+LKAY+NTT+NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANTAINLEEDLVDVHVLPATLIGFAEANQLKAYMNTTSNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSL+NPSTLKPDVIAPGVNIIAAWPQNLGPTGLPED+RRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLTNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAG 600
NFTVMSGTSMACPHVSGIAALIHS HP+WTPAAIKSAIMTTA+V+DH+GKPILDG+KPAG
Sbjct: 541 NFTVMSGTSMACPHVSGIAALIHSAHPKWTPAAIKSAIMTTAEVTDHYGKPILDGSKPAG 600
Query: 601 VFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNR 660
VFAMGAGH+NPT+A+DPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCH+VLQMN+
Sbjct: 601 VFAMGAGHINPTKAIDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
Query: 661 GFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHV 720
GFSLNYPSMSVVFKH TTSK VSRRLTNVGSPNSIYSVEV+AP+GV+VRVKPRRLVFK+V
Sbjct: 661 GFSLNYPSMSVVFKHKTTSKAVSRRLTNVGSPNSIYSVEVTAPKGVQVRVKPRRLVFKYV 720
Query: 721 NQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
NQSLNY+VWF+SEKG+EG+ VS EG+LTWVHSENGKY+VRSPI VTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGKTVSSSEGHLTWVHSENGKYKVRSPIVVTWKN 770
BLAST of MC06g2240 vs. ExPASy TrEMBL
Match:
A0A5A7T534 (Subtilisin-like protease SBT1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold154G00500 PE=3 SV=1)
HSP 1 Score: 1376 bits (3561), Expect = 0.0
Identity = 683/770 (88.70%), Postives = 723/770 (93.90%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSL--RANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD NT+M FL LFL FLSL + N STLQTY+IQLHP G+ TS+FDSKLQWHLSFLEQT
Sbjct: 1 MDFNTRMGFL--LFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LSAEEDSSSRLLYSYSNAMEGFAAQL+ETELEYL+KLPDVVAVR D+KY+IQTTYS KFL
Sbjct: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL G QGVW KS+MGQ AI+G+LDTGVWPESPSFSDSKMPPIP KWRGACQEGQ+FNSS
Sbjct: 121 GLSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CNRKLIGAKFFIKGHHVASS PSD+VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAPGA IAVYKVCWFSGCYSSDIVAAMD+AI DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV+CAAGNNGP QSSVAN APWITTIGA TLDRRFPA+VRLS GEAI
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK +ATKELEVVYLTGGQMGGELCLKGSLPREKV+GKMVVCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN+E+N EE+LVDVHVLPATLIGFAEANRLKAYINTT NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LPED+RRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAG 600
NFTVMSGTSMACPHVSGI ALIHS HP+WTPAAIKSAIMTTADV+DHFGK ILDGNKPA
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600
Query: 601 VFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNR 660
VFAMGAGHVNPT+A+DPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCH+VLQMN+
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
Query: 661 GFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHV 720
GF+LNYPSMSV+FKHGTTSK VSRRLTNVGS NSIY V+V+APEGVRVRVKPRRLVFKHV
Sbjct: 661 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720
Query: 721 NQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
NQSLNY+VWF+SEKG+EGRKV F EG LTW+H EN KY+VRSPI VTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768
BLAST of MC06g2240 vs. ExPASy TrEMBL
Match:
A0A1S3BHA2 (subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=3 SV=1)
HSP 1 Score: 1376 bits (3561), Expect = 0.0
Identity = 683/770 (88.70%), Postives = 723/770 (93.90%), Query Frame = 0
Query: 1 MDSNTQMVFLSTLFLCFLSL--RANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQT 60
MD NT+M FL LFL FLSL + N STLQTY+IQLHP G+ TS+FDSKLQWHLSFLEQT
Sbjct: 1 MDFNTRMGFL--LFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQT 60
Query: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFL 120
LSAEEDSSSRLLYSYSNAMEGFAAQL+ETELEYL+KLPDVVAVR D+KY+IQTTYS KFL
Sbjct: 61 LSAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFL 120
Query: 121 GLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSS 180
GL G QGVW KS+MGQ AI+G+LDTGVWPESPSFSDSKMPPIP KWRGACQEGQ+FNSS
Sbjct: 121 GLSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSS 180
Query: 181 ICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGN 240
CNRKLIGAKFFIKGHHVASS PSD+VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGN
Sbjct: 181 NCNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGN 240
Query: 241 GAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDS 300
GAGVAQGMAPGA IAVYKVCWFSGCYSSDIVAAMD+AI DGVDILSLSLGGFPLPFFDDS
Sbjct: 241 GAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDS 300
Query: 301 IAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAI 360
IAIGSFRAMQHGISV+CAAGNNGP QSSVAN APWITTIGA TLDRRFPA+VRLS GEAI
Sbjct: 301 IAIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAI 360
Query: 361 YGESMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEK 420
YGESMYPGNK +ATKELEVVYLTGGQMGGELCLKGSLPREKV+GKMVVCDRGVNGRSEK
Sbjct: 361 YGESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEK 420
Query: 421 GQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQF 480
GQIVKESGGA MILAN+E+N EE+LVDVHVLPATLIGFAEANRLKAYINTT NPKARIQF
Sbjct: 421 GQIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQF 480
Query: 481 GGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRS 540
GGTVIGRSRAPSVA+FSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPT LPED+RRS
Sbjct: 481 GGTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRS 540
Query: 541 NFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAG 600
NFTVMSGTSMACPHVSGI ALIHS HP+WTPAAIKSAIMTTADV+DHFGK ILDGNKPA
Sbjct: 541 NFTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPAD 600
Query: 601 VFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNR 660
VFAMGAGHVNPT+A+DPGLVYDIKPYEY+IHLCALGYTHSEIFIITHMNVSCH+VLQMN+
Sbjct: 601 VFAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNK 660
Query: 661 GFSLNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHV 720
GF+LNYPSMSV+FKHGTTSK VSRRLTNVGS NSIY V+V+APEGVRVRVKPRRLVFKHV
Sbjct: 661 GFTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHV 720
Query: 721 NQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWKN 768
NQSLNY+VWF+SEKG+EGRKV F EG LTW+H EN KY+VRSPI VTWKN
Sbjct: 721 NQSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768
BLAST of MC06g2240 vs. TAIR 10
Match:
AT1G04110.1 (Subtilase family protein )
HSP 1 Score: 1051.2 bits (2717), Expect = 4.0e-307
Identity = 514/767 (67.01%), Postives = 626/767 (81.62%), Query Frame = 0
Query: 9 FLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTL----SAEED 68
FL +FL F S + QTY++QLHP T F SK WHLSFL++ + EE+
Sbjct: 7 FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66
Query: 69 SSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGLEE-G 128
SSRLLYSY +A+EGFAAQL+E+E E L+ P+VVAVR D ++QTTYS KFLGL+ G
Sbjct: 67 PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126
Query: 129 RQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRK 188
GVW KS GQ IIGVLDTGVWPESPSF D+ MP IP KW+G CQEG+ F+SS CNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186
Query: 189 LIGAKFFIKGHHVASSPPS--DIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAG 248
LIGA+FFI+GH VA+SP ++ +EY+S RDS GHGTHT+ST G+SV+ A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246
Query: 249 VAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAI 308
VA+GMAPGA IAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD+IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306
Query: 309 GSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGE 368
G+FRAM+ GISVICAAGNNGPI+SSVAN APW++TIGA TLDRRFPAVVRL+ G+ +YGE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366
Query: 369 SMYPGNKLMEATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQI 428
S+YPG + A +E+EV+Y+TGG G E CL+GSLPRE++ GKMV+CDRGVNGRSEKG+
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426
Query: 429 VKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGT 488
VKE+GG MILAN E+NQEE+ +DVH+LPATLIG+ E+ LKAY+N T PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486
Query: 489 VIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFT 548
VIGRSRAP VA+FS+RGPSL+NPS LKPD+IAPGVNIIAAWPQNLGPTGLP D+RR NFT
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546
Query: 549 VMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDGNKPAGVFA 608
VMSGTSM+CPHVSGI ALI S +P W+PAAIKSA+MTTAD+ D GK I DGNKPAGVFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606
Query: 609 MGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGFS 668
+GAGHVNP +A++PGLVY+I+P +Y+ +LC LG+T S+I ITH NVSC+ +L+ N GFS
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666
Query: 669 LNYPSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQS 728
LNYPS++V+FK G T++ ++RR+TNVGSPNSIYSV V APEG++V V P+RLVFKHV+Q+
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726
Query: 729 LNYRVWFI-SEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWK 768
L+YRVWF+ +K R G+ SF +G LTWV+S N RVRSPI+VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773
BLAST of MC06g2240 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 650.2 bits (1676), Expect = 2.1e-186
Identity = 355/760 (46.71%), Postives = 480/760 (63.16%), Query Frame = 0
Query: 12 TLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLSAEEDSSSRLLY 71
T+ FL L + + +TY+I+++ S F + W+ S L +S S LLY
Sbjct: 12 TIITTFLFLLLHTTAKKTYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSLLY 71
Query: 72 SYSNAMEGFAAQLSETELE-YLQKLPDVVAVRADKKYEIQTTYSPKFLGLEEGRQGVWYK 131
+Y+ + GF+A L TE + L ++ + D Y + TT +P+FLGL GV
Sbjct: 72 TYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGL-NSEFGVHDL 131
Query: 132 SAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRKLIGAKFF 191
+ IIGVLDTGVWPES SF D+ MP IP KW+G C+ G +F+S +CN+KLIGA+ F
Sbjct: 132 GSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSF 191
Query: 192 IKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPGA 251
KG +AS +E VSPRD GHGTHTS+TAAG++V AS G AG A+GMA A
Sbjct: 192 SKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRA 251
Query: 252 RIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHG 311
R+A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG P++ D+IAIG+F AM+ G
Sbjct: 252 RVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERG 311
Query: 312 ISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGESMYPGNKLM 371
+ V C+AGN+GP ++SVAN APW+ T+GA TLDR FPA L G+ + G S+Y G +
Sbjct: 312 VFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGM- 371
Query: 372 EATKELEVVYLTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQIVKESGGAEM 431
TK LE+VY G LCL GSL V GK+VVCDRGVN R EKG +V+++GG M
Sbjct: 372 -GTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGM 431
Query: 432 ILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGTVIGRSRAPS 491
I+AN + EE + D H+LPA +G + L+ Y+ + + P A + F GTV+ +P
Sbjct: 432 IMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPV 491
Query: 492 VARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFTVMSGTSMAC 551
VA FSSRGP+ P LKPDVI PGVNI+A W +GPTGL +D+RR+ F +MSGTSM+C
Sbjct: 492 VAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSC 551
Query: 552 PHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILDG--NKPAGVFAMGAGHVN 611
PH+SG+A L+ + HP W+P+AIKSA+MTTA V D+ P+ D N + +A G+GHV+
Sbjct: 552 PHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVD 611
Query: 612 PTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIF-IITHMNVSCHEVLQMNRGFSLNYPSM 671
P +A+ PGLVYDI EY+ LC+L YT I I+ +V+C + + + LNYPS
Sbjct: 612 PQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPSF 671
Query: 672 SVVFKHGTTSKTVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQSLNYRVW 731
SV+F G +R +TNVG+ +S+Y V V+ V + VKP +L FK V + Y V
Sbjct: 672 SVLF-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVT 731
Query: 732 FISEKG-REGRKVSFGEGYLTWVHSENGKYRVRSPIAVTW 767
F+S+KG K FG +TW N ++ VRSP+A +W
Sbjct: 732 FVSKKGVSMTNKAEFGS--ITW---SNPQHEVRSPVAFSW 751
BLAST of MC06g2240 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 645.6 bits (1664), Expect = 5.1e-185
Identity = 352/763 (46.13%), Postives = 485/763 (63.56%), Query Frame = 0
Query: 9 FLSTLFLCFLSLRANASTLQTYLIQLHPQGITTSLFDSKLQWHLSFLEQTLSAEEDSSSR 68
F L L F + +++S TY++ + + +S FD W+ S L S+
Sbjct: 11 FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLRSI-----SDSAE 70
Query: 69 LLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGLEEGRQGVW 128
LLY+Y NA+ GF+ +L++ E + L P V++V + +YE+ TT +P FLGL+E ++
Sbjct: 71 LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLF 130
Query: 129 YKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRKLIGAK 188
++ ++GVLDTGVWPES S+SD PIP W+G C+ G F +S+CNRKLIGA+
Sbjct: 131 PEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGAR 190
Query: 189 FFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAP 248
FF +G+ ++ P D +E SPRD GHGTHTSSTAAG+ V GAS+ G +G A+GMAP
Sbjct: 191 FFARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 250
Query: 249 GARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQ 308
AR+AVYKVCW GC+SSDI+AA+D AI D V++LS+SLGG ++ D +AIG+F AM+
Sbjct: 251 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 310
Query: 309 HGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGESMYPGNK 368
GI V C+AGN GP SS++N APWITT+GA TLDR FPA+ L G+ G S++ G
Sbjct: 311 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 370
Query: 369 LMEATKELEVVYL--TGGQMGGELCLKGSLPREKVEGKMVVCDRGVNGRSEKGQIVKESG 428
L + K L +Y G LC+ G+L EKV+GK+V+CDRG+N R +KG +VK +G
Sbjct: 371 LPD--KLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 430
Query: 429 GAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGTVIGRS 488
G MILAN N EE + D H+LPAT +G + ++ Y+ T NP A I GTV+G
Sbjct: 431 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 490
Query: 489 RAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFTVMSGT 548
+P VA FSSRGP+ P+ LKPD+IAPGVNI+AAW GPTGL D+RR F ++SGT
Sbjct: 491 PSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGT 550
Query: 549 SMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILD--GNKPAGVFAMGA 608
SM+CPHVSG+AAL+ SVHP W+PAAI+SA+MTTA + GKP+LD KP+ F GA
Sbjct: 551 SMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGA 610
Query: 609 GHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVSCHEVLQMNRGFSLNY 668
GHV+PT A +PGL+YD+ +Y+ LCAL YT +I ++ N +C + LNY
Sbjct: 611 GHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVA-DLNY 670
Query: 669 PSMSVVFKHGTTSKTVSRRLTNVGSPNSIYSVEV-SAPEGVRVRVKPRRLVFKHVNQSLN 728
PS +V G + +R +T+VG + YSV+V S GV++ V+P L FK N+ +
Sbjct: 671 PSFAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKEANEKKS 730
Query: 729 YRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTW 767
Y V F + + SFG + W +GK+ V SP+A++W
Sbjct: 731 YTVTFTVDSSKPSGSNSFGS--IEW---SDGKHVVGSPVAISW 756
BLAST of MC06g2240 vs. TAIR 10
Match:
AT5G51750.1 (subtilase 1.3 )
HSP 1 Score: 628.6 bits (1620), Expect = 6.4e-180
Identity = 341/755 (45.17%), Postives = 472/755 (62.52%), Query Frame = 0
Query: 25 STLQTYLIQLHPQGITTSLFDSKLQWHLSFL----EQTLSAEEDSSSRLLYSYSNAMEGF 84
ST +TY+I + + + + LQW+ S + + EE +++R+LY+Y A G
Sbjct: 32 STKKTYVIHMDKSAMPLP-YTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGL 91
Query: 85 AAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPKFLGLE-EGRQGVWYKSAMGQRAII 144
AAQL++ E E L++ VVAV + +YE+ TT SP FLGLE + + VW + ++
Sbjct: 92 AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVV 151
Query: 145 GVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFNSSICNRKLIGAKFFIKGHHVASS 204
GVLDTG+WPES SF+D+ M P+P WRGAC+ G+ F CNRK++GA+ F +G+ A+
Sbjct: 152 GVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATG 211
Query: 205 PPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPGARIAVYKVCW 264
D EY SPRD GHGTHT++T AG+ V GA++FG G A+GMA AR+A YKVCW
Sbjct: 212 -KIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCW 271
Query: 265 FSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFDDSIAIGSFRAMQHGISVICAAGN 324
GC+SSDI++A+D A+ DGV +LS+SLGG + DS++I +F AM+ G+ V C+AGN
Sbjct: 272 VGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGN 331
Query: 325 NGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGEAIYGESMYPGNKLMEATKELEVV 384
GP S+ N +PWITT+GAST+DR FPA V++ T G S+Y G ++ K+ +V
Sbjct: 332 GGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLV 391
Query: 385 YLTGGQMGGE---LCLKGSLPREKVEGKMVVCDRGVNGRSEKGQIVKESGGAEMILANAE 444
YL + CL G+L R V GK+V+CDRGV R +KGQ+VK +GG M+L N
Sbjct: 392 YLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTA 451
Query: 445 VNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKARIQFGGTVIGRSRAPSVARFSS 504
N EE + D H+LPA +G E +K Y T+ A ++ GT IG +P VA FSS
Sbjct: 452 TNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSS 511
Query: 505 RGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDTRRSNFTVMSGTSMACPHVSGI 564
RGP+ + LKPD++APGVNI+AAW ++ P+ L D RR F ++SGTSM+CPHVSG+
Sbjct: 512 RGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGV 571
Query: 565 AALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILD--GNKPAGVFAMGAGHVNPTRAVD 624
AALI S HP W+PAAIKSA+MTTA V D+ KP+ D G P+ + GAGH++P RA D
Sbjct: 572 AALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATD 631
Query: 625 PGLVYDIKPYEYVIHLCALGYTHSEIFIIT-HMNVSCHEVLQMNRGFSLNYPSMSVVFKH 684
PGLVYDI P EY LC + S++ + T H N +C L N G +LNYP++S +F
Sbjct: 632 PGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPG-NLNYPAISALFPE 691
Query: 685 GTTSK--TVSRRLTNVGSPNSIYSVEVSAPEGVRVRVKPRRLVFKHVNQSLNYRVWFISE 744
T K T+ R +TNVG S Y V VS +G V V+P+ L F +Q L+Y V F +
Sbjct: 692 NTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTR 751
Query: 745 KGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTW 767
++ FG L W ++ ++VRSP+ +TW
Sbjct: 752 --FRMKRPEFGG--LVW---KSTTHKVRSPVIITW 776
BLAST of MC06g2240 vs. TAIR 10
Match:
AT1G01900.1 (subtilase family protein )
HSP 1 Score: 624.8 bits (1610), Expect = 9.3e-179
Identity = 351/780 (45.00%), Postives = 477/780 (61.15%), Query Frame = 0
Query: 2 DSNTQMVFLSTLFLCFLSLRAN--ASTLQTYLIQ---LHPQGITTSLFDSKLQWHLSFLE 61
D+ M F S + FL A+ +S QTY+I + I TSLF+ S
Sbjct: 14 DNRPMMFFRSFIVFFFLIFFASNVSSRKQTYVIHTVTTSTKHIVTSLFN-------SLQT 73
Query: 62 QTLSAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLQKLPDVVAVRADKKYEIQTTYSPK 121
+ ++ ++ S + Y Y NAM GF+A L++ +L+ ++ ++ D+ + TTYS +
Sbjct: 74 ENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHE 133
Query: 122 FLGLEEGRQGVWYKSAMGQRAIIGVLDTGVWPESPSFSDSKMPPIPIKWRGACQEGQEFN 181
FLGLE G G+W ++++ IIG++DTG+ PE SF D+ M P+P +WRG+C EG F+
Sbjct: 134 FLGLEFG-IGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFS 193
Query: 182 SSICNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVF 241
SS CN+K+IGA F KG+ ++ ++ S RD+ GHGTHT+STAAG V A+ F
Sbjct: 194 SSECNKKIIGASAFYKGYESIVGKINE-TTDFRSTRDAQGHGTHTASTAAGDIVPKANYF 253
Query: 242 GNGAGVAQGMAPGARIAVYKVCWFSGCYSSDIVAAMDAAIRDGVDILSLSLGGFPLPFFD 301
G G+A GM +RIA YK CW GC S+D++AA+D AI DGVD++SLSLGG PF+
Sbjct: 254 GQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYV 313
Query: 302 DSIAIGSFRAMQHGISVICAAGNNGPIQSSVANAAPWITTIGASTLDRRFPAVVRLSTGE 361
D IAI F AMQ I V C+AGN+GP S+V+N APW+ T+ AS DR FPA+VR+ +
Sbjct: 314 DPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRK 373
Query: 362 AIYGESMYPGNKLMEATKELEVVY--LTGGQMGGELCLKGSLPREKVEGKMVVCDRGVNG 421
++ G S+Y G L K L + + G + G C++ SL RE VEGK+V+C RG +G
Sbjct: 374 SLVGSSLYKGKSL----KNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGASG 433
Query: 422 RSEKGQIVKESGGAEMILANAEVNQEENLVDVHVLPATLIGFAEANRLKAYINTTNNPKA 481
R+ KG+ VK SGGA M+L + E EE L D HVLPA +GF++ L Y+ N A
Sbjct: 434 RTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATA 493
Query: 482 RIQFGGTVIGRSRAPSVARFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPED 541
++F GT G + AP VA FSSRGPS++ P KPD+ APG+NI+A W P+ L D
Sbjct: 494 SVRFRGTAYG-ATAPMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSD 553
Query: 542 TRRSNFTVMSGTSMACPHVSGIAALIHSVHPRWTPAAIKSAIMTTADVSDHFGKPILD-- 601
RR F ++SGTSMACPH+SGIAALI SVH W+PA IKSAIMTTA ++D+ +PI D
Sbjct: 554 PRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRG 613
Query: 602 ---GNKPAGVFAMGAGHVNPTRAVDPGLVYDIKPYEYVIHLCALGYTHSEIFIITHMNVS 661
A FA GAG+V+PTRAVDPGLVYD +Y+ +LC+L YT I + + N +
Sbjct: 614 AAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYT 673
Query: 662 CHEVLQMNRGFSLNYPSMSVVFKHGTTSKTV--SRRLTNVGSPNSIYSVEVSAPEGVRVR 721
C + LNYPS +V +G KTV R +TNVGSP Y V V P+GV+VR
Sbjct: 674 CASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVR 733
Query: 722 VKPRRLVFKHVNQSLNYRVWFISEKGREGRKVSFGEGYLTWVHSENGKYRVRSPIAVTWK 768
V+P+ L F+ + L+Y V + +E R SF G L W+ KY VRSPIAVTW+
Sbjct: 734 VEPKVLKFQKARERLSYTVTYDAEASRNSSSSSF--GVLVWICD---KYNVRSPIAVTWE 774
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O64495 | 5.7e-306 | 67.01 | Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 S... | [more] |
| Q9ZUF6 | 2.9e-185 | 46.71 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
| O65351 | 7.2e-184 | 46.13 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9FLI4 | 9.1e-179 | 45.17 | Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... | [more] |
| Q84WS0 | 1.3e-177 | 45.00 | Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DQ04 | 0.0 | 99.87 | subtilisin-like protease SBT1.2 OS=Momordica charantia OX=3673 GN=LOC111022754 P... | [more] |
| A0A6J1K3M4 | 0.0 | 89.35 | subtilisin-like protease SBT1.2 OS=Cucurbita maxima OX=3661 GN=LOC111490865 PE=3... | [more] |
| A0A6J1ES95 | 0.0 | 88.57 | subtilisin-like protease SBT1.2 OS=Cucurbita moschata OX=3662 GN=LOC111437268 PE... | [more] |
| A0A5A7T534 | 0.0 | 88.70 | Subtilisin-like protease SBT1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A1S3BHA2 | 0.0 | 88.70 | subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=3 SV=... | [more] |
