MC06g2206 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g2206
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Pectinesterase
Location	MC06: 29431398 .. 29434023 (+)
RNA-Seq Expression	MC06g2206
Synteny	MC06g2206

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGGGCTTCTAAGAGGGAAGGTCCCCATCCTCCAAAAAAAAAAAAAAAAACGACCCTTTTTTTTTCTTTTATTCTTTCTTTCTCTCTTTTGATTATTTTCCCCCTCTCTTGGCCTCCTAGAGAGAGAGAGATCGTATTAGGAAGGAAACAAAAAAAAAAAAGAGCCACCTTTTTTCTTGTCCCAAAAGAAGGAAAAAGAGAAATTTAATTATTGTTTTTTAGAAAATGGGTTATAACGAAGATGATTCTGGAAATCGAAAGCGATACGCCATAATCGGCGTGTCGTCGATGTTGCTGGTGGCGATGGTGGCGGCGGTGACGGTGGGCGTAAACCTCAACCAAACCGAGACGAGCGATCCGGCCACCGGAAGCAAGTCCCACGAAATAACTACCTCCACAAAGGCCATTAAAACCATTTGTGCAACTGTGGATTACAAAGAAGACTGTATGAACAGCCTGAAGTCCGCCGGCCACAACTCGTCGGACCCGAAGGAGTTGATTCAGGCCGGCTTCAACGCCGCCGTGAAACTCCTCCAACAAGCCGCTGACAAGTCGGTCACCTTGAAGGAGATCGAGAAGGACCCAAGAACATCTCAAGCCCTTGCCGGCTGCAAGGAGCTCATGAACTTCGCCATCGACGAGCTTAAATACTCCTTCAAAAGGCTCGGTGACTTCGACATCACGAAACTCGACGAGATGCTCATCGACCTCAAGATCTGGCTCAGTGCCACCATTACTTACCAGGTACTCTTCACCCACCGCTCAACTCAAAGACTAAGCTGCAACTATTTCTCAGTAAATTTAATCTTTTATTTATGTTTCTTTACCAGGAAACTTGCTTGGATGCATTCGAGAACACAACCGGGAATGCGGCCGAGAAAATGAAGAAGGCGTTGAAGACGTCGATGCATTTGAGCAGCAACGGGCTGGCAATGGTGACGGAGATGTCGTCGATGCTGACGGACCTGCAGATTCCGGGCATCAGCCGCCGTCTCCTCGAGCTTCCAGTTGTGGTCGGGCACGACGACTACCCGGAGTGGGCCAGCCCCGGCTCACGACGGCTCCTTGATGCCTCTCCGGCGAGTATCAAGCCAAACGCGGTGGTGGCCAAGGACGGGAGCGGGAAGTACAGCACCATCCAGGATGCGCTGGACAAAGTTCCAAAGAAAAGGAAGGGCGTCTACGTGATTTACATCAAACAAGGTGTCTACAAGGAATACATTACCGTCAACAAAACTATGACCAAACTTATGTTCATCGGCGACGGCCCCACCAAGACCATTATCACCGGAAACAAGAACTTCGTCGACGGCATTCCCACCTTCAAAACTGCCACCGTTGGTGCGTTTCAATTTAGTCTTAATAATTTTAAAATGTTCCAGCAGGTGTATAGTCTAAAATACTGGCTGGATTTGTTTTGGATTTGTTTTCTTTCTCCTCTTATGATTTTGGTTGGTAGTGATTACTGCTGAAGACTTCATTGCACGAGACATCGGGATTGAGAACACAGCCGGGCCGGAGAAGCACCAGGCAGTGGCGTTGAGAGTCCAAGCCGACAAGGTGATATTCTATAACTGTGCCATGCATGCATACCAAGACACTCTCTACGTGCACACCATGAGGCAGTTCTACCGTGACTGCGTCGTGTCAGGCACCATCGACTTCGTCTTCGGCGATGCGGCTGCAGTCTTCCAAGGCTGCACGTTCCTCGTCCGCAAGCCGTTGCCGAACCAGCAGTGCATCGTAACAGCCCACGGGCGAAAGGAGCGCCGCCAGCCATCCGCGCTGATAATACAAAAATGCACCATCAAGGCCGACAAGGACCTAGAGCCAGTGAAGAAACAGTACAGATCCTTCTTGGGGCGACCGTGGAAGGAGTACTCAAGGACGCTCATCATGGACTCTTACATTGATAATGTGATCGATCCACAGGGGTGGCTGCCATGGAACGGGGACTGGGGCCTCAAGACATGTTTCTACAGCGAGGTTAACAACAACGGACCCGGGTCCGATAAGTCCAAGAGAGTGAAGTGGCGTGGAATTAAGAACATTACACCACAGCACGCCGCATCTTACACCGCTGGGCGGTTCTTGTTGGGAGATCGTTGGATCAAGCCCACCGGGATCCCCTACATCTCCGGCATGGTCAGCGGTGGCGCCGCTCATTGATCTCTTAATCTTCATCCTCCTGATCTGATTTTGATCTTTTTCCCGATCAGTGTTCTATGTCATGTAATGGCTCTCCCCCTTTTTTCCTCTCTCTCTCTCTCTTATGATTTCTATGTGTGGGTCTAATTCTATATAGTGGAAGCAAATATTTTATATTTATTCATTTGCAATCTTCCCAAGTTTTGGACCCCTTTTTTTTCTTCTTCTTCTTAATAAATAATCACCATTTAATTGATTCTTCATTAATTTCTTTTTAAATACATTTATAAAATATGTTCAATTACCAATTTCGTCACTCGTGGTCGTATCGTTGAGCTTCCCTTTCATTTTGGTTCTTTATAATTCGGTTAAATCTCACCCATTGTTCTAATGGAATACATAGTAAAATCTGTCTCAGAAAAAAAAAAAAGAATGCATGGTAAAATGTATTTATTTTTTTTTTAATTTTAAGTTTAAG

mRNA sequence

GGGGGCTTCTAAGAGGGAAGGTCCCCATCCTCCAAAAAAAAAAAAAAAAACGACCCTTTTTTTTTCTTTTATTCTTTCTTTCTCTCTTTTGATTATTTTCCCCCTCTCTTGGCCTCCTAGAGAGAGAGAGATCGTATTAGGAAGGAAACAAAAAAAAAAAAGAGCCACCTTTTTTCTTGTCCCAAAAGAAGGAAAAAGAGAAATTTAATTATTGTTTTTTAGAAAATGGGTTATAACGAAGATGATTCTGGAAATCGAAAGCGATACGCCATAATCGGCGTGTCGTCGATGTTGCTGGTGGCGATGGTGGCGGCGGTGACGGTGGGCGTAAACCTCAACCAAACCGAGACGAGCGATCCGGCCACCGGAAGCAAGTCCCACGAAATAACTACCTCCACAAAGGCCATTAAAACCATTTGTGCAACTGTGGATTACAAAGAAGACTGTATGAACAGCCTGAAGTCCGCCGGCCACAACTCGTCGGACCCGAAGGAGTTGATTCAGGCCGGCTTCAACGCCGCCGTGAAACTCCTCCAACAAGCCGCTGACAAGTCGGTCACCTTGAAGGAGATCGAGAAGGACCCAAGAACATCTCAAGCCCTTGCCGGCTGCAAGGAGCTCATGAACTTCGCCATCGACGAGCTTAAATACTCCTTCAAAAGGCTCGGTGACTTCGACATCACGAAACTCGACGAGATGCTCATCGACCTCAAGATCTGGCTCAGTGCCACCATTACTTACCAGGAAACTTGCTTGGATGCATTCGAGAACACAACCGGGAATGCGGCCGAGAAAATGAAGAAGGCGTTGAAGACGTCGATGCATTTGAGCAGCAACGGGCTGGCAATGGTGACGGAGATGTCGTCGATGCTGACGGACCTGCAGATTCCGGGCATCAGCCGCCGTCTCCTCGAGCTTCCAGTTGTGGTCGGGCACGACGACTACCCGGAGTGGGCCAGCCCCGGCTCACGACGGCTCCTTGATGCCTCTCCGGCGAGTATCAAGCCAAACGCGGTGGTGGCCAAGGACGGGAGCGGGAAGTACAGCACCATCCAGGATGCGCTGGACAAAGTTCCAAAGAAAAGGAAGGGCGTCTACGTGATTTACATCAAACAAGGTGTCTACAAGGAATACATTACCGTCAACAAAACTATGACCAAACTTATGTTCATCGGCGACGGCCCCACCAAGACCATTATCACCGGAAACAAGAACTTCGTCGACGGCATTCCCACCTTCAAAACTGCCACCGTTGTGATTACTGCTGAAGACTTCATTGCACGAGACATCGGGATTGAGAACACAGCCGGGCCGGAGAAGCACCAGGCAGTGGCGTTGAGAGTCCAAGCCGACAAGGTGATATTCTATAACTGTGCCATGCATGCATACCAAGACACTCTCTACGTGCACACCATGAGGCAGTTCTACCGTGACTGCGTCGTGTCAGGCACCATCGACTTCGTCTTCGGCGATGCGGCTGCAGTCTTCCAAGGCTGCACGTTCCTCGTCCGCAAGCCGTTGCCGAACCAGCAGTGCATCGTAACAGCCCACGGGCGAAAGGAGCGCCGCCAGCCATCCGCGCTGATAATACAAAAATGCACCATCAAGGCCGACAAGGACCTAGAGCCAGTGAAGAAACAGTACAGATCCTTCTTGGGGCGACCGTGGAAGGAGTACTCAAGGACGCTCATCATGGACTCTTACATTGATAATGTGATCGATCCACAGGGGTGGCTGCCATGGAACGGGGACTGGGGCCTCAAGACATGTTTCTACAGCGAGGTTAACAACAACGGACCCGGGTCCGATAAGTCCAAGAGAGTGAAGTGGCGTGGAATTAAGAACATTACACCACAGCACGCCGCATCTTACACCGCTGGGCGGTTCTTGTTGGGAGATCGTTGGATCAAGCCCACCGGGATCCCCTACATCTCCGGCATGGTCAGCGGTGGCGCCGCTCATTGATCTCTTAATCTTCATCCTCCTGATCTGATTTTGATCTTTTTCCCGATCAGTGTTCTATGTCATGTAATGGCTCTCCCCCTTTTTTCCTCTCTCTCTCTCTCTTATGATTTCTATGTGTGGGTCTAATTCTATATAGTGGAAGCAAATATTTTATATTTATTCATTTGCAATCTTCCCAAGTTTTGGACCCCTTTTTTTTCTTCTTCTTCTTAATAAATAATCACCATTTAATTGATTCTTCATTAATTTCTTTTTAAATACATTTATAAAATATGTTCAATTACCAATTTCGTCACTCGTGGTCGTATCGTTGAGCTTCCCTTTCATTTTGGTTCTTTATAATTCGGTTAAATCTCACCCATTGTTCTAATGGAATACATAGTAAAATCTGTCTCAGAAAAAAAAAAAAGAATGCATGGTAAAATGTATTTATTTTTTTTTTAATTTTAAGTTTAAG

Coding sequence (CDS)

ATGGGTTATAACGAAGATGATTCTGGAAATCGAAAGCGATACGCCATAATCGGCGTGTCGTCGATGTTGCTGGTGGCGATGGTGGCGGCGGTGACGGTGGGCGTAAACCTCAACCAAACCGAGACGAGCGATCCGGCCACCGGAAGCAAGTCCCACGAAATAACTACCTCCACAAAGGCCATTAAAACCATTTGTGCAACTGTGGATTACAAAGAAGACTGTATGAACAGCCTGAAGTCCGCCGGCCACAACTCGTCGGACCCGAAGGAGTTGATTCAGGCCGGCTTCAACGCCGCCGTGAAACTCCTCCAACAAGCCGCTGACAAGTCGGTCACCTTGAAGGAGATCGAGAAGGACCCAAGAACATCTCAAGCCCTTGCCGGCTGCAAGGAGCTCATGAACTTCGCCATCGACGAGCTTAAATACTCCTTCAAAAGGCTCGGTGACTTCGACATCACGAAACTCGACGAGATGCTCATCGACCTCAAGATCTGGCTCAGTGCCACCATTACTTACCAGGAAACTTGCTTGGATGCATTCGAGAACACAACCGGGAATGCGGCCGAGAAAATGAAGAAGGCGTTGAAGACGTCGATGCATTTGAGCAGCAACGGGCTGGCAATGGTGACGGAGATGTCGTCGATGCTGACGGACCTGCAGATTCCGGGCATCAGCCGCCGTCTCCTCGAGCTTCCAGTTGTGGTCGGGCACGACGACTACCCGGAGTGGGCCAGCCCCGGCTCACGACGGCTCCTTGATGCCTCTCCGGCGAGTATCAAGCCAAACGCGGTGGTGGCCAAGGACGGGAGCGGGAAGTACAGCACCATCCAGGATGCGCTGGACAAAGTTCCAAAGAAAAGGAAGGGCGTCTACGTGATTTACATCAAACAAGGTGTCTACAAGGAATACATTACCGTCAACAAAACTATGACCAAACTTATGTTCATCGGCGACGGCCCCACCAAGACCATTATCACCGGAAACAAGAACTTCGTCGACGGCATTCCCACCTTCAAAACTGCCACCGTTGTGATTACTGCTGAAGACTTCATTGCACGAGACATCGGGATTGAGAACACAGCCGGGCCGGAGAAGCACCAGGCAGTGGCGTTGAGAGTCCAAGCCGACAAGGTGATATTCTATAACTGTGCCATGCATGCATACCAAGACACTCTCTACGTGCACACCATGAGGCAGTTCTACCGTGACTGCGTCGTGTCAGGCACCATCGACTTCGTCTTCGGCGATGCGGCTGCAGTCTTCCAAGGCTGCACGTTCCTCGTCCGCAAGCCGTTGCCGAACCAGCAGTGCATCGTAACAGCCCACGGGCGAAAGGAGCGCCGCCAGCCATCCGCGCTGATAATACAAAAATGCACCATCAAGGCCGACAAGGACCTAGAGCCAGTGAAGAAACAGTACAGATCCTTCTTGGGGCGACCGTGGAAGGAGTACTCAAGGACGCTCATCATGGACTCTTACATTGATAATGTGATCGATCCACAGGGGTGGCTGCCATGGAACGGGGACTGGGGCCTCAAGACATGTTTCTACAGCGAGGTTAACAACAACGGACCCGGGTCCGATAAGTCCAAGAGAGTGAAGTGGCGTGGAATTAAGAACATTACACCACAGCACGCCGCATCTTACACCGCTGGGCGGTTCTTGTTGGGAGATCGTTGGATCAAGCCCACCGGGATCCCCTACATCTCCGGCATGGTCAGCGGTGGCGCCGCTCATTGA

Protein sequence

MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKAIKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDPRTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRLLELPVVVGHDDYPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIKQGVYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIENTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLGRPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGIKNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMVSGGAAH

Homology

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BLAST of MC06g2206 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 1.0e-182
Identity = 308/578 (53.29%), Postives = 423/578 (73.18%), Query Frame = 0


Query: 2   GYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKAI 61
           GY+++D+  +KRY II +SS+LL++MV AVT+GV++N+++ +         EITTS KAI
Sbjct: 4   GYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAI 63

Query: 62  KTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDPR 121
           K +CA  DYKE C ++L+    ++SDP EL++  FNA +K +   A KS T+ E++KDPR
Sbjct: 64  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 123

Query: 122 TSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFE 181
              AL  CKELM++AI EL  SF+ LG F+  K+DE L+ L+IWLSATI++++TCLD F+
Sbjct: 124 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGI-SRRLLELPVVVGHDDY 241
            T GNA E +KKALKT++ L+ NGLAMVTEMS+ L  +QIP + SRRLL         ++
Sbjct: 184 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS-------QEF 243

Query: 242 PEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIKQGVY 301
           P W    +RRLL+A  + +KP+ VVA+DGSG+Y TI +AL+ VPKK+   +V++IK+G+Y
Sbjct: 244 PSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIY 303

Query: 302 KEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIENT 361
           KEY+ VN++MT L+FIGDGP KT+I+G+K++ DGI T+KTATV I  + FIA++I  ENT
Sbjct: 304 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 363

Query: 362 AGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 421
           AG  KHQAVA+RV AD+ IFYNC    YQDTLY H+ RQFYRDC +SGTIDF+FGDAAAV
Sbjct: 364 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 423

Query: 422 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLGRP 481
           FQ CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q CTI  + D   VK+Q +++LGRP
Sbjct: 424 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 483

Query: 482 WKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGIKN 541
           WKEYSRT+IM+++I + + P+GW PW G++GL T FYSEV N GPG+  +KRV W GIK 
Sbjct: 484 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 543

Query: 542 ITPQHAASYTAGRFLLGDRWIKPTGIPYISGMVSGGAA 579
           ++ +    +T  +++ GD WI   G+PYI G+ SG  +
Sbjct: 544 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGS 568

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BLAST of MC06g2206 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 630.2 bits (1624), Expect = 2.4e-179
Identity = 309/582 (53.09%), Postives = 415/582 (71.31%), Query Frame = 0


Query: 1   MGYNEDDSGNRK-RYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTK 60
           M Y  DD   RK RY +I +SS+LL++MV AVTVGV+LN+ +           E+  S K
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA----EVNASVK 60

Query: 61  AIKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKD 120
           A+K +CA  DY++ C ++L   G N++DP EL++  FN  +K +  AA KS T+ E++KD
Sbjct: 61  AVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKD 120

Query: 121 PRTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDA 180
            RT  AL  CKELM++A+DEL  SF+ LG F+   LDE LI+L+IWLSA I+++ETCL+ 
Sbjct: 121 SRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEG 180

Query: 181 FENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGI-SRRLLELPVVVGHD 240
           F+ T GNA E MKKALKT++ L+ NGLA+++EMS+ +  +QIPG+ SRRLL        +
Sbjct: 181 FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-------E 240

Query: 241 DYPEWASPGSRRLLDASPA--SIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIK 300
            +P W     R+LL A+ A   +KP+ VVA+DGSG+Y TI +AL  VPKKR   +V++IK
Sbjct: 241 GFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIK 300

Query: 301 QGVYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIG 360
            G+YKEY+ VNKTM+ L+FIGDGP KTII+GNKN+ DGI T++TATV I    FIA++IG
Sbjct: 301 AGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIG 360

Query: 361 IENTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGD 420
            ENTAG  KHQAVA+RVQ+D+ IF+NC    YQDTLY H+ RQF+RDC +SGTIDF+FGD
Sbjct: 361 FENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGD 420

Query: 421 AAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSF 480
           AAAVFQ CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q CTI  + D   VK+  +++
Sbjct: 421 AAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAY 480

Query: 481 LGRPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWR 540
           LGRPWKEYSRT+IM+++I + + PQGW PW GD+GLKT FYSEV N GPGS  + RV W 
Sbjct: 481 LGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWA 540

Query: 541 GIKNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMVSGGAA 579
           GIK ++ +    +T  +++ GD WI   G+PY +G+++G  A
Sbjct: 541 GIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPA 571

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BLAST of MC06g2206 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 3.5e-151
Identity = 270/571 (47.29%), Postives = 376/571 (65.85%), Query Frame = 0


Query: 6   DDSGNRKRYAIIGVSSMLLVAMVAAV--TVGVNLNQTETSDPATGSKSHEITTSTKAIKT 65
           D    +K+  I GV + LLV MV AV  T   N + +E   P       +I T+T A++ 
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADK-SVTL-KEIEKDPR 125
           +CA  DYKE C+NSL  A  +S+ P +LI+ GFN  ++ ++ +  K SV L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 TSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFE 185
           T  AL  C++LMN A D+LK        F I ++++ + DL++WLS +I YQ+TC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRL--LELPVVVGHDD 245
            T    ++ M+K  KTS  L+SNGLAM+T +S++L +  + G++  L      ++   D 
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG 243

Query: 246 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIKQGV 305
            P W  P +RRL+ A+   +K N VVA DGSG+Y TI +AL+ VPK  +  +VIYIKQGV
Sbjct: 244 IPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGV 303

Query: 306 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDG-IPTFKTATVVITAEDFIARDIGIE 365
           Y E + V K MT + FIGDGPTKT ITG+ N+  G + T+ TATV I  ++F A++IG E
Sbjct: 304 YNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFE 363

Query: 366 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 425
           NTAGPE HQAVALRV AD  +FYNC +  YQDTLYVH+ RQF+RDC VSGT+DF+FGD  
Sbjct: 364 NTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGI 423

Query: 426 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 485
            V Q C  +VRKP+ +Q C++TA GR ++R+ + L++Q C I  +    PVK   +++LG
Sbjct: 424 VVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLG 483

Query: 486 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 545
           RPWKE+SRT+IM + ID+VIDP GWLPWNGD+ L T +Y+E  NNGPGS++++RVKW GI
Sbjct: 484 RPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGI 543

Query: 546 KNITPQHAASYTAGRFLLGDRWIKPTGIPYI 570
           K ++P+ A  +T  RFL G+ WI P  +PY+
Sbjct: 544 KKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

[Close]

BLAST of MC06g2206 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 505.8 bits (1301), Expect = 6.7e-142
Identity = 259/570 (45.44%), Postives = 368/570 (64.56%), Query Frame = 0


Query: 7   DSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKAIKTICA 66
           D   +K++ + G  S  LV MV +V V        TS  +     + I  +TKA++ +CA
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  TVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADK-SVTLK-EIEKDPRTSQ 126
             D+K+ C+NSL  A  +S DP +LI+ GF   +K + ++ +K S  +K + +K+P    
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFENTT 186
           A   C++LM  AID+LK        F + +++  + DL++WLS +I +Q+TC+D+F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRLLEL--PVVVGHDDYPE 246
            N  + M K  KTS  LSSN LAMVT +S+++ +  + G++  L +    ++   D  P 
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPT 243

Query: 247 WASPGSRRLLDA---SPASIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIKQGV 306
           W  P +RRL+ A    P  +K NAVVA+DG+G++ TI DAL+ VPK  K  ++I+IK+G+
Sbjct: 244 WVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGI 303

Query: 307 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNF-VDGIPTFKTATVVITAEDFIARDIGIE 366
           YKE +TV K M  + FIGDGP KT+ITG+ NF +  + TF TAT+ I  + F A++IGIE
Sbjct: 304 YKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIE 363

Query: 367 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 426
           NTAGPE  QAVALRV AD  +F++C +  +QDTLYVH+ RQFYRDC VSGT+DF+FGDA 
Sbjct: 364 NTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAK 423

Query: 427 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 486
            + Q C  +VRKP   Q C+VTA GR   R+ + L++  C I  D    P+K   +++LG
Sbjct: 424 CILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLG 483

Query: 487 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 546
           RPWKE+SRT+IM + ID+VIDP GWLPW+GD+ LKT +Y+E  N GPGS++++RVKW GI
Sbjct: 484 RPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGI 543

Query: 547 KNITPQHAASYTAGRFLLGDRWIKPTGIPY 569
           K +TPQ A  YT  RFL GD WI  T +PY
Sbjct: 544 KKLTPQDALLYTGDRFLRGDTWIPQTQVPY 564

[Close]

BLAST of MC06g2206 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.3e-124
Identity = 269/701 (38.37%), Postives = 372/701 (53.07%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKAIKTICATVDYKE 72
           +Y ++GV++ L++AMV  V      + +   +         IT +T +I  ICA+ DYK+
Sbjct: 3   KYVLLGVTA-LIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSI--ICASTDYKQ 62

Query: 73  DCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKS-VTLK-EIEKDPRTSQALAGCK 132
           DC  SL +    S DP+ LI++ F+ A+  ++   D+  + LK   + D  T +AL  C+
Sbjct: 63  DCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 133 ELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFENTTGNAAEK 192
           ELM+ AID+L+ +  +   F  T+L + + DL +WLS +ITYQ+TC+D FE     AA  
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 193 MKKALKTSMHLSSNGLAMVTEMSSMLTDLQIP----------------GISR-------- 252
           M++ ++   HL+SNGLA+   +  +L   +IP                G SR        
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 253 --------------------------------------RLLE------------------ 312
                                                 R L+                  
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 313 -------------LPV-------------------------------------------- 372
                        LP                                             
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 373 ---VVGHDDYPEWASPGSRRLLDASPAS--IKPNAVVAKDGSGKYSTIQDALDKVPKKRK 432
              +    ++P W +P SRRLL   P +  IK N VVAKDGSGK  TI  AL  VP K  
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNT 422

Query: 433 GVYVIYIKQGVYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFV-DGIPTFKTATVVITA 492
             +VI+IK+GVYKE + V K M  +MF+GDGPTKT+ITG+  F+ D + T++TA+V +  
Sbjct: 423 KKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNG 482

Query: 493 EDFIARDIGIENTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVS 552
           + F+A+DIG ENTAG  +HQAVALRV AD  +F+NC M+ YQDTLYVHT RQFYR+C VS
Sbjct: 483 DYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVS 542

Query: 553 GTIDFVFGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLE 569
           GTIDFVFGDA AVFQ C F++R+P+ +QQCIVTA GRK+RR+ + ++I    I  D    
Sbjct: 543 GTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYL 602

[Close]

BLAST of MC06g2206 vs. NCBI nr
Match: XP_008450602.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >KAA0050920.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa] >TYK10268.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa])

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 458/581 (78.83%), Postives = 522/581 (89.85%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           MGY +DDS N+KRYAIIGVSSMLLVAMV AVTVGVNLNQ ETSDPATG+KSHEI++S KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK++C+ SLK+ G+NSSDP+EL+QAGF AA+KL+Q AA+KSV LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           RTS+ALAGCKELM+FAIDELKYS  +LG+FDITKLDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRR-LLELPVVVGHDD 240
           +NTTGNAAEKMKKALKTSM LSSNGLAMV++ SSMLT+LQIPGISRR LLE+PV+ GHDD
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVL-GHDD 240

Query: 241 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQG 300
           YP+WA+PG RRLL A+ + +KPN VVAKDGSG++ TIQ ALD+VPK K    YVI+IK G
Sbjct: 241 YPDWANPGMRRLL-AAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAG 300

Query: 301 VYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIE 360
           VY+EY+ + KT+T LM IGDGP KTIITGNKNFVDG PTFKTATV +TAE F+ARDIG E
Sbjct: 301 VYQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFE 360

Query: 361 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 420
           NTAGP+KHQAVALRVQADK +FYNC MH YQDTLYVHTMRQFYRDC VSGTIDF+FGDAA
Sbjct: 361 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 420

Query: 421 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 480
           A+FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQ C+IKADKDL PV+KQ+RSFLG
Sbjct: 421 AIFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLG 480

Query: 481 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 540
           RPWKEYSRT+IM+SYI ++I P+GWLPW GDWGLKTCFY+E NN GPGS+KSKRVKWRGI
Sbjct: 481 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGI 540

Query: 541 KNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMV-SGGAA 578
           K ITPQHA  +TAGRF++GDRWIKPTG+PY+SGM  +GGAA
Sbjct: 541 KTITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 579

[Close]

BLAST of MC06g2206 vs. NCBI nr
Match: XP_038880188.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 458/588 (77.89%), Postives = 514/588 (87.41%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           M Y +DD+ N+KRYAIIGVSSMLLVAMV AVTVGVNLNQ ETSDPA GSKSHEI+ S KA
Sbjct: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK++C+ SLK+AG+NSSDPKEL+QAGF AA+KL+Q AA+KSVTLKEIEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           RTS+ALAGCKELM+FA+DELKYS  +LG+FDI+KLDEMLID+KIWLSATITYQETCLD F
Sbjct: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRR-LLELPVVVGHDD 240
           ENTTGNAAEKMKKALKTSM LSSNGLAMV+++SSMLT+LQIPGISRR LLE+PV+ GHDD
Sbjct: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVL-GHDD 240

Query: 241 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQG 300
           YP+WA+PG RRLL A+   +KPN VVAKDGSG++ TIQ+ALDKVPK K    YVI+IKQG
Sbjct: 241 YPDWANPGMRRLL-AAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQG 300

Query: 301 VYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIE 360
           VYKEY+ + K++T LM IGDGP KTIITG+KNF+DG PTFKTATV +TAE F+ARDIG E
Sbjct: 301 VYKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFE 360

Query: 361 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 420
           NTAGP KHQAVALRVQADK +FYNC MH YQDTLYVHTMRQFYRDC +SGTIDFVFGDAA
Sbjct: 361 NTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 420

Query: 421 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 480
           AVFQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQ  + KAD DL PV+KQ+RSFLG
Sbjct: 421 AVFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLG 480

Query: 481 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 540
           RPWKEYSRT+IM+SYI ++I P+GWLPW GDWGLKTCFY+E NN GPGSDKSKRVKWRGI
Sbjct: 481 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGI 540

Query: 541 KNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMVS-------GGAAH 579
           K ITPQHA  +TAGRFL GD WIK TG+PY+SGM         GGA H
Sbjct: 541 KTITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAARGGATH 586

[Close]

BLAST of MC06g2206 vs. NCBI nr
Match: XP_004135775.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044.1 hypothetical protein Csa_007353 [Cucumis sativus])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 449/585 (76.75%), Postives = 515/585 (88.03%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           MGY +DDS N+KRYAIIGVSSMLLVAMV AVTVGVNLNQ ETSDPATG+KSHEI++S KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK++C+ SLK+ G+NSSDPKEL+QAGF AA+KL+Q AA+KSV L ++EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           R S+ALAGCKELM+FAIDELKYS  +LG+FDI+KLDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRR-LLELPVVVGHDD 240
            NTTGNAAEKMKKALKTSM LSSNGLAMV+++SSML++LQIPGISRR LLE+PV+ GHDD
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVL-GHDD 240

Query: 241 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQG 300
           YP+WA+PG RRLL A+ + +KPN VVAKDGSG++ TIQ+A+D+VPK K    YVI+IK G
Sbjct: 241 YPDWANPGMRRLL-AAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAG 300

Query: 301 VYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIE 360
           VY+EY+ V KT+T LM IGDGP KTIITGNKNF+DG PTFKTATV +TAE F+ARDIG E
Sbjct: 301 VYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFE 360

Query: 361 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 420
           NTAGP+KHQAVALRVQADK +FYNC MH YQDTLYVHTMRQFYRDC VSGTIDF+FGDAA
Sbjct: 361 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 420

Query: 421 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 480
           A+FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQ C+ K   DL PV+KQ+RSFLG
Sbjct: 421 AIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLG 480

Query: 481 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 540
           RPWKEYSRT+IM+SYI ++I P+GWLPW GDWGL+TCFY+E NN GPGSDKSKRVKWRGI
Sbjct: 481 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGI 540

Query: 541 KNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMV----SGGAAH 579
           KNITPQHA  +T GRFL GDRWIKPTG+PY+SG+     +G AAH
Sbjct: 541 KNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583

[Close]

BLAST of MC06g2206 vs. NCBI nr
Match: XP_022969472.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 430/573 (75.04%), Postives = 495/573 (86.39%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           M Y++  + NRKRYAIIGVSSMLLVAMV AVTVGVNLNQ  TSDPATG K+HEI++S KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK +C++SLK +G   SDPKEL+Q GF AA+KL+Q AA KS+TL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           R SQALAGCKELM+F++DELK+S ++LG FDI+KLDEMLID+KIWLSA ITYQETCLD F
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRLLELPVVVGHDDY 240
            N+TG+A+EKMKKALK SM LSSNGLAMVTE+SSMLT+LQIPGI+RRLL +PV+ GH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVL-GHEDY 240

Query: 241 PEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQGV 300
           PEWA PG +RLL  SPA  KPN VVAKDGSG+Y TIQ+A++KVPK K    +VI+IKQGV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIEN 360
           YKEY+ + K +T LM IGDGP KTII+GNKNFVDG  TFKTATV ++AE F+ARDIG EN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADKV+F+NC MH YQDTLYVHTMRQFYRDC +SGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLGR 480
           VFQ C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQ    K D DL PVKKQ+RS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGIK 540
           PWKEYSRT+IM+SYID++I+P+GWLPW GDWGL+TCFY+E NN GPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 NITPQHAASYTAGRFLLGDRWIKPTGIPYISGM 572
           +IT QHA  +T GRF+LGDRWIK TGIPY+SGM
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGM 570

[Close]

BLAST of MC06g2206 vs. NCBI nr
Match: XP_022931680.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata])

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 430/573 (75.04%), Postives = 495/573 (86.39%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           M Y++  + NRKRYAIIGVSSMLLVAMV AVTVGVNLNQ  TSDPATG K+HEI++S KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK +C++SLK +G   SDPKEL+Q GF AA+KL+Q AA KSVTL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           R SQALA CKELM++++DELK+S ++LG FDI+KLDEMLID+KIWLSA ITYQETCLD F
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRLLELPVVVGHDDY 240
            NTTG+A+EKMKKALKTSM LSSNGLAMVTE+SSMLT+LQIPGI+RRLL +PV+ GH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVL-GHEDF 240

Query: 241 PEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQGV 300
           PEW  PG +RLL  SPA  KPN VVAKDGSG+Y TIQ+A++KVPK K    +VI+IKQGV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIEN 360
           YKEY+ + K +T LM IGDGP KTIITGNKNFVDG  TFKTATV ++AE F+ARDIG EN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADKV+F+NC MH YQDTLYVHTMRQFYRDC +SGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLGR 480
           VFQ C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQ    K D DL PVKKQ+RS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGIK 540
           PWKEYSRT+IM+SYID++IDP+GWLPW GDWGL+TCFY+E NN GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 NITPQHAASYTAGRFLLGDRWIKPTGIPYISGM 572
           +ITPQHA  +T GRF+LGDRWIK TG+PY+SGM
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 570

[Close]

BLAST of MC06g2206 vs. ExPASy TrEMBL
Match: A0A5D3CGC3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180 PE=3 SV=1)

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 458/581 (78.83%), Postives = 522/581 (89.85%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           MGY +DDS N+KRYAIIGVSSMLLVAMV AVTVGVNLNQ ETSDPATG+KSHEI++S KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK++C+ SLK+ G+NSSDP+EL+QAGF AA+KL+Q AA+KSV LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           RTS+ALAGCKELM+FAIDELKYS  +LG+FDITKLDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRR-LLELPVVVGHDD 240
           +NTTGNAAEKMKKALKTSM LSSNGLAMV++ SSMLT+LQIPGISRR LLE+PV+ GHDD
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVL-GHDD 240

Query: 241 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQG 300
           YP+WA+PG RRLL A+ + +KPN VVAKDGSG++ TIQ ALD+VPK K    YVI+IK G
Sbjct: 241 YPDWANPGMRRLL-AAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAG 300

Query: 301 VYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIE 360
           VY+EY+ + KT+T LM IGDGP KTIITGNKNFVDG PTFKTATV +TAE F+ARDIG E
Sbjct: 301 VYQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFE 360

Query: 361 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 420
           NTAGP+KHQAVALRVQADK +FYNC MH YQDTLYVHTMRQFYRDC VSGTIDF+FGDAA
Sbjct: 361 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 420

Query: 421 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 480
           A+FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQ C+IKADKDL PV+KQ+RSFLG
Sbjct: 421 AIFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLG 480

Query: 481 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 540
           RPWKEYSRT+IM+SYI ++I P+GWLPW GDWGLKTCFY+E NN GPGS+KSKRVKWRGI
Sbjct: 481 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGI 540

Query: 541 KNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMV-SGGAA 578
           K ITPQHA  +TAGRF++GDRWIKPTG+PY+SGM  +GGAA
Sbjct: 541 KTITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 579

[Close]

BLAST of MC06g2206 vs. ExPASy TrEMBL
Match: A0A1S3BPI8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1)

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 458/581 (78.83%), Postives = 522/581 (89.85%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           MGY +DDS N+KRYAIIGVSSMLLVAMV AVTVGVNLNQ ETSDPATG+KSHEI++S KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK++C+ SLK+ G+NSSDP+EL+QAGF AA+KL+Q AA+KSV LK++EKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           RTS+ALAGCKELM+FAIDELKYS  +LG+FDITKLDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRR-LLELPVVVGHDD 240
           +NTTGNAAEKMKKALKTSM LSSNGLAMV++ SSMLT+LQIPGISRR LLE+PV+ GHDD
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVL-GHDD 240

Query: 241 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQG 300
           YP+WA+PG RRLL A+ + +KPN VVAKDGSG++ TIQ ALD+VPK K    YVI+IK G
Sbjct: 241 YPDWANPGMRRLL-AAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAG 300

Query: 301 VYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIE 360
           VY+EY+ + KT+T LM IGDGP KTIITGNKNFVDG PTFKTATV +TAE F+ARDIG E
Sbjct: 301 VYQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFE 360

Query: 361 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 420
           NTAGP+KHQAVALRVQADK +FYNC MH YQDTLYVHTMRQFYRDC VSGTIDF+FGDAA
Sbjct: 361 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 420

Query: 421 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 480
           A+FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQ C+IKADKDL PV+KQ+RSFLG
Sbjct: 421 AIFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLG 480

Query: 481 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 540
           RPWKEYSRT+IM+SYI ++I P+GWLPW GDWGLKTCFY+E NN GPGS+KSKRVKWRGI
Sbjct: 481 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGI 540

Query: 541 KNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMV-SGGAA 578
           K ITPQHA  +TAGRF++GDRWIKPTG+PY+SGM  +GGAA
Sbjct: 541 KTITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 579

[Close]

BLAST of MC06g2206 vs. ExPASy TrEMBL
Match: A0A0A0LYF5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1)

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 449/585 (76.75%), Postives = 515/585 (88.03%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           MGY +DDS N+KRYAIIGVSSMLLVAMV AVTVGVNLNQ ETSDPATG+KSHEI++S KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK++C+ SLK+ G+NSSDPKEL+QAGF AA+KL+Q AA+KSV L ++EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           R S+ALAGCKELM+FAIDELKYS  +LG+FDI+KLDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRR-LLELPVVVGHDD 240
            NTTGNAAEKMKKALKTSM LSSNGLAMV+++SSML++LQIPGISRR LLE+PV+ GHDD
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVL-GHDD 240

Query: 241 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQG 300
           YP+WA+PG RRLL A+ + +KPN VVAKDGSG++ TIQ+A+D+VPK K    YVI+IK G
Sbjct: 241 YPDWANPGMRRLL-AAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAG 300

Query: 301 VYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIE 360
           VY+EY+ V KT+T LM IGDGP KTIITGNKNF+DG PTFKTATV +TAE F+ARDIG E
Sbjct: 301 VYQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFE 360

Query: 361 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 420
           NTAGP+KHQAVALRVQADK +FYNC MH YQDTLYVHTMRQFYRDC VSGTIDF+FGDAA
Sbjct: 361 NTAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAA 420

Query: 421 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 480
           A+FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQ C+ K   DL PV+KQ+RSFLG
Sbjct: 421 AIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLG 480

Query: 481 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 540
           RPWKEYSRT+IM+SYI ++I P+GWLPW GDWGL+TCFY+E NN GPGSDKSKRVKWRGI
Sbjct: 481 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGI 540

Query: 541 KNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMV----SGGAAH 579
           KNITPQHA  +T GRFL GDRWIKPTG+PY+SG+     +G AAH
Sbjct: 541 KNITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGAGAAAH 583

[Close]

BLAST of MC06g2206 vs. ExPASy TrEMBL
Match: A0A6J1HWF1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1)

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 430/573 (75.04%), Postives = 495/573 (86.39%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           M Y++  + NRKRYAIIGVSSMLLVAMV AVTVGVNLNQ  TSDPATG K+HEI++S KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK +C++SLK +G   SDPKEL+Q GF AA+KL+Q AA KS+TL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           R SQALAGCKELM+F++DELK+S ++LG FDI+KLDEMLID+KIWLSA ITYQETCLD F
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRLLELPVVVGHDDY 240
            N+TG+A+EKMKKALK SM LSSNGLAMVTE+SSMLT+LQIPGI+RRLL +PV+ GH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVL-GHEDY 240

Query: 241 PEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQGV 300
           PEWA PG +RLL  SPA  KPN VVAKDGSG+Y TIQ+A++KVPK K    +VI+IKQGV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIEN 360
           YKEY+ + K +T LM IGDGP KTII+GNKNFVDG  TFKTATV ++AE F+ARDIG EN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADKV+F+NC MH YQDTLYVHTMRQFYRDC +SGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLGR 480
           VFQ C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQ    K D DL PVKKQ+RS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGIK 540
           PWKEYSRT+IM+SYID++I+P+GWLPW GDWGL+TCFY+E NN GPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 NITPQHAASYTAGRFLLGDRWIKPTGIPYISGM 572
           +IT QHA  +T GRF+LGDRWIK TGIPY+SGM
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGM 570

[Close]

BLAST of MC06g2206 vs. ExPASy TrEMBL
Match: A0A6J1F058 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1)

HSP 1 Score: 882 bits (2278), Expect = 0.0
Identity = 430/573 (75.04%), Postives = 495/573 (86.39%), Query Frame = 0


Query: 1   MGYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKA 60
           M Y++  + NRKRYAIIGVSSMLLVAMV AVTVGVNLNQ  TSDPATG K+HEI++S KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDP 120
           IK IC   DYK +C++SLK +G   SDPKEL+Q GF AA+KL+Q AA KSVTL++IEKDP
Sbjct: 61  IKAICRPTDYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAF 180
           R SQALA CKELM++++DELK+S ++LG FDI+KLDEMLID+KIWLSA ITYQETCLD F
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRLLELPVVVGHDDY 240
            NTTG+A+EKMKKALKTSM LSSNGLAMVTE+SSMLT+LQIPGI+RRLL +PV+ GH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVL-GHEDF 240

Query: 241 PEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPK-KRKGVYVIYIKQGV 300
           PEW  PG +RLL  SPA  KPN VVAKDGSG+Y TIQ+A++KVPK K    +VI+IKQGV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIEN 360
           YKEY+ + K +T LM IGDGP KTIITGNKNFVDG  TFKTATV ++AE F+ARDIG EN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAA 420
           TAGP KHQAVALRVQADKV+F+NC MH YQDTLYVHTMRQFYRDC +SGTIDFVFGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLGR 480
           VFQ C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQ    K D DL PVKKQ+RS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGIK 540
           PWKEYSRT+IM+SYID++IDP+GWLPW GDWGL+TCFY+E NN GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 NITPQHAASYTAGRFLLGDRWIKPTGIPYISGM 572
           +ITPQHA  +T GRF+LGDRWIK TG+PY+SGM
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGM 570

[Close]

BLAST of MC06g2206 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 641.3 bits (1653), Expect = 7.2e-184
Identity = 308/578 (53.29%), Postives = 423/578 (73.18%), Query Frame = 0


Query: 2   GYNEDDSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKAI 61
           GY+++D+  +KRY II +SS+LL++MV AVT+GV++N+++ +         EITTS KAI
Sbjct: 4   GYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAI 63

Query: 62  KTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKDPR 121
           K +CA  DYKE C ++L+    ++SDP EL++  FNA +K +   A KS T+ E++KDPR
Sbjct: 64  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 123

Query: 122 TSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFE 181
              AL  CKELM++AI EL  SF+ LG F+  K+DE L+ L+IWLSATI++++TCLD F+
Sbjct: 124 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGI-SRRLLELPVVVGHDDY 241
            T GNA E +KKALKT++ L+ NGLAMVTEMS+ L  +QIP + SRRLL         ++
Sbjct: 184 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS-------QEF 243

Query: 242 PEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIKQGVY 301
           P W    +RRLL+A  + +KP+ VVA+DGSG+Y TI +AL+ VPKK+   +V++IK+G+Y
Sbjct: 244 PSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIY 303

Query: 302 KEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIGIENT 361
           KEY+ VN++MT L+FIGDGP KT+I+G+K++ DGI T+KTATV I  + FIA++I  ENT
Sbjct: 304 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 363

Query: 362 AGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAAAV 421
           AG  KHQAVA+RV AD+ IFYNC    YQDTLY H+ RQFYRDC +SGTIDF+FGDAAAV
Sbjct: 364 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 423

Query: 422 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLGRP 481
           FQ CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q CTI  + D   VK+Q +++LGRP
Sbjct: 424 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 483

Query: 482 WKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGIKN 541
           WKEYSRT+IM+++I + + P+GW PW G++GL T FYSEV N GPG+  +KRV W GIK 
Sbjct: 484 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 543

Query: 542 ITPQHAASYTAGRFLLGDRWIKPTGIPYISGMVSGGAA 579
           ++ +    +T  +++ GD WI   G+PYI G+ SG  +
Sbjct: 544 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGS 568

[Close]

BLAST of MC06g2206 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 630.2 bits (1624), Expect = 1.7e-180
Identity = 309/582 (53.09%), Postives = 415/582 (71.31%), Query Frame = 0


Query: 1   MGYNEDDSGNRK-RYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTK 60
           M Y  DD   RK RY +I +SS+LL++MV AVTVGV+LN+ +           E+  S K
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA----EVNASVK 60

Query: 61  AIKTICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKSVTLKEIEKD 120
           A+K +CA  DY++ C ++L   G N++DP EL++  FN  +K +  AA KS T+ E++KD
Sbjct: 61  AVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKD 120

Query: 121 PRTSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDA 180
            RT  AL  CKELM++A+DEL  SF+ LG F+   LDE LI+L+IWLSA I+++ETCL+ 
Sbjct: 121 SRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEG 180

Query: 181 FENTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGI-SRRLLELPVVVGHD 240
           F+ T GNA E MKKALKT++ L+ NGLA+++EMS+ +  +QIPG+ SRRLL        +
Sbjct: 181 FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-------E 240

Query: 241 DYPEWASPGSRRLLDASPA--SIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIK 300
            +P W     R+LL A+ A   +KP+ VVA+DGSG+Y TI +AL  VPKKR   +V++IK
Sbjct: 241 GFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIK 300

Query: 301 QGVYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDGIPTFKTATVVITAEDFIARDIG 360
            G+YKEY+ VNKTM+ L+FIGDGP KTII+GNKN+ DGI T++TATV I    FIA++IG
Sbjct: 301 AGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIG 360

Query: 361 IENTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGD 420
            ENTAG  KHQAVA+RVQ+D+ IF+NC    YQDTLY H+ RQF+RDC +SGTIDF+FGD
Sbjct: 361 FENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGD 420

Query: 421 AAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSF 480
           AAAVFQ CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q CTI  + D   VK+  +++
Sbjct: 421 AAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAY 480

Query: 481 LGRPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWR 540
           LGRPWKEYSRT+IM+++I + + PQGW PW GD+GLKT FYSEV N GPGS  + RV W 
Sbjct: 481 LGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWA 540

Query: 541 GIKNITPQHAASYTAGRFLLGDRWIKPTGIPYISGMVSGGAA 579
           GIK ++ +    +T  +++ GD WI   G+PY +G+++G  A
Sbjct: 541 GIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPA 571

[Close]

BLAST of MC06g2206 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 536.6 bits (1381), Expect = 2.5e-152
Identity = 270/571 (47.29%), Postives = 376/571 (65.85%), Query Frame = 0


Query: 6   DDSGNRKRYAIIGVSSMLLVAMVAAV--TVGVNLNQTETSDPATGSKSHEITTSTKAIKT 65
           D    +K+  I GV + LLV MV AV  T   N + +E   P       +I T+T A++ 
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICATVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADK-SVTL-KEIEKDPR 125
           +CA  DYKE C+NSL  A  +S+ P +LI+ GFN  ++ ++ +  K SV L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 TSQALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFE 185
           T  AL  C++LMN A D+LK        F I ++++ + DL++WLS +I YQ+TC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRL--LELPVVVGHDD 245
            T    ++ M+K  KTS  L+SNGLAM+T +S++L +  + G++  L      ++   D 
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG 243

Query: 246 YPEWASPGSRRLLDASPASIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIKQGV 305
            P W  P +RRL+ A+   +K N VVA DGSG+Y TI +AL+ VPK  +  +VIYIKQGV
Sbjct: 244 IPSWVGPNTRRLM-ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGV 303

Query: 306 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNFVDG-IPTFKTATVVITAEDFIARDIGIE 365
           Y E + V K MT + FIGDGPTKT ITG+ N+  G + T+ TATV I  ++F A++IG E
Sbjct: 304 YNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFE 363

Query: 366 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 425
           NTAGPE HQAVALRV AD  +FYNC +  YQDTLYVH+ RQF+RDC VSGT+DF+FGD  
Sbjct: 364 NTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGI 423

Query: 426 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 485
            V Q C  +VRKP+ +Q C++TA GR ++R+ + L++Q C I  +    PVK   +++LG
Sbjct: 424 VVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLG 483

Query: 486 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 545
           RPWKE+SRT+IM + ID+VIDP GWLPWNGD+ L T +Y+E  NNGPGS++++RVKW GI
Sbjct: 484 RPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGI 543

Query: 546 KNITPQHAASYTAGRFLLGDRWIKPTGIPYI 570
           K ++P+ A  +T  RFL G+ WI P  +PY+
Sbjct: 544 KKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

[Close]

BLAST of MC06g2206 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 505.8 bits (1301), Expect = 4.8e-143
Identity = 259/570 (45.44%), Postives = 368/570 (64.56%), Query Frame = 0


Query: 7   DSGNRKRYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKAIKTICA 66
           D   +K++ + G  S  LV MV +V V        TS  +     + I  +TKA++ +CA
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  TVDYKEDCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADK-SVTLK-EIEKDPRTSQ 126
             D+K+ C+NSL  A  +S DP +LI+ GF   +K + ++ +K S  +K + +K+P    
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFENTT 186
           A   C++LM  AID+LK        F + +++  + DL++WLS +I +Q+TC+D+F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMHLSSNGLAMVTEMSSMLTDLQIPGISRRLLEL--PVVVGHDDYPE 246
            N  + M K  KTS  LSSN LAMVT +S+++ +  + G++  L +    ++   D  P 
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPT 243

Query: 247 WASPGSRRLLDA---SPASIKPNAVVAKDGSGKYSTIQDALDKVPKKRKGVYVIYIKQGV 306
           W  P +RRL+ A    P  +K NAVVA+DG+G++ TI DAL+ VPK  K  ++I+IK+G+
Sbjct: 244 WVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGI 303

Query: 307 YKEYITVNKTMTKLMFIGDGPTKTIITGNKNF-VDGIPTFKTATVVITAEDFIARDIGIE 366
           YKE +TV K M  + FIGDGP KT+ITG+ NF +  + TF TAT+ I  + F A++IGIE
Sbjct: 304 YKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIE 363

Query: 367 NTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVSGTIDFVFGDAA 426
           NTAGPE  QAVALRV AD  +F++C +  +QDTLYVH+ RQFYRDC VSGT+DF+FGDA 
Sbjct: 364 NTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAK 423

Query: 427 AVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLEPVKKQYRSFLG 486
            + Q C  +VRKP   Q C+VTA GR   R+ + L++  C I  D    P+K   +++LG
Sbjct: 424 CILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLG 483

Query: 487 RPWKEYSRTLIMDSYIDNVIDPQGWLPWNGDWGLKTCFYSEVNNNGPGSDKSKRVKWRGI 546
           RPWKE+SRT+IM + ID+VIDP GWLPW+GD+ LKT +Y+E  N GPGS++++RVKW GI
Sbjct: 484 RPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGI 543

Query: 547 KNITPQHAASYTAGRFLLGDRWIKPTGIPY 569
           K +TPQ A  YT  RFL GD WI  T +PY
Sbjct: 544 KKLTPQDALLYTGDRFLRGDTWIPQTQVPY 564

[Close]

BLAST of MC06g2206 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 448.4 bits (1152), Expect = 9.0e-126
Identity = 269/701 (38.37%), Postives = 372/701 (53.07%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVAAVTVGVNLNQTETSDPATGSKSHEITTSTKAIKTICATVDYKE 72
           +Y ++GV++ L++AMV  V      + +   +         IT +T +I  ICA+ DYK+
Sbjct: 3   KYVLLGVTA-LIMAMVICVEANDGNSPSRKMEEVHRESKLMITKTTVSI--ICASTDYKQ 62

Query: 73  DCMNSLKSAGHNSSDPKELIQAGFNAAVKLLQQAADKS-VTLK-EIEKDPRTSQALAGCK 132
           DC  SL +    S DP+ LI++ F+ A+  ++   D+  + LK   + D  T +AL  C+
Sbjct: 63  DCTTSLATV--RSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 133 ELMNFAIDELKYSFKRLGDFDITKLDEMLIDLKIWLSATITYQETCLDAFENTTGNAAEK 192
           ELM+ AID+L+ +  +   F  T+L + + DL +WLS +ITYQ+TC+D FE     AA  
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 193 MKKALKTSMHLSSNGLAMVTEMSSMLTDLQIP----------------GISR-------- 252
           M++ ++   HL+SNGLA+   +  +L   +IP                G SR        
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 253 --------------------------------------RLLE------------------ 312
                                                 R L+                  
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 313 -------------LPV-------------------------------------------- 372
                        LP                                             
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 373 ---VVGHDDYPEWASPGSRRLLDASPAS--IKPNAVVAKDGSGKYSTIQDALDKVPKKRK 432
              +    ++P W +P SRRLL   P +  IK N VVAKDGSGK  TI  AL  VP K  
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVPMKNT 422

Query: 433 GVYVIYIKQGVYKEYITVNKTMTKLMFIGDGPTKTIITGNKNFV-DGIPTFKTATVVITA 492
             +VI+IK+GVYKE + V K M  +MF+GDGPTKT+ITG+  F+ D + T++TA+V +  
Sbjct: 423 KKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVNG 482

Query: 493 EDFIARDIGIENTAGPEKHQAVALRVQADKVIFYNCAMHAYQDTLYVHTMRQFYRDCVVS 552
           + F+A+DIG ENTAG  +HQAVALRV AD  +F+NC M+ YQDTLYVHT RQFYR+C VS
Sbjct: 483 DYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRVS 542

Query: 553 GTIDFVFGDAAAVFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQKCTIKADKDLE 569
           GTIDFVFGDA AVFQ C F++R+P+ +QQCIVTA GRK+RR+ + ++I    I  D    
Sbjct: 543 GTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASYL 602

The following BLAST results are available for this feature:

BLAST of MC06g2206 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E8Z8	1.0e-182	53.29	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q8GX86	2.4e-179	53.09	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	3.5e-151	47.29	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	6.7e-142	45.44	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
O23447	1.3e-124	38.37	Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of MC06g2206 vs. NCBI nr


Match Name	Identity	Description
XP_008450602.1	78.83	PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >K...	[more]
XP_038880188.1	77.89	probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida]	[more]
XP_004135775.1	76.75	putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044....	[more]
XP_022969472.1	75.04	putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima]	[more]
XP_022931680.1	75.04	putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata]	[more]

BLAST of MC06g2206 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A5D3CGC3	78.83	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180...	[more]
A0A1S3BPI8	78.83	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1	[more]
A0A0A0LYF5	76.75	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1	[more]
A0A6J1HWF1	75.04	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1	[more]
A0A6J1F058	75.04	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1	[more]

BLAST of MC06g2206 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27870.1	7.2e-184	53.29	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	1.7e-180	53.09	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	2.5e-152	47.29	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	4.8e-143	45.44	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	9.0e-126	38.37	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 56..208 e-value: 1.7E-33 score: 127.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 60..208 e-value: 9.8E-24 score: 84.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 54..211 e-value: 3.4E-21 score: 73.3
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 262..558 e-value: 8.5E-138 score: 458.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 55..213 e-value: 6.1E-31 score: 109.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 59..213
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 256..572 e-value: 1.4E-119 score: 401.2
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 15..574
None	No IPR available	PANTHER	PTHR31707:SF337	PECTINESTERASE	coord: 15..574
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 63..214 e-value: 1.08223E-40 score: 142.962
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 406..415
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 259..571

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g2206.1	MC06g2206.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity