MC06g2073 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g2073
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Protein kinase domain-containing protein
Location	MC06: 28203482 .. 28206135 (+)
RNA-Seq Expression	MC06g2073
Synteny	MC06g2073

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCCACATGCTCAAACTAATTCTTCTGGTTGCTGCGGCCCCCATTTTTCTAATTTTCGTGATTTTGGTCTTCTTGTTCCGCCACAAGCTCGCAAAAACAGAGCAGAGAGATGTAGAGAAGGCGGCGGCGGCGAAGGCAGAGGAGGAGGAGGAGCTGAGTACTTTTGAGGGCGGCGAGGATTTGACGGTGGACGAGATTCTGGAAGCTCCGGGGGAGGTGATCGGAAAATCAAAGTATGGAACTCTGTACAAGGCGTCGCTGCAGAGAAGCCAGAGGACGCGGGTACTGAGATTCCTCCGACCCGCCGCCGCCACGGAAGAATCCGGCGACGACACCGTTGGGTTTCTGGGTTCGATTCGGCACCCGAATCTGGTTCCGATGTTGGGATTCTACGCCGGCCGCCGCGGCGAGAAGCTTCTGGTTCATCCCTTTTACGGCCGGGGGAATCTGGCAGACTTCATTACAGGTAATCCAATTTCTGTTTAATTCCCATTTCGGTTCATTCTTCCCATTTCCATTGCTCAATTTCCAAATTTCCCATTTTCAGTTTTATTCTATTTTCATCCACTTCTAAAACCTCTATTTTTATCTTAAAATTTTCAAAACTAAATCATTTTAGATTTTCTTTTTCTCTCTCTTTTTACAACAAATAGGACTAGGAGAGTTTGAATCAAACTAAACGGAAACATGTGTTAATGGGGCTCTCCTTTTTCTTTTTTTTTCTTTGTGTTTTTGGTGCATGTCGGCAACTATAATTTTTGTCTAGTTTCGGTATGCTGGCGTTGTGAATGGAGGAGATGACTCGGGAAGCGGGCGCGTTCTCCAACCCTCGTTCTTTTTTTTTTTTTTTTGTCTTAATCTTTCTCTCTAATGGGAGAGAGAAAAATTTTCCCTCTATTTAATTTTCTTTTCCTAAGTAAGCATGTCGTTTTTTAATTTTTTTTTTACAATGTAGTATTTTCATACTGTTTTTTATTTTATTTTGATTTGTTTCGTTACTTTCCATTTGGATTAACTAGTTTTGATATTTTCAAAATGTTTGATTTATAAATGGTCACTTTTTAAAAATATATAAATCAAAACAAACATATTTTTCTTTTAGAAAAATAGAAATACAATCTTGTGAAATTGTCTAATATTGACAACTAAGTGATAATAATGGATTTTTTTTTCTCTAAAATATAATTTTGGTCTTTATATTTTAGTTTGGATTCTTTGATCATGTAATTTTAAAATATTCATTTAGTCTATATATTTTTAAAAGGTTCACTTCTGTTCCTTTATTTTTAAGAGTGGATTATTTTAATTTCTTAATTTTATTGTTAATCCTATTTTTTATATTATAATTTTAACACGGTACTTAATATATTTTTTGAAATGAAACCATTACTTTATATTAAAGGTTGGTTTTGGTTAGGTATAAAACTTTAGTTAAAATTTTGTAGGAATACATTGAGGTAAAGGGACTAAAATGAATCAAAATGAATATTTTGAAAGTACACGAAGTAAAATGAACCAAAGTTTAGGAAAAATGACAAGGACGAATATAAACAAAGGCTGAAAATAGAGGAAACAAATATATTTTAACTTTTTTTTAAGTATAAAATAATTGTTTTATTGTTTAAAAAATAAGTTTATTGAGTACTTTATTATTCTTCTTTTTTTAATCCACATAAATTTTCAAGAAAAATAAAAGGAAATAGTCTTAAATTCTTCTCTATTTCTTAAATTTAGAAAAAGAAGTAGGAATTTAATTAATTCTTTTTTTTTTCTTTTTTTACTTGTGATTGAACAGTGGGAGAAGCAGAAGCAGAATCAGAGAGATGGGAAATCATTTACAAAATCTCCATGGGAATCGCCAAAGCCTTGGATCATCTCCACAGTGAATTGCAGAAACCGTTGGCGCACGGCAATCTCAAGTCCAAGAACATTCTTCTCGATCGGAATTTCGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAATCCCGCCGCAAATCAAGAACTGCTCCGAGTTTCCGCCGATTCCGGCTACAAGGCGCCGGAGCTGATCAAGATGACGGACGCCGATGAGCGGACCGACGTCTACAGCTACGGCAAAATCCTTCTCGAGTTGTTGCTGTACGGCAAAGATCAGCCGTCGAATCGAAACTCACCTCCCGACGGAGACGGCGGCGATTGCGGTGTTTCTGATGACCGGATTCTCCGATGCTCTCGGATCGTCGAGGCTTGTTGTTCTTCGTCGCCGGATCTTCGGCCGAATTTCAAGGAAGTGATTGCGAAGATTGAGGAAATCGGAAGGATTAAGATCGATTGACTGCGGCAAAGAGGGCTAGGGTTAGAACTCTATGAGAGGTGATTTTCGTTTTTCCAAAATGAATTTTGAGAAATTGATCTTTTTTTTTTTAATTATTCTTTTTCTTGATTCTACAGAATGAATGAATATGGCTGACTTTTTTCCCCTTTCTTTAGTCTAATTTTTGTTTTTCTTATTGATTGCTGTATATATCAACAATGGGTATTTAGGGTCCTGATTTGATTTGATTCTTTTAATTAAAGAAATTAATTTATTGAAAGTATTTTTCTTTTAGTTCACAACATGTTGTCCTAAAAGGATATATGCCCTACCAGCTAATTTCTTGTTTTAGG

mRNA sequence

ACCCACATGCTCAAACTAATTCTTCTGGTTGCTGCGGCCCCCATTTTTCTAATTTTCGTGATTTTGGTCTTCTTGTTCCGCCACAAGCTCGCAAAAACAGAGCAGAGAGATGTAGAGAAGGCGGCGGCGGCGAAGGCAGAGGAGGAGGAGGAGCTGAGTACTTTTGAGGGCGGCGAGGATTTGACGGTGGACGAGATTCTGGAAGCTCCGGGGGAGGTGATCGGAAAATCAAAGTATGGAACTCTGTACAAGGCGTCGCTGCAGAGAAGCCAGAGGACGCGGGTACTGAGATTCCTCCGACCCGCCGCCGCCACGGAAGAATCCGGCGACGACACCGTTGGGTTTCTGGGTTCGATTCGGCACCCGAATCTGGTTCCGATGTTGGGATTCTACGCCGGCCGCCGCGGCGAGAAGCTTCTGGTTCATCCCTTTTACGGCCGGGGGAATCTGGCAGACTTCATTACAGTGGGAGAAGCAGAAGCAGAATCAGAGAGATGGGAAATCATTTACAAAATCTCCATGGGAATCGCCAAAGCCTTGGATCATCTCCACAGTGAATTGCAGAAACCGTTGGCGCACGGCAATCTCAAGTCCAAGAACATTCTTCTCGATCGGAATTTCGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAATCCCGCCGCAAATCAAGAACTGCTCCGAGTTTCCGCCGATTCCGGCTACAAGGCGCCGGAGCTGATCAAGATGACGGACGCCGATGAGCGGACCGACGTCTACAGCTACGGCAAAATCCTTCTCGAGTTGTTGCTGTACGGCAAAGATCAGCCGTCGAATCGAAACTCACCTCCCGACGGAGACGGCGGCGATTGCGGTGTTTCTGATGACCGGATTCTCCGATGCTCTCGGATCGTCGAGGCTTGTTGTTCTTCGTCGCCGGATCTTCGGCCGAATTTCAAGGAAGTGATTGCGAAGATTGAGGAAATCGGAAGGATTAAGATCGATTGACTGCGGCAAAGAGGGCTAGGGTTAGAACTCTATGAGAGAATGAATGAATATGGCTGACTTTTTTCCCCTTTCTTTAGTCTAATTTTTGTTTTTCTTATTGATTGCTGTATATATCAACAATGGGTATTTAGGGTCCTGATTTGATTTGATTCTTTTAATTAAAGAAATTAATTTATTGAAAGTATTTTTCTTTTAGTTCACAACATGTTGTCCTAAAAGGATATATGCCCTACCAGCTAATTTCTTGTTTTAGG

Coding sequence (CDS)

ACCCACATGCTCAAACTAATTCTTCTGGTTGCTGCGGCCCCCATTTTTCTAATTTTCGTGATTTTGGTCTTCTTGTTCCGCCACAAGCTCGCAAAAACAGAGCAGAGAGATGTAGAGAAGGCGGCGGCGGCGAAGGCAGAGGAGGAGGAGGAGCTGAGTACTTTTGAGGGCGGCGAGGATTTGACGGTGGACGAGATTCTGGAAGCTCCGGGGGAGGTGATCGGAAAATCAAAGTATGGAACTCTGTACAAGGCGTCGCTGCAGAGAAGCCAGAGGACGCGGGTACTGAGATTCCTCCGACCCGCCGCCGCCACGGAAGAATCCGGCGACGACACCGTTGGGTTTCTGGGTTCGATTCGGCACCCGAATCTGGTTCCGATGTTGGGATTCTACGCCGGCCGCCGCGGCGAGAAGCTTCTGGTTCATCCCTTTTACGGCCGGGGGAATCTGGCAGACTTCATTACAGTGGGAGAAGCAGAAGCAGAATCAGAGAGATGGGAAATCATTTACAAAATCTCCATGGGAATCGCCAAAGCCTTGGATCATCTCCACAGTGAATTGCAGAAACCGTTGGCGCACGGCAATCTCAAGTCCAAGAACATTCTTCTCGATCGGAATTTCGAGCCGCACGTCTCCGATTTCGGCCTCCATCTCCTCCTCAATCCCGCCGCAAATCAAGAACTGCTCCGAGTTTCCGCCGATTCCGGCTACAAGGCGCCGGAGCTGATCAAGATGACGGACGCCGATGAGCGGACCGACGTCTACAGCTACGGCAAAATCCTTCTCGAGTTGTTGCTGTACGGCAAAGATCAGCCGTCGAATCGAAACTCACCTCCCGACGGAGACGGCGGCGATTGCGGTGTTTCTGATGACCGGATTCTCCGATGCTCTCGGATCGTCGAGGCTTGTTGTTCTTCGTCGCCGGATCTTCGGCCGAATTTCAAGGAAGTGATTGCGAAGATTGAGGAAATCGGAAGGATTAAGATCGATTGA

Protein sequence

THMLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRILRCSRIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID

Homology

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BLAST of MC06g2073 vs. ExPASy Swiss-Prot
Match: P33543 (Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.5e-45
Identity = 106/314 (33.76%), Postives = 168/314 (53.50%), Query Frame = 0


Query: 34  EQRDVEKAAAAKAEEEEELSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRT 93
           E+ D E     K   E +L  F+GGE+LT+D++L A G+V+ K+ YGT+YKA L      
Sbjct: 339 EEGDEEDEIGEKEGGEGKLVVFQGGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNI 398

Query: 94  RVLRFLRPAAATEESGD-DTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLAD 153
             LR LR     + S     +  LG IRH NLVP+  FY G+RGEKLL++ +    +L D
Sbjct: 399 -ALRLLREGTCKDRSSCLPVIRQLGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHD 458

Query: 154 FITVGEAEAESERWEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVS 213
            +   +    +  W   +KI++GIA+ L +LH+  + P+ HGN++SKN+L+D  F   ++
Sbjct: 459 LLHESKPRKPALNWARRHKIALGIARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLT 518

Query: 214 DFGLHLLLNPAANQELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQP 273
           +FGL  ++  A   E++  +   GYKAPEL KM   + R+DVY++G +LLE+L+  K   
Sbjct: 519 EFGLDKIMVQAVADEIVSQAKSDGYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGK 578

Query: 274 SNRNSPPDGD-----------------------GGDCGVSDDRILRCSRIVEACCSSSPD 324
           S RN     D                        G     ++ ++   ++   CC+    
Sbjct: 579 SGRNGNEFVDLPSLVKAAVLEETTMEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTT 638

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BLAST of MC06g2073 vs. ExPASy Swiss-Prot
Match: C0LGP9 (Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis thaliana OX=3702 GN=IMK3 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.5e-41
Identity = 115/355 (32.39%), Postives = 179/355 (50.42%), Query Frame = 0


Query: 5   KLILLVAAAPIFLIFVILV----FLFRHKLAKTEQRDVEK---AAAAKAEEEEE------ 64
           K I+L+A+  + ++ +ILV     L R K  +T+ +  E    A AAK E+  E      
Sbjct: 408 KDIILIASGALLIVMLILVCVLCCLLRKKANETKAKGGEAGPGAVAAKTEKGGEAEAGGE 467

Query: 65  ----LSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEE 124
               L  F+G    T D++L A  E++GKS YGT+YKA+L+   +  V R       +++
Sbjct: 468 TGGKLVHFDGPMAFTADDLLCATAEIMGKSTYGTVYKATLEDGSQVAVKRLREKITKSQK 527

Query: 125 SGDDTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWE 184
             ++ +  LG IRHPNL+ +  +Y G +GEKL+V  +  RG+LA F+     +     W 
Sbjct: 528 EFENEINVLGRIRHPNLLALRAYYLGPKGEKLVVFDYMSRGSLATFLHARGPDVHI-NWP 587

Query: 185 IIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQE 244
               +  G+A+ L +LH+     + HGNL S N+LLD N    +SD+GL  L+  AA   
Sbjct: 588 TRMSLIKGMARGLFYLHTHAN--IIHGNLTSSNVLLDENITAKISDYGLSRLMTAAAGSS 647

Query: 245 LLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELL--------LYGKDQPSNRNSPP 304
           ++  +   GY+APEL K+  A+ +TDVYS G I+LELL        L G D P    +  
Sbjct: 648 VIATAGALGYRAPELSKLKKANTKTDVYSLGVIILELLTGKSPSEALNGVDLPQWVATAV 707

Query: 305 DGDG----------GDCGVSDDRILRCSRIVEACCSSSPDLRPNFKEVIAKIEEI 325
             +            D     D IL   ++   C  ++P  RP  ++V+ ++ EI
Sbjct: 708 KEEWTNEVFDLELLNDVNTMGDEILNTLKLALHCVDATPSTRPEAQQVMTQLGEI 759

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BLAST of MC06g2073 vs. ExPASy Swiss-Prot
Match: Q9SCT4 (Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabidopsis thaliana OX=3702 GN=IMK2 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 7.9e-39
Identity = 106/312 (33.97%), Postives = 154/312 (49.36%), Query Frame = 0


Query: 32  KTEQRDVEKAAAAKAEEEEELSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQ 91
           KT    V   A+A  E   +L  F+G    T D++L A  E++GKS YGT YKA+L+   
Sbjct: 501 KTVSAGVAGTASAGGEMGGKLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGN 560

Query: 92  RTRVLRFLRPAAATEESGDDTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLA 151
              V R         +  +  V  LG IRH NL+ +  +Y G +GEKLLV  +  +G+L+
Sbjct: 561 EVAVKRLREKTTKGVKEFEGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLS 620

Query: 152 DFITVGEAEAESERWEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHV 211
            F+     E     WE   KI+ GI++ L HLHS   + + H NL + NILLD     H+
Sbjct: 621 AFLHARGPETLIP-WETRMKIAKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHI 680

Query: 212 SDFGLHLLLNPAANQELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKD- 271
           +D+GL  L+  AA   ++  +   GY+APE  K+ +A  +TDVYS G I+LELL  GK  
Sbjct: 681 ADYGLSRLMTAAAATNVIATAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLT-GKSP 740

Query: 272 -QPSNRNSPPDGDGG-----------------DCGVSDDRILRCSRIVEACCSSSPDLRP 325
            +P+N    P                      +     D +L   ++   C   SP  RP
Sbjct: 741 GEPTNGMDLPQWVASIVKEEWTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARP 800

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BLAST of MC06g2073 vs. ExPASy Swiss-Prot
Match: Q9SUQ3 (Probable inactive receptor kinase At4g23740 OS=Arabidopsis thaliana OX=3702 GN=At4g23740 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 9.4e-32
Identity = 101/328 (30.79%), Postives = 155/328 (47.26%), Query Frame = 0


Query: 28  HKLAKTEQRDVEKAAAAKAEEEEELSTFEG-GEDLTVDEILEAPGEVIGKSKYGTLYKAS 87
           +KL K      EK  +   +    LS FEG      ++++L A  EV+GK  +GT YKA 
Sbjct: 296 NKLQKKGGMSPEKFVSRMEDVNNRLSFFEGCNYSFDLEDLLRASAEVLGKGTFGTTYKAV 355

Query: 88  LQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYG 147
           L+ +    V R L+  AA +   +  +  +G I+H N+V +  +Y   + EKL+V+ ++ 
Sbjct: 356 LEDATSVAVKR-LKDVAAGKRDFEQQMEIIGGIKHENVVELKAYYYS-KDEKLMVYDYFS 415

Query: 148 RGNLADFITVGEAEAESE-RWEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDR 207
           RG++A  +     E      WE   KI++G AK +  +H E    L HGN+KS NI L+ 
Sbjct: 416 RGSVASLLHGNRGENRIPLDWETRMKIAIGAAKGIARIHKENNGKLVHGNIKSSNIFLNS 475

Query: 208 NFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELL 267
                VSD GL  +++P A      +S  +GY+APE+     + + +DVYS+G +LLELL
Sbjct: 476 ESNGCVSDLGLTAVMSPLAPP----ISRQAGYRAPEVTDTRKSSQLSDVYSFGVVLLELL 535

Query: 268 LYGKDQPSNRNSPPDGDGGD------------------CGVSDDRILRCSRIVE------ 326
             GK       SP     GD                    V D  +LR + I E      
Sbjct: 536 T-GK-------SPIHTTAGDEIIHLVRWVHSVVREEWTAEVFDIELLRYTNIEEEMVEML 595

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BLAST of MC06g2073 vs. ExPASy Swiss-Prot
Match: Q9ZRF9 (Probable LRR receptor-like serine/threonine-protein kinase RPK1 OS=Arabidopsis thaliana OX=3702 GN=RPK1 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.7e-29
Identity = 83/268 (30.97%), Postives = 141/268 (52.61%), Query Frame = 0


Query: 4   LKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTF-EGGEDLT 63
           +++  +V+A+ I  + ++LV LF +       R  ++ +  + +E +E+  F + G  LT
Sbjct: 197 IEIASIVSASVIVFVLLVLVILFIY------TRKWKRNSQVQVDEIKEIKVFVDIGIPLT 256

Query: 64  VDEILEAPG-----EVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLG 123
            + I+ A G       IG   +G+ YKA +  +    V R        ++     +  L 
Sbjct: 257 YEIIVRATGYFSNSNCIGHGGFGSTYKAEVSPTNVFAVKRLSVGRFQGDQQFHAEISALE 316

Query: 124 SIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIA 183
            +RHPNLV ++G++A    E  L++ +   GNL DFI   E    +  W++++KI++ +A
Sbjct: 317 MVRHPNLVMLIGYHASET-EMFLIYNYLSGGNLQDFIK--ERSKAAIEWKVLHKIALDVA 376

Query: 184 KALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGY 243
           +AL +LH +    + H ++K  NILLD N+  ++SDFGL  LL  + +     V+   GY
Sbjct: 377 RALSYLHEQCSPKVLHRDIKPSNILLDNNYNAYLSDFGLSKLLGTSQSHVTTGVAGTFGY 436

Query: 244 KAPELIKMTDADERTDVYSYGKILLELL 266
            APE        E+ DVYSYG +LLEL+
Sbjct: 437 VAPEYAMTCRVSEKADVYSYGIVLLELI 455

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BLAST of MC06g2073 vs. NCBI nr
Match: XP_022156696.1 (putative kinase-like protein TMKL1 [Momordica charantia])

HSP 1 Score: 638 bits (1645), Expect = 2.38e-230
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTFEGGEDLT 62
           MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTFEGGEDLT
Sbjct: 1   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTFEGGEDLT 60

Query: 63  VDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHP 122
           VDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHP
Sbjct: 61  VDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHP 120

Query: 123 NLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIAKALDH 182
           NLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIAKALDH
Sbjct: 121 NLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIAKALDH 180

Query: 183 LHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPEL 242
           LHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPEL
Sbjct: 181 LHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPEL 240

Query: 243 IKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRILRCSRIVEA 302
           IKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRILRCSRIVEA
Sbjct: 241 IKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRILRCSRIVEA 300

Query: 303 CCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           CCSSSPDLRPNFKEVIAKIEEIGRIKID
Sbjct: 301 CCSSSPDLRPNFKEVIAKIEEIGRIKID 328

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BLAST of MC06g2073 vs. NCBI nr
Match: XP_023532398.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419 bits (1077), Expect = 8.92e-144
Identity = 233/338 (68.93%), Postives = 265/338 (78.40%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQR-DVE--------KAAAAKAEEEEELS 62
           MLKLIL+VA+A IF++ ++ +F FRHKL+KT  R D+E        K     A   EEL+
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 63  TFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTV 122
           TFEGGEDLT+DEILEAPGEVIGK  YGTLYKASLQ +QRT +L FLRPAA  E S DD V
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAV-ESSVDDVV 120

Query: 123 GFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKIS 182
            FLGSIRH NLVPMLGFYAG RGE+LLVHPFYGRGNL++FITVGE+E  S+ WEIIYKIS
Sbjct: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESE--SKNWEIIYKIS 180

Query: 183 MGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSA 242
           +GIAKALDHLH  L K + HGNLK KNI LD NFEPHVSDFGLHLLLN AA +ELL+ S+
Sbjct: 181 LGIAKALDHLHHGLPKSVPHGNLKPKNIFLDPNFEPHVSDFGLHLLLNAAATRELLQASS 240

Query: 243 DSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRI 302
            SGY APEL++MTDADERTDVYSYGKILLELL   KD  +       GDGGDC VS +R 
Sbjct: 241 ASGYNAPELVRMTDADERTDVYSYGKILLELLS-AKDTMNLTAVACGGDGGDCSVSKERN 300

Query: 303 LRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           L+C  +I EACCSSSP  RP+FKEVI KIEEIGRI++D
Sbjct: 301 LQCLFQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

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BLAST of MC06g2073 vs. NCBI nr
Match: XP_023520536.1 (putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550283.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023550284.1 putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419 bits (1076), Expect = 5.46e-143
Identity = 233/342 (68.13%), Postives = 270/342 (78.95%), Query Frame = 0


Query: 1   THMLKLILLVAAAPIFLIFVILVFLF--RHKLAKTEQR-DVEKAAAAKAEEEEE------ 60
           +HMLK ILLVA+A IF++ +I +F F  RHKL+ TE R DVE+    +  ++E       
Sbjct: 46  SHMLKFILLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSP 105

Query: 61  --LSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESG 120
             L+TFEGGEDLT+DEILEAPGEVIGKS YGTLYKASLQ +Q TR+LRFLRP A   E  
Sbjct: 106 PSLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEY- 165

Query: 121 DDTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEII 180
            D V FLGSIRH NLVPMLGFYAG RGE+LLVHPFYG+GNLA+ I   E E+ES+ WE+I
Sbjct: 166 -DVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIR--ERESESKNWEVI 225

Query: 181 YKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELL 240
            KIS+GIA+ALDHLH+ LQKP+ HGNLKSKNILLD NFEPHVSDFGLHLLLN AA +E+L
Sbjct: 226 NKISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREML 285

Query: 241 RVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVS 300
           +VSA SGYKAPELI M+DADERTDV+SYGKILLELL   +           GDGGDCGVS
Sbjct: 286 QVSAASGYKAPELINMSDADERTDVFSYGKILLELLSAKE---------TGGDGGDCGVS 345

Query: 301 DDRILRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           ++R LRC  +I EACCSSSPDLRP+FKEVIAKI EIGRI++D
Sbjct: 346 EERTLRCLLQIGEACCSSSPDLRPDFKEVIAKITEIGRIEMD 374

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BLAST of MC06g2073 vs. NCBI nr
Match: KAG6605638.1 (putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035548.1 putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416 bits (1069), Expect = 1.47e-142
Identity = 231/338 (68.34%), Postives = 265/338 (78.40%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQR-DVE--------KAAAAKAEEEEELS 62
           MLKLIL+VA+A IF++ ++ +F FRHKL+KT  R D+E        K     A   EEL+
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFFRHKLSKTNHRHDIENPSPDNNQKKQDTAAATVEELT 60

Query: 63  TFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTV 122
           TFEGGEDLT+DEILEAPGEVIGK  YGTLYKASLQ +QRT +L FLRPAA  E S DD V
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAV-ESSVDDVV 120

Query: 123 GFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKIS 182
            FLGSIRH NLVPMLGFYAG RGE+LLVHPFYGRGNL++FITVGE+E  S+ WEIIYKIS
Sbjct: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESE--SKNWEIIYKIS 180

Query: 183 MGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSA 242
           +GIAKALDHLH  L KP+ HGNLK KNI LD +FEP+VSDFGLHLLLN AA +ELL+ S+
Sbjct: 181 LGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASS 240

Query: 243 DSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRI 302
            SGY APEL++MTDADERTDVYSYGKILLELL   KD  +       GDGGDC VS +R 
Sbjct: 241 ASGYNAPELVRMTDADERTDVYSYGKILLELLS-AKDTMNLTAVACGGDGGDCSVSKERN 300

Query: 303 LRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           L+C  +I EACC SSP  RP+FKEVI KIEEIGRI++D
Sbjct: 301 LQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

[Close]

BLAST of MC06g2073 vs. NCBI nr
Match: XP_022957896.1 (putative kinase-like protein TMKL1 [Cucurbita moschata])

HSP 1 Score: 416 bits (1068), Expect = 2.08e-142
Identity = 231/338 (68.34%), Postives = 265/338 (78.40%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQR-DVE--------KAAAAKAEEEEELS 62
           MLKLIL+VA+A IF++ ++ +F  RHKL+KT  R D+E        K     A   EEL+
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFLRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 63  TFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTV 122
           TFEGGEDLT+DEILEAPGEVIGK  YGTLYKASLQ +QRT +L FLRPAA  E S DD V
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAV-ESSVDDVV 120

Query: 123 GFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKIS 182
            FLGSIRH NLVPMLGFYAG RGE+LLVHPFYGRGNL++FITVGE+E  S+ WEIIYKIS
Sbjct: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESE--SKNWEIIYKIS 180

Query: 183 MGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSA 242
           +GIAKALDHLH  L KP+ HGNLK KNI LD +FEP+VSDFGLHLLLN AA +ELL+ S+
Sbjct: 181 LGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASS 240

Query: 243 DSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRI 302
            SGY APEL++MTDADERTDVYSYGKILLELL   KD  +       GDGGDC VS +R 
Sbjct: 241 ASGYNAPELVRMTDADERTDVYSYGKILLELLS-AKDTMNLTAVACGGDGGDCSVSKERN 300

Query: 303 LRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           L+C  +I EACCSSSP  RP+FKEVI KIEEIGRI++D
Sbjct: 301 LQCLFQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

[Close]

BLAST of MC06g2073 vs. ExPASy TrEMBL
Match: A0A6J1DVQ7 (putative kinase-like protein TMKL1 OS=Momordica charantia OX=3673 GN=LOC111023538 PE=4 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 1.15e-230
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTFEGGEDLT 62
           MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTFEGGEDLT
Sbjct: 1   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQRDVEKAAAAKAEEEEELSTFEGGEDLT 60

Query: 63  VDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHP 122
           VDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHP
Sbjct: 61  VDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGFLGSIRHP 120

Query: 123 NLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIAKALDH 182
           NLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIAKALDH
Sbjct: 121 NLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISMGIAKALDH 180

Query: 183 LHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPEL 242
           LHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPEL
Sbjct: 181 LHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSADSGYKAPEL 240

Query: 243 IKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRILRCSRIVEA 302
           IKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRILRCSRIVEA
Sbjct: 241 IKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRILRCSRIVEA 300

Query: 303 CCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           CCSSSPDLRPNFKEVIAKIEEIGRIKID
Sbjct: 301 CCSSSPDLRPNFKEVIAKIEEIGRIKID 328

[Close]

BLAST of MC06g2073 vs. ExPASy TrEMBL
Match: A0A6J1H0H8 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294 PE=4 SV=1)

HSP 1 Score: 416 bits (1068), Expect = 1.01e-142
Identity = 231/338 (68.34%), Postives = 265/338 (78.40%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQR-DVE--------KAAAAKAEEEEELS 62
           MLKLIL+VA+A IF++ ++ +F  RHKL+KT  R D+E        K     A   EEL+
Sbjct: 1   MLKLILIVASATIFVLLLVSIFFLRHKLSKTNHRNDIENPSPDNNQKKQDTAAATVEELT 60

Query: 63  TFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTV 122
           TFEGGEDLT+DEILEAPGEVIGK  YGTLYKASLQ +QRT +L FLRPAA  E S DD V
Sbjct: 61  TFEGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRTTLLMFLRPAAV-ESSVDDVV 120

Query: 123 GFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKIS 182
            FLGSIRH NLVPMLGFYAG RGE+LLVHPFYGRGNL++FITVGE+E  S+ WEIIYKIS
Sbjct: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESE--SKNWEIIYKIS 180

Query: 183 MGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSA 242
           +GIAKALDHLH  L KP+ HGNLK KNI LD +FEP+VSDFGLHLLLN AA +ELL+ S+
Sbjct: 181 LGIAKALDHLHHGLPKPVPHGNLKPKNIFLDPDFEPYVSDFGLHLLLNAAATRELLQASS 240

Query: 243 DSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRI 302
            SGY APEL++MTDADERTDVYSYGKILLELL   KD  +       GDGGDC VS +R 
Sbjct: 241 ASGYNAPELVRMTDADERTDVYSYGKILLELLS-AKDTMNLTAVACGGDGGDCSVSKERN 300

Query: 303 LRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           L+C  +I EACCSSSP  RP+FKEVI KIEEIGRI++D
Sbjct: 301 LQCLFQIGEACCSSSPIRRPDFKEVIEKIEEIGRIEMD 334

[Close]

BLAST of MC06g2073 vs. ExPASy TrEMBL
Match: A0A6J1I3Q5 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 PE=4 SV=1)

HSP 1 Score: 416 bits (1068), Expect = 1.16e-142
Identity = 232/341 (68.04%), Postives = 268/341 (78.59%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLF---RHKLAKTEQR-DVEKAAAAKAEEEEE------- 62
           MLK ILLVA+A IF++ +I +F F   RHKL+ TE R DVE+    + +++E        
Sbjct: 1   MLKFILLVASAAIFVLLLISIFFFFFFRHKLSLTEHRHDVERPCTDQNKKKEHTPPPSPP 60

Query: 63  -LSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGD 122
            L+TFEGGEDLT+DEILEAPGEVIGKS YGTLYKASLQ +Q TR+LRFLRP A   E   
Sbjct: 61  SLATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQSTRLLRFLRPGAGGGEY-- 120

Query: 123 DTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIY 182
           D V FLGSIRH NLVPMLGFYAG RGE+LLVHPFYG+GNLAD I VGE+E ++  WE+I 
Sbjct: 121 DVVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLADLIRVGESETKN--WEVIN 180

Query: 183 KISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLR 242
           KIS+GIA+ALDHLH+ LQKP+ HGNLKSKNILLD NFEPHVSDFGLHLLLN AA +E+L+
Sbjct: 181 KISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLQ 240

Query: 243 VSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSD 302
           VSA SGYKAPELI M DADERTD++SYGKILLELL   +           GDGGDCGVS+
Sbjct: 241 VSAASGYKAPELINMNDADERTDIFSYGKILLELLSAKE---------TGGDGGDCGVSE 300

Query: 303 DRILRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           +R LRC  +I EAC SSSPDLRPNFKEVIAKI EIGRI++D
Sbjct: 301 ERTLRCILQIGEACSSSSPDLRPNFKEVIAKIAEIGRIEMD 328

[Close]

BLAST of MC06g2073 vs. ExPASy TrEMBL
Match: A0A6J1K4E4 (putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 PE=4 SV=1)

HSP 1 Score: 411 bits (1057), Expect = 4.73e-141
Identity = 228/338 (67.46%), Postives = 263/338 (77.81%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTEQR-DVE--------KAAAAKAEEEEELS 62
           MLKLIL+VA+A IF++ ++ +F FRHKL+K+  R D+E        K     A   EEL+
Sbjct: 1   MLKLILIVASATIFVLLLLSIFFFRHKLSKSNHRHDIENPSPDNNQKKQDTTAATVEELT 60

Query: 63  TFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTV 122
           TF+GGEDLT+DEILEAPGEVIGK  YGTLYKASLQ +QR  +L FLRPAA  E S DD V
Sbjct: 61  TFQGGEDLTIDEILEAPGEVIGKYHYGTLYKASLQSTQRATLLMFLRPAAV-ESSVDDVV 120

Query: 123 GFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKIS 182
            FLGSIRH NLVPMLGFYAG RGE+LLVHPFYGRGNL++FITVGE+E  S+ WEIIYKIS
Sbjct: 121 RFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGRGNLSEFITVGESE--SKNWEIIYKIS 180

Query: 183 MGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSA 242
           +GIAKALDHLH  L KP+ HGNLK  NI LD NFEP+VSDFGLHLLLN AA +ELL+ S+
Sbjct: 181 LGIAKALDHLHHGLPKPVPHGNLKPMNIFLDPNFEPYVSDFGLHLLLNAAATRELLQASS 240

Query: 243 DSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDDRI 302
            SGY APEL+KMTDADERTDVYSYGKILLELL   K+  +       GDGGDC VS +R 
Sbjct: 241 ASGYNAPELVKMTDADERTDVYSYGKILLELLS-AKETMNQTAVACGGDGGDCSVSKERN 300

Query: 303 LRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           L+C  +I EACC SSP  RP+FKEVI KIEEIGRI++D
Sbjct: 301 LQCLFQIGEACCCSSPIRRPDFKEVIEKIEEIGRIEMD 334

[Close]

BLAST of MC06g2073 vs. ExPASy TrEMBL
Match: A0A6J1ELS2 (putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777 PE=4 SV=1)

HSP 1 Score: 410 bits (1055), Expect = 7.99e-141
Identity = 230/340 (67.65%), Postives = 266/340 (78.24%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLF--RHKLAKTEQR-DVEKAAAAKAEEEEE-------- 62
           MLK +LLVA+A IF++ +I +F F  RHKL+ TE R DVE+    +  ++E         
Sbjct: 1   MLKFMLLVASAAIFVLLLISIFFFFFRHKLSLTEHRHDVERPCNDEHNKKEHTPPPSPPS 60

Query: 63  LSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDD 122
           L+TFEGGEDLT+DEILEAPGEVIGKS YGTLYKASLQ +Q TR+LRFLRP A   E   D
Sbjct: 61  LATFEGGEDLTIDEILEAPGEVIGKSHYGTLYKASLQSTQATRLLRFLRPGAGGGEY--D 120

Query: 123 TVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYK 182
            V FLGSIRH NLVPMLGFYAG RGE+LLVHPFYG+GNLA+ I   E E+ES+ WE+I K
Sbjct: 121 VVRFLGSIRHRNLVPMLGFYAGGRGERLLVHPFYGQGNLAELIR--ERESESKNWEVINK 180

Query: 183 ISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRV 242
           IS+GIA+ALDHLH+ LQKP+ HGNLKSKNILLD NFEPHVSDFGLHLLLN AA +E+L+V
Sbjct: 181 ISLGIARALDHLHNGLQKPIPHGNLKSKNILLDSNFEPHVSDFGLHLLLNAAATREMLKV 240

Query: 243 SADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGDGGDCGVSDD 302
           SA SGYKAPELI MTDADERTDV+SYGKILLELL   +           GDGGDCG S++
Sbjct: 241 SAASGYKAPELINMTDADERTDVFSYGKILLELLSAEE---------TGGDGGDCGGSEE 300

Query: 303 RILRCS-RIVEACCSSSPDLRPNFKEVIAKIEEIGRIKID 330
           R LRC  +I EACCSSSPD RP+FKEVIAKI EIGRI++D
Sbjct: 301 RTLRCLLQIGEACCSSSPDRRPDFKEVIAKIAEIGRIEMD 327

[Close]

BLAST of MC06g2073 vs. TAIR 10
Match: AT5G07620.1 (Protein kinase superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 5.5e-88
Identity = 181/357 (50.70%), Postives = 236/357 (66.11%), Query Frame = 0


Query: 3   MLKLILLVAAAPIFLIFVILVFLFRHKLAKTE--QRDVEKAAAAK---AEEEEELSTFEG 62
           +L L+L V+ +   ++   ++FL R + + TE  Q DVE     K   + E EEL  F+G
Sbjct: 6   LLTLLLGVSLSAFLIVIFFIIFLRRKESSSTESDQYDVESLDHNKQGFSSETEELVIFQG 65

Query: 63  GEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESGDDTVGF-- 122
           GEDLT+ +IL+APGEVIGKS YGTLYKASLQRS + RVLRFLRP         +  G   
Sbjct: 66  GEDLTICDILDAPGEVIGKSSYGTLYKASLQRSGKIRVLRFLRPVCTVRSDSKEFNGIIE 125

Query: 123 -LGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLADFITVGEAEAESERWEIIYKISM 182
            LG +RH NLVP+LGFYAG RGEKL+VHPF+G GNL+DFI  G  + ES +W  I +I++
Sbjct: 126 TLGFVRHENLVPLLGFYAGNRGEKLMVHPFFGSGNLSDFIRSG--DDESRKWINILRITI 185

Query: 183 GIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAANQELLRVSAD 242
           GI+KALDHLH+ +QKP+ HGNLKSKN+LL  +FEP +SDFGLHLLLN +A QE+L VSA 
Sbjct: 186 GISKALDHLHTGMQKPIVHGNLKSKNVLLSSSFEPRISDFGLHLLLNLSAGQEILDVSAA 245

Query: 243 SGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDGD------------ 302
            GYKAPELIKM D  + +DVYS G I+LE L+ GK +P N N+  D +            
Sbjct: 246 EGYKAPELIKMKDVSKESDVYSLGVIMLE-LVSGK-EPINENATGDDEFYLPDFMRNAVL 305

Query: 303 -------------GGDCGVSDDRILRCSRIVEACCSSSPDLRPNFKEVIAKIEEIGR 327
                        G D  +S++ +L+  ++  +CCS SP LRPN K+V+ K+EEIG+
Sbjct: 306 DHRLSDLYRPEILGSDDNLSEECVLKYFQLAMSCCSPSPSLRPNVKQVLRKLEEIGK 358

[Close]

BLAST of MC06g2073 vs. TAIR 10
Match: AT5G61570.2 (Protein kinase superfamily protein )

HSP 1 Score: 314.7 bits (805), Expect = 8.9e-86
Identity = 184/361 (50.97%), Postives = 239/361 (66.20%), Query Frame = 0


Query: 1   THMLKLILLVAAAPIFLIF--VILVFLF-RHKLAKTE--QRDVEKAAAAK------AEEE 60
           +H++KLIL V+    FLI   +I++F+F R    +T   Q DVE     +      +E E
Sbjct: 4   SHLVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESPYEKQEFSDNGSETE 63

Query: 61  EELSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESG 120
           EEL  F GGEDLT+ +IL+APGEVIGKS YGTLYKA+LQRS + RVLRFLRP  A     
Sbjct: 64  EELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNSDS 123

Query: 121 DDTVGF---LGSIRHPNLVPMLGFYAGRRGEKLLVHPFYG-RGNLADFITVGEAEAESER 180
            +  G    LG +RH NLVP+LGFY G RGEKL++HPF+G  GNL+ FI  G+ +A   +
Sbjct: 124 KEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKCGDVDA--HK 183

Query: 181 WEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAAN 240
           W  I  I++GIAKALDHLH+ +QKP+ HGNLKSKN+LLD++F P VSDFGLHLLLN AA 
Sbjct: 184 WSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNLAAG 243

Query: 241 QELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDG---- 300
           QE+L  SA  GYKAPELIKM +  + +DVYS+G I+LE L+ GK +P+N+N  P G    
Sbjct: 244 QEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLE-LVSGK-EPTNKN--PTGSVLD 303

Query: 301 ------------------DGGDCGVSDDRILRCSRIVEACCSSSPDLRPNFKEVIAKIEE 325
                             DG   GV+++ +L   ++  +CCS SP LRP+FK+V+ K+EE
Sbjct: 304 RNRLSDLYRPEIIRRCLKDGN--GVTEECVLEYFQLAMSCCSPSPTLRPSFKQVLRKLEE 356

[Close]

BLAST of MC06g2073 vs. TAIR 10
Match: AT5G61570.1 (Protein kinase superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 7.5e-85
Identity = 182/362 (50.28%), Postives = 239/362 (66.02%), Query Frame = 0


Query: 1   THMLKLILLVAAAPIFLIF--VILVFLF-RHKLAKTE--QRDVEKAAAAK------AEEE 60
           +H++KLIL V+    FLI   +I++F+F R    +T   Q DVE     +      +E E
Sbjct: 4   SHLVKLILAVSLPTTFLIMIAIIIIFIFCRRTTTETNEVQYDVESPYEKQEFSDNGSETE 63

Query: 61  EELSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRTRVLRFLRPAAATEESG 120
           EEL  F GGEDLT+ +IL+APGEVIGKS YGTLYKA+LQRS + RVLRFLRP  A     
Sbjct: 64  EELIIFNGGEDLTICDILDAPGEVIGKSSYGTLYKATLQRSGKVRVLRFLRPLCAVNSDS 123

Query: 121 DDTVGF---LGSIRHPNLVPMLGFYAGRRGEKLLVHPFYG-RGNLADFIT-VGEAEAESE 180
            +  G    LG +RH NLVP+LGFY G RGEKL++HPF+G  GNL+ FI  +   + ++ 
Sbjct: 124 KEFNGVIESLGFVRHDNLVPLLGFYVGNRGEKLMIHPFFGSSGNLSAFIKFLAGGDVDAH 183

Query: 181 RWEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVSDFGLHLLLNPAA 240
           +W  I  I++GIAKALDHLH+ +QKP+ HGNLKSKN+LLD++F P VSDFGLHLLLN AA
Sbjct: 184 KWSNILSITIGIAKALDHLHTGMQKPIVHGNLKSKNVLLDKSFRPRVSDFGLHLLLNLAA 243

Query: 241 NQELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQPSNRNSPPDG--- 300
            QE+L  SA  GYKAPELIKM +  + +DVYS+G I+LE L+ GK +P+N+N  P G   
Sbjct: 244 GQEVLEASAAEGYKAPELIKMKEVSKESDVYSFGVIMLE-LVSGK-EPTNKN--PTGSVL 303

Query: 301 -------------------DGGDCGVSDDRILRCSRIVEACCSSSPDLRPNFKEVIAKIE 325
                              DG   GV+++ +L   ++  +CCS SP LRP+FK+V+ K+E
Sbjct: 304 DRNRLSDLYRPEIIRRCLKDGN--GVTEECVLEYFQLAMSCCSPSPTLRPSFKQVLRKLE 359

[Close]

BLAST of MC06g2073 vs. TAIR 10
Match: AT3G24660.1 (transmembrane kinase-like 1 )

HSP 1 Score: 184.9 bits (468), Expect = 1.1e-46
Identity = 106/314 (33.76%), Postives = 168/314 (53.50%), Query Frame = 0


Query: 34  EQRDVEKAAAAKAEEEEELSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQRT 93
           E+ D E     K   E +L  F+GGE+LT+D++L A G+V+ K+ YGT+YKA L      
Sbjct: 339 EEGDEEDEIGEKEGGEGKLVVFQGGENLTLDDVLNATGQVMEKTSYGTVYKAKLSDGGNI 398

Query: 94  RVLRFLRPAAATEESGD-DTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLAD 153
             LR LR     + S     +  LG IRH NLVP+  FY G+RGEKLL++ +    +L D
Sbjct: 399 -ALRLLREGTCKDRSSCLPVIRQLGRIRHENLVPLRAFYQGKRGEKLLIYDYLPNISLHD 458

Query: 154 FITVGEAEAESERWEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHVS 213
            +   +    +  W   +KI++GIA+ L +LH+  + P+ HGN++SKN+L+D  F   ++
Sbjct: 459 LLHESKPRKPALNWARRHKIALGIARGLAYLHTGQEVPIIHGNIRSKNVLVDDFFFARLT 518

Query: 214 DFGLHLLLNPAANQELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKDQP 273
           +FGL  ++  A   E++  +   GYKAPEL KM   + R+DVY++G +LLE+L+  K   
Sbjct: 519 EFGLDKIMVQAVADEIVSQAKSDGYKAPELHKMKKCNPRSDVYAFGILLLEILMGKKPGK 578

Query: 274 SNRNSPPDGD-----------------------GGDCGVSDDRILRCSRIVEACCSSSPD 324
           S RN     D                        G     ++ ++   ++   CC+    
Sbjct: 579 SGRNGNEFVDLPSLVKAAVLEETTMEVFDLEAMKGIRSPMEEGLVHALKLAMGCCAPVTT 638

[Close]

BLAST of MC06g2073 vs. TAIR 10
Match: AT3G51740.1 (inflorescence meristem receptor-like kinase 2 )

HSP 1 Score: 162.5 bits (410), Expect = 5.6e-40
Identity = 106/312 (33.97%), Postives = 154/312 (49.36%), Query Frame = 0


Query: 32  KTEQRDVEKAAAAKAEEEEELSTFEGGEDLTVDEILEAPGEVIGKSKYGTLYKASLQRSQ 91
           KT    V   A+A  E   +L  F+G    T D++L A  E++GKS YGT YKA+L+   
Sbjct: 501 KTVSAGVAGTASAGGEMGGKLVHFDGPFVFTADDLLCATAEIMGKSTYGTAYKATLEDGN 560

Query: 92  RTRVLRFLRPAAATEESGDDTVGFLGSIRHPNLVPMLGFYAGRRGEKLLVHPFYGRGNLA 151
              V R         +  +  V  LG IRH NL+ +  +Y G +GEKLLV  +  +G+L+
Sbjct: 561 EVAVKRLREKTTKGVKEFEGEVTALGKIRHQNLLALRAYYLGPKGEKLLVFDYMSKGSLS 620

Query: 152 DFITVGEAEAESERWEIIYKISMGIAKALDHLHSELQKPLAHGNLKSKNILLDRNFEPHV 211
            F+     E     WE   KI+ GI++ L HLHS   + + H NL + NILLD     H+
Sbjct: 621 AFLHARGPETLIP-WETRMKIAKGISRGLAHLHS--NENMIHENLTASNILLDEQTNAHI 680

Query: 212 SDFGLHLLLNPAANQELLRVSADSGYKAPELIKMTDADERTDVYSYGKILLELLLYGKD- 271
           +D+GL  L+  AA   ++  +   GY+APE  K+ +A  +TDVYS G I+LELL  GK  
Sbjct: 681 ADYGLSRLMTAAAATNVIATAGTLGYRAPEFSKIKNASAKTDVYSLGIIILELLT-GKSP 740

Query: 272 -QPSNRNSPPDGDGG-----------------DCGVSDDRILRCSRIVEACCSSSPDLRP 325
            +P+N    P                      +     D +L   ++   C   SP  RP
Sbjct: 741 GEPTNGMDLPQWVASIVKEEWTNEVFDLELMRETQSVGDELLNTLKLALHCVDPSPAARP 800

The following BLAST results are available for this feature:

BLAST of MC06g2073 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P33543	1.5e-45	33.76	Putative kinase-like protein TMKL1 OS=Arabidopsis thaliana OX=3702 GN=TMKL1 PE=1...	[more]
C0LGP9	8.5e-41	32.39	Probable leucine-rich repeat receptor-like protein kinase IMK3 OS=Arabidopsis th...	[more]
Q9SCT4	7.9e-39	33.97	Probably inactive leucine-rich repeat receptor-like protein kinase IMK2 OS=Arabi...	[more]
Q9SUQ3	9.4e-32	30.79	Probable inactive receptor kinase At4g23740 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q9ZRF9	9.7e-29	30.97	Probable LRR receptor-like serine/threonine-protein kinase RPK1 OS=Arabidopsis t...	[more]

BLAST of MC06g2073 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156696.1	2.38e-230	100.00	putative kinase-like protein TMKL1 [Momordica charantia]	[more]
XP_023532398.1	8.92e-144	68.93	putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo]	[more]
XP_023520536.1	5.46e-143	68.13	putative kinase-like protein TMKL1 [Cucurbita pepo subsp. pepo] >XP_023520537.1 ...	[more]
KAG6605638.1	1.47e-142	68.34	putative kinase-like protein TMKL1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022957896.1	2.08e-142	68.34	putative kinase-like protein TMKL1 [Cucurbita moschata]	[more]

BLAST of MC06g2073 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DVQ7	1.15e-230	100.00	putative kinase-like protein TMKL1 OS=Momordica charantia OX=3673 GN=LOC11102353...	[more]
A0A6J1H0H8	1.01e-142	68.34	putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111459294...	[more]
A0A6J1I3Q5	1.16e-142	68.04	putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111468894 P...	[more]
A0A6J1K4E4	4.73e-141	67.46	putative kinase-like protein TMKL1 OS=Cucurbita maxima OX=3661 GN=LOC111490677 P...	[more]
A0A6J1ELS2	7.99e-141	67.65	putative kinase-like protein TMKL1 OS=Cucurbita moschata OX=3662 GN=LOC111435777...	[more]

BLAST of MC06g2073 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07620.1	5.5e-88	50.70	Protein kinase superfamily protein	[more]
AT5G61570.2	8.9e-86	50.97	Protein kinase superfamily protein	[more]
AT5G61570.1	7.5e-85	50.28	Protein kinase superfamily protein	[more]
AT3G24660.1	1.1e-46	33.76	transmembrane kinase-like 1	[more]
AT3G51740.1	5.6e-40	33.97	inflorescence meristem receptor-like kinase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 31..51
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 62..329 e-value: 4.5E-46 score: 159.1
None	No IPR available	PANTHER	PTHR48008:SF13	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 2..273
None	No IPR available	PANTHER	PTHR48008:SF13	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 288..325
None	No IPR available	PANTHER	PTHR48008	LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATED	coord: 2..273
None	No IPR available	PANTHER	PTHR48008	LEUCINE-RICH REPEAT RECEPTOR-LIKE PROTEIN KINASE IMK3-RELATED	coord: 288..325
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 72..320 e-value: 3.9E-25 score: 88.6
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 68..324 score: 25.700571
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 71..322

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g2073.1	MC06g2073.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity