MC06g1896 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1896
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionUPF0496 protein At3g49070
LocationMC06: 26385724 .. 26386791 (-)
RNA-Seq ExpressionMC06g1896
SyntenyMC06g1896
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATGGTGAGCCACATATCACGAGCTATCCAATAGGTGTAGACGTGCGGGAGGAGTATGCTAATGCTTTCCGCACTGAATCCTACATCGACTTTTGGACACGTGTCCTTACCTTAAATGATGGAGATCTCACAACCCAACTTCCAGTGGAGTCCACAACTGCAACCCGACTCTCATCTTATAGGTTATTTGCCGAACATCTATTGGACCCAACCCAACCCACAGTTAAAAGGCTCTTGGCTTCGACCTATCTTAGGCCTAATTCTTACTCACTTCTCACTGATTATTTCTCTCACACAGCCAATGCTTCTCTCTTATGTGGGCGCTTACTTAAAGATATCCATAATTTACGTCTCAAGCTTCGTCCCCTTAAAATTACCCTCCACTCCATTGAAAATACAAGATTTTACCATAATTATAATTTCGAACCAATCTTGACCTGTTTGGTTGAATTTTCCAACGCCCACAACCCATTTGTGTCGTCCGCCCCATCTACTCGACGAATTCGAATAATCCAAAGTGGGTGCTCCAATTTGCTAAAGCAACTCGAGTTCAACCGCGACAAGGCACGGGCCAAGCTCAGGAGAGTTAGGTACTTCCAACACGGCTCGGCTGGTTTCTTGGTGGCTTTAACGACATCGCTTACAGTAATAGTCATGACTCATGGAATTGCATTGTTTGTTGCTGCGCCGGGCCTCCTTGTGGCTTCGATAGAGTTGGCTAAGTCAAGGAAGCTAGCCAAACAAGTGGCTCAACTCAATGTGGCCGCAAAAGGAACTTACACTTTGAATAGGGATTTCGACACAGTTGGTCGACTCGTGGCTCGGCTGAGTCATGAGCTGGAACACATGAGGGTAATGACGAGGTTTTGGCTTGAGAGAGGAGAGGATAGACGCCAAGCCATTAGTGAAGTGGCTCGTCAGTTGAAGCAAAACCATGCAAGCTTTAGCCAACAACTAGATGAACTTGAGGAACATTTGTATTTGTGTTTTATGACCATAAATCGGGCCAGAAATCTTGTCGTAAAAGAGATTCTGGATCCGGGTCAACCCGCTAAGGCTCCACATGTA

mRNA sequence

GATGGTGAGCCACATATCACGAGCTATCCAATAGGTGTAGACGTGCGGGAGGAGTATGCTAATGCTTTCCGCACTGAATCCTACATCGACTTTTGGACACGTGTCCTTACCTTAAATGATGGAGATCTCACAACCCAACTTCCAGTGGAGTCCACAACTGCAACCCGACTCTCATCTTATAGGTTATTTGCCGAACATCTATTGGACCCAACCCAACCCACAGTTAAAAGGCTCTTGGCTTCGACCTATCTTAGGCCTAATTCTTACTCACTTCTCACTGATTATTTCTCTCACACAGCCAATGCTTCTCTCTTATGTGGGCGCTTACTTAAAGATATCCATAATTTACGTCTCAAGCTTCGTCCCCTTAAAATTACCCTCCACTCCATTGAAAATACAAGATTTTACCATAATTATAATTTCGAACCAATCTTGACCTGTTTGGTTGAATTTTCCAACGCCCACAACCCATTTGTGTCGTCCGCCCCATCTACTCGACGAATTCGAATAATCCAAAGTGGGTGCTCCAATTTGCTAAAGCAACTCGAGTTCAACCGCGACAAGGCACGGGCCAAGCTCAGGAGAGTTAGGTACTTCCAACACGGCTCGGCTGGTTTCTTGGTGGCTTTAACGACATCGCTTACAGTAATAGTCATGACTCATGGAATTGCATTGTTTGTTGCTGCGCCGGGCCTCCTTGTGGCTTCGATAGAGTTGGCTAAGTCAAGGAAGCTAGCCAAACAAGTGGCTCAACTCAATGTGGCCGCAAAAGGAACTTACACTTTGAATAGGGATTTCGACACAGTTGGTCGACTCGTGGCTCGGCTGAGTCATGAGCTGGAACACATGAGGGTAATGACGAGGTTTTGGCTTGAGAGAGGAGAGGATAGACGCCAAGCCATTAGTGAAGTGGCTCGTCAGTTGAAGCAAAACCATGCAAGCTTTAGCCAACAACTAGATGAACTTGAGGAACATTTGTATTTGTGTTTTATGACCATAAATCGGGCCAGAAATCTTGTCGTAAAAGAGATTCTGGATCCGGGTCAACCCGCTAAGGCTCCACATGTA

Coding sequence (CDS)

GATGGTGAGCCACATATCACGAGCTATCCAATAGGTGTAGACGTGCGGGAGGAGTATGCTAATGCTTTCCGCACTGAATCCTACATCGACTTTTGGACACGTGTCCTTACCTTAAATGATGGAGATCTCACAACCCAACTTCCAGTGGAGTCCACAACTGCAACCCGACTCTCATCTTATAGGTTATTTGCCGAACATCTATTGGACCCAACCCAACCCACAGTTAAAAGGCTCTTGGCTTCGACCTATCTTAGGCCTAATTCTTACTCACTTCTCACTGATTATTTCTCTCACACAGCCAATGCTTCTCTCTTATGTGGGCGCTTACTTAAAGATATCCATAATTTACGTCTCAAGCTTCGTCCCCTTAAAATTACCCTCCACTCCATTGAAAATACAAGATTTTACCATAATTATAATTTCGAACCAATCTTGACCTGTTTGGTTGAATTTTCCAACGCCCACAACCCATTTGTGTCGTCCGCCCCATCTACTCGACGAATTCGAATAATCCAAAGTGGGTGCTCCAATTTGCTAAAGCAACTCGAGTTCAACCGCGACAAGGCACGGGCCAAGCTCAGGAGAGTTAGGTACTTCCAACACGGCTCGGCTGGTTTCTTGGTGGCTTTAACGACATCGCTTACAGTAATAGTCATGACTCATGGAATTGCATTGTTTGTTGCTGCGCCGGGCCTCCTTGTGGCTTCGATAGAGTTGGCTAAGTCAAGGAAGCTAGCCAAACAAGTGGCTCAACTCAATGTGGCCGCAAAAGGAACTTACACTTTGAATAGGGATTTCGACACAGTTGGTCGACTCGTGGCTCGGCTGAGTCATGAGCTGGAACACATGAGGGTAATGACGAGGTTTTGGCTTGAGAGAGGAGAGGATAGACGCCAAGCCATTAGTGAAGTGGCTCGTCAGTTGAAGCAAAACCATGCAAGCTTTAGCCAACAACTAGATGAACTTGAGGAACATTTGTATTTGTGTTTTATGACCATAAATCGGGCCAGAAATCTTGTCGTAAAAGAGATTCTGGATCCGGGTCAACCCGCTAAGGCTCCACATGTA

Protein sequence

DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAKSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV
Homology
BLAST of MC06g1896 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 9.0e-81
Identity = 176/345 (51.01%), Postives = 230/345 (66.67%), Query Frame = 0

Query: 13  VDVREEYANAFRTESYIDFWTRVLTLND-----GDLTTQLPVE-STTATRLSSYRLFAEH 72
           VDVREEYANAFRTESY  FWTRV+ L+         ++  P+E S+T+ RL SYRLFA +
Sbjct: 69  VDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAHN 128

Query: 73  LLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKIT 132
           LLDP   T+ R+L  + +  ++ +LL+DYF  TANA LLC +LLK+IH+LR K   LK  
Sbjct: 129 LLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKPK 188

Query: 133 LHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEFNR 192
            HS        N+N   ++    E S   +PF+SS     RI++I+SGC  LLK+LE  R
Sbjct: 189 FHS-------ENHNSLALIDQFTEISKWFDPFISSG---SRIQLIRSGCLYLLKRLESRR 248

Query: 193 DKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAKSR-KL 252
           DK RAKL+ +    H S   ++ALTT+L V + +H  ALF+AAP LL +  + A  R KL
Sbjct: 249 DKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKL 308

Query: 253 AKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISEVA 312
            K  A+L+VAAKGTY L+RD DT+ RLV R++ E+ H+R M  FW+ RG  R +   EVA
Sbjct: 309 TKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVA 368

Query: 313 RQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQP 351
           R+LK+   SFS++LDELEEH+YLCFMTINRARNL+VKEILD   P
Sbjct: 369 RELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDP 403

BLAST of MC06g1896 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 8.5e-55
Identity = 146/347 (42.07%), Postives = 196/347 (56.48%), Query Frame = 0

Query: 14  DVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVES-----TTATRLSSYRLFAEHLL 73
           D REEY +AFRTESY DFW RVL +        +P          + RL SYRLFAEHLL
Sbjct: 33  DFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEHLL 92

Query: 74  DPTQPTVKRLLAS---TYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKI 133
           +P Q  V   LAS   + LRP+   LL  Y++ TANAS LC  LLKDI ++RL+ RPLK 
Sbjct: 93  EPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPLKH 152

Query: 134 TLHSIENTRFYHNYNFEPILTCLVEFSNA-HNPFVSSAPSTRRIRIIQSGCSNLLKQLEF 193
           TL  + +         +  ++ L + S A   PF + A S  R+R +Q+G  +LL+ L+ 
Sbjct: 153 TLRKLAS---------DVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDA 212

Query: 194 NRDKARAKLRRVRYFQHG-SAGFL--VALTTSLTVIVMTHGIALFVAAPGLLVASI--EL 253
            R KAR ++R V   +   S  F+  VA+   +   +  H +A F A P +  A +    
Sbjct: 213 GRKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERF 272

Query: 254 AKSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLER-----G 313
              R   + + QL  AAKGTY LNRD +T+ RLVAR+  E EHM  + R  +E       
Sbjct: 273 FSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRPAAGA 332

Query: 314 EDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVV 342
             + + + EV RQL +N  SF QQLDELEEHL+LCFMTIN+AR +V+
Sbjct: 333 GGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVM 370

BLAST of MC06g1896 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.2e-54
Identity = 145/347 (41.79%), Postives = 195/347 (56.20%), Query Frame = 0

Query: 14  DVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVES-----TTATRLSSYRLFAEHLL 73
           D REEY +AFRTESY DFW RVL +        +P          + RL SYRLFAEHLL
Sbjct: 33  DFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEHLL 92

Query: 74  DPTQPTVKRLLAS---TYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKI 133
           +P Q  V   LAS   + LRP+   LL  Y++ TANAS LC  LLKDI ++RL+ RPLK 
Sbjct: 93  EPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPLKH 152

Query: 134 TLHSIENTRFYHNYNFEPILTCLVEFSNA-HNPFVSSAPSTRRIRIIQSGCSNLLKQLEF 193
           TL  + +         +  ++ L + S A   PF + A S  R+R +Q+G  +LL+ L+ 
Sbjct: 153 TLRKLAS---------DVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDA 212

Query: 194 NRDKARAKLRRVRYFQHG-SAGFL--VALTTSLTVIVMTHGIALFVAAPGLLVASI--EL 253
            R KAR ++R V   +   S  F+  VA+   +   +  H +A F A P +  A +    
Sbjct: 213 GRKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERF 272

Query: 254 AKSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLER-----G 313
              R   + + QL  AAKGTY LNRD +T+ RLVAR+  E EHM  + R  +E       
Sbjct: 273 FSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVEHRPAAGA 332

Query: 314 EDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVV 342
             + + + EV RQL +N  SF QQLDELEEHL+LCFMT N+AR +V+
Sbjct: 333 GGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVM 370

BLAST of MC06g1896 vs. ExPASy Swiss-Prot
Match: Q9LJK4 (UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.7e-27
Identity = 103/350 (29.43%), Postives = 175/350 (50.00%), Query Frame = 0

Query: 14  DVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLDPTQP 73
           ++  E A+AF+T SY D  +R+L ++     TQ  +E           LF    L P   
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVID----PTQENLE-----------LFLSQELRPNNE 94

Query: 74  TVKRLLASTYLRPNSY-SLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITL----- 133
           +V+  L+  + +  +  +L++ YF H+ +A+  C  L +++H+ R  L    + L     
Sbjct: 95  SVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFP 154

Query: 134 ---HSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEF 193
              HS  +  F  N  F+  L    +     NPF S  P +   +  Q     L  +L+ 
Sbjct: 155 RDSHSAIDESFC-NLAFDVFL----KLDTFENPFAS--PESHSFQDTQLCFYQLADKLDT 214

Query: 194 NRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAK--- 253
              K+++   RVR   H +AG  + L T++ V+  +     + A P +LV +  L     
Sbjct: 215 RIRKSKS---RVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYL 274

Query: 254 ----SRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDR 313
                +K    + QLNVAAKGT+ LN+D DT+ RLV+RL   +++ +++ R  LERG D 
Sbjct: 275 PHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRD- 334

Query: 314 RQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDP 348
              I E  +QL+++H + + QL+ L +H+   F  +N++R+L++KEIL P
Sbjct: 335 VYTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKEILRP 358

BLAST of MC06g1896 vs. ExPASy Swiss-Prot
Match: Q9LT84 (UPF0496 protein At3g19330 OS=Arabidopsis thaliana OX=3702 GN=At3g19330 PE=2 SV=2)

HSP 1 Score: 117.1 bits (292), Expect = 4.1e-25
Identity = 95/338 (28.11%), Postives = 170/338 (50.30%), Query Frame = 0

Query: 14  DVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLDPTQP 73
           ++  E A+AF+T SY D  +RV  + D    TQ+              L    +L P + 
Sbjct: 46  NLSRELAHAFQTPSYHDVRSRVHVVVD---LTQIHHRLIQ----PDIELLLSQVLQPNKE 105

Query: 74  TVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITLHSI--- 133
            V+  +     +    +L++ YF H+ +A+ LC  L +++H+ R  L    + L +I   
Sbjct: 106 CVQEAIRHV-KQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPG 165

Query: 134 ENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEFNRDKAR 193
           ++         +      ++     NPF  S+P +   R  Q   S L   L+    K+R
Sbjct: 166 DSLPAIDESLCDLAFDVFLKLDTFENPF--SSPESYSFRDTQLCFSQLKHNLDRRLRKSR 225

Query: 194 AKLRRVRYFQHGSAGFLVALTTSL---TVIVMTHGIALFVAAPGLLVAS-IELAKSRKLA 253
           +++R + +   GS+  LVA   ++    V++ +H + + +   G L +  +  +  RK  
Sbjct: 226 SRVRLIHHATAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKEL 285

Query: 254 KQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISEVAR 313
             + QLN A+KGT+ LN+D DT+ RLV+RL   +E+ + + R  LERG D   +I E+ +
Sbjct: 286 TNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRD-VHSIQEILK 345

Query: 314 QLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEI 345
            L+++H   + QL +LE+H+ L F  +N+AR+L++ EI
Sbjct: 346 LLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 372

BLAST of MC06g1896 vs. NCBI nr
Match: XP_022159588.1 (UPF0496 protein At3g49070 [Momordica charantia])

HSP 1 Score: 691 bits (1783), Expect = 3.39e-250
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0

Query: 1   DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSY 60
           DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSY
Sbjct: 18  DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSY 77

Query: 61  RLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKL 120
           RLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKL
Sbjct: 78  RLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKL 137

Query: 121 RPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLK 180
           RPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLK
Sbjct: 138 RPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLK 197

Query: 181 QLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELA 240
           QLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELA
Sbjct: 198 QLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELA 257

Query: 241 KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQA 300
           KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLER EDRRQA
Sbjct: 258 KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERREDRRQA 317

Query: 301 ISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 356
           ISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV
Sbjct: 318 ISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 373

BLAST of MC06g1896 vs. NCBI nr
Match: XP_038875828.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 501 bits (1290), Expect = 3.99e-175
Identity = 260/359 (72.42%), Postives = 302/359 (84.12%), Query Frame = 0

Query: 1   DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD-LTTQLPVESTTATRLSS 60
           DGEPH+TS+ + +DVREEYANAFRTESYIDFWTRVL LN+GD LTTQL VESTTAT LSS
Sbjct: 18  DGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLSS 77

Query: 61  YRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLK 120
           YRLF EHLLDP Q T+KR+L S +L  NS SLL DYFSHTANASL C  LLK I++LRLK
Sbjct: 78  YRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRLK 137

Query: 121 LRPLKITLHSIENT-RFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNL 180
           L PL+ITL S+EN  +F+H  +F+ +L  LVEFSN  NPF+   PS  +++IIQ+GCS L
Sbjct: 138 LHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFI---PSIEQVQIIQNGCSKL 197

Query: 181 LKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIE 240
           LKQLEF+RDKA+ KL+RVRYFQH SAGFLVA+T SLTVI++THGIALFVAAPG LV +I+
Sbjct: 198 LKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAIK 257

Query: 241 LAKS-RKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDR 300
           LA   RKL K VA+LN+ AKGTYTLNRDFDT+GRLVARL+HELEHMRVM RFWL+RGED+
Sbjct: 258 LANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDK 317

Query: 301 RQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 356
           R+AI E+ RQL Q+H +FS QLDELEEHLYLCFMTINRARNLVVKEILD GQP K  ++
Sbjct: 318 RRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL 373

BLAST of MC06g1896 vs. NCBI nr
Match: XP_008436996.1 (PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo])

HSP 1 Score: 464 bits (1193), Expect = 2.08e-160
Identity = 248/359 (69.08%), Postives = 291/359 (81.06%), Query Frame = 0

Query: 1   DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD-LTTQLPVESTTATRLSS 60
           DGE H+T+YP+  DV EEYANAFRTESYIDFWTRV+ LN+GD LT Q+ +ESTTATRLSS
Sbjct: 18  DGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSS 77

Query: 61  YRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTD-YFSHTANASLLCGRLLKDIHNLRL 120
           YRLF EHLLDP QPT+KR+L + +L PNS SLL D YFSHT+NASLLC R+LK I +LRL
Sbjct: 78  YRLFVEHLLDPPQPTIKRMLTA-HLGPNSCSLLLDHYFSHTSNASLLCSRILKHIVHLRL 137

Query: 121 KLRPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNL 180
           KLR L            Y + +F+ +L CLVEFSN   P         +++IIQ+GCS L
Sbjct: 138 KLRSLDQNKQEFN----YDDSHFKQLLVCLVEFSNNSTPNSFVPYCMEQVQIIQNGCSKL 197

Query: 181 LKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIE 240
           LK+LE++RDKA+ KL+RVRYFQH SAGFLVA+T S T+IV+THGIALFVAAPG LV +I+
Sbjct: 198 LKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIALFVAAPGFLVGAIK 257

Query: 241 LAK-SRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDR 300
           L K SRKLAK+VAQLNVAAKGTYTLNRDFDT+GRLVARLSHELEHMRVMT+FWL+RGED+
Sbjct: 258 LVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMRVMTKFWLDRGEDK 317

Query: 301 RQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 356
           R AI E+ RQL Q+H +F+QQLDELEEHLYLCFMTINRARNLVVKEILD   P K  ++
Sbjct: 318 RWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILDSSAPIKISYL 371

BLAST of MC06g1896 vs. NCBI nr
Match: XP_031741820.1 (UPF0496 protein At3g49070 [Cucumis sativus] >KAE8647914.1 hypothetical protein Csa_000030 [Cucumis sativus])

HSP 1 Score: 445 bits (1144), Expect = 5.80e-153
Identity = 242/362 (66.85%), Postives = 289/362 (79.83%), Query Frame = 0

Query: 1   DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD-LTTQLPVESTTATRLSS 60
           DG  H+ +YP+  DV EEYANAFRTESYIDFWTRV+ LN+GD LT Q+ +ESTTATRLSS
Sbjct: 18  DGGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSS 77

Query: 61  YRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTD-YFSHTANASLLCGRLLKDIHNLRL 120
           YRLF EHLLDP QPT+K +L + +L PNS SLL D YFSHTANASLLC R+LK I +LRL
Sbjct: 78  YRLFVEHLLDPPQPTIKTILTA-HLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRL 137

Query: 121 KLRPLKITLHSIENTRFYHNYN---FEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGC 180
           KL       HS++  +   N++   F+ +L  L EFSN   P         +++IIQ+GC
Sbjct: 138 KL-------HSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGC 197

Query: 181 SNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVA 240
           S LLK+LE++RDK R KL+RVRYFQH SAGFLVA+T S TVIV+THGIALFVAAPG LV 
Sbjct: 198 SKLLKRLEYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVG 257

Query: 241 SIELAK-SRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERG 300
           +I+LAK SRKLAK+VAQLNVAAKGTYTLNRDFDT+GRLVARLSHELEHM+VM +FWL++G
Sbjct: 258 AIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDKG 317

Query: 301 EDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAP 356
            D+R AI E+ARQL Q+H +F+QQLDELEEHLYLCFMTINRARNLVVKEIL+  +P K  
Sbjct: 318 GDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKIS 371

BLAST of MC06g1896 vs. NCBI nr
Match: OMO71809.1 (hypothetical protein COLO4_28024 [Corchorus olitorius])

HSP 1 Score: 422 bits (1085), Expect = 7.73e-144
Identity = 223/347 (64.27%), Postives = 268/347 (77.23%), Query Frame = 0

Query: 5   HITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQL-PVESTTATRLSSYRLF 64
           ++   P  VDVREEYANAFRTESY+DFWTRVL +++ +  T + P++STTA RL SYRLF
Sbjct: 22  NLAVIPNSVDVREEYANAFRTESYMDFWTRVLAISNTEFATCISPMDSTTAARLPSYRLF 81

Query: 65  AEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPL 124
           AEHLLDP Q TV R+L     RP ++SLL DYFS TANASLLCG LLK I + RLK R  
Sbjct: 82  AEHLLDPDQSTVTRILNLAQNRPTTHSLLLDYFSQTANASLLCGLLLKVIDHTRLKYRSF 141

Query: 125 KITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLE 184
           + +  ++   +F +   F  I+TCL+EFSN  NPFVSS+PS+ R+RIIQ+GC  LLK+LE
Sbjct: 142 RTSFQALGVVQFSNENQFSGIVTCLIEFSNCPNPFVSSSPSSNRVRIIQAGCCELLKRLE 201

Query: 185 FNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAKSR 244
            +RDKARAKL  V   QHGS  FLVALTTSLT+IV +H +AL V APGL+ AS ELA SR
Sbjct: 202 TSRDKARAKLHLVNSLQHGSGIFLVALTTSLTIIVASHALALLVTAPGLIAASFELASSR 261

Query: 245 KLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISE 304
           +L ++  QL+ AAKGTY LNRD DT+ RLVARL+ E+E MR M +FWLERGEDR QA  E
Sbjct: 262 RLFRESTQLDAAAKGTYILNRDLDTISRLVARLNDEVEDMRAMVKFWLERGEDRLQASCE 321

Query: 305 VARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQP 350
           VAR+LK+N A+F+QQLD+LEEHLYLCFMTINRARNLVVKEILDPG P
Sbjct: 322 VARRLKKNDANFTQQLDDLEEHLYLCFMTINRARNLVVKEILDPGPP 368

BLAST of MC06g1896 vs. ExPASy TrEMBL
Match: A0A6J1DZ81 (UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV=1)

HSP 1 Score: 691 bits (1783), Expect = 1.64e-250
Identity = 355/356 (99.72%), Postives = 355/356 (99.72%), Query Frame = 0

Query: 1   DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSY 60
           DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSY
Sbjct: 18  DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSY 77

Query: 61  RLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKL 120
           RLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKL
Sbjct: 78  RLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKL 137

Query: 121 RPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLK 180
           RPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLK
Sbjct: 138 RPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLK 197

Query: 181 QLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELA 240
           QLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELA
Sbjct: 198 QLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELA 257

Query: 241 KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQA 300
           KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLER EDRRQA
Sbjct: 258 KSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERREDRRQA 317

Query: 301 ISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 356
           ISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV
Sbjct: 318 ISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 373

BLAST of MC06g1896 vs. ExPASy TrEMBL
Match: A0A1S3ATJ2 (UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=3 SV=1)

HSP 1 Score: 464 bits (1193), Expect = 1.01e-160
Identity = 248/359 (69.08%), Postives = 291/359 (81.06%), Query Frame = 0

Query: 1   DGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD-LTTQLPVESTTATRLSS 60
           DGE H+T+YP+  DV EEYANAFRTESYIDFWTRV+ LN+GD LT Q+ +ESTTATRLSS
Sbjct: 18  DGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSS 77

Query: 61  YRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTD-YFSHTANASLLCGRLLKDIHNLRL 120
           YRLF EHLLDP QPT+KR+L + +L PNS SLL D YFSHT+NASLLC R+LK I +LRL
Sbjct: 78  YRLFVEHLLDPPQPTIKRMLTA-HLGPNSCSLLLDHYFSHTSNASLLCSRILKHIVHLRL 137

Query: 121 KLRPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNL 180
           KLR L            Y + +F+ +L CLVEFSN   P         +++IIQ+GCS L
Sbjct: 138 KLRSLDQNKQEFN----YDDSHFKQLLVCLVEFSNNSTPNSFVPYCMEQVQIIQNGCSKL 197

Query: 181 LKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIE 240
           LK+LE++RDKA+ KL+RVRYFQH SAGFLVA+T S T+IV+THGIALFVAAPG LV +I+
Sbjct: 198 LKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIALFVAAPGFLVGAIK 257

Query: 241 LAK-SRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDR 300
           L K SRKLAK+VAQLNVAAKGTYTLNRDFDT+GRLVARLSHELEHMRVMT+FWL+RGED+
Sbjct: 258 LVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMRVMTKFWLDRGEDK 317

Query: 301 RQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 356
           R AI E+ RQL Q+H +F+QQLDELEEHLYLCFMTINRARNLVVKEILD   P K  ++
Sbjct: 318 RWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILDSSAPIKISYL 371

BLAST of MC06g1896 vs. ExPASy TrEMBL
Match: A0A0A0KN20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1)

HSP 1 Score: 442 bits (1138), Expect = 1.35e-152
Identity = 241/361 (66.76%), Postives = 288/361 (79.78%), Query Frame = 0

Query: 2   GEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD-LTTQLPVESTTATRLSSY 61
           G  H+ +YP+  DV EEYANAFRTESYIDFWTRV+ LN+GD LT Q+ +ESTTATRLSSY
Sbjct: 4   GGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSY 63

Query: 62  RLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTD-YFSHTANASLLCGRLLKDIHNLRLK 121
           RLF EHLLDP QPT+K +L + +L PNS SLL D YFSHTANASLLC R+LK I +LRLK
Sbjct: 64  RLFVEHLLDPPQPTIKTILTA-HLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLK 123

Query: 122 LRPLKITLHSIENTRFYHNYN---FEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCS 181
           L       HS++  +   N++   F+ +L  L EFSN   P         +++IIQ+GCS
Sbjct: 124 L-------HSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCS 183

Query: 182 NLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVAS 241
            LLK+LE++RDK R KL+RVRYFQH SAGFLVA+T S TVIV+THGIALFVAAPG LV +
Sbjct: 184 KLLKRLEYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGA 243

Query: 242 IELAK-SRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGE 301
           I+LAK SRKLAK+VAQLNVAAKGTYTLNRDFDT+GRLVARLSHELEHM+VM +FWL++G 
Sbjct: 244 IKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDKGG 303

Query: 302 DRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPH 356
           D+R AI E+ARQL Q+H +F+QQLDELEEHLYLCFMTINRARNLVVKEIL+  +P K  +
Sbjct: 304 DKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISY 356

BLAST of MC06g1896 vs. ExPASy TrEMBL
Match: A0A1R3HN72 (Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_28024 PE=3 SV=1)

HSP 1 Score: 422 bits (1085), Expect = 3.74e-144
Identity = 223/347 (64.27%), Postives = 268/347 (77.23%), Query Frame = 0

Query: 5   HITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQL-PVESTTATRLSSYRLF 64
           ++   P  VDVREEYANAFRTESY+DFWTRVL +++ +  T + P++STTA RL SYRLF
Sbjct: 22  NLAVIPNSVDVREEYANAFRTESYMDFWTRVLAISNTEFATCISPMDSTTAARLPSYRLF 81

Query: 65  AEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPL 124
           AEHLLDP Q TV R+L     RP ++SLL DYFS TANASLLCG LLK I + RLK R  
Sbjct: 82  AEHLLDPDQSTVTRILNLAQNRPTTHSLLLDYFSQTANASLLCGLLLKVIDHTRLKYRSF 141

Query: 125 KITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLE 184
           + +  ++   +F +   F  I+TCL+EFSN  NPFVSS+PS+ R+RIIQ+GC  LLK+LE
Sbjct: 142 RTSFQALGVVQFSNENQFSGIVTCLIEFSNCPNPFVSSSPSSNRVRIIQAGCCELLKRLE 201

Query: 185 FNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAKSR 244
            +RDKARAKL  V   QHGS  FLVALTTSLT+IV +H +AL V APGL+ AS ELA SR
Sbjct: 202 TSRDKARAKLHLVNSLQHGSGIFLVALTTSLTIIVASHALALLVTAPGLIAASFELASSR 261

Query: 245 KLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISE 304
           +L ++  QL+ AAKGTY LNRD DT+ RLVARL+ E+E MR M +FWLERGEDR QA  E
Sbjct: 262 RLFRESTQLDAAAKGTYILNRDLDTISRLVARLNDEVEDMRAMVKFWLERGEDRLQASCE 321

Query: 305 VARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQP 350
           VAR+LK+N A+F+QQLD+LEEHLYLCFMTINRARNLVVKEILDPG P
Sbjct: 322 VARRLKKNDANFTQQLDDLEEHLYLCFMTINRARNLVVKEILDPGPP 368

BLAST of MC06g1896 vs. ExPASy TrEMBL
Match: A0A2N9FAS7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11831 PE=3 SV=1)

HSP 1 Score: 424 bits (1089), Expect = 1.43e-143
Identity = 230/344 (66.86%), Postives = 263/344 (76.45%), Query Frame = 0

Query: 10  PIGVDVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLD 69
           P GVDVREEYANAFRTESYIDFWTRVLTL++ D TT++ VE+TTATRLSSYRLF EHLLD
Sbjct: 119 PTGVDVREEYANAFRTESYIDFWTRVLTLSNEDSTTRIQVEATTATRLSSYRLFVEHLLD 178

Query: 70  PTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITLHS 129
           P QPTV R+LAS   RP   SLL++YF+ TAN SLLCG LLKD+ + R   R LK TL S
Sbjct: 179 PDQPTVARILASACNRPKIQSLLSNYFTQTANVSLLCGLLLKDVDHTRGVFRSLKTTLQS 238

Query: 130 IENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEFNRDKA 189
           +E T+     N   +LT L+E SN  NPFV +A ST R+  IQ+ CS LLKQLE +R+KA
Sbjct: 239 LEKTQLSPVKNSPTLLTRLIELSNFINPFVLTASSTTRVWAIQASCSGLLKQLESSREKA 298

Query: 190 RAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAKSRKLAKQV 249
           RAKLR     + GS  FLVAL TSLTVIV  H +AL VA PGL+ AS+ELA  R+LA+  
Sbjct: 299 RAKLRLANRLKRGSGIFLVALATSLTVIVAVHAMALLVATPGLIAASLELASVRRLARST 358

Query: 250 AQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISEVARQLK 309
           AQL+ AAKGTY LNRD +T+ RLVARLS ELEHMR M +FWLER +DR QA  EVA QLK
Sbjct: 359 AQLDAAAKGTYILNRDLETISRLVARLSDELEHMRAMVKFWLERRQDRLQANGEVAHQLK 418

Query: 310 QNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKA 353
           +N  SFSQQLDELEE LYLCFMTINRARNLVVKEI+DPGQP  A
Sbjct: 419 KNDCSFSQQLDELEERLYLCFMTINRARNLVVKEIMDPGQPTMA 462

BLAST of MC06g1896 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 302.0 bits (772), Expect = 6.4e-82
Identity = 176/345 (51.01%), Postives = 230/345 (66.67%), Query Frame = 0

Query: 13  VDVREEYANAFRTESYIDFWTRVLTLND-----GDLTTQLPVE-STTATRLSSYRLFAEH 72
           VDVREEYANAFRTESY  FWTRV+ L+         ++  P+E S+T+ RL SYRLFA +
Sbjct: 69  VDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAHN 128

Query: 73  LLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKIT 132
           LLDP   T+ R+L  + +  ++ +LL+DYF  TANA LLC +LLK+IH+LR K   LK  
Sbjct: 129 LLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESLKPK 188

Query: 133 LHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEFNR 192
            HS        N+N   ++    E S   +PF+SS     RI++I+SGC  LLK+LE  R
Sbjct: 189 FHS-------ENHNSLALIDQFTEISKWFDPFISSG---SRIQLIRSGCLYLLKRLESRR 248

Query: 193 DKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAKSR-KL 252
           DK RAKL+ +    H S   ++ALTT+L V + +H  ALF+AAP LL +  + A  R KL
Sbjct: 249 DKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKL 308

Query: 253 AKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISEVA 312
            K  A+L+VAAKGTY L+RD DT+ RLV R++ E+ H+R M  FW+ RG  R +   EVA
Sbjct: 309 TKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVA 368

Query: 313 RQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQP 351
           R+LK+   SFS++LDELEEH+YLCFMTINRARNL+VKEILD   P
Sbjct: 369 RELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDP 403

BLAST of MC06g1896 vs. TAIR 10
Match: AT3G19250.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 123.2 bits (308), Expect = 4.1e-28
Identity = 103/350 (29.43%), Postives = 175/350 (50.00%), Query Frame = 0

Query: 14  DVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLDPTQP 73
           ++  E A+AF+T SY D  +R+L ++     TQ  +E           LF    L P   
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVID----PTQENLE-----------LFLSQELRPNNE 94

Query: 74  TVKRLLASTYLRPNSY-SLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITL----- 133
           +V+  L+  + +  +  +L++ YF H+ +A+  C  L +++H+ R  L    + L     
Sbjct: 95  SVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFP 154

Query: 134 ---HSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEF 193
              HS  +  F  N  F+  L    +     NPF S  P +   +  Q     L  +L+ 
Sbjct: 155 RDSHSAIDESFC-NLAFDVFL----KLDTFENPFAS--PESHSFQDTQLCFYQLADKLDT 214

Query: 194 NRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAK--- 253
              K+++   RVR   H +AG  + L T++ V+  +     + A P +LV +  L     
Sbjct: 215 RIRKSKS---RVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYL 274

Query: 254 ----SRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDR 313
                +K    + QLNVAAKGT+ LN+D DT+ RLV+RL   +++ +++ R  LERG D 
Sbjct: 275 PHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRD- 334

Query: 314 RQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDP 348
              I E  +QL+++H + + QL+ L +H+   F  +N++R+L++KEIL P
Sbjct: 335 VYTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKEILRP 358

BLAST of MC06g1896 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 117.1 bits (292), Expect = 2.9e-26
Identity = 95/338 (28.11%), Postives = 170/338 (50.30%), Query Frame = 0

Query: 14  DVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLDPTQP 73
           ++  E A+AF+T SY D  +RV  + D    TQ+              L    +L P + 
Sbjct: 46  NLSRELAHAFQTPSYHDVRSRVHVVVD---LTQIHHRLIQ----PDIELLLSQVLQPNKE 105

Query: 74  TVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITLHSI--- 133
            V+  +     +    +L++ YF H+ +A+ LC  L +++H+ R  L    + L +I   
Sbjct: 106 CVQEAIRHV-KQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPG 165

Query: 134 ENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEFNRDKAR 193
           ++         +      ++     NPF  S+P +   R  Q   S L   L+    K+R
Sbjct: 166 DSLPAIDESLCDLAFDVFLKLDTFENPF--SSPESYSFRDTQLCFSQLKHNLDRRLRKSR 225

Query: 194 AKLRRVRYFQHGSAGFLVALTTSL---TVIVMTHGIALFVAAPGLLVAS-IELAKSRKLA 253
           +++R + +   GS+  LVA   ++    V++ +H + + +   G L +  +  +  RK  
Sbjct: 226 SRVRLIHHATAGSSLCLVAAVVAVAASAVVIASHALPILLVVAGPLCSPYLPHSFKRKEL 285

Query: 254 KQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISEVAR 313
             + QLN A+KGT+ LN+D DT+ RLV+RL   +E+ + + R  LERG D   +I E+ +
Sbjct: 286 TNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRD-VHSIQEILK 345

Query: 314 QLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEI 345
            L+++H   + QL +LE+H+ L F  +N+AR+L++ EI
Sbjct: 346 LLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 372

BLAST of MC06g1896 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 116.7 bits (291), Expect = 3.8e-26
Identity = 102/360 (28.33%), Postives = 170/360 (47.22%), Query Frame = 0

Query: 13  VDVREEYANAFRTESYIDFWTRV---LTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLD 72
           + V EEY  AFRT SY++  T+    L +      +      ++++ LS +  F ++LLD
Sbjct: 34  LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLSFHSHFTDYLLD 93

Query: 73  PTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITLHS 132
           P Q T+  L+  + L     +L+  +F  ++ A  +C  LL+ +  +++    +K  +  
Sbjct: 94  PPQETLDALMQDSSLD----NLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIKRVMKI 153

Query: 133 IENT-RFYHNYNFEPILTC------LVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQL 192
            +            P + C      L  F+   NP +    +  + RI+    S+LL +L
Sbjct: 154 GKRVCNGAKTLECSPEMLCALIFQELSRFAALKNP-LCRIVNEAQFRIVHDANSDLLTKL 213

Query: 193 EFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTV----IVMTHGIALFVAAPGLL-VASI 252
              + + R K+R  ++ +    G+ + +T S  V    I+  H I    AAP LL + S 
Sbjct: 214 TSKKRRIRRKIRFFKFCKK-LGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCSF 273

Query: 253 ELAKSRK-------------LAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMR 312
            L + +K             L K   Q+++AAKG + L  D DT+ RL  RL  E+EH +
Sbjct: 274 CLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 333

Query: 313 VMTRFWLERGEDRRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEI 345
            +     +    + + + E  R+   +   FS QL ELEEHLYLCF TINR+R LV+ +I
Sbjct: 334 TVAAMCAK--SRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 385

BLAST of MC06g1896 vs. TAIR 10
Match: AT3G19330.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 104.4 bits (259), Expect = 2.0e-22
Identity = 98/347 (28.24%), Postives = 158/347 (45.53%), Query Frame = 0

Query: 14  DVREEYANAFRTESYIDFWTRVLTLNDGDLTTQLPVESTTATRLSSYRLFAEHLLDPTQP 73
           ++  E A+AF+T SY D  +RV  + D    TQ+              L    +L P + 
Sbjct: 46  NLSRELAHAFQTPSYHDVRSRVHVVVD---LTQIHHRLIQ----PDIELLLSQVLQPNKE 105

Query: 74  TVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRLKLRPLKITLHSI--- 133
            V+  +     +    +L++ YF H+ +A+ LC  L +++H+ R  L    + L +I   
Sbjct: 106 CVQEAIRHV-KQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPG 165

Query: 134 ENTRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSNLLKQLEFNRDKAR 193
           ++         +      ++     NPF  S+P +   R  Q        QL+ N D+  
Sbjct: 166 DSLPAIDESLCDLAFDVFLKLDTFENPF--SSPESYSFRDTQL----CFSQLKHNLDRRL 225

Query: 194 AKLR-RVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASIELAKSRKLAKQV 253
            K R RVR   H +AG        L    + H                     RK    +
Sbjct: 226 RKSRSRVRLIHHATAG-------PLCSPYLPHSF------------------KRKELTNI 285

Query: 254 AQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERGEDRRQAISEVARQLK 313
            QLN A+KGT+ LN+D DT+ RLV+RL   +E+ + + R  LERG D   +I E+ + L+
Sbjct: 286 CQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRD-VHSIQEILKLLR 345

Query: 314 QNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 357
           ++H   + QL +LE+H+ L F  +N+AR+L++ EI  P      PHV
Sbjct: 346 KSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEIHLP-----RPHV 347

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMU49.0e-8151.01UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1[more]
A2XCJ18.5e-5542.07UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2[more]
Q10RR93.2e-5441.79UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 ... [more]
Q9LJK45.7e-2729.43UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1[more]
Q9LT844.1e-2528.11UPF0496 protein At3g19330 OS=Arabidopsis thaliana OX=3702 GN=At3g19330 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_022159588.13.39e-25099.72UPF0496 protein At3g49070 [Momordica charantia][more]
XP_038875828.13.99e-17572.42UPF0496 protein At3g49070-like [Benincasa hispida][more]
XP_008436996.12.08e-16069.08PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo][more]
XP_031741820.15.80e-15366.85UPF0496 protein At3g49070 [Cucumis sativus] >KAE8647914.1 hypothetical protein C... [more]
OMO71809.17.73e-14464.27hypothetical protein COLO4_28024 [Corchorus olitorius][more]
Match NameE-valueIdentityDescription
A0A6J1DZ811.64e-25099.72UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV... [more]
A0A1S3ATJ21.01e-16069.08UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=... [more]
A0A0A0KN201.35e-15266.76Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1[more]
A0A1R3HN723.74e-14464.27Uncharacterized protein OS=Corchorus olitorius OX=93759 GN=COLO4_28024 PE=3 SV=1[more]
A0A2N9FAS71.43e-14366.86Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS11831 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G49070.16.4e-8251.01Protein of unknown function (DUF677) [more]
AT3G19250.14.1e-2829.43Protein of unknown function (DUF677) [more]
AT3G19330.12.9e-2628.11Protein of unknown function (DUF677) [more]
AT1G20180.13.8e-2628.33Protein of unknown function (DUF677) [more]
AT3G19330.22.0e-2228.24Protein of unknown function (DUF677) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 291..325
NoneNo IPR availablePANTHERPTHR31113:SF6UPF0496 PROTEIN 3coord: 12..349
IPR007749Protein of unknown function DUF677PFAMPF05055DUF677coord: 52..344
e-value: 5.5E-13
score: 48.5
IPR007749Protein of unknown function DUF677PANTHERPTHR31113UPF0496 PROTEIN 3-RELATEDcoord: 12..349

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1896.1MC06g1896.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane