MC06g1884 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1884
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPectin lyase-like superfamily protein
LocationMC06: 26222972 .. 26227156 (-)
RNA-Seq ExpressionMC06g1884
SyntenyMC06g1884
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAATGAGTTTTCCTTAGACGTTATATTTACGACCGTTTTACTCTTACATTCTTATGTAAATTCAACGTTTCCATATATATATATATATTTTTTTTTCTTTATTATTTTATTAATTTTTATTTACATTTTTAGTTCGAAATTTCTCCTATAAATTCTTGGTTCTCTCGAATCTCAAAAATCAGAAACTCAAAAAAATGGATCTTCCTCGAAAACCCACACAAGCCCATGTAATTTGTTCTCTCTCTCCTCTCTCTAAATTTCATATGTTTTTTTTTTTTTAGTAGTTGTTTTGTTTTCTTTTTTCCCACTAATTTTTCATATGAGTTCATAAAATTGTTGGCTGGTTTTGGTTCTGACGAACCCCAAATGGTTTTCTGAGGAAATTTTTTGTAGATCGTTCATTTTCTCGGGAAAATTCAAAAACCAACGCAACTGATACAAAGACGGATTCATGAACCCAACAACCGTTTTTTTCCTAATCTGATTAATTTTGTTTCTGATTTTGTTATTGGGAAAAACCCACCAGTTTTCTCGGGAAGCAAACAGAAACAGAGCAAGTTTCATTTAAACCCATTAAATTTTCCGAGAAACAAACAGAAAATTAAAATAGAATCAATTAATGTTGTTTTTCGGGCAGGTTTCGGCGGCCGTCATTCTATTGGCATTGGTGAGTTTAGCAACCGTCGATGGCAGAAAACCGTGTCCAAATTCTAACTGCGAGGAGTTTCCGGCGATTAATTGCCGGAAGTACTCGGCGGTGCTGACGGAGTTCGGCGCCGTCGGCGACGGAGTTACTTCAAATACGCACGCGTTCCGGCAAGCGATCGAGCATCTGAAGCCGCTGGCGGCGGCCGGCGGCGCTCAGCTGTTTGTTCCGGCGGGGAAATGGCTCACCGGAAGCTTTAATCTCACCAGCCATTTCACTCTCTTCCTCCATAAGGATGCTCAAATTCTAGCTTCTCAGGTTTCCCCTTCATCTTTATATATATTGAAATGGAAATATTTATATATTTTTAAGTCATTTTAATTTCACCTTTTTTAATTTGTCACAAGATATCGATTTTTTTACCCATTATTCAAAATTTCGTGAACTAAAAAAGAGGTAAAACAGTTCAAAACGAATCACATGGATATCCACTTCATTATTTTGGAAATTTTTTATTTAGTATCACCTAAAAAAAATAAAAGTGGGAAAAAAATAGCACATGGATACCCTTATTCCTTTGGTGTAATTAAAGAATAAAAAGAGGATAAAGAAAAGTACATGGAATGAAGTTTGAAATGTTTTAATAGTATGAACTAAAAAGGGTGATTTGGAAAGAATTAAAAAGTTGGAGGGTGTATTTGAGAATTTGTAATAAATTTGTTGCATAATGAAATATTAATTTGTAGTCTTCGGTTTGATGTGGGGGTTTTTTTTGGGTATTATATATTTCGAAGTGATTTCATGTTATTAGGCTATCTCTTTTTTAATTAGGTAGAAGTGCTTAAAAAGTGACATTAATTATTTTTCTGAAATGGAAATAAATGTTTATTTAGTGAGTGATATATTAAATATGGTGAGGTAGATATTGACTTCATTGATAGAGTCAAAATTAGTTCAATTTGGAAGAGAGAGAAAAAAAAGTGCGTTTGGAATTTTAATTCTGATTTATTCCGACCCGACCCGACCCAATGGGGTACTGATCGACTGCGGGCGAGAGTATTGAGAACTGATTGGAATTGGAAAATCGGACGGTGCAGGATGAATCAGAATGGCCACTCGTGGCAATTCTTCCTTCTTATGGAAGGGGAAGAGACGCGCCTGGTGGACGCTTCAGCAGTCTCATTTATGGAACCAATCTCACCGATGTTGTCATCACAGGTGATTACTTCTCTCTATTTTTCTTTTTCTATTTCTCTAAATTTAAAAATTCGTTCACCAAACCATTTTTTACGACCTAACTTGTGAAAATTAAAAAACAAAAAATGGCAGTTTTCAAAAAGAGTTTTTTTTTTCTTTTTATTTTTAAAGTTTGACTAGAACTAATGTATAGCATGCAAACTTAATCTTAAAATTTTGAAAATTAGAAACTTTGTGAATTTGTTGACCTCCTCCTCCTTCTTCTTCTTCTTTTTTTTTTTTTACTTGGTTGGATAATTACTTCATGATTGATTTTAGGTTTAAAAATTTATGCATTTTTTTCGCAATTTTAAAATAAACAAAAAAGAAGTAAATAAATATATATTTTAAAAGCATAAAACCAAGCCTTGGTTGTGTTAAATACCTTGAAATGTTATGCATTCGTTTGTTTTATTATATAGTTTTTAAAAATATTTTCAAAATCTAAATAAAAAAATTCAAATGCAAAAAATAACCAATAAAATCGTAAAAAAACATGTAAAAAATTTTAAAATTTTGAAGCTAAAAATGAAATGGCAATTGATAATGAAATTTATAAAAGAGGTAAAAAAAGTGGGTCCCACTCATTGTATCGGTGACGTGGTGTAGGGAACAACGGGACGATTGACGGGCAGGGATCTTATTGGTGGGATAAATTCCACAAAAACGAGCTCAACGTAACCCGGCCGTACCTGATCGAGATCATGTACTCCGATCAGATCCAGATCTCTAATCTCACGTTGGTCAACTCTCCTTCTTGGTTCGTCCATCCGATCTACAGCAGGTTTGACTTTTTACCTTCATCGCACGGCTCCTATTCCTTCCCATTTATTTATTTTATTTTATTTTATCATGTTCTGTTCATTACATTGTTTCCAGCTCTATACTCTCACATCATTAATCTTGTTTCAATAAGATCTCATCAGTTTATTTAAAAGTAGAATACTAAAATTTTGAAAAATAGTACTCTTTTTTAATTTTTTTTTAATTCATTTTGATCTATTTCGTTCAATTTTGTTGGTTCAAAGGGTTTGGTCAATTTCTAAGTTTGGATTGAGGTTATAGGTTCAAAATCCAGATTAGTTTTTTTCTTTACCAACACTAATACTTTTGAAAATAAATTTGTAATTAACATTAACTATTTTTTTAAAGAACTAATTTGAGGTATACAAAAATGAAAAATATGATACTTTTTTTATGGTATTTGGTATGAAATAAACACAATGTAATGTAAAATATTTAGGTCCTGCCTTTAATAAGTGCTGACTATATATTATGTTCAAAATTTCAATAGTGAATTTTCCTATCCTATATTCTAAAAAAAAATTGTAAAATTAATATTGATTTTATGCAAGTGTAATTTCTTAAGAAAAAGAGTAAAAAGAAAATGTGTTTTTTTTTTAATGAATTAATTTTGTGGTGGTGAAAATGCAGTAATGTGATAATTCAAGGGCTCACAATTCTGGCTCCAATCGACTCTCCCAACACGGACGGAATAGACCCAGGTTCATTCAATTTCCAAAATTATGGCTTAGGGTTAAGGTTTGTTTTTTCTTCTTCTTCCTCAATCCTCATCCCTAATTTCTCGATCCAATTCCAGATTCCTGCACCAACACACGAATCGAGGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAATACGGGATCCGATTCGGGATGCCGACCGAGCACCTCCTCATCCGCCGCCTCACCTGCATCTCCCCTGACAGCGCCGGAATCGCCCTCGGCAGCGAAATGTCCGGTGGAATCCGCGACGTCAGGATCGAGGACGTCACCGCCATCAACACTCAGTCCGCCGTCCGGATCAAGACCGCGCGTGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGCCGGATGTTCCTCTCCACCATGAAGTACGTCTTCTGGATGACCGGAGACTACAAATCGCATCCGGATCCCGAGTTCGACCCGGCCGCCATTCCCGAGATTAAGAACATCAATTACAGAGACGTGGTGGCGGAGAACGTGACGTACGCCGCCAGATTGGAGGGCATCTCCGGAGATCCCTTCACCGACATCTGTGTTACCAACGTCACCATCGGCCTCACCAAGAAGCCGAAGAAGTTGCAGTGGAACTGTACGAATGTTGAGGGATTCAGTAGCGGTGTGGTGCCGCCGCCGTGCGCTCCGCTTGCCAAGCCGGCGGAAGGCGTCGGCTGTGCTTTCCCGACAGATCGGCTGCCGATCGAGGAGTTTCCGTTGAAGACCTGCAGTATTTCAACTTGTTCGTTC

mRNA sequence

TTTAATGAGTTTTCCTTAGACGTTATATTTACGACCGTTTTACTCTTACATTCTTATGTAAATTCAACGTTTCCATATATATATATATATTTTTTTTTCTTTATTATTTTATTAATTTTTATTTACATTTTTAGTTCGAAATTTCTCCTATAAATTCTTGGTTCTCTCGAATCTCAAAAATCAGAAACTCAAAAAAATGGATCTTCCTCGAAAACCCACACAAGCCCATGTTTCGGCGGCCGTCATTCTATTGGCATTGGTGAGTTTAGCAACCGTCGATGGCAGAAAACCGTGTCCAAATTCTAACTGCGAGGAGTTTCCGGCGATTAATTGCCGGAAGTACTCGGCGGTGCTGACGGAGTTCGGCGCCGTCGGCGACGGAGTTACTTCAAATACGCACGCGTTCCGGCAAGCGATCGAGCATCTGAAGCCGCTGGCGGCGGCCGGCGGCGCTCAGCTGTTTGTTCCGGCGGGGAAATGGCTCACCGGAAGCTTTAATCTCACCAGCCATTTCACTCTCTTCCTCCATAAGGATGCTCAAATTCTAGCTTCTCAGGATGAATCAGAATGGCCACTCGTGGCAATTCTTCCTTCTTATGGAAGGGGAAGAGACGCGCCTGGTGGACGCTTCAGCAGTCTCATTTATGGAACCAATCTCACCGATGTTGTCATCACAGGGAACAACGGGACGATTGACGGGCAGGGATCTTATTGGTGGGATAAATTCCACAAAAACGAGCTCAACGTAACCCGGCCGTACCTGATCGAGATCATGTACTCCGATCAGATCCAGATCTCTAATCTCACGTTGGTCAACTCTCCTTCTTGGTTCGTCCATCCGATCTACAGCAGTAATGTGATAATTCAAGGGCTCACAATTCTGGCTCCAATCGACTCTCCCAACACGGACGGAATAGACCCAGGTTCATTCAATTTCCAAAATTATGGCTTAGGGTTAAGGTTTGTTTTTTCTTCTTCTTCCTCAATCCTCATCCCTAATTTCTCGATCCAATTCGATTCCTGCACCAACACACGAATCGAGGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAATACGGGATCCGATTCGGGATGCCGACCGAGCACCTCCTCATCCGCCGCCTCACCTGCATCTCCCCTGACAGCGCCGGAATCGCCCTCGGCAGCGAAATGTCCGGTGGAATCCGCGACGTCAGGATCGAGGACGTCACCGCCATCAACACTCAGTCCGCCGTCCGGATCAAGACCGCGCGTGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGCCGGATGTTCCTCTCCACCATGAAGTACGTCTTCTGGATGACCGGAGACTACAAATCGCATCCGGATCCCGAGTTCGACCCGGCCGCCATTCCCGAGATTAAGAACATCAATTACAGAGACGTGGTGGCGGAGAACGTGACGTACGCCGCCAGATTGGAGGGCATCTCCGGAGATCCCTTCACCGACATCTGTGTTACCAACGTCACCATCGGCCTCACCAAGAAGCCGAAGAAGTTGCAGTGGAACTGTACGAATGTTGAGGGATTCAGTAGCGGTGTGGTGCCGCCGCCGTGCGCTCCGCTTGCCAAGCCGGCGGAAGGCGTCGGCTGTGCTTTCCCGACAGATCGGCTGCCGATCGAGGAGTTTCCGTTGAAGACCTGCAGTATTTCAACTTGTTCGTTC

Coding sequence (CDS)

ATGGATCTTCCTCGAAAACCCACACAAGCCCATGTTTCGGCGGCCGTCATTCTATTGGCATTGGTGAGTTTAGCAACCGTCGATGGCAGAAAACCGTGTCCAAATTCTAACTGCGAGGAGTTTCCGGCGATTAATTGCCGGAAGTACTCGGCGGTGCTGACGGAGTTCGGCGCCGTCGGCGACGGAGTTACTTCAAATACGCACGCGTTCCGGCAAGCGATCGAGCATCTGAAGCCGCTGGCGGCGGCCGGCGGCGCTCAGCTGTTTGTTCCGGCGGGGAAATGGCTCACCGGAAGCTTTAATCTCACCAGCCATTTCACTCTCTTCCTCCATAAGGATGCTCAAATTCTAGCTTCTCAGGATGAATCAGAATGGCCACTCGTGGCAATTCTTCCTTCTTATGGAAGGGGAAGAGACGCGCCTGGTGGACGCTTCAGCAGTCTCATTTATGGAACCAATCTCACCGATGTTGTCATCACAGGGAACAACGGGACGATTGACGGGCAGGGATCTTATTGGTGGGATAAATTCCACAAAAACGAGCTCAACGTAACCCGGCCGTACCTGATCGAGATCATGTACTCCGATCAGATCCAGATCTCTAATCTCACGTTGGTCAACTCTCCTTCTTGGTTCGTCCATCCGATCTACAGCAGTAATGTGATAATTCAAGGGCTCACAATTCTGGCTCCAATCGACTCTCCCAACACGGACGGAATAGACCCAGGTTCATTCAATTTCCAAAATTATGGCTTAGGGTTAAGGTTTGTTTTTTCTTCTTCTTCCTCAATCCTCATCCCTAATTTCTCGATCCAATTCGATTCCTGCACCAACACACGAATCGAGGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAATACGGGATCCGATTCGGGATGCCGACCGAGCACCTCCTCATCCGCCGCCTCACCTGCATCTCCCCTGACAGCGCCGGAATCGCCCTCGGCAGCGAAATGTCCGGTGGAATCCGCGACGTCAGGATCGAGGACGTCACCGCCATCAACACTCAGTCCGCCGTCCGGATCAAGACCGCGCGTGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGCCGGATGTTCCTCTCCACCATGAAGTACGTCTTCTGGATGACCGGAGACTACAAATCGCATCCGGATCCCGAGTTCGACCCGGCCGCCATTCCCGAGATTAAGAACATCAATTACAGAGACGTGGTGGCGGAGAACGTGACGTACGCCGCCAGATTGGAGGGCATCTCCGGAGATCCCTTCACCGACATCTGTGTTACCAACGTCACCATCGGCCTCACCAAGAAGCCGAAGAAGTTGCAGTGGAACTGTACGAATGTTGAGGGATTCAGTAGCGGTGTGGTGCCGCCGCCGTGCGCTCCGCTTGCCAAGCCGGCGGAAGGCGTCGGCTGTGCTTTCCCGACAGATCGGCTGCCGATCGAGGAGTTTCCGTTGAAGACCTGCAGTATTTCAACTTGTTCGTTC

Protein sequence

MDLPRKPTQAHVSAAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAKPAEGVGCAFPTDRLPIEEFPLKTCSISTCSF
Homology
BLAST of MC06g1884 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 7.6e-174
Identity = 298/492 (60.57%), Postives = 362/492 (73.58%), Query Frame = 0

Query: 12  VSAAVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAFR 71
           VS  V ++ L+S++  + RK        E+ AI+CR +SA L +FG VGDG T NT AF+
Sbjct: 25  VSVLVAVVLLLSVSRGECRKG-RILEALEYSAISCRAHSASLVDFGGVGDGQTLNTKAFQ 84

Query: 72  QAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAIL 131
            A+  L    + GGAQL+VPAGKWLTGSF+LTSHFTLFLH+DA +LASQD S+WP++  L
Sbjct: 85  DAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHFTLFLHRDAVLLASQDISQWPVIKPL 144

Query: 132 PSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIE 191
           PSYGRGRDA  GR++SLI+GTNLTDV+ITG+NGTIDGQG  WW +FH  +L  TRPYLIE
Sbjct: 145 PSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIE 204

Query: 192 IMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPGSFNFQNYG 251
           +MYS  IQISNLTL+NSPSW VHP+YS N++IQG+TILAP+ SPNTDGI+P         
Sbjct: 205 LMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGITILAPVRSPNTDGINP--------- 264

Query: 252 LGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTE 311
                                 DSCTNTRIEDC+IVSGDDC+AVKSGWD+YGI +GMPT+
Sbjct: 265 ----------------------DSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTK 324

Query: 312 HLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDIF 371
            L+IRRLTCISP SA IALGSEMSGGI+DVR ED+ AIN++S +RIKT  GRGG+VKDI+
Sbjct: 325 QLVIRRLTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIY 384

Query: 372 VRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISGD 431
           VR M + TMK+ FWMTG+Y SH D  +DP A P I+ INYRD+VAENV+ AARLEGI  D
Sbjct: 385 VRGMTMKTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSD 444

Query: 432 PFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK--PAEGVGCAFPTDR 491
           PFT IC++NVTI L  K KK+ W CT+VEG SSGV P PC+ L    P +   C FP + 
Sbjct: 445 PFTGICISNVTIHLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCNFPAES 484

Query: 492 LPIEEFPLKTCS 502
           LPI+   L+ CS
Sbjct: 505 LPIDTVELQKCS 484

BLAST of MC06g1884 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 3.1e-26
Identity = 117/434 (26.96%), Postives = 180/434 (41.47%), Query Frame = 0

Query: 53  LTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKW-------LTGSFNLTSH 112
           +T+FGAVGDGVT ++ AF +A E +   +  G  Q  VPAG           GS   T  
Sbjct: 26  VTQFGAVGDGVTDDSQAFLKAWEAV--CSGTGDGQFVVPAGMTFMLQPLKFQGSCKSTPV 85

Query: 113 FTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGT 172
           F   L K                 + PS G  +    G     I  T++  +VI G +G 
Sbjct: 86  FVQMLGK----------------LVAPSKGNWK----GDKDQWILFTDIEGLVIEG-DGE 145

Query: 173 IDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQG 232
           I+GQGS WW+  HK     +RP  ++    + +++S LT ++SP   +H    + V I  
Sbjct: 146 INGQGSSWWE--HKG----SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISS 205

Query: 233 LTILAPIDSPNTDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCF 292
           L I AP  SPNTDGID G                               + +N  I+DC 
Sbjct: 206 LRINAPESSPNTDGIDVG-------------------------------ASSNVVIQDCI 265

Query: 293 IVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGG----IRDV 352
           I +GDDCIA+ SG           T ++ I  + C      GI++GS    G    + +V
Sbjct: 266 IATGDDCIAINSG-----------TSNIHISGIDC--GPGHGISIGSLGKDGETATVENV 325

Query: 353 RIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKY-----VFWMTGDYKSHPDP 412
            +++     T +  RIKT +G  G+ + I    + L  ++       F+  GD  +  D 
Sbjct: 326 CVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDR 384

Query: 413 EFDPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTI--GLTKKPKKLQW 469
           +     + ++   N+  +      Y          P T+I + ++ I    +   +  Q 
Sbjct: 386 KSSAVEVSKVVFSNF--IGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQG 384

BLAST of MC06g1884 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.6e-25
Identity = 123/439 (28.02%), Postives = 177/439 (40.32%), Query Frame = 0

Query: 55  EFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS---FNLTSHFTLFLH 114
           ++GAVGDG   +T AF               A L VPA K    +   F       L   
Sbjct: 63  QYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLSFK 122

Query: 115 KDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGS 174
            D  I+A  D + W    I   + +                 LTD  + G  G IDGQG 
Sbjct: 123 VDGTIVAQPDPARWKNSKIWLQFAQ-----------------LTDFNLMG-TGVIDGQGQ 182

Query: 175 YWWDKFHK--------NELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVII 234
            WW    K        N+ N  RP  I+I YS  + +  LTL+NSP + +       V I
Sbjct: 183 QWWAGQCKVVNGRTVCNDRN--RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKI 242

Query: 235 QGLTILAPIDSPNTDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIED 294
           QGL I AP DSPNTDGID               +F+S                    IE 
Sbjct: 243 QGLKIKAPRDSPNTDGID---------------IFASK----------------RFHIEK 302

Query: 295 CFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGS----EMSGGIR 354
           C I +GDDCIA+ +G           + ++ I+ L C      GI++GS         + 
Sbjct: 303 CVIGTGDDCIAIGTG-----------SSNITIKDLIC--GPGHGISIGSLGRDNSRAEVS 362

Query: 355 DVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFD 414
            V +     I+TQ+ +RIKT +G  G    I    + +   +    +   Y +      +
Sbjct: 363 HVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQN 422

Query: 415 PAAIPEIKNINYRDVVAENVTYAA-RLEGISGDPFTDICVTNVTIGLTK-KPKKLQWNCT 474
             +  +I+ + Y+++   + T AA +L      P T I ++NV++ LT  KP     +C 
Sbjct: 423 QRSAVQIQGVTYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPA----SCV 430

BLAST of MC06g1884 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 107.8 bits (268), Expect = 3.6e-22
Identity = 106/430 (24.65%), Postives = 174/430 (40.47%), Query Frame = 0

Query: 53  LTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGK-WLTGSFNLTS--HFTLF 112
           +++FGA GDG T +T AF  A +  K  ++ G   L VP G  +L  S  LT   +  L 
Sbjct: 70  VSDFGAKGDGKTDDTQAFVNAWK--KACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILT 129

Query: 113 LHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQ 172
           +     + ASQ  S++  ++              ++       NL+  V  G+ G +DG 
Sbjct: 130 VQIFGTLSASQKRSDYKDIS--------------KWIMFDGVNNLS--VDGGDTGVVDGN 189

Query: 173 GSYWWD---KFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGL 232
           G  WW    K +K +     P  +    S  + + NL + N+    +     SNV +  +
Sbjct: 190 GETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNV 249

Query: 233 TILAPIDSPNTDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFI 292
            + AP DSPNTDG                               I   +  N R+ +  I
Sbjct: 250 VVTAPADSPNTDG-------------------------------IHITNTQNIRVSESII 309

Query: 293 VSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGS----EMSGGIRDVR 352
            +GDDCI+++SG           ++++ I  +TC      GI++GS         +  V 
Sbjct: 310 GTGDDCISIESG-----------SQNVQINDITC--GPGHGISIGSLGDDNSKAFVSGVT 369

Query: 353 IEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAA 412
           ++      T + VRIKT +G  G   +I  + + +  +K    +  DY        + +A
Sbjct: 370 VDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSA 429

Query: 413 IPEIKNINYRDVVAENVTYAARLEGISGD-PFTDICVTNVTIGLTKKPKKLQWNCTNVEG 472
           + ++KN+ YRD+   + +  A     S + P   I +  V I      K  +  CTN   
Sbjct: 430 V-QVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI------KGGKATCTNANV 430

BLAST of MC06g1884 vs. ExPASy Swiss-Prot
Match: P27644 (Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.3e-21
Identity = 75/252 (29.76%), Postives = 107/252 (42.46%), Query Frame = 0

Query: 190 IEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPGSFNFQN 249
           + ++   + Q+   T+ N+ SW +HP    ++     TI+AP DSPNTDG +P       
Sbjct: 25  LHLVSCHKTQLLGFTIRNAASWTIHPQGCEDLTAAASTIIAPHDSPNTDGFNP------- 84

Query: 250 YGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVKSGW-------DQY 309
                                   +SC N  I       GDDCIAVK+G        D  
Sbjct: 85  ------------------------ESCRNVMISGVRFSVGDDCIAVKAGKRGPDGEDDHL 144

Query: 310 GIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKT-AR 369
               G+   H L++      P   G+ +GSEMSGG+ DV +ED   I T   +R+KT AR
Sbjct: 145 AETRGITVRHCLMQ------PGHGGLVIGSEMSGGVHDVTVEDCDMIGTDRGLRLKTGAR 204

Query: 370 GRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFD------PAAI----PEIKNINY 424
             GG V +I +RR+ L  ++        Y    D   D      PA +    P +  I  
Sbjct: 205 SGGGMVGNITMRRVLLDGVQTALSANAHYHCDADGHDDWVQSRNPAPVNDGTPFVDGITV 239

BLAST of MC06g1884 vs. NCBI nr
Match: XP_038906749.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 821 bits (2120), Expect = 3.53e-297
Identity = 402/506 (79.45%), Postives = 438/506 (86.56%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP+  T+ HV+AA    VI+LALVSLATV+G+KPCP +N  E PAI+CRK++AVLT+F
Sbjct: 1   MDLPKNSTKFHVTAARISAVIILALVSLATVEGKKPCPTTNYLEVPAISCRKHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNTHAFRQAIEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HK A I
Sbjct: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKYATI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLVA+LPSYGRGRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHK+ELNVTRPYLIE+MYSDQIQIS+LTLVNSPSWFVHPIYS NVII+GLTILAPIDSPN
Sbjct: 181 FHKDELNVTRPYLIEMMYSDQIQISDLTLVNSPSWFVHPIYSRNVIIRGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC+NTRIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCSNTRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE+VT INTQSAVR
Sbjct: 301 SGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTGINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDP A+PEI NINYRDVVA
Sbjct: 361 IKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPTAVPEITNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARLEGISGDPFTDIC++NV IGLT KPKKLQWNCT+VEGFSS V+PPPCAPLAK
Sbjct: 421 ENVTYSARLEGISGDPFTDICISNVKIGLTAKPKKLQWNCTDVEGFSSDVMPPPCAPLAK 475

Query: 481 PAEGVGCAFPTDRLPIEEFPLKTCSI 502
            A+  GC FP D LPIE+  LKTC +
Sbjct: 481 AAKSGGCYFPEDELPIEKVQLKTCFV 475

BLAST of MC06g1884 vs. NCBI nr
Match: XP_023532553.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 816 bits (2108), Expect = 2.46e-295
Identity = 402/505 (79.60%), Postives = 434/505 (85.94%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP KPT+  ++AA    VILLA VSL +VDG+KPCP S+  EFPAI+CRK++AVLT+F
Sbjct: 1   MDLPHKPTKTQITAARVSAVILLAFVSLGSVDGKKPCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNT+AFR+A+EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGVGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV +LPSYGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FH+NELNVTRPY+IEIMYSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHRNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC NTRIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCINTRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE+VTAINTQSAVR
Sbjct: 301 SGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+PEIKNINYRDVVA
Sbjct: 361 IKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALPEIKNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS VVPPPCAPLAK
Sbjct: 421 ENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSSDVVPPPCAPLAK 474

Query: 481 PA-EGVGCAFPTDRLPIEEFPLKTC 500
                 GC FP DRLPIE+  L TC
Sbjct: 481 EVVSSDGCPFPEDRLPIEDVKLMTC 474

BLAST of MC06g1884 vs. NCBI nr
Match: XP_022996001.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 808 bits (2088), Expect = 2.73e-292
Identity = 400/505 (79.21%), Postives = 432/505 (85.54%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSA----AVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP KPT+  ++A    AVILL LVSLA+VDG+KPCP S+  EF AI+CRK++AVLT+F
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNT+AFR+AIEHL  LA  GG+QL VP GKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV +LPSYGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHKNE NVTRPY+IEIMYSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC NTRIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCINTRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE+VTAINTQSAVR
Sbjct: 301 SGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+PEIKNINYRDVVA
Sbjct: 361 IKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALPEIKNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS VVP PCAPLAK
Sbjct: 421 ENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSSDVVPLPCAPLAK 474

Query: 481 PA-EGVGCAFPTDRLPIEEFPLKTC 500
                 GC FP D+LPIE+  L TC
Sbjct: 481 EVVSSDGCPFPEDQLPIEDVKLMTC 474

BLAST of MC06g1884 vs. NCBI nr
Match: XP_008437531.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK05998.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 808 bits (2087), Expect = 3.74e-292
Identity = 399/506 (78.85%), Postives = 435/506 (85.97%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP+KPT+ H +AA    VILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+F
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNT AFR+AIEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWP VAILPSYG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHK ELN+TRPY+IEI+YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC+N RIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCSNIRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE+VT INTQSAVR
Sbjct: 301 SGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTGINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+PEI NINYRDVVA
Sbjct: 361 IKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALPEITNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS VVPPPCAPLAK
Sbjct: 421 ENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISSDVVPPPCAPLAK 474

Query: 481 PAEGVGCAFPTDRLPIEEFPLKTCSI 502
            A+  GC FP D+LPIE+  LKTC +
Sbjct: 481 AAKSGGCDFPADQLPIEKVQLKTCFV 474

BLAST of MC06g1884 vs. NCBI nr
Match: XP_022957618.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 808 bits (2087), Expect = 3.88e-292
Identity = 398/505 (78.81%), Postives = 431/505 (85.35%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP KPT+  ++AA    VILLALVSL +VDG+K CP S+  EFPAI+CRK++AVLT+F
Sbjct: 1   MDLPHKPTKTQITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G +GDGVTSNT+AFR+A+EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGIGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV +LPSYGRGRDAP GRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHKNE NVTRPY+IEIMYS+QIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC NTRIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCINTRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE+VTAINTQSAVR
Sbjct: 301 SGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTA GRGG+VKDIFVR+MFLSTMKYVFWMTGDYKSHPD +FDP A+PEIKNINYRDVVA
Sbjct: 361 IKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGDYKSHPDEKFDPKALPEIKNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARL+GISGDPFTDIC++N+ IGLT K KKLQWNCTNVEG+SS VVPPPCAPLAK
Sbjct: 421 ENVTYSARLDGISGDPFTDICISNIKIGLTAKQKKLQWNCTNVEGYSSDVVPPPCAPLAK 474

Query: 481 PA-EGVGCAFPTDRLPIEEFPLKTC 500
                 GC FP DRLPIE+  L TC
Sbjct: 481 EVVSSDGCPFPEDRLPIEDLKLMTC 474

BLAST of MC06g1884 vs. ExPASy TrEMBL
Match: A0A6J1K3G8 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1)

HSP 1 Score: 808 bits (2088), Expect = 1.32e-292
Identity = 400/505 (79.21%), Postives = 432/505 (85.54%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSA----AVILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP KPT+  ++A    AVILL LVSLA+VDG+KPCP S+  EF AI+CRK++AVLT+F
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNT+AFR+AIEHL  LA  GG+QL VP GKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV +LPSYGRGRDAPGGRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHKNE NVTRPY+IEIMYSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC NTRIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCINTRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE+VTAINTQSAVR
Sbjct: 301 SGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTA GRGG+VKDIFVR+MFLSTMKYVFWMTG+YKSHPD +FDP A+PEIKNINYRDVVA
Sbjct: 361 IKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGNYKSHPDEKFDPKALPEIKNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARL+GISGDPFTDIC++NV IGLT KPKKLQWNCTNVEG+SS VVP PCAPLAK
Sbjct: 421 ENVTYSARLDGISGDPFTDICISNVKIGLTAKPKKLQWNCTNVEGYSSDVVPLPCAPLAK 474

Query: 481 PA-EGVGCAFPTDRLPIEEFPLKTC 500
                 GC FP D+LPIE+  L TC
Sbjct: 481 EVVSSDGCPFPEDQLPIEDVKLMTC 474

BLAST of MC06g1884 vs. ExPASy TrEMBL
Match: A0A5D3C461 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00950 PE=3 SV=1)

HSP 1 Score: 808 bits (2087), Expect = 1.81e-292
Identity = 399/506 (78.85%), Postives = 435/506 (85.97%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP+KPT+ H +AA    VILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+F
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNT AFR+AIEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWP VAILPSYG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHK ELN+TRPY+IEI+YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC+N RIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCSNIRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE+VT INTQSAVR
Sbjct: 301 SGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTGINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+PEI NINYRDVVA
Sbjct: 361 IKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALPEITNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS VVPPPCAPLAK
Sbjct: 421 ENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISSDVVPPPCAPLAK 474

Query: 481 PAEGVGCAFPTDRLPIEEFPLKTCSI 502
            A+  GC FP D+LPIE+  LKTC +
Sbjct: 481 AAKSGGCDFPADQLPIEKVQLKTCFV 474

BLAST of MC06g1884 vs. ExPASy TrEMBL
Match: A0A1S3AUV5 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1)

HSP 1 Score: 808 bits (2087), Expect = 1.81e-292
Identity = 399/506 (78.85%), Postives = 435/506 (85.97%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP+KPT+ H +AA    VILLALVSLATV+G++ CP +N  + PAINCR+++AVLT+F
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNT AFR+AIEHL PLAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWP VAILPSYG GRDAPGGR+SSLIYGTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHK ELN+TRPY+IEI+YSDQIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC+N RIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCSNIRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE L+IRRLTCISPDSAGIALGSEMSGGIR+VRIE+VT INTQSAVR
Sbjct: 301 SGWDQYGIKFGMPTEDLVIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTGINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+PEI NINYRDVVA
Sbjct: 361 IKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDEKFDPAALPEITNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARLEGISGDPFT+IC++NV IGLT KPKKLQWNCT+VEG SS VVPPPCAPLAK
Sbjct: 421 ENVTYSARLEGISGDPFTNICISNVKIGLTAKPKKLQWNCTDVEGISSDVVPPPCAPLAK 474

Query: 481 PAEGVGCAFPTDRLPIEEFPLKTCSI 502
            A+  GC FP D+LPIE+  LKTC +
Sbjct: 481 AAKSGGCDFPADQLPIEKVQLKTCFV 474

BLAST of MC06g1884 vs. ExPASy TrEMBL
Match: A0A6J1H0R5 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV=1)

HSP 1 Score: 808 bits (2087), Expect = 1.88e-292
Identity = 398/505 (78.81%), Postives = 431/505 (85.35%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           MDLP KPT+  ++AA    VILLALVSL +VDG+K CP S+  EFPAI+CRK++AVLT+F
Sbjct: 1   MDLPHKPTKTQITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G +GDGVTSNT+AFR+A+EHL  LA  GGAQL VP GKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGIGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180
           LASQDESEWPLV +LPSYGRGRDAP GRFSSLI+GTNLTDVVITGNNGTIDGQGSYWWDK
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPN 240
           FHKNE NVTRPY+IEIMYS+QIQISNLTLVNSPSWFVHPIYS NVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVK 300
           TDGIDP                               DSC NTRIEDCFIVSGDDCIAVK
Sbjct: 241 TDGIDP-------------------------------DSCINTRIEDCFIVSGDDCIAVK 300

Query: 301 SGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVR 360
           SGWDQYGI+FGMPTE LLIRRLTCISPDSAGIALGSEMSGGIR+VRIE+VTAINTQSAVR
Sbjct: 301 SGWDQYGIQFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVR 360

Query: 361 IKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVA 420
           IKTA GRGG+VKDIFVR+MFLSTMKYVFWMTGDYKSHPD +FDP A+PEIKNINYRDVVA
Sbjct: 361 IKTAIGRGGYVKDIFVRQMFLSTMKYVFWMTGDYKSHPDEKFDPKALPEIKNINYRDVVA 420

Query: 421 ENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPLAK 480
           ENVTY+ARL+GISGDPFTDIC++N+ IGLT K KKLQWNCTNVEG+SS VVPPPCAPLAK
Sbjct: 421 ENVTYSARLDGISGDPFTDICISNIKIGLTAKQKKLQWNCTNVEGYSSDVVPPPCAPLAK 474

Query: 481 PA-EGVGCAFPTDRLPIEEFPLKTC 500
                 GC FP DRLPIE+  L TC
Sbjct: 481 EVVSSDGCPFPEDRLPIEDLKLMTC 474

BLAST of MC06g1884 vs. ExPASy TrEMBL
Match: A0A0A0KNF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1)

HSP 1 Score: 780 bits (2015), Expect = 1.79e-281
Identity = 386/508 (75.98%), Postives = 430/508 (84.65%), Query Frame = 0

Query: 1   MDLPRKPTQAHVSAA----VILLALVSLATVDGRKPCPNSNCEEFPAINCRKYSAVLTEF 60
           M LP+ PT+ H +AA    VILLAL+SLATV+G++ CP +N  + PAI+CRK++A++T+F
Sbjct: 1   MYLPQDPTKTHDAAARISAVILLALLSLATVEGKRSCP-TNYLQVPAISCRKHTALITDF 60

Query: 61  GAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQI 120
           G VGDGVTSNT AFR+AIEHL  LAA GGAQL VP GKWLTGSFNLTSHFTLF+HKDA+I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKI 120

Query: 121 LASQ--DESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWW 180
           L SQ  +ESEWP VA+LPSYG GRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQG+YWW
Sbjct: 121 LGSQVENESEWPQVALLPSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWW 180

Query: 181 DKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDS 240
           DKFHK ELN+TRPYLIEIMYSDQIQIS+LTLVNSPSWFVHPIYS NVIIQGLTILAPIDS
Sbjct: 181 DKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDS 240

Query: 241 PNTDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIA 300
           PNTDGIDP                               DSC+NTRIEDCFIVSGDDCIA
Sbjct: 241 PNTDGIDP-------------------------------DSCSNTRIEDCFIVSGDDCIA 300

Query: 301 VKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSA 360
           VKSGWDQYGI+FGMPTE L+I+RLTCISPDSAG+ALGSEMSGGIR+VRIE+ T INTQSA
Sbjct: 301 VKSGWDQYGIKFGMPTEDLVIKRLTCISPDSAGVALGSEMSGGIRNVRIENFTGINTQSA 360

Query: 361 VRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDV 420
           VRIKTARGRGGFVKDIFVR M+LSTMKYVFWMTG+YKSHPD +FDPAA+PEI NINYRDV
Sbjct: 361 VRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMTGNYKSHPDDKFDPAALPEITNINYRDV 420

Query: 421 VAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPL 480
           VAENVTY+ARLEGISGDPFT+IC++NV IGLT  PKKLQWNCT+VEGFSS VVPPPCAPL
Sbjct: 421 VAENVTYSARLEGISGDPFTNICISNVKIGLTATPKKLQWNCTDVEGFSSDVVPPPCAPL 476

Query: 481 AKPAEGVGCAFPTDRLPIEEFPLKTCSI 502
           AK  +  GC FP D+LPIE   LKTC +
Sbjct: 481 AKAGKSGGCDFPEDQLPIERVQLKTCFV 476

BLAST of MC06g1884 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 632.1 bits (1629), Expect = 3.8e-181
Identity = 302/465 (64.95%), Postives = 358/465 (76.99%), Query Frame = 0

Query: 40  EFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS 99
           EF A+NCRK+SA+LT+FGAVGDG TSNT AFR AI  L  +A  GGAQL VP GKWLTGS
Sbjct: 34  EFSALNCRKHSAILTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTGS 93

Query: 100 FNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPG-GRFSSLIYGTNLTDVV 159
           FNLTSHFTLF+ + A ILASQDESEWP++A LPSYG+GRD  G GRF+SLI GTNLTDVV
Sbjct: 94  FNLTSHFTLFIQRGATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDVV 153

Query: 160 ITGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYS 219
           ITGNNGTI+GQG YWWDKF K +  +TRPYLIEI++S  IQISN+TL++SPSW +HP+Y 
Sbjct: 154 ITGNNGTINGQGQYWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVYC 213

Query: 220 SNVIIQGLTILAPIDSPNTDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTN 279
           ++VI++ +T+LAP+  PNTDGI+P                               DSCTN
Sbjct: 214 NSVIVKSVTVLAPVTVPNTDGINP-------------------------------DSCTN 273

Query: 280 TRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGI 339
           T IEDC+IVSGDDCIAVKSGWDQYGI+FGMPT+ L IRRLTCISP SAG+ALGSEMSGGI
Sbjct: 274 TLIEDCYIVSGDDCIAVKSGWDQYGIKFGMPTQQLSIRRLTCISPKSAGVALGSEMSGGI 333

Query: 340 RDVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEF 399
           +DVRIEDVT  NT+SA+RIKTA GRG +VKDI+ RR+ + TMKYVFWM+G+Y SHPD  F
Sbjct: 334 KDVRIEDVTLTNTESAIRIKTAVGRGAYVKDIYARRITMKTMKYVFWMSGNYGSHPDEGF 393

Query: 400 DPAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTN 459
           DP A+PEI NINYRD+ AENVT +A L+GI  DPFT IC++NVTI L  K KK+QWNCT+
Sbjct: 394 DPKALPEITNINYRDMTAENVTMSASLDGIDKDPFTGICISNVTIALAAKAKKMQWNCTD 453

Query: 460 VEGFSSGVVPPPCAPLAK---PAEGVGCAFPTDRLPIEEFPLKTC 501
           V G +S V P PC+ L +    A+ V CAFP+D +PIE   LK C
Sbjct: 454 VAGVTSRVTPEPCSLLPEKKAQAKNVDCAFPSDLIPIESVVLKKC 467

BLAST of MC06g1884 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 632.1 bits (1629), Expect = 3.8e-181
Identity = 304/463 (65.66%), Postives = 355/463 (76.67%), Query Frame = 0

Query: 40  EFPAINCRKYSAVLTEFGAVGDGVTSNTHAFRQAIEHLKPLAAAGGAQLFVPAGKWLTGS 99
           E+ AI+CR YSA L EFGAVGDGVTSNT AFR A+  L   A  GG+ LFVPAG+WLTG+
Sbjct: 57  EYSAISCRAYSASLDEFGAVGDGVTSNTAAFRDAVSQLSRFADYGGSLLFVPAGRWLTGN 116

Query: 100 FNLTSHFTLFLHKDAQILASQDESEWPLVAILPSYGRGRDAPGGRFSSLIYGTNLTDVVI 159
           FNLTSHFTLFLH+DA ILASQ+ES++ ++  LPSYGRGRD  GGRF SL++G+NLTDVVI
Sbjct: 117 FNLTSHFTLFLHRDAVILASQEESDYEVIEPLPSYGRGRDTDGGRFISLLFGSNLTDVVI 176

Query: 160 TGNNGTIDGQGSYWWDKFHKNELNVTRPYLIEIMYSDQIQISNLTLVNSPSWFVHPIYSS 219
           TG NGTIDGQG  WW KF + EL  TRPYLIEIM+SD IQISNLT +NSPSW +HP+YSS
Sbjct: 177 TGENGTIDGQGEPWWGKFKRGELKYTRPYLIEIMHSDGIQISNLTFLNSPSWHIHPVYSS 236

Query: 220 NVIIQGLTILAPIDSPNTDGIDPGSFNFQNYGLGLRFVFSSSSSILIPNFSIQFDSCTNT 279
           N+ IQGLTILAP+  PNTDGI+P                               DSCTNT
Sbjct: 237 NIYIQGLTILAPVTVPNTDGINP-------------------------------DSCTNT 296

Query: 280 RIEDCFIVSGDDCIAVKSGWDQYGIRFGMPTEHLLIRRLTCISPDSAGIALGSEMSGGIR 339
           RIEDC+IVSGDDCIAVKSGWDQYGI +GMPT+ LLIRRLTCISPDSA IALGSEMSGGI 
Sbjct: 297 RIEDCYIVSGDDCIAVKSGWDQYGINYGMPTKQLLIRRLTCISPDSAVIALGSEMSGGIE 356

Query: 340 DVRIEDVTAINTQSAVRIKTARGRGGFVKDIFVRRMFLSTMKYVFWMTGDYKSHPDPEFD 399
           DVR ED+ AIN++S +RIKTA GRGG+VKD++VR M + TMKYVFWMTG Y SHPD  +D
Sbjct: 357 DVRAEDIVAINSESGIRIKTAIGRGGYVKDVYVRGMTMMTMKYVFWMTGSYGSHPDDHYD 416

Query: 400 PAAIPEIKNINYRDVVAENVTYAARLEGISGDPFTDICVTNVTIGLTKKPKKLQWNCTNV 459
           P A+P I+NINY+D+VAENVT  A+L GISGD FT IC++NVTI L+KKPKK+ WNCT+V
Sbjct: 417 PKALPVIQNINYQDMVAENVTMPAQLAGISGDQFTGICISNVTITLSKKPKKVLWNCTDV 476

Query: 460 EGFSSGVVPPPC--APLAKPAEGVGCAFPTDRLPIEEFPLKTC 501
            G++SGV P PC   P  +P   V C FP D +PI+E  L+ C
Sbjct: 477 SGYTSGVTPQPCQLLPEKQPGTVVPCNFPEDPIPIDEVKLQRC 488

BLAST of MC06g1884 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 629.0 bits (1621), Expect = 3.3e-180
Identity = 306/500 (61.20%), Postives = 373/500 (74.60%), Query Frame = 0

Query: 12  VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 71
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 72  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 131
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 132 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 191
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 192 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPGSFNFQNY 251
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+P        
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINP-------- 244

Query: 252 GLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPT 311
                                  DSCTNTRIEDC+I+SGDDCIAVKSGWD+YGI FGMPT
Sbjct: 245 -----------------------DSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPT 304

Query: 312 EHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDI 371
           +HL+IRRLTCISP SA IALGSEMSGGI DVR ED+TA  T+S VRIKTA GRG FVK+I
Sbjct: 305 KHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNI 364

Query: 372 FVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISG 431
           +V+ M L TMK+VFWMTG+YK+H D  +DP A+PEI  INYRD+VAENV+ A RLEGISG
Sbjct: 365 YVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISG 424

Query: 432 DPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPL---------AKPAEGV 491
           DPFT IC++N TI +  K KK  W C++VEG +SGV P PC  L          K  +G 
Sbjct: 425 DPFTGICISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLLDGQESETTKKKMIDG- 472

Query: 492 GCAFPTDRLPIEEFPLKTCS 502
           GC FPTD L I+   LKTCS
Sbjct: 485 GCDFPTDVLEIDNVELKTCS 472

BLAST of MC06g1884 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 629.0 bits (1621), Expect = 3.3e-180
Identity = 306/500 (61.20%), Postives = 373/500 (74.60%), Query Frame = 0

Query: 12  VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 71
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 72  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 131
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 132 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 191
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 192 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPGSFNFQNY 251
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+P        
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINP-------- 244

Query: 252 GLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPT 311
                                  DSCTNTRIEDC+I+SGDDCIAVKSGWD+YGI FGMPT
Sbjct: 245 -----------------------DSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPT 304

Query: 312 EHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDI 371
           +HL+IRRLTCISP SA IALGSEMSGGI DVR ED+TA  T+S VRIKTA GRG FVK+I
Sbjct: 305 KHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNI 364

Query: 372 FVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISG 431
           +V+ M L TMK+VFWMTG+YK+H D  +DP A+PEI  INYRD+VAENV+ A RLEGISG
Sbjct: 365 YVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISG 424

Query: 432 DPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPL---------AKPAEGV 491
           DPFT IC++N TI +  K KK  W C++VEG +SGV P PC  L          K  +G 
Sbjct: 425 DPFTGICISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLLDGQESETTKKKMIDG- 472

Query: 492 GCAFPTDRLPIEEFPLKTCS 502
           GC FPTD L I+   LKTCS
Sbjct: 485 GCDFPTDVLEIDNVELKTCS 472

BLAST of MC06g1884 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 629.0 bits (1621), Expect = 3.3e-180
Identity = 306/500 (61.20%), Postives = 373/500 (74.60%), Query Frame = 0

Query: 12  VSAAVILLALVSLATV-DGRKPCPNSNCEEFPAINCRKYSAVLTEFGAVGDGVTSNTHAF 71
           ++  V ++ L +L T+  GR+   +    E+ AI CR +SA +TE+G VGDG T NT AF
Sbjct: 5   LACIVTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAF 64

Query: 72  RQAIEHLKPLAAAGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVAI 131
           + A++HL   ++ GGAQLFVPAGKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P++  
Sbjct: 65  QSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKA 124

Query: 132 LPSYGRGRDAPGGRFSSLIYGTNLTDVVITGNNGTIDGQGSYWWDKFHKNELNVTRPYLI 191
           LPSYGRGRDA GGRF+SLI+GTNL+DV+ITGNNGTIDGQGS+WW KFH  +L  TRPYLI
Sbjct: 125 LPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLI 184

Query: 192 EIMYSDQIQISNLTLVNSPSWFVHPIYSSNVIIQGLTILAPIDSPNTDGIDPGSFNFQNY 251
           E+M+SD IQISNLT ++SPSW +HP+YSSN+I++G+TI+AP+ SPNTDGI+P        
Sbjct: 185 ELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINP-------- 244

Query: 252 GLGLRFVFSSSSSILIPNFSIQFDSCTNTRIEDCFIVSGDDCIAVKSGWDQYGIRFGMPT 311
                                  DSCTNTRIEDC+I+SGDDCIAVKSGWD+YGI FGMPT
Sbjct: 245 -----------------------DSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPT 304

Query: 312 EHLLIRRLTCISPDSAGIALGSEMSGGIRDVRIEDVTAINTQSAVRIKTARGRGGFVKDI 371
           +HL+IRRLTCISP SA IALGSEMSGGI DVR ED+TA  T+S VRIKTA GRG FVK+I
Sbjct: 305 KHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNI 364

Query: 372 FVRRMFLSTMKYVFWMTGDYKSHPDPEFDPAAIPEIKNINYRDVVAENVTYAARLEGISG 431
           +V+ M L TMK+VFWMTG+YK+H D  +DP A+PEI  INYRD+VAENV+ A RLEGISG
Sbjct: 365 YVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISG 424

Query: 432 DPFTDICVTNVTIGLTKKPKKLQWNCTNVEGFSSGVVPPPCAPL---------AKPAEGV 491
           DPFT IC++N TI +  K KK  W C++VEG +SGV P PC  L          K  +G 
Sbjct: 425 DPFTGICISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLLDGQESETTKKKMIDG- 472

Query: 492 GCAFPTDRLPIEEFPLKTCS 502
           GC FPTD L I+   LKTCS
Sbjct: 485 GCDFPTDVLEIDNVELKTCS 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL37.6e-17460.57Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW073.1e-2626.96Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q7M1E72.6e-2528.02Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q8RY293.6e-2224.65Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
P276442.3e-2129.76Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038906749.13.53e-29779.45probable polygalacturonase [Benincasa hispida][more]
XP_023532553.12.46e-29579.60probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
XP_022996001.12.73e-29279.21probable polygalacturonase [Cucurbita maxima][more]
XP_008437531.13.74e-29278.85PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative poly... [more]
XP_022957618.13.88e-29278.81probable polygalacturonase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1K3G81.32e-29279.21probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1[more]
A0A5D3C4611.81e-29278.85Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3AUV51.81e-29278.85probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1[more]
A0A6J1H0R51.88e-29278.81probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV... [more]
A0A0A0KNF91.79e-28175.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48950.13.8e-18164.95Pectin lyase-like superfamily protein [more]
AT4G23500.13.8e-18165.66Pectin lyase-like superfamily protein [more]
AT3G61490.13.3e-18061.20Pectin lyase-like superfamily protein [more]
AT3G61490.23.3e-18061.20Pectin lyase-like superfamily protein [more]
AT3G61490.33.3e-18061.20Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 310..332
e-value: 2800.0
score: 4.0
coord: 218..244
e-value: 920.0
score: 7.5
coord: 338..359
e-value: 560.0
score: 9.0
coord: 276..297
e-value: 430.0
score: 9.7
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 147..244
e-value: 4.1E-17
score: 62.2
coord: 271..460
e-value: 5.6E-18
score: 65.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 35..464
e-value: 1.9E-111
score: 375.0
IPR024535Pectate lyase superfamily proteinPFAMPF12708Pectate_lyase_3coord: 54..108
e-value: 2.1E-6
score: 27.8
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 15..244
NoneNo IPR availablePANTHERPTHR31339:SF12ENDO-POLYGALACTURONASE-LIKE PROTEINcoord: 270..501
coord: 15..244
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 270..501
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 52..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1884.1MC06g1884.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity