MC06g1813 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1813
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionGDSL esterase/lipase
LocationMC06: 25412913 .. 25418612 (-)
RNA-Seq ExpressionMC06g1813
SyntenyMC06g1813
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTTCTAGATCTCACTTTATAACTATAATGTTAGTTGTTTCGGCCATGAACTTCTTGTCGTGTCTTTGTCTAACGACATTCGTGTTTGGAGATTCGCTCGTCGATGTTGGGAATAACGATTACATTTTCACTCTGTCGAGGGCTGATTCGCCCCCCTACGGCATCGATTTCAAGCCCTCCGGCGGCCAACCCACCGGAAGGTTCACCAACGGTCGGACTATTTCTGATATTATTGGTAATGTGGATTTCTTATTAAGCTTACCAGAAACACACCATTTTGAAATGTAATTTAGAGTTTAGAATAATTTTAGACAAAATTGGTGATTAACTTAGACCCATTTTGATAACATTTTATGTTTTTAGTTTTTGATTTTTTTGTTTTTGATTTTGGATTTTTAGTTTGTGTTTTTAAAAAACAAAAAAGGGTACGGTATGATAACTATTTTTGTTTTTTGTTTTTGAAAACCTAAAAACCAAACCGATCACCATTTTTCTATTTCTAAAAAGTTACAAAGTTTAAAGTATATATTATAGAGCGAAAAATCTATATAAAGTAGAGATGACAATAGTGTGGAGTAGAAGTGAGGATCCCCTCTTCCATCCTCTTCTTGCAAAAATTTCTAATTCTCATTTCCACCTTGCTTTCCTATTCCAAGAATCGAGGTCCGAGTTCAAGAATCGTCACGATGAAAATTTTCATGAGAAAAGTTATTGGTTTAATAATAAATTTGATTTTTTTTATAATTTAAAAGATAAAAAGTAATAAAGATATATAATTTATATCTATTTTATTCAAAAATTATTATTTACTGATATAAATAATTAGTTTAAAAGATATTTAATATTTTATTCATAATTTAATAAAACAAATATATATTAATTATTTAGAAAATGAAATCGGGACGAGACATAGTGGAAACTGTATTCTCTGTTCTTAGAACCCTACGAGAATTTTTGTCATCTCTAAATGTAGAGACATGAAAATAAGAGTTTAGAGAGATAATATAAATTTTGAATGAGAAACTTTAGCGAGAGTAAATTTGAAAGAGTAAAGTTAAAGGACAAAAATAAAAAATCTTAAGTTATAAGTAAAAAGGAAAATTGAATGAGACAAAATTTAGATGGAGAAAAATTGAAAGATATATTTGTTAGAGAAAAATATAGAAAGAGTAAGAAAAGTTATAAAAGGCAAAATTTGAAAATGAAAAACCTTAAAATATACATATATTATACTTTTAGAGAAAGAAAGATGAGAGTAGCATGTTAGAAGAAAGATGAGAGTAGCATGTTAGAAAAAAATAAAGAAATAGAAATATATAGAAATGTAAATTTCAGAGGGAGAGAAATAATTGTAAATATTTTCAAGATGAAAATTATAGAGGGAGAAGAATTTTGAGAGAGAAATTTTGGTAAGAATATTTTAAAGAAAAAAAGTTACAGCATTAGAAGATAAAAAAGAAATTATGAAAACAGAAATTTGTTTCTGAAAACTATTTTTTTCCCCTTGAAATTTACTAAAAATTTCAAAAACAATCTTAAAAAACAAGCACGTTTTTTAAAAATGAAAAATCAAAAACTAAAACCAAAAACCAGAAAATGAACGAACTACTTGTCAAACGGGTGCTTAAATGTAAGTTTGGTGTAACTATAACGATGATGATTCCGTGTATTTGTTATTTTTTTTTGACCAGCTTAATTACGTTAGATTTGCAACCATTCAACAAAAATGTGTATTGAACACAAAAAAGGGAGATCTTATGAACAAGGGAAAAAAAGTGAGCGAAAAATGATTAAAAAAAAAAAAAAGCAATCTTATCAATCTTTTTAAAAATCAGAAATAAAAAACAGAAAATGAGATTTTTTTTTTTAATAGTAAGAGAAACAAAAATAGAAGAAACTATAAATTGTTTTTCTTACCAATGTTGCCAATGTTCTTTAAATAGCATGCCATATATATGTCGAATTGATATATATATATCAAAGCAACTTTATATTATAGATTAAAGTATTTATAAAGTATTTGAATCAGATGAATATCTTGGTGCCAAGTCATTTCCTCCACCATTTTTGGCTCCAAATACAGAAGTAGACACAATGTATAGAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTTTGTTATTTGTAAGTCCACTTTTACATTACTATATATGTTCTTAAAATTAAATATTATAAATTAAAACATTTCCTCCAAAAAACATACATAAAAGATAAATCACGAGCCACATTGTACTAGATTTAGAACAAATTAATTACCATTTTAGTTTATATGACTTATGTTTTGCTTTTAATCAGGGCCTTAATGATTTTAAATGGTTTAAATCTCTCTAAACCTCATCATTTCGGTACTAGATGATAGTTGTTATGTCGCACATTTTATATATCAGATCAAAAAACCATGAACTTTTAAGAGGTACTCAATAAGGCGATGTTTTAGGAGATTTAACAAGATCATGTATAATGGTCAAATTCAAGCTTTTAAAACAATAGGGTCGGGACTAAAATAGTAATTTAACCTAAATTCAACATTTTCAATATATTCTCAATACTCTCGACACACATGGACTCGAATATTGGCATGAAATTCAACGAGCAAAAATGTATACTGAAGATGTTTTTTTTTTCCTAATGTCAATGTAAAAATTACTAATAATTTGTAGAAAAATACAAGCTTTTGAATAAATTGTTTATGTGGAACACAGTTGGGAAGGATTCCACTAAGGGAGCAAGTGAAGAATTTTGAGGAGAGTAGAAGGTACATGGTGAAGGTAAAGGGAGAGAATGAAACAATGGAGGTTTTGAAGAATGCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAACTACATCCAACCCTCCATTCCAATTTTCCAAACAAACAAGGCTTCCCCAAGTGATTACTTGGATCATATGATCTCCAACATAACAATACAGCTCAAGGTAAAACGATATAAAATGTCTTAACATTCATATACTTCATCAAGTTTATTCTTGCATTGTTTATTAATATAGCTAGAAAGAAGCAAATTATCATTTTAGTATGTTTCTCACCATTTAAAAAAAACACAACACATGAATTTATAGTCTTGAAAACAAAAACTACTATGTTTTTTTCAGTTTTCAAAACTTAGACTTGGATTTTGGAAACAAATTTTAAAATTAATCCAAGAAATAATAAAGAGCAGTTGGAGGAAAAAGAAACAGTCGATTCATATATATTTACCTTTTTGAAGAGTTGCTATTAAAAACGTGTTTTGGCCAATTGCCTCCACCACGAAAGCCCTAGGCCAAAAGGTTGCCCACCATATATATGTAATTAAAAAGAAGAATGCAAGGACACATGAGAGTTTAGTGTCAATTGTTGGACAATATTGCTTTTTGGTAGGACTCAAAGAGCTTGGCATGATTTTGGGCAAAGAAAATTTTGAAACAAGCACTTATTTCAACCCATGTTCCCTACTTTTAGTTTTTGTCCAAACCACATAGTGGGTCAACTTTAAATAGGGTGTATGACTCACGATTTTATATCAGTTTGTCCTATTCATTAGTTTTGAGTCCCTTTGATAACATTTACGTTAAAACAAGAAAAATGAAAAATGAATAGACAAACAAGAAACATAAACATAAACAATTTTATATTTACGTTTGGTAATATTAATAGTGATTTATGTCGTTTTTTAGTTTGGTAACATTTTAAAAATAAAGACACAGGAAAAAAAAACAAGAACATTACTCGACGGACCCTTTGAGATTGACTTGTGGATGCTTTTGTTGATGAAGCGACTCCACGGTGTTGGGGCAAGAAAGGTTGTAGTGGTCGGAGTTGGACCACTTGGATGTATTCCATTTGTCAGGACACTGCAATTTGCACCAAATGATGAATGCTCAGAAGAAGTGAATCAGCTGATTGAAGCCTACAATTTTAGGCTGAGTGAAGTGGTTGACCAATTGAATCAAGAGTTCAACCCCAAAACCATGTTCGTTTATGCCAATTCCTATGCTGTCTTCATGCAGATTATTGATAATTATCAACAATATGGTTAGATTTCTCAAACCCCATATTTCACATGGCTTTTCTTTCCATTTTGGGTTAAAATATTAGAGGGTTTTTCCTAAACTAAGAAAAATTTTAAATTACCCATTTTTGTCGCTAGGATATTTTTAAAATTTATACTATATGGTTGTGTTAAATCTTCTAAAATGTCTTGGCTTTTTTTCTAACCTGCCACATTTCTTTTGGAGGTGGTAGGATAAAGGTGAATAAGTTGACCTTTAGTATAAGGTATTTGTAAAATTTAAATTGATTTATTATTTTAGGTTAATTATATTAGATTTTGAATCGGATATAAAATACGAGCTGGTTGGTTTAGTAATGTTTTCATCTCATATTTCTTGTTTCTCTTTTCCAAGTTTTTAGAAATATAAGTCTTTAGTAGCTATTTTTTTCCTCATTTTTAACAAATGTTTCCAAAATTATGTAGAAATTCTCGAAAACAACAAAATCAAATTAATGGTTTCTTCTTCTCATTTTAAAATTTTCCCAAAACATAAATAAAAACATTATTTTAACACTTTGACTTATAAAATGTAAAATAAAGTTTTTTAATATTAATTTTCATCAGTTTTCTAATTTAAAATTTTAGAAAACAAAAAATAAAAAAAAAGGTTACCAAAATAAAAAGAAATAAGAAATAGTTATTATAGTTATATTTTTCCTTTGAAAAATAAAAAACAGTAACGTATACCAAACACACCTAAATTGTGTTTTTTGGTAAAGAAAAATAGTATCTAAGGAGCCATATGTTTTGCTGCACACATCTTCAAAAACAATACTTCTCAAAACTCGTCCCTTAATTTCTCTCTCAACTGTTAAAGATCCCAATAAGTGACCTAACAAAGGTTTATAATGCAGCTACTCCTTTTATGGCCAATTAGTTTTGTAATGGAACCTATGAATATTGATATGGTATCAGAGCCCAAAAAGCTCAAATGAGTATCCGGTCCAAAAATGGGATCCCGACTAAGATCGATGAGCCAAAAGAGTCACGATCTTAAAGGGTCATGTTAAGGATCTCACATTGATAAAAGAAGGGATTTTATAATAGACATGACCTACCCCTCTCATTTACCAATTGGTTTTGAGATTGAACCCATGAATATTGATATCAACCACGTCCAAAAAAGATATAAAATAAGAGAAATGTTAATATAGGATTTTTTAGACAATGGTTTAACCAAAGTATAGGTACCGCAATGAAAATTTTAAAATCACAGAGACCCAAGTCGAAATCTAGCTCAAACTATTCAAACTAAAATTTATAATTATAAAAAACGCCATTTAAGCATAATCAAATGCACCATATATATACACATTTCTTCACAAAATCTTTTATAACTTTGATTTTTGAGCAATATGTTAGGGTTCGTGGATGCAAAGCAACCATGTTGTGGTGGATACTTCCCTCCTTTCGTTTGCTACAAGGGCCAAAACCAAAATAGAAGCTCTTCTCTTTGTGATGATCGATCGAAATATGTGTTTTGGGATGCTTATCACCCAACAGAAGCTGCTAATATCATCATTGCCAAAGAGCTCCTTGATGGAGATGAAACCATTAGCTCTCCTATCAACATTCGTCAACTCTACGCCTACCGT

mRNA sequence

ATGGAGTTTTCTAGATCTCACTTTATAACTATAATGTTAGTTGTTTCGGCCATGAACTTCTTGTCGTGTCTTTGTCTAACGACATTCGTGTTTGGAGATTCGCTCGTCGATGTTGGGAATAACGATTACATTTTCACTCTGTCGAGGGCTGATTCGCCCCCCTACGGCATCGATTTCAAGCCCTCCGGCGGCCAACCCACCGGAAGGTTCACCAACGGTCGGACTATTTCTGATATTATTGATGAATATCTTGGTGCCAAGTCATTTCCTCCACCATTTTTGGCTCCAAATACAGAAGTAGACACAATGTATAGAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTTTGTTATTTTTGGGAAGGATTCCACTAAGGGAGCAAGTGAAGAATTTTGAGGAGAGTAGAAGGTACATGGTGAAGGTAAAGGGAGAGAATGAAACAATGGAGGTTTTGAAGAATGCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAACTACATCCAACCCTCCATTCCAATTTTCCAAACAAACAAGGCTTCCCCAAGTGATTACTTGGATCATATGATCTCCAACATAACAATACAGCTCAAGCGACTCCACGGTGTTGGGGCAAGAAAGGTTGTAGTGGTCGGAGTTGGACCACTTGGATGTATTCCATTTGTCAGGACACTGCAATTTGCACCAAATGATGAATGCTCAGAAGAAGTGAATCAGCTGATTGAAGCCTACAATTTTAGGCTGAGTGAAGTGGTTGACCAATTGAATCAAGAGTTCAACCCCAAAACCATGTTCGTTTATGCCAATTCCTATGCTGTCTTCATGCAGATTATTGATAATTATCAACAATATGGGTTCGTGGATGCAAAGCAACCATGTTGTGGTGGATACTTCCCTCCTTTCGTTTGCTACAAGGGCCAAAACCAAAATAGAAGCTCTTCTCTTTGTGATGATCGATCGAAATATGTGTTTTGGGATGCTTATCACCCAACAGAAGCTGCTAATATCATCATTGCCAAAGAGCTCCTTGATGGAGATGAAACCATTAGCTCTCCTATCAACATTCGTCAACTCTACGCCTACCGT

Coding sequence (CDS)

ATGGAGTTTTCTAGATCTCACTTTATAACTATAATGTTAGTTGTTTCGGCCATGAACTTCTTGTCGTGTCTTTGTCTAACGACATTCGTGTTTGGAGATTCGCTCGTCGATGTTGGGAATAACGATTACATTTTCACTCTGTCGAGGGCTGATTCGCCCCCCTACGGCATCGATTTCAAGCCCTCCGGCGGCCAACCCACCGGAAGGTTCACCAACGGTCGGACTATTTCTGATATTATTGATGAATATCTTGGTGCCAAGTCATTTCCTCCACCATTTTTGGCTCCAAATACAGAAGTAGACACAATGTATAGAGGAATAAACTATGCTTCTGGAGCTTCTGGCATCTTGGATGAAACTGGTTTGTTATTTTTGGGAAGGATTCCACTAAGGGAGCAAGTGAAGAATTTTGAGGAGAGTAGAAGGTACATGGTGAAGGTAAAGGGAGAGAATGAAACAATGGAGGTTTTGAAGAATGCAATTTTCTCTTTAACAGTTGGATCCAATGATATAATCAACTACATCCAACCCTCCATTCCAATTTTCCAAACAAACAAGGCTTCCCCAAGTGATTACTTGGATCATATGATCTCCAACATAACAATACAGCTCAAGCGACTCCACGGTGTTGGGGCAAGAAAGGTTGTAGTGGTCGGAGTTGGACCACTTGGATGTATTCCATTTGTCAGGACACTGCAATTTGCACCAAATGATGAATGCTCAGAAGAAGTGAATCAGCTGATTGAAGCCTACAATTTTAGGCTGAGTGAAGTGGTTGACCAATTGAATCAAGAGTTCAACCCCAAAACCATGTTCGTTTATGCCAATTCCTATGCTGTCTTCATGCAGATTATTGATAATTATCAACAATATGGGTTCGTGGATGCAAAGCAACCATGTTGTGGTGGATACTTCCCTCCTTTCGTTTGCTACAAGGGCCAAAACCAAAATAGAAGCTCTTCTCTTTGTGATGATCGATCGAAATATGTGTTTTGGGATGCTTATCACCCAACAGAAGCTGCTAATATCATCATTGCCAAAGAGCTCCTTGATGGAGATGAAACCATTAGCTCTCCTATCAACATTCGTCAACTCTACGCCTACCGT

Protein sequence

MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIPIFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQLYAYR
Homology
BLAST of MC06g1813 vs. ExPASy Swiss-Prot
Match: Q9FJ25 (GDSL esterase/lipase At5g41890 OS=Arabidopsis thaliana OX=3702 GN=At5g41890 PE=3 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 6.6e-135
Identity = 226/343 (65.89%), Postives = 276/343 (80.47%), Query Frame = 0

Query: 27  TTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGA 86
           T F+FGDSLVDVGNN+YIFTLS+ADS PYGIDF PS GQPTGRFTNGRTISDI+ E LGA
Sbjct: 25  TNFIFGDSLVDVGNNNYIFTLSKADSSPYGIDFAPSNGQPTGRFTNGRTISDIVGEALGA 84

Query: 87  KSFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVK 146
           KS PPP+L PNTE +T+  GINYASGA+GILD+TGLLF+GR+PLREQV NFE+SR YMV+
Sbjct: 85  KSPPPPYLEPNTEANTIRNGINYASGAAGILDDTGLLFIGRVPLREQVSNFEKSREYMVR 144

Query: 147 VKGENETMEVLKNAIFSLTVGSNDIINYIQPSIPIFQTNKASPSDYL-DHMISNITIQLK 206
           V GEN T E+LKNA+F++T+GSNDI+NYIQPSIP F  +K  P+D L D M+ ++T  LK
Sbjct: 145 VIGENGTKEMLKNAMFTITIGSNDILNYIQPSIPFFSQDKL-PTDVLQDSMVLHLTTHLK 204

Query: 207 RLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQE 266
           RLH +G RK VVVGVGPLGCIPF R L   P  +CSE+VNQ++  YN +L   +  LN E
Sbjct: 205 RLHQLGGRKFVVVGVGPLGCIPFARALNLIPAGKCSEQVNQVVRGYNMKLIHSLKTLNNE 264

Query: 267 FNPK---TMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGGYFPPFVCYKGQNQNRSSSL 326
              +   T FVYANSY +F++++ NYQ +G  +A +PCCGGYFPPF C+KG NQN S + 
Sbjct: 265 LRSEDYNTTFVYANSYDLFLKLVLNYQLFGLKNADKPCCGGYFPPFACFKGPNQNSSQAA 324

Query: 327 CDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
           C+DRSK+VFWDAYHPTEAAN+I+AK LLDGD+T+++P NIR L
Sbjct: 325 CEDRSKFVFWDAYHPTEAANLIVAKALLDGDQTVATPFNIRYL 366

BLAST of MC06g1813 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 3.7e-85
Identity = 172/366 (46.99%), Postives = 227/366 (62.02%), Query Frame = 0

Query: 13  LVVSAMNFLSCLCL------TTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQP 72
           ++V ++ F S +CL        FVFGDSLVD GNN+Y+ TLS+A+  P GIDF    G P
Sbjct: 10  IIVLSVLFFSEVCLAGKKIPANFVFGDSLVDAGNNNYLATLSKANYVPNGIDF----GSP 69

Query: 73  TGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLG 132
           TGRFTNGRTI DI+ + LG+    PP+LAP T    +  G+NYASG SGIL+ TG LF  
Sbjct: 70  TGRFTNGRTIVDIVYQALGSDELTPPYLAPTTSGSLILNGVNYASGGSGILNSTGKLFGE 129

Query: 133 RIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDII-NYIQPSIPIFQTN 192
           RI +  Q+ NF  +R+ ++   GE+E  ++ ++AIFS+T GSND+I NY  P I   Q  
Sbjct: 130 RINVDAQLDNFATTRQDIISWIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVISTLQRK 189

Query: 193 KASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVN 252
             +P  ++D MIS   +QL RL+ +GARK+VV+ +GP+GCIPF R    A  + C  E N
Sbjct: 190 VVAPEVFVDTMISKFRLQLTRLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAEPN 249

Query: 253 QLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCC---- 312
           ++ + YN +L  +V++LN+     + FVY + + +   II NY  YGF   K PCC    
Sbjct: 250 EVAQMYNLKLKTLVEELNKNLQ-GSRFVYGDVFRIVDDIIQNYSSYGFESEKIPCCSLVG 309

Query: 313 --GGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSP 366
             GG  P   C         S +C DRSKYVFWD YHPTEAANIIIA+ LL GD +   P
Sbjct: 310 KVGGLIP---C------GPPSKVCMDRSKYVFWDPYHPTEAANIIIARRLLSGDTSDIYP 361

BLAST of MC06g1813 vs. ExPASy Swiss-Prot
Match: O80470 (GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.4e-84
Identity = 154/343 (44.90%), Postives = 226/343 (65.89%), Query Frame = 0

Query: 28  TFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAK 87
           +F+FGDSLVD GNN+Y+ TLSRA+  P GIDFK SGG PTGRFTNGRTI DI+ E LG+ 
Sbjct: 49  SFIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVGEELGSA 108

Query: 88  SFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKV 147
           ++  PFLAP+ +   +  G+NYASG  GI++ TG +F+ R+ +  QV  F  +R+    +
Sbjct: 109 NYAIPFLAPDAKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNTTRKQFDDL 168

Query: 148 KGENETME-VLKNAIFSLTVGSNDII-NYIQPSIPIFQTNKASPSDYLDHMISNITIQLK 207
            G+ +  + + K +IFS+T+G+ND + NY+ P + +      +P D++  M+ ++  QL 
Sbjct: 169 LGKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDMLEHLRDQLT 228

Query: 208 RLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQE 267
           RL+ + ARK V+  VGP+GCIP+ +T+     +EC +  N+L   YN RL  ++++LN++
Sbjct: 229 RLYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQYNVRLKSLLEELNKK 288

Query: 268 FNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGG---YFPPFVCYKGQNQNRSSSL 327
             P  MFV+AN Y + M++I NY +YGF  A + CCG    Y     C        +SSL
Sbjct: 289 L-PGAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGIIPC------GPTSSL 348

Query: 328 CDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
           C++R KYVFWD YHP+EAAN+IIAK+LL GD  + SP+N+ +L
Sbjct: 349 CEERDKYVFWDPYHPSEAANVIIAKQLLYGDVKVISPVNLSKL 384

BLAST of MC06g1813 vs. ExPASy Swiss-Prot
Match: Q9M2R9 (GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.4e-81
Identity = 157/346 (45.38%), Postives = 222/346 (64.16%), Query Frame = 0

Query: 28  TFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAK 87
           +FVFGDSLVD GNN+Y+ TLSRA+SPP GIDFKPS G PTGRFTNGRTI+DI+ E LG +
Sbjct: 35  SFVFGDSLVDAGNNNYLQTLSRANSPPNGIDFKPSRGNPTGRFTNGRTIADIVGEKLGQQ 94

Query: 88  SFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKV 147
           S+  P+LAPN   + +  G+NYASG  GIL+ TG +F+ R+ +  QV  F  +R+   K+
Sbjct: 95  SYAVPYLAPNASGEALLNGVNYASGGGGILNATGSVFVNRLGMDIQVDYFTNTRKQFDKL 154

Query: 148 KGENETME-VLKNAIFSLTVGSNDII-NYIQPSIPIFQTNKASPSDYLDHMISNITIQLK 207
            G+++  + + K ++FS+ +GSND + NY+ P +        +P  ++D MIS++  QLK
Sbjct: 155 LGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQARLTQTPETFVDDMISHLRNQLK 214

Query: 208 RLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQE 267
           RL+ + ARK VV  V P+GCIP+ +++    + +C +  N+L   YN RL +++    ++
Sbjct: 215 RLYDMDARKFVVGNVAPIGCIPYQKSINQLNDKQCVDLANKLAIQYNARLKDLLTVELKD 274

Query: 268 FNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCC------GGYFPPFVCYKGQNQNRS 327
                 FVYAN Y +FM +I N++ YGF  A + CC       G  P   C        +
Sbjct: 275 SLKDAHFVYANVYDLFMDLIVNFKDYGFRTASEACCETRGRLAGILP---C------GPT 334

Query: 328 SSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
           SSLC DRSK+VFWDAYHPTEAAN++IA +LL GD    +P N+  L
Sbjct: 335 SSLCTDRSKHVFWDAYHPTEAANLLIADKLLYGDSKFVTPFNLLHL 371

BLAST of MC06g1813 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.4e-63
Identity = 138/345 (40.00%), Postives = 202/345 (58.55%), Query Frame = 0

Query: 29  FVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAKS 88
           FVFGDSLVD GNN+++ +L+R++  PYGIDF  +G QPTGRF+NG+TI D I E LG   
Sbjct: 51  FVFGDSLVDNGNNNHLNSLARSNYLPYGIDF--AGNQPTGRFSNGKTIVDFIGELLGLPE 110

Query: 89  FPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKVK 148
            P      +  VD ++ G+NYAS A GIL+ETG     R  +  QV+NFE++   + +  
Sbjct: 111 IPAFMDTVDGGVDILH-GVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISRSM 170

Query: 149 GENETMEVLKNAIFSLTVGSNDII-NYIQPSIPIFQTNKASPSDYLDHMISNITIQLKRL 208
            +    E +  ++  +++G+ND I NY++P +    ++   P+ + D ++SN T  L  L
Sbjct: 171 RKESVKEYMAKSLVVVSLGNNDYINNYLKPRL-FLSSSIYDPTSFADLLLSNFTTHLLEL 230

Query: 209 HGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQEFN 268
           +G G RK V+ GVGPLGCIP     Q A   EC E VN++ E +N RL  +VD+LN +  
Sbjct: 231 YGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLNSDNK 290

Query: 269 --PKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGGYFPPFVCYKGQNQNRSSSL--- 328
              + +FVY N+Y   + I+ N   YGF    + CCG          G+N+   + L   
Sbjct: 291 TASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCG---------VGRNRGEITCLPLA 350

Query: 329 --CDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
             C  R ++VFWDA+HPT+A N+IIA    +G ++   PIN+ QL
Sbjct: 351 VPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQL 382

BLAST of MC06g1813 vs. NCBI nr
Match: XP_022158401.1 (GDSL esterase/lipase At5g41890 [Momordica charantia])

HSP 1 Score: 749 bits (1933), Expect = 7.00e-273
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60
           MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK
Sbjct: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60

Query: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120
           PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET
Sbjct: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120

Query: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180
           GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP
Sbjct: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180

Query: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240
           IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC
Sbjct: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240

Query: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300
           SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC
Sbjct: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300

Query: 301 CGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPI 360
           CGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPI
Sbjct: 301 CGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPI 360

Query: 361 NIRQLYAYR 369
           NIRQLYAYR
Sbjct: 361 NIRQLYAYR 369

BLAST of MC06g1813 vs. NCBI nr
Match: XP_038907274.1 (GDSL esterase/lipase At5g41890 [Benincasa hispida])

HSP 1 Score: 617 bits (1591), Expect = 5.95e-220
Identity = 303/349 (86.82%), Postives = 321/349 (91.98%), Query Frame = 0

Query: 25  CLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYL 84
           C TTFVFGDSLVDVGNNDYIFTLS+ADSPPYGIDFKPSGGQPTGRFTNGRTISDII EYL
Sbjct: 72  CFTTFVFGDSLVDVGNNDYIFTLSKADSPPYGIDFKPSGGQPTGRFTNGRTISDIIGEYL 131

Query: 85  GAKSFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYM 144
           GAKSFP PFLAP+ E DT+Y GINYASGASGILDETGLLFLGRIPLREQVKNFE SR YM
Sbjct: 132 GAKSFPLPFLAPSKEADTIYNGINYASGASGILDETGLLFLGRIPLREQVKNFEASRDYM 191

Query: 145 VKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIPIFQTNKASPSDYLDHMISNITIQL 204
           VKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP FQ NKASPSDYLDHM+SN+TI L
Sbjct: 192 VKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIPFFQRNKASPSDYLDHMVSNLTIHL 251

Query: 205 KRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQ 264
           K+LH +GARK VVVGVGPLGCIPFVR + F  N++CSEEVN+LIEAYNF+L+EVVDQLN 
Sbjct: 252 KQLHKLGARKFVVVGVGPLGCIPFVRAVHFVTNEKCSEEVNRLIEAYNFKLNEVVDQLNL 311

Query: 265 EFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGGYFPPFVCYKGQNQ--NRSSS- 324
           EF P TMFVYANSY VF +II NYQQYGFV+AKQPCCGGYFPPF+CYK QNQ  N+SSS 
Sbjct: 312 EFGPNTMFVYANSYTVFTKIIVNYQQYGFVNAKQPCCGGYFPPFICYKDQNQDQNQSSSS 371

Query: 325 -LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQLYAYR 369
            LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL+AYR
Sbjct: 372 FLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQLHAYR 420

BLAST of MC06g1813 vs. NCBI nr
Match: XP_022977773.1 (GDSL esterase/lipase At5g41890 [Cucurbita maxima])

HSP 1 Score: 610 bits (1574), Expect = 4.11e-218
Identity = 301/374 (80.48%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60
           M+FS+  +I +M VV ++NF   LC TTFVFGDSLVD GNNDYIFTLS+ADSPPYGIDFK
Sbjct: 1   MKFSKYSYI-MMFVVFSINFFPGLCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 60

Query: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120
           PSGG+PTGRFTNGRTISDII EYLGA+SFPPPFLAPNTE DT+Y GINYASGA+GILDET
Sbjct: 61  PSGGRPTGRFTNGRTISDIIGEYLGAESFPPPFLAPNTEADTIYTGINYASGAAGILDET 120

Query: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180
           G LFLGRIPLREQVK+FEESR Y+VKVKGENETM++LK AIFSLT GSNDIINY QPSIP
Sbjct: 121 GFLFLGRIPLREQVKHFEESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPSIP 180

Query: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240
            FQ+NK +PSDYLDHMISN+TI LK LH +GARK VVVGVGPLGCIPFVR +QF   ++C
Sbjct: 181 FFQSNKVAPSDYLDHMISNLTIHLKELHDLGARKFVVVGVGPLGCIPFVRAVQFVRGEKC 240

Query: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300
           SEEVN++IEAYN RLS +V +LNQEF PKTMFVYANSYAVFM+II NYQQYGFV+AKQPC
Sbjct: 241 SEEVNEVIEAYNVRLSGLVGELNQEFGPKTMFVYANSYAVFMKIIVNYQQYGFVNAKQPC 300

Query: 301 CGGYFPPFVCY---KGQNQNRSSS--LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360
           CG YFPPF+CY   + Q+QNRSSS  LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET
Sbjct: 301 CGAYFPPFICYNKGQSQDQNRSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360

Query: 361 ISSPINIRQLYAYR 369
           IS+PINIRQL+AYR
Sbjct: 361 ISAPINIRQLHAYR 373

BLAST of MC06g1813 vs. NCBI nr
Match: XP_004143942.3 (GDSL esterase/lipase At5g41890 [Cucumis sativus])

HSP 1 Score: 606 bits (1562), Expect = 2.97e-216
Identity = 302/372 (81.18%), Postives = 329/372 (88.44%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFL-SCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDF 60
           MEFS+S+   +M++V  +NF     C TTFVFGDSLVD GNNDYIFTLS+ADSPPYGIDF
Sbjct: 6   MEFSKSY--VVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDF 65

Query: 61  KPSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAP-NTEVDTM-YRGINYASGASGIL 120
           KPSGGQPTGRFTNGRTISDII EYLGAKSFPPPFLAP +T+ DT+ Y+GINYASGASGIL
Sbjct: 66  KPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGIL 125

Query: 121 DETGLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQP 180
           DETGLLFLGRI LREQVKNFEESR  MVKVKGENETMEVLKN+IFSLTVGSNDIINYIQP
Sbjct: 126 DETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQP 185

Query: 181 SIPIFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPN 240
           SIP  QTNK SPSDYLDHMISN+T+ LKRLH +GARK VVVGVGPLGCIPFVR + F  N
Sbjct: 186 SIPFLQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTN 245

Query: 241 DECSEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAK 300
           ++C EEVNQLIE YNFRL+  VDQLN EF   TMF+YANSYAVF +II NY+QYGFV+AK
Sbjct: 246 EKCLEEVNQLIETYNFRLNGAVDQLNLEFGLSTMFIYANSYAVFTKIIVNYRQYGFVNAK 305

Query: 301 QPCCGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETIS 360
           QPCC GYFPPF+CYK QNQ+ SS LC+DRSKYVFWDAYHPTEAANIIIAKELLDGDETI+
Sbjct: 306 QPCCVGYFPPFICYKDQNQSSSSFLCEDRSKYVFWDAYHPTEAANIIIAKELLDGDETIT 365

Query: 361 SPINIRQLYAYR 369
           SPINIRQLYAYR
Sbjct: 366 SPINIRQLYAYR 375

BLAST of MC06g1813 vs. NCBI nr
Match: XP_023545021.1 (GDSL esterase/lipase At5g41890 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 600 bits (1548), Expect = 3.74e-214
Identity = 298/374 (79.68%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60
           M FS+  +I +M VV ++NF   LC TTFVFGDSLVD GNNDYIFTLS+ADSPPYGIDFK
Sbjct: 1   MTFSKYSYI-MMFVVFSINFFPGLCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 60

Query: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120
           PSGGQPTGRFTNGRTISDII EYLGA+SFPPPFLAPNTE DT+Y GINYASGA+GILDET
Sbjct: 61  PSGGQPTGRFTNGRTISDIIGEYLGAESFPPPFLAPNTESDTIYTGINYASGAAGILDET 120

Query: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180
           G LFLGRIPLREQVK+F+ESR Y+VKVKGENETM++LK AIFSLT GSNDIINY Q SIP
Sbjct: 121 GFLFLGRIPLREQVKHFKESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQSSIP 180

Query: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240
            F +NK +PSDYLDHMISN+TI LK LH +GARK VVVGVGPLGCIPFVR +Q    ++C
Sbjct: 181 FFGSNKVAPSDYLDHMISNLTIHLKELHELGARKFVVVGVGPLGCIPFVRAVQLVRGEKC 240

Query: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300
           SEEVN++IEAYN RLS +V +LNQEF PKTMFVYANSYAVFM+II NYQQYGFV+AKQPC
Sbjct: 241 SEEVNEVIEAYNVRLSGLVGELNQEFGPKTMFVYANSYAVFMKIIVNYQQYGFVNAKQPC 300

Query: 301 CGGYFPPFVCY---KGQNQNRSSS--LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360
           CG YFPPF+CY   + Q+QNRSSS  LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET
Sbjct: 301 CGAYFPPFICYNKGQSQDQNRSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360

Query: 361 ISSPINIRQLYAYR 369
           IS+PINIRQL+AYR
Sbjct: 361 ISAPINIRQLHAYR 373

BLAST of MC06g1813 vs. ExPASy TrEMBL
Match: A0A6J1E0T3 (GDSL esterase/lipase At5g41890 OS=Momordica charantia OX=3673 GN=LOC111024890 PE=3 SV=1)

HSP 1 Score: 749 bits (1933), Expect = 3.39e-273
Identity = 369/369 (100.00%), Postives = 369/369 (100.00%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60
           MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK
Sbjct: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60

Query: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120
           PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET
Sbjct: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120

Query: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180
           GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP
Sbjct: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180

Query: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240
           IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC
Sbjct: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240

Query: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300
           SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC
Sbjct: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300

Query: 301 CGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPI 360
           CGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPI
Sbjct: 301 CGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPI 360

Query: 361 NIRQLYAYR 369
           NIRQLYAYR
Sbjct: 361 NIRQLYAYR 369

BLAST of MC06g1813 vs. ExPASy TrEMBL
Match: A0A6J1IR23 (GDSL esterase/lipase At5g41890 OS=Cucurbita maxima OX=3661 GN=LOC111477974 PE=3 SV=1)

HSP 1 Score: 610 bits (1574), Expect = 1.99e-218
Identity = 301/374 (80.48%), Postives = 334/374 (89.30%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60
           M+FS+  +I +M VV ++NF   LC TTFVFGDSLVD GNNDYIFTLS+ADSPPYGIDFK
Sbjct: 1   MKFSKYSYI-MMFVVFSINFFPGLCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 60

Query: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120
           PSGG+PTGRFTNGRTISDII EYLGA+SFPPPFLAPNTE DT+Y GINYASGA+GILDET
Sbjct: 61  PSGGRPTGRFTNGRTISDIIGEYLGAESFPPPFLAPNTEADTIYTGINYASGAAGILDET 120

Query: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180
           G LFLGRIPLREQVK+FEESR Y+VKVKGENETM++LK AIFSLT GSNDIINY QPSIP
Sbjct: 121 GFLFLGRIPLREQVKHFEESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPSIP 180

Query: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240
            FQ+NK +PSDYLDHMISN+TI LK LH +GARK VVVGVGPLGCIPFVR +QF   ++C
Sbjct: 181 FFQSNKVAPSDYLDHMISNLTIHLKELHDLGARKFVVVGVGPLGCIPFVRAVQFVRGEKC 240

Query: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300
           SEEVN++IEAYN RLS +V +LNQEF PKTMFVYANSYAVFM+II NYQQYGFV+AKQPC
Sbjct: 241 SEEVNEVIEAYNVRLSGLVGELNQEFGPKTMFVYANSYAVFMKIIVNYQQYGFVNAKQPC 300

Query: 301 CGGYFPPFVCY---KGQNQNRSSS--LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360
           CG YFPPF+CY   + Q+QNRSSS  LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET
Sbjct: 301 CGAYFPPFICYNKGQSQDQNRSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360

Query: 361 ISSPINIRQLYAYR 369
           IS+PINIRQL+AYR
Sbjct: 361 ISAPINIRQLHAYR 373

BLAST of MC06g1813 vs. ExPASy TrEMBL
Match: A0A0A0KM97 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148500 PE=3 SV=1)

HSP 1 Score: 606 bits (1562), Expect = 1.20e-216
Identity = 302/372 (81.18%), Postives = 329/372 (88.44%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFL-SCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDF 60
           MEFS+S+   +M++V  +NF     C TTFVFGDSLVD GNNDYIFTLS+ADSPPYGIDF
Sbjct: 1   MEFSKSY--VVMVIVFFINFFHGGFCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDF 60

Query: 61  KPSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAP-NTEVDTM-YRGINYASGASGIL 120
           KPSGGQPTGRFTNGRTISDII EYLGAKSFPPPFLAP +T+ DT+ Y+GINYASGASGIL
Sbjct: 61  KPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIYKGINYASGASGIL 120

Query: 121 DETGLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQP 180
           DETGLLFLGRI LREQVKNFEESR  MVKVKGENETMEVLKN+IFSLTVGSNDIINYIQP
Sbjct: 121 DETGLLFLGRISLREQVKNFEESRNAMVKVKGENETMEVLKNSIFSLTVGSNDIINYIQP 180

Query: 181 SIPIFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPN 240
           SIP  QTNK SPSDYLDHMISN+T+ LKRLH +GARK VVVGVGPLGCIPFVR + F  N
Sbjct: 181 SIPFLQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTN 240

Query: 241 DECSEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAK 300
           ++C EEVNQLIE YNFRL+  VDQLN EF   TMF+YANSYAVF +II NY+QYGFV+AK
Sbjct: 241 EKCLEEVNQLIETYNFRLNGAVDQLNLEFGLSTMFIYANSYAVFTKIIVNYRQYGFVNAK 300

Query: 301 QPCCGGYFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETIS 360
           QPCC GYFPPF+CYK QNQ+ SS LC+DRSKYVFWDAYHPTEAANIIIAKELLDGDETI+
Sbjct: 301 QPCCVGYFPPFICYKDQNQSSSSFLCEDRSKYVFWDAYHPTEAANIIIAKELLDGDETIT 360

Query: 361 SPINIRQLYAYR 369
           SPINIRQLYAYR
Sbjct: 361 SPINIRQLYAYR 370

BLAST of MC06g1813 vs. ExPASy TrEMBL
Match: A0A1S3AUU3 (GDSL esterase/lipase At5g41890 OS=Cucumis melo OX=3656 GN=LOC103483079 PE=3 SV=1)

HSP 1 Score: 599 bits (1545), Expect = 4.82e-214
Identity = 301/373 (80.70%), Postives = 332/373 (89.01%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFLSC-LCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDF 60
           M FS+S+   +M++V ++NF    LC TTFVFGDSLVD GNNDYIFTLS+A+SPPYGIDF
Sbjct: 1   MVFSKSY--VVMVIVFSINFFDGGLCFTTFVFGDSLVDAGNNDYIFTLSKANSPPYGIDF 60

Query: 61  KPSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAP-NTEVDTM-YRGINYASGASGIL 120
           KPSGGQPTGRFTNGRTISDII EYLGAKSFPPPFLAP +T+ DT+ ++GINYASGASGIL
Sbjct: 61  KPSGGQPTGRFTNGRTISDIIGEYLGAKSFPPPFLAPISTQSDTIIHKGINYASGASGIL 120

Query: 121 DETGLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQP 180
           DETGLLFLGRI LREQVKNFEESR  MVK+KGENETMEVLKN+IFSLTVGSNDIINYIQP
Sbjct: 121 DETGLLFLGRISLREQVKNFEESRNEMVKLKGENETMEVLKNSIFSLTVGSNDIINYIQP 180

Query: 181 SIPIFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPN 240
           SIP FQTNK SPSDYLDHMISN+T+ LKRLH +GARK VVVGVGPLGCIPFVR + F  N
Sbjct: 181 SIPFFQTNKPSPSDYLDHMISNLTVHLKRLHALGARKFVVVGVGPLGCIPFVRAIHFVTN 240

Query: 241 DECSEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAK 300
           ++C EEVN+LIE YNF+LS VVDQLN EF   TMFVYANSYAVF +II NY+QYGFV+AK
Sbjct: 241 EKCLEEVNRLIETYNFKLSGVVDQLNLEFGLNTMFVYANSYAVFTKIIVNYRQYGFVNAK 300

Query: 301 QPCCGGYFPPFVCYKG-QNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETI 360
           QPCC GYFPPF+CYK  QNQ+ +S LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETI
Sbjct: 301 QPCCVGYFPPFICYKDRQNQSSTSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETI 360

Query: 361 SSPINIRQLYAYR 369
           +SPINIRQLYAYR
Sbjct: 361 TSPINIRQLYAYR 371

BLAST of MC06g1813 vs. ExPASy TrEMBL
Match: A0A6J1GD95 (GDSL esterase/lipase At5g41890 OS=Cucurbita moschata OX=3662 GN=LOC111453124 PE=3 SV=1)

HSP 1 Score: 599 bits (1545), Expect = 5.19e-214
Identity = 297/374 (79.41%), Postives = 331/374 (88.50%), Query Frame = 0

Query: 1   MEFSRSHFITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFK 60
           M  S+  +ITI +V S +NF   LC TTFVFGDSLVD GNNDYIFTLS+ADSPPYGIDFK
Sbjct: 1   MTVSKYSYITIFVVFS-INFFPGLCFTTFVFGDSLVDAGNNDYIFTLSKADSPPYGIDFK 60

Query: 61  PSGGQPTGRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDET 120
           PSGGQPTGRFTNGRTISDII EYLGA+SFPPPFLAPNTE DT++ GINYASGA+GILDET
Sbjct: 61  PSGGQPTGRFTNGRTISDIIGEYLGAESFPPPFLAPNTESDTIFTGINYASGAAGILDET 120

Query: 121 GLLFLGRIPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIP 180
           G LFLGRIPLREQVK+F+ESR Y+VKVKGENETM++LK AIFSLT GSNDIINY QPSIP
Sbjct: 121 GFLFLGRIPLREQVKHFKESRDYIVKVKGENETMKLLKEAIFSLTFGSNDIINYFQPSIP 180

Query: 181 IFQTNKASPSDYLDHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDEC 240
            F +NK +PSDYLD+MISN+TI LK LH +GARK VVVGVGPLGCIPFVR +QF   ++C
Sbjct: 181 FFGSNKVAPSDYLDYMISNLTIHLKELHELGARKFVVVGVGPLGCIPFVRAVQFVRGEKC 240

Query: 241 SEEVNQLIEAYNFRLSEVVDQLNQEFNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPC 300
           SEEVN++IEAYN RLS +V +LNQEF PKTMFVYANSYAVFM+II NY+QYGFV+AKQPC
Sbjct: 241 SEEVNEVIEAYNVRLSGLVGELNQEFGPKTMFVYANSYAVFMKIIVNYRQYGFVNAKQPC 300

Query: 301 CGGYFPPFVCY---KGQNQNRSSS--LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360
           CG YFPPF+CY   + Q+QNRSSS  LCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET
Sbjct: 301 CGAYFPPFICYNKGQSQDQNRSSSSFLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDET 360

Query: 361 ISSPINIRQLYAYR 369
           IS+PINIRQL+AYR
Sbjct: 361 ISTPINIRQLHAYR 373

BLAST of MC06g1813 vs. TAIR 10
Match: AT5G41890.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.0e-135
Identity = 229/362 (63.26%), Postives = 283/362 (78.18%), Query Frame = 0

Query: 8   FITIMLVVSAMNFLSCLCLTTFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPT 67
           F    L++   +F +    T F+FGDSLVDVGNN+YIFTLS+ADS PYGIDF PS GQPT
Sbjct: 12  FNCTFLLLWLSHFQAAQSFTNFIFGDSLVDVGNNNYIFTLSKADSSPYGIDFAPSNGQPT 71

Query: 68  GRFTNGRTISDIIDEYLGAKSFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGR 127
           GRFTNGRTISDI+ E LGAKS PPP+L PNTE +T+  GINYASGA+GILD+TGLLF+GR
Sbjct: 72  GRFTNGRTISDIVGEALGAKSPPPPYLEPNTEANTIRNGINYASGAAGILDDTGLLFIGR 131

Query: 128 IPLREQVKNFEESRRYMVKVKGENETMEVLKNAIFSLTVGSNDIINYIQPSIPIFQTNKA 187
           +PLREQV NFE+SR YMV+V GEN T E+LKNA+F++T+GSNDI+NYIQPSIP F  +K 
Sbjct: 132 VPLREQVSNFEKSREYMVRVIGENGTKEMLKNAMFTITIGSNDILNYIQPSIPFFSQDKL 191

Query: 188 SPSDYL-DHMISNITIQLKRLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQ 247
            P+D L D M+ ++T  LKRLH +G RK VVVGVGPLGCIPF R L   P  +CSE+VNQ
Sbjct: 192 -PTDVLQDSMVLHLTTHLKRLHQLGGRKFVVVGVGPLGCIPFARALNLIPAGKCSEQVNQ 251

Query: 248 LIEAYNFRLSEVVDQLNQEFNPK---TMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGG 307
           ++  YN +L   +  LN E   +   T FVYANSY +F++++ NYQ +G  +A +PCCGG
Sbjct: 252 VVRGYNMKLIHSLKTLNNELRSEDYNTTFVYANSYDLFLKLVLNYQLFGLKNADKPCCGG 311

Query: 308 YFPPFVCYKGQNQNRSSSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIR 366
           YFPPF C+KG NQN S + C+DRSK+VFWDAYHPTEAAN+I+AK LLDGD+T+++P NIR
Sbjct: 312 YFPPFACFKGPNQNSSQAACEDRSKFVFWDAYHPTEAANLIVAKALLDGDQTVATPFNIR 371

BLAST of MC06g1813 vs. TAIR 10
Match: AT2G23540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.7e-85
Identity = 154/343 (44.90%), Postives = 226/343 (65.89%), Query Frame = 0

Query: 28  TFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAK 87
           +F+FGDSLVD GNN+Y+ TLSRA+  P GIDFK SGG PTGRFTNGRTI DI+ E LG+ 
Sbjct: 49  SFIFGDSLVDAGNNNYLSTLSRANMKPNGIDFKASGGTPTGRFTNGRTIGDIVGEELGSA 108

Query: 88  SFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKV 147
           ++  PFLAP+ +   +  G+NYASG  GI++ TG +F+ R+ +  QV  F  +R+    +
Sbjct: 109 NYAIPFLAPDAKGKALLAGVNYASGGGGIMNATGRIFVNRLGMDVQVDFFNTTRKQFDDL 168

Query: 148 KGENETME-VLKNAIFSLTVGSNDII-NYIQPSIPIFQTNKASPSDYLDHMISNITIQLK 207
            G+ +  + + K +IFS+T+G+ND + NY+ P + +      +P D++  M+ ++  QL 
Sbjct: 169 LGKEKAKDYIAKKSIFSITIGANDFLNNYLFPLLSVGTRFTQTPDDFIGDMLEHLRDQLT 228

Query: 208 RLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQE 267
           RL+ + ARK V+  VGP+GCIP+ +T+     +EC +  N+L   YN RL  ++++LN++
Sbjct: 229 RLYQLDARKFVIGNVGPIGCIPYQKTINQLDENECVDLANKLANQYNVRLKSLLEELNKK 288

Query: 268 FNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGG---YFPPFVCYKGQNQNRSSSL 327
             P  MFV+AN Y + M++I NY +YGF  A + CCG    Y     C        +SSL
Sbjct: 289 L-PGAMFVHANVYDLVMELITNYDKYGFKSATKACCGNGGQYAGIIPC------GPTSSL 348

Query: 328 CDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
           C++R KYVFWD YHP+EAAN+IIAK+LL GD  + SP+N+ +L
Sbjct: 349 CEERDKYVFWDPYHPSEAANVIIAKQLLYGDVKVISPVNLSKL 384

BLAST of MC06g1813 vs. TAIR 10
Match: AT3G50400.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 1.0e-82
Identity = 157/346 (45.38%), Postives = 222/346 (64.16%), Query Frame = 0

Query: 28  TFVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAK 87
           +FVFGDSLVD GNN+Y+ TLSRA+SPP GIDFKPS G PTGRFTNGRTI+DI+ E LG +
Sbjct: 35  SFVFGDSLVDAGNNNYLQTLSRANSPPNGIDFKPSRGNPTGRFTNGRTIADIVGEKLGQQ 94

Query: 88  SFPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKV 147
           S+  P+LAPN   + +  G+NYASG  GIL+ TG +F+ R+ +  QV  F  +R+   K+
Sbjct: 95  SYAVPYLAPNASGEALLNGVNYASGGGGILNATGSVFVNRLGMDIQVDYFTNTRKQFDKL 154

Query: 148 KGENETME-VLKNAIFSLTVGSNDII-NYIQPSIPIFQTNKASPSDYLDHMISNITIQLK 207
            G+++  + + K ++FS+ +GSND + NY+ P +        +P  ++D MIS++  QLK
Sbjct: 155 LGQDKARDYIRKRSLFSVVIGSNDFLNNYLVPFVAAQARLTQTPETFVDDMISHLRNQLK 214

Query: 208 RLHGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQE 267
           RL+ + ARK VV  V P+GCIP+ +++    + +C +  N+L   YN RL +++    ++
Sbjct: 215 RLYDMDARKFVVGNVAPIGCIPYQKSINQLNDKQCVDLANKLAIQYNARLKDLLTVELKD 274

Query: 268 FNPKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCC------GGYFPPFVCYKGQNQNRS 327
                 FVYAN Y +FM +I N++ YGF  A + CC       G  P   C        +
Sbjct: 275 SLKDAHFVYANVYDLFMDLIVNFKDYGFRTASEACCETRGRLAGILP---C------GPT 334

Query: 328 SSLCDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
           SSLC DRSK+VFWDAYHPTEAAN++IA +LL GD    +P N+  L
Sbjct: 335 SSLCTDRSKHVFWDAYHPTEAANLLIADKLLYGDSKFVTPFNLLHL 371

BLAST of MC06g1813 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 9.6e-65
Identity = 138/345 (40.00%), Postives = 202/345 (58.55%), Query Frame = 0

Query: 29  FVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAKS 88
           FVFGDSLVD GNN+++ +L+R++  PYGIDF  +G QPTGRF+NG+TI D I E LG   
Sbjct: 51  FVFGDSLVDNGNNNHLNSLARSNYLPYGIDF--AGNQPTGRFSNGKTIVDFIGELLGLPE 110

Query: 89  FPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKVK 148
            P      +  VD ++ G+NYAS A GIL+ETG     R  +  QV+NFE++   + +  
Sbjct: 111 IPAFMDTVDGGVDILH-GVNYASAAGGILEETGRHLGERFSMGRQVENFEKTLMEISRSM 170

Query: 149 GENETMEVLKNAIFSLTVGSNDII-NYIQPSIPIFQTNKASPSDYLDHMISNITIQLKRL 208
            +    E +  ++  +++G+ND I NY++P +    ++   P+ + D ++SN T  L  L
Sbjct: 171 RKESVKEYMAKSLVVVSLGNNDYINNYLKPRL-FLSSSIYDPTSFADLLLSNFTTHLLEL 230

Query: 209 HGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQEFN 268
           +G G RK V+ GVGPLGCIP     Q A   EC E VN++ E +N RL  +VD+LN +  
Sbjct: 231 YGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRLNSDNK 290

Query: 269 --PKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGGYFPPFVCYKGQNQNRSSSL--- 328
              + +FVY N+Y   + I+ N   YGF    + CCG          G+N+   + L   
Sbjct: 291 TASEAIFVYGNTYGAAVDILTNPFNYGFEVTDRGCCG---------VGRNRGEITCLPLA 350

Query: 329 --CDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
             C  R ++VFWDA+HPT+A N+IIA    +G ++   PIN+ QL
Sbjct: 351 VPCAFRDRHVFWDAFHPTQAFNLIIALRAFNGSKSDCYPINLSQL 382

BLAST of MC06g1813 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.3e-64
Identity = 132/343 (38.48%), Postives = 199/343 (58.02%), Query Frame = 0

Query: 29  FVFGDSLVDVGNNDYIFTLSRADSPPYGIDFKPSGGQPTGRFTNGRTISDIIDEYLGAKS 88
           FV GDSLVD GNN+++ T++RA+  PYGID      QPTGRF+NG T  D++   L   S
Sbjct: 43  FVLGDSLVDAGNNNFLQTVARANFLPYGIDM---NYQPTGRFSNGLTFIDLLARLLEIPS 102

Query: 89  FPPPFLAPNTEVDTMYRGINYASGASGILDETGLLFLGRIPLREQVKNFEESRRYMVKVK 148
            PPPF  P T  + + +G+NYAS A+GILD +G  + GR  L +Q+ N E +   +  + 
Sbjct: 103 -PPPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLETTLSQLRTMM 162

Query: 149 GENETMEVLKNAIFSLTVGSNDII-NYIQPSIPIFQTNKASPSDYLDHMISNITIQLKRL 208
                 + L  ++  L  GSND I NY+ P++    + +  P D+ + ++S    QL  L
Sbjct: 163 SPQNFTDYLARSLVVLVFGSNDYINNYLMPNL-YDSSIRFRPPDFANLLLSQYARQLLTL 222

Query: 209 HGVGARKVVVVGVGPLGCIPFVRTLQFAPNDECSEEVNQLIEAYNFRLSEVVDQLNQEFN 268
           + +G RK+ + GV PLGCIP  R    +P D C + VNQ++  +N  L  +VDQLNQ  +
Sbjct: 223 YSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQR-S 282

Query: 269 PKTMFVYANSYAVFMQIIDNYQQYGFVDAKQPCCGGYFPPFVCYKGQNQNRSSSL----- 328
           P  ++VY N+Y+    I++N   YGF    + CCG          G+NQ + + L     
Sbjct: 283 PGAIYVYGNTYSAIGDILNNPAAYGFSVVDRACCG---------IGRNQGQITCLPLQTP 342

Query: 329 CDDRSKYVFWDAYHPTEAANIIIAKELLDGDETISSPINIRQL 366
           C +R++YVFWDA+HPT+ AN I+A+    G  + + P+N++Q+
Sbjct: 343 CPNRNQYVFWDAFHPTQTANSILARRAFYGPPSDAYPVNVQQM 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJ256.6e-13565.89GDSL esterase/lipase At5g41890 OS=Arabidopsis thaliana OX=3702 GN=At5g41890 PE=3... [more]
O234703.7e-8546.99GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3... [more]
O804702.4e-8444.90GDSL esterase/lipase At2g23540 OS=Arabidopsis thaliana OX=3702 GN=At2g23540 PE=2... [more]
Q9M2R91.4e-8145.38GDSL esterase/lipase At3g50400 OS=Arabidopsis thaliana OX=3702 GN=At3g50400 PE=2... [more]
Q9FNP21.4e-6340.00GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2... [more]
Match NameE-valueIdentityDescription
XP_022158401.17.00e-273100.00GDSL esterase/lipase At5g41890 [Momordica charantia][more]
XP_038907274.15.95e-22086.82GDSL esterase/lipase At5g41890 [Benincasa hispida][more]
XP_022977773.14.11e-21880.48GDSL esterase/lipase At5g41890 [Cucurbita maxima][more]
XP_004143942.32.97e-21681.18GDSL esterase/lipase At5g41890 [Cucumis sativus][more]
XP_023545021.13.74e-21479.68GDSL esterase/lipase At5g41890 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1E0T33.39e-273100.00GDSL esterase/lipase At5g41890 OS=Momordica charantia OX=3673 GN=LOC111024890 PE... [more]
A0A6J1IR231.99e-21880.48GDSL esterase/lipase At5g41890 OS=Cucurbita maxima OX=3661 GN=LOC111477974 PE=3 ... [more]
A0A0A0KM971.20e-21681.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148500 PE=3 SV=1[more]
A0A1S3AUU34.82e-21480.70GDSL esterase/lipase At5g41890 OS=Cucumis melo OX=3656 GN=LOC103483079 PE=3 SV=1[more]
A0A6J1GD955.19e-21479.41GDSL esterase/lipase At5g41890 OS=Cucurbita moschata OX=3662 GN=LOC111453124 PE=... [more]
Match NameE-valueIdentityDescription
AT5G41890.11.0e-13563.26GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT2G23540.11.7e-8544.90GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G50400.11.0e-8245.38GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G08460.19.6e-6540.00GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G71250.11.3e-6438.48GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 29..349
e-value: 7.6E-29
score: 101.8
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 27..354
e-value: 1.1E-76
score: 260.2
NoneNo IPR availablePANTHERPTHR45648GDSL LIPASE/ACYLHYDROLASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_4G14700)coord: 8..367
NoneNo IPR availablePANTHERPTHR45648:SF25GDSL-LIKE LIPASE/ACYLHYDROLASE SUPERFAMILY PROTEINcoord: 8..367
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 157..350
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 29..353
e-value: 2.37833E-121
score: 351.147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1813.1MC06g1813.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds