MC06g1803 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1803
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionNon-specific serine/threonine protein kinase
LocationMC06: 25294586 .. 25298420 (+)
RNA-Seq ExpressionMC06g1803
SyntenyMC06g1803
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTTAAGCATATAGATTAAATTAGAGGTTAGGTTCAAATTATCATCTCGCGAATGGTTGATTTTTTTTTTTAGTGGAATTTATCGAAAAGATAATTACAATAAAGTTTATCAAAAAGAAAAAGAGAAAAAACAGATATTCATTCATATTGTCAATGTTTATCAGGTTATGGTTAAAATAGTGAAAAAAAAGGTAGAGGAAGGGTGGCGGAGCTAAATTCAGTGTTTGAACAGTTGAATTGAATTGTGAAATTATGAATTGTGAATTGTGAATTTGGGGACGACGACGACTCCAACAAACAATTCAATTCAATCGCATCCTCTCTGGGTTTTCTTCTCCCTTCCAATTTAGGGTTTCCTTTTCCGATTTCGGGGAGGAACCAATTTGAGGTATGCGGATGCTTATTATATCCCCCAACCCATGATTCCTTTTGCGATGTGTACAATCTCTGCTCATCCCTTTTCTTCTGCTCTCCGCTTGCAGTCGCCTATGGAGTTGATGTTCATGGGCTCCCGATTCCACTTCCACTCCGCTTTCGACGCCGCTTCCGAGGCCTTTTCTGAACGCCTTCACGCCCTCATTCTCATTGTTCTTCTCTGACTCACTGGGTTCTCCATTATTGTTCCCCCCCTCTTCCCTTCTTCTTCTCTCCAATCTGCCTGTGTTGCTGCGATGGATTTGATGTCTGGGAATTCTGAGCCTGAAGATCGTGAGGTTGCGGAGAAAGATCCGACGGGACGGTACATACGGGTATTTTTTTTAACCAGCTCTTCCTTTGTTGTTTTTCGGCGATCCATGGTTAGGTTTTTGAATTTTGTCATCAGCTCGGGATTTGCCCTAGCTACTGGCTGGATTGTAGGAAGGGGAGAATTTTGCACTTTATATTTCTACTTTTTAACTTTTTATTTGGGGATTCGCTGCTGATTACAGATTTAGTTGGTTTGTTTTACTTTTGTAAGCACAAATTTGTGTTACCTTTATTAACCGTGATTAACCACTAAACTCGATATTGTTCATTGGATTGGTGGTTTCGTCGCTAATGGTTTGATAATCGACTACTTATGTTATGGATGTGTTTTTTGGTTCTGCTTTATTGTGTAGATGTTTTCTCCAATTTGTCTTTATGATTCAGTCCTCATTTTTACTTTTCTTTTAACTTTTCAGTACGATGAAATTTTGGGTAAAGGAGCATTCAAGACAGTGTATGCTTCTGATATCTATCAATCTCAATCCTTTTATTCTTTCTGCTAGTCTGTTACATCAGTTTTTAGTTGAGTACCTTCTCAAACAAGTAAGGGTATATTATTTGACTTGTGGTCTGTTTATGTTGTTGTCGACTCTTCAGATACAAAGCATTCGACGAAGTCGGTGGAATAGAAGTTGCATGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCGGAACAACTCCAACGATTATATTCTGAGGTTCATCTCCTAAAGTCACTGAAACATGAAAATATCATCAAGTTCCATAGCTACTGGGTTGATGATGAGAACAAGACTATCAACATGATAACAGAATTGTTCACTTCTGGGAGTTTGAGGCAGTAAGATCCAGAGTCGTCTTTTTATTTCTGTAATGTGTTTATTGTTATCATTTAACATTTTCTCATTTATGGTATACGTTGGAATTCAGATATCGAAAGAAGCACCGTAAAGTTGACATGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGGGGTTTAACCTATTTGCATGGTCACAATCCTCCAATTATTCACAGGGATTTGAAATGCGATAATATATTTGTTAACGGAAATACTGGAGAAGTTAAAATTGGAGATCTCGGATTGGCAATTGTCATGCAGCAGTCTACTGCTCGAAGTGTTATTGGTAATAATCTCATTTTCAGGCTCTGAGTTCTTTTTGTGGGGATAGTATTCTTGATCACTTCAACTGATATTTGAGACGTGTGATTATTATTCAGGCACTCCAGAGTTCATGGCCCCAGAGCTTTATGATGAGGACTATAATGAACTTGTCGACATATATTCTTTTGGCATGTGCATGTTGGAAATAGTTACGTGTGAATACCCATATAATGAATGTAAAAATGCTGCTCAAATATTCAAGAAGGTTACTTCTGTAAGTAAATGATTATTTCTATCAAATTATCTCTTGTCCATTTAATACGTCCATCAAATTGAGATCCTGAAATCATTTCCTCTTTCTTGCAGGGTACAAAGCCTGCTTCCCTTGAAAAAGTGTTGGATCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCATTCCTTGTGATTGAAAATACTAAGGAGCATAGTTCCGAACCTTCAAGGTTATTGAATGCGCAGTTCATATCGGTAAATCCTCCACTGCTTGGTTCCAATCCAGTGGACATAGCTCATAGCTGCATGAAACTCTCAGGCTCAGAGAGCTCATCTGCCAAAAGCAGTAATGAAATTTCTCACTTTTCAACTCCGGAGCTCTCTGCCAAAAGCAGTAATGAAATTTCTCACTTTTCAACTCTGGAGCTCACTGCCAAAAGCATTAATGAAGTTTCCCACTTTTCAAGTTTGGAACTTCAGAGATTCACTGAGAATAATGAATTGATGTTGAAAGGGGAAATGACGGATCATAATACAATTTCATTCCATTTGCGCATTGCTGAACAATGTGGTAAATTTTTTGGCCAACCTTTCATGAATCTTCTAAAAGCTCTTTTTTGTTTAGCGAGTGTGTTAGTACTCTTCATGGTAACTTGAGCATTTATTTTCATGCAGGTAAATCGAAGAATATCCATTTTGCCTTTTACCTTGATTCTGATACTGCCTTGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTACCATAGCCAAACTAATAGATGAGTTAATTACCAAGTTTGTTCCCAGCTGGAAACCGTGTGCAAATATTTGCTCTGAAACTCAAGAGGATAAGACCTTCATTCCTCCTTTCTTCCCTGAGCTTGTACTGTCATCTCCCATGGTGGCTGACGCTAGGAAATCTCTGACACATTTGGCCGACATGGAAGATCAAGACAACCAGCAGTCGATTATCTCGTGCGCATCACTGGATTACATCCATTCGACAACCTCAGGTTACAGCACTGGAAAAGGATCAGAATACGGCGACTTCGGCCACCCTGAAGGCGAAAAAGTGTTTATCAGCGAACCAATTGATTCTGACACCGCAGTTTCCGTCTCGACTGTGATAGATTTTGCGAAACCGTCTCTAATCTCCTCCTGCTCAGGAATGTCTAAAGATTTGAGTTTGTCGAGCTTTTCCTCGTTTTCTGTGGAAGAAAGAGATCACCATGATGAACTGAAGCTGGAGATTGATGCTATTGACTTGCAGTACCACCAGTGCCTTTGTGAACTCTCAAGAATGAGAGAGGAAGCAATAGAAAGTGTCAAGAAGAGGTGGATGGCAAAGAAGAAAGCTTGTGCTATTTGAAGCACAGCCCCCAATTTCCCAAGGATTTACAATGTTTTCTTTTGGCAACAATTGAAGATTGTCTTATTTTTTTAATACTGACATTTATTCTTTACATTCTATCCAACCAAGTCCTAACTCCTAACTTCTGTTCTCACCCCACAGTCCTTTCATTACATAGTTTAGACATATTCATTGCGTTGCATTTGTTCCACATTCTTCCCCATGTGCAACTTTTTTAAACTCTATATTTTCCATGTATTCTTTCACTTTGGCATGAGCTCTCTCTCTTTCTGATCTTTTCC

mRNA sequence

CGTTAAGCATATAGATTAAATTAGAGGTTAGGTTCAAATTATCATCTCGCGAATGGTTGATTTTTTTTTTTAGTGGAATTTATCGAAAAGATAATTACAATAAAGTTTATCAAAAAGAAAAAGAGAAAAAACAGATATTCATTCATATTGTCAATGTTTATCAGGTTATGGTTAAAATAGTGAAAAAAAAGGTAGAGGAAGGGTGGCGGAGCTAAATTCAGTGTTTGAACAGTTGAATTGAATTGTGAAATTATGAATTGTGAATTGTGAATTTGGGGACGACGACGACTCCAACAAACAATTCAATTCAATCGCATCCTCTCTGGGTTTTCTTCTCCCTTCCAATTTAGGGTTTCCTTTTCCGATTTCGGGGAGGAACCAATTTGAGTCGCCTATGGAGTTGATGTTCATGGGCTCCCGATTCCACTTCCACTCCGCTTTCGACGCCGCTTCCGAGGCCTTTTCTGAACGCCTTCACGCCCTCATTCTCATTGTTCTTCTCTGACTCACTGGGTTCTCCATTATTGTTCCCCCCCTCTTCCCTTCTTCTTCTCTCCAATCTGCCTGTGTTGCTGCGATGGATTTGATGTCTGGGAATTCTGAGCCTGAAGATCGTGAGGTTGCGGAGAAAGATCCGACGGGACGGTACATACGGTACGATGAAATTTTGGGTAAAGGAGCATTCAAGACAGTATACAAAGCATTCGACGAAGTCGGTGGAATAGAAGTTGCATGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCGGAACAACTCCAACGATTATATTCTGAGGTTCATCTCCTAAAGTCACTGAAACATGAAAATATCATCAAGTTCCATAGCTACTGGGTTGATGATGAGAACAAGACTATCAACATGATAACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAAGAAGCACCGTAAAGTTGACATGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGGGGTTTAACCTATTTGCATGGTCACAATCCTCCAATTATTCACAGGGATTTGAAATGCGATAATATATTTGTTAACGGAAATACTGGAGAAGTTAAAATTGGAGATCTCGGATTGGCAATTGTCATGCAGCAGTCTACTGCTCGAAGTGTTATTGGCACTCCAGAGTTCATGGCCCCAGAGCTTTATGATGAGGACTATAATGAACTTGTCGACATATATTCTTTTGGCATGTGCATGTTGGAAATAGTTACGTGTGAATACCCATATAATGAATGTAAAAATGCTGCTCAAATATTCAAGAAGGTTACTTCTGGTACAAAGCCTGCTTCCCTTGAAAAAGTGTTGGATCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCATTCCTTGTGATTGAAAATACTAAGGAGCATAGTTCCGAACCTTCAAGGTTATTGAATGCGCAGTTCATATCGGTAAATCCTCCACTGCTTGGTTCCAATCCAGTGGACATAGCTCATAGCTGCATGAAACTCTCAGGCTCAGAGAGCTCATCTGCCAAAAGCATTTCCCACTTTTCAAGTTTGGAACTTCAGAGATTCACTGAGAATAATGAATTGATGTTGAAAGGGGAAATGACGGATCATAATACAATTTCATTCCATTTGCGCATTGCTGAACAATGTGGTAAATCGAAGAATATCCATTTTGCCTTTTACCTTGATTCTGATACTGCCTTGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTACCATAGCCAAACTAATAGATGAGTTAATTACCAAGTTTGTTCCCAGCTGGAAACCGTGTGCAAATATTTGCTCTGAAACTCAAGAGGATAAGACCTTCATTCCTCCTTTCTTCCCTGAGCTTGTACTGTCATCTCCCATGGTGGCTGACGCTAGGAAATCTCTGACACATTTGGCCGACATGGAAGATCAAGACAACCAGCAGTCGATTATCTCGTGCGCATCACTGGATTACATCCATTCGACAACCTCAGGTTACAGCACTGGAAAAGGATCAGAATACGGCGACTTCGGCCACCCTGAAGGCGAAAAAGTGTTTATCAGCGAACCAATTGATTCTGACACCGCAGTTTCCGTCTCGACTGTGATAGATTTTGCGAAACCGTCTCTAATCTCCTCCTGCTCAGGAATGTCTAAAGATTTGAGTTTGTCGAGCTTTTCCTCGTTTTCTGTGGAAGAAAGAGATCACCATGATGAACTGAAGCTGGAGATTGATGCTATTGACTTGCAGTACCACCAGTGCCTTTGTGAACTCTCAAGAATGAGAGAGGAAGCAATAGAAAGTGTCAAGAAGAGGTGGATGGCAAAGAAGAAAGCTTGTGCTATTTGAAGCACAGCCCCCAATTTCCCAAGGATTTACAATGTTTTCTTTTGGCAACAATTGAAGATTGTCTTATTTTTTTAATACTGACATTTATTCTTTACATTCTATCCAACCAAGTCCTAACTCCTAACTTCTGTTCTCACCCCACAGTCCTTTCATTACATAGTTTAGACATATTCATTGCGTTGCATTTGTTCCACATTCTTCCCCATGTGCAACTTTTTTAAACTCTATATTTTCCATGTATTCTTTCACTTTGGCATGAGCTCTCTCTCTTTCTGATCTTTTCC

Coding sequence (CDS)

ATGGATTTGATGTCTGGGAATTCTGAGCCTGAAGATCGTGAGGTTGCGGAGAAAGATCCGACGGGACGGTACATACGGTACGATGAAATTTTGGGTAAAGGAGCATTCAAGACAGTATACAAAGCATTCGACGAAGTCGGTGGAATAGAAGTTGCATGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCGGAACAACTCCAACGATTATATTCTGAGGTTCATCTCCTAAAGTCACTGAAACATGAAAATATCATCAAGTTCCATAGCTACTGGGTTGATGATGAGAACAAGACTATCAACATGATAACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAAGAAGCACCGTAAAGTTGACATGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGGGGTTTAACCTATTTGCATGGTCACAATCCTCCAATTATTCACAGGGATTTGAAATGCGATAATATATTTGTTAACGGAAATACTGGAGAAGTTAAAATTGGAGATCTCGGATTGGCAATTGTCATGCAGCAGTCTACTGCTCGAAGTGTTATTGGCACTCCAGAGTTCATGGCCCCAGAGCTTTATGATGAGGACTATAATGAACTTGTCGACATATATTCTTTTGGCATGTGCATGTTGGAAATAGTTACGTGTGAATACCCATATAATGAATGTAAAAATGCTGCTCAAATATTCAAGAAGGTTACTTCTGGTACAAAGCCTGCTTCCCTTGAAAAAGTGTTGGATCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACTACCTGCAAGTGAACTCTTGAAAGATCCATTCCTTGTGATTGAAAATACTAAGGAGCATAGTTCCGAACCTTCAAGGTTATTGAATGCGCAGTTCATATCGGTAAATCCTCCACTGCTTGGTTCCAATCCAGTGGACATAGCTCATAGCTGCATGAAACTCTCAGGCTCAGAGAGCTCATCTGCCAAAAGCATTTCCCACTTTTCAAGTTTGGAACTTCAGAGATTCACTGAGAATAATGAATTGATGTTGAAAGGGGAAATGACGGATCATAATACAATTTCATTCCATTTGCGCATTGCTGAACAATGTGGTAAATCGAAGAATATCCATTTTGCCTTTTACCTTGATTCTGATACTGCCTTGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTACCATAGCCAAACTAATAGATGAGTTAATTACCAAGTTTGTTCCCAGCTGGAAACCGTGTGCAAATATTTGCTCTGAAACTCAAGAGGATAAGACCTTCATTCCTCCTTTCTTCCCTGAGCTTGTACTGTCATCTCCCATGGTGGCTGACGCTAGGAAATCTCTGACACATTTGGCCGACATGGAAGATCAAGACAACCAGCAGTCGATTATCTCGTGCGCATCACTGGATTACATCCATTCGACAACCTCAGGTTACAGCACTGGAAAAGGATCAGAATACGGCGACTTCGGCCACCCTGAAGGCGAAAAAGTGTTTATCAGCGAACCAATTGATTCTGACACCGCAGTTTCCGTCTCGACTGTGATAGATTTTGCGAAACCGTCTCTAATCTCCTCCTGCTCAGGAATGTCTAAAGATTTGAGTTTGTCGAGCTTTTCCTCGTTTTCTGTGGAAGAAAGAGATCACCATGATGAACTGAAGCTGGAGATTGATGCTATTGACTTGCAGTACCACCAGTGCCTTTGTGAACTCTCAAGAATGAGAGAGGAAGCAATAGAAAGTGTCAAGAAGAGGTGGATGGCAAAGAAGAAAGCTTGTGCTATTTGA

Protein sequence

MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKSISHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI
Homology
BLAST of MC06g1803 vs. ExPASy Swiss-Prot
Match: Q944Q0 (Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=1 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 7.0e-158
Identity = 316/604 (52.32%), Postives = 401/604 (66.39%), Query Frame = 0

Query: 8   SEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQ 67
           S  E+ + AEKDP+GRYIRYD++LG+GAFKTVYKAFDEV GIEVAW+ V IEDV+Q P Q
Sbjct: 12  SSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIEDVMQMPGQ 71

Query: 68  LQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKVDMKA 127
           L+RLYSEVHLLK+LKHENIIK    WVD++NKTINMITELFTSGSLR YRKKHRKVD KA
Sbjct: 72  LERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPKA 131

Query: 128 FKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQSTARSV 187
            KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTGEVKIGDLGLA V+QQ TARSV
Sbjct: 132 IKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARSV 191

Query: 188 IGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGTKPAS 247
           IGTPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPYNEC+N AQI+KKVTS  KP S
Sbjct: 192 IGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQS 251

Query: 248 LEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQFISVNP 307
           L KV DPQV+QFIEKCL+PAS R  A EL KDPFL  +  K+ +   S   ++++  V P
Sbjct: 252 LGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSALLASSSTSSKY--VRP 311

Query: 308 PLLGSNPVDIAHSCMKLSGSESSSAKSISHFSSLELQRFTENNELMLKGEMTDHNTISFH 367
           P L   P+D+ H+         SS +      ++ELQR  EN E  L+GE +D  T S  
Sbjct: 312 PQLEHLPMDVDHN----ENKSVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVTASMV 371

Query: 368 LRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSW 427
           LRIA+  GK + +HFAFYL+SDTA AIA EMVE+L L++++   IA +ID+ I + +   
Sbjct: 372 LRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQLL--- 431

Query: 428 KPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTHLADMEDQDNQ---QSIISCA 487
                       D+T           +SP +        +   +  +D +   QS+ S  
Sbjct: 432 -----------SDRT-----SSHHNQNSPRLTHEDHEAANQQTVNSKDEEAAGQSMKSDI 491

Query: 488 SLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSC 547
           S DY       YS   G+   + G                 A S+S+ +D        SC
Sbjct: 492 SADYYFP----YSANDGNAAMEAGR---------------DAESMSSYLD--------SC 551

Query: 548 SGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWM 607
           S M      S+  + S+ + D+ ++LK E++ I+ Q++Q   +L +++E+AIE+ K++W+
Sbjct: 552 SMM------STIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKLKEDAIENAKRKWI 557

Query: 608 AKKK 609
            KK+
Sbjct: 612 TKKQ 557

BLAST of MC06g1803 vs. ExPASy Swiss-Prot
Match: Q6EU49 (Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK4 PE=2 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 8.9e-153
Identity = 316/636 (49.69%), Postives = 398/636 (62.58%), Query Frame = 0

Query: 1   MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIED 60
           M++     + +D E  E DPT RYIRY+E+LG+GA KTVYKAFDEV GIEVAWSQV+I++
Sbjct: 1   MEVFGDLDKVDDAECVEVDPTRRYIRYNEVLGRGAMKTVYKAFDEVEGIEVAWSQVEIDE 60

Query: 61  VLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKH 120
           V+QSP+ L+RLYSEVHLLKSLKHEN++KF++YWVDD+ KTIN+ITELFTSGSLRQYR+KH
Sbjct: 61  VMQSPDNLERLYSEVHLLKSLKHENVMKFYNYWVDDQKKTINVITELFTSGSLRQYRQKH 120

Query: 121 RKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQ 180
            +VD+KA KNWARQ+LRGL YLH H PPIIHRDLKCDNIFVNGN GEVKIGDLGLA VM 
Sbjct: 121 PRVDLKAIKNWARQVLRGLDYLHTHQPPIIHRDLKCDNIFVNGNHGEVKIGDLGLATVML 180

Query: 181 QSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVT 240
              A+SVIGTPEFMAPELYDE+Y+ELVDIYSFGMCMLE+ T EYPY+EC NAAQIFKKV+
Sbjct: 181 TPRAKSVIGTPEFMAPELYDENYDELVDIYSFGMCMLEMFTLEYPYSECTNAAQIFKKVS 240

Query: 241 SGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNA 300
            G KPA+L K+ + Q KQFI+KCLVPAS RL A ELL+DPFL  +N+       S L+  
Sbjct: 241 KGVKPAALAKITNIQAKQFIDKCLVPASERLSAKELLQDPFLCSDNS-------SVLVGT 300

Query: 301 QFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKSIS-------HFSSLELQRFTENNELM 360
           +F S  P    S  V +    M +  +ES    +         H S LE  R  +N EL 
Sbjct: 301 KFPSSLPK---SVDVSLEALHMDVDTNESMCTSTCKRNDLGGPHRSVLEFTRTNKNTELK 360

Query: 361 LKGEMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIA 420
           L GE  D N++S  LRIA+ CG ++NIHF FYLDSDTA+++A EMVEQLEL++ D T IA
Sbjct: 361 LTGEKLDDNSVSLVLRIADLCGHARNIHFLFYLDSDTAMSVAAEMVEQLELADCDVTFIA 420

Query: 421 KLIDELITKFVPSWKPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTHLADMED 480
             ID LI   VP  +   +    T  +         E V++S         LTH   + +
Sbjct: 421 DFIDLLIVNLVPGQQLMNDAVMSTSSESKMGE---SEHVITS---QQHPSELTHDYVLVE 480

Query: 481 QDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVI 540
                   + +  DYI S  +  + G        G    E   IS  +D  +       +
Sbjct: 481 GMMHSKEANASPSDYIDSLLNATNLG--------GPNSSEGSDISVQLDGSSKSLSEYGV 540

Query: 541 DFAKPSLISSCSGMSK-------DLSLSSFSSFSVEERDHHDELKL-------------- 600
           D  +     +  G  K           S+F+ F +++  HH EL +              
Sbjct: 541 DEYRTLECGAYKGTDKLGCRHPLSNGSSNFAIFQMDQASHHSELVIGASVSITENRDVLN 600

Query: 601 -EIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKK 608
            E+  I+ QY Q   EL+RMREEA+E  +K+W+  K
Sbjct: 601 GELGLIEAQYEQWFRELTRMREEALEGARKKWLPDK 612

BLAST of MC06g1803 vs. ExPASy Swiss-Prot
Match: Q8RXE5 (Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 GN=WNK10 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 9.5e-147
Identity = 304/600 (50.67%), Postives = 370/600 (61.67%), Query Frame = 0

Query: 11  EDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQR 70
           E+ +  +KDPTGRYIRY+++LG+GAFKTVYKAFDEV GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 71  LYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKVDMKAFKN 130
           LYSEVHLL SLKH+NIIK    WVDD NK+INMITELFTSGSL  YRKKHRKVD KA  N
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 131 WARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQSTARSVIGT 190
           WARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG+VKIGDLGLA VMQQ TARSVIGT
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 191 PEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGTKPASLEK 250
           PEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSG KP SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 251 VLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQFISVNPPLL 310
           V DPQVKQFIEKCL+PA  R  A ELLKD  L ++  K+ +   S   N  F    PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTLTASS--NTTFKPAMPPQC 301

Query: 311 GSNPVDIAHSCMKLSGSESSSAKSISH---FSSLELQRFTENNELMLKGEMTDHNTISFH 370
              P+D+ +     S S  SSAKS        ++E+QR  E+ E  L GE  D    S  
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 371 LRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSW 430
           LRIA   G+++ + F F L +DTA A+  EMVE+L+LS+ + T IA++IDELI K     
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMKL---- 421

Query: 431 KPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTH---LADMEDQDNQQSIISCA 490
                                            A +SL +   +   +D++  +S+ S  
Sbjct: 422 --------------------------------KANRSLPNANSVYQSKDEEAGESMKSEI 481

Query: 491 SLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSC 550
           S DY H      S+ +GS  G                           ++    S + SC
Sbjct: 482 SADYYHRV----SSNEGSRLG----------------------CCCEAVESLLSSFLDSC 521

Query: 551 SGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWM 605
           S +S   S               ++LK E++ I+ QY+Q    L RM+EEAIE  K++WM
Sbjct: 542 SMVSNKQS---------------EDLKTELNVIESQYNQSCQRLLRMKEEAIEKAKRKWM 521

BLAST of MC06g1803 vs. ExPASy Swiss-Prot
Match: Q8S8Y8 (Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN=WNK6 PE=2 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 7.6e-144
Identity = 266/435 (61.15%), Postives = 322/435 (74.02%), Query Frame = 0

Query: 2   DLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDV 61
           D  S   EP D EV E DPT RYIRY E++GKGAFKTVYKAFDEV GIEVAW+QV I+DV
Sbjct: 5   DDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDV 64

Query: 62  LQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHR 121
           LQSP  L+RLYSEV LLKSLKH NII+F++ W+DD+NKT+N+ITELFTSGSLR YRKKHR
Sbjct: 65  LQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHR 124

Query: 122 KVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 181
           KV+MKA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q
Sbjct: 125 KVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQ 184

Query: 182 STARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTS 241
           + A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKN+AQI+KKV+S
Sbjct: 185 ANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSS 244

Query: 242 GTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHS--SEPSRLLN 301
           G KPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL +     ++    P  ++ 
Sbjct: 245 GIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMP 304

Query: 302 AQFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKSISHFSS------LELQRFTENNELM 361
            +    +  L+   P     S       +  S   I  FS       +E++R    N  +
Sbjct: 305 KEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSRCIEVRRAKRGNFFV 364

Query: 362 LKGEMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIA 421
           LKGE  D  ++S  LRI ++ G+ +NIHF FY + DTA  ++ EMVEQLEL++++ T IA
Sbjct: 365 LKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTFIA 424

Query: 422 KLIDELITKFVPSWK 429
           +LID L+   +P+WK
Sbjct: 425 ELIDILLVNMIPTWK 439

BLAST of MC06g1803 vs. ExPASy Swiss-Prot
Match: Q8LST2 (Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN=WNK7 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 3.3e-139
Identity = 286/606 (47.19%), Postives = 382/606 (63.04%), Query Frame = 0

Query: 9   EPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQL 68
           EP D EV E DPT RYIRY E++GKGA KTV+K FDEV GIEVAW+QV I+D+LQSP+ L
Sbjct: 12  EPPDPEVLEIDPTCRYIRYKEVIGKGASKTVFKGFDEVDGIEVAWNQVRIDDLLQSPDCL 71

Query: 69  QRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKVDMKAF 128
           +RLYSEV LLKSLKH+NII+F++ W+DD+NKT+N+ITELFTSGSLRQYRKKHRKV+MKA 
Sbjct: 72  ERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYRKKHRKVNMKAV 131

Query: 129 KNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQSTARSVI 188
           K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN GEVKIGDLGLA VM+Q+ A+SVI
Sbjct: 132 KCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLATVMEQANAKSVI 191

Query: 189 GTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGTKPASL 248
           GTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT EYPY EC+N+AQI+KKV+SG KPASL
Sbjct: 192 GTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYKKVSSGIKPASL 251

Query: 249 EKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHS--SEPSRLLNAQFISVN 308
            KV DP+V +FIEKCL+PAS RL A ELL D FL +     ++    P  ++  +     
Sbjct: 252 SKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPDIVMPKEGSFGE 311

Query: 309 PPLLGSNPVDIAHSCMKLSGSESSS-----AKSISHFSSLELQRFTENNELMLKGEMTDH 368
             L+   P +  +  M ++  E ++     + + S  + +E++R    N  +LKGE  D 
Sbjct: 312 RCLMSEGPPNARNRTMSMNLDEDNNLPIVISSNNSGTNCIEVRRAKRGNFFVLKGEENDE 371

Query: 369 NTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDELIT 428
           N++S  LRI ++ G+ +NIHF F+ + DTA  ++ EMVEQLEL++++   IA+LID L+ 
Sbjct: 372 NSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELTDKNVKFIAELIDVLLV 431

Query: 429 KFVPSWKPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTHLADMEDQDNQQSII 488
             +P+WK                                   ++ HL  +  Q NQ    
Sbjct: 432 NLIPNWK--------------------------------TDVAVDHL--IHPQQNQ---- 491

Query: 489 SCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLI 548
                    S+   +  G  S+ G+          IS  + SD         D A P++ 
Sbjct: 492 ---------SSKDNHQNGASSQAGE---------SISHSLSSDYCPRSD---DEANPTVA 551

Query: 549 SSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKK 608
           ++      +         S+EE +  + LK E++ I+ ++ + + E++R REEA    K 
Sbjct: 552 ATTEDQEAE------KPGSLEEEEEDERLKEELEKIEERFREEMKEITRKREEATMETKN 552

BLAST of MC06g1803 vs. NCBI nr
Match: XP_022159288.1 (probable serine/threonine-protein kinase WNK10 [Momordica charantia])

HSP 1 Score: 1194 bits (3088), Expect = 0.0
Identity = 611/647 (94.44%), Postives = 612/647 (94.59%), Query Frame = 0

Query: 1   MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIED 60
           MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIED
Sbjct: 1   MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIED 60

Query: 61  VLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKH 120
           VLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKH
Sbjct: 61  VLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKH 120

Query: 121 RKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQ 180
           RKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQ
Sbjct: 121 RKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQ 180

Query: 181 QSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVT 240
           QSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVT
Sbjct: 181 QSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVT 240

Query: 241 SGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNA 300
           SGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNA
Sbjct: 241 SGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNA 300

Query: 301 QFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS-------------------------- 360
           QFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS                          
Sbjct: 301 QFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKSSNEISHFSTPELSAKSSNEISHFSTL 360

Query: 361 ---------ISHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFY 420
                    +SHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFY
Sbjct: 361 ELTAKSINEVSHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFY 420

Query: 421 LDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANICSETQEDKTFIP 480
           LDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANICSETQEDKTFIP
Sbjct: 421 LDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANICSETQEDKTFIP 480

Query: 481 PFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYG 540
           PFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYG
Sbjct: 481 PFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYG 540

Query: 541 DFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERD 600
           DFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERD
Sbjct: 541 DFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERD 600

Query: 601 HHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI 612
           HHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI
Sbjct: 601 HHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI 647

BLAST of MC06g1803 vs. NCBI nr
Match: KAA0042657.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa] >TYK06059.1 serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1013 bits (2620), Expect = 0.0
Identity = 523/628 (83.28%), Postives = 559/628 (89.01%), Query Frame = 0

Query: 3   LMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 62
           +MS NSE +DRE+ EKDP+GRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL
Sbjct: 8   IMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 67

Query: 63  QSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRK 122
           QSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD++KTINMITELFTSGSLRQYRKKHRK
Sbjct: 68  QSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRK 127

Query: 123 VDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQS 182
           VD+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 
Sbjct: 128 VDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQP 187

Query: 183 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSG 242
           TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG
Sbjct: 188 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG 247

Query: 243 TKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQF 302
            KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLV E+ KEHSSE SR LN QF
Sbjct: 248 IKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQF 307

Query: 303 ISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEMT 362
            SVNPPLLGS+P+D  H C KLSGS +SS KS   ISHFS+ ELQR TENNEL LKGEMT
Sbjct: 308 KSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMT 367

Query: 363 DHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDEL 422
           DHNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDAT IAKLIDEL
Sbjct: 368 DHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDEL 427

Query: 423 ITKFVPSWKPCANIC-----------SETQEDKTFIPPFFPELVLSSPMVADARKSLTHL 482
           I KFVPSWKPC N C           SE QE+K+FI PFF ELVLSSPMV  AR +LT L
Sbjct: 428 IAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGL 487

Query: 483 ADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISE-PIDSDTAV 542
           A +EDQ+NQQSIISCAS++YI+ST S YS GKGSE G+FGHP+ EK +IS   ID D   
Sbjct: 488 AKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEA 547

Query: 543 ---SVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQC 602
              ++ST+IDFAKPSLISSCSGMSK+LSLSSFS+ S+EERDH DELK+EIDAIDLQYHQC
Sbjct: 548 VGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQC 607

Query: 603 LCELSRMREEAIESVKKRWMAKKKACAI 612
           LCELSRMREEAIES KKRWM+KKKA  I
Sbjct: 608 LCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of MC06g1803 vs. NCBI nr
Match: XP_008437436.1 (PREDICTED: serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo])

HSP 1 Score: 1013 bits (2618), Expect = 0.0
Identity = 523/628 (83.28%), Postives = 558/628 (88.85%), Query Frame = 0

Query: 3   LMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 62
           +MS NSE +DRE+ EKDP+GRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL
Sbjct: 8   IMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 67

Query: 63  QSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRK 122
           QSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD++KTINMITELFTSGSLRQYRKKHRK
Sbjct: 68  QSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRK 127

Query: 123 VDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQS 182
           VD+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 
Sbjct: 128 VDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQP 187

Query: 183 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSG 242
           TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG
Sbjct: 188 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG 247

Query: 243 TKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQF 302
            KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLV E+ KEHSSE SR LN QF
Sbjct: 248 IKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQF 307

Query: 303 ISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEMT 362
            SVNPPLLGS+P+D  H C KLSGS +SS KS   ISHFS+ ELQR TENNEL LKGEMT
Sbjct: 308 KSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMT 367

Query: 363 DHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDEL 422
           DHNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDAT IAKLIDEL
Sbjct: 368 DHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDEL 427

Query: 423 ITKFVPSWKPCANIC-----------SETQEDKTFIPPFFPELVLSSPMVADARKSLTHL 482
           I KFVPSWKPC N C           SE QE+K+FI PFF ELVLSSPMV  AR +LT L
Sbjct: 428 IAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGL 487

Query: 483 ADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISE-PIDSDTAV 542
           A +EDQ+NQQSIISCAS +YI+ST S YS GKGSE G+FGHP+ EK +IS   ID D   
Sbjct: 488 AKVEDQENQQSIISCASAEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEA 547

Query: 543 ---SVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQC 602
              ++ST+IDFAKPSLISSCSGMSK+LSLSSFS+ S+EERDH DELK+EIDAIDLQYHQC
Sbjct: 548 VGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQC 607

Query: 603 LCELSRMREEAIESVKKRWMAKKKACAI 612
           LCELSRMREEAIES KKRWM+KKKA  I
Sbjct: 608 LCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of MC06g1803 vs. NCBI nr
Match: XP_004143946.1 (serine/threonine-protein kinase WNK8 isoform X1 [Cucumis sativus] >KGN49991.1 hypothetical protein Csa_000022 [Cucumis sativus])

HSP 1 Score: 1005 bits (2598), Expect = 0.0
Identity = 516/626 (82.43%), Postives = 556/626 (88.82%), Query Frame = 0

Query: 3   LMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 62
           +MSGNSE +DR + EKDP+GRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL
Sbjct: 54  IMSGNSEADDRGIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 113

Query: 63  QSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRK 122
           QSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD++KTINMITELFTSGSLRQYRKKHRK
Sbjct: 114 QSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRK 173

Query: 123 VDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQS 182
           VD+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 
Sbjct: 174 VDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQP 233

Query: 183 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSG 242
           TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG
Sbjct: 234 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG 293

Query: 243 TKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQF 302
            KPASLEKVLDPQVKQFIEKCLVPAS RLPASELLKDPFL  E+ K++SSE SR LN  F
Sbjct: 294 IKPASLEKVLDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSELSRSLNEHF 353

Query: 303 ISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEMT 362
            SVNPPLLGS+P++  H+C KLSGS +SS KS   ISHFS+ ELQR TENNEL LKG+MT
Sbjct: 354 KSVNPPLLGSHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGDMT 413

Query: 363 DHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDEL 422
           DHNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDAT IAKLIDEL
Sbjct: 414 DHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDEL 473

Query: 423 ITKFVPSWKPCANICSE----------TQEDKTFIPPFFPELVLSSPMVADARKSLTHLA 482
           I KFVPSWKPC N C E           QEDKTFI PFF ELVLSSPMVA AR +LT LA
Sbjct: 474 IAKFVPSWKPCPNYCEEQQQNTPHSPEAQEDKTFISPFFSELVLSSPMVAAARNNLTGLA 533

Query: 483 DMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISE---PIDSDTA 542
            +EDQ+NQQSIISCAS++YI+ST S YS GKGSE G+FGHP+ EK +IS     +D++  
Sbjct: 534 KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 593

Query: 543 VSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLC 602
            S+ST IDFAKPSLISSCS MSK+LSLSSFS+ S+EERDH DELK+EIDAIDLQYHQCLC
Sbjct: 594 GSLSTTIDFAKPSLISSCSEMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCLC 653

Query: 603 ELSRMREEAIESVKKRWMAKKKACAI 612
           ELSRMREEAIES KKRWM+KKKA  I
Sbjct: 654 ELSRMREEAIESAKKRWMSKKKATGI 679

BLAST of MC06g1803 vs. NCBI nr
Match: XP_038874814.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 999 bits (2582), Expect = 0.0
Identity = 516/627 (82.30%), Postives = 555/627 (88.52%), Query Frame = 0

Query: 3   LMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 62
           +MSGNSE +DRE+ EKDP+GRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL
Sbjct: 8   MMSGNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 67

Query: 63  QSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRK 122
           QSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD+ KTINMITELFTSGSLRQYRKKHRK
Sbjct: 68  QSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKRKTINMITELFTSGSLRQYRKKHRK 127

Query: 123 VDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQS 182
           VD+KAFKNWARQILRGLTYLHGHNP IIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 
Sbjct: 128 VDLKAFKNWARQILRGLTYLHGHNPAIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQP 187

Query: 183 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSG 242
           TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG
Sbjct: 188 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG 247

Query: 243 TKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQF 302
            KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  E+ KEHSSE SRLLN QF
Sbjct: 248 IKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSELSRLLNEQF 307

Query: 303 ISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEMT 362
            S NPPL+ S+P+D   +C KLSGS +SS KS   ISHFS+LELQR TENN L LKG+MT
Sbjct: 308 KSANPPLIDSHPMDTDPNCSKLSGSVASSVKSNNGISHFSTLELQRLTENNVLTLKGDMT 367

Query: 363 DHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDEL 422
           DHNT+SFHLRIAE  GKSKNIHFAFYL+SDT+LAIALEMVEQLELSNEDAT IAKLIDEL
Sbjct: 368 DHNTMSFHLRIAELYGKSKNIHFAFYLNSDTSLAIALEMVEQLELSNEDATIIAKLIDEL 427

Query: 423 ITKFVPSWKPCANIC---------SETQEDKTFIPPFFPELVLSSPMVADA--RKSLTHL 482
           I KFVPSWKPC N C         SET E+KTFIPPFF ELVLSSPMVA A  R +   L
Sbjct: 428 IAKFVPSWKPCPNYCEEQNTPNSSSETPEEKTFIPPFFSELVLSSPMVAAAAARNNPRDL 487

Query: 483 ADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISE---PIDSDT 542
             +EDQ+NQQSIISCAS++YI+ST S YS GKGSE G+FGHPE +K +IS     +D++ 
Sbjct: 488 DKVEDQENQQSIISCASVEYIYSTVSDYSMGKGSECGEFGHPECDKAYISSGTIDLDAEG 547

Query: 543 AVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCL 602
             S+ T+IDFAKPSLISSCSGMSK+LSLSSFS+ S+EERDH DELK+EIDAIDLQYHQCL
Sbjct: 548 VGSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCL 607

Query: 603 CELSRMREEAIESVKKRWMAKKKACAI 612
           CELSRMREEAIES KKRWM+KKKA  I
Sbjct: 608 CELSRMREEAIESAKKRWMSKKKATGI 634

BLAST of MC06g1803 vs. ExPASy TrEMBL
Match: A0A6J1DYE3 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111025697 PE=4 SV=1)

HSP 1 Score: 1194 bits (3088), Expect = 0.0
Identity = 611/647 (94.44%), Postives = 612/647 (94.59%), Query Frame = 0

Query: 1   MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIED 60
           MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIED
Sbjct: 1   MDLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIED 60

Query: 61  VLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKH 120
           VLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKH
Sbjct: 61  VLQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKH 120

Query: 121 RKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQ 180
           RKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQ
Sbjct: 121 RKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQ 180

Query: 181 QSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVT 240
           QSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVT
Sbjct: 181 QSTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVT 240

Query: 241 SGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNA 300
           SGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNA
Sbjct: 241 SGTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNA 300

Query: 301 QFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS-------------------------- 360
           QFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS                          
Sbjct: 301 QFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKSSNEISHFSTPELSAKSSNEISHFSTL 360

Query: 361 ---------ISHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFY 420
                    +SHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFY
Sbjct: 361 ELTAKSINEVSHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFY 420

Query: 421 LDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANICSETQEDKTFIP 480
           LDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANICSETQEDKTFIP
Sbjct: 421 LDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANICSETQEDKTFIP 480

Query: 481 PFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYG 540
           PFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYG
Sbjct: 481 PFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYG 540

Query: 541 DFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERD 600
           DFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERD
Sbjct: 541 DFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERD 600

Query: 601 HHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI 612
           HHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI
Sbjct: 601 HHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI 647

BLAST of MC06g1803 vs. ExPASy TrEMBL
Match: A0A5A7TLP6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001600 PE=4 SV=1)

HSP 1 Score: 1013 bits (2620), Expect = 0.0
Identity = 523/628 (83.28%), Postives = 559/628 (89.01%), Query Frame = 0

Query: 3   LMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 62
           +MS NSE +DRE+ EKDP+GRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL
Sbjct: 8   IMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 67

Query: 63  QSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRK 122
           QSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD++KTINMITELFTSGSLRQYRKKHRK
Sbjct: 68  QSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRK 127

Query: 123 VDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQS 182
           VD+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 
Sbjct: 128 VDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQP 187

Query: 183 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSG 242
           TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG
Sbjct: 188 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG 247

Query: 243 TKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQF 302
            KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLV E+ KEHSSE SR LN QF
Sbjct: 248 IKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQF 307

Query: 303 ISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEMT 362
            SVNPPLLGS+P+D  H C KLSGS +SS KS   ISHFS+ ELQR TENNEL LKGEMT
Sbjct: 308 KSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMT 367

Query: 363 DHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDEL 422
           DHNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDAT IAKLIDEL
Sbjct: 368 DHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDEL 427

Query: 423 ITKFVPSWKPCANIC-----------SETQEDKTFIPPFFPELVLSSPMVADARKSLTHL 482
           I KFVPSWKPC N C           SE QE+K+FI PFF ELVLSSPMV  AR +LT L
Sbjct: 428 IAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGL 487

Query: 483 ADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISE-PIDSDTAV 542
           A +EDQ+NQQSIISCAS++YI+ST S YS GKGSE G+FGHP+ EK +IS   ID D   
Sbjct: 488 AKVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEA 547

Query: 543 ---SVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQC 602
              ++ST+IDFAKPSLISSCSGMSK+LSLSSFS+ S+EERDH DELK+EIDAIDLQYHQC
Sbjct: 548 VGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQC 607

Query: 603 LCELSRMREEAIESVKKRWMAKKKACAI 612
           LCELSRMREEAIES KKRWM+KKKA  I
Sbjct: 608 LCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of MC06g1803 vs. ExPASy TrEMBL
Match: A0A1S3AUL5 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103482852 PE=4 SV=1)

HSP 1 Score: 1013 bits (2618), Expect = 0.0
Identity = 523/628 (83.28%), Postives = 558/628 (88.85%), Query Frame = 0

Query: 3   LMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 62
           +MS NSE +DRE+ EKDP+GRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL
Sbjct: 8   IMSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 67

Query: 63  QSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRK 122
           QSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD++KTINMITELFTSGSLRQYRKKHRK
Sbjct: 68  QSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRK 127

Query: 123 VDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQS 182
           VD+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 
Sbjct: 128 VDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQP 187

Query: 183 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSG 242
           TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG
Sbjct: 188 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG 247

Query: 243 TKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQF 302
            KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLV E+ KEHSSE SR LN QF
Sbjct: 248 IKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQF 307

Query: 303 ISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEMT 362
            SVNPPLLGS+P+D  H C KLSGS +SS KS   ISHFS+ ELQR TENNEL LKGEMT
Sbjct: 308 KSVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMT 367

Query: 363 DHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDEL 422
           DHNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDAT IAKLIDEL
Sbjct: 368 DHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDEL 427

Query: 423 ITKFVPSWKPCANIC-----------SETQEDKTFIPPFFPELVLSSPMVADARKSLTHL 482
           I KFVPSWKPC N C           SE QE+K+FI PFF ELVLSSPMV  AR +LT L
Sbjct: 428 IAKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGL 487

Query: 483 ADMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISE-PIDSDTAV 542
           A +EDQ+NQQSIISCAS +YI+ST S YS GKGSE G+FGHP+ EK +IS   ID D   
Sbjct: 488 AKVEDQENQQSIISCASAEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEA 547

Query: 543 ---SVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQC 602
              ++ST+IDFAKPSLISSCSGMSK+LSLSSFS+ S+EERDH DELK+EIDAIDLQYHQC
Sbjct: 548 VGSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQC 607

Query: 603 LCELSRMREEAIESVKKRWMAKKKACAI 612
           LCELSRMREEAIES KKRWM+KKKA  I
Sbjct: 608 LCELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of MC06g1803 vs. ExPASy TrEMBL
Match: A0A0A0KQP1 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5G148620 PE=4 SV=1)

HSP 1 Score: 1005 bits (2598), Expect = 0.0
Identity = 516/626 (82.43%), Postives = 556/626 (88.82%), Query Frame = 0

Query: 3   LMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 62
           +MSGNSE +DR + EKDP+GRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL
Sbjct: 54  IMSGNSEADDRGIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVL 113

Query: 63  QSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRK 122
           QSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD++KTINMITELFTSGSLRQYRKKHRK
Sbjct: 114 QSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRK 173

Query: 123 VDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQS 182
           VD+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 
Sbjct: 174 VDLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQP 233

Query: 183 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSG 242
           TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG
Sbjct: 234 TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG 293

Query: 243 TKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQF 302
            KPASLEKVLDPQVKQFIEKCLVPAS RLPASELLKDPFL  E+ K++SSE SR LN  F
Sbjct: 294 IKPASLEKVLDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSELSRSLNEHF 353

Query: 303 ISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEMT 362
            SVNPPLLGS+P++  H+C KLSGS +SS KS   ISHFS+ ELQR TENNEL LKG+MT
Sbjct: 354 KSVNPPLLGSHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGDMT 413

Query: 363 DHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDEL 422
           DHNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDAT IAKLIDEL
Sbjct: 414 DHNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDEL 473

Query: 423 ITKFVPSWKPCANICSE----------TQEDKTFIPPFFPELVLSSPMVADARKSLTHLA 482
           I KFVPSWKPC N C E           QEDKTFI PFF ELVLSSPMVA AR +LT LA
Sbjct: 474 IAKFVPSWKPCPNYCEEQQQNTPHSPEAQEDKTFISPFFSELVLSSPMVAAARNNLTGLA 533

Query: 483 DMEDQDNQQSIISCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISE---PIDSDTA 542
            +EDQ+NQQSIISCAS++YI+ST S YS GKGSE G+FGHP+ EK +IS     +D++  
Sbjct: 534 KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 593

Query: 543 VSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLC 602
            S+ST IDFAKPSLISSCS MSK+LSLSSFS+ S+EERDH DELK+EIDAIDLQYHQCLC
Sbjct: 594 GSLSTTIDFAKPSLISSCSEMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCLC 653

Query: 603 ELSRMREEAIESVKKRWMAKKKACAI 612
           ELSRMREEAIES KKRWM+KKKA  I
Sbjct: 654 ELSRMREEAIESAKKRWMSKKKATGI 679

BLAST of MC06g1803 vs. ExPASy TrEMBL
Match: A0A6J1H579 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111459693 PE=4 SV=1)

HSP 1 Score: 989 bits (2556), Expect = 0.0
Identity = 508/627 (81.02%), Postives = 549/627 (87.56%), Query Frame = 0

Query: 2   DLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDV 61
           D MSGNSE +D  + EKDPTGRYIRYDEILGKGAFKTV+KAFD+ GGIEVAWSQVDIEDV
Sbjct: 6   DFMSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIEDV 65

Query: 62  LQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHR 121
           LQSPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD  KTINMITELFTSGSLRQYRKKHR
Sbjct: 66  LQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRQYRKKHR 125

Query: 122 KVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 181
           KVD+KAFKNWAR+ILRGLTYLHG +PPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIV+QQ
Sbjct: 126 KVDLKAFKNWARKILRGLTYLHGQDPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVLQQ 185

Query: 182 STARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTS 241
            TARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTS
Sbjct: 186 PTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTS 245

Query: 242 GTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQ 301
           G  PASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFL  EN K+HSSEPS LLNAQ
Sbjct: 246 GVMPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSEPSMLLNAQ 305

Query: 302 FISVNPPLLGSNPVDIAHSCMKLSGSESSSAKS---ISHFSSLELQRFTENNELMLKGEM 361
           F SVNPPLL +NP+D+ H+C KLSGS +SS +S   ISHFS+ EL+  TENNEL LKG+M
Sbjct: 306 FESVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLKGDM 365

Query: 362 TDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDE 421
           TDHN+ISFHLRIAE  G+SKNIHFAF+LDSDTALAIALEMVEQLELSNEDAT IA+LIDE
Sbjct: 366 TDHNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLIDE 425

Query: 422 LITKFVPSWKPCANIC---------SETQEDKTFIPPFFPELVLSSPMVADARKSLTHLA 481
           LI KFVPSW+ C N C         SET+EDK F PP F E+VLSSP+V   RK+LT LA
Sbjct: 426 LIAKFVPSWQQCTNCCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTELA 485

Query: 482 DMEDQDNQQSIISCASLDYIHSTTSGYSTGKG-SEYGDFGHPEGEKVFISE---PIDSDT 541
           ++E Q+NQQ+IISCASLDY HST S YS GKG SE GDF HPE +K FIS      D D 
Sbjct: 486 EVEHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGSGTSDLDA 545

Query: 542 AVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCL 601
             SVST+ DFAKPSLISSCSGMSK+LSLS+FS+FSVEERD HDELK+EIDAID+QYHQCL
Sbjct: 546 VGSVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQHDELKMEIDAIDMQYHQCL 605

Query: 602 CELSRMREEAIESVKKRWMAKKKACAI 612
           CELSRMREEAIES KKRWM+KKK  AI
Sbjct: 606 CELSRMREEAIESAKKRWMSKKKGAAI 632

BLAST of MC06g1803 vs. TAIR 10
Match: AT5G41990.1 (with no lysine (K) kinase 8 )

HSP 1 Score: 558.9 bits (1439), Expect = 5.0e-159
Identity = 316/604 (52.32%), Postives = 401/604 (66.39%), Query Frame = 0

Query: 8   SEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQ 67
           S  E+ + AEKDP+GRYIRYD++LG+GAFKTVYKAFDEV GIEVAW+ V IEDV+Q P Q
Sbjct: 12  SSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIEDVMQMPGQ 71

Query: 68  LQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKVDMKA 127
           L+RLYSEVHLLK+LKHENIIK    WVD++NKTINMITELFTSGSLR YRKKHRKVD KA
Sbjct: 72  LERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPKA 131

Query: 128 FKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQSTARSV 187
            KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTGEVKIGDLGLA V+QQ TARSV
Sbjct: 132 IKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARSV 191

Query: 188 IGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGTKPAS 247
           IGTPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPYNEC+N AQI+KKVTS  KP S
Sbjct: 192 IGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQS 251

Query: 248 LEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQFISVNP 307
           L KV DPQV+QFIEKCL+PAS R  A EL KDPFL  +  K+ +   S   ++++  V P
Sbjct: 252 LGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSALLASSSTSSKY--VRP 311

Query: 308 PLLGSNPVDIAHSCMKLSGSESSSAKSISHFSSLELQRFTENNELMLKGEMTDHNTISFH 367
           P L   P+D+ H+         SS +      ++ELQR  EN E  L+GE +D  T S  
Sbjct: 312 PQLEHLPMDVDHN----ENKSVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVTASMV 371

Query: 368 LRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSW 427
           LRIA+  GK + +HFAFYL+SDTA AIA EMVE+L L++++   IA +ID+ I + +   
Sbjct: 372 LRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQLL--- 431

Query: 428 KPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTHLADMEDQDNQ---QSIISCA 487
                       D+T           +SP +        +   +  +D +   QS+ S  
Sbjct: 432 -----------SDRT-----SSHHNQNSPRLTHEDHEAANQQTVNSKDEEAAGQSMKSDI 491

Query: 488 SLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSC 547
           S DY       YS   G+   + G                 A S+S+ +D        SC
Sbjct: 492 SADYYFP----YSANDGNAAMEAGR---------------DAESMSSYLD--------SC 551

Query: 548 SGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWM 607
           S M      S+  + S+ + D+ ++LK E++ I+ Q++Q   +L +++E+AIE+ K++W+
Sbjct: 552 SMM------STIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKLKEDAIENAKRKWI 557

Query: 608 AKKK 609
            KK+
Sbjct: 612 TKKQ 557

BLAST of MC06g1803 vs. TAIR 10
Match: AT1G64630.1 (with no lysine (K) kinase 10 )

HSP 1 Score: 521.9 bits (1343), Expect = 6.8e-148
Identity = 304/600 (50.67%), Postives = 370/600 (61.67%), Query Frame = 0

Query: 11  EDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQR 70
           E+ +  +KDPTGRYIRY+++LG+GAFKTVYKAFDEV GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 71  LYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKVDMKAFKN 130
           LYSEVHLL SLKH+NIIK    WVDD NK+INMITELFTSGSL  YRKKHRKVD KA  N
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 131 WARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQSTARSVIGT 190
           WARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG+VKIGDLGLA VMQQ TARSVIGT
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 191 PEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGTKPASLEK 250
           PEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSG KP SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 251 VLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQFISVNPPLL 310
           V DPQVKQFIEKCL+PA  R  A ELLKD  L ++  K+ +   S   N  F    PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTLTASS--NTTFKPAMPPQC 301

Query: 311 GSNPVDIAHSCMKLSGSESSSAKSISH---FSSLELQRFTENNELMLKGEMTDHNTISFH 370
              P+D+ +     S S  SSAKS        ++E+QR  E+ E  L GE  D    S  
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 371 LRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSW 430
           LRIA   G+++ + F F L +DTA A+  EMVE+L+LS+ + T IA++IDELI K     
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMKL---- 421

Query: 431 KPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTH---LADMEDQDNQQSIISCA 490
                                            A +SL +   +   +D++  +S+ S  
Sbjct: 422 --------------------------------KANRSLPNANSVYQSKDEEAGESMKSEI 481

Query: 491 SLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSC 550
           S DY H      S+ +GS  G                           ++    S + SC
Sbjct: 482 SADYYHRV----SSNEGSRLG----------------------CCCEAVESLLSSFLDSC 521

Query: 551 SGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWM 605
           S +S   S               ++LK E++ I+ QY+Q    L RM+EEAIE  K++WM
Sbjct: 542 SMVSNKQS---------------EDLKTELNVIESQYNQSCQRLLRMKEEAIEKAKRKWM 521

BLAST of MC06g1803 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 512.3 bits (1318), Expect = 5.4e-145
Identity = 266/435 (61.15%), Postives = 322/435 (74.02%), Query Frame = 0

Query: 2   DLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDV 61
           D  S   EP D EV E DPT RYIRY E++GKGAFKTVYKAFDEV GIEVAW+QV I+DV
Sbjct: 5   DDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDV 64

Query: 62  LQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHR 121
           LQSP  L+RLYSEV LLKSLKH NII+F++ W+DD+NKT+N+ITELFTSGSLR YRKKHR
Sbjct: 65  LQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHR 124

Query: 122 KVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 181
           KV+MKA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q
Sbjct: 125 KVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQ 184

Query: 182 STARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTS 241
           + A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKN+AQI+KKV+S
Sbjct: 185 ANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSS 244

Query: 242 GTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHS--SEPSRLLN 301
           G KPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL +     ++    P  ++ 
Sbjct: 245 GIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMP 304

Query: 302 AQFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKSISHFSS------LELQRFTENNELM 361
            +    +  L+   P     S       +  S   I  FS       +E++R    N  +
Sbjct: 305 KEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSRCIEVRRAKRGNFFV 364

Query: 362 LKGEMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIA 421
           LKGE  D  ++S  LRI ++ G+ +NIHF FY + DTA  ++ EMVEQLEL++++ T IA
Sbjct: 365 LKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTFIA 424

Query: 422 KLIDELITKFVPSWK 429
           +LID L+   +P+WK
Sbjct: 425 ELIDILLVNMIPTWK 439

BLAST of MC06g1803 vs. TAIR 10
Match: AT3G18750.3 (with no lysine (K) kinase 6 )

HSP 1 Score: 512.3 bits (1318), Expect = 5.4e-145
Identity = 266/435 (61.15%), Postives = 322/435 (74.02%), Query Frame = 0

Query: 2   DLMSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDV 61
           D  S   EP D EV E DPT RYIRY E++GKGAFKTVYKAFDEV GIEVAW+QV I+DV
Sbjct: 5   DDASALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDV 64

Query: 62  LQSPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHR 121
           LQSP  L+RLYSEV LLKSLKH NII+F++ W+DD+NKT+N+ITELFTSGSLR YRKKHR
Sbjct: 65  LQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHR 124

Query: 122 KVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ 181
           KV+MKA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q
Sbjct: 125 KVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQ 184

Query: 182 STARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTS 241
           + A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKN+AQI+KKV+S
Sbjct: 185 ANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSS 244

Query: 242 GTKPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHS--SEPSRLLN 301
           G KPASL +V DP+VKQFIEKCL+PAS RL A ELL DPFL +     ++    P  ++ 
Sbjct: 245 GIKPASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMP 304

Query: 302 AQFISVNPPLLGSNPVDIAHSCMKLSGSESSSAKSISHFSS------LELQRFTENNELM 361
            +    +  L+   P     S       +  S   I  FS       +E++R    N  +
Sbjct: 305 KEGAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSRCIEVRRAKRGNFFV 364

Query: 362 LKGEMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIA 421
           LKGE  D  ++S  LRI ++ G+ +NIHF FY + DTA  ++ EMVEQLEL++++ T IA
Sbjct: 365 LKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTFIA 424

Query: 422 KLIDELITKFVPSWK 429
           +LID L+   +P+WK
Sbjct: 425 ELIDILLVNMIPTWK 439

BLAST of MC06g1803 vs. TAIR 10
Match: AT1G49160.2 (Protein kinase superfamily protein )

HSP 1 Score: 496.9 bits (1278), Expect = 2.3e-140
Identity = 286/606 (47.19%), Postives = 382/606 (63.04%), Query Frame = 0

Query: 9   EPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQL 68
           EP D EV E DPT RYIRY E++GKGA KTV+K FDEV GIEVAW+QV I+D+LQSP+ L
Sbjct: 12  EPPDPEVLEIDPTCRYIRYKEVIGKGASKTVFKGFDEVDGIEVAWNQVRIDDLLQSPDCL 71

Query: 69  QRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKVDMKAF 128
           +RLYSEV LLKSLKH+NII+F++ W+DD+NKT+N+ITELFTSGSLRQYRKKHRKV+MKA 
Sbjct: 72  ERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYRKKHRKVNMKAV 131

Query: 129 KNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQSTARSVI 188
           K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN GEVKIGDLGLA VM+Q+ A+SVI
Sbjct: 132 KCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLATVMEQANAKSVI 191

Query: 189 GTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGTKPASL 248
           GTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT EYPY EC+N+AQI+KKV+SG KPASL
Sbjct: 192 GTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYKKVSSGIKPASL 251

Query: 249 EKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHS--SEPSRLLNAQFISVN 308
            KV DP+V +FIEKCL+PAS RL A ELL D FL +     ++    P  ++  +     
Sbjct: 252 SKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPDIVMPKEGSFGE 311

Query: 309 PPLLGSNPVDIAHSCMKLSGSESSS-----AKSISHFSSLELQRFTENNELMLKGEMTDH 368
             L+   P +  +  M ++  E ++     + + S  + +E++R    N  +LKGE  D 
Sbjct: 312 RCLMSEGPPNARNRTMSMNLDEDNNLPIVISSNNSGTNCIEVRRAKRGNFFVLKGEENDE 371

Query: 369 NTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATTIAKLIDELIT 428
           N++S  LRI ++ G+ +NIHF F+ + DTA  ++ EMVEQLEL++++   IA+LID L+ 
Sbjct: 372 NSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELTDKNVKFIAELIDVLLV 431

Query: 429 KFVPSWKPCANICSETQEDKTFIPPFFPELVLSSPMVADARKSLTHLADMEDQDNQQSII 488
             +P+WK                                   ++ HL  +  Q NQ    
Sbjct: 432 NLIPNWK--------------------------------TDVAVDHL--IHPQQNQ---- 491

Query: 489 SCASLDYIHSTTSGYSTGKGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLI 548
                    S+   +  G  S+ G+          IS  + SD         D A P++ 
Sbjct: 492 ---------SSKDNHQNGASSQAGE---------SISHSLSSDYCPRSD---DEANPTVA 551

Query: 549 SSCSGMSKDLSLSSFSSFSVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKK 608
           ++      +         S+EE +  + LK E++ I+ ++ + + E++R REEA    K 
Sbjct: 552 ATTEDQEAE------KPGSLEEEEEDERLKEELEKIEERFREEMKEITRKREEATMETKN 552

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q944Q07.0e-15852.32Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=... [more]
Q6EU498.9e-15349.69Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX... [more]
Q8RXE59.5e-14750.67Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8S8Y87.6e-14461.15Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8LST23.3e-13947.19Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
XP_022159288.10.094.44probable serine/threonine-protein kinase WNK10 [Momordica charantia][more]
KAA0042657.10.083.28serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa] ... [more]
XP_008437436.10.083.28PREDICTED: serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo][more]
XP_004143946.10.082.43serine/threonine-protein kinase WNK8 isoform X1 [Cucumis sativus] >KGN49991.1 hy... [more]
XP_038874814.10.082.30serine/threonine-protein kinase WNK8-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DYE30.094.44Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A5A7TLP60.083.28Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AUL50.083.28Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KQP10.082.43Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5... [more]
A0A6J1H5790.081.02Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G41990.15.0e-15952.32with no lysine (K) kinase 8 [more]
AT1G64630.16.8e-14850.67with no lysine (K) kinase 10 [more]
AT3G18750.15.4e-14561.15with no lysine (K) kinase 6 [more]
AT3G18750.35.4e-14561.15with no lysine (K) kinase 6 [more]
AT1G49160.22.3e-14047.19Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 25..282
e-value: 1.5E-60
score: 217.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 28..282
e-value: 2.1E-51
score: 174.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 25..282
score: 41.45998
NoneNo IPR availableGENE3D3.10.20.90coord: 362..445
e-value: 5.4E-7
score: 31.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 9..106
e-value: 6.1E-36
score: 124.4
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 108..288
e-value: 7.8E-52
score: 177.4
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 29..221
e-value: 1.7E-12
score: 45.0
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 12..273
e-value: 1.4E-26
score: 91.0
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 8..607
NoneNo IPR availablePANTHERPTHR13902:SF139WNK KINASEcoord: 8..607
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 23..282
e-value: 3.80357E-170
score: 482.496
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 365..420
e-value: 3.0E-6
score: 27.2
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 149..161
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 19..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1803.1MC06g1803.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity