MC06g1761 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1761
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionTranscription initiation factor IIF subunit alpha
LocationMC06: 24890628 .. 24895818 (+)
RNA-Seq ExpressionMC06g1761
SyntenyMC06g1761
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTTTTACTCGAGGAAAAGGATTGGAGTAAGAATAAATACAGAAAAAGTTAGAAAATTTGTTTCGTAAGACAACAATTATTTACTTCGAAAAAGGGCTAAGGGTGCCTGCGGTCTCGAGACTCAGAATCATAGTATTATTTCCGGTTTCGGTCTGAAGAACTGCGATTTTTGCAATTCTCCTATCTCAGAAAGCTTACTCTCTTTCTTAGATCCTGTAACTACTGTCGATTGATGTGGATTCTGCACCAACTGGTCATAATTCTGCAAATTTACGGCCAGAGAAACTTGAAGTTTTCAATCTTGTTGGGGTTCTTCATCAATTGAGTGAGTTTTTGGCTGTTATTTGGGAACCCTAGATTTTTCAATTGGGATTCTTATGTGATTTGTGCTATTTTTCTTTTTGGCGGTTTTGTAGATTTTTGTGGCTAAATAAGGAGGTGGGAAAATGTCATTCGATCTGCTGCTGAAGCCGTCGTGCAGTGGATGCGGGTCCTCGACGGATTTGTATGGGAGCAATTGTAAGCACATGACTTTGTGCTTGACCTGCGGCAAAACTATGGCTGAGAATAAGGGCAAATGTTACGACTGTGGCGCTACTGTAACTCGCTTAATAAGAGTTCGTCTTCTACCTCGTCTTTATTAAGGAACATGGTGATTTCAATATGTTAAATTCGTACTCATGTGATTAGGATTTTTATTTACGACAGATTTAAGAATTTTCATGTCTGTCTTCTCTTTCTGAGAGCGCTGATAATATTTTAGGAATACAATGTTCGAGCAAGTGCAAGTAGCGACAAGAACTACTTCATTGGTAGGTTCATGAGTGGGCTGCCAAACTTCTCGAGGAAGAGAAATGCTGAAAATAAATGGTCTCTTTACAAGGAAGGTCTTCAAGGACGCCAGCTTTCTGATGCCTTGCGGGTACTGAGTTAACTTCTATTTTGCATTCCTTTTAAAGTTTGTATTTGGATAATTAATTGTTTCGAGAGCTTGATTCCTTTAGAAGCTGTTTCTGTTGGGGGAAAATAGGAGCAAAAGAAATTGAGGCTATCCCCCGGCAAAATGAATTGGAAATACACCTTCTTCTTTAACAAGATTGGGAGTTGCAATATTACCTTGAAACAAAGGCTTAGGAAATTTATAGATTTGGAGTCTCGTGCACTTTTTTTGCCACTTCGTGAGCTTCTGCCTCATTGGAATACAGAAATACTTGCTATTTGTATTAGGCTGCACTTTGGTGTTTCATATGGCTTACATATGAGTTGGACGTGGACCATGTAATTGTGTGTTTTGTACTTCAATATTTATATATTGCTCTGTGTTGTTAATCATAATTCAGCTTTGACCATGGAGATGTATTTGGTTGATTTCTTGTTGTATTTATCTTTTTATTGTTTCTTACTTCAAGTTAGTTGTTTTGTGGCTTATTTCTCTTCTCTTCCTTTTTCCTTTTTAAATATTATTTGCAGTGTCCTACTTGACCCTGATTAATTATTACTCCATGAACATAGGAGAAATACAAGAATAGACCCTGGCTCTTGGAAGATGAAACGGGCCAATTTCAGTTCCAAGGACATCTTGAAGGTTCACAGTCAGCAACATATTACTTGCTTGTATTGCAAGGGAAGGAATTTACTGCCATTCCTGCTGGTTCTTGGTACGAGTGAACTTTGAGTCAGTTTGCTATGAATCGAGCTGCAGTCTTTTGTTGTTTGACTTAAGTTTTCTTCATACATGGTTATTACATTTTCAATTTTCGAATGATTCTATATTTTTTGTGAGACAATGGTTCTATTGGATCCAAACACGATGTCAAATTTCAAGTCAGCCATGGTGACAAGGGGATAACTAACCTCAATTTCCAGGTACAATTTCAACAAAGTGGCACAGTATAAGCAACTTACGTTGGAGGAGGCAGAAGAGAAAATGAAGAATCGAAAGAAAACAGCTGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCTTTTGGTGAAGTCGAGAAGTTTGATGACAAAGATGCTGTTGGTGCTAGTGGTCGGGGGCGCAGGAAAACTACTGGTGATGAGAATGAAGGTAATGCCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCGGCAAGGAAGAACAGGTTGGGCCTCAATAAAAAGAGCGGCGATGATGATGATGAGGAAGGTCCTAGGGGAGGTGACCATGACATGGACGATGATGATATTGAGAAAGGTTATATTTCTTCAAATAAAAAGAAGACTTTTTCTAAAACTTTTATTTACTTATTTTAAGGTTAAGTGCTTTTATCATATCATCTCTACCTCTATGGAAGGTCATTTGCACCATTTGATTGTTTATCAGATAGTAGGCCATCATATGGGAATTAATTGCATCAATCGTAGGGTTTTATGTTGTGGATGTAAATGGGTTAGCAAATTCTTTTGTTCTTATCCAAGAGATTCAAATTTTCACTATGCATTTTCTCATATTCAAACATGTGAAAATTTTCTTTTAATTTGCATGAAGAGCATTAAGTCCACTGCCAATATTCCTTTACGTTAATGTGACTGTCTAACCTTTTAGGAGATGATTGGGAGCACGAAGAAATTTTTACTGATGATGACGAAACTACTGCTCCTGATCGTGAAGAAAGGGAGGATTTGGCCCCCGAGGTTCCGGCACCTCCAGAAATCAAGCAGGTTGTCTAGCGCTTATTTGGAAAAAAAAAAAAGCTCTCACTGATACGTTATTTGTACAATTCTACATATTTCAACAGTTGAAGTAATTGTATATGTTTTACTCCAAAAGTGTTGAAAAAGGTGGTAAGAAATGAAGCTAATGCCGGATGGGGGACGGGAAACAGGAACGGAGGAGGGAAGACTTTTATTAGTGATCATAATTTTTGTTGGAAGTAGAGTCTACAACCATCATTCACATGCACATATGGGAGTGATTAGATGAAAACAGTATATTGGAGCATTTTCCATTGGGCGTTAATTTTTCATCTGCTGATATAAGTGCAAATGTAATTATGAAACCCGTCAGATGTTTGCTATCAAAAGAGTAACTTCAGGATTCTAGAATTCTTACCAACAGGATTTACAACCTGACCTTAAAATATGGAAAGTTAATTGATTAGGAAAAGGAAAAGAGCAAGATGTACAAGGATGAGAATATATTTTTGGCTTGGAAGTTACTTATTTACTGAATGGTGGTCATGGAAGTTCTCTATTATTATTTTATTCTTGGTCTCTCGTGGAATATGTGCTGCTAGAGTCTTCAATTTTTATTATTCTTTAATTATACAATAGAACATTTCTGAAGACGTATGGGGTATGTTCTTCTCCAAGGACCTACGGGATGGCCAATTTGATTCTCAATGCGAAACATCGTCGACATTTTTGTGGTTTCACTTGGTAGTGGTGGAACTGGATCCCCCCAATATACACTTTCTCAATGTTTGATTTCCGTCGACCTTAAAAATAACCAAGAGTTCTGGTCTAACATGTTTTATTGTCTTAAACTTTTCCTGAATATATAAAGATACAGATGGTTTTTGTTCTGTAAATTCTTATCTCACTATGCAGGATGAAGACGACGAGGATGAAGAAAATGAAGGGGAGGGTGGTCTGAGCAAATCTGGAAAAGAGTTGAAGAAATTGCTTAGGCGTACTGGAGGTCTTAATGATTCAGATGGAGAGGATGACGACGACGATGACGATGTAAGCTCTTGTGATTCCTGTTAATAGTACTTAACAGCATGATCCAAACCTTTAAATAATGACCTACAAGCACTTTGTTGCCTGGAATCTGTGTGAATTGCGCTCAATAATTTGTCATGGTATCTTTATGCAGTTTGAAGAAGTGTTATTTGATTATGTGTTATGCTGCCCTTTCTCTGATAGGACTTTCGTTAGCGACAAAAAGAATACCTGATTGTATCAGTATTTATATATAATCCTCTGTTTATTGTGATACTGCCTGTATGTTTATGTAATTGTTAAGTGACTTTATACTTTTTATTTGTTCAGGTGGAGGAGGATATTGGTGGCACCCCAGTTGTGGCTTCGAAGCAGAAGGATACAGTTAAAGAAGAAGCTGCAGCCAGCAGTCCCTCAAAGCCTATACCTTCTGGATCTTCTAAGGGAACCCCGTCTTCTACGAAGGCATCCAAGGGGAAAAGAAAATTAAATGGCGACGATGTGAAACCATCTAATGTACCTCCGAAGAAAGTGAAAACCGAGACGGTATGCCATGTTTTACCATCATCTCATGGTTTAAGCTCCGAGAAATGTACTTTTTATTTGTAGTATAAGGATGAGCTGGCTAAGTCATTTTCCGTGGTAAACAAACATCTCTTATGGTATTCAGGATTCGAAGTCTACAGACGAGGGTGTGTCTACATCTAAAAGTACGGCCTCGAAAGGTGCACCGGCATCAGTCAAAACAGAATCGGCATTGCCTTCTGGACCTGTTACTGAAGAAGAAATCAGGGCTGTTTTGCGGCAAAGGACTCCTGTGACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTAAGGTCTCAAGAGGTATGTAGCGTCTTTACCGTATGTTTTACATAAACTGTTCTTTCTTTCGGTGGGTTATGTGTATGAAGACCAACTCTGCAAATCACAATGTCAATGAAACAAGTTACAGTTTCTGGTTGGGATGCTGTTTAAGACGGATGTTATATCGTTCATCTTCGGAATGAACGCTGAGAAATTTAACGGACCATGGAAATTTTCTGATGCCTTATTCATCCCATGATATGCAGGACAAGAATGCATTTGCAGAAATACTGAGGAGAATTTCAAGGATCCAAAAGACTAATGGAGGACCAAGTTATGTTGTGTTGAGAAAACCTGAAGACTAGAAGAAGATTTCAATTTATGCTTCTTTTTGATGCAGCTGGTTCATGATTCCCATCTGGTTGGTGGGTTACGGCCATCAAAATGTGTATTTATTTACTTTTTCGATACAAAGGATAAATTTGTGACCTTGAAAGAGGCATTCGCTGTTGGAATTTAAGTTGCAAGAGATTTGTCCACATTAATTAAGAAACGAAATATTCCACAGACCCTTTGTCATATTGGTTGTCATATTGATTTCCTCATTTTTTAGAACGTATAAAATAATGGAAGTTATATAAAAAAAATG

mRNA sequence

CCTTTTTACTCGAGGAAAAGGATTGGAGTAAGAATAAATACAGAAAAAGTTAGAAAATTTGTTTCGTAAGACAACAATTATTTACTTCGAAAAAGGGCTAAGGGTGCCTGCGGTCTCGAGACTCAGAATCATAGTATTATTTCCGGTTTCGGTCTGAAGAACTGCGATTTTTGCAATTCTCCTATCTCAGAAAGCTTACTCTCTTTCTTAGATCCTGTAACTACTGTCGATTGATGTGGATTCTGCACCAACTGGTCATAATTCTGCAAATTTACGGCCAGAGAAACTTGAAGTTTTCAATCTTGTTGGGGTTCTTCATCAATTGAATTTTTGTGGCTAAATAAGGAGGTGGGAAAATGTCATTCGATCTGCTGCTGAAGCCGTCGTGCAGTGGATGCGGGTCCTCGACGGATTTGTATGGGAGCAATTGTAAGCACATGACTTTGTGCTTGACCTGCGGCAAAACTATGGCTGAGAATAAGGGCAAATGTTACGACTGTGGCGCTACTGTAACTCGCTTAATAAGAGAATACAATGTTCGAGCAAGTGCAAGTAGCGACAAGAACTACTTCATTGGTAGGTTCATGAGTGGGCTGCCAAACTTCTCGAGGAAGAGAAATGCTGAAAATAAATGGTCTCTTTACAAGGAAGGTCTTCAAGGACGCCAGCTTTCTGATGCCTTGCGGGAGAAATACAAGAATAGACCCTGGCTCTTGGAAGATGAAACGGGCCAATTTCAGTTCCAAGGACATCTTGAAGGTTCACAGTCAGCAACATATTACTTGCTTGTATTGCAAGGGAAGGAATTTACTGCCATTCCTGCTGGTTCTTGGTACAATTTCAACAAAGTGGCACAGTATAAGCAACTTACGTTGGAGGAGGCAGAAGAGAAAATGAAGAATCGAAAGAAAACAGCTGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCTTTTGGTGAAGTCGAGAAGTTTGATGACAAAGATGCTGTTGGTGCTAGTGGTCGGGGGCGCAGGAAAACTACTGGTGATGAGAATGAAGGTAATGCCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCGGCAAGGAAGAACAGGTTGGGCCTCAATAAAAAGAGCGGCGATGATGATGATGAGGAAGGTCCTAGGGGAGGTGACCATGACATGGACGATGATGATATTGAGAAAGGAGATGATTGGGAGCACGAAGAAATTTTTACTGATGATGACGAAACTACTGCTCCTGATCGTGAAGAAAGGGAGGATTTGGCCCCCGAGGTTCCGGCACCTCCAGAAATCAAGCAGGATGAAGACGACGAGGATGAAGAAAATGAAGGGGAGGGTGGTCTGAGCAAATCTGGAAAAGAGTTGAAGAAATTGCTTAGGCGTACTGGAGGTCTTAATGATTCAGATGGAGAGGATGACGACGACGATGACGATGTGGAGGAGGATATTGGTGGCACCCCAGTTGTGGCTTCGAAGCAGAAGGATACAGTTAAAGAAGAAGCTGCAGCCAGCAGTCCCTCAAAGCCTATACCTTCTGGATCTTCTAAGGGAACCCCGTCTTCTACGAAGGCATCCAAGGGGAAAAGAAAATTAAATGGCGACGATGTGAAACCATCTAATGTACCTCCGAAGAAAGTGAAAACCGAGACGGATTCGAAGTCTACAGACGAGGGTGTGTCTACATCTAAAAGTACGGCCTCGAAAGGTGCACCGGCATCAGTCAAAACAGAATCGGCATTGCCTTCTGGACCTGTTACTGAAGAAGAAATCAGGGCTGTTTTGCGGCAAAGGACTCCTGTGACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTAAGGTCTCAAGAGGACAAGAATGCATTTGCAGAAATACTGAGGAGAATTTCAAGGATCCAAAAGACTAATGGAGGACCAAGTTATGTTGTGTTGAGAAAACCTGAAGACTAGAAGAAGATTTCAATTTATGCTTCTTTTTGATGCAGCTGGTTCATGATTCCCATCTGGTTGGTGGGTTACGGCCATCAAAATGTGTATTTATTTACTTTTTCGATACAAAGGATAAATTTGTGACCTTGAAAGAGGCATTCGCTGTTGGAATTTAAGTTGCAAGAGATTTGTCCACATTAATTAAGAAACGAAATATTCCACAGACCCTTTGTCATATTGGTTGTCATATTGATTTCCTCATTTTTTAGAACGTATAAAATAATGGAAGTTATATAAAAAAAATG

Coding sequence (CDS)

ATGTCATTCGATCTGCTGCTGAAGCCGTCGTGCAGTGGATGCGGGTCCTCGACGGATTTGTATGGGAGCAATTGTAAGCACATGACTTTGTGCTTGACCTGCGGCAAAACTATGGCTGAGAATAAGGGCAAATGTTACGACTGTGGCGCTACTGTAACTCGCTTAATAAGAGAATACAATGTTCGAGCAAGTGCAAGTAGCGACAAGAACTACTTCATTGGTAGGTTCATGAGTGGGCTGCCAAACTTCTCGAGGAAGAGAAATGCTGAAAATAAATGGTCTCTTTACAAGGAAGGTCTTCAAGGACGCCAGCTTTCTGATGCCTTGCGGGAGAAATACAAGAATAGACCCTGGCTCTTGGAAGATGAAACGGGCCAATTTCAGTTCCAAGGACATCTTGAAGGTTCACAGTCAGCAACATATTACTTGCTTGTATTGCAAGGGAAGGAATTTACTGCCATTCCTGCTGGTTCTTGGTACAATTTCAACAAAGTGGCACAGTATAAGCAACTTACGTTGGAGGAGGCAGAAGAGAAAATGAAGAATCGAAAGAAAACAGCTGATGGATATGAAAGATGGATGATGAAAGCTGCAAGTAATGGTCCTGCTGCTTTTGGTGAAGTCGAGAAGTTTGATGACAAAGATGCTGTTGGTGCTAGTGGTCGGGGGCGCAGGAAAACTACTGGTGATGAGAATGAAGGTAATGCCTCAGATAGGGGAGAAGAAGATGAAGAAGAAGAGGCGGCAAGGAAGAACAGGTTGGGCCTCAATAAAAAGAGCGGCGATGATGATGATGAGGAAGGTCCTAGGGGAGGTGACCATGACATGGACGATGATGATATTGAGAAAGGAGATGATTGGGAGCACGAAGAAATTTTTACTGATGATGACGAAACTACTGCTCCTGATCGTGAAGAAAGGGAGGATTTGGCCCCCGAGGTTCCGGCACCTCCAGAAATCAAGCAGGATGAAGACGACGAGGATGAAGAAAATGAAGGGGAGGGTGGTCTGAGCAAATCTGGAAAAGAGTTGAAGAAATTGCTTAGGCGTACTGGAGGTCTTAATGATTCAGATGGAGAGGATGACGACGACGATGACGATGTGGAGGAGGATATTGGTGGCACCCCAGTTGTGGCTTCGAAGCAGAAGGATACAGTTAAAGAAGAAGCTGCAGCCAGCAGTCCCTCAAAGCCTATACCTTCTGGATCTTCTAAGGGAACCCCGTCTTCTACGAAGGCATCCAAGGGGAAAAGAAAATTAAATGGCGACGATGTGAAACCATCTAATGTACCTCCGAAGAAAGTGAAAACCGAGACGGATTCGAAGTCTACAGACGAGGGTGTGTCTACATCTAAAAGTACGGCCTCGAAAGGTGCACCGGCATCAGTCAAAACAGAATCGGCATTGCCTTCTGGACCTGTTACTGAAGAAGAAATCAGGGCTGTTTTGCGGCAAAGGACTCCTGTGACTACACAGGATCTTGTTGCAAAATTCAAAGCAAGACTAAGGTCTCAAGAGGACAAGAATGCATTTGCAGAAATACTGAGGAGAATTTCAAGGATCCAAAAGACTAATGGAGGACCAAGTTATGTTGTGTTGAGAAAACCTGAAGACTAG

Protein sequence

MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYNVRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLLEDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRGEEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGEDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKLNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Homology
BLAST of MC06g1761 vs. ExPASy Swiss-Prot
Match: Q9SU25 (Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=3702 GN=RAP74 PE=1 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 8.3e-187
Identity = 376/547 (68.74%), Postives = 439/547 (80.26%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS  L L  SC GCGS +DLYGS+C+HMTLCL CG+TMA+NK KC++CG  VTRLIREYN
Sbjct: 1   MSNCLQLNTSCVGCGSQSDLYGSSCRHMTLCLKCGRTMAQNKSKCHECGTVVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRA+A +DKNYFIGRF++GLPNF  K+ +ENKWSL K+  QGRQ +DA REK KN+PW+L
Sbjct: 61  VRAAAPTDKNYFIGRFVTGLPNF--KKGSENKWSLRKDIPQGRQFTDAQREKLKNKPWIL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQ+QGHLEGSQSATYYLLV+Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQYQGHLEGSQSATYYLLVMQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAV-GASGRGRRKTT-GDENEGNASD 240
           KNR+KTADGY+RWMMKAA+NGPA FGEV+   +     G  GRGR+K++ GDE EGN SD
Sbjct: 181 KNRRKTADGYQRWMMKAANNGPALFGEVDNEKESGGTSGGGGRGRKKSSGGDEEEGNVSD 240

Query: 241 RGEEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDE 300
           RG+EDEEEEA+RK+RLGLN+KS DDDDEEGPRGGD DMDDDDIEKGDDWEHEEIFTDDDE
Sbjct: 241 RGDEDEEEEASRKSRLGLNRKSNDDDDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDE 300

Query: 301 TTAPDREERED-LAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDS 360
               D EERED LAPE+PAPPEIKQDEDDE+ E E EGGLSKSGKELKKLL +  GL++S
Sbjct: 301 AVGNDPEEREDLLAPEIPAPPEIKQDEDDEENEEE-EGGLSKSGKELKKLLGKANGLDES 360

Query: 361 DGEDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGK 420
           D +DDDD DD EE   GT V  SKQK+  KEE   ++P+KP PSG  +GTP + K SKGK
Sbjct: 361 DEDDDDDSDDEEETNYGT-VTNSKQKEAAKEEPVDNAPAKPAPSGPPRGTPPA-KPSKGK 420

Query: 421 RKLN-GDDVKPSNVPPKKVKTETDSKST---DEGVSTSKST----ASKGAPASV-KTESA 480
           RKLN GD  KPS+   KKVKTE D KS+   +   + SKS     A K  PAS   + S+
Sbjct: 421 RKLNDGDSKKPSSSVQKKVKTENDPKSSLKEERANTVSKSNTPTKAVKAEPASAPASSSS 480

Query: 481 LPSGPVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGP 536
             +GPVTE+EIRAVL ++  VTTQDLV++FKARL+++EDKNAFA ILR+IS+IQK  G  
Sbjct: 481 AATGPVTEDEIRAVLMEKKQVTTQDLVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQ 540

BLAST of MC06g1761 vs. ExPASy Swiss-Prot
Match: Q54WV4 (General transcription factor IIF subunit 1 OS=Dictyostelium discoideum OX=44689 GN=gtf2f1 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 4.9e-14
Identity = 117/422 (27.73%), Postives = 189/422 (44.79%), Query Frame = 0

Query: 114 KNRPWLLEDETGQFQFQGHLEGSQ-SATYYLLVLQGK-EFTAIPAGSWYNFNKVAQYKQL 173
           K  PW LED  G   +QG++EG+Q S+ Y+L + Q      A+P   WYNF    +++ L
Sbjct: 146 KTIPWKLEDSEGNNTYQGNVEGNQASSNYFLFMFQSDGSIKAVPCNDWYNFRPKKEFQSL 205

Query: 174 TLEEAEEKMKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDE 233
           T EEAE+ MK + +  D +   + K      +  G  E    K          R    D 
Sbjct: 206 TTEEAEDFMKKKNQEWDVFTSRLQKKTDPSGSGSGGSESSGKKSIEELEEAEHRNRNEDP 265

Query: 234 NEGNASDRGEEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEE 293
           N    ++  EE ++++A  + R   ++ +G+D D         D DDD    GD      
Sbjct: 266 NRYKTTN--EEKKKKKAPARRREREDEGNGEDGDAPDFESKFDDDDDDTYMDGDG----- 325

Query: 294 IFTDDDETTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRT 353
             T +DE    + EE ED+        E+K+    + ++N+ E  L+    + K     T
Sbjct: 326 -LTGEDENQIEEDEEEEDV-DLTEGGLEMKKMIKKQQQQNDSEDDLA-DDDDKKDNNGGT 385

Query: 354 GGLNDSDGEDDDDDDDVEEDIGGTPVVASKQ------KDTVKEEAAASSPSKPIPSGSSK 413
           GG +D D ++DD+D D ++D+   P V  K         +VK+E      SK       K
Sbjct: 386 GGGDDEDDDEDDEDPD-QDDLSNLPKVFGKSNADGTTNSSVKKEDDGGKDSKSSKKSKKK 445

Query: 414 GTPSSTKASKGKRKLNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKT 473
              SS K+ + K K N  D    N   KK+K E  S    +  S S+           + 
Sbjct: 446 DKDSSPKSKEKKSKKNKSDSSVDNRESKKIKKEPSSPQAVQPNSPSQQQQQ-------QQ 505

Query: 474 ESALPSG-PVTEEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKT 527
           ++  P+  P TEE I+ VL++   V + DL+  FK  L++ ++K  F  ++  ++R+ + 
Sbjct: 506 QNIDPNDPPFTEEYIKIVLQKSKKVKSLDLINIFKGPLKNPDNKPIFLAMVHNVARVVEE 549

BLAST of MC06g1761 vs. ExPASy Swiss-Prot
Match: Q05913 (General transcription factor IIF subunit 1 OS=Drosophila melanogaster OX=7227 GN=TfIIFalpha PE=1 SV=3)

HSP 1 Score: 52.4 bits (124), Expect = 1.9e-05
Identity = 131/479 (27.35%), Postives = 217/479 (45.30%), Query Frame = 0

Query: 116 RPWLLE--DETGQFQFQGHLEG--SQSATYYLLV-LQGKEFTAIPAGSWYNFNKVAQYKQ 175
           +PW+L+   +TG+ +F+G  EG   ++A +Y+          A P   WYNF  + +YK 
Sbjct: 123 QPWILKVGGKTGK-KFKGIREGGVGENAAFYVFTHAPDGAIEAYPLTEWYNFQPIQRYKS 182

Query: 176 LTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDA---VGASGRGRRKT 235
           L+ EEAE++   RKK  + Y   M++    G     E E+ D ++A     A+ + +   
Sbjct: 183 LSAEEAEQEFGRRKKVMN-YFSLMLRKRLRG----DEEEEQDPEEAKLIKAATKKSKELK 242

Query: 236 TGDENEGNASDRGEEDEEEEAARKNRLGLNKKSGDDDD----EEGPRGGD-----HDMDD 295
             D +E   S+   + E+EE  +K      K+  D DD     +G +G D      D+DD
Sbjct: 243 ITDMDEWIDSEDESDSEDEEDKKK------KEQEDSDDGKAKGKGKKGADKKKKKRDVDD 302

Query: 296 DDIEKGDDWEHEEIFTDDDETTA---PDREERED---------------LAPEVPAPPEI 355
           +  E+ DD + E    D D +++   PD E + D               L  +     E 
Sbjct: 303 EAFEESDDGDEEGREMDYDTSSSEDEPDPEAKVDKDMKGVAEEDALRKLLTSDEEEDDEK 362

Query: 356 KQDEDDEDE-------ENEGEGGLSKSGKELKKL----LRRTGGLNDSDGEDDDDDDDVE 415
           K DE D+++       +++G+  +SK  K+ K        ++ G  DS  +   D  D E
Sbjct: 363 KSDESDKEDADGEKKKKDKGKDEVSKDKKKKKPTKDDKKGKSNGSGDSSTDFSSDSTDSE 422

Query: 416 EDIGGTP-----VVASKQKDTVKEEAAASSPSKPIPSGSS-----KGTPS-STKASKGKR 475
           +D+   P     VV  K K+  KE+ +A+S SK I S S+       TP+ ST AS  KR
Sbjct: 423 DDLSNGPPKKKVVVKDKDKEKEKEKESAAS-SKVIASSSNANKSRSATPTLSTDAS--KR 482

Query: 476 KLNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEE 535
           K+N       ++P     ++T +  T       K+  S   P S         G +TEE 
Sbjct: 483 KMN-------SLPSDLTASDTSNSPTSTPAKRPKNEISTSLPTSFSGGKVEDYG-ITEEA 542

BLAST of MC06g1761 vs. NCBI nr
Match: XP_022159266.1 (transcription initiation factor IIF subunit alpha [Momordica charantia])

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETT 300
           EEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETT
Sbjct: 241 EEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETT 300

Query: 301 APDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGE 360
           APDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGE
Sbjct: 301 APDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGE 360

Query: 361 DDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKL 420
           DDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKL
Sbjct: 361 DDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKL 420

Query: 421 NGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEIR 480
           NGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEIR
Sbjct: 421 NGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEIR 480

Query: 481 AVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           AVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 AVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538

BLAST of MC06g1761 vs. NCBI nr
Match: XP_004152016.1 (transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >XP_011651639.1 transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >KGN58337.1 hypothetical protein Csa_017587 [Cucumis sativus])

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 500/539 (92.76%), Postives = 521/539 (96.66%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLK SCSGCGS+T+LYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKQSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRASAS+DKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL
Sbjct: 61  VRASASNDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK+ VG SGRGRRKTTGDENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDET 300
           EEDEEEEAARKNRLGL+KK G DDDDEEGPRGGDHD+DDDDIEKGDDWEHEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360
           TAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360

Query: 361 EDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRK 420
           EDDDDDDDV+ED GGTPV+ SKQKDTVKEEAAA+SPSKP PSGS+KGTPS+ K +KGKRK
Sbjct: 361 EDDDDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGKRK 420

Query: 421 LNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEI 480
            NG+DVKPS+ PPKK+KTET+SK+TDEGV TSKSTASKGAPASVKTE +  SGPVTEEEI
Sbjct: 421 -NGEDVKPSSAPPKKMKTETESKATDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEEI 480

Query: 481 RAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           RAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 RAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538

BLAST of MC06g1761 vs. NCBI nr
Match: XP_038888042.1 (transcription initiation factor IIF subunit alpha isoform X1 [Benincasa hispida])

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 500/539 (92.76%), Postives = 519/539 (96.29%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDL+LKPSCSGCGS+T+LYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLMLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFS+KRNAENKWSLYK+GLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKDGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK+ VG SGRGRRK TGDENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKEGVGTSGRGRRKPTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDET 300
           EEDEEEEA RKNRLGLNKK G DDDDEEGPRGGDHD+DDDDIEKGDDWEHEEIFTDDDET
Sbjct: 241 EEDEEEEAQRKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360
           TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360

Query: 361 EDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRK 420
           EDDDDDDDV+ED GGTPV+ SKQKDTVKEEAAA+SPSKP PSGS+KGTPS+ K +KGKRK
Sbjct: 361 EDDDDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGKRK 420

Query: 421 LNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEI 480
            NG+DVKPS+ PPKK+KTET+SKSTDE VSTSKSTASKGAPA VKTE A  SGPVTEEEI
Sbjct: 421 -NGEDVKPSSAPPKKMKTETESKSTDE-VSTSKSTASKGAPALVKTEPASHSGPVTEEEI 480

Query: 481 RAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           RAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 RAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 537

BLAST of MC06g1761 vs. NCBI nr
Match: XP_016900419.1 (PREDICTED: LOW QUALITY PROTEIN: transcription initiation factor IIF subunit alpha [Cucumis melo])

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 497/539 (92.21%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLKPSCSGCGS+T+LYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAE KM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEXKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK+ VG SGRGRRKTTGDENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDET 300
           EEDEEEEAARKNRLGL+KK G DDDDEEGPRGGDHD+DDDDIEKGDDWEHEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360
           TAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360

Query: 361 EDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRK 420
           EDD+DDDDV+ED GGTPV+ SKQKDTVKEEAAA+SPSKP PSGS+KGTPS+ K +KGKRK
Sbjct: 361 EDDEDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGKRK 420

Query: 421 LNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEI 480
            NG+DVKPS+ PPKK+KTET+ K TDEGV TSKSTASKGAPASVKTE +  SGPVTEEEI
Sbjct: 421 -NGEDVKPSSAPPKKMKTETELKPTDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEEI 480

Query: 481 RAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           RAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQK NGGPSYVVLRKPED
Sbjct: 481 RAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKINGGPSYVVLRKPED 538

BLAST of MC06g1761 vs. NCBI nr
Match: XP_022931161.1 (transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 924 bits (2387), Expect = 0.0
Identity = 495/540 (91.67%), Postives = 516/540 (95.56%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS DLLLKPSCSGCGS+T+LYGSNCKHMTLCL CGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKD+VGA+GRGRRK T DENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDET 300
           EEDEEEEAARKNRLGLNKK G DDDDEEGPRGG+HD+DDDDIEKGDDWEHEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360
           TAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360

Query: 361 ED-DDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKR 420
           E+ DDDDDD EE+ GGTPV  SKQKD VKEEAAA+SPSKP PSGS+KGTPS+ K +KGKR
Sbjct: 361 EEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGKR 420

Query: 421 KLNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEE 480
           K NG+DVKPS+ PPKK+KTET+SKSTDEGVSTS+STASKGA ASVKTE A  SGPVTEEE
Sbjct: 421 K-NGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 480

Query: 481 IRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           IRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 539

BLAST of MC06g1761 vs. ExPASy TrEMBL
Match: A0A6J1E3E1 (Transcription initiation factor IIF subunit alpha OS=Momordica charantia OX=3673 GN=LOC111025673 PE=3 SV=1)

HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETT 300
           EEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETT
Sbjct: 241 EEDEEEEAARKNRLGLNKKSGDDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETT 300

Query: 301 APDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGE 360
           APDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGE
Sbjct: 301 APDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGE 360

Query: 361 DDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKL 420
           DDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKL
Sbjct: 361 DDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKL 420

Query: 421 NGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEIR 480
           NGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEIR
Sbjct: 421 NGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEIR 480

Query: 481 AVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           AVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 AVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538

BLAST of MC06g1761 vs. ExPASy TrEMBL
Match: A0A0A0LCA6 (Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_3G624050 PE=3 SV=1)

HSP 1 Score: 941 bits (2431), Expect = 0.0
Identity = 500/539 (92.76%), Postives = 521/539 (96.66%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLK SCSGCGS+T+LYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKQSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRASAS+DKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL
Sbjct: 61  VRASASNDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK+ VG SGRGRRKTTGDENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDET 300
           EEDEEEEAARKNRLGL+KK G DDDDEEGPRGGDHD+DDDDIEKGDDWEHEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360
           TAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360

Query: 361 EDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRK 420
           EDDDDDDDV+ED GGTPV+ SKQKDTVKEEAAA+SPSKP PSGS+KGTPS+ K +KGKRK
Sbjct: 361 EDDDDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGKRK 420

Query: 421 LNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEI 480
            NG+DVKPS+ PPKK+KTET+SK+TDEGV TSKSTASKGAPASVKTE +  SGPVTEEEI
Sbjct: 421 -NGEDVKPSSAPPKKMKTETESKATDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEEI 480

Query: 481 RAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           RAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 RAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538

BLAST of MC06g1761 vs. ExPASy TrEMBL
Match: A0A1S4DWR2 (Transcription initiation factor IIF subunit alpha OS=Cucumis melo OX=3656 GN=LOC103489800 PE=3 SV=1)

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 497/539 (92.21%), Postives = 518/539 (96.10%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MSFDLLLKPSCSGCGS+T+LYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSFDLLLKPSCSGCGSTTELYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRASASSDKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASASSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAE KM
Sbjct: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEXKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDK+ VG SGRGRRKTTGDENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKETVGTSGRGRRKTTGDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDET 300
           EEDEEEEAARKNRLGL+KK G DDDDEEGPRGGDHD+DDDDIEKGDDWEHEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLSKKGGGDDDDEEGPRGGDHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360
           TAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360

Query: 361 EDDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRK 420
           EDD+DDDDV+ED GGTPV+ SKQKDTVKEEAAA+SPSKP PSGS+KGTPS+ K +KGKRK
Sbjct: 361 EDDEDDDDVDEDTGGTPVLPSKQKDTVKEEAAANSPSKPTPSGSAKGTPSTAKTAKGKRK 420

Query: 421 LNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEEI 480
            NG+DVKPS+ PPKK+KTET+ K TDEGV TSKSTASKGAPASVKTE +  SGPVTEEEI
Sbjct: 421 -NGEDVKPSSAPPKKMKTETELKPTDEGVPTSKSTASKGAPASVKTEPSSHSGPVTEEEI 480

Query: 481 RAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           RAVLRQRTPVTTQDLVAKFKARLR+ EDKNAFAEILRRISRIQK NGGPSYVVLRKPED
Sbjct: 481 RAVLRQRTPVTTQDLVAKFKARLRTPEDKNAFAEILRRISRIQKINGGPSYVVLRKPED 538

BLAST of MC06g1761 vs. ExPASy TrEMBL
Match: A0A6J1ESX1 (Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111437424 PE=3 SV=1)

HSP 1 Score: 924 bits (2387), Expect = 0.0
Identity = 495/540 (91.67%), Postives = 516/540 (95.56%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS DLLLKPSCSGCGS+T+LYGSNCKHMTLCL CGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLRCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKD+VGA+GRGRRK T DENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSVGATGRGRRKPTSDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDET 300
           EEDEEEEAARKNRLGLNKK G DDDDEEGPRGG+HD+DDDDIEKGDDWEHEEIFTDDDET
Sbjct: 241 EEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGNHDLDDDDIEKGDDWEHEEIFTDDDET 300

Query: 301 TAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360
           TAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG
Sbjct: 301 TAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDG 360

Query: 361 ED-DDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKR 420
           E+ DDDDDD EE+ GGTPV  SKQKD VKEEAAA+SPSKP PSGS+KGTPS+ K +KGKR
Sbjct: 361 EEEDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTPSTAKTAKGKR 420

Query: 421 KLNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVTEEE 480
           K NG+DVKPS+ PPKK+KTET+SKSTDEGVSTS+STASKGA ASVKTE A  SGPVTEEE
Sbjct: 421 K-NGEDVKPSSAPPKKMKTETESKSTDEGVSTSRSTASKGAAASVKTEPASASGPVTEEE 480

Query: 481 IRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 538
           IRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLRKPED
Sbjct: 481 IRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRKPED 539

BLAST of MC06g1761 vs. ExPASy TrEMBL
Match: A0A6J1K1N5 (Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111491607 PE=3 SV=1)

HSP 1 Score: 914 bits (2363), Expect = 0.0
Identity = 492/543 (90.61%), Postives = 515/543 (94.84%), Query Frame = 0

Query: 1   MSFDLLLKPSCSGCGSSTDLYGSNCKHMTLCLTCGKTMAENKGKCYDCGATVTRLIREYN 60
           MS DLLLKPSCSGCGS+T+LYGSNCKHMTLCL+CGKTMAENKGKCYDCGATVTRLIREYN
Sbjct: 1   MSLDLLLKPSCSGCGSTTELYGSNCKHMTLCLSCGKTMAENKGKCYDCGATVTRLIREYN 60

Query: 61  VRASASSDKNYFIGRFMSGLPNFSRKRNAENKWSLYKEGLQGRQLSDALREKYKNRPWLL 120
           VRAS+SSDKNYFIGRFMSGLPNFS+KRNAENKWSLYKEGLQGRQL+DALREKYKNRPWLL
Sbjct: 61  VRASSSSDKNYFIGRFMSGLPNFSKKRNAENKWSLYKEGLQGRQLTDALREKYKNRPWLL 120

Query: 121 EDETGQFQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180
           EDETGQ QFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM
Sbjct: 121 EDETGQSQFQGHLEGSQSATYYLLVLQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKM 180

Query: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDAVGASGRGRRKTTGDENEGNASDRG 240
           KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKD++GA+GRGRRK T DENEGN SDRG
Sbjct: 181 KNRKKTADGYERWMMKAASNGPAAFGEVEKFDDKDSIGATGRGRRKPTSDENEGNVSDRG 240

Query: 241 EEDEEEEAARKNRLGLNKKSG-DDDDEEGPRGGDHDMDDDDIEK-GDDWEHEEIFTDDDE 300
           EEDEEEEAARKNRLGLNKK G DDDDEEGPRGGDHD+DDDDIE  GDDWEHEEIFTDDDE
Sbjct: 241 EEDEEEEAARKNRLGLNKKGGGDDDDEEGPRGGDHDLDDDDIESIGDDWEHEEIFTDDDE 300

Query: 301 TTAPDREEREDLAPEVPAPPEIKQDEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSD 360
           TTAPDREEREDLAPEVPAPPEIKQDE+DEDEENEGEGGLSKSGKELKKLLRR+GGLNDSD
Sbjct: 301 TTAPDREEREDLAPEVPAPPEIKQDEEDEDEENEGEGGLSKSGKELKKLLRRSGGLNDSD 360

Query: 361 GE---DDDDDDDVEEDIGGTPVVASKQKDTVKEEAAASSPSKPIPSGSSKGTPSSTKASK 420
           GE   DDDDDDD EE+ GGTPV  SKQKD VKEEAAA+SPSKP PSGS+KGT S+ K +K
Sbjct: 361 GEEEEDDDDDDDAEEESGGTPVAPSKQKDAVKEEAAANSPSKPAPSGSAKGTSSTAKTAK 420

Query: 421 GKRKLNGDDVKPSNVPPKKVKTETDSKSTDEGVSTSKSTASKGAPASVKTESALPSGPVT 480
           GKRK NG+DVKPS+ PPKK+KTET+SKSTDEG+STS+STASKGA ASVKTE A  SGPVT
Sbjct: 421 GKRK-NGEDVKPSSAPPKKMKTETESKSTDEGMSTSRSTASKGAAASVKTEPASASGPVT 480

Query: 481 EEEIRAVLRQRTPVTTQDLVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRK 538
           EEEIRAVLRQRTPVTTQDLVAKFKARLRS EDKNAFAEILRRISRIQKTNGGPSYVVLRK
Sbjct: 481 EEEIRAVLRQRTPVTTQDLVAKFKARLRSHEDKNAFAEILRRISRIQKTNGGPSYVVLRK 540

BLAST of MC06g1761 vs. TAIR 10
Match: AT4G12610.1 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 434.5 bits (1116), Expect = 1.2e-121
Identity = 270/402 (67.16%), Postives = 315/402 (78.36%), Query Frame = 0

Query: 146 LQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAF 205
           +Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKMKNR+KTADGY+RWMMKAA+NGPA F
Sbjct: 1   MQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRRKTADGYQRWMMKAANNGPALF 60

Query: 206 GEVEKFDDKDAV-GASGRGRRKTT-GDENEGNASDRGEEDEEEEAARKNRLGLNKKSGDD 265
           GEV+   +     G  GRGR+K++ GDE EGN SDRG+EDEEEEA+RK+RLGLN+KS DD
Sbjct: 61  GEVDNEKESGGTSGGGGRGRKKSSGGDEEEGNVSDRGDEDEEEEASRKSRLGLNRKSNDD 120

Query: 266 DDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETTAPDREERED-LAPEVPAPPEIKQ 325
           DDEEGPRGGD DMDDDDIEKGDDWEHEEIFTDDDE    D EERED LAPE+PAPPEIKQ
Sbjct: 121 DDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDEAVGNDPEEREDLLAPEIPAPPEIKQ 180

Query: 326 DEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGEDDDDDDDVEEDIGGTPVVASKQ 385
           DEDDE+ E E EGGLSKSGKELKKLL +  GL++SD +DDDD DD EE   GT V  SKQ
Sbjct: 181 DEDDEENEEE-EGGLSKSGKELKKLLGKANGLDESDEDDDDDSDDEEETNYGT-VTNSKQ 240

Query: 386 KDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKLN-GDDVKPSNVPPKKVKTETDS 445
           K+  KEE   ++P+KP PSG  +GTP + K SKGKRKLN GD  KPS+   KKVKTE D 
Sbjct: 241 KEAAKEEPVDNAPAKPAPSGPPRGTPPA-KPSKGKRKLNDGDSKKPSSSVQKKVKTENDP 300

Query: 446 KST---DEGVSTSKST----ASKGAPASV-KTESALPSGPVTEEEIRAVLRQRTPVTTQD 505
           KS+   +   + SKS     A K  PAS   + S+  +GPVTE+EIRAVL ++  VTTQD
Sbjct: 301 KSSLKEERANTVSKSNTPTKAVKAEPASAPASSSSAATGPVTEDEIRAVLMEKKQVTTQD 360

Query: 506 LVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRK 536
           LV++FKARL+++EDKNAFA ILR+IS+IQK  G  ++VVLR+
Sbjct: 361 LVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVLRE 399

BLAST of MC06g1761 vs. TAIR 10
Match: AT4G12610.2 (transcription activators;DNA binding;RNA polymerase II transcription factors;catalytics;transcription initiation factors )

HSP 1 Score: 434.5 bits (1116), Expect = 1.2e-121
Identity = 270/402 (67.16%), Postives = 315/402 (78.36%), Query Frame = 0

Query: 146 LQGKEFTAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRKKTADGYERWMMKAASNGPAAF 205
           +Q KEF AIPAGSWYNFNKVAQYKQLTLEEAEEKMKNR+KTADGY+RWMMKAA+NGPA F
Sbjct: 1   MQNKEFVAIPAGSWYNFNKVAQYKQLTLEEAEEKMKNRRKTADGYQRWMMKAANNGPALF 60

Query: 206 GEVEKFDDKDAV-GASGRGRRKTT-GDENEGNASDRGEEDEEEEAARKNRLGLNKKSGDD 265
           GEV+   +     G  GRGR+K++ GDE EGN SDRG+EDEEEEA+RK+RLGLN+KS DD
Sbjct: 61  GEVDNEKESGGTSGGGGRGRKKSSGGDEEEGNVSDRGDEDEEEEASRKSRLGLNRKSNDD 120

Query: 266 DDEEGPRGGDHDMDDDDIEKGDDWEHEEIFTDDDETTAPDREERED-LAPEVPAPPEIKQ 325
           DDEEGPRGGD DMDDDDIEKGDDWEHEEIFTDDDE    D EERED LAPE+PAPPEIKQ
Sbjct: 121 DDEEGPRGGDLDMDDDDIEKGDDWEHEEIFTDDDEAVGNDPEEREDLLAPEIPAPPEIKQ 180

Query: 326 DEDDEDEENEGEGGLSKSGKELKKLLRRTGGLNDSDGEDDDDDDDVEEDIGGTPVVASKQ 385
           DEDDE+ E E EGGLSKSGKELKKLL +  GL++SD +DDDD DD EE   GT V  SKQ
Sbjct: 181 DEDDEENEEE-EGGLSKSGKELKKLLGKANGLDESDEDDDDDSDDEEETNYGT-VTNSKQ 240

Query: 386 KDTVKEEAAASSPSKPIPSGSSKGTPSSTKASKGKRKLN-GDDVKPSNVPPKKVKTETDS 445
           K+  KEE   ++P+KP PSG  +GTP + K SKGKRKLN GD  KPS+   KKVKTE D 
Sbjct: 241 KEAAKEEPVDNAPAKPAPSGPPRGTPPA-KPSKGKRKLNDGDSKKPSSSVQKKVKTENDP 300

Query: 446 KST---DEGVSTSKST----ASKGAPASV-KTESALPSGPVTEEEIRAVLRQRTPVTTQD 505
           KS+   +   + SKS     A K  PAS   + S+  +GPVTE+EIRAVL ++  VTTQD
Sbjct: 301 KSSLKEERANTVSKSNTPTKAVKAEPASAPASSSSAATGPVTEDEIRAVLMEKKQVTTQD 360

Query: 506 LVAKFKARLRSQEDKNAFAEILRRISRIQKTNGGPSYVVLRK 536
           LV++FKARL+++EDKNAFA ILR+IS+IQK  G  ++VVLR+
Sbjct: 361 LVSRFKARLKTKEDKNAFANILRKISKIQKNAGSQNFVVLRE 399

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SU258.3e-18768.74Transcription initiation factor IIF subunit alpha OS=Arabidopsis thaliana OX=370... [more]
Q54WV44.9e-1427.73General transcription factor IIF subunit 1 OS=Dictyostelium discoideum OX=44689 ... [more]
Q059131.9e-0527.35General transcription factor IIF subunit 1 OS=Drosophila melanogaster OX=7227 GN... [more]
Match NameE-valueIdentityDescription
XP_022159266.10.0100.00transcription initiation factor IIF subunit alpha [Momordica charantia][more]
XP_004152016.10.092.76transcription initiation factor IIF subunit alpha isoform X1 [Cucumis sativus] >... [more]
XP_038888042.10.092.76transcription initiation factor IIF subunit alpha isoform X1 [Benincasa hispida][more]
XP_016900419.10.092.21PREDICTED: LOW QUALITY PROTEIN: transcription initiation factor IIF subunit alph... [more]
XP_022931161.10.091.67transcription initiation factor IIF subunit alpha-like isoform X1 [Cucurbita mos... [more]
Match NameE-valueIdentityDescription
A0A6J1E3E10.0100.00Transcription initiation factor IIF subunit alpha OS=Momordica charantia OX=3673... [more]
A0A0A0LCA60.092.76Transcription initiation factor IIF subunit alpha OS=Cucumis sativus OX=3659 GN=... [more]
A0A1S4DWR20.092.21Transcription initiation factor IIF subunit alpha OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1ESX10.091.67Transcription initiation factor IIF subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1K1N50.090.61Transcription initiation factor IIF subunit alpha OS=Cucurbita maxima OX=3661 GN... [more]
Match NameE-valueIdentityDescription
AT4G12610.11.2e-12167.16transcription activators;DNA binding;RNA polymerase II transcription factors;cat... [more]
AT4G12610.21.2e-12167.16transcription activators;DNA binding;RNA polymerase II transcription factors;cat... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 453..535
e-value: 8.0E-20
score: 72.4
IPR008851Transcription initiation factor IIF, alpha subunitPFAMPF05793TFIIF_alphacoord: 48..534
e-value: 3.0E-145
score: 484.8
IPR008851Transcription initiation factor IIF, alpha subunitPANTHERPTHR13011TFIIF-ALPHAcoord: 1..534
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 334..356
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 446..461
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 244..267
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..303
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 206..476
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 396..415
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 416..445
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 357..372
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 207..235
NoneNo IPR availablePANTHERPTHR13011:SF3BNAANNG16720D PROTEINcoord: 1..534
IPR011039Transcription Factor IIF, Rap30/Rap74, interactionSUPERFAMILY50916Rap30/74 interaction domainscoord: 53..183
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 471..534

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1761.1MC06g1761.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006413 translational initiation
cellular_component GO:0005674 transcription factor TFIIF complex
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0016251 RNA polymerase II general transcription initiation factor activity
molecular_function GO:0001096 TFIIF-class transcription factor complex binding
molecular_function GO:0003743 translation initiation factor activity