MC06g1753 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1753
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPantoate--beta-alanine ligase
LocationMC06: 24825213 .. 24828067 (-)
RNA-Seq ExpressionMC06g1753
SyntenyMC06g1753
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATTGTTTCGAAGCTTTATCGAGCGCGGTGTTCAAAAAGCCATTCGCGATGGTTCGTGGTTCGTGTTGGGTTTCGCTTTCAGTTTCCATTTTTGTTGCTCGCGGTTCTCTCGAAAGTCGAAAGTACATAGAATTTCGAATTTCGTCCACCGGTTCGAATCACTGTGAAATTGCAGTTCCAGTCTCAATCTCCAATGGCGGGGAAGGAGAAGCCGAAGATCATAATAGACAAGAACCAGATGAGAACCTGGTGTAGGGCCATGAGATCCCAAGGCAAAACCATCGCCCTCGTGCCCACCATGGGGTATCTCCACGACGGCCATCTCTCGCTCATCGAAGAGGCCCACAAGCACACCCACCTCGTCGTGGTCTCAATCTACGTAAACCCTAGTCAATTTGCCCCTTCCGAGGATCTCTCCACCTACCCTTCAGATTTCGAGGGCGATATTCGGAAGCTCATGGCTGTTCCTGGAGGAATCCATGTTGTTTTCCATCCACATAATCTCTACGACTATGGAGTAGACGCAGCTGTGGAATTTGGCGGAAATGGCGGCGGTTCTGATGGTAGGGCGGCGGTTTCTTCCCTGGAGGCGTCTGGTTCAGGGCATGAGACATGGGTGAGAGTGGAGCGCTTGGAGAAGGGGATGTGTGGGAGAAGTAGGCCTGTTTTCTTCAGAGGAGTTGCCACCATTGTTGCCAAGTTGTTTAATATTGTGGAGCCTGATGTCGCAGTGTTTGGGAAGAAGGATTATCAGCAATGGCGGATTATTCGGCGGATGGTGAGTCTTTACTTTTGTTTGTTTCTTTATGGGATAGATGAAGAGGAATGGCTTTGATTTTTCTGTCTGTTTAGAGCTCAGAAGTCATTGCTTGTCTTCTGAAGTTGATTGCTTTAGTGAAAGTTTTATAAGGAAAAACTTCTTCGAATATTGAACTTACTTCAAATGGATAATGATCAATACCATTAGATGAACTGGTAGTTAAATTCTCACCAGTATGTTGGAAAAGCACTTCGAGTTGTTGATTCTGGCCTAGAGCCAATAGTTCATGATCTAGAGGATTTTCCTGTTTGGTTGCAATATAGCCCACAGGGTATGACTTGAGTTCTTGTCCCTTGGAGTTCATTTAGTTCTGATTTTGATAGTCTAAAATATGGGTGGACACTATTTACAAGGTTCTTGGATTATATATGTTTAGAACTCCGTAGAACATATATAACACATCCTGTTTCACTTCGTTTCTTGGATTATATTAATATATGCTTCTCTGAACCTTATCATCTTTAGTCTTAGACTCCGACCAGTTTATTGGATTTGCCAGTTGCTGTCATTTGATTAAGAAATGCACTTGTTTGGTGGTGATAATACTTCTCACTCAACTTTCTTGTAGGTTAGAGACCTTGATTTTTCTATAAAAGTCGTGGGGTCTGAAATCGTCCGTGATGCTGATGGTCTTGCATTGAGTTCTCGCAATGTGCACCTCTCACCTGAAGAAAGACAAAAGGTTCTCTTCGATTAACTAAGTAGTATAGTCTGTTTAGTTGTTGTGATTTACTTTTATCAGACTGTTATTTGTTGGTTAGTCTTAGTTAGTTTTGTAATTATTCTTTAAATAGTGTGTTATGCTTTCTCAGATTTCTTAATGAGAAGAGCCAATTTCATTTTATGAAGTATTATTTCATCTCCATCACAGTTCTTTCTGTCTACCTCTTTTCAAACTATAGTTTCTGCAGAGTGCAAAGAATTTCGCCTTTATCATTCATGATGGATTGATTACTTAGATTCAGAAACTAATTTCCCTTTCTCATCTCATCTTTTGTCAATAGGCATTGTCTATAAACAGGTCATTGTCAAAAGCAAAATCTGCAGCAGAAAGAGGTGAACGTAATTGCAAAAAATTGCAGAATATGATGGTCGATGAAATACTAGAAGCCGGTGGACGACTTGATTATGCTGAGGTGAGCCCTAGATGATTTAATTTTAAATTAGGAAGTCTTTCCGACTTGAGACTTGACTTTGGGAGCCTATATTAAGCTTTGATGTTCTGTTTGTGTAGCCTTCAGCTTAATTAAGAGATATTGTGGAGTTGTAGCTTGTCCATCCCATTGTTTTATTGAACAACCACTTTTCCTGATAAGGATGAAAATGAAAGGAGTTGTTTTAAAAAAAATAGCCATTTGCATTTGTTTCCAAAAGCTACTTTTAAAAACTAGAAAATGGGGCTGCTCAAAAGTTTATTAGGTATACGAAATCATGGTTCACTTCTAGTTCTAGTCCATTATTTTGGAAGGTTATTTTTTTTCTTTTCTTTTTTTTTTTTTATAATTGGGATTCGGAGCTTTGCTCCCCTATACCCCAGGACACCCGTGCCCACCCCAAGACCCGGACCCAGGAGACATCAAGATTTTCTAGTATTAAGCTTCCTCTAGTATTAAGCTTATTGGAAGGTTAACTTCGATTCTTCAAAACCAGAATGGTAGAAAACAACTTTTATTTACATAATGTTATCCAAAACACGGAAACACACGTTTCTTGAATTTTTGTTTTGGAAAGCATAAATTTTTCTTTCAAGAACGCTTCGATTAGATATTTAATTAGTAATTTTAACAAATTATTTTGCTGGTGTTATGGGTTGATTGATCGAGAGTATGTAAAAATTGTCATGTGGTTTTTTGTTTGGTGGCAGATTGTGGAACAAGAGAGTTTAGAGGTGGTGGAGGAGATCAAGAGCCCAGTTGTATTCTTGATTGCTGCCTGGTTTGGGAAAGTCAGGCTCATAGACAATATGGAAATAAAGTTATGAACATTTGAACACTTATTTCTTTTTCCTTCTGCTCACGTTTGCTCATGGGGGG

mRNA sequence

AAAATTGTTTCGAAGCTTTATCGAGCGCGGTGTTCAAAAAGCCATTCGCGATGGTTCGTGGTTCGTGTTGGGTTTCGCTTTCAGTTTCCATTTTTGTTGCTCGCGGTTCTCTCGAAAGTCGAAAGTACATAGAATTTCGAATTTCGTCCACCGGTTCGAATCACTGTGAAATTGCAGTTCCAGTCTCAATCTCCAATGGCGGGGAAGGAGAAGCCGAAGATCATAATAGACAAGAACCAGATGAGAACCTGGTGTAGGGCCATGAGATCCCAAGGCAAAACCATCGCCCTCGTGCCCACCATGGGGTATCTCCACGACGGCCATCTCTCGCTCATCGAAGAGGCCCACAAGCACACCCACCTCGTCGTGGTCTCAATCTACGTAAACCCTAGTCAATTTGCCCCTTCCGAGGATCTCTCCACCTACCCTTCAGATTTCGAGGGCGATATTCGGAAGCTCATGGCTGTTCCTGGAGGAATCCATGTTGTTTTCCATCCACATAATCTCTACGACTATGGAGTAGACGCAGCTGTGGAATTTGGCGGAAATGGCGGCGGTTCTGATGGTAGGGCGGCGGTTTCTTCCCTGGAGGCGTCTGGTTCAGGGCATGAGACATGGGTGAGAGTGGAGCGCTTGGAGAAGGGGATGTGTGGGAGAAGTAGGCCTGTTTTCTTCAGAGGAGTTGCCACCATTGTTGCCAAGTTGTTTAATATTGTGGAGCCTGATGTCGCAGTGTTTGGGAAGAAGGATTATCAGCAATGGCGGATTATTCGGCGGATGGTTAGAGACCTTGATTTTTCTATAAAAGTCGTGGGGTCTGAAATCGTCCGTGATGCTGATGGTCTTGCATTGAGTTCTCGCAATGTGCACCTCTCACCTGAAGAAAGACAAAAGGCATTGTCTATAAACAGGTCATTGTCAAAAGCAAAATCTGCAGCAGAAAGAGGTGAACGTAATTGCAAAAAATTGCAGAATATGATGGTCGATGAAATACTAGAAGCCGGTGGACGACTTGATTATGCTGAGATTGTGGAACAAGAGAGTTTAGAGGTGGTGGAGGAGATCAAGAGCCCAGTTGTATTCTTGATTGCTGCCTGGTTTGGGAAAGTCAGGCTCATAGACAATATGGAAATAAAGTTATGAACATTTGAACACTTATTTCTTTTTCCTTCTGCTCACGTTTGCTCATGGGGGG

Coding sequence (CDS)

ATGGCGGGGAAGGAGAAGCCGAAGATCATAATAGACAAGAACCAGATGAGAACCTGGTGTAGGGCCATGAGATCCCAAGGCAAAACCATCGCCCTCGTGCCCACCATGGGGTATCTCCACGACGGCCATCTCTCGCTCATCGAAGAGGCCCACAAGCACACCCACCTCGTCGTGGTCTCAATCTACGTAAACCCTAGTCAATTTGCCCCTTCCGAGGATCTCTCCACCTACCCTTCAGATTTCGAGGGCGATATTCGGAAGCTCATGGCTGTTCCTGGAGGAATCCATGTTGTTTTCCATCCACATAATCTCTACGACTATGGAGTAGACGCAGCTGTGGAATTTGGCGGAAATGGCGGCGGTTCTGATGGTAGGGCGGCGGTTTCTTCCCTGGAGGCGTCTGGTTCAGGGCATGAGACATGGGTGAGAGTGGAGCGCTTGGAGAAGGGGATGTGTGGGAGAAGTAGGCCTGTTTTCTTCAGAGGAGTTGCCACCATTGTTGCCAAGTTGTTTAATATTGTGGAGCCTGATGTCGCAGTGTTTGGGAAGAAGGATTATCAGCAATGGCGGATTATTCGGCGGATGGTTAGAGACCTTGATTTTTCTATAAAAGTCGTGGGGTCTGAAATCGTCCGTGATGCTGATGGTCTTGCATTGAGTTCTCGCAATGTGCACCTCTCACCTGAAGAAAGACAAAAGGCATTGTCTATAAACAGGTCATTGTCAAAAGCAAAATCTGCAGCAGAAAGAGGTGAACGTAATTGCAAAAAATTGCAGAATATGATGGTCGATGAAATACTAGAAGCCGGTGGACGACTTGATTATGCTGAGATTGTGGAACAAGAGAGTTTAGAGGTGGTGGAGGAGATCAAGAGCCCAGTTGTATTCTTGATTGCTGCCTGGTTTGGGAAAGTCAGGCTCATAGACAATATGGAAATAAAGTTATGA

Protein sequence

MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVSIYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGGGSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAVFGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRSLSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAAWFGKVRLIDNMEIKL
Homology
BLAST of MC06g1753 vs. ExPASy Swiss-Prot
Match: O24035 (Pantoate--beta-alanine ligase OS=Lotus japonicus OX=34305 GN=PANC PE=1 SV=3)

HSP 1 Score: 444.9 bits (1143), Expect = 7.6e-124
Identity = 219/313 (69.97%), Postives = 263/313 (84.03%), Query Frame = 0

Query: 7   PKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVSIYVNPS 66
           P +I DK++MR W R+MRSQGK IALVPTMG+LH+GHLSL+ +AH H  LV VSIYVNP 
Sbjct: 3   PMVISDKDEMRKWSRSMRSQGKLIALVPTMGFLHEGHLSLVRDAHNHADLVAVSIYVNPG 62

Query: 67  QFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDY---GVDAAVEFGGNGGGSD 126
           QF+P+EDLS YPSDF+GD++KLM+VPGG+ VVFHPHNLYDY   G DA  E GG+G    
Sbjct: 63  QFSPTEDLSAYPSDFQGDLQKLMSVPGGVDVVFHPHNLYDYGGDGGDAVAECGGDG---- 122

Query: 127 GRAAVSSLE-ASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAVFG 186
               VS ++  SG GHETWVR E+LEK +CG+SRPVFFRGVATIV KLFNIVEPDVAVFG
Sbjct: 123 ---VVSCVDRRSGFGHETWVRAEKLEKPLCGKSRPVFFRGVATIVTKLFNIVEPDVAVFG 182

Query: 187 KKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRSLS 246
           KKDYQQW+II+RMVRDLDFSIKV+GSE++R+ DGLA+SSRNV+LSPEER+KA+SIN+SL 
Sbjct: 183 KKDYQQWKIIQRMVRDLDFSIKVIGSEVIREKDGLAMSSRNVYLSPEEREKAVSINKSLF 242

Query: 247 KAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAAWF 306
           +AKSAAE G+ +C+KL N++V  I EAGGR+DYAEIV+Q +LE VE IK PVVF ++AWF
Sbjct: 243 RAKSAAEDGQIHCEKLINLVVQSITEAGGRIDYAEIVDQNNLEKVEWIKGPVVFCVSAWF 302

Query: 307 GKVRLIDNMEIKL 316
           GK RLIDN+EI L
Sbjct: 303 GKARLIDNIEINL 308

BLAST of MC06g1753 vs. ExPASy Swiss-Prot
Match: Q9FKB3 (Pantoate--beta-alanine ligase OS=Arabidopsis thaliana OX=3702 GN=At5g48840 PE=1 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 1.2e-113
Identity = 203/310 (65.48%), Postives = 251/310 (80.97%), Query Frame = 0

Query: 6   KPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVSIYVNP 65
           +P++I DK+ MR W RAMRSQGKTI LVPTMGYLH+GHLSL+ ++   T + VVSIYVNP
Sbjct: 2   EPEVIRDKDSMRKWSRAMRSQGKTIGLVPTMGYLHEGHLSLVRQSLALTDVTVVSIYVNP 61

Query: 66  SQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGGGSDGR 125
            QF+P+EDLSTYPSDF GD+ KL A+ GG  VVF+P NLYDYG +       N GG +G 
Sbjct: 62  GQFSPTEDLSTYPSDFSGDLTKLAALSGGKVVVFNPKNLYDYGGETKKI---NDGGGNGG 121

Query: 126 AAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAVFGKKD 185
             VS +E  G GHETW+RVERLEKG CG+SRPVFFRGVATIV KLFNIVEPDVA+FGKKD
Sbjct: 122 RVVSCVEEGGLGHETWIRVERLEKGFCGKSRPVFFRGVATIVTKLFNIVEPDVALFGKKD 181

Query: 186 YQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRSLSKAK 245
           YQQWRII+RMVRDL+F I++VGS+I R+ DGLA+SSRNV LS EERQ+ALSI+RSL+ AK
Sbjct: 182 YQQWRIIQRMVRDLNFGIEIVGSDIAREKDGLAMSSRNVRLSDEERQRALSISRSLAMAK 241

Query: 246 SAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAAWFGKV 305
           ++   G+ NC +L++M++ +++ + GR+DY EIV+QE+LE VEEIKS VV  +AAWFG V
Sbjct: 242 ASVAEGKTNCAELKDMIIQQVVGSAGRVDYVEIVDQETLEGVEEIKSGVVICVAAWFGTV 301

Query: 306 RLIDNMEIKL 316
           RLIDN+EI +
Sbjct: 302 RLIDNIEINV 308

BLAST of MC06g1753 vs. ExPASy Swiss-Prot
Match: O24210 (Pantoate--beta-alanine ligase OS=Oryza sativa subsp. japonica OX=39947 GN=PANC PE=2 SV=2)

HSP 1 Score: 391.3 bits (1004), Expect = 1.0e-107
Identity = 203/317 (64.04%), Postives = 244/317 (76.97%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTH----L 60
           MA   +P++I DK  MR W R  R++GKT+A+VPTMGYLH GHLSLI  A          
Sbjct: 1   MAAPREPEVIRDKAAMRAWSRRRRAEGKTVAVVPTMGYLHQGHLSLISAAAAAASADPVA 60

Query: 61  VVVSIYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFG 120
           +VV+IYVNPSQFAPSEDL+TYPSDF GD+RKL A  G +  VF+P +LY       V   
Sbjct: 61  IVVTIYVNPSQFAPSEDLATYPSDFAGDLRKL-ASTGVVDAVFNPPDLY-------VRGA 120

Query: 121 GNGGGSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEP 180
           G  G + G A     EA+G GHETWVRVERLEKGMCG SRPVFFRGVATIV+KLFNI+EP
Sbjct: 121 GRRGAASGGAISCLEEAAGDGHETWVRVERLEKGMCGASRPVFFRGVATIVSKLFNIIEP 180

Query: 181 DVAVFGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALS 240
           DVAVFGKKDYQQWR+I RMVRDLDF+I+++GSEIVR+ADGLA+SSRNVHLS EER+KALS
Sbjct: 181 DVAVFGKKDYQQWRVICRMVRDLDFAIEIIGSEIVREADGLAMSSRNVHLSREEREKALS 240

Query: 241 INRSLSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVF 300
           I+RSL  A++ A +G  +CK+++N +V  + E GG++DY EIVEQESL  VE+I  PVV 
Sbjct: 241 ISRSLVDARTGALKGNTDCKQIKNKIVQTLTETGGQVDYVEIVEQESLVPVEQIDGPVVI 300

Query: 301 LIAAWFGKVRLIDNMEI 314
            +AAWFGKVRLIDN+EI
Sbjct: 301 CVAAWFGKVRLIDNIEI 309

BLAST of MC06g1753 vs. ExPASy Swiss-Prot
Match: Q2IFU3 (Pantothenate synthetase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) OX=290397 GN=panC PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-66
Identity = 145/310 (46.77%), Postives = 189/310 (60.97%), Query Frame = 0

Query: 7   PKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTH------LVVVS 66
           P++I D    +  C A R  G  IALV TMGYLH+GHLSL+ EA +         L V +
Sbjct: 4   PELITDPAAWQARCTAAREAGTRIALVTTMGYLHEGHLSLMREARRRADAGGRRGLAVGT 63

Query: 67  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 126
           I+VNP+QF P+EDL+ YP D EGD+ K  A   G+  V  P        D A+ F     
Sbjct: 64  IFVNPTQFGPTEDLARYPRDLEGDLAKCAA--AGLDAVLAPS-------DPALMF----- 123

Query: 127 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 186
                           GHETWV VER  +G+ G SRP  FRGVAT+VAKLFN+  P VA+
Sbjct: 124 --------------APGHETWVTVERASQGLDGASRPGHFRGVATVVAKLFNLTRPHVAL 183

Query: 187 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 246
           FG+KD+QQ  +IR MVRDL F I++VG  IVR+ DGLALSSRN +LSP+ER++AL+++ +
Sbjct: 184 FGEKDWQQLAVIRAMVRDLAFGIEIVGMPIVREPDGLALSSRNAYLSPDERRRALALSGA 243

Query: 247 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 306
           L++A+ A  RGER+   L+      +  AGGR+DY E+V  E+L  V   +   V L+AA
Sbjct: 244 LAEAREATARGERDAAALRAGARARLEAAGGRVDYVELVHPETLAPVARAEPGTVLLLAA 285

Query: 307 WFGKVRLIDN 311
            FG  RLIDN
Sbjct: 304 SFGTTRLIDN 285

BLAST of MC06g1753 vs. ExPASy Swiss-Prot
Match: B1L843 (Pantothenate synthetase OS=Thermotoga sp. (strain RQ2) OX=126740 GN=panC PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.7e-65
Identity = 139/304 (45.72%), Postives = 188/304 (61.84%), Query Frame = 0

Query: 8   KIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVSIYVNPSQ 67
           KII    +M+ +   MR + KTI  VPTMGYLH+GHLSL+  A     +VVVSI+VNP+Q
Sbjct: 2   KIIETIEEMKKFSEEMREKKKTIGFVPTMGYLHEGHLSLVRRARDENDVVVVSIFVNPTQ 61

Query: 68  FAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGGGSDGRAA 127
           F P+ED   YP DFE D RKL+     +  +FHP                          
Sbjct: 62  FGPNEDYERYPRDFERD-RKLLE-KENVDCIFHP-------------------------- 121

Query: 128 VSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAVFGKKDYQ 187
            S  E       T+V   +L K +CGRSRP  FRGV T+V KLFNIV+P  A FG+KD Q
Sbjct: 122 -SVEEMYPPDFSTYVEETKLSKNLCGRSRPGHFRGVCTVVTKLFNIVKPHRAYFGQKDAQ 181

Query: 188 QWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRSLSKAKSA 247
           Q+R++RRMVRDL+  ++++   IVR+ DGLA+SSRNV+LSPEERQ+ALS+ +SL  A++ 
Sbjct: 182 QFRVLRRMVRDLNMDVEMIECPIVREPDGLAMSSRNVYLSPEERQQALSLYQSLKIAENL 241

Query: 248 AERGERNCKKLQNMMVDEILEAGG-RLDYAEIVEQESLEVVEEIKSPVVFLIAAWFGKVR 307
              GER+ +K++  M+  +      ++DY EIV++E+LE VE+I   V+  +AAW G  R
Sbjct: 242 YLNGERDAEKIKEEMIKHLSRFDKVKIDYVEIVDEETLEPVEKIDRKVIVAVAAWVGNAR 276

Query: 308 LIDN 311
           LIDN
Sbjct: 302 LIDN 276

BLAST of MC06g1753 vs. NCBI nr
Match: XP_022156035.1 (pantoate--beta-alanine ligase [Momordica charantia])

HSP 1 Score: 619 bits (1597), Expect = 1.66e-223
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS
Sbjct: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG
Sbjct: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
           GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV
Sbjct: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS
Sbjct: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA
Sbjct: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDNMEIKL
Sbjct: 301 WFGKVRLIDNMEIKL 315

BLAST of MC06g1753 vs. NCBI nr
Match: XP_022931128.1 (pantoate--beta-alanine ligase-like [Cucurbita moschata])

HSP 1 Score: 543 bits (1398), Expect = 3.52e-193
Identity = 272/315 (86.35%), Postives = 294/315 (93.33%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKEKP II DKNQMRTW R+MRSQGKTI+LVPTMG+LHDGHLSL++EAHKH  LVVVS
Sbjct: 1   MAGKEKPMIITDKNQMRTWTRSMRSQGKTISLVPTMGFLHDGHLSLVQEAHKHAQLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNP+QFAPSEDLSTYPSDF+GDIRKLMAVPGGI VVFHPHNLYDYGV++ ++ G NGG
Sbjct: 61  IYVNPAQFAPSEDLSTYPSDFDGDIRKLMAVPGGIDVVFHPHNLYDYGVESDLDRGANGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
           GS GRAA S LE SGS HETWVRVERLEKGMCGRSRPVFFRGVATIV KLFNIVEPDVAV
Sbjct: 121 GSGGRAADSCLEESGSEHETWVRVERLEKGMCGRSRPVFFRGVATIVTKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWR+IRRMVRDLDFSI +VGSEI+RDADGLA+SSRNV LSPEERQKALSINRS
Sbjct: 181 FGKKDYQQWRVIRRMVRDLDFSINIVGSEIMRDADGLAMSSRNVRLSPEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGE NCK+L+N++V+EI EAGGRLDYAEIVEQESLEVVEEIKSPVV L+AA
Sbjct: 241 LSKAKSAAERGELNCKRLKNLVVEEIEEAGGRLDYAEIVEQESLEVVEEIKSPVVMLVAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDNMEI +
Sbjct: 301 WFGKVRLIDNMEISI 315

BLAST of MC06g1753 vs. NCBI nr
Match: KAG6606322.1 (Pantoate--beta-alanine ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 541 bits (1395), Expect = 1.01e-192
Identity = 271/315 (86.03%), Postives = 294/315 (93.33%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKEKPKII DKNQMRTW R+MRSQGKTIALVPTMG+LHDGHLSL++EAHK   LVVVS
Sbjct: 1   MAGKEKPKIITDKNQMRTWTRSMRSQGKTIALVPTMGFLHDGHLSLVQEAHKRAQLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNP+QFAPSEDLSTYPSDF+GDIRKLMAVPGGI VVFHPHNLYDYGV++ ++ G NGG
Sbjct: 61  IYVNPAQFAPSEDLSTYPSDFDGDIRKLMAVPGGIDVVFHPHNLYDYGVESDLDRGANGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
           GS GRAA S LE SGS HETWVRVERLEKGMCGRSRPVFFRGVATIV KLFNIVEPDVAV
Sbjct: 121 GSGGRAADSCLEESGSEHETWVRVERLEKGMCGRSRPVFFRGVATIVTKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWR+IRRMVRDLDFSI +VGSEI+RDADGLA+SSRNV L+PEERQKALSINRS
Sbjct: 181 FGKKDYQQWRVIRRMVRDLDFSINIVGSEIMRDADGLAMSSRNVRLTPEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGE NCK+L+N++V+E+ EAGGRLDYAEIVEQESLEVVEEIKSPVV L+AA
Sbjct: 241 LSKAKSAAERGELNCKRLKNLVVEEVEEAGGRLDYAEIVEQESLEVVEEIKSPVVMLVAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDNMEI +
Sbjct: 301 WFGKVRLIDNMEISI 315

BLAST of MC06g1753 vs. NCBI nr
Match: XP_023533404.1 (pantoate--beta-alanine ligase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535 bits (1377), Expect = 5.57e-190
Identity = 268/315 (85.08%), Postives = 290/315 (92.06%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKEKP II DKN MRTW R+MRSQGKTIA VPTMG+LHDGHLSL++EAHKH  LVVVS
Sbjct: 1   MAGKEKPMIITDKNPMRTWTRSMRSQGKTIAFVPTMGFLHDGHLSLVQEAHKHAQLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNP+QFAPSEDLSTYPSDF+GDIRKLMAVPGGI VVFHPHNLYDYGV++ ++ G NGG
Sbjct: 61  IYVNPAQFAPSEDLSTYPSDFDGDIRKLMAVPGGIDVVFHPHNLYDYGVESDLDRGANGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
           GS GRA  S LE SGS HETWVRVERLEKGMCGRSRPV FRGVATIV KLFNIVEPDVAV
Sbjct: 121 GSGGRATDSCLEESGSEHETWVRVERLEKGMCGRSRPVVFRGVATIVTKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWR+IRRMVRDLDFSI +VGSEI+RDADGLA+SSRNV L+PEERQKALSINRS
Sbjct: 181 FGKKDYQQWRVIRRMVRDLDFSINIVGSEIMRDADGLAMSSRNVRLTPEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGE NCK+L+N++V+EI EAGGRLDYAEIVEQESLEVVEEIKSPVV L+AA
Sbjct: 241 LSKAKSAAERGELNCKRLKNLVVEEIEEAGGRLDYAEIVEQESLEVVEEIKSPVVMLVAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDNMEI +
Sbjct: 301 WFGKVRLIDNMEISI 315

BLAST of MC06g1753 vs. NCBI nr
Match: XP_038886857.1 (pantoate--beta-alanine ligase isoform X1 [Benincasa hispida])

HSP 1 Score: 532 bits (1371), Expect = 4.10e-189
Identity = 274/316 (86.71%), Postives = 290/316 (91.77%), Query Frame = 0

Query: 1   MAGKEK-PKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVV 60
           MAGKEK PKII DKNQMRTW RAMR+QGKTIALVPTMG+LHDGHLSLI+EAHKHT LVVV
Sbjct: 1   MAGKEKQPKIITDKNQMRTWTRAMRTQGKTIALVPTMGFLHDGHLSLIQEAHKHTQLVVV 60

Query: 61  SIYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNG 120
           SIYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGI VVFHPHNLYDYGV++A +  GNG
Sbjct: 61  SIYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIDVVFHPHNLYDYGVESARDCAGNG 120

Query: 121 GGSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVA 180
           G     AAVS LE SGSGHETWVRVERLEKGMCG SRPVFFRGVAT+V KLFNIVEPDVA
Sbjct: 121 G----MAAVSCLEESGSGHETWVRVERLEKGMCGSSRPVFFRGVATVVTKLFNIVEPDVA 180

Query: 181 VFGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINR 240
           VFGKKDYQQWRIIRRMVRDLDFSI +VG EIVRDADGLA+SSRNV LSPEER KALSINR
Sbjct: 181 VFGKKDYQQWRIIRRMVRDLDFSINIVGFEIVRDADGLAMSSRNVRLSPEERLKALSINR 240

Query: 241 SLSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIA 300
           SLSKAKSAAERGE NCK+L+N++VDE+ EAGG LDYAEIVEQESLEVVEEIKSPVV LIA
Sbjct: 241 SLSKAKSAAERGELNCKRLKNLVVDEVREAGGELDYAEIVEQESLEVVEEIKSPVVILIA 300

Query: 301 AWFGKVRLIDNMEIKL 315
           AWFG+VRLIDNMEI +
Sbjct: 301 AWFGEVRLIDNMEINI 312

BLAST of MC06g1753 vs. ExPASy TrEMBL
Match: A0A6J1DP64 (Pantoate--beta-alanine ligase OS=Momordica charantia OX=3673 GN=LOC111023004 PE=3 SV=1)

HSP 1 Score: 619 bits (1597), Expect = 8.03e-224
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS
Sbjct: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG
Sbjct: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
           GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV
Sbjct: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS
Sbjct: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA
Sbjct: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDNMEIKL
Sbjct: 301 WFGKVRLIDNMEIKL 315

BLAST of MC06g1753 vs. ExPASy TrEMBL
Match: A0A6J1EXI4 (Pantoate--beta-alanine ligase OS=Cucurbita moschata OX=3662 GN=LOC111437399 PE=3 SV=1)

HSP 1 Score: 543 bits (1398), Expect = 1.71e-193
Identity = 272/315 (86.35%), Postives = 294/315 (93.33%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKEKP II DKNQMRTW R+MRSQGKTI+LVPTMG+LHDGHLSL++EAHKH  LVVVS
Sbjct: 1   MAGKEKPMIITDKNQMRTWTRSMRSQGKTISLVPTMGFLHDGHLSLVQEAHKHAQLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNP+QFAPSEDLSTYPSDF+GDIRKLMAVPGGI VVFHPHNLYDYGV++ ++ G NGG
Sbjct: 61  IYVNPAQFAPSEDLSTYPSDFDGDIRKLMAVPGGIDVVFHPHNLYDYGVESDLDRGANGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
           GS GRAA S LE SGS HETWVRVERLEKGMCGRSRPVFFRGVATIV KLFNIVEPDVAV
Sbjct: 121 GSGGRAADSCLEESGSEHETWVRVERLEKGMCGRSRPVFFRGVATIVTKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWR+IRRMVRDLDFSI +VGSEI+RDADGLA+SSRNV LSPEERQKALSINRS
Sbjct: 181 FGKKDYQQWRVIRRMVRDLDFSINIVGSEIMRDADGLAMSSRNVRLSPEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGE NCK+L+N++V+EI EAGGRLDYAEIVEQESLEVVEEIKSPVV L+AA
Sbjct: 241 LSKAKSAAERGELNCKRLKNLVVEEIEEAGGRLDYAEIVEQESLEVVEEIKSPVVMLVAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDNMEI +
Sbjct: 301 WFGKVRLIDNMEISI 315

BLAST of MC06g1753 vs. ExPASy TrEMBL
Match: A0A6J1K6E0 (Pantoate--beta-alanine ligase OS=Cucurbita maxima OX=3661 GN=LOC111491569 PE=3 SV=1)

HSP 1 Score: 531 bits (1368), Expect = 6.34e-189
Identity = 265/315 (84.13%), Postives = 290/315 (92.06%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKEKPKII DKNQMRTW R+MRSQGKTIALVPTMG+LHDGHLSL++EA KH  LVVVS
Sbjct: 1   MAGKEKPKIITDKNQMRTWTRSMRSQGKTIALVPTMGFLHDGHLSLVQEAQKHAQLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNP+QFAPSEDLSTYPSDF+GDIRKL AVPGGI VVFHPHNLYDYG+++ ++ G NGG
Sbjct: 61  IYVNPAQFAPSEDLSTYPSDFDGDIRKLAAVPGGIDVVFHPHNLYDYGLESDLDRGANGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
           G  GRAA S LE SGS HETWVRVERLEKGMCGRSRPVFFRGVATIV KLFN+VEPDVAV
Sbjct: 121 GFGGRAADSCLEESGSEHETWVRVERLEKGMCGRSRPVFFRGVATIVTKLFNVVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWR+IRRMVRDLDFSI +VGSEI+RD DGLA+SSRNV LSPEERQKALSINRS
Sbjct: 181 FGKKDYQQWRVIRRMVRDLDFSINIVGSEIMRDTDGLAMSSRNVRLSPEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSK KSAAERGE +CK+L+N++V+EI EAGGRLDYAEIVEQESLEVVEEIKSPVV L+AA
Sbjct: 241 LSKTKSAAERGELSCKRLKNLVVEEIEEAGGRLDYAEIVEQESLEVVEEIKSPVVMLVAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRL+DNMEI +
Sbjct: 301 WFGKVRLLDNMEISI 315

BLAST of MC06g1753 vs. ExPASy TrEMBL
Match: A0A6J1HU15 (Pantoate--beta-alanine ligase OS=Cucurbita maxima OX=3661 GN=LOC111466858 PE=3 SV=1)

HSP 1 Score: 523 bits (1347), Expect = 9.31e-186
Identity = 270/315 (85.71%), Postives = 287/315 (91.11%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKE  KII DKNQMRTW RAMRSQGKTIALVPTMG+LHDGHLSLIEEAHKHTHLVVVS
Sbjct: 1   MAGKENLKIITDKNQMRTWTRAMRSQGKTIALVPTMGFLHDGHLSLIEEAHKHTHLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNPSQFAPSEDLSTYPSDF+GDIRKLM VPGG+  VFHP+NLYDYGV++A    GNGG
Sbjct: 61  IYVNPSQFAPSEDLSTYPSDFDGDIRKLMVVPGGVDAVFHPYNLYDYGVESARC--GNGG 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
            S+G A VS LE SGSGHETWVRVERL+KGMCG  RPVFFRGVATIVAKLFNIVEPDVAV
Sbjct: 121 VSNGTAVVSYLEESGSGHETWVRVERLQKGMCGIRRPVFFRGVATIVAKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWRIIRRMVRDLDFSI +VGSEI+RDADGLA+SSRNV LS EERQKALSINRS
Sbjct: 181 FGKKDYQQWRIIRRMVRDLDFSINIVGSEIMRDADGLAMSSRNVGLSLEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGE NCK L++++VDEI EAGG LDY EIVEQESLEVVEEIKSPVV LIAA
Sbjct: 241 LSKAKSAAERGELNCKSLKSLVVDEIREAGGELDYVEIVEQESLEVVEEIKSPVVILIAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDN+EI +
Sbjct: 301 WFGKVRLIDNIEISI 313

BLAST of MC06g1753 vs. ExPASy TrEMBL
Match: A0A6J1HLJ5 (Pantoate--beta-alanine ligase OS=Cucurbita moschata OX=3662 GN=LOC111464073 PE=3 SV=1)

HSP 1 Score: 520 bits (1339), Expect = 1.54e-184
Identity = 269/315 (85.40%), Postives = 286/315 (90.79%), Query Frame = 0

Query: 1   MAGKEKPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVS 60
           MAGKE  KII DKNQMRTW RAMRSQGKTIALVPTMG+LHDGHLSLIEEAHKHTHLVVVS
Sbjct: 1   MAGKENLKIITDKNQMRTWTRAMRSQGKTIALVPTMGFLHDGHLSLIEEAHKHTHLVVVS 60

Query: 61  IYVNPSQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGG 120
           IYVNPSQFAPSEDLSTYPSDF+GDIRKLMAVPGG+  VFHP+NLYDYGV++A    GNG 
Sbjct: 61  IYVNPSQFAPSEDLSTYPSDFDGDIRKLMAVPGGVDAVFHPYNLYDYGVESARC--GNGV 120

Query: 121 GSDGRAAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAV 180
            S+G A VS LE SGSGHETWVRVERLEKGMCG  RPVFFRGVATIVAKLFNIVEPDVAV
Sbjct: 121 VSNGTAVVSCLEESGSGHETWVRVERLEKGMCGVRRPVFFRGVATIVAKLFNIVEPDVAV 180

Query: 181 FGKKDYQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRS 240
           FGKKDYQQWRIIRRMVRDLDFSI +VGSEI+RD DGLA+SSRNV LS EERQKALSINRS
Sbjct: 181 FGKKDYQQWRIIRRMVRDLDFSINIVGSEIMRDVDGLAMSSRNVGLSLEERQKALSINRS 240

Query: 241 LSKAKSAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAA 300
           LSKAKSAAERGE NCK L++++VDEI EAGG LDY EIVEQESLEVVE+IKSPVV LIAA
Sbjct: 241 LSKAKSAAERGELNCKSLKSLVVDEIREAGGELDYVEIVEQESLEVVEQIKSPVVILIAA 300

Query: 301 WFGKVRLIDNMEIKL 315
           WFGKVRLIDN+EI +
Sbjct: 301 WFGKVRLIDNIEISI 313

BLAST of MC06g1753 vs. TAIR 10
Match: AT5G48840.1 (homolog of bacterial PANC )

HSP 1 Score: 411.0 bits (1055), Expect = 8.7e-115
Identity = 203/310 (65.48%), Postives = 251/310 (80.97%), Query Frame = 0

Query: 6   KPKIIIDKNQMRTWCRAMRSQGKTIALVPTMGYLHDGHLSLIEEAHKHTHLVVVSIYVNP 65
           +P++I DK+ MR W RAMRSQGKTI LVPTMGYLH+GHLSL+ ++   T + VVSIYVNP
Sbjct: 2   EPEVIRDKDSMRKWSRAMRSQGKTIGLVPTMGYLHEGHLSLVRQSLALTDVTVVSIYVNP 61

Query: 66  SQFAPSEDLSTYPSDFEGDIRKLMAVPGGIHVVFHPHNLYDYGVDAAVEFGGNGGGSDGR 125
            QF+P+EDLSTYPSDF GD+ KL A+ GG  VVF+P NLYDYG +       N GG +G 
Sbjct: 62  GQFSPTEDLSTYPSDFSGDLTKLAALSGGKVVVFNPKNLYDYGGETKKI---NDGGGNGG 121

Query: 126 AAVSSLEASGSGHETWVRVERLEKGMCGRSRPVFFRGVATIVAKLFNIVEPDVAVFGKKD 185
             VS +E  G GHETW+RVERLEKG CG+SRPVFFRGVATIV KLFNIVEPDVA+FGKKD
Sbjct: 122 RVVSCVEEGGLGHETWIRVERLEKGFCGKSRPVFFRGVATIVTKLFNIVEPDVALFGKKD 181

Query: 186 YQQWRIIRRMVRDLDFSIKVVGSEIVRDADGLALSSRNVHLSPEERQKALSINRSLSKAK 245
           YQQWRII+RMVRDL+F I++VGS+I R+ DGLA+SSRNV LS EERQ+ALSI+RSL+ AK
Sbjct: 182 YQQWRIIQRMVRDLNFGIEIVGSDIAREKDGLAMSSRNVRLSDEERQRALSISRSLAMAK 241

Query: 246 SAAERGERNCKKLQNMMVDEILEAGGRLDYAEIVEQESLEVVEEIKSPVVFLIAAWFGKV 305
           ++   G+ NC +L++M++ +++ + GR+DY EIV+QE+LE VEEIKS VV  +AAWFG V
Sbjct: 242 ASVAEGKTNCAELKDMIIQQVVGSAGRVDYVEIVDQETLEGVEEIKSGVVICVAAWFGTV 301

Query: 306 RLIDNMEIKL 316
           RLIDN+EI +
Sbjct: 302 RLIDNIEINV 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O240357.6e-12469.97Pantoate--beta-alanine ligase OS=Lotus japonicus OX=34305 GN=PANC PE=1 SV=3[more]
Q9FKB31.2e-11365.48Pantoate--beta-alanine ligase OS=Arabidopsis thaliana OX=3702 GN=At5g48840 PE=1 ... [more]
O242101.0e-10764.04Pantoate--beta-alanine ligase OS=Oryza sativa subsp. japonica OX=39947 GN=PANC P... [more]
Q2IFU31.5e-6646.77Pantothenate synthetase OS=Anaeromyxobacter dehalogenans (strain 2CP-C) OX=29039... [more]
B1L8434.7e-6545.72Pantothenate synthetase OS=Thermotoga sp. (strain RQ2) OX=126740 GN=panC PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022156035.11.66e-223100.00pantoate--beta-alanine ligase [Momordica charantia][more]
XP_022931128.13.52e-19386.35pantoate--beta-alanine ligase-like [Cucurbita moschata][more]
KAG6606322.11.01e-19286.03Pantoate--beta-alanine ligase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023533404.15.57e-19085.08pantoate--beta-alanine ligase-like [Cucurbita pepo subsp. pepo][more]
XP_038886857.14.10e-18986.71pantoate--beta-alanine ligase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DP648.03e-224100.00Pantoate--beta-alanine ligase OS=Momordica charantia OX=3673 GN=LOC111023004 PE=... [more]
A0A6J1EXI41.71e-19386.35Pantoate--beta-alanine ligase OS=Cucurbita moschata OX=3662 GN=LOC111437399 PE=3... [more]
A0A6J1K6E06.34e-18984.13Pantoate--beta-alanine ligase OS=Cucurbita maxima OX=3661 GN=LOC111491569 PE=3 S... [more]
A0A6J1HU159.31e-18685.71Pantoate--beta-alanine ligase OS=Cucurbita maxima OX=3661 GN=LOC111466858 PE=3 S... [more]
A0A6J1HLJ51.54e-18485.40Pantoate--beta-alanine ligase OS=Cucurbita moschata OX=3662 GN=LOC111464073 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G48840.18.7e-11565.48homolog of bacterial PANC [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 241..261
NoneNo IPR availablePANTHERPTHR21299CYTIDYLATE KINASE/PANTOATE-BETA-ALANINE LIGASEcoord: 6..313
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 7..313
IPR042176Pantoate-beta-alanine ligase, C-terminal domainGENE3D3.30.1300.10coord: 213..315
e-value: 5.4E-32
score: 112.5
IPR003721Pantoate-beta-alanine ligasePFAMPF02569Pantoate_ligasecoord: 9..311
e-value: 2.1E-95
score: 319.0
IPR003721Pantoate-beta-alanine ligaseTIGRFAMTIGR00018TIGR00018coord: 9..313
e-value: 1.7E-89
score: 297.5
IPR003721Pantoate-beta-alanine ligasePANTHERPTHR21299:SF1PANTOATE--BETA-ALANINE LIGASEcoord: 6..313
IPR003721Pantoate-beta-alanine ligaseHAMAPMF_00158PanCcoord: 8..313
score: 35.237091
IPR003721Pantoate-beta-alanine ligaseCDDcd00560PanCcoord: 7..311
e-value: 3.82613E-143
score: 402.683
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 5..212
e-value: 8.1E-73
score: 245.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1753.1MC06g1753.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015940 pantothenate biosynthetic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0004592 pantoate-beta-alanine ligase activity