MC06g1716 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1716
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAmino acid transporter, putative
LocationMC06: 24561892 .. 24563352 (-)
RNA-Seq ExpressionMC06g1716
SyntenyMC06g1716
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGTGGTTTTTGCAGAAATTTAGAACTTCCCCAGCCAGACAAGCGATGGGAGAGCTCAATCGTGTAGAGTATGTGTCGGTTGGCGAAACGCCAACTCCTTCAGCTCATAATGTAAAGAAACTTTCCGTACTGCCACTTGTGTTTCTCATTTTCTATGAGGTTTCTGGCGGTCCATTTGGAGTTGAGGACAGTGTTGGGGCAGCTGGCCCTCTGTTGGCTCTTCTTGGGTTCCTGGTCTTTCCATTTATATGGAGTATTCCTGAAGCATTGATTACTGCTGAGATGGGGACTATGTTCCCTGAAAATGGTGGTTATGTTGTTTGGGTTTCTTCTGCCTTGGGTCCGTTTTGGGGGTTTCAGCAGGGATGGATGAAATGGCTTAGTGGAGTTATTGATAATGCTTTATATCCTGTGTTGTTTCTTGATTATTTGGAGTCAGAGATCCCAGCTCTTGGCGGTGGTTTTCCAAGAGTAGCTGCTGTCTTGGCTTTGACGGTGATCCTCACTTACATGAATTATAGAGGATTAACAATTGTTGGTTGGGTTGCGGTAGTCCTAGGTGTTTTCTCAATCCTCCCTTTTGCGGTTATGGGACTCGTGTCTATTCCCAATCTCCGGCCAGATGGATGGCTCGTGGTGAACCTGCGAGATGTTGACTGGAATCTGTATTTGAACACTCTTTTCTGGAATTTGAATTATTGGGATTCGATTAGTACTTTGGCTGGAGAAGTGGATAACCCAAACAAAACACTTCCTAAAGGACTGTTTTATGCTTTGATTTTGGTTGTTCTTGGGTACTTTTTGCCTCTTTTAAGTGGGACAGGAGCTATTCCGCTTAATCGTGACTCATGGACTGACGGCTACTTTTCTGATGTTGCCAAAATGATTGGAGGGGCTTGGTTGAGTTGGTGGGTCCAAGGGGCTGCTGCCATGTCGAATATGGGAATGTTTGTGGCTGAGATGAGCAGTGATTCTTTCCAACTTCTTGGAATGGCAGAACGCGGTATGCTGCCCGAGTTCTTCAGCAAGCGATCTCGTCATGGGACACCATTGATTGGGATACTGTTCTCAGCCTCAGGTGTTGTCTTGCTTTCATGGTTGAGCTTCCAAGAAATCGTAGCAGCAGAGAACTTCTTGTACTGTTTTGGGATGATTTTGGAATTCATAGCGTTCGTAAAGCTAAGGATTAAACATCCAGCTGCATCACGACCTTACAAGATTCCAGTGGGAACCGCTGGATCAATCCTGATGTGCGTCCCTCCAACAATATTAATATGCATAGTGCTGGCCCTTTCAACAGCCAAGGTGATGATTGTGAGTCTAGTTGCTGTGGCAATTGGCGTAATGTTGCACCCTGGTCTCAAGTATGTTGAAAAGAAGAGATGGCTCAAATTCTCAGTCAGTTCTGACCTCCCTGATCTTCATTTCGCCAACCGGGACCGCACAGACACCTTAGTATAT

mRNA sequence

CTGTGGTTTTTGCAGAAATTTAGAACTTCCCCAGCCAGACAAGCGATGGGAGAGCTCAATCGTGTAGAGTATGTGTCGGTTGGCGAAACGCCAACTCCTTCAGCTCATAATGTAAAGAAACTTTCCGTACTGCCACTTGTGTTTCTCATTTTCTATGAGGTTTCTGGCGGTCCATTTGGAGTTGAGGACAGTGTTGGGGCAGCTGGCCCTCTGTTGGCTCTTCTTGGGTTCCTGGTCTTTCCATTTATATGGAGTATTCCTGAAGCATTGATTACTGCTGAGATGGGGACTATGTTCCCTGAAAATGGTGGTTATGTTGTTTGGGTTTCTTCTGCCTTGGGTCCGTTTTGGGGGTTTCAGCAGGGATGGATGAAATGGCTTAGTGGAGTTATTGATAATGCTTTATATCCTGTGTTGTTTCTTGATTATTTGGAGTCAGAGATCCCAGCTCTTGGCGGTGGTTTTCCAAGAGTAGCTGCTGTCTTGGCTTTGACGGTGATCCTCACTTACATGAATTATAGAGGATTAACAATTGTTGGTTGGGTTGCGGTAGTCCTAGGTGTTTTCTCAATCCTCCCTTTTGCGGTTATGGGACTCGTGTCTATTCCCAATCTCCGGCCAGATGGATGGCTCGTGGTGAACCTGCGAGATGTTGACTGGAATCTGTATTTGAACACTCTTTTCTGGAATTTGAATTATTGGGATTCGATTAGTACTTTGGCTGGAGAAGTGGATAACCCAAACAAAACACTTCCTAAAGGACTGTTTTATGCTTTGATTTTGGTTGTTCTTGGGTACTTTTTGCCTCTTTTAAGTGGGACAGGAGCTATTCCGCTTAATCGTGACTCATGGACTGACGGCTACTTTTCTGATGTTGCCAAAATGATTGGAGGGGCTTGGTTGAGTTGGTGGGTCCAAGGGGCTGCTGCCATGTCGAATATGGGAATGTTTGTGGCTGAGATGAGCAGTGATTCTTTCCAACTTCTTGGAATGGCAGAACGCGGTATGCTGCCCGAGTTCTTCAGCAAGCGATCTCGTCATGGGACACCATTGATTGGGATACTGTTCTCAGCCTCAGGTGTTGTCTTGCTTTCATGGTTGAGCTTCCAAGAAATCGTAGCAGCAGAGAACTTCTTGTACTGTTTTGGGATGATTTTGGAATTCATAGCGTTCGTAAAGCTAAGGATTAAACATCCAGCTGCATCACGACCTTACAAGATTCCAGTGGGAACCGCTGGATCAATCCTGATGTGCGTCCCTCCAACAATATTAATATGCATAGTGCTGGCCCTTTCAACAGCCAAGGTGATGATTGTGAGTCTAGTTGCTGTGGCAATTGGCGTAATGTTGCACCCTGGTCTCAAGTATGTTGAAAAGAAGAGATGGCTCAAATTCTCAGTCAGTTCTGACCTCCCTGATCTTCATTTCGCCAACCGGGACCGCACAGACACCTTAGTATAT

Coding sequence (CDS)

CTGTGGTTTTTGCAGAAATTTAGAACTTCCCCAGCCAGACAAGCGATGGGAGAGCTCAATCGTGTAGAGTATGTGTCGGTTGGCGAAACGCCAACTCCTTCAGCTCATAATGTAAAGAAACTTTCCGTACTGCCACTTGTGTTTCTCATTTTCTATGAGGTTTCTGGCGGTCCATTTGGAGTTGAGGACAGTGTTGGGGCAGCTGGCCCTCTGTTGGCTCTTCTTGGGTTCCTGGTCTTTCCATTTATATGGAGTATTCCTGAAGCATTGATTACTGCTGAGATGGGGACTATGTTCCCTGAAAATGGTGGTTATGTTGTTTGGGTTTCTTCTGCCTTGGGTCCGTTTTGGGGGTTTCAGCAGGGATGGATGAAATGGCTTAGTGGAGTTATTGATAATGCTTTATATCCTGTGTTGTTTCTTGATTATTTGGAGTCAGAGATCCCAGCTCTTGGCGGTGGTTTTCCAAGAGTAGCTGCTGTCTTGGCTTTGACGGTGATCCTCACTTACATGAATTATAGAGGATTAACAATTGTTGGTTGGGTTGCGGTAGTCCTAGGTGTTTTCTCAATCCTCCCTTTTGCGGTTATGGGACTCGTGTCTATTCCCAATCTCCGGCCAGATGGATGGCTCGTGGTGAACCTGCGAGATGTTGACTGGAATCTGTATTTGAACACTCTTTTCTGGAATTTGAATTATTGGGATTCGATTAGTACTTTGGCTGGAGAAGTGGATAACCCAAACAAAACACTTCCTAAAGGACTGTTTTATGCTTTGATTTTGGTTGTTCTTGGGTACTTTTTGCCTCTTTTAAGTGGGACAGGAGCTATTCCGCTTAATCGTGACTCATGGACTGACGGCTACTTTTCTGATGTTGCCAAAATGATTGGAGGGGCTTGGTTGAGTTGGTGGGTCCAAGGGGCTGCTGCCATGTCGAATATGGGAATGTTTGTGGCTGAGATGAGCAGTGATTCTTTCCAACTTCTTGGAATGGCAGAACGCGGTATGCTGCCCGAGTTCTTCAGCAAGCGATCTCGTCATGGGACACCATTGATTGGGATACTGTTCTCAGCCTCAGGTGTTGTCTTGCTTTCATGGTTGAGCTTCCAAGAAATCGTAGCAGCAGAGAACTTCTTGTACTGTTTTGGGATGATTTTGGAATTCATAGCGTTCGTAAAGCTAAGGATTAAACATCCAGCTGCATCACGACCTTACAAGATTCCAGTGGGAACCGCTGGATCAATCCTGATGTGCGTCCCTCCAACAATATTAATATGCATAGTGCTGGCCCTTTCAACAGCCAAGGTGATGATTGTGAGTCTAGTTGCTGTGGCAATTGGCGTAATGTTGCACCCTGGTCTCAAGTATGTTGAAAAGAAGAGATGGCTCAAATTCTCAGTCAGTTCTGACCTCCCTGATCTTCATTTCGCCAACCGGGACCGCACAGACACCTTAGTATAT

Protein sequence

LWFLQKFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTLVY
Homology
BLAST of MC06g1716 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 5.3e-217
Identity = 365/474 (77.00%), Postives = 416/474 (87.76%), Query Frame = 0

Query: 18  ELNRVEYVSVGETPTPSA-----HNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLL 77
           E N V Y SVG    PS+       ++K+S+LPLVFLIFYEVSGGPFGVEDSV AAGPLL
Sbjct: 21  ENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLL 80

Query: 78  ALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 137
           ALLGF++FPFIWSIPEALITAEMGTM+PENGGYVVWVSSALGPFWGFQQGWMKWLSGVID
Sbjct: 81  ALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 140

Query: 138 NALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSIL 197
           NALYPVLFLDYL+S +PALG G PRVA++L LT++LTY+NYRGLTIVGWVAV++GVFSIL
Sbjct: 141 NALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSIL 200

Query: 198 PFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLP 257
           PFAVMGL+SIP L P  WLV++L +V+WNLYLNTLFWNLNYWDSISTLAGEV+NPN TLP
Sbjct: 201 PFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLP 260

Query: 258 KGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMS 317
           K LFY +ILV   Y  PLL+G GAIPL R+ WTDGYFSDVAK +GGAWL WWVQ AAA S
Sbjct: 261 KALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATS 320

Query: 318 NMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEI 377
           NMGMF+AEMSSDSFQLLGMAERGMLPEFF+KRSR+GTPL+GILFSASGVVLLSWLSFQEI
Sbjct: 321 NMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEI 380

Query: 378 VAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALS 437
           VAAEN LYC GMILEFIAFV++R+KHPAASRPYKIP+GT GSILMC+PPTILIC V+ALS
Sbjct: 381 VAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALS 440

Query: 438 TAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTLV 487
           + KV  VS+V + IG ++HP L ++++KRW+KFS+SSDLPDL    R+  +TL+
Sbjct: 441 SLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQQQTREYEETLI 494

BLAST of MC06g1716 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 9.1e-201
Identity = 330/447 (73.83%), Postives = 393/447 (87.92%), Query Frame = 0

Query: 28  GETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPFIWSIP 87
           G+   P   + + +S++PL+FLIFYEVSGGPFG+EDSVGAAGPLLA++GFLV P IWSIP
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIWSIP 129

Query: 88  EALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLESE 147
           EALITAE+G MFPENGGYVVWV+SALGP+WGFQQGWMKWLSGVIDNALYPVLFLDYL+S 
Sbjct: 130 EALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSG 189

Query: 148 IPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPFAVMGLVSIPNLRP 207
           +PALGGG PR  AV+ LT +LT +NYRGLT+VGWVA+ LGVFS+LPF VMGL+++P LRP
Sbjct: 190 VPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRP 249

Query: 208 DGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKGLFYALILVVLGYF 267
             WLV++L +VDWNLYLNTLFWNLNYWDSISTLAGEV NP KTLPK LFYA+I VV+ Y 
Sbjct: 250 ARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYL 309

Query: 268 LPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMSNMGMFVAEMSSDSFQ 327
            PLL+GTGA+PL+R  WTDGYF+D+AK++GGAWL WWVQ AAA+SNMGMFVAEMSSDS+Q
Sbjct: 310 YPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQ 369

Query: 328 LLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIVAAENFLYCFGMILE 387
           LLGMAERGMLP FF+ RSR+GTPL GILFSASGV+LLS +SFQEIVAAENFLYCFGM+LE
Sbjct: 370 LLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLE 429

Query: 388 FIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALSTAKVMIVSLVAVAIG 447
           F+AF+  R++ P A+RPY++P+GTAG + M VPPT LI +VLALST KV +VSL AVA+G
Sbjct: 430 FVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMG 489

Query: 448 VMLHPGLKYVEKKRWLKFSVSSDLPDL 475
           ++L P L++VEKKRWL+FSV+ DLP++
Sbjct: 490 LVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of MC06g1716 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 9.1e-201
Identity = 330/447 (73.83%), Postives = 393/447 (87.92%), Query Frame = 0

Query: 28  GETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLGFLVFPFIWSIP 87
           G+   P   + + +S++PL+FLIFYEVSGGPFG+EDSVGAAGPLLA++GFLV P IWSIP
Sbjct: 70  GDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIWSIP 129

Query: 88  EALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLESE 147
           EALITAE+G MFPENGGYVVWV+SALGP+WGFQQGWMKWLSGVIDNALYPVLFLDYL+S 
Sbjct: 130 EALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSG 189

Query: 148 IPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPFAVMGLVSIPNLRP 207
           +PALGGG PR  AV+ LT +LT +NYRGLT+VGWVA+ LGVFS+LPF VMGL+++P LRP
Sbjct: 190 VPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRP 249

Query: 208 DGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKGLFYALILVVLGYF 267
             WLV++L +VDWNLYLNTLFWNLNYWDSISTLAGEV NP KTLPK LFYA+I VV+ Y 
Sbjct: 250 ARWLVIDLHNVDWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYL 309

Query: 268 LPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMSNMGMFVAEMSSDSFQ 327
            PLL+GTGA+PL+R  WTDGYF+D+AK++GGAWL WWVQ AAA+SNMGMFVAEMSSDS+Q
Sbjct: 310 YPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQ 369

Query: 328 LLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIVAAENFLYCFGMILE 387
           LLGMAERGMLP FF+ RSR+GTPL GILFSASGV+LLS +SFQEIVAAENFLYCFGM+LE
Sbjct: 370 LLGMAERGMLPSFFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLE 429

Query: 388 FIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALSTAKVMIVSLVAVAIG 447
           F+AF+  R++ P A+RPY++P+GTAG + M VPPT LI +VLALST KV +VSL AVA+G
Sbjct: 430 FVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMG 489

Query: 448 VMLHPGLKYVEKKRWLKFSVSSDLPDL 475
           ++L P L++VEKKRWL+FSV+ DLP++
Sbjct: 490 LVLQPALRFVEKKRWLRFSVNPDLPEI 516

BLAST of MC06g1716 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 6.1e-197
Identity = 330/460 (71.74%), Postives = 395/460 (85.87%), Query Frame = 0

Query: 24  YVSVGET-------PTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLG 83
           ++S+G T       P  + + +KK++VLPLVFLIFYEVSGGPFG+EDSV AAGPLLA++G
Sbjct: 29  HISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVG 88

Query: 84  FLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALY 143
           F+VFPFIWSIPEALITAEMGTMFPENGGYVVWV+ A+GP+WGFQQGW+KWLSGVIDNALY
Sbjct: 89  FIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALY 148

Query: 144 PVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPFAV 203
           P+LFLDYL+S IP LG G PRVAA+L LTV LTY+NYRGL+IVG  AV+LGVFSILPF V
Sbjct: 149 PILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVV 208

Query: 204 MGLVSIPNLRPDGWLVVN--LRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKG 263
           M  +SIP L+P  WLVV+  ++ V+W+LYLNTLFWNLNYWDS+STL GEV+NP+KTLP+ 
Sbjct: 209 MSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRA 268

Query: 264 LFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMSNM 323
           LFYAL+LVV  Y  P+L+GTGAI L++  WTDGYF+D+ K+IGG WL WW+Q AAA SNM
Sbjct: 269 LFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNM 328

Query: 324 GMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIVA 383
           GMF+AEMSSDSFQLLGMAERGMLPE F+KRSR+ TP +GILFSASGV++LSWLSFQEIVA
Sbjct: 329 GMFLAEMSSDSFQLLGMAERGMLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVA 388

Query: 384 AENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALSTA 443
           AEN LYCFGM+LEFI FV+LR+K+PAASRP+KIPVG  GS+LMC+PPT+LI +++A +  
Sbjct: 389 AENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNL 448

Query: 444 KVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDL 475
           KV +VSL A+ IG++L P LK VEKK WLKFS SS LP+L
Sbjct: 449 KVALVSLAAIVIGLVLQPCLKQVEKKGWLKFSTSSHLPNL 488

BLAST of MC06g1716 vs. ExPASy Swiss-Prot
Match: Q9C6S4 (Probable polyamine transporter At1g31820 OS=Arabidopsis thaliana OX=3702 GN=At1g31820 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 9.1e-193
Identity = 335/472 (70.97%), Postives = 390/472 (82.63%), Query Frame = 0

Query: 15  AMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLAL 74
           A G L   +   VG +     ++++K+S+LPLVFLIFYEVSGGPFG E SV AAGPLLAL
Sbjct: 10  AYGNLYDDDDGDVGGSSKEGNNSIQKVSMLPLVFLIFYEVSGGPFGAEGSVNAAGPLLAL 69

Query: 75  LGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 134
           LGF++FPFIW IPEALITAEM TMFP NGG+VVWVSSALG FWGFQ GWMKWL GVIDNA
Sbjct: 70  LGFVIFPFIWCIPEALITAEMSTMFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNA 129

Query: 135 LYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPF 194
           LYPVLFLDYL+S +PAL  G PRVA++L LT++LTY+NYRGLTIVGW AV +GVFS+LPF
Sbjct: 130 LYPVLFLDYLKSAVPALATGLPRVASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPF 189

Query: 195 AVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKG 254
           AVM LVSIP L P  WLV++L +V+WNLYLNTL WNLNYWDS+STLAGEV NP KTLPK 
Sbjct: 190 AVMSLVSIPQLEPSRWLVMDLGNVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKA 249

Query: 255 LFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMSNM 314
           L Y +I V L  FLPLLSGTGAIPL+R+ WTDGY ++VAK IGG WL  WVQ AAA SNM
Sbjct: 250 LCYGVIFVALSNFLPLLSGTGAIPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNM 309

Query: 315 GMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIVA 374
           GMF+AEMSSDSFQLLGMAE G+LPE F++RSR+GTPL+GILFSASGV+LLS LSFQEI+A
Sbjct: 310 GMFLAEMSSDSFQLLGMAELGILPEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIA 369

Query: 375 AENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALSTA 434
           AEN LYC GMILEFIAFV+LR KHPAASRPYKIPVGT GSIL+CVPP +LIC+V+ LST 
Sbjct: 370 AENLLYCGGMILEFIAFVRLRKKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTI 429

Query: 435 KVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTLV 487
           KV +VS V V IG ++ P L +++ K+W+KFSV SDL +    N D  ++L+
Sbjct: 430 KVALVSFVMVVIGFLMKPCLNHMDGKKWVKFSVCSDLAEFQKENLDCEESLL 481

BLAST of MC06g1716 vs. NCBI nr
Match: XP_022159490.1 (probable polyamine transporter At1g31830 isoform X1 [Momordica charantia])

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 5   QKFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDS 64
           QKFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDS
Sbjct: 65  QKFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDS 124

Query: 65  VGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWM 124
           VGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWM
Sbjct: 125 VGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWM 184

Query: 125 KWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAV 184
           KWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAV
Sbjct: 185 KWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAV 244

Query: 185 VLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEV 244
           VLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEV
Sbjct: 245 VLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEV 304

Query: 245 DNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWW 304
           DNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWW
Sbjct: 305 DNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWW 364

Query: 305 VQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLL 364
           VQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLL
Sbjct: 365 VQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLL 424

Query: 365 SWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTIL 424
           SWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTIL
Sbjct: 425 SWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTIL 484

Query: 425 ICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDT 484
           ICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDT
Sbjct: 485 ICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDT 544

Query: 485 LVY 487
           LVY
Sbjct: 545 LVY 547

BLAST of MC06g1716 vs. NCBI nr
Match: XP_022159491.1 (probable polyamine transporter At1g31830 isoform X2 [Momordica charantia])

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 482/482 (100.00%), Postives = 482/482 (100.00%), Query Frame = 0

Query: 6   KFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSV 65
           KFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSV
Sbjct: 2   KFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSV 61

Query: 66  GAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMK 125
           GAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMK
Sbjct: 62  GAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMK 121

Query: 126 WLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVV 185
           WLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVV
Sbjct: 122 WLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVV 181

Query: 186 LGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVD 245
           LGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVD
Sbjct: 182 LGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVD 241

Query: 246 NPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWV 305
           NPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWV
Sbjct: 242 NPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWV 301

Query: 306 QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLS 365
           QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLS
Sbjct: 302 QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLS 361

Query: 366 WLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILI 425
           WLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILI
Sbjct: 362 WLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILI 421

Query: 426 CIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTL 485
           CIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTL
Sbjct: 422 CIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTL 481

Query: 486 VY 487
           VY
Sbjct: 482 VY 483

BLAST of MC06g1716 vs. NCBI nr
Match: XP_038887706.1 (probable polyamine transporter At1g31830 isoform X1 [Benincasa hispida])

HSP 1 Score: 900 bits (2325), Expect = 0.0
Identity = 446/487 (91.58%), Postives = 466/487 (95.69%), Query Frame = 0

Query: 4   LQKFRTSPARQA---MGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFG 63
           LQK RTSPARQA   MGE+NR EYVSVGE+P+PS  N KK+SVLPLVFLIFYEVSGGPFG
Sbjct: 61  LQKLRTSPARQASVAMGEINRAEYVSVGESPSPSISNAKKVSVLPLVFLIFYEVSGGPFG 120

Query: 64  VEDSVGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ 123
           VEDSVGAAGPLLALLGFLVFP IWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ
Sbjct: 121 VEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ 180

Query: 124 QGWMKWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVG 183
           QGWMKWLSGVIDNALYPVLFLDYL+SEIPALGGG PRVAAVLALTVILTY+NYRGLTIVG
Sbjct: 181 QGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVILTYLNYRGLTIVG 240

Query: 184 WVAVVLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTL 243
           WVAV+LGVFSILPFAVMGLVSIP LRP  WLVVNL+DVDWNLYLNTLFWNLNYWDSISTL
Sbjct: 241 WVAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTL 300

Query: 244 AGEVDNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAW 303
           AGEV+NPNKTLPK LFYALILVVL YFLPLLSGTGAI LNR+ WTDGYFSDVAK+IGGAW
Sbjct: 301 AGEVENPNKTLPKALFYALILVVLSYFLPLLSGTGAIVLNRELWTDGYFSDVAKIIGGAW 360

Query: 304 LSWWVQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG 363
           L WW+QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG
Sbjct: 361 LGWWIQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG 420

Query: 364 VVLLSWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVP 423
           VVLLSWLSFQEIVAAENFLYCFGMILEF+AF+KLRIKHPAASRPYKIPVGTAGSILMC+P
Sbjct: 421 VVLLSWLSFQEIVAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTAGSILMCIP 480

Query: 424 PTILICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRD 483
           PTILICIVLALST KVMIVSL AVAIG++L PGLKYVEKKRWLKFSVS+DLPDLHFANR+
Sbjct: 481 PTILICIVLALSTVKVMIVSLAAVAIGLLLQPGLKYVEKKRWLKFSVSADLPDLHFANRN 540

Query: 484 RTDTLVY 487
           R+DTLVY
Sbjct: 541 RSDTLVY 547

BLAST of MC06g1716 vs. NCBI nr
Match: KAG6606291.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 442/486 (90.95%), Postives = 462/486 (95.06%), Query Frame = 0

Query: 5   QKFRTSPARQ---AMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGV 64
           QKF TSPARQ   AMGELNR EYVSVGE+P+   +N KK+SVLPLVFLIFYEVSGGPFGV
Sbjct: 76  QKFSTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGV 135

Query: 65  EDSVGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQ 124
           EDSVGAAGPLLALLGFLVFP IWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQ
Sbjct: 136 EDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQ 195

Query: 125 GWMKWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGW 184
           GWMKWLSGVIDNALYPVLFLDYL+SEIPALGGG PRVAAVLALTV+LTYMNYRGLTIVGW
Sbjct: 196 GWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGW 255

Query: 185 VAVVLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLA 244
           VAV+LGVFSILPFAVMGLVSIP LRP  WLVVNL+DVDWNLYLNTLFWNLNYWDSISTLA
Sbjct: 256 VAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLA 315

Query: 245 GEVDNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWL 304
           GEV+NPNKTLPK LFYALILVVLGYF PLLSGTGAIPLNRD WTDGYFSDVAK+IGGAWL
Sbjct: 316 GEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWL 375

Query: 305 SWWVQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGV 364
           SWW+ GAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFF KRSRHGTPLIGILFSASGV
Sbjct: 376 SWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGV 435

Query: 365 VLLSWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPP 424
           VLLSWLSFQEI+AAENFLYCFGMILEF+AF+KLRIKHPAASRPYKIPVGT GSILMC+PP
Sbjct: 436 VLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPP 495

Query: 425 TILICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDR 484
           TILIC+VLALST KVMIVSL AVA+G++LHPGL YVEKKRWLKFSVS+DLPDLHFANRDR
Sbjct: 496 TILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDR 555

Query: 485 TDTLVY 487
            DTLVY
Sbjct: 556 PDTLVY 561

BLAST of MC06g1716 vs. NCBI nr
Match: KAG7036233.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 898 bits (2320), Expect = 0.0
Identity = 442/486 (90.95%), Postives = 462/486 (95.06%), Query Frame = 0

Query: 5   QKFRTSPARQ---AMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGV 64
           QKF TSPARQ   AMGELNR EYVSVGE+P+   +N KK+SVLPLVFLIFYEVSGGPFGV
Sbjct: 76  QKFGTSPARQVSVAMGELNRAEYVSVGESPSSPVNNAKKVSVLPLVFLIFYEVSGGPFGV 135

Query: 65  EDSVGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQ 124
           EDSVGAAGPLLALLGFLVFP IWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQ
Sbjct: 136 EDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQ 195

Query: 125 GWMKWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGW 184
           GWMKWLSGVIDNALYPVLFLDYL+SEIPALGGG PRVAAVLALTV+LTYMNYRGLTIVGW
Sbjct: 196 GWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVVLTYMNYRGLTIVGW 255

Query: 185 VAVVLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLA 244
           VAV+LGVFSILPFAVMGLVSIP LRP  WLVVNL+DVDWNLYLNTLFWNLNYWDSISTLA
Sbjct: 256 VAVILGVFSILPFAVMGLVSIPKLRPSRWLVVNLKDVDWNLYLNTLFWNLNYWDSISTLA 315

Query: 245 GEVDNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWL 304
           GEV+NPNKTLPK LFYALILVVLGYF PLLSGTGAIPLNRD WTDGYFSDVAK+IGGAWL
Sbjct: 316 GEVENPNKTLPKALFYALILVVLGYFFPLLSGTGAIPLNRDLWTDGYFSDVAKIIGGAWL 375

Query: 305 SWWVQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGV 364
           SWW+ GAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFF KRSRHGTPLIGILFSASGV
Sbjct: 376 SWWITGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFGKRSRHGTPLIGILFSASGV 435

Query: 365 VLLSWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPP 424
           VLLSWLSFQEI+AAENFLYCFGMILEF+AF+KLRIKHPAASRPYKIPVGT GSILMC+PP
Sbjct: 436 VLLSWLSFQEIIAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTVGSILMCIPP 495

Query: 425 TILICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDR 484
           TILIC+VLALST KVMIVSL AVA+G++LHPGL YVEKKRWLKFSVS+DLPDLHFANRDR
Sbjct: 496 TILICVVLALSTVKVMIVSLAAVAVGLLLHPGLGYVEKKRWLKFSVSADLPDLHFANRDR 555

Query: 485 TDTLVY 487
            DTLVY
Sbjct: 556 PDTLVY 561

BLAST of MC06g1716 vs. ExPASy TrEMBL
Match: A0A6J1DYW6 (probable polyamine transporter At1g31830 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025891 PE=3 SV=1)

HSP 1 Score: 967 bits (2501), Expect = 0.0
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 5   QKFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDS 64
           QKFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDS
Sbjct: 65  QKFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDS 124

Query: 65  VGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWM 124
           VGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWM
Sbjct: 125 VGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWM 184

Query: 125 KWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAV 184
           KWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAV
Sbjct: 185 KWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAV 244

Query: 185 VLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEV 244
           VLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEV
Sbjct: 245 VLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEV 304

Query: 245 DNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWW 304
           DNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWW
Sbjct: 305 DNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWW 364

Query: 305 VQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLL 364
           VQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLL
Sbjct: 365 VQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLL 424

Query: 365 SWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTIL 424
           SWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTIL
Sbjct: 425 SWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTIL 484

Query: 425 ICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDT 484
           ICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDT
Sbjct: 485 ICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDT 544

Query: 485 LVY 487
           LVY
Sbjct: 545 LVY 547

BLAST of MC06g1716 vs. ExPASy TrEMBL
Match: A0A6J1E433 (probable polyamine transporter At1g31830 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025891 PE=3 SV=1)

HSP 1 Score: 966 bits (2496), Expect = 0.0
Identity = 482/482 (100.00%), Postives = 482/482 (100.00%), Query Frame = 0

Query: 6   KFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSV 65
           KFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSV
Sbjct: 2   KFRTSPARQAMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSV 61

Query: 66  GAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMK 125
           GAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMK
Sbjct: 62  GAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMK 121

Query: 126 WLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVV 185
           WLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVV
Sbjct: 122 WLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVV 181

Query: 186 LGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVD 245
           LGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVD
Sbjct: 182 LGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVD 241

Query: 246 NPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWV 305
           NPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWV
Sbjct: 242 NPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWV 301

Query: 306 QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLS 365
           QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLS
Sbjct: 302 QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLS 361

Query: 366 WLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILI 425
           WLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILI
Sbjct: 362 WLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILI 421

Query: 426 CIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTL 485
           CIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTL
Sbjct: 422 CIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTL 481

Query: 486 VY 487
           VY
Sbjct: 482 VY 483

BLAST of MC06g1716 vs. ExPASy TrEMBL
Match: A0A0A0LBS3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G636430 PE=3 SV=1)

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 447/491 (91.04%), Postives = 465/491 (94.70%), Query Frame = 0

Query: 1   LWFLQKFRTSPARQA---MGELNRVEYVSVGETPTPSA-HNVKKLSVLPLVFLIFYEVSG 60
           LW  QK RTSPARQA   MGE+NR EYVSVGE+P+P    N KK+SVLPLVFLIFYEVSG
Sbjct: 16  LWIEQKLRTSPARQASVSMGEINRAEYVSVGESPSPPVVSNAKKVSVLPLVFLIFYEVSG 75

Query: 61  GPFGVEDSVGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPF 120
           GPFGVEDSVGAAGPLLALLGFLVFP IWSIPEALITAEMGTMFPENGGYVVWVSSALGPF
Sbjct: 76  GPFGVEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPF 135

Query: 121 WGFQQGWMKWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGL 180
           WGFQQGWMKWLSGVIDNALYPVLFLDYL+SEIPALGGG PRVAAVLALTVILTYMNYRGL
Sbjct: 136 WGFQQGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVILTYMNYRGL 195

Query: 181 TIVGWVAVVLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDS 240
           TIVGWVAV+LGVFSILPFAVMGLVSIP LRP  W+VVNL+DVDWNLYLNTLFWNLNYWDS
Sbjct: 196 TIVGWVAVILGVFSILPFAVMGLVSIPKLRPARWVVVNLKDVDWNLYLNTLFWNLNYWDS 255

Query: 241 ISTLAGEVDNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMI 300
           ISTLAGEV+NPNKTLPK LFYALILVVL YFLPLLSGTGAI LNR+ WTDGYFSDVAK+I
Sbjct: 256 ISTLAGEVENPNKTLPKALFYALILVVLSYFLPLLSGTGAIALNRELWTDGYFSDVAKII 315

Query: 301 GGAWLSWWVQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILF 360
           GGAWL WW+QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILF
Sbjct: 316 GGAWLGWWIQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILF 375

Query: 361 SASGVVLLSWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSIL 420
           SASGVVLLSWLSFQEIVAAENFLYCFGMILEF+AF+KLRIKHPAASRPYKIPVGTAGSIL
Sbjct: 376 SASGVVLLSWLSFQEIVAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTAGSIL 435

Query: 421 MCVPPTILICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHF 480
           MC+PPTILICIVLALST KVMIVSL AVAIG++L PGLKYVEKKRWLKFSVS+DLPDLHF
Sbjct: 436 MCIPPTILICIVLALSTVKVMIVSLAAVAIGLLLQPGLKYVEKKRWLKFSVSADLPDLHF 495

Query: 481 ANRDRTDTLVY 487
           ANRDR DTLVY
Sbjct: 496 ANRDRPDTLVY 506

BLAST of MC06g1716 vs. ExPASy TrEMBL
Match: A0A1S4E2U0 (probable polyamine transporter At1g31830 isoform X5 OS=Cucumis melo OX=3656 GN=LOC103499237 PE=3 SV=1)

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 444/487 (91.17%), Postives = 464/487 (95.28%), Query Frame = 0

Query: 5   QKFRTSPARQ---AMGELNRVEYVSVGETPTPSA-HNVKKLSVLPLVFLIFYEVSGGPFG 64
           QK RTSPARQ   +MGE+NR EYVSVGE+P+P+   N KK+SVLPLVFLIFYEVSGGPFG
Sbjct: 46  QKLRTSPARQTSVSMGEINRAEYVSVGESPSPAVVSNAKKVSVLPLVFLIFYEVSGGPFG 105

Query: 65  VEDSVGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ 124
           VEDSVGAAGPLLALLGFLVFP IWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ
Sbjct: 106 VEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ 165

Query: 125 QGWMKWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVG 184
           QGWMKWLSGVIDNALYPVLFLDYL+SEIPALGGG PRVAAVLALTVILTYMNYRGLTIVG
Sbjct: 166 QGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVILTYMNYRGLTIVG 225

Query: 185 WVAVVLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTL 244
           WVAV+LGVFSILPFAVMGLVSIP LRP  W+VVNL+DVDWNLYLNTLFWNLNYWDSISTL
Sbjct: 226 WVAVILGVFSILPFAVMGLVSIPKLRPARWVVVNLKDVDWNLYLNTLFWNLNYWDSISTL 285

Query: 245 AGEVDNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAW 304
           AGEV+NPNKTLPK LFYALILVVL YFLPLLSGTGAI LNR+ WTDGYFSDVAK+IGGAW
Sbjct: 286 AGEVENPNKTLPKALFYALILVVLSYFLPLLSGTGAIALNRELWTDGYFSDVAKIIGGAW 345

Query: 305 LSWWVQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG 364
           L WW+QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG
Sbjct: 346 LGWWIQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG 405

Query: 365 VVLLSWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVP 424
           VVLLSWLSFQEIVAAENFLYCFGMILEF+AF+KLRIKHPAASRPYKIPVGTAGSILMC+P
Sbjct: 406 VVLLSWLSFQEIVAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTAGSILMCIP 465

Query: 425 PTILICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRD 484
           PTILICIVLALST KVMIVSL AVAIG++L PGLKYVEKKRWLKFSVS+DLPDLHFANRD
Sbjct: 466 PTILICIVLALSTVKVMIVSLAAVAIGLLLQPGLKYVEKKRWLKFSVSADLPDLHFANRD 525

Query: 485 RTDTLVY 487
           R DTLVY
Sbjct: 526 RPDTLVY 532

BLAST of MC06g1716 vs. ExPASy TrEMBL
Match: A0A1S3CCI9 (probable polyamine transporter At1g31830 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499237 PE=3 SV=1)

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 444/487 (91.17%), Postives = 464/487 (95.28%), Query Frame = 0

Query: 5   QKFRTSPARQ---AMGELNRVEYVSVGETPTPSA-HNVKKLSVLPLVFLIFYEVSGGPFG 64
           QK RTSPARQ   +MGE+NR EYVSVGE+P+P+   N KK+SVLPLVFLIFYEVSGGPFG
Sbjct: 114 QKLRTSPARQTSVSMGEINRAEYVSVGESPSPAVVSNAKKVSVLPLVFLIFYEVSGGPFG 173

Query: 65  VEDSVGAAGPLLALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ 124
           VEDSVGAAGPLLALLGFLVFP IWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ
Sbjct: 174 VEDSVGAAGPLLALLGFLVFPLIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQ 233

Query: 125 QGWMKWLSGVIDNALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVG 184
           QGWMKWLSGVIDNALYPVLFLDYL+SEIPALGGG PRVAAVLALTVILTYMNYRGLTIVG
Sbjct: 234 QGWMKWLSGVIDNALYPVLFLDYLKSEIPALGGGLPRVAAVLALTVILTYMNYRGLTIVG 293

Query: 185 WVAVVLGVFSILPFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTL 244
           WVAV+LGVFSILPFAVMGLVSIP LRP  W+VVNL+DVDWNLYLNTLFWNLNYWDSISTL
Sbjct: 294 WVAVILGVFSILPFAVMGLVSIPKLRPARWVVVNLKDVDWNLYLNTLFWNLNYWDSISTL 353

Query: 245 AGEVDNPNKTLPKGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAW 304
           AGEV+NPNKTLPK LFYALILVVL YFLPLLSGTGAI LNR+ WTDGYFSDVAK+IGGAW
Sbjct: 354 AGEVENPNKTLPKALFYALILVVLSYFLPLLSGTGAIALNRELWTDGYFSDVAKIIGGAW 413

Query: 305 LSWWVQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG 364
           L WW+QGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG
Sbjct: 414 LGWWIQGAAAMSNMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASG 473

Query: 365 VVLLSWLSFQEIVAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVP 424
           VVLLSWLSFQEIVAAENFLYCFGMILEF+AF+KLRIKHPAASRPYKIPVGTAGSILMC+P
Sbjct: 474 VVLLSWLSFQEIVAAENFLYCFGMILEFLAFIKLRIKHPAASRPYKIPVGTAGSILMCIP 533

Query: 425 PTILICIVLALSTAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRD 484
           PTILICIVLALST KVMIVSL AVAIG++L PGLKYVEKKRWLKFSVS+DLPDLHFANRD
Sbjct: 534 PTILICIVLALSTVKVMIVSLAAVAIGLLLQPGLKYVEKKRWLKFSVSADLPDLHFANRD 593

Query: 485 RTDTLVY 487
           R DTLVY
Sbjct: 594 RPDTLVY 600

BLAST of MC06g1716 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 755.0 bits (1948), Expect = 3.8e-218
Identity = 365/474 (77.00%), Postives = 416/474 (87.76%), Query Frame = 0

Query: 18  ELNRVEYVSVGETPTPSA-----HNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLL 77
           E N V Y SVG    PS+       ++K+S+LPLVFLIFYEVSGGPFGVEDSV AAGPLL
Sbjct: 21  ENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLL 80

Query: 78  ALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 137
           ALLGF++FPFIWSIPEALITAEMGTM+PENGGYVVWVSSALGPFWGFQQGWMKWLSGVID
Sbjct: 81  ALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 140

Query: 138 NALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSIL 197
           NALYPVLFLDYL+S +PALG G PRVA++L LT++LTY+NYRGLTIVGWVAV++GVFSIL
Sbjct: 141 NALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSIL 200

Query: 198 PFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLP 257
           PFAVMGL+SIP L P  WLV++L +V+WNLYLNTLFWNLNYWDSISTLAGEV+NPN TLP
Sbjct: 201 PFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLP 260

Query: 258 KGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMS 317
           K LFY +ILV   Y  PLL+G GAIPL R+ WTDGYFSDVAK +GGAWL WWVQ AAA S
Sbjct: 261 KALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATS 320

Query: 318 NMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEI 377
           NMGMF+AEMSSDSFQLLGMAERGMLPEFF+KRSR+GTPL+GILFSASGVVLLSWLSFQEI
Sbjct: 321 NMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEI 380

Query: 378 VAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALS 437
           VAAEN LYC GMILEFIAFV++R+KHPAASRPYKIP+GT GSILMC+PPTILIC V+ALS
Sbjct: 381 VAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALS 440

Query: 438 TAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTLV 487
           + KV  VS+V + IG ++HP L ++++KRW+KFS+SSDLPDL    R+  +TL+
Sbjct: 441 SLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQQQTREYEETLI 494

BLAST of MC06g1716 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 755.0 bits (1948), Expect = 3.8e-218
Identity = 365/474 (77.00%), Postives = 416/474 (87.76%), Query Frame = 0

Query: 18  ELNRVEYVSVGETPTPSA-----HNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLL 77
           E N V Y SVG    PS+       ++K+S+LPLVFLIFYEVSGGPFGVEDSV AAGPLL
Sbjct: 5   ENNEVPYSSVGADEVPSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLL 64

Query: 78  ALLGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 137
           ALLGF++FPFIWSIPEALITAEMGTM+PENGGYVVWVSSALGPFWGFQQGWMKWLSGVID
Sbjct: 65  ALLGFVIFPFIWSIPEALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVID 124

Query: 138 NALYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSIL 197
           NALYPVLFLDYL+S +PALG G PRVA++L LT++LTY+NYRGLTIVGWVAV++GVFSIL
Sbjct: 125 NALYPVLFLDYLKSGVPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSIL 184

Query: 198 PFAVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLP 257
           PFAVMGL+SIP L P  WLV++L +V+WNLYLNTLFWNLNYWDSISTLAGEV+NPN TLP
Sbjct: 185 PFAVMGLISIPQLEPSRWLVMDLGNVNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLP 244

Query: 258 KGLFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMS 317
           K LFY +ILV   Y  PLL+G GAIPL R+ WTDGYFSDVAK +GGAWL WWVQ AAA S
Sbjct: 245 KALFYGVILVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATS 304

Query: 318 NMGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEI 377
           NMGMF+AEMSSDSFQLLGMAERGMLPEFF+KRSR+GTPL+GILFSASGVVLLSWLSFQEI
Sbjct: 305 NMGMFIAEMSSDSFQLLGMAERGMLPEFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEI 364

Query: 378 VAAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALS 437
           VAAEN LYC GMILEFIAFV++R+KHPAASRPYKIP+GT GSILMC+PPTILIC V+ALS
Sbjct: 365 VAAENLLYCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALS 424

Query: 438 TAKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTLV 487
           + KV  VS+V + IG ++HP L ++++KRW+KFS+SSDLPDL    R+  +TL+
Sbjct: 425 SLKVAAVSIVMMIIGFLIHPLLNHMDRKRWVKFSISSDLPDLQQQTREYEETLI 478

BLAST of MC06g1716 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 688.3 bits (1775), Expect = 4.3e-198
Identity = 330/460 (71.74%), Postives = 395/460 (85.87%), Query Frame = 0

Query: 24  YVSVGET-------PTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLALLG 83
           ++S+G T       P  + + +KK++VLPLVFLIFYEVSGGPFG+EDSV AAGPLLA++G
Sbjct: 29  HISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPFGIEDSVKAAGPLLAIVG 88

Query: 84  FLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALY 143
           F+VFPFIWSIPEALITAEMGTMFPENGGYVVWV+ A+GP+WGFQQGW+KWLSGVIDNALY
Sbjct: 89  FIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALY 148

Query: 144 PVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPFAV 203
           P+LFLDYL+S IP LG G PRVAA+L LTV LTY+NYRGL+IVG  AV+LGVFSILPF V
Sbjct: 149 PILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVV 208

Query: 204 MGLVSIPNLRPDGWLVVN--LRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKG 263
           M  +SIP L+P  WLVV+  ++ V+W+LYLNTLFWNLNYWDS+STL GEV+NP+KTLP+ 
Sbjct: 209 MSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRA 268

Query: 264 LFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMSNM 323
           LFYAL+LVV  Y  P+L+GTGAI L++  WTDGYF+D+ K+IGG WL WW+Q AAA SNM
Sbjct: 269 LFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNM 328

Query: 324 GMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIVA 383
           GMF+AEMSSDSFQLLGMAERGMLPE F+KRSR+ TP +GILFSASGV++LSWLSFQEIVA
Sbjct: 329 GMFLAEMSSDSFQLLGMAERGMLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVA 388

Query: 384 AENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALSTA 443
           AEN LYCFGM+LEFI FV+LR+K+PAASRP+KIPVG  GS+LMC+PPT+LI +++A +  
Sbjct: 389 AENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNL 448

Query: 444 KVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDL 475
           KV +VSL A+ IG++L P LK VEKK WLKFS SS LP+L
Sbjct: 449 KVALVSLAAIVIGLVLQPCLKQVEKKGWLKFSTSSHLPNL 488

BLAST of MC06g1716 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 674.5 bits (1739), Expect = 6.5e-194
Identity = 335/472 (70.97%), Postives = 390/472 (82.63%), Query Frame = 0

Query: 15  AMGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSVGAAGPLLAL 74
           A G L   +   VG +     ++++K+S+LPLVFLIFYEVSGGPFG E SV AAGPLLAL
Sbjct: 10  AYGNLYDDDDGDVGGSSKEGNNSIQKVSMLPLVFLIFYEVSGGPFGAEGSVNAAGPLLAL 69

Query: 75  LGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 134
           LGF++FPFIW IPEALITAEM TMFP NGG+VVWVSSALG FWGFQ GWMKWL GVIDNA
Sbjct: 70  LGFVIFPFIWCIPEALITAEMSTMFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNA 129

Query: 135 LYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPF 194
           LYPVLFLDYL+S +PAL  G PRVA++L LT++LTY+NYRGLTIVGW AV +GVFS+LPF
Sbjct: 130 LYPVLFLDYLKSAVPALATGLPRVASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPF 189

Query: 195 AVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKG 254
           AVM LVSIP L P  WLV++L +V+WNLYLNTL WNLNYWDS+STLAGEV NP KTLPK 
Sbjct: 190 AVMSLVSIPQLEPSRWLVMDLGNVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKA 249

Query: 255 LFYALILVVLGYFLPLLSGTGAIPLNRDSWTDGYFSDVAKMIGGAWLSWWVQGAAAMSNM 314
           L Y +I V L  FLPLLSGTGAIPL+R+ WTDGY ++VAK IGG WL  WVQ AAA SNM
Sbjct: 250 LCYGVIFVALSNFLPLLSGTGAIPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNM 309

Query: 315 GMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIVA 374
           GMF+AEMSSDSFQLLGMAE G+LPE F++RSR+GTPL+GILFSASGV+LLS LSFQEI+A
Sbjct: 310 GMFLAEMSSDSFQLLGMAELGILPEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIA 369

Query: 375 AENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALSTA 434
           AEN LYC GMILEFIAFV+LR KHPAASRPYKIPVGT GSIL+CVPP +LIC+V+ LST 
Sbjct: 370 AENLLYCGGMILEFIAFVRLRKKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTI 429

Query: 435 KVMIVSLVAVAIGVMLHPGLKYVEKKRWLKFSVSSDLPDLHFANRDRTDTLV 487
           KV +VS V V IG ++ P L +++ K+W+KFSV SDL +    N D  ++L+
Sbjct: 430 KVALVSFVMVVIGFLMKPCLNHMDGKKWVKFSVCSDLAEFQKENLDCEESLL 481

BLAST of MC06g1716 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 548.1 bits (1411), Expect = 7.0e-156
Identity = 268/452 (59.29%), Postives = 340/452 (75.22%), Query Frame = 0

Query: 16  MGELNRVEYVSVGETPTPSAHNVKKLSVLPLVFLIFYEVSGGPFGVEDSV-GAAGPLLAL 75
           MGE   +        P PS     KL++LPLVFLIFYEVSGGPFGVEDSV    GPLLAL
Sbjct: 1   MGEEETIVNDENSSKPKPS----PKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLAL 60

Query: 76  LGFLVFPFIWSIPEALITAEMGTMFPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNA 135
           LGFL+FP IWSIPEAL+TAE+ T FPENGGYVVW+SSA GPFWGFQ+G+ KW SGV+DNA
Sbjct: 61  LGFLIFPLIWSIPEALVTAELATSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNA 120

Query: 136 LYPVLFLDYLESEIPALGGGFPRVAAVLALTVILTYMNYRGLTIVGWVAVVLGVFSILPF 195
           LYPVLFLDYL+   P L     RV A+L +T  LTY+NYRGL IVG+ AVVL VFS+ PF
Sbjct: 121 LYPVLFLDYLKHSFPVLDHVAARVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPF 180

Query: 196 AVMGLVSIPNLRPDGWLVVNLRDVDWNLYLNTLFWNLNYWDSISTLAGEVDNPNKTLPKG 255
            VM L+++PN+RP  WL V+ + ++W  Y NT+FWNLNYWD  STLAGEVD P KT PK 
Sbjct: 181 VVMALLAVPNIRPKRWLFVDTQKINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKA 240

Query: 256 LFYALILVVLGYFLPLLSGTGAIPLNRD-SWTDGYFSDVAKMIGGAWLSWWVQGAAAMSN 315
           LF A++LV+  Y +PL++GTGA+  +    W+DGYF++V  +IGG WL  W+Q AAAMSN
Sbjct: 241 LFGAVLLVMGSYLIPLMAGTGALSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSN 300

Query: 316 MGMFVAEMSSDSFQLLGMAERGMLPEFFSKRSRHGTPLIGILFSASGVVLLSWLSFQEIV 375
           +G+F AEMSSD+FQLLGM+E GMLP FF++RS++GTP I IL SA+GV+ LSW+SFQEI+
Sbjct: 301 LGLFEAEMSSDAFQLLGMSEIGMLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEII 360

Query: 376 AAENFLYCFGMILEFIAFVKLRIKHPAASRPYKIPVGTAGSILMCVPPTILICIVLALST 435
              NFLY  GM+LEF AFVKLRIK P   RPY++P+ T G  ++C+PP++L+ +V+ L+ 
Sbjct: 361 EFLNFLYALGMLLEFAAFVKLRIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAA 420

Query: 436 AKVMIVSLVAVAIGVMLHPGLKYVEKKRWLKF 466
            K  ++S V + +G  L+P L  V++K+W +F
Sbjct: 421 PKTFLISGVIIVLGFCLYPFLTLVKEKQWARF 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6S55.3e-21777.00Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
A2X8M89.1e-20173.83Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D09.1e-20173.83Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Q9FFL16.1e-19771.74Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
Q9C6S49.1e-19370.97Probable polyamine transporter At1g31820 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Match NameE-valueIdentityDescription
XP_022159490.10.0100.00probable polyamine transporter At1g31830 isoform X1 [Momordica charantia][more]
XP_022159491.10.0100.00probable polyamine transporter At1g31830 isoform X2 [Momordica charantia][more]
XP_038887706.10.091.58probable polyamine transporter At1g31830 isoform X1 [Benincasa hispida][more]
KAG6606291.10.090.95putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7036233.10.090.95putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
Match NameE-valueIdentityDescription
A0A6J1DYW60.0100.00probable polyamine transporter At1g31830 isoform X1 OS=Momordica charantia OX=36... [more]
A0A6J1E4330.0100.00probable polyamine transporter At1g31830 isoform X2 OS=Momordica charantia OX=36... [more]
A0A0A0LBS30.091.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G636430 PE=3 SV=1[more]
A0A1S4E2U00.091.17probable polyamine transporter At1g31830 isoform X5 OS=Cucumis melo OX=3656 GN=L... [more]
A0A1S3CCI90.091.17probable polyamine transporter At1g31830 isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G31830.13.8e-21877.00Amino acid permease family protein [more]
AT1G31830.23.8e-21877.00Amino acid permease family protein [more]
AT5G05630.14.3e-19871.74Amino acid permease family protein [more]
AT1G31820.16.5e-19470.97Amino acid permease family protein [more]
AT3G19553.17.0e-15659.29Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 37..468
e-value: 2.4E-61
score: 209.7
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 23..484
e-value: 2.3E-79
score: 264.6
NoneNo IPR availablePANTHERPTHR45826:SF10POLYAMINE TRANSPORTER PUT1coord: 9..478
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 42..444
e-value: 2.5E-39
score: 135.4
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 9..478

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1716.1MC06g1716.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity