MC06g1682 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1682
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionInositol-tetrakisphosphate 1-kinase
LocationMC06: 24223877 .. 24224959 (+)
RNA-Seq ExpressionMC06g1682
SyntenyMC06g1682
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAAGCCGAACGGAGATTCTGCGTCGGGTATGCCCTTGCGCCGAAGAAGCGACGTAGTTTCATACGAGACTCGCTGGTGATGCTTGCGGAATCGCGAGGTATCGATCTTGTGCGGATCGACACGGACCGGCCCCTTGTTGATCAAGGAGCCTTCGATTGCGTCATCCACAAGCTGTATGGTGCAGATTGGAGGAGGCAACTGGAGAATTTTAGGGTTGTGAACCCTAGTGCGGTGATTTTGGACTCACCGGACGCGATTCTGAGGCTTCACAACAGGATTTCCATGCTCCAGGTGGTATCGGAGCTGAAGGTTGAAAACCACGAGGAGTCGTTTGGGATTCCTAAGCAGATTGTGATTTACGATAAGGAGACTCTATCGGATCCGCAGGCTTGGGAGGCCTTGAAGTTCCCGGTCATTGCCAAGCCCTTGGTCGCCGATGGCAGTGCCAAATCGCATAAAATGGCGCTCGTGTTTAATCACGACGGCTTGAACATGCTTAAGCCTCCTATTGTATTGCAAGAATTTGTGAACCACGGCGGGGTTATATTCAAGGTTTATGTGGCCGGAGAGCACGTAAAATGCGTAAAGAGGAAGTCACTTCCGGACGTTTCTGAGGAGAAACTGGAGAGCGTCGAGGGCCTGCAGTCATTCTCTCAGGTGTCAAATTTGACCAACCACGAGAGAGTTGACGAGAAGTATTATCAGATGATGCAATTGGACGACACTGAGATGCCACCATTGAGTTTCGTCACTGATATAGCCAGAGGATTACGGCATGCGATGAAGCTTAATCTCTTCAACTTCGATATGATTCGGGATTCAATAAACGGGAATCGGTACCTCATAGTTGATATCAATTACTTTCCGGGGTTCGCCAAAATGCCAGGGTACGAGAAGATTTTAACGGATTTCCTCTCTGACGTAATGTGGAGGAAAGACAGAGAGGTGGTAAAAGCTTGGTTCGAGCACGATGGCTCAGGGCCGACTGCCTTCGATAAAAGGCAAGTCCACACTTGTGAAAATGATTCTAGAAAGGTTGTGATGCTTACTTGCATCAGTAATGGGGACGGAGGAGGT

mRNA sequence

ATGGCGGAAGCCGAACGGAGATTCTGCGTCGGGTATGCCCTTGCGCCGAAGAAGCGACGTAGTTTCATACGAGACTCGCTGGTGATGCTTGCGGAATCGCGAGGTATCGATCTTGTGCGGATCGACACGGACCGGCCCCTTGTTGATCAAGGAGCCTTCGATTGCGTCATCCACAAGCTGTATGGTGCAGATTGGAGGAGGCAACTGGAGAATTTTAGGGTTGTGAACCCTAGTGCGGTGATTTTGGACTCACCGGACGCGATTCTGAGGCTTCACAACAGGATTTCCATGCTCCAGGTGGTATCGGAGCTGAAGGTTGAAAACCACGAGGAGTCGTTTGGGATTCCTAAGCAGATTGTGATTTACGATAAGGAGACTCTATCGGATCCGCAGGCTTGGGAGGCCTTGAAGTTCCCGGTCATTGCCAAGCCCTTGGTCGCCGATGGCAGTGCCAAATCGCATAAAATGGCGCTCGTGTTTAATCACGACGGCTTGAACATGCTTAAGCCTCCTATTGTATTGCAAGAATTTGTGAACCACGGCGGGGTTATATTCAAGGTTTATGTGGCCGGAGAGCACGTAAAATGCGTAAAGAGGAAGTCACTTCCGGACGTTTCTGAGGAGAAACTGGAGAGCGTCGAGGGCCTGCAGTCATTCTCTCAGGTGTCAAATTTGACCAACCACGAGAGAGTTGACGAGAAGTATTATCAGATGATGCAATTGGACGACACTGAGATGCCACCATTGAGTTTCGTCACTGATATAGCCAGAGGATTACGGCATGCGATGAAGCTTAATCTCTTCAACTTCGATATGATTCGGGATTCAATAAACGGGAATCGGTACCTCATAGTTGATATCAATTACTTTCCGGGGTTCGCCAAAATGCCAGGGTACGAGAAGATTTTAACGGATTTCCTCTCTGACGTAATGTGGAGGAAAGACAGAGAGGTGGTAAAAGCTTGGTTCGAGCACGATGGCTCAGGGCCGACTGCCTTCGATAAAAGGCAAGTCCACACTTGTGAAAATGATTCTAGAAAGGTTGTGATGCTTACTTGCATCAGTAATGGGGACGGAGGAGGT

Coding sequence (CDS)

ATGGCGGAAGCCGAACGGAGATTCTGCGTCGGGTATGCCCTTGCGCCGAAGAAGCGACGTAGTTTCATACGAGACTCGCTGGTGATGCTTGCGGAATCGCGAGGTATCGATCTTGTGCGGATCGACACGGACCGGCCCCTTGTTGATCAAGGAGCCTTCGATTGCGTCATCCACAAGCTGTATGGTGCAGATTGGAGGAGGCAACTGGAGAATTTTAGGGTTGTGAACCCTAGTGCGGTGATTTTGGACTCACCGGACGCGATTCTGAGGCTTCACAACAGGATTTCCATGCTCCAGGTGGTATCGGAGCTGAAGGTTGAAAACCACGAGGAGTCGTTTGGGATTCCTAAGCAGATTGTGATTTACGATAAGGAGACTCTATCGGATCCGCAGGCTTGGGAGGCCTTGAAGTTCCCGGTCATTGCCAAGCCCTTGGTCGCCGATGGCAGTGCCAAATCGCATAAAATGGCGCTCGTGTTTAATCACGACGGCTTGAACATGCTTAAGCCTCCTATTGTATTGCAAGAATTTGTGAACCACGGCGGGGTTATATTCAAGGTTTATGTGGCCGGAGAGCACGTAAAATGCGTAAAGAGGAAGTCACTTCCGGACGTTTCTGAGGAGAAACTGGAGAGCGTCGAGGGCCTGCAGTCATTCTCTCAGGTGTCAAATTTGACCAACCACGAGAGAGTTGACGAGAAGTATTATCAGATGATGCAATTGGACGACACTGAGATGCCACCATTGAGTTTCGTCACTGATATAGCCAGAGGATTACGGCATGCGATGAAGCTTAATCTCTTCAACTTCGATATGATTCGGGATTCAATAAACGGGAATCGGTACCTCATAGTTGATATCAATTACTTTCCGGGGTTCGCCAAAATGCCAGGGTACGAGAAGATTTTAACGGATTTCCTCTCTGACGTAATGTGGAGGAAAGACAGAGAGGTGGTAAAAGCTTGGTTCGAGCACGATGGCTCAGGGCCGACTGCCTTCGATAAAAGGCAAGTCCACACTTGTGAAAATGATTCTAGAAAGGTTGTGATGCTTACTTGCATCAGTAATGGGGACGGAGGAGGT

Protein sequence

MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIVIYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHGGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQLDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYEKILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGGG
Homology
BLAST of MC06g1682 vs. ExPASy Swiss-Prot
Match: A0JJZ6 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 4.8e-106
Identity = 192/315 (60.95%), Postives = 241/315 (76.51%), Query Frame = 0

Query: 6   RRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGADW 65
           RR+ +GYALAPKK++SFI+ SLV  A  RG+DLV +D  RPL +QG F  +IHKLYG +W
Sbjct: 14  RRYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEW 73

Query: 66  RRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKV---ENHEESFGIPKQIVIY 125
           R QL+ F   +P+  ++D P AI RLHNRISMLQVVSEL V    +H  +FGIP Q+V+Y
Sbjct: 74  RGQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVY 133

Query: 126 DKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHGG 185
           D   LSD     AL+FP+IAKPLVADG+AKSHKM+LV++ +GL  L+PP+VLQEFVNHGG
Sbjct: 134 DAAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGG 193

Query: 186 VIFKVYVAGEHVKCVKRKSLPDVSEEKLE--SVEGLQSFSQVSNLTNHERVDEKYYQMMQ 245
           VIFKVYV G HV CVKR+SLPDVS + L+  S EG  SFSQVSNL N ER  ++YY  M+
Sbjct: 194 VIFKVYVVGAHVTCVKRRSLPDVSSDVLQDASAEGSLSFSQVSNLPN-ERTAQEYYDDMR 253

Query: 246 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 305
           L+D  MPP +F+ DIA  LR A+ L+LFNFDMIRD+  G+RYL++DINYFPG+AKMPGYE
Sbjct: 254 LEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGYE 313

Query: 306 KILTDFLSDVMWRKD 316
            +LTDF  +++ + D
Sbjct: 314 TVLTDFFWEMVHKDD 327

BLAST of MC06g1682 vs. ExPASy Swiss-Prot
Match: Q7XBW0 (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 4.8e-106
Identity = 192/315 (60.95%), Postives = 241/315 (76.51%), Query Frame = 0

Query: 6   RRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGADW 65
           RR+ +GYALAPKK++SFI+ SLV  A  RG+DLV +D  RPL +QG F  +IHKLYG +W
Sbjct: 14  RRYLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEW 73

Query: 66  RRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKV---ENHEESFGIPKQIVIY 125
           R QL+ F   +P+  ++D P AI RLHNRISMLQVVSEL V    +H  +FGIP Q+V+Y
Sbjct: 74  RGQLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVY 133

Query: 126 DKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHGG 185
           D   LSD     AL+FP+IAKPLVADG+AKSHKM+LV++ +GL  L+PP+VLQEFVNHGG
Sbjct: 134 DAAALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGG 193

Query: 186 VIFKVYVAGEHVKCVKRKSLPDVSEEKLE--SVEGLQSFSQVSNLTNHERVDEKYYQMMQ 245
           VIFKVYV G HV CVKR+SLPDVS + L+  S EG  SFSQVSNL N ER  ++YY  M+
Sbjct: 194 VIFKVYVVGAHVTCVKRRSLPDVSSDVLQDASAEGSLSFSQVSNLPN-ERTAQEYYDDMR 253

Query: 246 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 305
           L+D  MPP +F+ DIA  LR A+ L+LFNFDMIRD+  G+RYL++DINYFPG+AKMPGYE
Sbjct: 254 LEDAIMPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGYE 313

Query: 306 KILTDFLSDVMWRKD 316
            +LTDF  +++ + D
Sbjct: 314 TVLTDFFWEMVHKDD 327

BLAST of MC06g1682 vs. ExPASy Swiss-Prot
Match: Q84Y01 (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-101
Identity = 186/306 (60.78%), Postives = 230/306 (75.16%), Query Frame = 0

Query: 7   RFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGADWR 66
           R+ +GYALAPKK++SFI+ SLV  A SRG+DLV +D  +PL +QG F  +IHKLYG DWR
Sbjct: 18  RYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAEQGPFHLLIHKLYGDDWR 77

Query: 67  RQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSEL-KVENHEESFGIPKQIVIYDKE 126
            QL  F   +P+  I+D P AI RLHNRISMLQVVSEL    + + +FGIP Q+V+YD  
Sbjct: 78  AQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAADQDSTFGIPSQVVVYDAA 137

Query: 127 TLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHGGVIF 186
            L+D     AL+FP+IAKPLVADG+AKSHKM+LV++ +GL  L+PP+VLQEFVNHGGVIF
Sbjct: 138 ALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKLRPPLVLQEFVNHGGVIF 197

Query: 187 KVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQLDDTE 246
           KVYV G HV CVKR+SLPDVS E   S +G  SFSQVSNL   ER  E+YY    L+D  
Sbjct: 198 KVYVVGGHVTCVKRRSLPDVSPEDDASAQGSVSFSQVSNLPT-ERTAEEYYGEKSLEDAV 257

Query: 247 MPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYEKILTD 306
           +PP +F+  IA GLR A+ L LFNFDMIRD   G+RYL++DINYFPG+AKMPGYE +LTD
Sbjct: 258 VPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYLVIDINYFPGYAKMPGYETVLTD 317

Query: 307 FLSDVM 312
           F  +++
Sbjct: 318 FFWEMV 322

BLAST of MC06g1682 vs. ExPASy Swiss-Prot
Match: Q9SBA5 (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.0e-100
Identity = 175/312 (56.09%), Postives = 235/312 (75.32%), Query Frame = 0

Query: 5   ERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGAD 64
           + R+ VGYALA KK+ SFI+ SL+  +  RGIDLV++D  + L++QG  DC+IHKLY   
Sbjct: 6   QERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVY 65

Query: 65  WRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVE-NHEESFGIPKQIVIYD 124
           W+  L  FR   P   ++D P+AI RLHNR+SML+V+++L+   +  E FG+P+Q+V+ D
Sbjct: 66  WKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSERFGVPEQVVVMD 125

Query: 125 KETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHGGV 184
              LS   A   LKFPVIAKPL ADGSAKSHKM L+++ +G+ +LK PIVLQEFVNHGGV
Sbjct: 126 SSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGV 185

Query: 185 IFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQLDD 244
           IFKVYV G+HVKCVKR+SLPD+SEEK+ + +G   FSQ+SNLT  E  + +Y +   L+ 
Sbjct: 186 IFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEK 245

Query: 245 TEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYEKIL 304
            EMPPLSF+TD+A+ +R +M LNLFNFD+IRD+ + NRYLI+DINYFPG+AKMP YE +L
Sbjct: 246 VEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVL 305

Query: 305 TDFLSDVMWRKD 316
           T+F  D++ +K+
Sbjct: 306 TEFFWDMVTKKN 317

BLAST of MC06g1682 vs. ExPASy Swiss-Prot
Match: A2X4M8 (Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=ITPK4 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 2.7e-72
Identity = 151/312 (48.40%), Postives = 199/312 (63.78%), Query Frame = 0

Query: 7   RFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGADWR 66
           R+ VGYAL P+K  S +R SLV LA  RG+ LV +D  RPL +QG FD ++HK+Y   WR
Sbjct: 13  RYTVGYALLPEKVSSVVRPSLVALAADRGVRLVAVDVSRPLAEQGPFDLLVHKMYDRGWR 72

Query: 67  RQLENFRVVNPSA-VILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIVIYDKE 126
            QLE     +P   V++DSP AI RL +R +ML VVS L+         +P Q+V+ D  
Sbjct: 73  AQLEELAARHPGVPVVVDSPGAIDRLLDRATMLDVVSGLRT-----PVSVPPQVVVSDAA 132

Query: 127 TLSDP-QAWEALKFPVIAKPLVADGSAKSHKMALVFNHDG-LNMLKPPIVLQEFVNHGGV 186
             +D   A  AL+FP+IAKPL  DGSA+SH M LV+  DG L +L+ P+VLQEFVNHGGV
Sbjct: 133 ADADELLARAALRFPLIAKPLAVDGSAESHDMRLVYRRDGVLPLLRAPLVLQEFVNHGGV 192

Query: 187 IFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQS--FSQVSNLTNHERVDEKYYQMMQL 246
           +FKVYV G+   CV+R SLPDV   +L  ++   S  F+ +SN       D+        
Sbjct: 193 LFKVYVVGDRATCVRRSSLPDVPARRLLDLDAEPSVPFANISNQPLPPPDDDG-----GA 252

Query: 247 DDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRD--SINGNRYLIVDINYFPGFAKMPGY 306
            D + P   FV ++ARGLR  + L+LFNFDMIR+    +G+RY I+DINYFPG+AKMPGY
Sbjct: 253 ADDDTPAAGFVDEVARGLRRGLGLHLFNFDMIRERSEEHGDRYFIIDINYFPGYAKMPGY 312

Query: 307 EKILTDFLSDVM 312
           E  LTDF  +++
Sbjct: 313 EAALTDFFLEML 314

BLAST of MC06g1682 vs. NCBI nr
Match: XP_022131251.1 (inositol-tetrakisphosphate 1-kinase 1-like [Momordica charantia])

HSP 1 Score: 731 bits (1886), Expect = 5.27e-266
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL
Sbjct: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV
Sbjct: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH
Sbjct: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG
Sbjct: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. NCBI nr
Match: XP_023553896.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650 bits (1677), Expect = 3.77e-234
Identity = 321/361 (88.92%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAE  RRFCVGYAL PKKRRSFIRDSLV LAESRGIDLVRIDTDRPLVDQGAFDCV+HKL
Sbjct: 1   MAEVGRRFCVGYALMPKKRRSFIRDSLVRLAESRGIDLVRIDTDRPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENF+VVNP+AVILDS DAI RLHNRISMLQVVSELK+ENH+ESFGIP+QI 
Sbjct: 61  YGADWRRQLENFKVVNPNAVILDSLDAIERLHNRISMLQVVSELKIENHDESFGIPEQIA 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKE LSD +AWEALKFPVIAKPLVADGSAKSHKMALVFNHD LN +KPPIVLQEFVNH
Sbjct: 121 IYDKEKLSDRRAWEALKFPVIAKPLVADGSAKSHKMALVFNHDALNKVKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAG+HVKCVKRKSLPDVSEEK +SVEGL SFSQVSNLTNHERVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGQHVKCVKRKSLPDVSEEKSDSVEGLLSFSQVSNLTNHERVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIA+GLRHAMKLNLFNFD++RDS N NRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIAKGLRHAMKLNLFNFDVMRDSNNRNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDF SD+M  KDRE+VKAWFE+D S P AFDKRQV+ CENDSR VVMLTCISNGDGG
Sbjct: 301 KILTDFFSDIMRMKDRELVKAWFENDASEPPAFDKRQVNNCENDSRNVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. NCBI nr
Match: KAG7011380.1 (Inositol-tetrakisphosphate 1-kinase 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 648 bits (1671), Expect = 3.09e-233
Identity = 320/361 (88.64%), Postives = 337/361 (93.35%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAE  RRFCVGYAL PKKRRSFIRDSLV LAESRGIDLVRIDTDRPLVDQGAFDCV+HKL
Sbjct: 1   MAEVGRRFCVGYALMPKKRRSFIRDSLVRLAESRGIDLVRIDTDRPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENF+VVNP+AVILDS DAI RLHNRISMLQVVSELK+ENH+ESFGIP+QI 
Sbjct: 61  YGADWRRQLENFKVVNPNAVILDSLDAIERLHNRISMLQVVSELKIENHDESFGIPEQIA 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKE LSD +AWEALKFPVIAKPLVADGSAKSHKMALVFNHD LN +KPPIVLQEFVNH
Sbjct: 121 IYDKEKLSDRRAWEALKFPVIAKPLVADGSAKSHKMALVFNHDALNKVKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAG+HVKCVKRKSLPDVSEEK +SVEGL SFSQVSNLTNHERVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGQHVKCVKRKSLPDVSEEKSDSVEGLLSFSQVSNLTNHERVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIA+GLRHAMKLNLFNFD++RDS N NRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIAKGLRHAMKLNLFNFDVMRDSNNRNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDF SD+M  KD E+VKAWFE+D S P AFDKRQV+ CENDSR VVMLTCISNGDGG
Sbjct: 301 KILTDFFSDIMRMKDSELVKAWFENDASEPPAFDKRQVNNCENDSRNVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. NCBI nr
Match: XP_022967283.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima])

HSP 1 Score: 647 bits (1670), Expect = 4.39e-233
Identity = 319/361 (88.37%), Postives = 337/361 (93.35%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           M E  RRFCVGYAL PKKRRSFIRDSLV LAESRGIDLVRIDTDRPLVDQGAFDCV+HKL
Sbjct: 1   MTEVGRRFCVGYALMPKKRRSFIRDSLVRLAESRGIDLVRIDTDRPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENF+VVNP+AVILDS DAI RLHNRISMLQVVSELK+ENH+ESFGIP+QI 
Sbjct: 61  YGADWRRQLENFKVVNPNAVILDSLDAIERLHNRISMLQVVSELKIENHDESFGIPEQIA 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKE LSD +AWEALKFPVIAKPLVADGSAKSHKMALVF HD LN +KPPIVLQEFVNH
Sbjct: 121 IYDKEKLSDRRAWEALKFPVIAKPLVADGSAKSHKMALVFKHDALNKVKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAG+HVKCVKRKSLPDVSEEKL+SVEGL SFSQVSNLTNH+RVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGQHVKCVKRKSLPDVSEEKLDSVEGLLSFSQVSNLTNHDRVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIA+GLRHAMKLNLFNFD++RDS N NRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIAKGLRHAMKLNLFNFDVMRDSRNRNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDF SD+M  KDRE+VKAWFE+D S P AFDKRQV+ CENDSR VVMLTCISNGDGG
Sbjct: 301 KILTDFFSDIMRMKDRELVKAWFENDASEPPAFDKRQVNNCENDSRNVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. NCBI nr
Match: XP_022963555.1 (inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata])

HSP 1 Score: 645 bits (1665), Expect = 2.54e-232
Identity = 319/361 (88.37%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAE  RRFCVGYAL PKKRRSFIRDSLV LAESRGIDLVRIDTDRPLVDQGAFDCV+HKL
Sbjct: 1   MAEVGRRFCVGYALMPKKRRSFIRDSLVRLAESRGIDLVRIDTDRPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENF+VVNP+AVILDS DAI RLHNRISMLQVVSELK+ENH+ESFGIP+QI 
Sbjct: 61  YGADWRRQLENFKVVNPNAVILDSLDAIERLHNRISMLQVVSELKIENHDESFGIPEQIA 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKE LSD +AWEALKFPVIAKPLVADGSAKSHKMALVFNHD LN +KPPIVLQEFVNH
Sbjct: 121 IYDKEKLSDRRAWEALKFPVIAKPLVADGSAKSHKMALVFNHDALNKVKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAG+HVKCVKRKSLPDVSEEK +SVEGL SFSQVSNLTNHERVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGQHVKCVKRKSLPDVSEEKSDSVEGLLSFSQVSNLTNHERVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIA+GLRHAMKLNLFNFD++RDS N NRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIAKGLRHAMKLNLFNFDVMRDSNNRNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDF SD+M  KD E+VKAWFE+D S P AFDKRQV+ C NDSR VVMLTCISNGDGG
Sbjct: 301 KILTDFFSDIMRMKDSELVKAWFENDASEPPAFDKRQVNNCANDSRNVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. ExPASy TrEMBL
Match: A0A6J1BP17 (Inositol-tetrakisphosphate 1-kinase OS=Momordica charantia OX=3673 GN=LOC111004509 PE=3 SV=1)

HSP 1 Score: 731 bits (1886), Expect = 2.55e-266
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL
Sbjct: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV
Sbjct: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH
Sbjct: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG
Sbjct: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. ExPASy TrEMBL
Match: A0A6J1HUM6 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111466852 PE=3 SV=1)

HSP 1 Score: 647 bits (1670), Expect = 2.13e-233
Identity = 319/361 (88.37%), Postives = 337/361 (93.35%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           M E  RRFCVGYAL PKKRRSFIRDSLV LAESRGIDLVRIDTDRPLVDQGAFDCV+HKL
Sbjct: 1   MTEVGRRFCVGYALMPKKRRSFIRDSLVRLAESRGIDLVRIDTDRPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENF+VVNP+AVILDS DAI RLHNRISMLQVVSELK+ENH+ESFGIP+QI 
Sbjct: 61  YGADWRRQLENFKVVNPNAVILDSLDAIERLHNRISMLQVVSELKIENHDESFGIPEQIA 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKE LSD +AWEALKFPVIAKPLVADGSAKSHKMALVF HD LN +KPPIVLQEFVNH
Sbjct: 121 IYDKEKLSDRRAWEALKFPVIAKPLVADGSAKSHKMALVFKHDALNKVKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAG+HVKCVKRKSLPDVSEEKL+SVEGL SFSQVSNLTNH+RVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGQHVKCVKRKSLPDVSEEKLDSVEGLLSFSQVSNLTNHDRVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIA+GLRHAMKLNLFNFD++RDS N NRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIAKGLRHAMKLNLFNFDVMRDSRNRNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDF SD+M  KDRE+VKAWFE+D S P AFDKRQV+ CENDSR VVMLTCISNGDGG
Sbjct: 301 KILTDFFSDIMRMKDRELVKAWFENDASEPPAFDKRQVNNCENDSRNVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. ExPASy TrEMBL
Match: A0A6J1HIB8 (Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC111463848 PE=3 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 1.23e-232
Identity = 319/361 (88.37%), Postives = 336/361 (93.07%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAE  RRFCVGYAL PKKRRSFIRDSLV LAESRGIDLVRIDTDRPLVDQGAFDCV+HKL
Sbjct: 1   MAEVGRRFCVGYALMPKKRRSFIRDSLVRLAESRGIDLVRIDTDRPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           YGADWRRQLENF+VVNP+AVILDS DAI RLHNRISMLQVVSELK+ENH+ESFGIP+QI 
Sbjct: 61  YGADWRRQLENFKVVNPNAVILDSLDAIERLHNRISMLQVVSELKIENHDESFGIPEQIA 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYDKE LSD +AWEALKFPVIAKPLVADGSAKSHKMALVFNHD LN +KPPIVLQEFVNH
Sbjct: 121 IYDKEKLSDRRAWEALKFPVIAKPLVADGSAKSHKMALVFNHDALNKVKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAG+HVKCVKRKSLPDVSEEK +SVEGL SFSQVSNLTNHERVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGQHVKCVKRKSLPDVSEEKSDSVEGLLSFSQVSNLTNHERVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIA+GLRHAMKLNLFNFD++RDS N NRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIAKGLRHAMKLNLFNFDVMRDSNNRNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KILTDF SD+M  KD E+VKAWFE+D S P AFDKRQV+ C NDSR VVMLTCISNGDGG
Sbjct: 301 KILTDFFSDIMRMKDSELVKAWFENDASEPPAFDKRQVNNCANDSRNVVMLTCISNGDGG 360

BLAST of MC06g1682 vs. ExPASy TrEMBL
Match: A0A5D3E5R5 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004630 PE=3 SV=1)

HSP 1 Score: 645 bits (1664), Expect = 1.75e-232
Identity = 313/361 (86.70%), Postives = 335/361 (92.80%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAE  RRFC+GYAL PKKRRSFIR+SL+ LAES+GID VRID D+PLVDQGAFDCV+HKL
Sbjct: 1   MAEIGRRFCIGYALLPKKRRSFIRESLLRLAESKGIDFVRIDMDKPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIV 120
           Y ADWR+QLENFRV+NP+ VILDS DAI RLHNRISMLQVVSELK+ENH+ESFGIP+QIV
Sbjct: 61  YSADWRKQLENFRVLNPNVVILDSLDAIERLHNRISMLQVVSELKIENHDESFGIPEQIV 120

Query: 121 IYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNH 180
           IYD E LSD +AWE LKFPV+AKPLVADGSAKSHKMALVFNHDGLN LKPPIVLQEFVNH
Sbjct: 121 IYDMENLSDRRAWETLKFPVVAKPLVADGSAKSHKMALVFNHDGLNKLKPPIVLQEFVNH 180

Query: 181 GGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQ 240
           GGVIFKVYVAG HVKCVKRKSLPD+SEEKLESVE LQSFSQVSNLTNH+RVDEKYYQMMQ
Sbjct: 181 GGVIFKVYVAGNHVKCVKRKSLPDISEEKLESVEDLQSFSQVSNLTNHDRVDEKYYQMMQ 240

Query: 241 LDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYE 300
           LDDTEMPPLSFVTDIA+GLRHA+KLNLFNFDM+RDS N NRYLIVDINYFPGFAKMPGYE
Sbjct: 241 LDDTEMPPLSFVTDIAKGLRHALKLNLFNFDMMRDSRNKNRYLIVDINYFPGFAKMPGYE 300

Query: 301 KILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDGG 360
           KI+TDFLSD+M RKDRE+VKAWFEHD S P AFDKRQVH CENDSR VVMLTCI NGDGG
Sbjct: 301 KIVTDFLSDIMRRKDRELVKAWFEHDASDPIAFDKRQVHNCENDSRNVVMLTCIGNGDGG 360

BLAST of MC06g1682 vs. ExPASy TrEMBL
Match: A0A0A0LK17 (Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus OX=3659 GN=Csa_3G901120 PE=3 SV=1)

HSP 1 Score: 639 bits (1647), Expect = 7.05e-230
Identity = 312/362 (86.19%), Postives = 334/362 (92.27%), Query Frame = 0

Query: 1   MAEAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKL 60
           MAE  RRFC+GYAL PKKRRSFIRDSL+ LAES+GID VRID D+PLVDQGAFDCV+HKL
Sbjct: 1   MAEIGRRFCIGYALLPKKRRSFIRDSLLRLAESKGIDFVRIDMDKPLVDQGAFDCVLHKL 60

Query: 61  YGADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVEN-HEESFGIPKQI 120
           Y ADWR+QLENFR +NP+ VILDS DAI RLHNRISMLQVVSELK+EN H+ESFGIP+QI
Sbjct: 61  YTADWRKQLENFRTINPNVVILDSLDAIERLHNRISMLQVVSELKIENNHDESFGIPEQI 120

Query: 121 VIYDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVN 180
           VIYDKE LSD +AWE LKFPVIAKP+VADGSAKSHKMALVFNHDGLN LKPP+VLQEFVN
Sbjct: 121 VIYDKEDLSDRRAWETLKFPVIAKPVVADGSAKSHKMALVFNHDGLNKLKPPLVLQEFVN 180

Query: 181 HGGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMM 240
           HGGVIFKVYVAG HVKCVKRKSLPD+SE+ LESVE LQSFSQVSNLTNHERVDEKYYQMM
Sbjct: 181 HGGVIFKVYVAGNHVKCVKRKSLPDISEDTLESVEDLQSFSQVSNLTNHERVDEKYYQMM 240

Query: 241 QLDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGY 300
           QLDDTEMPPLSFVTDIA+GLRHA+KLNLFNFDM+RDS N NRYLIVDINYFPGFAKMPGY
Sbjct: 241 QLDDTEMPPLSFVTDIAKGLRHALKLNLFNFDMMRDSRNKNRYLIVDINYFPGFAKMPGY 300

Query: 301 EKILTDFLSDVMWRKDREVVKAWFEHDGSGPTAFDKRQVHTCENDSRKVVMLTCISNGDG 360
           EKI+TDFLSD+M R DRE+VKAWFEHD S P AFDKRQVH CENDSRKVVMLTCI NGDG
Sbjct: 301 EKIVTDFLSDIMRRNDRELVKAWFEHDASDPIAFDKRQVHNCENDSRKVVMLTCIGNGDG 360

BLAST of MC06g1682 vs. TAIR 10
Match: AT5G16760.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 366.7 bits (940), Expect = 2.1e-101
Identity = 175/312 (56.09%), Postives = 235/312 (75.32%), Query Frame = 0

Query: 5   ERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGAD 64
           + R+ VGYALA KK+ SFI+ SL+  +  RGIDLV++D  + L++QG  DC+IHKLY   
Sbjct: 6   QERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKLYDVY 65

Query: 65  WRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVE-NHEESFGIPKQIVIYD 124
           W+  L  FR   P   ++D P+AI RLHNR+SML+V+++L+   +  E FG+P+Q+V+ D
Sbjct: 66  WKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSERFGVPEQVVVMD 125

Query: 125 KETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHGGV 184
              LS   A   LKFPVIAKPL ADGSAKSHKM L+++ +G+ +LK PIVLQEFVNHGGV
Sbjct: 126 SSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFVNHGGV 185

Query: 185 IFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNLTNHERVDEKYYQMMQLDD 244
           IFKVYV G+HVKCVKR+SLPD+SEEK+ + +G   FSQ+SNLT  E  + +Y +   L+ 
Sbjct: 186 IFKVYVVGDHVKCVKRRSLPDISEEKIGTSKGSLPFSQISNLTAQEDKNIEYGEDRSLEK 245

Query: 245 TEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYEKIL 304
            EMPPLSF+TD+A+ +R +M LNLFNFD+IRD+ + NRYLI+DINYFPG+AKMP YE +L
Sbjct: 246 VEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGYAKMPSYEPVL 305

Query: 305 TDFLSDVMWRKD 316
           T+F  D++ +K+
Sbjct: 306 TEFFWDMVTKKN 317

BLAST of MC06g1682 vs. TAIR 10
Match: AT4G08170.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 272.7 bits (696), Expect = 4.2e-73
Identity = 142/310 (45.81%), Postives = 201/310 (64.84%), Query Frame = 0

Query: 5   ERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYGAD 64
           ++   VGYAL  KK +SF++  L  LA ++GI  V ID ++PL +QG FD V+HK  G +
Sbjct: 38  KKSIIVGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIGKE 97

Query: 65  WRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIVIYDK 124
           WRR LE FR+ +P   +LD PDAIL L NR SMLQ V+++ + +     G+PKQ+VI  K
Sbjct: 98  WRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVI-KK 157

Query: 125 ETLSDPQAWE--ALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHGG 184
           +  S P+A     L+ P++AKPLVADGSAKSH+++L ++   L  L+PP+VLQEFVNHGG
Sbjct: 158 DASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGG 217

Query: 185 VIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVS-NLTNHERVDEKYYQMMQL 244
           V+FKVY+ GE ++ V+R SLPDVS  +L    G+  F +VS    + +  D      +  
Sbjct: 218 VLFKVYIVGEAIRVVRRFSLPDVSRRELPKSAGVFRFPRVSCAAASADDAD------LDP 277

Query: 245 DDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPGYEK 304
              E+PP   +  +A+ LR  + L LFN D+IR+    +R+ ++DINYFPG+ KMP YE 
Sbjct: 278 SIAELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPEYEH 337

Query: 305 ILTDFLSDVM 312
           + TDFL  V+
Sbjct: 338 VFTDFLLSVV 340

BLAST of MC06g1682 vs. TAIR 10
Match: AT4G33770.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 256.1 bits (653), Expect = 4.1e-68
Identity = 131/319 (41.07%), Postives = 204/319 (63.95%), Query Frame = 0

Query: 3   EAERRFCVGYALAPKKRRSFIRDSLVMLAESRGIDLVRIDTDRPLVDQGAFDCVIHKLYG 62
           + +++  VGYAL  KK++SF++  L +LA  +GI  V ID +RPL +QG FD V+HKL G
Sbjct: 76  QQQQKLVVGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLG 135

Query: 63  ADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQ-IVI 122
            +W   +E+++  +P   +LD P +I R++NR SMLQ +++LK+ +   S  +PKQ +V+
Sbjct: 136 KEWEEVIEDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVL 195

Query: 123 YDKETLSDPQAWEALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVNHG 182
            D    +D      LKFP++AKPL  DG+AKSH++ L ++   L  L PP+VLQEFVNHG
Sbjct: 196 KDSAASADAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHG 255

Query: 183 GVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVSNL---TNHERVDEKYYQM 242
           GV+FKV+V G+ +K ++R SLP+VS  +   V+G+  F +VS+     ++  +D +    
Sbjct: 256 GVMFKVFVVGDVIKVMRRFSLPNVSNCEKAKVDGVFQFPRVSSAAASADNADLDPRV--- 315

Query: 243 MQLDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPG 302
                 E+PP  F+  + + LR  + L LFN DMIR+  + N + ++DINYFPG+ K+P 
Sbjct: 316 -----AELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPD 375

Query: 303 YEKILTDF---LSDVMWRK 315
           YE++  DF   L+ V ++K
Sbjct: 376 YEQVFVDFFQNLAQVKYKK 386

BLAST of MC06g1682 vs. TAIR 10
Match: AT4G33770.2 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.8e-60
Identity = 119/288 (41.32%), Postives = 183/288 (63.54%), Query Frame = 0

Query: 34  RGIDLVRIDTDRPLVDQGAFDCVIHKLYGADWRRQLENFRVVNPSAVILDSPDAILRLHN 93
           +GI  V ID +RPL +QG FD V+HKL G +W   +E+++  +P   +LD P +I R++N
Sbjct: 14  KGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVIEDYQQKHPEVTVLDPPGSIQRIYN 73

Query: 94  RISMLQVVSELKVENHEESFGIPKQ-IVIYDKETLSDPQAWEALKFPVIAKPLVADGSAK 153
           R SMLQ +++LK+ +   S  +PKQ +V+ D    +D      LKFP++AKPL  DG+AK
Sbjct: 74  RQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSAASADAVVEAGLKFPLVAKPLWIDGTAK 133

Query: 154 SHKMALVFNHDGLNMLKPPIVLQEFVNHGGVIFKVYVAGEHVKCVKRKSLPDVSEEKLES 213
           SH++ L ++   L  L PP+VLQEFVNHGGV+FKV+V G+ +K ++R SLP+VS  +   
Sbjct: 134 SHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMFKVFVVGDVIKVMRRFSLPNVSNCEKAK 193

Query: 214 VEGLQSFSQVSNL---TNHERVDEKYYQMMQLDDTEMPPLSFVTDIARGLRHAMKLNLFN 273
           V+G+  F +VS+     ++  +D +          E+PP  F+  + + LR  + L LFN
Sbjct: 194 VDGVFQFPRVSSAAASADNADLDPRV--------AELPPKPFLEALVKELRSLLGLRLFN 253

Query: 274 FDMIRDSINGNRYLIVDINYFPGFAKMPGYEKILTDF---LSDVMWRK 315
            DMIR+  + N + ++DINYFPG+ K+P YE++  DF   L+ V ++K
Sbjct: 254 IDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVFVDFFQNLAQVKYKK 293

BLAST of MC06g1682 vs. TAIR 10
Match: AT4G08170.1 (Inositol 1,3,4-trisphosphate 5/6-kinase family protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.9e-58
Identity = 116/253 (45.85%), Postives = 164/253 (64.82%), Query Frame = 0

Query: 62  GADWRRQLENFRVVNPSAVILDSPDAILRLHNRISMLQVVSELKVENHEESFGIPKQIVI 121
           G +WRR LE FR+ +P   +LD PDAIL L NR SMLQ V+++ + +     G+PKQ+VI
Sbjct: 7   GKEWRRILEEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVI 66

Query: 122 YDKETLSDPQAWE--ALKFPVIAKPLVADGSAKSHKMALVFNHDGLNMLKPPIVLQEFVN 181
             K+  S P+A     L+ P++AKPLVADGSAKSH+++L ++   L  L+PP+VLQEFVN
Sbjct: 67  -KKDASSIPEAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVN 126

Query: 182 HGGVIFKVYVAGEHVKCVKRKSLPDVSEEKLESVEGLQSFSQVS-NLTNHERVDEKYYQM 241
           HGGV+FKVY+ GE ++ V+R SLPDVS  +L    G+  F +VS    + +  D      
Sbjct: 127 HGGVLFKVYIVGEAIRVVRRFSLPDVSRRELPKSAGVFRFPRVSCAAASADDAD------ 186

Query: 242 MQLDDTEMPPLSFVTDIARGLRHAMKLNLFNFDMIRDSINGNRYLIVDINYFPGFAKMPG 301
           +     E+PP   +  +A+ LR  + L LFN D+IR+    +R+ ++DINYFPG+ KMP 
Sbjct: 187 LDPSIAELPPRPLLERLAKELRRGLGLRLFNLDIIREHGTRDRFYVIDINYFPGYGKMPE 246

Query: 302 YEKILTDFLSDVM 312
           YE + TDFL  V+
Sbjct: 247 YEHVFTDFLLSVV 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0JJZ64.8e-10660.95Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Q7XBW04.8e-10660.95Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q84Y012.1e-10160.78Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays OX=4577 GN=ITPK1 PE=2 SV=1[more]
Q9SBA53.0e-10056.09Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=ITPK1 P... [more]
A2X4M82.7e-7248.40Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. indica OX=39946 GN=... [more]
Match NameE-valueIdentityDescription
XP_022131251.15.27e-266100.00inositol-tetrakisphosphate 1-kinase 1-like [Momordica charantia][more]
XP_023553896.13.77e-23488.92inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita pepo subsp. pepo][more]
KAG7011380.13.09e-23388.64Inositol-tetrakisphosphate 1-kinase 5, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022967283.14.39e-23388.37inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita maxima][more]
XP_022963555.12.54e-23288.37inositol-tetrakisphosphate 1-kinase 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1BP172.55e-266100.00Inositol-tetrakisphosphate 1-kinase OS=Momordica charantia OX=3673 GN=LOC1110045... [more]
A0A6J1HUM62.13e-23388.37Inositol-tetrakisphosphate 1-kinase OS=Cucurbita maxima OX=3661 GN=LOC111466852 ... [more]
A0A6J1HIB81.23e-23288.37Inositol-tetrakisphosphate 1-kinase OS=Cucurbita moschata OX=3662 GN=LOC11146384... [more]
A0A5D3E5R51.75e-23286.70Inositol-tetrakisphosphate 1-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A0A0LK177.05e-23086.19Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus OX=3659 GN=Csa_3G901120 P... [more]
Match NameE-valueIdentityDescription
AT5G16760.12.1e-10156.09Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.24.2e-7345.81Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.14.1e-6841.07Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G33770.21.8e-6041.32Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
AT4G08170.12.9e-5845.85Inositol 1,3,4-trisphosphate 5/6-kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.470.20coord: 86..292
e-value: 1.5E-63
score: 216.3
NoneNo IPR availablePANTHERPTHR14217:SF17INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 3..327
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 83..294
IPR008656Inositol-tetrakisphosphate 1-kinasePIRSFPIRSF038186ITPKcoord: 1..321
e-value: 7.7E-126
score: 417.4
IPR008656Inositol-tetrakisphosphate 1-kinasePANTHERPTHR14217INOSITOL-TETRAKISPHOSPHATE 1-KINASEcoord: 3..327
IPR040464Inositol 1,3,4-trisphosphate 5/6-kinase, ATP-grasp domainPFAMPF05770Ins134_P3_kincoord: 108..308
e-value: 1.1E-83
score: 279.7
IPR041429Inositol-tetrakisphosphate 1-kinase, N-terminalPFAMPF17927Ins134_P3_kin_Ncoord: 10..88
e-value: 7.6E-28
score: 96.7
IPR011761ATP-grasp foldPROSITEPS50975ATP_GRASPcoord: 105..316
score: 8.657783

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1682.1MC06g1682.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0052746 inositol phosphorylation
biological_process GO:0032957 inositol trisphosphate metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function GO:0047325 inositol tetrakisphosphate 1-kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding