MC06g1673 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1673
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein TORNADO 2
LocationMC06: 24176197 .. 24178255 (-)
RNA-Seq ExpressionMC06g1673
SyntenyMC06g1673
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTAGCTAGCATACACTAAGTCCCAGCTAGCAGTGGTAACAGAAGAGATAAAACAAAAAAGGTAAACATTTACCACTCCTCCATCTTCTTCTACACTCCCTCACCAAATCCAAACAAAATATGGCACTCAACAAAAACATAATCGGCGGCATAAACTTGGTGGCGATGCTTCTTTCGGTGCCCATCATCGGCGCCGGAATCTGGCTGGCGACCCAGCCGGACAACGCCTGCCTCCAGATTCTGCAATGGCCACTCATCATTTTTGGCGTGGCGGTGCTCCTGGTGGGCGTGGCGGGCTTCATAGGAGCCTTCTGCCGGATCACCTGGCTCCTCCTCCTCTACCTGGTCGCAATGCTCGTCCTCATACTGCTGCTCGGTTCCCTCGTGGGGTTCGTGTACATGGTCACCCTCAGAGGCTCCGGCCATCTCCAGCCCAATAGGGCTTACTTGGAGTATCACCTGGACGACTTCTCCGCCTTCCTCCGCCGCAGAGTTCAGAGCTCCTTCAAGTGGGACCTCATCCGAACCTGCTTGAGCTCCTCCACCATGTGTGCTGAGTTGAACCAGAGCTTCCGCATGGCTCAGGATTTTTTCAATGCCCCCATTACCCCTATGCAGGTATATCCAAAACCCAAATTTTTCTCTTCATCGTTTCTTCTAAATCCTCCCCAACATTATATTCTCTCTTCTCTGACCTTTTGGATTTGTGTCGGCTATCTGATCCTGTATACTTTACAAACTTCTAATGACTTGGTTCATTAACTTCTAATTTTATAATACGACGTTTATCTTAACATTTACCAACTATTTTAAAGATTAGCCACGAGTTGATTGCTAGGCTTGTGTTTATGTGTTAAGCATATATAGCGACTCAGGACAATGTAAAAGATAAAAGAAAAATAAATACTTAGGCTAAATAAATACTTAGCTTTTCTCACAGGTGAGTACCAAATTTGTTCTACCCATCATTAAACTTTTAAATAGAAATGCAAAAATAGTATTCTTTTAAAAACTAATTAATGGTTAAATTTTTAATGTGGAGAGGACAAAATTGGTTCTCATTCGTGGGAAGTAAAATAATTTTCAAAAGATACTTATATGCATGTTTGTTTTAGGTGTTTATCAATTCGTAGGTATTTTCCTACTTAACACCTATTTAATTATTCTCAATATCTATAGAAGTTCATCTCAAAATCAATTTACAATTAGAGTAGTAACCTATTATATAATCTTAACATGTCTAAAGATAGTGACAATTTTAGGCTTAGCAAGATGTTCGTTCTAGTTCATACCATCTTCATATTTATAGGATTTCATCTTAAAATCAATTGGTAATGAGAGGATCAGCAACTTATGTATATTATTTGAATGATAATAGTATATAGTCGATGCGAATTATATCTCTATGGATCATATAAGACTTTCAGGATGATTCTAAAAGCAAACCTATGAGGATTTATAATAAAAAATATATATATTTTCTCTCTGTTCTTTTATGCATCATCTTGTGAGCTCTCGATTTTTTTCTACCATGAATAAGTATCTAAATATTATTGTTATTTAACAGTCAGGATGCTGCAAGCCGCCGACGTTGTGCGGGTACACATTTGTGAACCCAACGTACTGGATAATGCCCATAAACAATGCGGCGGACATGGACTGCCTCAAATGGAACAACGACCAAACCCAGCTCTGCTACAGCTGCGACTCCTGCAAGGCCGGCCTACTCCAGAGCTTGAAGAATCAATGGAGGACGGCTGATATAGTGCTCCTCTTGGCCCTCGTCGCCCTCATTTCTGTCTACATAATTGCCTGCTGCGCCTTTAGGAATGCCAAAACTGAGACACTCTTTGACAAATACAAGCAAGGCCAACCCACCTATGTGTAGCTTTTTCTTTTCTTTTTGTGTGTTGGTTTTGTTTCAAACGCTTTAATTCATACCACAATGGGGATATTTCAAATGATGTTTGTTTGACAAGATTTGAAAATGGAATTAGAATGATCAAGGCCTCAACTTAAGTCTGTTGGTAGTTTTAATATCTTTGGATGCACGAGAG

mRNA sequence

TTATTAGCTAGCATACACTAAGTCCCAGCTAGCAGTGGTAACAGAAGAGATAAAACAAAAAAGGTAAACATTTACCACTCCTCCATCTTCTTCTACACTCCCTCACCAAATCCAAACAAAATATGGCACTCAACAAAAACATAATCGGCGGCATAAACTTGGTGGCGATGCTTCTTTCGGTGCCCATCATCGGCGCCGGAATCTGGCTGGCGACCCAGCCGGACAACGCCTGCCTCCAGATTCTGCAATGGCCACTCATCATTTTTGGCGTGGCGGTGCTCCTGGTGGGCGTGGCGGGCTTCATAGGAGCCTTCTGCCGGATCACCTGGCTCCTCCTCCTCTACCTGGTCGCAATGCTCGTCCTCATACTGCTGCTCGGTTCCCTCGTGGGGTTCGTGTACATGGTCACCCTCAGAGGCTCCGGCCATCTCCAGCCCAATAGGGCTTACTTGGAGTATCACCTGGACGACTTCTCCGCCTTCCTCCGCCGCAGAGTTCAGAGCTCCTTCAAGTGGGACCTCATCCGAACCTGCTTGAGCTCCTCCACCATGTGTGCTGAGTTGAACCAGAGCTTCCGCATGGCTCAGGATTTTTTCAATGCCCCCATTACCCCTATGCAGTCAGGATGCTGCAAGCCGCCGACGTTGTGCGGGTACACATTTGTGAACCCAACGTACTGGATAATGCCCATAAACAATGCGGCGGACATGGACTGCCTCAAATGGAACAACGACCAAACCCAGCTCTGCTACAGCTGCGACTCCTGCAAGGCCGGCCTACTCCAGAGCTTGAAGAATCAATGGAGGACGGCTGATATAGTGCTCCTCTTGGCCCTCGTCGCCCTCATTTCTGTCTACATAATTGCCTGCTGCGCCTTTAGGAATGCCAAAACTGAGACACTCTTTGACAAATACAAGCAAGGCCAACCCACCTATGTGTAGCTTTTTCTTTTCTTTTTGTGTGTTGGTTTTGTTTCAAACGCTTTAATTCATACCACAATGGGGATATTTCAAATGATGTTTGTTTGACAAGATTTGAAAATGGAATTAGAATGATCAAGGCCTCAACTTAAGTCTGTTGGTAGTTTTAATATCTTTGGATGCACGAGAG

Coding sequence (CDS)

ATGGCACTCAACAAAAACATAATCGGCGGCATAAACTTGGTGGCGATGCTTCTTTCGGTGCCCATCATCGGCGCCGGAATCTGGCTGGCGACCCAGCCGGACAACGCCTGCCTCCAGATTCTGCAATGGCCACTCATCATTTTTGGCGTGGCGGTGCTCCTGGTGGGCGTGGCGGGCTTCATAGGAGCCTTCTGCCGGATCACCTGGCTCCTCCTCCTCTACCTGGTCGCAATGCTCGTCCTCATACTGCTGCTCGGTTCCCTCGTGGGGTTCGTGTACATGGTCACCCTCAGAGGCTCCGGCCATCTCCAGCCCAATAGGGCTTACTTGGAGTATCACCTGGACGACTTCTCCGCCTTCCTCCGCCGCAGAGTTCAGAGCTCCTTCAAGTGGGACCTCATCCGAACCTGCTTGAGCTCCTCCACCATGTGTGCTGAGTTGAACCAGAGCTTCCGCATGGCTCAGGATTTTTTCAATGCCCCCATTACCCCTATGCAGTCAGGATGCTGCAAGCCGCCGACGTTGTGCGGGTACACATTTGTGAACCCAACGTACTGGATAATGCCCATAAACAATGCGGCGGACATGGACTGCCTCAAATGGAACAACGACCAAACCCAGCTCTGCTACAGCTGCGACTCCTGCAAGGCCGGCCTACTCCAGAGCTTGAAGAATCAATGGAGGACGGCTGATATAGTGCTCCTCTTGGCCCTCGTCGCCCTCATTTCTGTCTACATAATTGCCTGCTGCGCCTTTAGGAATGCCAAAACTGAGACACTCTTTGACAAATACAAGCAAGGCCAACCCACCTATGTGTAG

Protein sequence

MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGFIGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAFLRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTFVNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVALISVYIIACCAFRNAKTETLFDKYKQGQPTYV
Homology
BLAST of MC06g1673 vs. ExPASy Swiss-Prot
Match: Q9FIQ5 (Protein TORNADO 2 OS=Arabidopsis thaliana OX=3702 GN=TRN2 PE=1 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 7.3e-107
Identity = 183/267 (68.54%), Postives = 225/267 (84.27%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           M L+ N+IG IN + +LLS+P+IGAGIWLA    N+C+++LQWP+II GV +LLVG+AGF
Sbjct: 1   MPLSNNVIGCINFITVLLSIPVIGAGIWLAIGTVNSCVKLLQWPVIILGVLILLVGLAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IG F RITWLL++YL+AML+LI+LLG LVGF+YMVT+RGSGH +P+RAYLEY L DFS +
Sbjct: 61  IGGFWRITWLLVVYLIAMLILIVLLGCLVGFIYMVTIRGSGHPEPSRAYLEYSLQDFSGW 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LRRRVQ S+KW+ IRTCLS++T+C ELNQ + +AQDFFNA + P+QSGCCKPPT CG+TF
Sbjct: 121 LRRRVQRSYKWERIRTCLSTTTICPELNQRYTLAQDFFNAHLDPIQSGCCKPPTKCGFTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWI PI+ +ADMDCL W+NDQ  LCY+CDSCKAGLL ++K  W  ADI LLLAL+ 
Sbjct: 181 VNPTYWISPIDMSADMDCLNWSNDQNTLCYTCDSCKAGLLANIKVDWLKADIFLLLALIG 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQG 268
           LI VYII CCAFRNA+TE +F KYKQG
Sbjct: 241 LIIVYIIGCCAFRNAETEDIFRKYKQG 267

BLAST of MC06g1673 vs. ExPASy Swiss-Prot
Match: Q9ZUN5 (Tetraspanin-2 OS=Arabidopsis thaliana OX=3702 GN=TET2 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.3e-75
Identity = 138/268 (51.49%), Postives = 189/268 (70.52%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MAL  N+   +NL+A+L S+PI  +GIWLA++PDN C+ +L+WP+++ GV +L+V   GF
Sbjct: 1   MALANNLTAILNLLALLCSIPITASGIWLASKPDNECVNLLRWPVVVLGVLILVVSATGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGA+     LL +YL  M +LI LL  ++ F ++VT     +  P R Y EY L+ FS +
Sbjct: 61  IGAYKYKETLLAVYLCCMAILIGLLLVVLIFAFVVTRPDGSYRVPGRGYKEYRLEGFSNW 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFF-NAPITPMQSGCCKPPTLCGYT 180
           L+  V  S  W  +R CL+ + +C +LNQ F  A  FF ++ ITP+QSGCCKPPT CGY 
Sbjct: 121 LKENVVDSKNWGRLRACLADTNVCPKLNQEFITADQFFSSSKITPLQSGCCKPPTACGYN 180

Query: 181 FVNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALV 240
           FVNPT W+ P N AAD DC  W+NDQ+QLCY+C+SCKAGLL +L+ +WR A+++L++ +V
Sbjct: 181 FVNPTLWLNPTNMAADADCYLWSNDQSQLCYNCNSCKAGLLGNLRKEWRKANLILIITVV 240

Query: 241 ALISVYIIACCAFRNAKTETLFDKYKQG 268
            LI VY+IAC AFRNA+TE LF KYKQG
Sbjct: 241 VLIWVYVIACSAFRNAQTEDLFRKYKQG 268

BLAST of MC06g1673 vs. ExPASy Swiss-Prot
Match: Q9M1E7 (Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.0e-56
Identity = 112/262 (42.75%), Postives = 169/262 (64.50%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNA-CLQILQWPLIIFGVAVLLVGVAG 60
           M  + ++IG +N +  LLS+PI+G GIWL+++ ++  CL+ LQWPLI+ G+++++V +AG
Sbjct: 1   MRTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSA 120
           F GA  R  +L+ LYLV ML++I  L   + F Y VT +GSG    NR YL+Y+L+D+S 
Sbjct: 61  FAGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSG 120

Query: 121 FLRRRVQSSFKWDLIRTCLSSSTMCAELNQSF----RMAQDFFNAPITPMQSGCCKPPTL 180
           +L+ RV     W  I +CL  S  C ++ ++F      A  FF   ++P++SGCCKPPT 
Sbjct: 121 WLKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRRLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINN-AADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVL 240
           CG+++VN T W         + DC+ W+NDQ+ LCY C SCKAG+L SLK  WR   ++ 
Sbjct: 181 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

Query: 241 LLALVALISVYIIACCAFRNAK 257
           ++ L+ L+  Y+IA  A+RN K
Sbjct: 241 IVVLIILVIFYVIAYAAYRNVK 262

BLAST of MC06g1673 vs. ExPASy Swiss-Prot
Match: Q8S8Q6 (Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.4e-54
Identity = 106/263 (40.30%), Postives = 163/263 (61.98%), Query Frame = 0

Query: 4   NKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGFIGA 63
           + N++G +N +  LLS+PI+  GIWL+ +    C + L  P+I  GV +++V +AG IG+
Sbjct: 5   SNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAGLIGS 64

Query: 64  FCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAFLRR 123
            CR+TWLL +YL  M +LILL+  +  F ++VT +G+G     + Y EY L D+S +L++
Sbjct: 65  CCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYSTWLQK 124

Query: 124 RVQSSFKWDLIRTCLSSSTMCAELNQSF--RMAQDFFNAPITPMQSGCCKPPTLCGYTFV 183
           RV++   W+ IR+CL  S +C++L   F       F+   +T +QSGCCKP   CG+ +V
Sbjct: 125 RVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECGFEYV 184

Query: 184 NPTYWIMPINNA-ADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 243
           NPT W         + DC  W+N + +LC+ C SCKAGLL ++K+ W+   IV ++ LV 
Sbjct: 185 NPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIVFLVF 244

Query: 244 LISVYIIACCAFRNAKTETLFDK 264
           LI VY + CCAFRN K +  + +
Sbjct: 245 LIIVYSVGCCAFRNNKRDDSYSR 267

BLAST of MC06g1673 vs. ExPASy Swiss-Prot
Match: Q9LSS4 (Tetraspanin-4 OS=Arabidopsis thaliana OX=3702 GN=TET4 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.9e-54
Identity = 112/262 (42.75%), Postives = 164/262 (62.60%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNA-CLQILQWPLIIFGVAVLLVGVAG 60
           M    N+IG IN    LLS+PI+G GIWL+++ ++  CL+ LQWPLII G++++++ +AG
Sbjct: 1   MRSRSNLIGLINFFTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIIIGISIMVISLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSA 120
             GA  +  +L+ LYL  M  +I  L     F Y+VT +GSG    NR YL+Y+L+D+S 
Sbjct: 61  IAGACYQNKFLMWLYLFTMFFVIAALIGFTIFAYVVTDKGSGRFVMNRRYLDYYLNDYSG 120

Query: 121 FLRRRVQSSFKWDLIRTCLSSSTMC----AELNQSFRMAQDFFNAPITPMQSGCCKPPTL 180
           +L+ RV  +  W  I +C+  S +C     +LN     A  F+   ++P++SGCCKPPT 
Sbjct: 121 WLKDRVTDNGYWRDIGSCVRDSGVCKKIGRDLNGVPETAHMFYFRNLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINNAA-DMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVL 240
           CGYT+VN T WI        + DC+ WNNDQ  LCY C SCKAG+L SLK  WR   ++ 
Sbjct: 181 CGYTYVNETVWIPGGEMVGPNPDCMLWNNDQRLLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

Query: 241 LLALVALISVYIIACCAFRNAK 257
           ++ ++ L+  Y+IAC A++N K
Sbjct: 241 IVVVIILVIFYVIACAAYQNVK 262

BLAST of MC06g1673 vs. NCBI nr
Match: XP_022159601.1 (protein TORNADO 2 [Momordica charantia])

HSP 1 Score: 539 bits (1389), Expect = 3.02e-193
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF
Sbjct: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF
Sbjct: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF
Sbjct: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQPTYV 272
           LISVYIIACCAFRNAKTETLFDKYKQGQPTYV
Sbjct: 241 LISVYIIACCAFRNAKTETLFDKYKQGQPTYV 272

BLAST of MC06g1673 vs. NCBI nr
Match: XP_038888578.1 (protein TORNADO 2-like [Benincasa hispida])

HSP 1 Score: 474 bits (1220), Expect = 1.79e-167
Identity = 227/269 (84.39%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNK ++G IN +AM++S+PIIGAGIWLATQ DNAC+QILQWP+IIFGV VLLV +AGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPIIIFGVVVLLVAIAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAFCRITWLLL+YLVAML+LI+LLG LVGF+YMVT+RGSGHL+PNR+YLEYHLDDFS F
Sbjct: 61  IGAFCRITWLLLVYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLDDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RVQSSFKWDLIR+CLSSS+MCAELNQSFRMAQDFF APITP+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRMAQDFFTAPITPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINN ADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADI+LLL+L+A
Sbjct: 181 VNPTYWIMPINNGADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQP 269
           LIS+Y+IA C FRNAKTE LFDKYKQ QP
Sbjct: 241 LISIYMIAFCVFRNAKTEKLFDKYKQAQP 269

BLAST of MC06g1673 vs. NCBI nr
Match: XP_004136394.1 (protein TORNADO 2 [Cucumis sativus] >KGN60375.1 hypothetical protein Csa_001399 [Cucumis sativus])

HSP 1 Score: 471 bits (1212), Expect = 3.08e-166
Identity = 226/269 (84.01%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNK ++G IN +AM++S+PIIGAGIWLATQ DNAC+QILQWPLIIFGV VLLV VAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVIVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAFCRI WLL+ YLVAML+LI+LLG LVGF+YMVT+RGSGHL+PNR+YLEYHL+DFS F
Sbjct: 61  IGAFCRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RVQSSFKWDLIR+CLSSS+MCAELNQSFR+AQDFF API+P+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADI+LLL+L+A
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQP 269
           LISVY+IA C FRNAKT+ LFDKYKQGQP
Sbjct: 241 LISVYMIAACVFRNAKTQKLFDKYKQGQP 269

BLAST of MC06g1673 vs. NCBI nr
Match: XP_022996183.1 (protein TORNADO 2 [Cucurbita maxima])

HSP 1 Score: 469 bits (1208), Expect = 1.25e-165
Identity = 228/268 (85.07%), Postives = 251/268 (93.66%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MAL K +IG IN +AMLLSVPIIGAGIWLATQ DNAC+QILQWP+I FGV VL+V VAGF
Sbjct: 1   MALTKTVIGAINFIAMLLSVPIIGAGIWLATQQDNACVQILQWPIIFFGVIVLIVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAF RI+WLL++YL+AMLVLI+LL  LVGF+YMVT+RGSGHL+PNRAYLEYHLDDFS F
Sbjct: 61  IGAFWRISWLLIVYLIAMLVLIVLLACLVGFIYMVTIRGSGHLEPNRAYLEYHLDDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RV+SSFKWDLIR+CLSSS+MC +LNQSFRMAQDFFNAPI+P+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVESSFKWDLIRSCLSSSSMCGQLNQSFRMAQDFFNAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADIVLLLALVA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIVLLLALVA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQ 268
           LISVY+IACCAF+NAKTE LFDKYKQG+
Sbjct: 241 LISVYLIACCAFKNAKTEKLFDKYKQGR 268

BLAST of MC06g1673 vs. NCBI nr
Match: XP_008465983.1 (PREDICTED: protein TORNADO 2 [Cucumis melo] >KAA0038570.1 protein TORNADO 2 [Cucumis melo var. makuwa] >TYK31173.1 protein TORNADO 2 [Cucumis melo var. makuwa])

HSP 1 Score: 468 bits (1203), Expect = 7.24e-165
Identity = 224/269 (83.27%), Postives = 251/269 (93.31%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNK ++G IN +AM++S+PIIGAGIWLATQ DNAC+QILQWPLIIFGV VLLV VAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVVVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAF RI WLL+ YLVAML+LI+LLG LVGF+YMVT+RGSGHL+PNR+YLEYHL+DFS F
Sbjct: 61  IGAFLRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RVQSSFKWDLIR+CLSSS+MCAELNQSFR+AQDFF API+P+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADI+LLL+L+A
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQP 269
           L+SVY+IA C FRNAKT+ LFDKYKQGQP
Sbjct: 241 LVSVYLIAACVFRNAKTQKLFDKYKQGQP 269

BLAST of MC06g1673 vs. ExPASy TrEMBL
Match: A0A6J1E4F1 (protein TORNADO 2 OS=Momordica charantia OX=3673 GN=LOC111025966 PE=4 SV=1)

HSP 1 Score: 539 bits (1389), Expect = 1.46e-193
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF
Sbjct: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF
Sbjct: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF
Sbjct: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQPTYV 272
           LISVYIIACCAFRNAKTETLFDKYKQGQPTYV
Sbjct: 241 LISVYIIACCAFRNAKTETLFDKYKQGQPTYV 272

BLAST of MC06g1673 vs. ExPASy TrEMBL
Match: A0A0A0LEA8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G901690 PE=4 SV=1)

HSP 1 Score: 471 bits (1212), Expect = 1.49e-166
Identity = 226/269 (84.01%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNK ++G IN +AM++S+PIIGAGIWLATQ DNAC+QILQWPLIIFGV VLLV VAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVIVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAFCRI WLL+ YLVAML+LI+LLG LVGF+YMVT+RGSGHL+PNR+YLEYHL+DFS F
Sbjct: 61  IGAFCRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RVQSSFKWDLIR+CLSSS+MCAELNQSFR+AQDFF API+P+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADI+LLL+L+A
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQP 269
           LISVY+IA C FRNAKT+ LFDKYKQGQP
Sbjct: 241 LISVYMIAACVFRNAKTQKLFDKYKQGQP 269

BLAST of MC06g1673 vs. ExPASy TrEMBL
Match: A0A6J1KA46 (protein TORNADO 2 OS=Cucurbita maxima OX=3661 GN=LOC111491491 PE=4 SV=1)

HSP 1 Score: 469 bits (1208), Expect = 6.07e-166
Identity = 228/268 (85.07%), Postives = 251/268 (93.66%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MAL K +IG IN +AMLLSVPIIGAGIWLATQ DNAC+QILQWP+I FGV VL+V VAGF
Sbjct: 1   MALTKTVIGAINFIAMLLSVPIIGAGIWLATQQDNACVQILQWPIIFFGVIVLIVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAF RI+WLL++YL+AMLVLI+LL  LVGF+YMVT+RGSGHL+PNRAYLEYHLDDFS F
Sbjct: 61  IGAFWRISWLLIVYLIAMLVLIVLLACLVGFIYMVTIRGSGHLEPNRAYLEYHLDDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RV+SSFKWDLIR+CLSSS+MC +LNQSFRMAQDFFNAPI+P+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVESSFKWDLIRSCLSSSSMCGQLNQSFRMAQDFFNAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADIVLLLALVA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIVLLLALVA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQ 268
           LISVY+IACCAF+NAKTE LFDKYKQG+
Sbjct: 241 LISVYLIACCAFKNAKTEKLFDKYKQGR 268

BLAST of MC06g1673 vs. ExPASy TrEMBL
Match: A0A5A7T5J8 (Protein TORNADO 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004550 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 3.51e-165
Identity = 224/269 (83.27%), Postives = 251/269 (93.31%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNK ++G IN +AM++S+PIIGAGIWLATQ DNAC+QILQWPLIIFGV VLLV VAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVVVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAF RI WLL+ YLVAML+LI+LLG LVGF+YMVT+RGSGHL+PNR+YLEYHL+DFS F
Sbjct: 61  IGAFLRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RVQSSFKWDLIR+CLSSS+MCAELNQSFR+AQDFF API+P+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADI+LLL+L+A
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQP 269
           L+SVY+IA C FRNAKT+ LFDKYKQGQP
Sbjct: 241 LVSVYLIAACVFRNAKTQKLFDKYKQGQP 269

BLAST of MC06g1673 vs. ExPASy TrEMBL
Match: A0A1S3CQH2 (protein TORNADO 2 OS=Cucumis melo OX=3656 GN=LOC103503551 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 3.51e-165
Identity = 224/269 (83.27%), Postives = 251/269 (93.31%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MALNK ++G IN +AM++S+PIIGAGIWLATQ DNAC+QILQWPLIIFGV VLLV VAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVVVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGAF RI WLL+ YLVAML+LI+LLG LVGF+YMVT+RGSGHL+PNR+YLEYHL+DFS F
Sbjct: 61  IGAFLRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LR RVQSSFKWDLIR+CLSSS+MCAELNQSFR+AQDFF API+P+QSGCCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCY CDSCKAGLL+SLKNQWR ADI+LLL+L+A
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQGQP 269
           L+SVY+IA C FRNAKT+ LFDKYKQGQP
Sbjct: 241 LVSVYLIAACVFRNAKTQKLFDKYKQGQP 269

BLAST of MC06g1673 vs. TAIR 10
Match: AT5G46700.1 (Tetraspanin family protein )

HSP 1 Score: 388.3 bits (996), Expect = 5.2e-108
Identity = 183/267 (68.54%), Postives = 225/267 (84.27%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           M L+ N+IG IN + +LLS+P+IGAGIWLA    N+C+++LQWP+II GV +LLVG+AGF
Sbjct: 1   MPLSNNVIGCINFITVLLSIPVIGAGIWLAIGTVNSCVKLLQWPVIILGVLILLVGLAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IG F RITWLL++YL+AML+LI+LLG LVGF+YMVT+RGSGH +P+RAYLEY L DFS +
Sbjct: 61  IGGFWRITWLLVVYLIAMLILIVLLGCLVGFIYMVTIRGSGHPEPSRAYLEYSLQDFSGW 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFFNAPITPMQSGCCKPPTLCGYTF 180
           LRRRVQ S+KW+ IRTCLS++T+C ELNQ + +AQDFFNA + P+QSGCCKPPT CG+TF
Sbjct: 121 LRRRVQRSYKWERIRTCLSTTTICPELNQRYTLAQDFFNAHLDPIQSGCCKPPTKCGFTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 240
           VNPTYWI PI+ +ADMDCL W+NDQ  LCY+CDSCKAGLL ++K  W  ADI LLLAL+ 
Sbjct: 181 VNPTYWISPIDMSADMDCLNWSNDQNTLCYTCDSCKAGLLANIKVDWLKADIFLLLALIG 240

Query: 241 LISVYIIACCAFRNAKTETLFDKYKQG 268
           LI VYII CCAFRNA+TE +F KYKQG
Sbjct: 241 LIIVYIIGCCAFRNAETEDIFRKYKQG 267

BLAST of MC06g1673 vs. TAIR 10
Match: AT2G19580.1 (tetraspanin2 )

HSP 1 Score: 283.1 bits (723), Expect = 2.4e-76
Identity = 138/268 (51.49%), Postives = 189/268 (70.52%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGF 60
           MAL  N+   +NL+A+L S+PI  +GIWLA++PDN C+ +L+WP+++ GV +L+V   GF
Sbjct: 1   MALANNLTAILNLLALLCSIPITASGIWLASKPDNECVNLLRWPVVVLGVLILVVSATGF 60

Query: 61  IGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAF 120
           IGA+     LL +YL  M +LI LL  ++ F ++VT     +  P R Y EY L+ FS +
Sbjct: 61  IGAYKYKETLLAVYLCCMAILIGLLLVVLIFAFVVTRPDGSYRVPGRGYKEYRLEGFSNW 120

Query: 121 LRRRVQSSFKWDLIRTCLSSSTMCAELNQSFRMAQDFF-NAPITPMQSGCCKPPTLCGYT 180
           L+  V  S  W  +R CL+ + +C +LNQ F  A  FF ++ ITP+QSGCCKPPT CGY 
Sbjct: 121 LKENVVDSKNWGRLRACLADTNVCPKLNQEFITADQFFSSSKITPLQSGCCKPPTACGYN 180

Query: 181 FVNPTYWIMPINNAADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALV 240
           FVNPT W+ P N AAD DC  W+NDQ+QLCY+C+SCKAGLL +L+ +WR A+++L++ +V
Sbjct: 181 FVNPTLWLNPTNMAADADCYLWSNDQSQLCYNCNSCKAGLLGNLRKEWRKANLILIITVV 240

Query: 241 ALISVYIIACCAFRNAKTETLFDKYKQG 268
            LI VY+IAC AFRNA+TE LF KYKQG
Sbjct: 241 VLIWVYVIACSAFRNAQTEDLFRKYKQG 268

BLAST of MC06g1673 vs. TAIR 10
Match: AT3G45600.1 (tetraspanin3 )

HSP 1 Score: 220.7 bits (561), Expect = 1.4e-57
Identity = 112/262 (42.75%), Postives = 169/262 (64.50%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNA-CLQILQWPLIIFGVAVLLVGVAG 60
           M  + ++IG +N +  LLS+PI+G GIWL+++ ++  CL+ LQWPLI+ G+++++V +AG
Sbjct: 1   MRTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSA 120
           F GA  R  +L+ LYLV ML++I  L   + F Y VT +GSG    NR YL+Y+L+D+S 
Sbjct: 61  FAGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSG 120

Query: 121 FLRRRVQSSFKWDLIRTCLSSSTMCAELNQSF----RMAQDFFNAPITPMQSGCCKPPTL 180
           +L+ RV     W  I +CL  S  C ++ ++F      A  FF   ++P++SGCCKPPT 
Sbjct: 121 WLKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRRLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINN-AADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVL 240
           CG+++VN T W         + DC+ W+NDQ+ LCY C SCKAG+L SLK  WR   ++ 
Sbjct: 181 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

Query: 241 LLALVALISVYIIACCAFRNAK 257
           ++ L+ L+  Y+IA  A+RN K
Sbjct: 241 IVVLIILVIFYVIAYAAYRNVK 262

BLAST of MC06g1673 vs. TAIR 10
Match: AT2G23810.1 (tetraspanin8 )

HSP 1 Score: 214.5 bits (545), Expect = 1.0e-55
Identity = 106/263 (40.30%), Postives = 163/263 (61.98%), Query Frame = 0

Query: 4   NKNIIGGINLVAMLLSVPIIGAGIWLATQPDNACLQILQWPLIIFGVAVLLVGVAGFIGA 63
           + N++G +N +  LLS+PI+  GIWL+ +    C + L  P+I  GV +++V +AG IG+
Sbjct: 5   SNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAGLIGS 64

Query: 64  FCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSAFLRR 123
            CR+TWLL +YL  M +LILL+  +  F ++VT +G+G     + Y EY L D+S +L++
Sbjct: 65  CCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYSTWLQK 124

Query: 124 RVQSSFKWDLIRTCLSSSTMCAELNQSF--RMAQDFFNAPITPMQSGCCKPPTLCGYTFV 183
           RV++   W+ IR+CL  S +C++L   F       F+   +T +QSGCCKP   CG+ +V
Sbjct: 125 RVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECGFEYV 184

Query: 184 NPTYWIMPINNA-ADMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVLLLALVA 243
           NPT W         + DC  W+N + +LC+ C SCKAGLL ++K+ W+   IV ++ LV 
Sbjct: 185 NPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIVFLVF 244

Query: 244 LISVYIIACCAFRNAKTETLFDK 264
           LI VY + CCAFRN K +  + +
Sbjct: 245 LIIVYSVGCCAFRNNKRDDSYSR 267

BLAST of MC06g1673 vs. TAIR 10
Match: AT5G60220.1 (tetraspanin4 )

HSP 1 Score: 214.2 bits (544), Expect = 1.3e-55
Identity = 112/262 (42.75%), Postives = 164/262 (62.60%), Query Frame = 0

Query: 1   MALNKNIIGGINLVAMLLSVPIIGAGIWLATQPDNA-CLQILQWPLIIFGVAVLLVGVAG 60
           M    N+IG IN    LLS+PI+G GIWL+++ ++  CL+ LQWPLII G++++++ +AG
Sbjct: 1   MRSRSNLIGLINFFTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIIIGISIMVISLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLVLILLLGSLVGFVYMVTLRGSGHLQPNRAYLEYHLDDFSA 120
             GA  +  +L+ LYL  M  +I  L     F Y+VT +GSG    NR YL+Y+L+D+S 
Sbjct: 61  IAGACYQNKFLMWLYLFTMFFVIAALIGFTIFAYVVTDKGSGRFVMNRRYLDYYLNDYSG 120

Query: 121 FLRRRVQSSFKWDLIRTCLSSSTMC----AELNQSFRMAQDFFNAPITPMQSGCCKPPTL 180
           +L+ RV  +  W  I +C+  S +C     +LN     A  F+   ++P++SGCCKPPT 
Sbjct: 121 WLKDRVTDNGYWRDIGSCVRDSGVCKKIGRDLNGVPETAHMFYFRNLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINNAA-DMDCLKWNNDQTQLCYSCDSCKAGLLQSLKNQWRTADIVL 240
           CGYT+VN T WI        + DC+ WNNDQ  LCY C SCKAG+L SLK  WR   ++ 
Sbjct: 181 CGYTYVNETVWIPGGEMVGPNPDCMLWNNDQRLLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

Query: 241 LLALVALISVYIIACCAFRNAK 257
           ++ ++ L+  Y+IAC A++N K
Sbjct: 241 IVVVIILVIFYVIACAAYQNVK 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIQ57.3e-10768.54Protein TORNADO 2 OS=Arabidopsis thaliana OX=3702 GN=TRN2 PE=1 SV=1[more]
Q9ZUN53.3e-7551.49Tetraspanin-2 OS=Arabidopsis thaliana OX=3702 GN=TET2 PE=2 SV=1[more]
Q9M1E72.0e-5642.75Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1[more]
Q8S8Q61.4e-5440.30Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1[more]
Q9LSS41.9e-5442.75Tetraspanin-4 OS=Arabidopsis thaliana OX=3702 GN=TET4 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022159601.13.02e-193100.00protein TORNADO 2 [Momordica charantia][more]
XP_038888578.11.79e-16784.39protein TORNADO 2-like [Benincasa hispida][more]
XP_004136394.13.08e-16684.01protein TORNADO 2 [Cucumis sativus] >KGN60375.1 hypothetical protein Csa_001399 ... [more]
XP_022996183.11.25e-16585.07protein TORNADO 2 [Cucurbita maxima][more]
XP_008465983.17.24e-16583.27PREDICTED: protein TORNADO 2 [Cucumis melo] >KAA0038570.1 protein TORNADO 2 [Cuc... [more]
Match NameE-valueIdentityDescription
A0A6J1E4F11.46e-193100.00protein TORNADO 2 OS=Momordica charantia OX=3673 GN=LOC111025966 PE=4 SV=1[more]
A0A0A0LEA81.49e-16684.01Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G901690 PE=4 SV=1[more]
A0A6J1KA466.07e-16685.07protein TORNADO 2 OS=Cucurbita maxima OX=3661 GN=LOC111491491 PE=4 SV=1[more]
A0A5A7T5J83.51e-16583.27Protein TORNADO 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00... [more]
A0A1S3CQH23.51e-16583.27protein TORNADO 2 OS=Cucumis melo OX=3656 GN=LOC103503551 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G46700.15.2e-10868.54Tetraspanin family protein [more]
AT2G19580.12.4e-7651.49tetraspanin2 [more]
AT3G45600.11.4e-5742.75tetraspanin3 [more]
AT2G23810.11.0e-5540.30tetraspanin8 [more]
AT5G60220.11.3e-5542.75tetraspanin4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 5..250
e-value: 1.7E-24
score: 86.8
IPR044991Tetraspani, plantPANTHERPTHR32191TETRASPANIN-8-RELATEDcoord: 1..267
NoneNo IPR availablePANTHERPTHR32191:SF14PROTEIN TORNADO 2coord: 1..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1673.1MC06g1673.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma