MC06g1566 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1566
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionSeryl-tRNA synthetase
LocationMC06: 22905071 .. 22910333 (-)
RNA-Seq ExpressionMC06g1566
SyntenyMC06g1566
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTCTCCCTCGTAGCACATCGCCGCCGCCGCCGCCGCATCACTCATCTCTTCGGTACGGTCTTTGTACCCATTTTTGTCTCTCAAATTTCATTGATGAACGTCTCGTCTGCAACCCTGAACCGCTGGTTGGAAGCCGGTTTGGTCCAATCATGGACATCGGTTCGGTTGTGAACCGGGCTGATGCTCAGGATTAGGGTTTTACAGCTTAAAGCTCTTTCTCTCTGAATCTTGCTTGCATTTTCTGGACCCTGTTGTTTGTTGTTCTTCAGAGGCAGCGGAAATCGAGAGAGATGTTGGATATCAATCTCTTCAGAGAAGAGAAGGGTAACAATCCTGAAATCATCCGCGAATCTCAGCGCCGCCGATTTGCTAACGTTGAACTCGTTGATGAAGTCATTAAGCTTGATAAGGAATGGCGTCAACGTAATTCTCATTTTTTACCACTTTTTCATTGCTTTTTTTCTTTTCTTCTTTTTCCTGCTCTATCTATCTGTTTTGAAATTGATAAATTAGGATTCGTGTAGGCCAATTTGAGCTCGAGAATCTCAGGAAGGAGTTCAACAAGATCAACAAGCAAATTGCTCAGCTCAGAATAGTAACTGATCTGCCTTTTTTCTCATTTGTCTTTGTTACCTTTTTGCTTGTTTTTTCTGTCTTACATTATCAGATTCATCTCTTCGTTAATATCTTGCCTGTATTGGTTGCAAGAAAATTAGTTTTGGAACTGAATCGGCTCTCTTAAGTGTTAATTTCAAAGTCCATGCAACTGTTAGTCATCGATTTAGGGATTTAAGTTTCTCTCTTGTGAAATTTATGGTTTGGTTAAATTTCAGTATGCCCTGTTGTAAACTTTGATTTTTTTTAAATTTTTTTTATTTTTTGAAATGAAGGCTGGTGAGGATGCATCGGAGAAGATTAAAGATACTGAAGAAAATAAAAAGTTGTCTGCAGAGAAAGATGTTGAAGCTAAAGATGTGCTGAAACAGTTGAACTCAAAATTGGAAATAATCGGAAACCTTGTGCACGATTCAGTTCCAGTCAGCAATGATGAGGTATAGTGTTTGACTTCACTGCAATTTTTCTGTTCTTGGTAGCTCTTAATTTTTATTTTTATTTTAATATTGGACTTGGCAACATTTTGTAGGCTAACAATGCTATAATCAGATCGTGGGGTGAGAAACTGGTAAAACCCAAACTGAAGAATCATGTTGATCTTGTTGAGCTTCTTGGAATTGCAGATACCAAAAAAGGTAACAAATTTTAACTGTCTTATTCCAGTCGATCTTCTATGTTATGTAAAAAATTCATCTCTCATACCTTCAATTATTTCTACTATGGAACTCTGTTTCAATCTCTTGGTTTTGCTTCAAATCTATGTACCAATGGAAATCTCTTTGTTTTCGTCCATCTGACAAAATGAGGAGTAATAAATCTAGTGTTATATGCTATCCTAGTGATATTGGAGCATGTGAAAAATGTAAAATGACTTCATGAGGATGGATTAAATTCTCATGATGGCCACCTACTTAAGATATTAAATTCGTATGAGATTCTTAATAGTCAAATGTTAAAGAGTTGAGCAGTTGTCTAGTGAGATGCATGTAAGCTAGCTTTGAATGCTCACGAATATAATAATAACAATAATTGTGGTTTGTAGAGGCATATGCTATATGCATCATAGTATTTCAGAGGTTAATAGCTGAGCCCTCTAGTTTGAGAAGCATTAGATTTAGGATTTTGGTTGAGCTGGTAGTGCTTTTCCTTAAAATTTATGCATAGCACTCGCATTCCTTTATCTCCACACCTTGTACCTTGTGAAATCTTTGTTTCCAATCTATACCTTCTATATCTCCCCATCATAATTTAAATTCAGAATAATTAAGCGTTATGCTAATTCCAACTTACTGGAACTATGGAAATATATAGGTTCTGAAATAGCAGGAGGCAGAGGCTTCTACCTAAAAGGAGACGGTGTACGTTTGAACCAAGCTTTGATAAACTATGGCCTTGACTTTTTGGAGAAAAGGGGTTATACAGCTCTGCAGACTCCTTTCTTCATGAGAAAAGATATTATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTCTACAAGGTTTGCTTGTGCCCTCTGTTTGTTTCCATAGGATTTGAAGGGATTTTAAAATCTAATGGATTTGAAATTAATTCTTTTATTTTTATCTCTTGAAGTATAAAGATTATTTAAGATAAATGGTCAGATTTGATAGGATTTCTAAGTCAATTACCAATTTAACCTGATCTGATTTCATTTTTAATTCATGAATTAGGATGGAAAGTTCTTATCCAAAAAAGAATTAGGATGGAAAGCATTAGTAAACTCCTTGTTTTTCATTTCCAGCAAACATCCAAACATGTTTTTTAAATCAAATTCATTTCAAATCCTTACCATTCCAAACAATACAACAAATTTATGCCAAACAAAATTTTAATGATTTTCTTTCTTTGGAAAGTATAGTTTTTACTTGTATTTCAGGACAAGGGGTCTCAGTTCATTAGTAAAACAGTTGTTTTTCTGTGTTTGGGCTGTGGAAGCATGCCAAAATATGACCATAAAATAAATTGGTATTCCTTAGGTTACTGGTGAGGGGGATGATAAATATCTAATTGCCACTGCTGAACAACCACTTTGTGCATATCATCTTGAAGATTGGATTCATCCTTCCCAATTGCCTATAAGGTTCGTGCATCTCACTGAAGACTGTCTACCGTTCTAGCACCAAAATAAAGCAGTTCTGGTTTTGTTACTATTCCTAATGTTCTCTCCTCAGATATGCTGGATATTCGTCTTGCTTTCGCAAAGAAGCGGGTTCCCATGGCCGGGATACTTTGGGAATTTTTCGAGTCCACCAGTTTGAGAAAGTAGAGCAATTTTGTATGACCAGCCCGAATGGCAATGATTCATGGGACATGCATGAGGAGATGATTAAAAACTCCGAGGACTTCTATAAGACGGTAGCTTCTATTCTTTGCCTCAATGGTTTCTTTTTTACGCATTTATGATTTCCATGTTGTCAAGCTGGTTGTTTTGCCGTTTGATGGGATATTTCGCTTGCGAGGAATAATATTTTTTTTTTTTAAAAAAAGGGTTGAGATGTCTTGGGGGAGAGGGCTGGTCTCTTGCCAAATTTCTGCTTCTCATTCGGTGTTGGTGTCTAAGTTGTGTTTTTTTTTTTTTGGTTTAAAAAGGAAATTATATGTTAGGCCTTATTCTTTGGATTGGAGCCCAATTTTGTATAGTTAGTTTAGGTTCTGGGTTGGCTCATCTTGTCGGTAACTTGAATCGACCTTTTTTTTTCTTTTGCCTTTTTGCGTTCTCGTACTCTTTCACAGATTTTTTTTTTCTCGGCAGAAGCTTAGTTTTCCATTAAAAAAAATTGTTTTACTCTTTTTCTGATGTGAGATCATATGTTTTGACAGTTAAATCTCCCTTACCAAGTTGTGGCCATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATATGATTTGGAAGCATGGTTTCCTGCATCTCAGACTTACAGGGAGCTAGTATCCTGTTCAAATTGCACTGACTATCAATCCAGAAGGTTAGAGATCCGATATGGTCAGAAAAAGGTCAGTTCTTTTTCATCATTTCTGAATTATTATAGAATTTCTTTGGCAATTAAGATTGTTGGACCTGGTGCTAACCTTTTAATAGGGAGGGATTGATTCTTAAACTTAATTGTTGTCTATGATTCTGGCATTCCTGTAACTTCATGGTTTATTTTTGGATTAAAGTTGATTTGGTCATTTTAAATTTTGTGTCTAATAGCTCCTCATTGGTTTAACGTTTACACCACATACAGTCAGTATACCACTTGTAATTGACACATGAACTTATCAATTGAGAGATCAATAAAAAAAACCTTTTGAACTTTTTGAAGTATATGGATCTCATACAATCTTGAAAGTTGAGGGATCTAGACTAAATAGTCGCATCCCTAGACTTTCGGATCTAACCTATCATTTAACCATTTATTTTTCCTTCTTTGTTTTTTAGTATGTTCAATTGTTTTCAATCTTGGAAGTTGAGCGATCCATCATAAACTTTTCAAAATATAGAGATTGAACTGACTTCAAATTTTCTTTCTTTTTTTTTTTTCATATGTTTAACTGCTCTTTTATTCTCCATGGTGCAACCGCAGAGCAATGAGCAAACGAAGCAATATGTGCATTTGCTAAATTCTACTCTCACAGCAACAGAAAGGACTCTGTGCTGCATTCTTGAGAACTACCAGAAAGAAGATGGGGTTGAGATACCAGAAGTTCTACAACCATTTATGGGTGGAAAGACCTTTCTACCCTTCAAAGCGAAGCCATCGACGAGCGAAGCCAAAGGGAAGAAATCGAAGGCCTAATTGCATTTTTCTTTTCTCCACTTTGATTTATAAATTATCTGACTCAAGGGAAGGAAATTGCTCACAATATTGTTGGAACTTCAAATTATCTTTTTGGTGGGTATCAAATTATCTTAATTGCTAATACACACGGCTGTTTCTGTTGATGCATCTTTTGGAAACCTACTTTTTTTGTTTGGTCATTTGTGTACTCTATAAAACAGTTATAAATTGAATTTTCTGATCTGGGTCTGATCTTTTTATTATGACCCAATAAGTATTTTGAAGCTTTCTTCAAAATATTACTCGAATGAATTAGGAATATCCTTGGATTCTAAACTAATTTTCTTGGGCTTTTGCAGGGCTCGCAATCGTTTCCAATCTTGTGAAGCAAGCCCAAAGCTCACTCCAATAATGAAATTCTTGGACCACTCCCACATGGTCTGGAAACTCTTATTTAGAAAATTCAGATTAGGTTTGAGATGACGTATCGATATCGAATGCAGCTGCTACAATATAACTATCACTTTTATAATCGTTGAGTCGTGGTCATGTTTTGGCAGCTGGGTTCATAGTCGTTAGATGTGTGGAAAGTATGATATTGCTATCGCTTTTTCAATAGTTAGATCTGTGGAAAAGAGAATTCAAAACTGTGGTCACGTTTTGGCAGCCAGTTTTGGCAACGTTGTCGTTTTGATGAGAAATTGAAGTCAGATTGAAAAAAAGAGATTAATTGCTGCAAAACAATTTCTTCTATTTTGTTTACCATTTTGGAGTTTTTGTTCTTTGACAAATCCTACTGAGATTATTCAGACCTACTTCGGAATCTAATCAAATGAAAATTTTGATACGAG

mRNA sequence

CCCTCTCCCTCGTAGCACATCGCCGCCGCCGCCGCCGCATCACTCATCTCTTCGAGGCAGCGGAAATCGAGAGAGATGTTGGATATCAATCTCTTCAGAGAAGAGAAGGGTAACAATCCTGAAATCATCCGCGAATCTCAGCGCCGCCGATTTGCTAACGTTGAACTCGTTGATGAAGTCATTAAGCTTGATAAGGAATGGCGTCAACGCCAATTTGAGCTCGAGAATCTCAGGAAGGAGTTCAACAAGATCAACAAGCAAATTGCTCAGCTCAGAATAGCTGGTGAGGATGCATCGGAGAAGATTAAAGATACTGAAGAAAATAAAAAGTTGTCTGCAGAGAAAGATGTTGAAGCTAAAGATGTGCTGAAACAGTTGAACTCAAAATTGGAAATAATCGGAAACCTTGTGCACGATTCAGTTCCAGTCAGCAATGATGAGGCTAACAATGCTATAATCAGATCGTGGGGTGAGAAACTGGTAAAACCCAAACTGAAGAATCATGTTGATCTTGTTGAGCTTCTTGGAATTGCAGATACCAAAAAAGGTTCTGAAATAGCAGGAGGCAGAGGCTTCTACCTAAAAGGAGACGGTGTACGTTTGAACCAAGCTTTGATAAACTATGGCCTTGACTTTTTGGAGAAAAGGGGTTATACAGCTCTGCAGACTCCTTTCTTCATGAGAAAAGATATTATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTCTACAAGGTTACTGGTGAGGGGGATGATAAATATCTAATTGCCACTGCTGAACAACCACTTTGTGCATATCATCTTGAAGATTGGATTCATCCTTCCCAATTGCCTATAAGATATGCTGGATATTCGTCTTGCTTTCGCAAAGAAGCGGGTTCCCATGGCCGGGATACTTTGGGAATTTTTCGAGTCCACCAGTTTGAGAAAGTAGAGCAATTTTGTATGACCAGCCCGAATGGCAATGATTCATGGGACATGCATGAGGAGATGATTAAAAACTCCGAGGACTTCTATAAGACGTTAAATCTCCCTTACCAAGTTGTGGCCATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATATGATTTGGAAGCATGGTTTCCTGCATCTCAGACTTACAGGGAGCTAGTATCCTGTTCAAATTGCACTGACTATCAATCCAGAAGGTTAGAGATCCGATATGGTCAGAAAAAGAGCAATGAGCAAACGAAGCAATATGTGCATTTGCTAAATTCTACTCTCACAGCAACAGAAAGGACTCTGTGCTGCATTCTTGAGAACTACCAGAAAGAAGATGGGGTTGAGATACCAGAAGTTCTACAACCATTTATGGGTGGAAAGACCTTTCTACCCTTCAAAGCGAAGCCATCGACGAGCGAAGCCAAAGGGAAGAAATCGAAGGCCTAATTGCATTTTTCTTTTCTCCACTTTGATTTATAAATTATCTGACTCAAGGGAAGGAAATTGCTCACAATATTGTTGGAACTTCAAATTATCTTTTTGGTGGGTATCAAATTATCTTAATTGCTAATACACACGGCTGTTTCTGTTGATGCATCTTTTGGAAACCTACTTTTTTTGTTTGGTCATTTGTGTACTCTATAAAACAGTTATAAATTGAATTTTCTGATCTGGGTCTGATCTTTTTATTATGACCCAATAAGTATTTTGAAGCTTTCTTCAAAATATTACTCGAATGAATTAGGAATATCCTTGGATTCTAAACTAATTTTCTTGGGCTTTTGCAGGGCTCGCAATCGTTTCCAATCTTGTGAAGCAAGCCCAAAGCTCACTCCAATAATGAAATTCTTGGACCACTCCCACATGGTCTGGAAACTCTTATTTAGAAAATTCAGATTAGGTTTGAGATGACGTATCGATATCGAATGCAGCTGCTACAATATAACTATCACTTTTATAATCGTTGAGTCGTGGTCATGTTTTGGCAGCTGGGTTCATAGTCGTTAGATGTGTGGAAAGTATGATATTGCTATCGCTTTTTCAATAGTTAGATCTGTGGAAAAGAGAATTCAAAACTGTGGTCACGTTTTGGCAGCCAGTTTTGGCAACGTTGTCGTTTTGATGAGAAATTGAAGTCAGATTGAAAAAAAGAGATTAATTGCTGCAAAACAATTTCTTCTATTTTGTTTACCATTTTGGAGTTTTTGTTCTTTGACAAATCCTACTGAGATTATTCAGACCTACTTCGGAATCTAATCAAATGAAAATTTTGATACGAG

Coding sequence (CDS)

ATGTTGGATATCAATCTCTTCAGAGAAGAGAAGGGTAACAATCCTGAAATCATCCGCGAATCTCAGCGCCGCCGATTTGCTAACGTTGAACTCGTTGATGAAGTCATTAAGCTTGATAAGGAATGGCGTCAACGCCAATTTGAGCTCGAGAATCTCAGGAAGGAGTTCAACAAGATCAACAAGCAAATTGCTCAGCTCAGAATAGCTGGTGAGGATGCATCGGAGAAGATTAAAGATACTGAAGAAAATAAAAAGTTGTCTGCAGAGAAAGATGTTGAAGCTAAAGATGTGCTGAAACAGTTGAACTCAAAATTGGAAATAATCGGAAACCTTGTGCACGATTCAGTTCCAGTCAGCAATGATGAGGCTAACAATGCTATAATCAGATCGTGGGGTGAGAAACTGGTAAAACCCAAACTGAAGAATCATGTTGATCTTGTTGAGCTTCTTGGAATTGCAGATACCAAAAAAGGTTCTGAAATAGCAGGAGGCAGAGGCTTCTACCTAAAAGGAGACGGTGTACGTTTGAACCAAGCTTTGATAAACTATGGCCTTGACTTTTTGGAGAAAAGGGGTTATACAGCTCTGCAGACTCCTTTCTTCATGAGAAAAGATATTATGGCTAGATGTGCTCAACTAGCACAATTTGATGAAGAACTCTACAAGGTTACTGGTGAGGGGGATGATAAATATCTAATTGCCACTGCTGAACAACCACTTTGTGCATATCATCTTGAAGATTGGATTCATCCTTCCCAATTGCCTATAAGATATGCTGGATATTCGTCTTGCTTTCGCAAAGAAGCGGGTTCCCATGGCCGGGATACTTTGGGAATTTTTCGAGTCCACCAGTTTGAGAAAGTAGAGCAATTTTGTATGACCAGCCCGAATGGCAATGATTCATGGGACATGCATGAGGAGATGATTAAAAACTCCGAGGACTTCTATAAGACGTTAAATCTCCCTTACCAAGTTGTGGCCATAGTTTCTGGTGCATTAAATGATGCAGCTGCCAAGAAATATGATTTGGAAGCATGGTTTCCTGCATCTCAGACTTACAGGGAGCTAGTATCCTGTTCAAATTGCACTGACTATCAATCCAGAAGGTTAGAGATCCGATATGGTCAGAAAAAGAGCAATGAGCAAACGAAGCAATATGTGCATTTGCTAAATTCTACTCTCACAGCAACAGAAAGGACTCTGTGCTGCATTCTTGAGAACTACCAGAAAGAAGATGGGGTTGAGATACCAGAAGTTCTACAACCATTTATGGGTGGAAAGACCTTTCTACCCTTCAAAGCGAAGCCATCGACGAGCGAAGCCAAAGGGAAGAAATCGAAGGCCTAA

Protein sequence

MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKINKQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSNDEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQALINYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLCAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGNDSWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQPFMGGKTFLPFKAKPSTSEAKGKKSKA
Homology
BLAST of MC06g1566 vs. ExPASy Swiss-Prot
Match: Q39230 (Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g27470 PE=1 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 6.6e-214
Identity = 357/451 (79.16%), Postives = 410/451 (90.91%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VE+VDE+IKLDKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQ+AQL+I  EDASE I+ TE+NK+ S  K+ E ++    L +KLE +GNLVHDSVPV  
Sbjct: 61  KQVAQLKIKKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDK 120

Query: 121 DEANNAIIRSWGEKLVKP---KLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLN 180
           DEANN +I+ WGEK       KLKNHVDLVELLGIADTK+G+EIAG RGF+LKGDG+ LN
Sbjct: 121 DEANNLVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLN 180

Query: 181 QALINYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAE 240
           QALIN+GL FL+KRG+T LQ PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAE
Sbjct: 181 QALINFGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAE 240

Query: 241 QPLCAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPN 300
           QPLCAYH+++WIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEK+EQFC+T PN
Sbjct: 241 QPLCAYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPN 300

Query: 301 GNDSWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELV 360
            N SW+M +EM+KNSEDFY+ L LPYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELV
Sbjct: 301 ENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELV 360

Query: 361 SCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIP 420
           SCSNCTDYQ+RRLEIRYGQKKSNEQTKQYVH+LNSTLTATERT+CCILENYQ+EDGV+IP
Sbjct: 361 SCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIP 420

Query: 421 EVLQPFMGGKTFLPFKAKPSTSEAKGKKSKA 449
           EVLQPFMGG+TFLPFKAKP  ++ KGKKSKA
Sbjct: 421 EVLQPFMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of MC06g1566 vs. ExPASy Swiss-Prot
Match: O81983 (Serine--tRNA ligase OS=Helianthus annuus OX=4232 PE=2 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 3.6e-212
Identity = 356/435 (81.84%), Postives = 397/435 (91.26%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKG NPEI+RESQRRR A+VE+VDE+IKLDKEWRQRQFELE LRK+FN+IN
Sbjct: 1   MLDINLFREEKGGNPEIVRESQRRRGASVEIVDEIIKLDKEWRQRQFELEQLRKDFNRIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           K++A+LRI+G DAS  IK+TEENK  +A+K  E ++    L SKLE +GNLVHDSVPVSN
Sbjct: 61  KEVAKLRISGGDASSLIKNTEENKDSTAKKQAEVQEARTALYSKLETVGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEA+NA++R+WGE+  +  LKNHV+LVELLGIAD KKG+ +AGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEADNAVVRTWGERREETNLKNHVELVELLGIADLKKGANVAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           IN+GLDFLEKR YTALQTPFFMRKDIM +CAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INFGLDFLEKRKYTALQTPFFMRKDIMGKCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           CAYH++DWIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+T P+GND
Sbjct: 241 CAYHIDDWIHPNELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCLTKPSGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSE+FY+ L LPYQVV+IVSGALNDAAAKKYDLEAWFPAS+TYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEEFYQMLKLPYQVVSIVSGALNDAAAKKYDLEAWFPASKTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSR+LEIR GQKK NEQ KQY HLLNSTLTATERTLCCILENYQ E GV IPEVL
Sbjct: 361 NCTDYQSRKLEIRCGQKKGNEQAKQYCHLLNSTLTATERTLCCILENYQTEKGVAIPEVL 420

Query: 421 QPFMGGKTFLPFKAK 436
           QPFMGGKTFL F  K
Sbjct: 421 QPFMGGKTFLDFLKK 435

BLAST of MC06g1566 vs. ExPASy Swiss-Prot
Match: O14018 (Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29A4.15 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 2.6e-141
Identity = 266/456 (58.33%), Postives = 334/456 (73.25%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLF+ EKG NPEIIRESQR+R A+V +VD+VI++ KEW   +FEL+N  K  N++ 
Sbjct: 1   MLDINLFQVEKGGNPEIIRESQRKRGADVGVVDKVIEMYKEWVSLRFELDNTNKSINRVQ 60

Query: 61  KQIAQLRIAGEDASEKIKD----TEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSV 120
           K+I     A EDASE +++    TE  K L  ++  + K++L  ++S    IGN+VHDSV
Sbjct: 61  KEIGLKMKAKEDASELLEEKNSLTERKKNLIEQETAKNKEMLNVVSS----IGNIVHDSV 120

Query: 121 PVSNDEANNAIIRSWGEKLVKPKLKN---HVDLVELLGIADTKKGSEIAGGRGFYLKGDG 180
           PVS DE NN IIR W  + V  + KN   H +++  L   D ++G +++G RG++L+  G
Sbjct: 121 PVSMDEDNNEIIRKWAPEGVTVEKKNCLSHHEVLTRLDGYDPERGVKVSGHRGYFLRQYG 180

Query: 181 VRLNQALINYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLI 240
           V  N ALI YGLDFLEKRGY ALQ P  + KD+MA+ AQL QFDEELYKV    +++YLI
Sbjct: 181 VFFNLALIQYGLDFLEKRGYIALQAPTMLNKDVMAKTAQLEQFDEELYKVIDGDEERYLI 240

Query: 241 ATAEQPLCAYHLEDWIH-PS-QLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQF 300
           AT+EQP+ AYH  +W   PS QLP++YAGYS+C+R+EAGSHGRD  GIFRVH FEK+EQF
Sbjct: 241 ATSEQPISAYHSGEWFEKPSEQLPLKYAGYSTCYRREAGSHGRDAWGIFRVHAFEKIEQF 300

Query: 301 CMTSPNGNDSWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQ 360
            +T P    SW+   EMI ++EDFYK+L LPY++VAIVSGALN+AAAKKYDLEAWFP   
Sbjct: 301 VLTDP--EKSWEAFTEMINHAEDFYKSLELPYRIVAIVSGALNNAAAKKYDLEAWFPFQG 360

Query: 361 TYRELVSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKE 420
            Y+ELVSCSNCTDYQSR LEIR G KK  ++ K+YVH LNSTL ATER LCCILENYQ  
Sbjct: 361 EYKELVSCSNCTDYQSRNLEIRCGVKKMGDREKKYVHCLNSTLCATERALCCILENYQTP 420

Query: 421 DGVEIPEVLQPFMGGKTFLPF-KAKPSTSEAKGKKS 447
           DGV +P+VLQP+MGGKTFLPF K  P  S +K  K+
Sbjct: 421 DGVNVPKVLQPYMGGKTFLPFTKELPKNSTSKKGKN 450

BLAST of MC06g1566 vs. ExPASy Swiss-Prot
Match: Q7KWQ2 (Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=serS PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.7e-140
Identity = 251/446 (56.28%), Postives = 323/446 (72.42%), Query Frame = 0

Query: 2   LDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKINK 61
           LDI LFR +KG NP++IR+SQ+ R+ANV+ V EVI LDK  ++ ++ L+N   E+ K+NK
Sbjct: 3   LDIVLFRADKGGNPDLIRQSQKVRYANVDAVQEVIDLDKVVKETKYRLDNTNAEYAKLNK 62

Query: 62  QIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSND 121
            +A  + AGE A E I   EE  +   +   E  +V   L  KL  IGN+VH+SVP+ N+
Sbjct: 63  SVAMKKKAGESADEIIAQAEELNQSIIKLKAEVSEVELLLRKKLRPIGNIVHESVPIDNN 122

Query: 122 EANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQALI 181
           E NN II++WGE      L +H +L+E++   D ++G+ ++G R ++LKG GV LNQA+I
Sbjct: 123 EDNNQIIKTWGECKTSEGLLHHHELLEMIDGYDPERGTLVSGHRCYFLKGIGVLLNQAII 182

Query: 182 NYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPLC 241
           N+ L  + KRG   LQTPFFM KD+MA+ AQL QFD+ELYKVTG+ ++KYLIAT+EQP+ 
Sbjct: 183 NFALMHMTKRGSVPLQTPFFMNKDVMAKTAQLEQFDDELYKVTGDNEEKYLIATSEQPIS 242

Query: 242 AYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGNDS 301
           A+H ++WI    LP +Y GYS+CFRKEAGSHGRDT GIFRVHQFEK+EQFC+T P    S
Sbjct: 243 AFHQDEWIEEKDLPKKYVGYSTCFRKEAGSHGRDTWGIFRVHQFEKIEQFCITEP--EKS 302

Query: 302 WDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSN 361
           WDM EEMI NSE FY+ L +PY+VV IVSGALN+AA+KKYDLE WFP    YRELVSCSN
Sbjct: 303 WDMMEEMINNSEQFYQELGIPYRVVNIVSGALNNAASKKYDLEGWFPGYNQYRELVSCSN 362

Query: 362 CTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVLQ 421
           CTDYQSR LEIR G KK  +Q K+YVH+LNSTL AT R +CCILENYQ E G+ +P  L+
Sbjct: 363 CTDYQSRDLEIRCGMKKQGQQQKKYVHMLNSTLAATTRVICCILENYQTEGGITVPVPLR 422

Query: 422 PFMGGKTFLPF-KAKPSTSEAKGKKS 447
           P++ GK F+PF KA P       +K+
Sbjct: 423 PYL-GKDFIPFVKAAPKQKAIPKQKT 445

BLAST of MC06g1566 vs. ExPASy Swiss-Prot
Match: Q9HGT6 (Serine--tRNA ligase, cytoplasmic OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=SES1 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.4e-131
Identity = 257/467 (55.03%), Postives = 324/467 (69.38%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDIN F  EKG +PEII+ SQ++R  +VELVDE+I   KEW + +F+L+   K+ N + 
Sbjct: 1   MLDINAFLVEKGGDPEIIKASQKKRGDSVELVDEIIAEYKEWVKLRFDLDEHNKKLNSVQ 60

Query: 61  KQIAQLRIAGEDASEKIKDTEE---NKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVP 120
           K+I +   A EDA + I + E+    KK   EK+ EA    K L SK+  +GN+VH+SV 
Sbjct: 61  KEIGKRFKAKEDAKDLIAEKEKLSNEKKEIIEKEAEAD---KNLRSKINQVGNIVHESVV 120

Query: 121 VSNDEANNAIIRSW-GEKLVKP-----------KLKNHVDLVELLGIADTKKGSEIAGGR 180
            S DE NN ++R+W  E   KP           KL +H  L+ L G  D ++G  I G R
Sbjct: 121 DSQDEENNELVRTWTPENYKKPEQIAAATGAPAKLSHHEVLLRLDGY-DPERGVRIVGHR 180

Query: 181 GFYLKGDGVRLNQALINYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTG 240
           G++L+  GV LNQALINYGL FL  +GY  LQ P  M K++MA+ AQL+QFDEELYKV  
Sbjct: 181 GYFLRNYGVFLNQALINYGLSFLSSKGYVPLQAPVMMNKEVMAKTAQLSQFDEELYKVID 240

Query: 241 EGDDKYLIATAEQPLCAYHLEDWIH--PSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVH 300
             D+KYLIAT+EQP+ AYH  +W      QLP+RYAGYSSCFR+EAGSHG+D  GIFRVH
Sbjct: 241 GEDEKYLIATSEQPISAYHAGEWFESPAEQLPVRYAGYSSCFRREAGSHGKDAWGIFRVH 300

Query: 301 QFEKVEQFCMTSPNGNDSWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDL 360
            FEK+EQF +T P    SW+  + MI  SE+FY++L LPY+VV IVSG LN+AAAKKYDL
Sbjct: 301 AFEKIEQFVLTEP--EKSWEEFDRMIGCSEEFYQSLGLPYRVVGIVSGELNNAAAKKYDL 360

Query: 361 EAWFPASQTYRELVSCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCC 420
           EAWFP  Q Y+ELVSCSNCTDYQSR LEIR G K+ N+Q K+YVH LNSTL+ATERT+CC
Sbjct: 361 EAWFPFQQEYKELVSCSNCTDYQSRNLEIRCGIKQQNQQEKKYVHCLNSTLSATERTICC 420

Query: 421 ILENYQKEDGVEIPEVLQPFMGGK-TFLPF-----KAKPSTSEAKGK 445
           ILENYQKEDG+ IPEVL+ ++ G+  F+P+     K   S  +AKGK
Sbjct: 421 ILENYQKEDGLVIPEVLRKYIPGEPEFIPYIKELPKNTTSVKKAKGK 461

BLAST of MC06g1566 vs. NCBI nr
Match: XP_022145958.1 (serine--tRNA ligase [Momordica charantia] >XP_022145959.1 serine--tRNA ligase [Momordica charantia])

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL
Sbjct: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND
Sbjct: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTFLPFKAKPSTSEAKGKKSKA
Sbjct: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448

BLAST of MC06g1566 vs. NCBI nr
Match: XP_023530248.1 (serine--tRNA ligase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870 bits (2249), Expect = 0.0
Identity = 432/448 (96.43%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNA+IRSWGEKLVKPKLKNHVDLVELLGIAD+KKGSEIAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTA+QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTF+PFKAKP+TS+AKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of MC06g1566 vs. NCBI nr
Match: XP_022926250.1 (serine--tRNA ligase [Cucurbita moschata])

HSP 1 Score: 869 bits (2246), Expect = 0.0
Identity = 431/448 (96.21%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNA+IRSWGEKLVKPKLKNHVDLVELLGIAD+KKGSEIAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTA+QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTF+PFKAKP+TS+AKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of MC06g1566 vs. NCBI nr
Match: XP_022969645.1 (serine--tRNA ligase [Cucurbita maxima])

HSP 1 Score: 868 bits (2244), Expect = 0.0
Identity = 431/448 (96.21%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNA+IRSWGEKLVKPKLKNHVDLVELLGIAD+KKGSEIAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTA+QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPAS TYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASHTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTF+PFKAKP+TS+AKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of MC06g1566 vs. NCBI nr
Match: KAG7022303.1 (Serine--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 868 bits (2242), Expect = 0.0
Identity = 430/448 (95.98%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNA+IRSWGEKLVKPKLKNHVDLVELLGIAD+KKGSEIAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTA+QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SW+MHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWEMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTF+PFKAKP+TS+AKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of MC06g1566 vs. ExPASy TrEMBL
Match: A0A6J1CWS6 (Seryl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015292 PE=4 SV=1)

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL
Sbjct: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND
Sbjct: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTFLPFKAKPSTSEAKGKKSKA
Sbjct: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448

BLAST of MC06g1566 vs. ExPASy TrEMBL
Match: A0A6J1EKK7 (Seryl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433427 PE=4 SV=1)

HSP 1 Score: 869 bits (2246), Expect = 0.0
Identity = 431/448 (96.21%), Postives = 447/448 (99.78%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVI+LDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIRLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNA+IRSWGEKLVKPKLKNHVDLVELLGIAD+KKGSEIAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTA+QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTF+PFKAKP+TS+AKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of MC06g1566 vs. ExPASy TrEMBL
Match: A0A6J1I0I1 (Seryl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468610 PE=4 SV=1)

HSP 1 Score: 868 bits (2244), Expect = 0.0
Identity = 431/448 (96.21%), Postives = 446/448 (99.55%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANNA+IRSWGEKLVKPKLKNHVDLVELLGIAD+KKGSEIAGGRG+YLKGDGVRLNQAL
Sbjct: 121 DEANNAVIRSWGEKLVKPKLKNHVDLVELLGIADSKKGSEIAGGRGYYLKGDGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYTA+QTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQP+
Sbjct: 181 INYGLDFLEKRGYTAMQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPI 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLPIRYAGYS+CFRKEAGSHGRDTLGIFRVHQFEK+EQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPIRYAGYSTCFRKEAGSHGRDTLGIFRVHQFEKIEQFCVTSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPAS TYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASHTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLE R+GQKKSNEQTKQYVH+LNSTLTATERTLCCILENYQKEDGVEIPEVL
Sbjct: 361 NCTDYQSRRLETRFGQKKSNEQTKQYVHMLNSTLTATERTLCCILENYQKEDGVEIPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           QPFMGGKTF+PFKAKP+TS+AKGKKSKA
Sbjct: 421 QPFMGGKTFMPFKAKPTTSDAKGKKSKA 448

BLAST of MC06g1566 vs. ExPASy TrEMBL
Match: A0A1S3CM54 (Seryl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103502550 PE=4 SV=1)

HSP 1 Score: 854 bits (2206), Expect = 3.86e-312
Identity = 423/448 (94.42%), Postives = 441/448 (98.44%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASE+IKDTEENK+LSAEKDVEAKDVLK+LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANN +IRSWGEKLVKPKLKNHVDLVELL IADTK+GSEIAGGRG+YLKG GVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           +P++GGK FLPFK KP+ +++KGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of MC06g1566 vs. ExPASy TrEMBL
Match: A0A5D3CBX2 (Seryl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold76G00430 PE=4 SV=1)

HSP 1 Score: 854 bits (2206), Expect = 3.86e-312
Identity = 423/448 (94.42%), Postives = 441/448 (98.44%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQIAQLRIAGEDASE+IKDTEENK+LSAEKDVEAKDVLK+LNSKLEIIGNLVHDSVPVSN
Sbjct: 61  KQIAQLRIAGEDASERIKDTEENKRLSAEKDVEAKDVLKKLNSKLEIIGNLVHDSVPVSN 120

Query: 121 DEANNAIIRSWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQAL 180
           DEANN +IRSWGEKLVKPKLKNHVDLVELL IADTK+GSEIAGGRG+YLKG GVRLNQAL
Sbjct: 121 DEANNVVIRSWGEKLVKPKLKNHVDLVELLEIADTKRGSEIAGGRGYYLKGAGVRLNQAL 180

Query: 181 INYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240
           INYGLDFLEKRGYT LQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL
Sbjct: 181 INYGLDFLEKRGYTELQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAEQPL 240

Query: 241 CAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGND 300
           C+YHLEDWIHPSQLP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFC+TSPNGND
Sbjct: 241 CSYHLEDWIHPSQLPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCITSPNGND 300

Query: 301 SWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360
           SWDMHEEMIKNSEDFYKTLN+PYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS
Sbjct: 301 SWDMHEEMIKNSEDFYKTLNIPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCS 360

Query: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIPEVL 420
           NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVE+PEVL
Sbjct: 361 NCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEVPEVL 420

Query: 421 QPFMGGKTFLPFKAKPSTSEAKGKKSKA 448
           +P++GGK FLPFK KP+ +++KGKKSKA
Sbjct: 421 RPYLGGKAFLPFKTKPTVNDSKGKKSKA 448

BLAST of MC06g1566 vs. TAIR 10
Match: AT5G27470.1 (seryl-tRNA synthetase / serine--tRNA ligase )

HSP 1 Score: 744.6 bits (1921), Expect = 4.7e-215
Identity = 357/451 (79.16%), Postives = 410/451 (90.91%), Query Frame = 0

Query: 1   MLDINLFREEKGNNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKIN 60
           MLDINLFREEKGNNPEIIRESQRRRFA+VE+VDE+IKLDKEWRQRQFE+++ RKEFNK+N
Sbjct: 1   MLDINLFREEKGNNPEIIRESQRRRFASVEIVDEIIKLDKEWRQRQFEVDSFRKEFNKLN 60

Query: 61  KQIAQLRIAGEDASEKIKDTEENKKLSAEKDVEAKDVLKQLNSKLEIIGNLVHDSVPVSN 120
           KQ+AQL+I  EDASE I+ TE+NK+ S  K+ E ++    L +KLE +GNLVHDSVPV  
Sbjct: 61  KQVAQLKIKKEDASEIIQQTEKNKQDSTAKEAEVREAYAALKAKLEQVGNLVHDSVPVDK 120

Query: 121 DEANNAIIRSWGEKLVKP---KLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLN 180
           DEANN +I+ WGEK       KLKNHVDLVELLGIADTK+G+EIAG RGF+LKGDG+ LN
Sbjct: 121 DEANNLVIKLWGEKRFSTPGLKLKNHVDLVELLGIADTKRGAEIAGARGFFLKGDGLMLN 180

Query: 181 QALINYGLDFLEKRGYTALQTPFFMRKDIMARCAQLAQFDEELYKVTGEGDDKYLIATAE 240
           QALIN+GL FL+KRG+T LQ PFFMRKD+MA+CAQLAQFDEELYKVTGEGDDKYLIATAE
Sbjct: 181 QALINFGLTFLKKRGFTGLQPPFFMRKDVMAKCAQLAQFDEELYKVTGEGDDKYLIATAE 240

Query: 241 QPLCAYHLEDWIHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPN 300
           QPLCAYH+++WIHP++LP+RYAGYSSCFRKEAGSHGRDTLGIFRVHQFEK+EQFC+T PN
Sbjct: 241 QPLCAYHIDEWIHPTELPLRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKIEQFCITGPN 300

Query: 301 GNDSWDMHEEMIKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELV 360
            N SW+M +EM+KNSEDFY+ L LPYQ+V+IVSGALNDAAAKKYDLEAWFP+S+T+RELV
Sbjct: 301 ENASWEMLDEMMKNSEDFYQALKLPYQIVSIVSGALNDAAAKKYDLEAWFPSSETFRELV 360

Query: 361 SCSNCTDYQSRRLEIRYGQKKSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDGVEIP 420
           SCSNCTDYQ+RRLEIRYGQKKSNEQTKQYVH+LNSTLTATERT+CCILENYQ+EDGV+IP
Sbjct: 361 SCSNCTDYQARRLEIRYGQKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDGVDIP 420

Query: 421 EVLQPFMGGKTFLPFKAKPSTSEAKGKKSKA 449
           EVLQPFMGG+TFLPFKAKP  ++ KGKKSKA
Sbjct: 421 EVLQPFMGGETFLPFKAKPVVADTKGKKSKA 451

BLAST of MC06g1566 vs. TAIR 10
Match: AT1G11870.2 (Seryl-tRNA synthetase )

HSP 1 Score: 249.6 bits (636), Expect = 4.7e-66
Identity = 157/438 (35.84%), Postives = 242/438 (55.25%), Query Frame = 0

Query: 13  NNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKINKQIAQLRIAGED 72
           +N E +  + R R +N  L + V++L +     Q E+E LR+E N + K++        +
Sbjct: 86  DNKEAVEINIRNRNSNANL-EAVLQLYENMVNLQKEVERLREERNNVAKKMK----GKLE 145

Query: 73  ASEKIKDTEENKKLSAEKDVEAKDVLK---QLNSKLEIIGNLVHDSVPVSNDEANNAIIR 132
            SE+ +  EE K L        +D++K   +L    + I N+ H  VPV  ++++     
Sbjct: 146 PSERERLVEEGKNLKESLVTLEEDLVKLKDELQHVAQSIPNMTHPDVPVGGEDSSAIRQE 205

Query: 133 SWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQALINYGLDFLE 192
               +     +K+H+ L + L + D    +E++G + FYLK + V L  AL+N+ L  + 
Sbjct: 206 VGSPREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALLNWTLSQVM 265

Query: 193 KRGYTALQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQPLCAYHLEDW 252
           K+GYT L TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE P+   H++  
Sbjct: 266 KKGYTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPVGGIHMDSI 325

Query: 253 IHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGNDSWDMHEEM 312
           +  S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F +  P   DS   H+E+
Sbjct: 326 LLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQP--EDSESFHQEL 385

Query: 313 IKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQSR 372
           I+  ED + +L L ++ + + +  L   A +K+D+EAW P    + E+ S SNCTDYQSR
Sbjct: 386 IQIEEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSR 445

Query: 373 RLEIRY--------GQK--KSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDG-VEIP 432
           RL IRY        G K  K+N    ++VH LN+T  A  R + C+LENYQ+EDG V IP
Sbjct: 446 RLGIRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGSVVIP 505

Query: 433 EVLQPFMGGKTFLPFKAK 436
           E L+PFMGG   +  K K
Sbjct: 506 EPLRPFMGGIELIKPKHK 514

BLAST of MC06g1566 vs. TAIR 10
Match: AT1G11870.1 (Seryl-tRNA synthetase )

HSP 1 Score: 249.6 bits (636), Expect = 4.7e-66
Identity = 157/438 (35.84%), Postives = 242/438 (55.25%), Query Frame = 0

Query: 13  NNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKINKQIAQLRIAGED 72
           +N E +  + R R +N  L + V++L +     Q E+E LR+E N + K++        +
Sbjct: 84  DNKEAVEINIRNRNSNANL-EAVLQLYENMVNLQKEVERLREERNNVAKKMK----GKLE 143

Query: 73  ASEKIKDTEENKKLSAEKDVEAKDVLK---QLNSKLEIIGNLVHDSVPVSNDEANNAIIR 132
            SE+ +  EE K L        +D++K   +L    + I N+ H  VPV  ++++     
Sbjct: 144 PSERERLVEEGKNLKESLVTLEEDLVKLKDELQHVAQSIPNMTHPDVPVGGEDSSAIRQE 203

Query: 133 SWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQALINYGLDFLE 192
               +     +K+H+ L + L + D    +E++G + FYLK + V L  AL+N+ L  + 
Sbjct: 204 VGSPREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALLNWTLSQVM 263

Query: 193 KRGYTALQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQPLCAYHLEDW 252
           K+GYT L TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE P+   H++  
Sbjct: 264 KKGYTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPVGGIHMDSI 323

Query: 253 IHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGNDSWDMHEEM 312
           +  S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F +  P   DS   H+E+
Sbjct: 324 LLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQP--EDSESFHQEL 383

Query: 313 IKNSEDFYKTLNLPYQVVAIVSGALNDAAAKKYDLEAWFPASQTYRELVSCSNCTDYQSR 372
           I+  ED + +L L ++ + + +  L   A +K+D+EAW P    + E+ S SNCTDYQSR
Sbjct: 384 IQIEEDLFTSLGLHFKTLDMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSR 443

Query: 373 RLEIRY--------GQK--KSNEQTKQYVHLLNSTLTATERTLCCILENYQKEDG-VEIP 432
           RL IRY        G K  K+N    ++VH LN+T  A  R + C+LENYQ+EDG V IP
Sbjct: 444 RLGIRYRPSEPPQTGPKKGKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGSVVIP 503

Query: 433 EVLQPFMGGKTFLPFKAK 436
           E L+PFMGG   +  K K
Sbjct: 504 EPLRPFMGGIELIKPKHK 512

BLAST of MC06g1566 vs. TAIR 10
Match: AT1G11870.3 (Seryl-tRNA synthetase )

HSP 1 Score: 156.4 bits (394), Expect = 5.5e-38
Identity = 101/315 (32.06%), Postives = 168/315 (53.33%), Query Frame = 0

Query: 13  NNPEIIRESQRRRFANVELVDEVIKLDKEWRQRQFELENLRKEFNKINKQIAQLRIAGED 72
           +N E +  + R R +N  L + V++L +     Q E+E LR+E N + K++        +
Sbjct: 86  DNKEAVEINIRNRNSNANL-EAVLQLYENMVNLQKEVERLREERNNVAKKMK----GKLE 145

Query: 73  ASEKIKDTEENKKLSAEKDVEAKDVLK---QLNSKLEIIGNLVHDSVPVSNDEANNAIIR 132
            SE+ +  EE K L        +D++K   +L    + I N+ H  VPV  ++++     
Sbjct: 146 PSERERLVEEGKNLKESLVTLEEDLVKLKDELQHVAQSIPNMTHPDVPVGGEDSSAIRQE 205

Query: 133 SWGEKLVKPKLKNHVDLVELLGIADTKKGSEIAGGRGFYLKGDGVRLNQALINYGLDFLE 192
               +     +K+H+ L + L + D    +E++G + FYLK + V L  AL+N+ L  + 
Sbjct: 206 VGSPREFSFPIKDHLQLGKDLDLIDFDSAAEVSGSKFFYLKNEAVLLEMALLNWTLSQVM 265

Query: 193 KRGYTALQTPFFMRKDIMARCAQLAQFDE-ELYKVTGEGDDKYLIATAEQPLCAYHLEDW 252
           K+GYT L TP  +R  I+ +C    + D  ++Y +  +G D+ LI TAE P+   H++  
Sbjct: 266 KKGYTPLTTPEIVRSSIVEKCGFQPRGDNTQVYSI--DGTDQCLIGTAEIPVGGIHMDSI 325

Query: 253 IHPSQLPIRYAGYSSCFRKEAGSHGRDTLGIFRVHQFEKVEQFCMTSPNGNDSWDMHEEM 312
           +  S LP++Y  +S CFR EAG+ G  T G++RVHQF K E F +  P   DS   H+E+
Sbjct: 326 LLESALPLKYIAFSHCFRTEAGAAGAATKGLYRVHQFSKAEMFVICQP--EDSESFHQEL 385

Query: 313 IKNSEDFYKTLNLPY 324
           I+  ED + +L L +
Sbjct: 386 IQIEEDLFTSLGLHF 391

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q392306.6e-21479.16Serine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At5g27470 PE... [more]
O819833.6e-21281.84Serine--tRNA ligase OS=Helianthus annuus OX=4232 PE=2 SV=1[more]
O140182.6e-14158.33Serine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q7KWQ21.7e-14056.28Serine--tRNA ligase, cytoplasmic OS=Dictyostelium discoideum OX=44689 GN=serS PE... [more]
Q9HGT61.4e-13155.03Serine--tRNA ligase, cytoplasmic OS=Candida albicans (strain SC5314 / ATCC MYA-2... [more]
Match NameE-valueIdentityDescription
XP_022145958.10.0100.00serine--tRNA ligase [Momordica charantia] >XP_022145959.1 serine--tRNA ligase [M... [more]
XP_023530248.10.096.43serine--tRNA ligase [Cucurbita pepo subsp. pepo][more]
XP_022926250.10.096.21serine--tRNA ligase [Cucurbita moschata][more]
XP_022969645.10.096.21serine--tRNA ligase [Cucurbita maxima][more]
KAG7022303.10.095.98Serine--tRNA ligase, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1CWS60.0100.00Seryl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111015292 PE=4 SV=1[more]
A0A6J1EKK70.096.21Seryl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111433427 PE=4 SV=1[more]
A0A6J1I0I10.096.21Seryl-tRNA synthetase OS=Cucurbita maxima OX=3661 GN=LOC111468610 PE=4 SV=1[more]
A0A1S3CM543.86e-31294.42Seryl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103502550 PE=4 SV=1[more]
A0A5D3CBX23.86e-31294.42Seryl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold76... [more]
Match NameE-valueIdentityDescription
AT5G27470.14.7e-21579.16seryl-tRNA synthetase / serine--tRNA ligase [more]
AT1G11870.24.7e-6635.84Seryl-tRNA synthetase [more]
AT1G11870.14.7e-6635.84Seryl-tRNA synthetase [more]
AT1G11870.35.5e-3832.06Seryl-tRNA synthetase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 49..83
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 110..440
e-value: 1.4E-145
score: 486.5
NoneNo IPR availablePANTHERPTHR11778:SF18BNAA09G03770D PROTEINcoord: 1..433
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 122..431
IPR002317Serine-tRNA ligase, type1PRINTSPR00981TRNASYNTHSERcoord: 357..373
score: 67.76
coord: 339..355
score: 61.66
coord: 280..293
score: 63.23
coord: 268..280
score: 66.38
coord: 322..335
score: 45.24
IPR002317Serine-tRNA ligase, type1PIRSFPIRSF001529Ser-tRNA_ligasecoord: 1..433
e-value: 2.0E-186
score: 617.7
IPR002317Serine-tRNA ligase, type1TIGRFAMTIGR00414TIGR00414coord: 1..424
e-value: 1.5E-147
score: 489.9
IPR002317Serine-tRNA ligase, type1PANTHERPTHR11778SERYL-TRNA SYNTHETASEcoord: 1..433
IPR042103Serine-tRNA synthetase, type1, N-terminal domain superfamilyGENE3D1.10.287.40coord: 1..109
e-value: 8.9E-38
score: 131.1
IPR002314Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)PFAMPF00587tRNA-synt_2bcoord: 221..408
e-value: 2.3E-36
score: 125.5
IPR015866Serine-tRNA synthetase, type1, N-terminalPFAMPF02403Seryl_tRNA_Ncoord: 1..112
e-value: 1.3E-25
score: 89.7
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 142..417
score: 21.743256
IPR033729Serine-tRNA ligase catalytic core domainCDDcd00770SerRS_corecoord: 124..424
e-value: 0.0
score: 519.809
IPR010978Class I and II aminoacyl-tRNA synthetase, tRNA-binding armSUPERFAMILY46589tRNA-binding armcoord: 1..117

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1566.1MC06g1566.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006434 seryl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0004828 serine-tRNA ligase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding