MC06g1531 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g1531 vs. ExPASy Swiss-Prot
Match: Q9ZP54 (Poly [ADP-ribose] polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PARP1 PE=1 SV=2)

HSP 1 Score: 1357.8 bits (3513), Expect = 0.0e+00
Identity = 683/995 (68.64%), Postives = 807/995 (81.11%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MA+P KPW+ EYAKSSRSSCKTCK  I+KEN R GK+VQ+T FDG MPMWNHASCILKK 
Sbjct: 1   MASPHKPWRAEYAKSSRSSCKTCKSVINKENFRLGKLVQSTHFDGIMPMWNHASCILKKT 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADT-----------PVECGIEVAQ 120
           KQIKSVDDVEG++SLRWEDQQKIRKYVE  A +     T            +E GIEV+Q
Sbjct: 61  KQIKSVDDVEGIESLRWEDQQKIRKYVESGAGSNTSTSTGTSTSSTANNAKLEYGIEVSQ 120

Query: 121 ASRAACKLCKQKIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPA 180
            SRA C+ C +KI+KGEVR+ +  +G G KGL WHHA C++E S ST++E L+GW+++P 
Sbjct: 121 TSRAGCRKCSEKILKGEVRIFSKPEGPGNKGLMWHHAKCFLEMSSSTELESLSGWRSIPD 180

Query: 181 SDQAAVSALIKK-LPS--TENNEPRQAASKVG-KRKKDATGDQDSKVTKAVGDVSVSRSM 240
           SDQ A+  L+KK LP+  TE  E RQ  S+ G KRK D+  ++ SK+ K+  D+S S ++
Sbjct: 181 SDQEALLPLVKKALPAAKTETAEARQTNSRAGTKRKNDSVDNEKSKLAKSSFDMSTSGAL 240

Query: 241 KTASDSPDSQNPSSLASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDL 300
           +          P S   ++EAQTKELW LKDDLKK+VT+AELREMLE N Q + GSELDL
Sbjct: 241 Q----------PCSKEKEMEAQTKELWDLKDDLKKYVTSAELREMLEVNEQSTRGSELDL 300

Query: 301 RDRCADGMMFGALANCPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPE 360
           RD+CADGMMFG LA CP+CS  L +SGG+YRCHGY S WSKCS+S  +P+RIKGKWK+P+
Sbjct: 301 RDKCADGMMFGPLALCPMCSGHLSFSGGLYRCHGYISEWSKCSHSTLDPDRIKGKWKIPD 360

Query: 361 ETGNQYLCKWFKSQKVAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLK 420
           ET NQ+L KW KSQK  KP R+L P  S   +  Q S   + SS++E LA+L+V+ +G  
Sbjct: 361 ETENQFLLKWNKSQKSVKPKRILRPVLSGETSQGQGSKDATDSSRSERLADLKVSIAGNT 420

Query: 421 DSMEEWKRKIEGEGGAVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVEC 480
              + WK++IE  G   HA +KK T+CLVV G  D  + E RKAR+MK+ IVRE+YLV+C
Sbjct: 421 KERQPWKKRIEEAGAEFHANVKKGTSCLVVCGLTDIRDAEMRKARRMKVAIVREDYLVDC 480

Query: 481 FRKQKKLPFDRYKVETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNM 540
           F+KQ+KLPFD+YK+E T E  S+VTVKVKGRSAVHE+SGLQ+  HILED  SIYNTTL+M
Sbjct: 481 FKKQRKLPFDKYKIEDTSE--SLVTVKVKGRSAVHEASGLQEHCHILEDGNSIYNTTLSM 540

Query: 541 SDLSTGVNSYYILQIIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFL 600
           SDLSTG+NSYYILQIIQ+DK SDC+VFRKWGRVGNEKIGG K+EEMSKSDA+ EFKRLFL
Sbjct: 541 SDLSTGINSYYILQIIQEDKGSDCYVFRKWGRVGNEKIGGNKVEEMSKSDAVHEFKRLFL 600

Query: 601 EKTGNPWEAWEQKQNFEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLF 660
           EKTGN WE+WEQK NF+KQPG+F PLDIDYGVNK + KK+   +++ LAP L ELMKMLF
Sbjct: 601 EKTGNTWESWEQKTNFQKQPGKFLPLDIDYGVNKQVAKKEPFQTSSNLAPSLIELMKMLF 660

Query: 661 NVETYRNAMMEFEINMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDA 720
           +VETYR+AMMEFEINMSEMPLGKLS+ NIQKGFEALTEIQ LL  S    +MKESL++DA
Sbjct: 661 DVETYRSAMMEFEINMSEMPLGKLSKHNIQKGFEALTEIQRLLTESDPQPTMKESLLVDA 720

Query: 721 SNRFFTVIPSIHPHIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLR 780
           SNRFFT+IPSIHPHIIRDEDDFKSK+KMLEALQDIEIASR+VGFD D+ ESLD+KYKKL 
Sbjct: 721 SNRFFTMIPSIHPHIIRDEDDFKSKVKMLEALQDIEIASRIVGFDVDSTESLDDKYKKLH 780

Query: 781 CDIAPISHESEDYKLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENK 840
           CDI+P+ H+SEDY+LIEKYL TTHAPTH +W LELEEVF+LEREGE+DK+AP R KL NK
Sbjct: 781 CDISPLPHDSEDYRLIEKYLNTTHAPTHTEWSLELEEVFALEREGEFDKYAPHREKLGNK 840

Query: 841 MLLWHGSRLTNFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVG 900
           MLLWHGSRLTNFVGIL+QGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYT +KNPVG
Sbjct: 841 MLLWHGSRLTNFVGILNQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTCKKNPVG 900

Query: 901 FMVLSEVALGEVYEVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVA 960
            M+LSEVALGE++E+ KA+YM++PPRGKHSTKG+GKKVP  SE  KWR DV VPCGKPV+
Sbjct: 901 LMLLSEVALGEIHELTKAKYMDKPPRGKHSTKGLGKKVPQDSEFAKWRGDVTVPCGKPVS 960

Query: 961 SNVKASELMYNEYIVYNTAQVKMQFLLKVRFHHKR 981
           S VKASELMYNEYIVY+TAQVK+QFLLKVRF HKR
Sbjct: 961 SKVKASELMYNEYIVYDTAQVKLQFLLKVRFKHKR 983

BLAST of MC06g1531 vs. ExPASy Swiss-Prot
Match: Q7EYV7 (Poly [ADP-ribose] polymerase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PARP1 PE=2 SV=1)

HSP 1 Score: 1246.1 bits (3223), Expect = 0.0e+00
Identity = 633/992 (63.81%), Postives = 767/992 (77.32%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MA P K WK EYAKS RSSCK+C+ PI K+ LR GKMVQATQFDG MPMWNHASCIL K 
Sbjct: 1   MAAPPKAWKAEYAKSGRSSCKSCRSPIGKDQLRLGKMVQATQFDGLMPMWNHASCILSKK 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAA----AAAVADTPVECGIEVAQASRAACK 120
            QIKSVDDVEG+D+LRW+DQ+KIR YV  + A    AAA++D   +C IEVA+++R +C+
Sbjct: 61  NQIKSVDDVEGIDTLRWDDQEKIRNYVGSAPATASSAAAISD---KCTIEVAKSARTSCR 120

Query: 121 LCKQKIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVS 180
            C +KI KG VR+S+ L+G+G     W+HA+C++E SP+  VE  +GW+ L   D+ AV 
Sbjct: 121 RCGEKIKKGTVRVSSKLEGQG-----WYHASCFLEMSPAATVENFSGWEILSHEDKRAVL 180

Query: 181 ALIKK-LPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSM---KTASDSP 240
            L+KK  PS+      Q +SK  KRK +     D K  K +  +S   +    K      
Sbjct: 181 DLVKKDAPSS-----GQTSSKGSKRKNNQNDIHDCKAPKIIRSISEGTAEDKGKAVVSHD 240

Query: 241 DSQNPSSLASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADG 300
            + N S L  KL+ Q+  LWKLKD+LKKHV+ AELR MLE+NGQD++G E  L DRCADG
Sbjct: 241 SNANSSDLQEKLKEQSDTLWKLKDELKKHVSTAELRNMLEANGQDTSGPERHLLDRCADG 300

Query: 301 MMFGALANCPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYL 360
           M+FGAL  CP+CS+ L Y GG Y C GY S WSKC+YS  EP R K KWK+P+E  N YL
Sbjct: 301 MLFGALGTCPVCSSFLYYHGGQYHCSGYVSEWSKCTYSTTEPVRSKKKWKIPDEMDNGYL 360

Query: 361 CKWFKSQKVAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSG-LKDSMEEW 420
            KWFKSQK  KP R+LPP S   +       +RS  S  E L +LRV+  G  KD ++ W
Sbjct: 361 TKWFKSQKAKKPERVLPPMSPEKSLCQSTQQNRSFLS--EGLDKLRVSIVGQSKDVVDGW 420

Query: 421 KRKIEGEGGAVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKK 480
           K+K++  G   +A + KD++CLV+  +++  N E +KAR++K+PI+RE YL EC RK + 
Sbjct: 421 KQKLKDAGANFNATVTKDSSCLVLCSELESENAEVKKARRLKIPILREGYLGECIRKNRV 480

Query: 481 LPFDRYKVETTGEST--SMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLS 540
           LPFD YKVE   ES+    +TVKVKGRSAVHESSGLQDTGHILED KSIYNTTLNMSDL+
Sbjct: 481 LPFDLYKVEAALESSKGGTMTVKVKGRSAVHESSGLQDTGHILEDGKSIYNTTLNMSDLT 540

Query: 541 TGVNSYYILQIIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTG 600
            GVNSYYILQ+I++D  SDC+VFRKWGRVGNEKIGG KLEEMSK  AI+EF+RLFLEKTG
Sbjct: 541 RGVNSYYILQVIEEDNGSDCYVFRKWGRVGNEKIGGTKLEEMSKIHAIQEFRRLFLEKTG 600

Query: 601 NPWEAWEQKQNFEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVET 660
           NPWEAWEQK NF+KQPG+F+PLDIDYGV +   +K  +   + L PQL ELM MLFN+ET
Sbjct: 601 NPWEAWEQKTNFQKQPGKFYPLDIDYGVRQGPKRKDIDKMKSSLPPQLLELMNMLFNIET 660

Query: 661 YRNAMMEFEINMSEMPLGKLSRSNIQKGFEALTEIQNLL-NSSIHDGSMKESLIIDASNR 720
           YR AM+EF+INMSEMPLGKLS+ NIQKGFEALTEIQNLL N++  + +++ESLI+ ASNR
Sbjct: 661 YRAAMLEFKINMSEMPLGKLSKENIQKGFEALTEIQNLLGNTNNQELAVRESLIVAASNR 720

Query: 721 FFTVIPSIHPHIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDI 780
           FFT+IPSIHPHII+DEDD   K+KMLEALQDIEIAS+LVGFD DN+ESLD+KYKKLRC I
Sbjct: 721 FFTLIPSIHPHIIQDEDDLMVKVKMLEALQDIEIASKLVGFDSDNDESLDDKYKKLRCAI 780

Query: 781 APISHESEDYKLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLL 840
            P+ H+ EDYKL+EKYL+ THAPTH +W LELEEVFSL+R+GE+ K++ ++N L NKMLL
Sbjct: 781 TPLPHDCEDYKLVEKYLLNTHAPTHKEWSLELEEVFSLDRDGEFSKYSRYKNNLHNKMLL 840

Query: 841 WHGSRLTNFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMV 900
           WHGSRLTN+VGILSQGLRIAPPEAP TGYMFGKG+YFADLVSKSAQYCY DRKNPVG M+
Sbjct: 841 WHGSRLTNYVGILSQGLRIAPPEAPVTGYMFGKGLYFADLVSKSAQYCYVDRKNPVGLML 900

Query: 901 LSEVALGEVYEVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNV 960
           LSEVALG++YE+KKA  M++PPRGKHSTKG+GK VP  SE  KWR+DVVVPCGKPV +++
Sbjct: 901 LSEVALGDMYELKKATSMDKPPRGKHSTKGLGKTVPLESEFAKWRDDVVVPCGKPVPASI 960

Query: 961 KASELMYNEYIVYNTAQVKMQFLLKVRFHHKR 981
           K SELMYNEYIVYNT+QVKMQ+LLKVRFHHKR
Sbjct: 961 KTSELMYNEYIVYNTSQVKMQYLLKVRFHHKR 977

BLAST of MC06g1531 vs. ExPASy Swiss-Prot
Match: Q9ZSV1 (Poly [ADP-ribose] polymerase 1 OS=Zea mays OX=4577 GN=PARP1 PE=2 SV=1)

HSP 1 Score: 1232.2 bits (3187), Expect = 0.0e+00
Identity = 620/985 (62.94%), Postives = 754/985 (76.55%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MA P K WK EYAKS R+SCK+C+ PI K+ LR GKMVQA+QFDGFMPMWNHA CI  K 
Sbjct: 1   MAAPPKAWKAEYAKSGRASCKSCRSPIAKDQLRLGKMVQASQFDGFMPMWNHARCIFSKK 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYV-EDSAAAAAVADTPVECGIEVAQASRAACKLCK 120
            QIKSVDDVEG+D+LRW+DQ+KIR YV   SA  ++ A  P +C IE+A ++R +C+ C 
Sbjct: 61  NQIKSVDDVEGIDALRWDDQEKIRNYVGSASAGTSSTAAPPEKCTIEIAPSARTSCRRCS 120

Query: 121 QKIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALI 180
           +KI KG VRLS  L+ +G KG+ W+HANC+ E SPS  VEK +GW  L   D+  +  L+
Sbjct: 121 EKITKGSVRLSAKLESEGPKGIPWYHANCFFEVSPSATVEKFSGWDTLSDEDKRTMLDLV 180

Query: 181 KKLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSS 240
           KK     NNE +   SK  K + D    + +++ ++  + +V    +       + + + 
Sbjct: 181 KK--DVGNNE-QNKGSKRKKSENDIDSYKSARLDESTSEGTVRNKGQLVDPRGSNTSSAD 240

Query: 241 LASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALA 300
           +  KL+ Q+  LWKLKD LK HV+ AELR+MLE+NGQD++G E  L DRCADGM+FGAL 
Sbjct: 241 IQLKLKEQSDTLWKLKDGLKTHVSAAELRDMLEANGQDTSGPERHLLDRCADGMLFGALG 300

Query: 301 NCPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQ 360
            CP+C+  + Y  G Y+C G  S WSKC+YSA EP R+K KW++P  T N YL KWFKSQ
Sbjct: 301 PCPVCANGMYYYNGQYQCSGNVSEWSKCTYSATEPVRVKKKWQIPHGTKNDYLMKWFKSQ 360

Query: 361 KVAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSG-LKDSMEEWKRKIEGE 420
           KV KP R+LPP S   +       +   SSK   L +LR +  G  K++  EW  K++  
Sbjct: 361 KVKKPERVLPPMSPEKSGSKATQRTSLLSSK--GLDKLRFSVVGQSKEAANEWIEKLKLA 420

Query: 421 GGAVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYK 480
           G   +A++ KD +CL+  G++D  N E RKAR++K+PIVRE Y+ EC +K K LPFD YK
Sbjct: 421 GANFYARVVKDIDCLIACGELDNENAEVRKARRLKIPIVREGYIGECVKKNKMLPFDLYK 480

Query: 481 VETTGEST--SMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYY 540
           +E   ES+  S VTVKVKGRSAVHESSGLQDT HILED KSIYN TLNMSDL+ GVNSYY
Sbjct: 481 LENALESSKGSTVTVKVKGRSAVHESSGLQDTAHILEDGKSIYNATLNMSDLALGVNSYY 540

Query: 541 ILQIIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWE 600
           +LQII+ D  S+C+VFRKWGRVG+EKIGG KLEEMSK++AI+EFKRLFLEKTGN WEAWE
Sbjct: 541 VLQIIEQDDGSECYVFRKWGRVGSEKIGGQKLEEMSKTEAIKEFKRLFLEKTGNSWEAWE 600

Query: 601 QKQNFEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMME 660
            K NF KQPGRF+PLD+DYGV K   +K  +   + LAPQL ELMKMLFNVETYR AMME
Sbjct: 601 CKTNFRKQPGRFYPLDVDYGVKKAPKRKDISEMKSSLAPQLLELMKMLFNVETYRAAMME 660

Query: 661 FEINMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDG-SMKESLIIDASNRFFTVIPS 720
           FEINMSEMPLGKLS+ NI+KGFEALTEIQNLL  +     +++ESLI+ ASNRFFT+IPS
Sbjct: 661 FEINMSEMPLGKLSKENIEKGFEALTEIQNLLKDTADQALAVRESLIVAASNRFFTLIPS 720

Query: 721 IHPHIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHES 780
           IHPHIIRDEDD   K KMLEALQDIEIAS++VGFD D++ESLD+KY KL CDI P++H+S
Sbjct: 721 IHPHIIRDEDDLMIKAKMLEALQDIEIASKIVGFDSDSDESLDDKYMKLHCDITPLAHDS 780

Query: 781 EDYKLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLT 840
           EDYKLIE+YL+ THAPTH DW LELEEVFSL+R+GE +K++ ++N L NKMLLWHGSRLT
Sbjct: 781 EDYKLIEQYLLNTHAPTHKDWSLELEEVFSLDRDGELNKYSRYKNNLHNKMLLWHGSRLT 840

Query: 841 NFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALG 900
           NFVGILSQGLRIAPPEAP TGYMFGKG+YFADLVSKSAQYCY DR NPVG M+LSEVALG
Sbjct: 841 NFVGILSQGLRIAPPEAPVTGYMFGKGLYFADLVSKSAQYCYVDRNNPVGLMLLSEVALG 900

Query: 901 EVYEVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMY 960
           ++YE+KKA  M++PPRGKHSTKG+GK VP  SE VKWR+DVVVPCGKPV S++++SELMY
Sbjct: 901 DMYELKKATSMDKPPRGKHSTKGLGKTVPLESEFVKWRDDVVVPCGKPVPSSIRSSELMY 960

Query: 961 NEYIVYNTAQVKMQFLLKVRFHHKR 981
           NEYIVYNT+QVKMQFLLKVRFHHKR
Sbjct: 961 NEYIVYNTSQVKMQFLLKVRFHHKR 980

BLAST of MC06g1531 vs. ExPASy Swiss-Prot
Match: P11103 (Poly [ADP-ribose] polymerase 1 OS=Mus musculus OX=10090 GN=Parp1 PE=1 SV=3)

HSP 1 Score: 627.9 bits (1618), Expect = 2.0e-178
Identity = 393/1049 (37.46%), Postives = 591/1049 (56.34%), Query Frame = 0

Query: 5    QKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKAKQIK 64
            ++ ++V+YAKS R+SCK C + I K++LR   MVQ+  FDG +P W H SC  K  + I+
Sbjct: 6    ERLYRVQYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGQSIR 65

Query: 65   SVD-DVEGLDSLRWEDQQKIRKYVEDSAAAAAVAD--------TPVECGIEVAQASRAAC 124
              D +V+G   LRW+DQQK++K  E    A    D        T  +   E A+++R+ C
Sbjct: 66   HPDVEVDGFSELRWDDQQKVKKTAEAGGVAGKGQDGSGGKAEKTLGDFAAEYAKSNRSMC 125

Query: 125  KLCKQKIMKGEVRLSTAL--DGKGAKGL--AWHHANCYMEQ------SPSTQVEKLAGWQ 184
            K C +KI KG++RLS  +    K   G+   W+H  C++++       P     +L G+ 
Sbjct: 126  KGCLEKIEKGQMRLSKKMVDPEKPQLGMIDRWYHPTCFVKKRDELGFRPEYSASQLKGFS 185

Query: 185  NLPASDQAAVSALIKKLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSM 244
             L A D+    AL K+LP+ +N          GKRK D     D        +V+  +S 
Sbjct: 186  LLSAEDK---EALKKQLPAIKNE---------GKRKGDEVDGTD--------EVAKKKSR 245

Query: 245  KTASDSPDSQNPSSLASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDL 304
            K      ++   S L   L+AQ + +W +KD+LKK  +  +L+E+L  N Q     E  +
Sbjct: 246  K------ETDKYSKLEKALKAQNELIWNIKDELKKACSTNDLKELLIFNQQQVPSGESAI 305

Query: 305  RDRCADGMMFGALANCPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPE 364
             DR ADGM FGAL  C  CS  L +    Y C G  +AW+KC      P R   +W  P+
Sbjct: 306  LDRVADGMAFGALLPCKECSGQLVFKSDAYYCTGDVTAWTKCMVKTQNPSR--KEWVTPK 365

Query: 365  ETGNQYLCKWFKSQKVAKPIRLLPPPSS---------SNANGNQASNSRSQSSKAESLAE 424
            E        + K  KV K  R+ PP SS         S  +   A NS + + K   L+ 
Sbjct: 366  EFRE---ISYLKKLKVKKQDRIFPPESSAPITVHWPLSVTSAPTAVNSSAPADK--PLSN 425

Query: 425  LRVAFSG-LKDSMEEWKRKIEGEGGAVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLP 484
            +++   G L  + +E K  IE  GG +     K + C+ +  +V++ N +  + ++  + 
Sbjct: 426  MKILTLGKLSQNKDEAKAVIEKLGGKLTGSANKASLCISIKKEVEKMNKKMEEVKEANIR 485

Query: 485  IVREEYLVECFRKQKKL--------------------------------PFDR----YKV 544
            +V E++L +     K L                                P  +    +K 
Sbjct: 486  VVSEDFLQDVSASTKSLQDLLSAHSLSPWGAEVKAEPGEVVAPRGKSAAPSKKSKGCFKE 545

Query: 545  ETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQ 604
            E   +S   + + +KG +AV   SGL+ + H+LE    +++ TL + D+  G NSYY LQ
Sbjct: 546  EGVNKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQ 605

Query: 605  IIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEM-SKSDAIREFKRLFLEKTGNPWEAWEQK 664
            +++DDK S   +FR WGR+G   IG  KLE+M SK +A+ +F +L+ EKTGN W +    
Sbjct: 606  LLEDDKESRYWIFRSWGRLGT-VIGSNKLEQMPSKEEAVEQFMKLYEEKTGNAWHS---- 665

Query: 665  QNFEKQPGRFFPLDIDYGVNKDMLKK--QKNYSATKLAPQLAELMKMLFNVETYRNAMME 724
            +NF K P +F+PL+IDYG +++ +KK   K  + +KL   + EL+ M+F+V++ + A++E
Sbjct: 666  KNFTKYPKKFYPLEIDYGQDEEAVKKLTVKPGTKSKLPKPVQELVGMIFDVDSMKKALVE 725

Query: 725  FEINMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIP-- 784
            +EI++ +MPLGKLSR  IQ  +  L+E+Q      +  GS  ES I+D SNRF+T+IP  
Sbjct: 726  YEIDLQKMPLGKLSRRQIQAAYSILSEVQ----QPVSQGS-SESQILDLSNRFYTLIPHD 785

Query: 785  --SIHPHIIRDEDDFKSKLKMLEALQDIEIASRLV--GFDDDNNESLDEKYKKLRCDIAP 844
                 P ++ + D  ++K++ML+ L DIE+A  L+  G DD + + +D  Y+KL+ DI  
Sbjct: 786  FGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKV 845

Query: 845  ISHESEDYKLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWH 904
            +  +SE+ ++I KY+  THA TH  + LE+ ++F +EREGE  ++ PFR +L N+ LLWH
Sbjct: 846  VDRDSEEAEVIRKYVKNTHATTHNAYDLEVIDIFKIEREGESQRYKPFR-QLHNRRLLWH 905

Query: 905  GSRLTNFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLS 964
            GSR TNF GILSQGLRIAPPEAP TGYMFGKGIYFAD+VSKSA YC+T + +P+G ++L 
Sbjct: 906  GSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLIMLG 965

Query: 965  EVALGEVYEVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKA 980
            EVALG +YE+K A ++ + P+GKHS KG+GK  P  S  +   E V VP G  + S V  
Sbjct: 966  EVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSASIT-LEGVEVPLGTGIPSGVND 1009

BLAST of MC06g1531 vs. ExPASy Swiss-Prot
Match: P09874 (Poly [ADP-ribose] polymerase 1 OS=Homo sapiens OX=9606 GN=PARP1 PE=1 SV=4)

HSP 1 Score: 626.3 bits (1614), Expect = 5.7e-178
Identity = 394/1049 (37.56%), Postives = 586/1049 (55.86%), Query Frame = 0

Query: 6    KPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKAKQIKS 65
            K ++VEYAKS R+SCK C + I K++LR   MVQ+  FDG +P W H SC  K    I+ 
Sbjct: 7    KLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRH 66

Query: 66   VD-DVEGLDSLRWEDQQKIRKYVEDSAAAAAVAD--------TPVECGIEVAQASRAACK 125
             D +V+G   LRW+DQQK++K  E         D        T  +   E A+++R+ CK
Sbjct: 67   PDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCK 126

Query: 126  LCKQKIMKGEVRLSTAL--DGKGAKGL--AWHHANCYMEQ------SPSTQVEKLAGWQN 185
             C +KI KG+VRLS  +    K   G+   W+H  C+++        P     +L G+  
Sbjct: 127  GCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSL 186

Query: 186  LPASDQAAVSALIKKLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMK 245
            L   D+    AL K+LP  ++          GKRK    GD+       V +V+  +S K
Sbjct: 187  LATEDK---EALKKQLPGVKSE---------GKRK----GDE----VDGVDEVAKKKSKK 246

Query: 246  TASDSPDSQNPSSLASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLR 305
                  +    S L   L+AQ   +W +KD+LKK  +  +L+E+L  N Q     E  + 
Sbjct: 247  ------EKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAIL 306

Query: 306  DRCADGMMFGALANCPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEE 365
            DR ADGM+FGAL  C  CS  L +    Y C G  +AW+KC      P R   +W  P+E
Sbjct: 307  DRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNR--KEWVTPKE 366

Query: 366  TGNQYLCKWFKSQKVAKPIRLLPP---------PSSSNANGNQASNSRSQSSKAESLAEL 425
                    + K  KV K  R+ PP         P  S A+   A NS + + K   L+ +
Sbjct: 367  FRE---ISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADK--PLSNM 426

Query: 426  RVAFSG-LKDSMEEWKRKIEGEGGAVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPI 485
            ++   G L  + +E K  IE  GG +     K + C+    +V++ N +  + ++  + +
Sbjct: 427  KILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRV 486

Query: 486  VREEYLVECFRKQKKL-------------------PFD------------------RYKV 545
            V E++L +     K L                   P +                  + K 
Sbjct: 487  VSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKE 546

Query: 546  ETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQ 605
            E   +S   + + +KG +AV   SGL+ + H+LE    +++ TL + D+  G NSYY LQ
Sbjct: 547  EGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQ 606

Query: 606  IIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEM-SKSDAIREFKRLFLEKTGNPWEAWEQK 665
            +++DDK +   +FR WGRVG   IG  KLE+M SK DAI  F +L+ EKTGN W +    
Sbjct: 607  LLEDDKENRYWIFRSWGRVGT-VIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHS---- 666

Query: 666  QNFEKQPGRFFPLDIDYGVNKDMLKKQKNYSAT--KLAPQLAELMKMLFNVETYRNAMME 725
            +NF K P +F+PL+IDYG +++ +KK      T  KL   + +L+KM+F+VE+ + AM+E
Sbjct: 667  KNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVE 726

Query: 726  FEINMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIP-- 785
            +EI++ +MPLGKLS+  IQ  +  L+E+Q     ++  GS  +S I+D SNRF+T+IP  
Sbjct: 727  YEIDLQKMPLGKLSKRQIQAAYSILSEVQ----QAVSQGS-SDSQILDLSNRFYTLIPHD 786

Query: 786  --SIHPHIIRDEDDFKSKLKMLEALQDIEIASRLV--GFDDDNNESLDEKYKKLRCDIAP 845
                 P ++ + D  ++K++ML+ L DIE+A  L+  G DD + + +D  Y+KL+ DI  
Sbjct: 787  FGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKV 846

Query: 846  ISHESEDYKLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWH 905
            +  +SE+ ++I KY+  THA TH  + LE+ ++F +EREGE  ++ PF+ +L N+ LLWH
Sbjct: 847  VDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFK-QLHNRRLLWH 906

Query: 906  GSRLTNFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLS 965
            GSR TNF GILSQGLRIAPPEAP TGYMFGKGIYFAD+VSKSA YC+T + +P+G ++L 
Sbjct: 907  GSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLG 966

Query: 966  EVALGEVYEVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKA 980
            EVALG +YE+K A ++ + P+GKHS KG+GK  P  S ++   + V VP G  ++S V  
Sbjct: 967  EVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANIS-LDGVDVPLGTGISSGVND 1010

BLAST of MC06g1531 vs. NCBI nr
Match: XP_022145931.1 (poly [ADP-ribose] polymerase 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1941 bits (5029), Expect = 0.0
Identity = 977/980 (99.69%), Postives = 979/980 (99.90%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA
Sbjct: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ
Sbjct: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQ+LPASDQAAVSALIK
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQSLPASDQAAVSALIK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL
Sbjct: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
           ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN
Sbjct: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK
Sbjct: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
           VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG
Sbjct: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE
Sbjct: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
           TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI
Sbjct: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDCHVFRKWGRVGNEKIGG+KLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN
Sbjct: 541 IQDDKSSDCHVFRKWGRVGNEKIGGVKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKDM KKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDMPKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           YNTAQVKMQFLLKVRFHHKR
Sbjct: 961 YNTAQVKMQFLLKVRFHHKR 980

BLAST of MC06g1531 vs. NCBI nr
Match: XP_022145930.1 (poly [ADP-ribose] polymerase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1936 bits (5016), Expect = 0.0
Identity = 977/982 (99.49%), Postives = 979/982 (99.69%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA
Sbjct: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ
Sbjct: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQ+LPASDQAAVSALIK
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQSLPASDQAAVSALIK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL
Sbjct: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
           ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN
Sbjct: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK
Sbjct: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
           VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG
Sbjct: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420

Query: 421 AVHAKIKK--DTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYK 480
           AVHAKIKK  DTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYK
Sbjct: 421 AVHAKIKKGADTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYK 480

Query: 481 VETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYIL 540
           VETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYIL
Sbjct: 481 VETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYIL 540

Query: 541 QIIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQK 600
           QIIQDDKSSDCHVFRKWGRVGNEKIGG+KLEEMSKSDAIREFKRLFLEKTGNPWEAWEQK
Sbjct: 541 QIIQDDKSSDCHVFRKWGRVGNEKIGGVKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQK 600

Query: 601 QNFEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFE 660
           QNFEKQPGRFFPLDIDYGVNKDM KKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFE
Sbjct: 601 QNFEKQPGRFFPLDIDYGVNKDMPKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFE 660

Query: 661 INMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHP 720
           INMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHP
Sbjct: 661 INMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHP 720

Query: 721 HIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDY 780
           HIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDY
Sbjct: 721 HIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDY 780

Query: 781 KLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFV 840
           KLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFV
Sbjct: 781 KLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFV 840

Query: 841 GILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVY 900
           GILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVY
Sbjct: 841 GILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVY 900

Query: 901 EVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEY 960
           EVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEY
Sbjct: 901 EVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEY 960

Query: 961 IVYNTAQVKMQFLLKVRFHHKR 980
           IVYNTAQVKMQFLLKVRFHHKR
Sbjct: 961 IVYNTAQVKMQFLLKVRFHHKR 982

BLAST of MC06g1531 vs. NCBI nr
Match: XP_038888831.1 (poly [ADP-ribose] polymerase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1758 bits (4554), Expect = 0.0
Identity = 879/980 (89.69%), Postives = 923/980 (94.18%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           M NPQKPWKVEYAKSSRSSCKTCK PI KENLRFGKMVQATQFDGFMPMWNHA+CILKKA
Sbjct: 1   MENPQKPWKVEYAKSSRSSCKTCKSPIQKENLRFGKMVQATQFDGFMPMWNHAACILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKS+DDVEGLDSLRWEDQQKIRKYVEDS AAAAVA TPVE GIEV+Q SRA+CK CKQ
Sbjct: 61  KQIKSIDDVEGLDSLRWEDQQKIRKYVEDSVAAAAVAVTPVESGIEVSQTSRASCKHCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAW+HANCYMEQSPSTQVEKLAGWQNLP SDQAAVSAL+K
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWYHANCYMEQSPSTQVEKLAGWQNLPPSDQAAVSALVK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           K  S   NE +Q  SK GKRKKD   DQDSKVTKAVGDVS SRSMK A  S D QN S L
Sbjct: 181 KPSSAVKNEEKQTTSKAGKRKKDTVEDQDSKVTKAVGDVSESRSMKNAIVSADPQNSSDL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
            SKLEAQ+K LWKLKDDLKKHVT AELREMLESN QDSTGSELDLRDRCADGMMFGALA 
Sbjct: 241 VSKLEAQSKGLWKLKDDLKKHVTTAELREMLESNDQDSTGSELDLRDRCADGMMFGALAK 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPIC+ SLRYSGGMYRCHGYQSAWSKCSYS  EPER+KGKWKVPEETGNQYL KWFKSQK
Sbjct: 301 CPICAGSLRYSGGMYRCHGYQSAWSKCSYSTCEPERLKGKWKVPEETGNQYLSKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
            AKPIRLLPPPSSS+ANGNQASNS+SQSSKAESLAELRV+F GLKDSM EWKRKIEGEGG
Sbjct: 361 GAKPIRLLPPPSSSSANGNQASNSQSQSSKAESLAELRVSFCGLKDSMGEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVHAKIKKDTNCLVVSGDVDEYN E +KAR+MK+PIVREEY+V+CFRKQKKLP+DRYKVE
Sbjct: 421 AVHAKIKKDTNCLVVSGDVDEYNTEMKKARRMKIPIVREEYVVDCFRKQKKLPYDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
            TGESTS+VTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTG+NSYYILQI
Sbjct: 481 ATGESTSLVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGINSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDC++FRKWGRVGNEKIGG+KL+EM+KSDAIREFKRLFLEKTGNPWEAWEQK N
Sbjct: 541 IQDDKSSDCYIFRKWGRVGNEKIGGVKLDEMTKSDAIREFKRLFLEKTGNPWEAWEQKLN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKDM KK KNYS TKLAPQLAELMKMLFNVETYR AMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDMPKKPKNYSTTKLAPQLAELMKMLFNVETYRAAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           M+EMPLGKLSRSNIQKGFEALTEIQNLLNSS+HD SMKESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MAEMPLGKLSRSNIQKGFEALTEIQNLLNSSMHDPSMKESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFDDD++ESLD+KYKKL CDIAP+SHESEDYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDSDESLDDKYKKLHCDIAPLSHESEDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYL+TTHAPTH DW LELEEVFSLEREGE+DK+APFR KL+NKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLLTTHAPTHTDWALELEEVFSLEREGEFDKYAPFRQKLKNKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDR NPVGFM+LSEVALGEV E+
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRNNPVGFMILSEVALGEVCEL 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYME+PPRGKHSTKG+GKK+PA SEHVKW++DVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMEKPPRGKHSTKGLGKKMPAPSEHVKWKDDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           Y+TAQVKMQFLLKVRFHHKR
Sbjct: 961 YDTAQVKMQFLLKVRFHHKR 980

BLAST of MC06g1531 vs. NCBI nr
Match: XP_022963631.1 (poly [ADP-ribose] polymerase 1 [Cucurbita moschata])

HSP 1 Score: 1757 bits (4550), Expect = 0.0
Identity = 875/980 (89.29%), Postives = 924/980 (94.29%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           M NPQKPWKVEYAKSSRSSCKTCK PI KENLRFGKMVQATQFDGFMPMWNH +CILKKA
Sbjct: 1   MENPQKPWKVEYAKSSRSSCKTCKSPIQKENLRFGKMVQATQFDGFMPMWNHGTCILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKS+DDVEGLDSLRWEDQQKIR+YVEDSAAA AVA TPVECGIEV+Q SRA+CK CKQ
Sbjct: 61  KQIKSIDDVEGLDSLRWEDQQKIRQYVEDSAAATAVAVTPVECGIEVSQTSRASCKRCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAW+HANCYMEQSPSTQV KLAGWQNLP+SDQAAVSAL+K
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWYHANCYMEQSPSTQVSKLAGWQNLPSSDQAAVSALVK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           K PS + NE +Q  SK GKRKKD   DQDSKVTKAVGDVS SRSMK A  + D QN S L
Sbjct: 181 KPPSADKNEEKQT-SKAGKRKKDTMTDQDSKVTKAVGDVSESRSMKNAIVTADPQNSSDL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
            SKLEAQ+KELWKLKDDLKK+VT  E+REMLESN QDSTGSELDLRDRCADGMMFGALA 
Sbjct: 241 VSKLEAQSKELWKLKDDLKKNVTTVEMREMLESNDQDSTGSELDLRDRCADGMMFGALAK 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPICS S+RYSGGMYRCHGYQSAWSKCSYS HEPER+KGKWKVPEET NQYL KWFKSQK
Sbjct: 301 CPICSGSIRYSGGMYRCHGYQSAWSKCSYSTHEPERLKGKWKVPEETSNQYLSKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
           VAKPIRLLPPPSS++ANGNQ SNS+SQSSKAESLAELRV+F GLKDS+ EWKRKIEGEGG
Sbjct: 361 VAKPIRLLPPPSSNSANGNQGSNSQSQSSKAESLAELRVSFCGLKDSVGEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVH KIKKDTNCLVVSGD+DEYN E +KAR+ K+PIVREEYLV+CF+KQKKLP+DRYKVE
Sbjct: 421 AVHPKIKKDTNCLVVSGDMDEYNAEMKKARRTKVPIVREEYLVDCFKKQKKLPYDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
             GESTS+VTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTG+NSYYILQI
Sbjct: 481 AAGESTSLVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGINSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDC+VFRKWGRVGNEKIGG+KLEEMSK DAIREFKRLFLEKTGNPWEAWEQKQN
Sbjct: 541 IQDDKSSDCYVFRKWGRVGNEKIGGVKLEEMSKPDAIREFKRLFLEKTGNPWEAWEQKQN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKD+ KK KNYSATKLAPQLAELMKMLFNVETYR AMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDITKKPKNYSATKLAPQLAELMKMLFNVETYRAAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           M+EMPLGKLSR NIQKGFEALTEIQNLLNSS+HD SM+ESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MAEMPLGKLSRRNIQKGFEALTEIQNLLNSSMHDPSMRESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFDD+++ESLD+KYKKLRCDIAPISHESEDYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDNSDESLDDKYKKLRCDIAPISHESEDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYL+TTHAPTH DW LELEEVFSLEREGE+DKFAPFR KLENKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLLTTHAPTHTDWALELEEVFSLEREGEFDKFAPFRQKLENKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTD+ NPVGFM+LSEVALGEVYE+
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDKNNPVGFMLLSEVALGEVYEL 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYMERPPRGKHSTKG+GKKVPA SEHVKW+EDVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMERPPRGKHSTKGLGKKVPAASEHVKWKEDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           Y+TAQVK+QFLLKVRFHHKR
Sbjct: 961 YDTAQVKLQFLLKVRFHHKR 979

BLAST of MC06g1531 vs. NCBI nr
Match: XP_038888832.1 (poly [ADP-ribose] polymerase 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1754 bits (4543), Expect = 0.0
Identity = 879/980 (89.69%), Postives = 923/980 (94.18%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           M NPQKPWKVEYAKSSRSSCKTCK PI KENLRFGKMVQATQFDGFMPMWNHA+CILKKA
Sbjct: 1   MENPQKPWKVEYAKSSRSSCKTCKSPIQKENLRFGKMVQATQFDGFMPMWNHAACILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKS+DDVEGLDSLRWEDQQKIRKYVEDS AAAAVA TPVE GIEV+Q SRA+CK CKQ
Sbjct: 61  KQIKSIDDVEGLDSLRWEDQQKIRKYVEDSVAAAAVAVTPVESGIEVSQTSRASCKHCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAW+HANCYMEQSPSTQVEKLAGWQNLP SDQAAVSAL+K
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWYHANCYMEQSPSTQVEKLAGWQNLPPSDQAAVSALVK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           K  S   NE +Q  SK GKRKKD   DQDSKVTKAVGDVS SRSMK A  S D QN S L
Sbjct: 181 KPSSAVKNE-KQTTSKAGKRKKDTVEDQDSKVTKAVGDVSESRSMKNAIVSADPQNSSDL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
            SKLEAQ+K LWKLKDDLKKHVT AELREMLESN QDSTGSELDLRDRCADGMMFGALA 
Sbjct: 241 VSKLEAQSKGLWKLKDDLKKHVTTAELREMLESNDQDSTGSELDLRDRCADGMMFGALAK 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPIC+ SLRYSGGMYRCHGYQSAWSKCSYS  EPER+KGKWKVPEETGNQYL KWFKSQK
Sbjct: 301 CPICAGSLRYSGGMYRCHGYQSAWSKCSYSTCEPERLKGKWKVPEETGNQYLSKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
            AKPIRLLPPPSSS+ANGNQASNS+SQSSKAESLAELRV+F GLKDSM EWKRKIEGEGG
Sbjct: 361 GAKPIRLLPPPSSSSANGNQASNSQSQSSKAESLAELRVSFCGLKDSMGEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVHAKIKKDTNCLVVSGDVDEYN E +KAR+MK+PIVREEY+V+CFRKQKKLP+DRYKVE
Sbjct: 421 AVHAKIKKDTNCLVVSGDVDEYNTEMKKARRMKIPIVREEYVVDCFRKQKKLPYDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
            TGESTS+VTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTG+NSYYILQI
Sbjct: 481 ATGESTSLVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGINSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDC++FRKWGRVGNEKIGG+KL+EM+KSDAIREFKRLFLEKTGNPWEAWEQK N
Sbjct: 541 IQDDKSSDCYIFRKWGRVGNEKIGGVKLDEMTKSDAIREFKRLFLEKTGNPWEAWEQKLN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKDM KK KNYS TKLAPQLAELMKMLFNVETYR AMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDMPKKPKNYSTTKLAPQLAELMKMLFNVETYRAAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           M+EMPLGKLSRSNIQKGFEALTEIQNLLNSS+HD SMKESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MAEMPLGKLSRSNIQKGFEALTEIQNLLNSSMHDPSMKESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFDDD++ESLD+KYKKL CDIAP+SHESEDYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDSDESLDDKYKKLHCDIAPLSHESEDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYL+TTHAPTH DW LELEEVFSLEREGE+DK+APFR KL+NKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLLTTHAPTHTDWALELEEVFSLEREGEFDKYAPFRQKLKNKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDR NPVGFM+LSEVALGEV E+
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRNNPVGFMILSEVALGEVCEL 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYME+PPRGKHSTKG+GKK+PA SEHVKW++DVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMEKPPRGKHSTKGLGKKMPAPSEHVKWKDDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           Y+TAQVKMQFLLKVRFHHKR
Sbjct: 961 YDTAQVKMQFLLKVRFHHKR 979

BLAST of MC06g1531 vs. ExPASy TrEMBL
Match: A0A6J1CXV4 (Poly [ADP-ribose] polymerase OS=Momordica charantia OX=3673 GN=LOC111015277 PE=4 SV=1)

HSP 1 Score: 1941 bits (5029), Expect = 0.0
Identity = 977/980 (99.69%), Postives = 979/980 (99.90%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA
Sbjct: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ
Sbjct: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQ+LPASDQAAVSALIK
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQSLPASDQAAVSALIK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL
Sbjct: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
           ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN
Sbjct: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK
Sbjct: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
           VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG
Sbjct: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE
Sbjct: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
           TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI
Sbjct: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDCHVFRKWGRVGNEKIGG+KLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN
Sbjct: 541 IQDDKSSDCHVFRKWGRVGNEKIGGVKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKDM KKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDMPKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           YNTAQVKMQFLLKVRFHHKR
Sbjct: 961 YNTAQVKMQFLLKVRFHHKR 980

BLAST of MC06g1531 vs. ExPASy TrEMBL
Match: A0A6J1CVV4 (Poly [ADP-ribose] polymerase OS=Momordica charantia OX=3673 GN=LOC111015277 PE=4 SV=1)

HSP 1 Score: 1936 bits (5016), Expect = 0.0
Identity = 977/982 (99.49%), Postives = 979/982 (99.69%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA
Sbjct: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ
Sbjct: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQ+LPASDQAAVSALIK
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQSLPASDQAAVSALIK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL
Sbjct: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
           ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN
Sbjct: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK
Sbjct: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
           VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG
Sbjct: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420

Query: 421 AVHAKIKK--DTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYK 480
           AVHAKIKK  DTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYK
Sbjct: 421 AVHAKIKKGADTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYK 480

Query: 481 VETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYIL 540
           VETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYIL
Sbjct: 481 VETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYIL 540

Query: 541 QIIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQK 600
           QIIQDDKSSDCHVFRKWGRVGNEKIGG+KLEEMSKSDAIREFKRLFLEKTGNPWEAWEQK
Sbjct: 541 QIIQDDKSSDCHVFRKWGRVGNEKIGGVKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQK 600

Query: 601 QNFEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFE 660
           QNFEKQPGRFFPLDIDYGVNKDM KKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFE
Sbjct: 601 QNFEKQPGRFFPLDIDYGVNKDMPKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFE 660

Query: 661 INMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHP 720
           INMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHP
Sbjct: 661 INMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHP 720

Query: 721 HIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDY 780
           HIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDY
Sbjct: 721 HIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDY 780

Query: 781 KLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFV 840
           KLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFV
Sbjct: 781 KLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFV 840

Query: 841 GILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVY 900
           GILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVY
Sbjct: 841 GILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVY 900

Query: 901 EVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEY 960
           EVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEY
Sbjct: 901 EVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEY 960

Query: 961 IVYNTAQVKMQFLLKVRFHHKR 980
           IVYNTAQVKMQFLLKVRFHHKR
Sbjct: 961 IVYNTAQVKMQFLLKVRFHHKR 982

BLAST of MC06g1531 vs. ExPASy TrEMBL
Match: A0A6J1HGL0 (Poly [ADP-ribose] polymerase OS=Cucurbita moschata OX=3662 GN=LOC111463901 PE=4 SV=1)

HSP 1 Score: 1757 bits (4550), Expect = 0.0
Identity = 875/980 (89.29%), Postives = 924/980 (94.29%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           M NPQKPWKVEYAKSSRSSCKTCK PI KENLRFGKMVQATQFDGFMPMWNH +CILKKA
Sbjct: 1   MENPQKPWKVEYAKSSRSSCKTCKSPIQKENLRFGKMVQATQFDGFMPMWNHGTCILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKS+DDVEGLDSLRWEDQQKIR+YVEDSAAA AVA TPVECGIEV+Q SRA+CK CKQ
Sbjct: 61  KQIKSIDDVEGLDSLRWEDQQKIRQYVEDSAAATAVAVTPVECGIEVSQTSRASCKRCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAW+HANCYMEQSPSTQV KLAGWQNLP+SDQAAVSAL+K
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWYHANCYMEQSPSTQVSKLAGWQNLPSSDQAAVSALVK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           K PS + NE +Q  SK GKRKKD   DQDSKVTKAVGDVS SRSMK A  + D QN S L
Sbjct: 181 KPPSADKNEEKQT-SKAGKRKKDTMTDQDSKVTKAVGDVSESRSMKNAIVTADPQNSSDL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
            SKLEAQ+KELWKLKDDLKK+VT  E+REMLESN QDSTGSELDLRDRCADGMMFGALA 
Sbjct: 241 VSKLEAQSKELWKLKDDLKKNVTTVEMREMLESNDQDSTGSELDLRDRCADGMMFGALAK 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPICS S+RYSGGMYRCHGYQSAWSKCSYS HEPER+KGKWKVPEET NQYL KWFKSQK
Sbjct: 301 CPICSGSIRYSGGMYRCHGYQSAWSKCSYSTHEPERLKGKWKVPEETSNQYLSKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
           VAKPIRLLPPPSS++ANGNQ SNS+SQSSKAESLAELRV+F GLKDS+ EWKRKIEGEGG
Sbjct: 361 VAKPIRLLPPPSSNSANGNQGSNSQSQSSKAESLAELRVSFCGLKDSVGEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVH KIKKDTNCLVVSGD+DEYN E +KAR+ K+PIVREEYLV+CF+KQKKLP+DRYKVE
Sbjct: 421 AVHPKIKKDTNCLVVSGDMDEYNAEMKKARRTKVPIVREEYLVDCFKKQKKLPYDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
             GESTS+VTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTG+NSYYILQI
Sbjct: 481 AAGESTSLVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGINSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDC+VFRKWGRVGNEKIGG+KLEEMSK DAIREFKRLFLEKTGNPWEAWEQKQN
Sbjct: 541 IQDDKSSDCYVFRKWGRVGNEKIGGVKLEEMSKPDAIREFKRLFLEKTGNPWEAWEQKQN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKD+ KK KNYSATKLAPQLAELMKMLFNVETYR AMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDITKKPKNYSATKLAPQLAELMKMLFNVETYRAAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           M+EMPLGKLSR NIQKGFEALTEIQNLLNSS+HD SM+ESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MAEMPLGKLSRRNIQKGFEALTEIQNLLNSSMHDPSMRESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFDD+++ESLD+KYKKLRCDIAPISHESEDYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDNSDESLDDKYKKLRCDIAPISHESEDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYL+TTHAPTH DW LELEEVFSLEREGE+DKFAPFR KLENKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLLTTHAPTHTDWALELEEVFSLEREGEFDKFAPFRQKLENKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTD+ NPVGFM+LSEVALGEVYE+
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDKNNPVGFMLLSEVALGEVYEL 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYMERPPRGKHSTKG+GKKVPA SEHVKW+EDVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMERPPRGKHSTKGLGKKVPAASEHVKWKEDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           Y+TAQVK+QFLLKVRFHHKR
Sbjct: 961 YDTAQVKLQFLLKVRFHHKR 979

BLAST of MC06g1531 vs. ExPASy TrEMBL
Match: A0A6J1HUD8 (Poly [ADP-ribose] polymerase OS=Cucurbita maxima OX=3661 GN=LOC111466943 PE=4 SV=1)

HSP 1 Score: 1744 bits (4516), Expect = 0.0
Identity = 868/980 (88.57%), Postives = 915/980 (93.37%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           M NPQKPWKVEYAKSSRSSCKTCK PI KENLRFGKMVQATQFDGFMPMWNH +CILKKA
Sbjct: 1   MENPQKPWKVEYAKSSRSSCKTCKSPIQKENLRFGKMVQATQFDGFMPMWNHGTCILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKS+DDVEGLDSLRWEDQQKIR+YVEDSAAA AVA TPVECGIEV+Q SRA+CK CKQ
Sbjct: 61  KQIKSIDDVEGLDSLRWEDQQKIRQYVEDSAAATAVAVTPVECGIEVSQTSRASCKRCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLSTALDGKGAKGLAW+HANCYMEQSPS QV KLAGWQNLP+SDQAAVSAL+K
Sbjct: 121 KIMKGEVRLSTALDGKGAKGLAWYHANCYMEQSPSAQVSKLAGWQNLPSSDQAAVSALVK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           K PS + NE +QA SK GKRKKD   DQDSKVTKAVGDVS SRSMK A  + D QN S L
Sbjct: 181 KPPSADKNEAKQATSKAGKRKKDTMTDQDSKVTKAVGDVSESRSMKNAIVTADPQNSSDL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
            SKLEAQ+KELWKLKDDLKK+VT  E+REMLESN QDSTGSELDLRDRCADGMMFGALA 
Sbjct: 241 VSKLEAQSKELWKLKDDLKKNVTTVEMREMLESNDQDSTGSELDLRDRCADGMMFGALAK 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPICS S+RYSGGMYRCHGYQSAWSKCSYS  EPER+ GKWKVPEET NQYL KWFKSQK
Sbjct: 301 CPICSGSIRYSGGMYRCHGYQSAWSKCSYSTREPERLTGKWKVPEETSNQYLSKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
            AKPIRLLPPPS S+ANGNQ  NS+SQSSKAESLAELRV+F GL DS+ EWKRKIEGEGG
Sbjct: 361 GAKPIRLLPPPSFSSANGNQGPNSQSQSSKAESLAELRVSFCGLTDSVGEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVH KIKKDTNCLVVSGD+DEYN E +KAR+ K+PIVREEYLV+CFRKQKKLP+DRYKVE
Sbjct: 421 AVHPKIKKDTNCLVVSGDMDEYNAEMKKARRTKIPIVREEYLVDCFRKQKKLPYDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
             GESTS+VTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTG+NSYYILQI
Sbjct: 481 AAGESTSLVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGINSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDC+VFRKWGRVGNEKIGG+KLEEM K DAIREFKRLFLEKTGNPWEAWEQKQN
Sbjct: 541 IQDDKSSDCYVFRKWGRVGNEKIGGVKLEEMQKPDAIREFKRLFLEKTGNPWEAWEQKQN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKDM KK KNYS TKLAPQLAELMKMLFNVETYR AMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDMTKKPKNYSVTKLAPQLAELMKMLFNVETYRAAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           M+EMPLGKLSR NIQKGFEALTEIQNLLNSS+HD SMKESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MAEMPLGKLSRRNIQKGFEALTEIQNLLNSSVHDPSMKESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFDD+++ESLD+KYKKL CDIAPISHES DYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDNSDESLDDKYKKLHCDIAPISHESVDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYL+TTHAPTH DW LELEEVFSLEREGE+DKFAPFR+KL+NKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLLTTHAPTHTDWALELEEVFSLEREGEFDKFAPFRHKLKNKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTD+ NPVGFM+LSEVALGEVYE+
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDKNNPVGFMLLSEVALGEVYEL 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYMERPPRGKHSTKG+GKKVPA SEHVKW+EDVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMERPPRGKHSTKGLGKKVPAASEHVKWKEDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           Y+TAQVK+QFLLKVRFHHKR
Sbjct: 961 YDTAQVKLQFLLKVRFHHKR 980

BLAST of MC06g1531 vs. ExPASy TrEMBL
Match: A0A0A0L7J5 (Poly [ADP-ribose] polymerase OS=Cucumis sativus OX=3659 GN=Csa_3G398930 PE=4 SV=1)

HSP 1 Score: 1709 bits (4426), Expect = 0.0
Identity = 855/980 (87.24%), Postives = 899/980 (91.73%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MA PQKPWKVEYAKSSRSSCKTCK PI KENLRFGKMVQATQFDGFMPMWNHA+CILKKA
Sbjct: 1   MAEPQKPWKVEYAKSSRSSCKTCKSPIQKENLRFGKMVQATQFDGFMPMWNHAACILKKA 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADTPVECGIEVAQASRAACKLCKQ 120
           KQIKS+DDVEGLDSLRWEDQ KIR+YVEDS AAAAV  TP+E GIEV+Q SRA+CK CKQ
Sbjct: 61  KQIKSIDDVEGLDSLRWEDQLKIRQYVEDSVAAAAVVVTPIEYGIEVSQTSRASCKHCKQ 120

Query: 121 KIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPASDQAAVSALIK 180
           KIMKGEVRLST LDGKG KGLAW+HANCYMEQ PS QVEKLAGWQNLP SDQAA+S L+K
Sbjct: 121 KIMKGEVRLSTVLDGKGTKGLAWYHANCYMEQCPSAQVEKLAGWQNLPPSDQAAISTLVK 180

Query: 181 KLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSL 240
           K  S   NE +Q  SK GKRKKD   DQDSKVTKA GDVS SRSMK A  S DSQN + L
Sbjct: 181 KPSSAVKNEEKQTTSKAGKRKKDTAEDQDSKVTKATGDVSESRSMKNAIVSADSQNSADL 240

Query: 241 ASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDLRDRCADGMMFGALAN 300
            SKLEAQ+K LWKLKDDLKKHVT +ELREMLESN QDSTGSELDLRDRCADGMMFGALA 
Sbjct: 241 VSKLEAQSKGLWKLKDDLKKHVTTSELREMLESNDQDSTGSELDLRDRCADGMMFGALAK 300

Query: 301 CPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPEETGNQYLCKWFKSQK 360
           CPIC  SL YS GMYRCHGYQSAWSKCSYS  EPER++GKWKVPEETGN YL KWFKSQK
Sbjct: 301 CPICFGSLCYSRGMYRCHGYQSAWSKCSYSTCEPERLRGKWKVPEETGNLYLSKWFKSQK 360

Query: 361 VAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLKDSMEEWKRKIEGEGG 420
            AKPIRLLPPP+SS  N NQ SN +SQSS +E+LAELRV+F GLKDSM EWKRKIEGEGG
Sbjct: 361 GAKPIRLLPPPTSSTTNSNQTSNGQSQSSNSENLAELRVSFYGLKDSMGEWKRKIEGEGG 420

Query: 421 AVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVECFRKQKKLPFDRYKVE 480
           AVHAKIKKDTNCLVVSG VDEYN E +KAR+MK+PIVREEYLV+CFRKQKKLP+DRYKVE
Sbjct: 421 AVHAKIKKDTNCLVVSGYVDEYNPEMKKARRMKIPIVREEYLVDCFRKQKKLPYDRYKVE 480

Query: 481 TTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLSTGVNSYYILQI 540
            T ESTS+VTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDL TG+NSYYILQI
Sbjct: 481 ATSESTSLVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNMSDLLTGINSYYILQI 540

Query: 541 IQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPWEAWEQKQN 600
           IQDDKSSDC+VFRKWGRVGNEKIGG+KLEEM+KSDAIREFKRLFLEKTGNPWEAWEQK N
Sbjct: 541 IQDDKSSDCYVFRKWGRVGNEKIGGVKLEEMTKSDAIREFKRLFLEKTGNPWEAWEQKLN 600

Query: 601 FEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLFNVETYRNAMMEFEIN 660
           FEKQPGRFFPLDIDYGVNKDM KK KNY ATKLAPQLAELMKMLFNVETYR AMMEFEIN
Sbjct: 601 FEKQPGRFFPLDIDYGVNKDMPKKPKNYPATKLAPQLAELMKMLFNVETYRAAMMEFEIN 660

Query: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHI 720
           MSEMPLGKLSRSNIQKGFEALTEIQNLLNSS+HD  MKESLIIDASNRFFTVIPSIHPHI
Sbjct: 661 MSEMPLGKLSRSNIQKGFEALTEIQNLLNSSVHDPYMKESLIIDASNRFFTVIPSIHPHI 720

Query: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLRCDIAPISHESEDYKL 780
           IRDEDDFKSKLKMLEALQDIEIASRLVGFD D++ESLD+KYKKL CDIAPISHESEDYKL
Sbjct: 721 IRDEDDFKSKLKMLEALQDIEIASRLVGFDGDSHESLDDKYKKLHCDIAPISHESEDYKL 780

Query: 781 IEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENKMLLWHGSRLTNFVGI 840
           IEKYL+ THAPTH DW LELEEVFSLEREGE+DKF PFR KL+NKMLLWHGSRLTNFVGI
Sbjct: 781 IEKYLLNTHAPTHTDWALELEEVFSLEREGEFDKFVPFRQKLKNKMLLWHGSRLTNFVGI 840

Query: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVALGEVYEV 900
           LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDR NP+GFM+LSEVALGEVYE+
Sbjct: 841 LSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRNNPIGFMILSEVALGEVYEL 900

Query: 901 KKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASNVKASELMYNEYIV 960
           KKAEYME+PPRGKHSTKG+GKKVPA  EHVKW+EDVVVPCGKPVASNVKASELMYNEYIV
Sbjct: 901 KKAEYMEKPPRGKHSTKGLGKKVPAALEHVKWKEDVVVPCGKPVASNVKASELMYNEYIV 960

Query: 961 YNTAQVKMQFLLKVRFHHKR 980
           Y+TAQVKMQFLLKVRFHHKR
Sbjct: 961 YDTAQVKMQFLLKVRFHHKR 980

BLAST of MC06g1531 vs. TAIR 10
Match: AT2G31320.1 (poly(ADP-ribose) polymerase 2 )

HSP 1 Score: 1357.8 bits (3513), Expect = 0.0e+00
Identity = 683/995 (68.64%), Postives = 807/995 (81.11%), Query Frame = 0

Query: 1   MANPQKPWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKA 60
           MA+P KPW+ EYAKSSRSSCKTCK  I+KEN R GK+VQ+T FDG MPMWNHASCILKK 
Sbjct: 1   MASPHKPWRAEYAKSSRSSCKTCKSVINKENFRLGKLVQSTHFDGIMPMWNHASCILKKT 60

Query: 61  KQIKSVDDVEGLDSLRWEDQQKIRKYVEDSAAAAAVADT-----------PVECGIEVAQ 120
           KQIKSVDDVEG++SLRWEDQQKIRKYVE  A +     T            +E GIEV+Q
Sbjct: 61  KQIKSVDDVEGIESLRWEDQQKIRKYVESGAGSNTSTSTGTSTSSTANNAKLEYGIEVSQ 120

Query: 121 ASRAACKLCKQKIMKGEVRLSTALDGKGAKGLAWHHANCYMEQSPSTQVEKLAGWQNLPA 180
            SRA C+ C +KI+KGEVR+ +  +G G KGL WHHA C++E S ST++E L+GW+++P 
Sbjct: 121 TSRAGCRKCSEKILKGEVRIFSKPEGPGNKGLMWHHAKCFLEMSSSTELESLSGWRSIPD 180

Query: 181 SDQAAVSALIKK-LPS--TENNEPRQAASKVG-KRKKDATGDQDSKVTKAVGDVSVSRSM 240
           SDQ A+  L+KK LP+  TE  E RQ  S+ G KRK D+  ++ SK+ K+  D+S S ++
Sbjct: 181 SDQEALLPLVKKALPAAKTETAEARQTNSRAGTKRKNDSVDNEKSKLAKSSFDMSTSGAL 240

Query: 241 KTASDSPDSQNPSSLASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGSELDL 300
           +          P S   ++EAQTKELW LKDDLKK+VT+AELREMLE N Q + GSELDL
Sbjct: 241 Q----------PCSKEKEMEAQTKELWDLKDDLKKYVTSAELREMLEVNEQSTRGSELDL 300

Query: 301 RDRCADGMMFGALANCPICSASLRYSGGMYRCHGYQSAWSKCSYSAHEPERIKGKWKVPE 360
           RD+CADGMMFG LA CP+CS  L +SGG+YRCHGY S WSKCS+S  +P+RIKGKWK+P+
Sbjct: 301 RDKCADGMMFGPLALCPMCSGHLSFSGGLYRCHGYISEWSKCSHSTLDPDRIKGKWKIPD 360

Query: 361 ETGNQYLCKWFKSQKVAKPIRLLPPPSSSNANGNQASNSRSQSSKAESLAELRVAFSGLK 420
           ET NQ+L KW KSQK  KP R+L P  S   +  Q S   + SS++E LA+L+V+ +G  
Sbjct: 361 ETENQFLLKWNKSQKSVKPKRILRPVLSGETSQGQGSKDATDSSRSERLADLKVSIAGNT 420

Query: 421 DSMEEWKRKIEGEGGAVHAKIKKDTNCLVVSGDVDEYNLETRKARKMKLPIVREEYLVEC 480
              + WK++IE  G   HA +KK T+CLVV G  D  + E RKAR+MK+ IVRE+YLV+C
Sbjct: 421 KERQPWKKRIEEAGAEFHANVKKGTSCLVVCGLTDIRDAEMRKARRMKVAIVREDYLVDC 480

Query: 481 FRKQKKLPFDRYKVETTGESTSMVTVKVKGRSAVHESSGLQDTGHILEDKKSIYNTTLNM 540
           F+KQ+KLPFD+YK+E T E  S+VTVKVKGRSAVHE+SGLQ+  HILED  SIYNTTL+M
Sbjct: 481 FKKQRKLPFDKYKIEDTSE--SLVTVKVKGRSAVHEASGLQEHCHILEDGNSIYNTTLSM 540

Query: 541 SDLSTGVNSYYILQIIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFL 600
           SDLSTG+NSYYILQIIQ+DK SDC+VFRKWGRVGNEKIGG K+EEMSKSDA+ EFKRLFL
Sbjct: 541 SDLSTGINSYYILQIIQEDKGSDCYVFRKWGRVGNEKIGGNKVEEMSKSDAVHEFKRLFL 600

Query: 601 EKTGNPWEAWEQKQNFEKQPGRFFPLDIDYGVNKDMLKKQKNYSATKLAPQLAELMKMLF 660
           EKTGN WE+WEQK NF+KQPG+F PLDIDYGVNK + KK+   +++ LAP L ELMKMLF
Sbjct: 601 EKTGNTWESWEQKTNFQKQPGKFLPLDIDYGVNKQVAKKEPFQTSSNLAPSLIELMKMLF 660

Query: 661 NVETYRNAMMEFEINMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDA 720
           +VETYR+AMMEFEINMSEMPLGKLS+ NIQKGFEALTEIQ LL  S    +MKESL++DA
Sbjct: 661 DVETYRSAMMEFEINMSEMPLGKLSKHNIQKGFEALTEIQRLLTESDPQPTMKESLLVDA 720

Query: 721 SNRFFTVIPSIHPHIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDDNNESLDEKYKKLR 780
           SNRFFT+IPSIHPHIIRDEDDFKSK+KMLEALQDIEIASR+VGFD D+ ESLD+KYKKL 
Sbjct: 721 SNRFFTMIPSIHPHIIRDEDDFKSKVKMLEALQDIEIASRIVGFDVDSTESLDDKYKKLH 780

Query: 781 CDIAPISHESEDYKLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFAPFRNKLENK 840
           CDI+P+ H+SEDY+LIEKYL TTHAPTH +W LELEEVF+LEREGE+DK+AP R KL NK
Sbjct: 781 CDISPLPHDSEDYRLIEKYLNTTHAPTHTEWSLELEEVFALEREGEFDKYAPHREKLGNK 840

Query: 841 MLLWHGSRLTNFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVG 900
           MLLWHGSRLTNFVGIL+QGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYT +KNPVG
Sbjct: 841 MLLWHGSRLTNFVGILNQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTCKKNPVG 900

Query: 901 FMVLSEVALGEVYEVKKAEYMERPPRGKHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVA 960
            M+LSEVALGE++E+ KA+YM++PPRGKHSTKG+GKKVP  SE  KWR DV VPCGKPV+
Sbjct: 901 LMLLSEVALGEIHELTKAKYMDKPPRGKHSTKGLGKKVPQDSEFAKWRGDVTVPCGKPVS 960

Query: 961 SNVKASELMYNEYIVYNTAQVKMQFLLKVRFHHKR 981
           S VKASELMYNEYIVY+TAQVK+QFLLKVRF HKR
Sbjct: 961 SKVKASELMYNEYIVYDTAQVKLQFLLKVRFKHKR 983

BLAST of MC06g1531 vs. TAIR 10
Match: AT4G02390.1 (poly(ADP-ribose) polymerase )

HSP 1 Score: 332.8 bits (852), Expect = 9.3e-91
Identity = 191/524 (36.45%), Postives = 298/524 (56.87%), Query Frame = 0

Query: 475 DRYKVETTGESTSMVTVKVKGRSAVHE--SSGLQDTGHILEDKKSIYNTTLNMSDLSTGV 534
           D    E   +   +VT   KG + + +     ++   H+L+    +Y+  LN +++    
Sbjct: 123 DNNGFEEEKKEEKIVTATKKGAAVLDQWIPDEIKSQYHVLQRGDDVYDAILNQTNVRDNN 182

Query: 535 NSYYILQIIQDDKSSDCHVFRKWGRVGNEKIGGIKLEEMSKSDAIREFKRLFLEKTGNPW 594
           N +++LQ+++ D      V+ +WGRVG +    +     S   AI  F   F +KT N  
Sbjct: 183 NKFFVLQVLESDSKKTYMVYTRWGRVGVKGQSKLDGPYDSWDRAIEIFTNKFNDKTKN-- 242

Query: 595 EAWEQKQNFEKQPGRFFPLDIDYG-------VNKDMLKKQKNY--SATKLAPQLAELMKM 654
             W  ++ F   P  +  L++DYG       VN D+           +KL  ++A+ + +
Sbjct: 243 -YWSDRKEFIPHPKSYTWLEMDYGKEENDSPVNNDIPSSSSEVKPEQSKLDTRVAKFISL 302

Query: 655 LFNVETYRNAMMEFEINMSEMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLII 714
           + NV      MME   N +++PLGK+S+S I KG+E L  I  +++   +D +  E L  
Sbjct: 303 ICNVSMMAQHMMEIGYNANKLPLGKISKSTISKGYEVLKRISEVIDR--YDRTRLEEL-- 362

Query: 715 DASNRFFTVIP------SIHPHIIRDEDDFKSKLKMLEALQDIEIASRLVGFDDD-NNES 774
             S  F+TVIP       +   +I      K K++M+EAL +IE+A++L+  D    ++ 
Sbjct: 363 --SGEFYTVIPHDFGFKKMSQFVIDTPQKLKQKIEMVEALGEIELATKLLSVDPGLQDDP 422

Query: 775 LDEKYKKLRCDIAPISHESEDYKLIEKYLVTTHAPTHMDWGLELEEVFSLEREGEYDKFA 834
           L   Y++L C + P+ ++SE++ ++  Y+  THA TH  + +E+ ++F   R  E D+F 
Sbjct: 423 LYYHYQQLNCGLTPVGNDSEEFSMVANYMENTHAKTHSGYTVEIAQLFRASRAVEADRFQ 482

Query: 835 PFRNKLENKMLLWHGSRLTNFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYC 894
            F +  +N+MLLWHGSRLTN+ GILSQGLRIAPPEAP TGYMFGKG+YFAD+ SKSA YC
Sbjct: 483 QFSSS-KNRMLLWHGSRLTNWAGILSQGLRIAPPEAPVTGYMFGKGVYFADMFSKSANYC 542

Query: 895 YTDRKNPVGFMVLSEVALGEVYEVKKAEY-MERPPRGKHSTKGMGKKVPAVSEHVKWRED 954
           Y +     G ++L EVALG++ E+  ++Y  +  P GK STKG+GK  P  SE     + 
Sbjct: 543 YANTGANDGVLLLCEVALGDMNELLYSDYNADNLPPGKLSTKGVGKTAPNPSEAQTLEDG 602

Query: 955 VVVPCGKPVASNVKASELMYNEYIVYNTAQVKMQFLLKVRFHHK 980
           VVVP GKPV  +     L+YNEYIVYN  Q+KM+++++V+F++K
Sbjct: 603 VVVPLGKPVERSCSKGMLLYNEYIVYNVEQIKMRYVIQVKFNYK 636

BLAST of MC06g1531 vs. TAIR 10
Match: AT5G22470.1 (NAD+ ADP-ribosyltransferases;NAD+ ADP-ribosyltransferases )

HSP 1 Score: 327.8 bits (839), Expect = 3.0e-89
Identity = 248/812 (30.54%), Postives = 407/812 (50.12%), Query Frame = 0

Query: 214 KAVGDVSVS-RSMKTASDSPDSQNPSSLASKLEAQTKELWKLKDDLKKHVTNAELREMLE 273
           KA G +  S +S K A    D     + A    ++  E  K    ++++++  +++E+LE
Sbjct: 25  KAEGKLPESEQSQKKAKPENDDGRSVNGAGDAASEYNEFCKA---VEENLSIDQIKEVLE 84

Query: 274 SNGQDSTGSELDLRDRCADGMMFGALANCPICSASLRYSG-GMYRCHGYQSAWSKCSYSA 333
            NGQD +  E  L  +C D + +GALA CP+C  +L       + C G  S W  C +S 
Sbjct: 85  INGQDCSAPEETLLAQCQDLLFYGALAKCPLCGGTLICDNEKRFVCGGEISEWCSCVFST 144

Query: 334 HEPERIKGKWKVPEETGNQYLCKWFKSQKVAKPIRLLPPPSSSNANGNQASNSRSQSSKA 393
            +P R +   K+P+   N  +    K  +  K                 +   R   S  
Sbjct: 145 KDPPRKEEPVKIPDSVMNSAISDLIKKHQDPK-----------------SRPKRELGSAD 204

Query: 394 ESLAELRVAFSG-LKDSMEEWKRKIEGEGGAVHAKIKKDTNCLVVSGDVDEYNLETRKAR 453
           +    + ++  G L  + + WK+KIE  GG V   ++  T CLVVS    E    ++   
Sbjct: 205 KPFVGMMISLMGRLTRTHQYWKKKIERNGGKVSNTVQGVT-CLVVSPAERERGGTSKMVE 264

Query: 454 KMK--LPIVREEYLVECFRKQKKLPFDRYKV-------------------ETTGESTSMV 513
            M+  LP+V E +L++   K +  P + Y V                   E   ES S  
Sbjct: 265 AMEQGLPVVSEAWLIDSVEKHEAQPLEAYDVVSDLSVEGKGIPWDKQDPSEEAIESFS-A 324

Query: 514 TVKVKGRSAVHESSGLQDT-GHILEDKKSIYNTTLNMSDLSTGVNSYYILQIIQDDKSSD 573
            +K+ G+  V+  + LQ+  G I E    +YN   ++ DL  G N Y I+Q++     S+
Sbjct: 325 ELKMYGKRGVYMDTKLQERGGKIFEKDGLLYNCAFSICDLGKGRNEYCIMQLV-TVPDSN 384

Query: 574 CHVFRKWGRVGNEKIGGIKLEEMSKSD-AIREFKRLFLEKTGNPWEAWEQKQNFEKQPGR 633
            +++ K G+VG++     +LEE    + AI+EF RLF E  GN +E WE+++  +K+P +
Sbjct: 385 LNMYFKRGKVGDDPNAEERLEEWEDEEAAIKEFARLFEEIAGNEFEPWEREKKIQKKPHK 444

Query: 634 FFPLDIDYGVNKDM----LKKQKNYSA-TKLAPQLAELMKMLFNVETYRNAMMEFEINMS 693
           FFP+D+D G+        L++    SA  KL   +A  +K+L   E Y  A+ME  ++  
Sbjct: 445 FFPIDMDDGIEVRSGALGLRQLGIASAHCKLDSFVANFIKVLCGQEIYNYALMELGLDPP 504

Query: 694 EMPLGKLSRSNIQKGFEALTEIQNLLNSSIHDGSMKESLIIDASNRFFTVIPSIHPHIIR 753
           ++P+G L+  ++++  E L E    + ++   G   E++  D S+R+F+++ S  P  + 
Sbjct: 505 DLPMGMLTDIHLKRCEEVLLEFVEKVKTTKETGQKAEAMWADFSSRWFSLMHSTRPMRLH 564

Query: 754 DEDDFKS-KLKMLEALQDIEIASRLVG--FDDDNNESLDEKYKKLRCDIAPISHESEDYK 813
           D ++         E ++DI  ASRL+G    D  ++ L ++YKKL C I+ +  ESEDYK
Sbjct: 565 DVNELADHAASAFETVRDINTASRLIGDMRGDTLDDPLSDRYKKLGCKISVVDKESEDYK 624

Query: 814 LIEKYLVTTHAP---THMDWGLELEEVFSLEREG--EYDKFAPFRNKLENKMLLWHGSRL 873
           ++ KYL TT+ P   + +++G+ ++ VF++E +     D       KL NK+LLW GSR 
Sbjct: 625 MVVKYLETTYEPVKVSDVEYGVSVQNVFAVESDAIPSLDDI----KKLPNKVLLWCGSRS 684

Query: 874 TNFVGILSQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTDRKNPVGFMVLSEVAL 933
           +N +  + +G   A    P  GYMFG+ I  +D  +++A+Y +T    P GF+VL+  +L
Sbjct: 685 SNLLRHIYKGFLPAVCSLPVPGYMFGRAIVCSDAAAEAARYGFTAVDRPEGFLVLAVASL 744

Query: 934 GEVYEVKKAEYMERPPRG-------KHSTKGMGKKVPAVSEHVKWREDVVVPCGKPVASN 980
           GE     +      PP         K   KG+G+K    SEH  WR+D+ VPCG+ V S 
Sbjct: 745 GE-----EVTEFTSPPEDTKTLEDKKIGVKGLGRKKTEESEHFMWRDDIKVPCGRLVPSE 804

BLAST of MC06g1531 vs. TAIR 10
Match: AT3G14890.2 (phosphoesterase )

HSP 1 Score: 86.3 bits (212), Expect = 1.5e-16
Identity = 75/282 (26.60%), Postives = 119/282 (42.20%), Query Frame = 0

Query: 7   PWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKAKQIKSV 66
           P   EYAKS+RSSC++C   I  ++LR G ++   +    M  W+H  C    ++ I SV
Sbjct: 49  PVVAEYAKSNRSSCRSCSNKIAVKSLRLG-LISKGRGGVDMTRWHHFDCFPTDSESIASV 108

Query: 67  DDVEGLDSLRWEDQQKIRKYVEDSAAAAA---------------------VADTPVECGI 126
           DD++GL +L  EDQ  + K VE      A                      A T  +   
Sbjct: 109 DDIQGLSALEKEDQDALTKLVEQCGKVPAKKPDEKKGKAKKHIMGPKGLTKAATSSKVIA 168

Query: 127 EVAQASRAACKLCKQKIMKGEVRLSTAL-DGKGAKGLAWHHANCY-MEQSPSTQVEKLAG 186
           + A++SR++C  C Q I+  ++R+     D +G     WHH  C+ ++  P   VE + G
Sbjct: 169 DNAKSSRSSCNRCSQTIVSKDLRVGLVTEDSRGFDITRWHHLGCFPIDFHPIDSVEDIGG 228

Query: 187 WQNLPASDQAAVSALIKKLPSTENNEPRQAA-------SKVGKRK----------KDATG 246
           + +L   DQ  +  L + +   +  +    A       +K GK            + A  
Sbjct: 229 YSSLEKGDQMELKYLAEDVQKMDEGDDEAIADNELTEETKKGKHSPVAKLVEQPGEPAKE 288

Query: 247 DQDSKVTKAVGDVSVSRSMKTASDSPDSQNPSSLASKLEAQT 249
           D+D +  K   D    +  K   +SPDS    S  +K    T
Sbjct: 289 DEDEESKKPASDEISEQKTKDVKNSPDSSKVISEYAKSSRST 329

BLAST of MC06g1531 vs. TAIR 10
Match: AT3G14890.1 (phosphoesterase )

HSP 1 Score: 79.3 bits (194), Expect = 1.9e-14
Identity = 75/298 (25.17%), Postives = 125/298 (41.95%), Query Frame = 0

Query: 7   PWKVEYAKSSRSSCKTCKKPIDKENLRFGKMVQATQFDGFMPMWNHASCILKKAKQIKSV 66
           P   EYAKS+RSSC++C   I  ++LR G ++   +    M  W+H  C    ++ I SV
Sbjct: 49  PVVAEYAKSNRSSCRSCSNKIAVKSLRLG-LISKGRGGVDMTRWHHFDCFPTDSESIASV 108

Query: 67  DDVEGLDSLRWEDQQKIRKYVEDSAAAAA---------------------VADTPVECGI 126
           DD++GL +L  EDQ  + K VE      A                      A T  +   
Sbjct: 109 DDIQGLSALEKEDQDALTKLVEQCGKVPAKKPDEKKGKAKKHIMGPKGLTKAATSSKVIA 168

Query: 127 EVAQASRAACKLCKQKIMKGEVRLSTAL-DGKGAKGLAWHHANCY-MEQSPSTQVEKLAG 186
           + A++SR++C  C Q I+  ++R+     D +G     WHH  C+ ++  P   VE + G
Sbjct: 169 DNAKSSRSSCNRCSQTIVSKDLRVGLVTEDSRGFDITRWHHLGCFPIDFHPIDSVEDIGG 228

Query: 187 WQNLPASDQAAVSALIKKLPSTENNEPRQAASKVGKRKKDATGDQDSKVTKAVGDVSVSR 246
           + +L   DQ  +  L +              +K  K   D     D    +A+ D  ++ 
Sbjct: 229 YSSLEKGDQMELKYLAE-------------VNKKDKTLIDDVQKMDEGDDEAIADNELTE 288

Query: 247 SMKTASDSPDSQNPSSLASKLEAQTKELWKLKDDLKKHVTNAELREMLESNGQDSTGS 282
             K    SP       +A  +E   +   + +D+  K   + E+ E    + ++S  S
Sbjct: 289 ETKKGKHSP-------VAKLVEQPGEPAKEDEDEESKKPASDEISEQKTKDVKNSPDS 325