MC06g1520 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g1520
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	inhibitor of trypsin and hageman factor-like
Location	MC06: 22507054 .. 22507245 (+)
RNA-Seq Expression	MC06g1520
Synteny	MC06g1520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAGACAAGAATGGCCAGAACTGGTTGGAGTTAAAGTGAGCGTTGCTGCACTCATCATAAAGAGGGAGAATGCTAAGTTAGAGCCGACTGTGCTATTAGCAGGATCTCCAGTGACTTTAGATTACAGACCATATCGAGTTCGGATTTTTAAGAATATAGAGGATGTGGTGGTTGGGATTCCTAGAACTGGT

mRNA sequence

GGAAGACAAGAATGGCCAGAACTGGTTGGAGTTAAAGTGAGCGTTGCTGCACTCATCATAAAGAGGGAGAATGCTAAGTTAGAGCCGACTGTGCTATTAGCAGGATCTCCAGTGACTTTAGATTACAGACCATATCGAGTTCGGATTTTTAAGAATATAGAGGATGTGGTGGTTGGGATTCCTAGAACTGGT

Coding sequence (CDS)

GGAAGACAAGAATGGCCAGAACTGGTTGGAGTTAAAGTGAGCGTTGCTGCACTCATCATAAAGAGGGAGAATGCTAAGTTAGAGCCGACTGTGCTATTAGCAGGATCTCCAGTGACTTTAGATTACAGACCATATCGAGTTCGGATTTTTAAGAATATAGAGGATGTGGTGGTTGGGATTCCTAGAACTGGT

Protein sequence

GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPRTG

Homology

[Close]

BLAST of MC06g1520 vs. ExPASy Swiss-Prot
Match: P08454 (Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 2.0e-10
Identity = 34/63 (53.97%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 1   GRQEWPELVGVKVSVAALIIKRENAKL-EPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVG 60
           G+Q WPEL+GV   +A  II++EN+ +    +LL GSPVT+DYR  RVR+F NI   VV 
Sbjct: 43  GKQRWPELIGVPTKLAKGIIEKENSLITNVQILLNGSPVTMDYRCNRVRLFDNILGDVVQ 102

Query: 61  IPR 63
           IPR
Sbjct: 103 IPR 105

[Close]

BLAST of MC06g1520 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.3e-10
Identity = 35/65 (53.85%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 2  RQEWPELVGVKVSVAALIIKRENAKLE--PTVLLAGSPVTLDYRPYRVRIFKNIEDVVVG 61
          ++ WPEL+GV    A  II++EN+KL   P+V L GSPVT D+R  RVR+F N+ D VV 
Sbjct: 31 KETWPELIGVPAKFAREIIQKENSKLTNVPSV-LNGSPVTKDFRCERVRLFVNVLDFVVQ 90

Query: 62 IPRTG 65
          IPR G
Sbjct: 91 IPRVG 94

[Close]

BLAST of MC06g1520 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.2e-09
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          G+  WP LVGV  SVA  II+R+N  ++  +L  G+PVT D+R  RVRI+ N   +VV  
Sbjct: 5  GKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSP 64

Query: 61 PRTG 65
          PR G
Sbjct: 65 PRIG 68

[Close]

BLAST of MC06g1520 vs. ExPASy Swiss-Prot
Match: Q00783 (Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.7e-09
Identity = 33/65 (50.77%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 1   GRQEWPELVGVKVSVAALIIKRENAKLEPT-VLLAGSPVTLDYRPYRVRIFKNIEDVVVG 60
           G+  WPEL+GV   +A  II++EN+ +    +LL GSPVTLD R  RVR+F NI   VV 
Sbjct: 43  GKLRWPELIGVPTKLAKGIIEKENSLISNVHILLNGSPVTLDIRCDRVRLFDNILGYVVD 102

Query: 61  IPRTG 65
           IP  G
Sbjct: 103 IPVVG 107

[Close]

BLAST of MC06g1520 vs. ExPASy Swiss-Prot
Match: P05118 (Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.8e-09
Identity = 31/62 (50.00%), Postives = 42/62 (67.74%), Query Frame = 0


Query: 1   GRQEWPELVGVKVSVAALIIKRENAKL-EPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVG 60
           G+Q WPEL+GV   +A  II++EN  +    +LL+GSP+TLDY   RVR+F NI   VV 
Sbjct: 47  GKQMWPELIGVPTKLAKEIIEKENPSITNIPILLSGSPITLDYLCDRVRLFDNILGFVVQ 106

Query: 61  IP 62
           +P
Sbjct: 107 MP 108

[Close]

BLAST of MC06g1520 vs. NCBI nr
Match: XP_022146026.1 (inhibitor of trypsin and hageman factor-like [Momordica charantia])

HSP 1 Score: 130 bits (328), Expect = 4.94e-38
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI
Sbjct: 16 GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 75

Query: 61 PRTG 64
          PRTG
Sbjct: 76 PRTG 79

[Close]

BLAST of MC06g1520 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 3.49e-17
Identity = 39/64 (60.94%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          G+  WPELV VK SVA   I+R+   ++P VLLAGSPVT D RP RVRIF N+ D VV +
Sbjct: 12 GKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEV 71

Query: 61 PRTG 64
          PRTG
Sbjct: 72 PRTG 75

[Close]

BLAST of MC06g1520 vs. NCBI nr
Match: KZN03237.1 (hypothetical protein DCAR_011993 [Daucus carota subsp. sativus])

HSP 1 Score: 71.6 bits (174), Expect = 1.36e-14
Identity = 37/65 (56.92%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 1  GRQE-WPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVG 60
          GR+E WPELVG K  VAA  I++EN ++   V+L GSPVTLD  PYRVR+F N   +VV 
Sbjct: 11 GRKEAWPELVGEKGDVAAAKIEKENRRVHAVVVLEGSPVTLDLNPYRVRVFVNKYGLVVD 70

Query: 61 IPRTG 64
           PR G
Sbjct: 71 PPRIG 75

[Close]

BLAST of MC06g1520 vs. NCBI nr
Match: KAG6571499.1 (Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 70.5 bits (171), Expect = 3.60e-14
Identity = 34/65 (52.31%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPT-VLLAGSPVTLDYRPYRVRIFKNIEDVVVG 60
          G+  WPELVG++  +   IIK+EN  +    + LAGSPV LD+  +RVR+F NI DVVV 
Sbjct: 8  GKNRWPELVGIEARIVEKIIKKENPNVGIVQIFLAGSPVPLDFSCHRVRLFVNINDVVVS 67

Query: 61 IPRTG 64
          IP TG
Sbjct: 68 IPWTG 72

[Close]

BLAST of MC06g1520 vs. NCBI nr
Match: MQL67652.1 (hypothetical protein [Streptomyces vinaceus])

HSP 1 Score: 69.3 bits (168), Expect = 1.30e-13
Identity = 35/65 (53.85%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVL-LAGSPVTLDYRPYRVRIFKNIEDVVVG 60
          G+Q WPELVGV   VA   I++EN KL   V+ L G+P+TLDYR  RVRIF N   +V+ 
Sbjct: 17 GKQSWPELVGVPGKVAKETIQKENPKLTQVVIVLIGTPITLDYRFDRVRIFVNYLGIVIQ 76

Query: 61 IPRTG 64
          +PR G
Sbjct: 77 VPRVG 81

[Close]

BLAST of MC06g1520 vs. ExPASy TrEMBL
Match: A0A6J1CY53 (inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=LOC111015335 PE=3 SV=1)

HSP 1 Score: 130 bits (328), Expect = 2.39e-38
Identity = 64/64 (100.00%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI
Sbjct: 16 GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 75

Query: 61 PRTG 64
          PRTG
Sbjct: 76 PRTG 79

[Close]

BLAST of MC06g1520 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.69e-17
Identity = 39/64 (60.94%), Postives = 46/64 (71.88%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          G+  WPELV VK SVA   I+R+   ++P VLLAGSPVT D RP RVRIF N+ D VV +
Sbjct: 12 GKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVTEDLRPNRVRIFVNMNDRVVEV 71

Query: 61 PRTG 64
          PRTG
Sbjct: 72 PRTG 75

[Close]

BLAST of MC06g1520 vs. ExPASy TrEMBL
Match: A0A161Y246 (Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_011993 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 6.57e-15
Identity = 37/65 (56.92%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 1  GRQE-WPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVG 60
          GR+E WPELVG K  VAA  I++EN ++   V+L GSPVTLD  PYRVR+F N   +VV 
Sbjct: 11 GRKEAWPELVGEKGDVAAAKIEKENRRVHAVVVLEGSPVTLDLNPYRVRVFVNKYGLVVD 70

Query: 61 IPRTG 64
           PR G
Sbjct: 71 PPRIG 75

[Close]

BLAST of MC06g1520 vs. ExPASy TrEMBL
Match: A0A0A0L9Z4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142410 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.39e-13
Identity = 34/60 (56.67%), Postives = 40/60 (66.67%), Query Frame = 0


Query: 5  WPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIPRTG 64
          WPELVGV  S AA II+ EN  ++ T +L GSPV L+Y P RV +  N ED VV IPR G
Sbjct: 12 WPELVGVDFSAAARIIETENPNVKATKILIGSPVILNYDPTRVWVICNTEDKVVDIPRVG 71

[Close]

BLAST of MC06g1520 vs. ExPASy TrEMBL
Match: A0A0V0GN13 (Putative type I serine protease inhibitor-like OS=Solanum chacoense OX=4108 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 2.70e-13
Identity = 33/65 (50.77%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 1   GRQEWPELVGVKVSVAALIIKRENAKLEPT-VLLAGSPVTLDYRPYRVRIFKNIEDVVVG 60
           G+Q WPEL+GV   +A  II++EN  +    ++L+G+P+TLDYR  RVR+F NI  VVV 
Sbjct: 47  GKQFWPELIGVPTKLAKEIIEKENPSINDVRIVLSGTPITLDYRCNRVRLFDNILGVVVQ 106

Query: 61  IPRTG 64
           IPR  
Sbjct: 107 IPRVA 111

[Close]

BLAST of MC06g1520 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 60.8 bits (146), Expect = 4.5e-10
Identity = 28/63 (44.44%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 2  RQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGIP 61
          +  WPEL G     AA++I+REN  +   V+L GSPVT D+R  RVR+F +   +VV  P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

Query: 62 RTG 65
          ++G
Sbjct: 68 KSG 70

[Close]

BLAST of MC06g1520 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 50.1 bits (118), Expect = 7.9e-07
Identity = 26/64 (40.62%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          G+  WPEL+G     AA +IK EN+ L   V+  G+ VT D   YRVR++ +   +VV  
Sbjct: 10 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 69

Query: 61 PRTG 65
          P  G
Sbjct: 70 PTAG 73

[Close]

BLAST of MC06g1520 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 50.1 bits (118), Expect = 7.9e-07
Identity = 26/64 (40.62%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          G+  WPEL+G     AA +IK EN+ L   V+  G+ VT D   YRVR++ +   +VV  
Sbjct: 25 GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRN 84

Query: 61 PRTG 65
          P  G
Sbjct: 85 PTAG 88

[Close]

BLAST of MC06g1520 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.1e-05
Identity = 24/64 (37.50%), Postives = 37/64 (57.81%), Query Frame = 0


Query: 1  GRQEWPELVGVKVSVAALIIKRENAKLEPTVLLAGSPVTLDYRPYRVRIFKNIEDVVVGI 60
          G+  WPEL+G K   A  +I+REN K++  ++L G+ V   +   RV ++ N   +VV I
Sbjct: 36 GKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQI 95

Query: 61 PRTG 65
          P  G
Sbjct: 96 PIIG 99

The following BLAST results are available for this feature:

BLAST of MC06g1520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P08454	2.0e-10	53.97	Wound-induced proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=1 SV=2	[more]
Q02214	3.3e-10	53.85	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P19873	2.2e-09	48.44	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q00783	3.7e-09	50.77	Proteinase inhibitor 1 OS=Solanum tuberosum OX=4113 PE=3 SV=1	[more]
P05118	4.8e-09	50.00	Wound-induced proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 GN=PIIF PE=...	[more]

BLAST of MC06g1520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146026.1	4.94e-38	100.00	inhibitor of trypsin and hageman factor-like [Momordica charantia]	[more]
XP_011654473.1	3.49e-17	60.94	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
KZN03237.1	1.36e-14	56.92	hypothetical protein DCAR_011993 [Daucus carota subsp. sativus]	[more]
KAG6571499.1	3.60e-14	52.31	Proteinase inhibitor I-B, partial [Cucurbita argyrosperma subsp. sororia]	[more]
MQL67652.1	1.30e-13	53.85	hypothetical protein [Streptomyces vinaceus]	[more]

BLAST of MC06g1520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CY53	2.39e-38	100.00	inhibitor of trypsin and hageman factor-like OS=Momordica charantia OX=3673 GN=L...	[more]
A0A0A0KPI0	1.69e-17	60.94	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A161Y246	6.57e-15	56.92	Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_011993 ...	[more]
A0A0A0L9Z4	1.39e-13	56.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142410 PE=3 SV=1	[more]
A0A0V0GN13	2.70e-13	50.77	Putative type I serine protease inhibitor-like OS=Solanum chacoense OX=4108 PE=3...	[more]

BLAST of MC06g1520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	4.5e-10	44.44	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.1	7.9e-07	40.63	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	7.9e-07	40.63	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	1.1e-05	37.50	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 4..17 score: 50.0 coord: 41..51 score: 52.17 coord: 29..40 score: 45.65
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 3..64 e-value: 1.8E-18 score: 66.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 2..64
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 5..16
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..64 e-value: 1.8E-20 score: 75.0
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 2..64
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 2..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g1520.1	MC06g1520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity