MC06g1518 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC06g1518
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Proteinase inhibitor
Location	MC06: 22492183 .. 22492368 (+)
RNA-Seq Expression	MC06g1518
Synteny	MC06g1518

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCATGGCCAGAACTGGTGTTTGTTGATGCAAGTGTTGTGAGCAACATCATAAAGAAAGATCATCCTGATTTCAAGATAATTGTGCTGTTAGCAGGAACTCCAATTACCACAAAAGAATTGAGGTTCGACCGGGTTCGACTTTTTGTTAATATAAACAATGTTGTGGTTGAAACTCCCATCAATGGT

mRNA sequence

TCATGGCCAGAACTGGTGTTTGTTGATGCAAGTGTTGTGAGCAACATCATAAAGAAAGATCATCCTGATTTCAAGATAATTGTGCTGTTAGCAGGAACTCCAATTACCACAAAAGAATTGAGGTTCGACCGGGTTCGACTTTTTGTTAATATAAACAATGTTGTGGTTGAAACTCCCATCAATGGT

Coding sequence (CDS)

TCATGGCCAGAACTGGTGTTTGTTGATGCAAGTGTTGTGAGCAACATCATAAAGAAAGATCATCCTGATTTCAAGATAATTGTGCTGTTAGCAGGAACTCCAATTACCACAAAAGAATTGAGGTTCGACCGGGTTCGACTTTTTGTTAATATAAACAATGTTGTGGTTGAAACTCCCATCAATGGT

Protein sequence

SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPING

Homology

[Close]

BLAST of MC06g1518 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 5.7e-07
Identity = 27/59 (45.76%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 60
          SWP LV V  SV   II++ +P+ K ++L  GTP+ TK+ R +RVR++VN   +VV  P
Sbjct: 8  SWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPV-TKDFRCNRVRIWVNKRGLVVSPP 65

[Close]

BLAST of MC06g1518 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 7.4e-07
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPELV  +  + + II+ ++ + K IV+  G PI T++L F+RVR+FV+ N VV + P 
Sbjct: 10 SWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPI-TQDLNFNRVRVFVDENRVVTQVPA 69

Query: 61 NG 63
           G
Sbjct: 70 IG 70

[Close]

BLAST of MC06g1518 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 9.7e-07
Identity = 30/59 (50.85%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 2   WPELVFVDASVVSNIIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 60
           WPEL+ V A     II+K++P    I +LL G+P+ TK+ R DRVRLFVNI   VV+ P
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPV-TKDFRCDRVRLFVNILGDVVQIP 108

[Close]

BLAST of MC06g1518 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.7e-06
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 0


Query: 1   SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 60
           SWPEL+   A     II+K++P    +  L      T++LR +RVRLFVN+ ++VV+TP
Sbjct: 58  SWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQTP 116

[Close]

BLAST of MC06g1518 vs. ExPASy Swiss-Prot
Match: Q03199 (Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 4.8e-06
Identity = 27/59 (45.76%), Postives = 40/59 (67.80%), Query Frame = 0


Query: 2   WPELVFVDASVVSNIIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 60
           WPEL+   A     II+K++P    +  +L GTP+ T++LR +RVRLFVN+ + VV+TP
Sbjct: 47  WPELLGTPAKFAMQIIQKENPKLTNVQTVLNGTPV-TEDLRCNRVRLFVNVLDFVVQTP 104

[Close]

BLAST of MC06g1518 vs. NCBI nr
Match: KAG6571807.1 (hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 75.5 bits (184), Expect = 3.05e-16
Identity = 37/62 (59.68%), Postives = 48/62 (77.42%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPEL  + AS V NIIK+ HP+F+I++LLAG+P+T K+L+  RVRLFVNI  +VVE P 
Sbjct: 7  SWPELNLMQASTVENIIKRQHPEFQIVILLAGSPVT-KDLKHGRVRLFVNILGIVVEIPR 66

Query: 61 NG 62
           G
Sbjct: 67 EG 67

[Close]

BLAST of MC06g1518 vs. NCBI nr
Match: KGN60477.1 (hypothetical protein Csa_001998 [Cucumis sativus])

HSP 1 Score: 71.6 bits (174), Expect = 1.13e-14
Identity = 35/62 (56.45%), Postives = 47/62 (75.81%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWP L F+DA  V+NIIKK++PDF+I +LLAG+P+T K+L+  RVRLF N+  +VV  P 
Sbjct: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGSPVT-KDLKHGRVRLFTNMEEIVVIVPQ 70

Query: 61 NG 62
           G
Sbjct: 71 EG 71

[Close]

BLAST of MC06g1518 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 69.7 bits (169), Expect = 7.23e-14
Identity = 36/62 (58.06%), Postives = 49/62 (79.03%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPELVFV +SV  + I++D PD K +VLLAG+P+T ++LR +RVR+FVN+N+ VVE P 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVT-EDLRPNRVRIFVNMNDRVVEVPR 74

Query: 61 NG 62
           G
Sbjct: 75 TG 75

[Close]

BLAST of MC06g1518 vs. NCBI nr
Match: ALD47589.1 (serine proteinase inhibitor [Cucumis metulifer])

HSP 1 Score: 60.5 bits (145), Expect = 3.02e-10
Identity = 35/59 (59.32%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 2  WPELVFVDASVVSNIIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
          WPELV + A+    II+KD+P   K+IVLLAGT  TTK++R DRV LFVNI   VVE P
Sbjct: 12 WPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTG-TTKDIRCDRVWLFVNIEEKVVEIP 69

[Close]

BLAST of MC06g1518 vs. NCBI nr
Match: KGN50838.1 (hypothetical protein Csa_004692 [Cucumis sativus])

HSP 1 Score: 60.1 bits (144), Expect = 4.29e-10
Identity = 35/62 (56.45%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 2  WPELVFVDASVVSNIIKKDHPDFK-IIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 61
          WPELV + A++   IIKKD+P+ + +IVLLAGT  TTK+ R DRV LF NI+  VVE P 
Sbjct: 12 WPELVGIKATIAEYIIKKDNPNVENVIVLLAGTG-TTKDFRSDRVWLFTNIDGRVVEMPT 71

[Close]

BLAST of MC06g1518 vs. ExPASy TrEMBL
Match: A0A0A0LIF6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 5.47e-15
Identity = 35/62 (56.45%), Postives = 47/62 (75.81%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWP L F+DA  V+NIIKK++PDF+I +LLAG+P+T K+L+  RVRLF N+  +VV  P 
Sbjct: 11 SWPALNFMDARTVANIIKKENPDFQIFILLAGSPVT-KDLKHGRVRLFTNMEEIVVIVPQ 70

Query: 61 NG 62
           G
Sbjct: 71 EG 71

[Close]

BLAST of MC06g1518 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.50e-14
Identity = 36/62 (58.06%), Postives = 49/62 (79.03%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPELVFV +SV  + I++D PD K +VLLAG+P+T ++LR +RVR+FVN+N+ VVE P 
Sbjct: 15 SWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVT-EDLRPNRVRIFVNMNDRVVEVPR 74

Query: 61 NG 62
           G
Sbjct: 75 TG 75

[Close]

BLAST of MC06g1518 vs. ExPASy TrEMBL
Match: A0A0M3SAG8 (Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.46e-10
Identity = 35/59 (59.32%), Postives = 42/59 (71.19%), Query Frame = 0


Query: 2  WPELVFVDASVVSNIIKKDHPDF-KIIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
          WPELV + A+    II+KD+P   K+IVLLAGT  TTK++R DRV LFVNI   VVE P
Sbjct: 12 WPELVGIKATTAEYIIRKDNPSVEKVIVLLAGTG-TTKDIRCDRVWLFVNIEEKVVEIP 69

[Close]

BLAST of MC06g1518 vs. ExPASy TrEMBL
Match: A0A0A0KMM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.08e-10
Identity = 35/62 (56.45%), Postives = 44/62 (70.97%), Query Frame = 0


Query: 2  WPELVFVDASVVSNIIKKDHPDFK-IIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 61
          WPELV + A++   IIKKD+P+ + +IVLLAGT  TTK+ R DRV LF NI+  VVE P 
Sbjct: 12 WPELVGIKATIAEYIIKKDNPNVENVIVLLAGTG-TTKDFRSDRVWLFTNIDGRVVEMPT 71

[Close]

BLAST of MC06g1518 vs. ExPASy TrEMBL
Match: A0A0A0KME7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 8.44e-10
Identity = 32/59 (54.24%), Postives = 46/59 (77.97%), Query Frame = 0


Query: 2  WPELVFVDASVVSNIIKKDHPDFK-IIVLLAGTPITTKELRFDRVRLFVNINNVVVETP 59
          WPELV V A+    IIKKD+P+ + ++VLLAG+  TT+++R DRV +FVNI+++VVE P
Sbjct: 12 WPELVGVKATTAKYIIKKDNPNVENVVVLLAGSG-TTEDIRCDRVWVFVNIHDLVVEVP 69

[Close]

BLAST of MC06g1518 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 53.5 bits (127), Expect = 6.9e-08
Identity = 25/62 (40.32%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 1  SWPELVFVDASVVSNIIKKDHPDFKIIVLLAGTPITTKELRFDRVRLFVNINNVVVETPI 60
          SWPEL   +    + +I++++P     V+L G+P+T  + R DRVR+FV+ N +VV+TP 
Sbjct: 10 SWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTA-DFRCDRVRVFVDGNRIVVKTPK 69

Query: 61 NG 63
          +G
Sbjct: 70 SG 70

The following BLAST results are available for this feature:

BLAST of MC06g1518 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	5.7e-07	45.76	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q6XNP7	7.4e-07	43.55	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P16231	9.7e-07	50.85	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]
P20076	3.7e-06	42.37	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
Q03199	4.8e-06	45.76	Proteinase inhibitor I-B OS=Nicotiana tabacum OX=4097 GN=TIMPA PE=2 SV=1	[more]

BLAST of MC06g1518 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6571807.1	3.05e-16	59.68	hypothetical protein SDJN03_28535, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN60477.1	1.13e-14	56.45	hypothetical protein Csa_001998 [Cucumis sativus]	[more]
XP_011654473.1	7.23e-14	58.06	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
ALD47589.1	3.02e-10	59.32	serine proteinase inhibitor [Cucumis metulifer]	[more]
KGN50838.1	4.29e-10	56.45	hypothetical protein Csa_004692 [Cucumis sativus]	[more]

BLAST of MC06g1518 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIF6	5.47e-15	56.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G914570 PE=3 SV=1	[more]
A0A0A0KPI0	3.50e-14	58.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A0M3SAG8	1.46e-10	59.32	Serine proteinase inhibitor OS=Cucumis metulifer OX=61886 PE=2 SV=1	[more]
A0A0A0KMM0	2.08e-10	56.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G285030 PE=3 SV=1	[more]
A0A0A0KME7	8.44e-10	54.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G286040 PE=3 SV=1	[more]

BLAST of MC06g1518 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	6.9e-08	40.32	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 1..61 e-value: 2.4E-16 score: 60.1
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 1..61
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 1..62 e-value: 7.1E-17 score: 63.5
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 1..61
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 1..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC06g1518.1	MC06g1518.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity