MC06g1466 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g1466 vs. ExPASy Swiss-Prot
Match: Q9SU50 (Trehalase OS=Arabidopsis thaliana OX=3702 GN=TRE1 PE=2 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 3.6e-196
Identity = 320/557 (57.45%), Postives = 418/557 (75.04%), Query Frame = 0

Query: 21  DRGPVVPVTDLVRFLERLQLVAFNSSGKLDF-DLKYYVDLSLK--YDLNSTELAFDAL-E 80
           D GPVV  T LV FL+R+Q  A  S  K    D K Y+DLSLK  Y L++ E AFD L  
Sbjct: 70  DSGPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTS 129

Query: 81  RAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLPKVENAEVRAWAFDIHN 140
            + +  VPVE L+ F++EYF GAG DL++ EP DFV  P+GFL  VEN EVR WA ++H 
Sbjct: 130 ESHDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHG 189

Query: 141 FWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDSYWIIRGLLASKMYDTA 200
            W+NLS R SD + +  D HTLLPLP+P ++PGSRFRE+YYWDSYW+I+GL+ S+M+ TA
Sbjct: 190 LWRNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTA 249

Query: 201 KGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTEDLEFVRTALPALIE 260
           KG+V NL+S++E +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T+D E VR A+P L++
Sbjct: 250 KGLVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLK 309

Query: 261 EHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEKLASKLVNNHEKQHLYR 320
           E++FWNSG H V +R+A G +H L RYYAMWN+PRPESS+ DE+ AS      EKQ  +R
Sbjct: 310 EYEFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHR 369

Query: 321 ELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKMELDISNLARAIGDYCTA 380
           ++ATAAESG DFS+RWMRD  + +T+AT+S++PVDLN F+LKMELDI+ + +  GD   +
Sbjct: 370 DIATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGS 429

Query: 381 DHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDARNQNQEIYASNFIPLW 440
           D F++AS  R++    +FWN + GQWLDYWL + S + + TW A NQN  ++ASNF P+W
Sbjct: 430 DRFVKASKAREKAFQTVFWNEKAGQWLDYWL-SSSGEESETWKAENQNTNVFASNFAPIW 489

Query: 441 IESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPNGWAPIQHMIVEGLARS 500
           I S  SD   +KKV+ +L+NSGL+  AGI TSL NSG+QWD PNGWAP Q MIV GL RS
Sbjct: 490 INSINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRS 549

Query: 501 GLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGGGGEYIPQTGFGWSNGV 560
            +KEA+ +AEDIA +W+++NY+ YK +G +HEK  V + G++GGGGEY+PQTGFGWSNGV
Sbjct: 550 SVKEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGV 609

Query: 561 VLAFLEEFGWPADQNID 574
           +LAFLEE+GWP+  +I+
Sbjct: 610 ILAFLEEYGWPSHLSIE 625

BLAST of MC06g1466 vs. ExPASy Swiss-Prot
Match: Q9FWC1 (Probable trehalase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0521000 PE=2 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 1.1e-189
Identity = 321/568 (56.51%), Postives = 411/568 (72.36%), Query Frame = 0

Query: 15  LLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLSLKYDLNSTELAFDA 74
           + P AA  G  V    L+  L+R+Q  A  + G  DFD K YVDL L  D +    A  A
Sbjct: 1   MAPTAAVAGGGVEAEALLGLLQRVQSEALRAFGPNDFDPKLYVDLPLAADAS----AAAA 60

Query: 75  LERAPNGSVPVEQLKTFIREYFGGAGNDLV-YSEPADFVPQPTGFLPKVENAEVRAWAFD 134
           L   P  +    +++ +I  YF  AG+DLV  ++P DF   P GFLP+VE AE RAWA +
Sbjct: 61  LASLPRAAPSRGEMEAYISRYFALAGSDLVAAADPPDFERDPPGFLPRVERAEARAWALE 120

Query: 135 IHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDSYWIIRGLLASKMY 194
           +H  WK+L+RR +  +  RPD HTLLPLP   VVPGSRFRE+YYWDSYW++RGLL SKMY
Sbjct: 121 VHALWKDLTRRVAPAVAARPDRHTLLPLPGRVVVPGSRFREVYYWDSYWVVRGLLVSKMY 180

Query: 195 DTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTEDLEFVRTALPA 254
           +TAK IV+NL+ ++E +G VLNGAR+YYTNRSQPPLLSSMV DIY+ T D+ FVR   P+
Sbjct: 181 ETAKDIVLNLVYLVEKYGFVLNGARSYYTNRSQPPLLSSMVLDIYMATGDMAFVRRVFPS 240

Query: 255 LIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEKLASKLVNNHEKQH 314
           L++EH FW S  H+VAV +  G  H+L RY AMWN+PRPES+ +DE+ ASKL +   K+ 
Sbjct: 241 LLKEHSFWMSEVHNVAVMDNHGRVHNLSRYQAMWNKPRPESATIDEEFASKL-STAAKEK 300

Query: 315 LYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKMELDISNLARAIGDY 374
            Y ++A+ AE+GWDFSSRWMRDSTD++TL TS I+PVDLN F+LKME DI+  A+ IG+ 
Sbjct: 301 FYHQVASTAETGWDFSSRWMRDSTDMTTLTTSCIIPVDLNTFILKMEQDIAFFAKLIGES 360

Query: 375 CTADHFLEASIVRKRTINAIFWNSEKGQWLDYWL-ENGSYKGAHTWDARNQNQEIYASNF 434
            T++ F EAS  R   I+++ WN++  QWLDYWL  +G+ +G + W + +QN+ I+ASNF
Sbjct: 361 TTSEIFSEASKARHNAIDSVLWNADMEQWLDYWLPTDGNCQGVYQWKSISQNRAIFASNF 420

Query: 435 IPLWIESFYS------DNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPNGWAPIQ 494
           +PLW+ + +S      D  +  +V++SL+ SGLL  AGIATSL N+G+QWDFPNGWAP+Q
Sbjct: 421 VPLWLNAQHSGLEQFVDEAKSVRVMRSLQKSGLLQPAGIATSLSNTGQQWDFPNGWAPLQ 480

Query: 495 HMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGGGGEYIP 554
           H+IVEGL RSG  EAR LAEDIA +W+RTNY AYK TG MHEKYDV  CG  GGGGEY P
Sbjct: 481 HLIVEGLLRSGSGEARELAEDIATRWVRTNYDAYKATGAMHEKYDVVTCGKSGGGGEYKP 540

Query: 555 QTGFGWSNGVVLAFLEEFGWPADQNIDC 575
           QTGFGWSNGV+L+FL+EFGWP D+ IDC
Sbjct: 541 QTGFGWSNGVILSFLDEFGWPQDKKIDC 563

BLAST of MC06g1466 vs. ExPASy Swiss-Prot
Match: O43280 (Trehalase OS=Homo sapiens OX=9606 GN=TREH PE=1 SV=2)

HSP 1 Score: 399.4 bits (1025), Expect = 6.7e-110
Identity = 214/523 (40.92%), Postives = 300/523 (57.36%), Query Frame = 0

Query: 48  KLDFDLKYYVDLSLKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSE 107
           KL  D K +VD+ L          F  L R  N S+P EQL+ F+ E+F   G +L    
Sbjct: 46  KLYQDDKQFVDMPLSIAPEQVLQTFTELSRDHNHSIPREQLQAFVHEHFQAKGQELQPWT 105

Query: 108 PADFVPQPTGFLPKVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVV 167
           PAD+   P  FL K+ +A++RAWA  +H  WK L ++   ++L  P+  +L+    P +V
Sbjct: 106 PADWKDSPQ-FLQKISDAKLRAWAGQLHQLWKKLGKKMKPEVLSHPERFSLIYSEHPFIV 165

Query: 168 PGSRFREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQP 227
           PG RF E YYWDSYW++ GLL S+M +T KG++ N + +++ +GHV NG R YY  RSQP
Sbjct: 166 PGGRFVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVKTYGHVPNGGRVYYLQRSQP 225

Query: 228 PLLSSMVYDIYL-RTEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAM 287
           PLL+ M+ D YL  T D  F++  +  L  E  FW     +V+V +  G N+ L RYY  
Sbjct: 226 PLLTLMM-DCYLTHTNDTAFLQENIETLALELDFWTKN-RTVSV-SLEGKNYLLNRYYVP 285

Query: 288 WNEPRPESSLLDEKLASKLVNNHEKQHLYRELATAAESGWDFSSRWM---RDSTDLSTLA 347
           +  PRPES   D +LA  L    +++ L+ EL   AESGWDFSSRW+    +   LS + 
Sbjct: 286 YGGPRPESYSKDVELADTLPEG-DREALWAELKAGAESGWDFSSRWLIGGPNPNSLSGIR 345

Query: 348 TSSILPVDLNAFVLKMELDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWL 407
           TS ++PVDLNAF+ + E  +SN    +G+   A  +      R   +N + W+ + G W 
Sbjct: 346 TSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKYRILRSQRLAALNTVLWDEQTGAWF 405

Query: 408 DYWLENGSYKGAHTWDARNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLC-N 467
           DY LE            + +N+E Y SN  PLW   F SD     K LK L ++ +L   
Sbjct: 406 DYDLEK-----------KKKNREFYPSNLTPLWAGCF-SDPGVADKALKYLEDNRILTYQ 465

Query: 468 AGIATSLINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKN 527
            GI TSL  +G+QWDFPN WAP+Q +++ GLA++ L+ A+ +A  +A  W+RTN+  Y  
Sbjct: 466 YGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQ 525

Query: 528 TGYMHEKYDVEKCGDFGGGGEYIPQTGFGWSNGVVLAFLEEFG 566
              M+EKYDV   G  GGGGEY  Q GFGW+NGVVL  L+ +G
Sbjct: 526 KSAMYEKYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYG 551

BLAST of MC06g1466 vs. ExPASy Swiss-Prot
Match: Q9JLT2 (Trehalase OS=Mus musculus OX=10090 GN=Treh PE=1 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.2e-106
Identity = 203/518 (39.19%), Postives = 292/518 (56.37%), Query Frame = 0

Query: 52  DLKYYVDLSLKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADF 111
           D K +VD+SL    +     F  L    N S+P EQL+ F++ +F   G +L    P D+
Sbjct: 47  DDKQFVDMSLATSPDEVLQKFSELATVHNHSIPKEQLQEFVQSHFQPVGQELQSWTPEDW 106

Query: 112 VPQPTGFLPKVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSR 171
              P  FL K+ +A +R WA ++H  WK L ++   ++L  P+  +L+    P +VPG R
Sbjct: 107 KDSPQ-FLQKISDANLRVWAEELHKIWKKLGKKMKAEVLSYPERSSLIYSKHPFIVPGGR 166

Query: 172 FREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLS 231
           F E YYWDSYW++ GLL S+M  T KG++ N + +++ +GH+ NG R YY  RSQPPLL+
Sbjct: 167 FVEFYYWDSYWVMEGLLLSEMASTVKGMLQNFLDLVKTYGHIPNGGRIYYLQRSQPPLLT 226

Query: 232 SMVYDIYLRTEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPR 291
            M+      T+D+ F++  +  L  E  FW     +V+V +  G ++ L RYY  +  PR
Sbjct: 227 LMMDRYVAHTKDVAFLQENIGTLASELDFWTVN-RTVSVVSG-GQSYVLNRYYVPYGGPR 286

Query: 292 PESSLLDEKLASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTD---LSTLATSSIL 351
           PES   D +LA+  V   +++ L+ EL   AESGWDFSSRW+    D   LS++ TS ++
Sbjct: 287 PESYRKDAELANS-VPEGDRETLWAELKAGAESGWDFSSRWLVGGPDPDLLSSIRTSKMV 346

Query: 352 PVDLNAFVLKMELDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLE 411
           P DLNAF+ + E  +SN    +G+   A  +      R   + A+ W+ +KG W DY LE
Sbjct: 347 PADLNAFLCQAEELMSNFYSRLGNDTEATKYRNLRAQRLAAMEAVLWDEQKGAWFDYDLE 406

Query: 412 NGSYKGAHTWDARNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLC-NAGIAT 471
            G            +N E Y SN  PLW   F SD     K LK L +S +L    GI T
Sbjct: 407 KG-----------KKNLEFYPSNLSPLWAGCF-SDPSVADKALKYLEDSKILTYQYGIPT 466

Query: 472 SLINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMH 531
           SL N+G+QWDFPN WAP+Q +++ GLA+S     + +A  +A  W++TN+  Y     M 
Sbjct: 467 SLRNTGQQWDFPNAWAPLQDLVIRGLAKSASPRTQEVAFQLAQNWIKTNFKVYSQKSAMF 526

Query: 532 EKYDVEKCGDFGGGGEYIPQTGFGWSNGVVLAFLEEFG 566
           EKYD+   G  GGGGEY  Q GFGW+NG+ L  L+ +G
Sbjct: 527 EKYDISNGGHPGGGGEYEVQEGFGWTNGLALMLLDRYG 548

BLAST of MC06g1466 vs. ExPASy Swiss-Prot
Match: P19813 (Trehalase OS=Oryctolagus cuniculus OX=9986 GN=TREH PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.2e-105
Identity = 203/518 (39.19%), Postives = 295/518 (56.95%), Query Frame = 0

Query: 52  DLKYYVDLSLKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADF 111
           D K +VD+ L    +    +F  L    N +VP EQL+ F++E+F   G +L    P D+
Sbjct: 50  DDKQFVDMPLSTAPDQVLQSFAELAATYNNTVPREQLEKFVQEHFQAVGQELESWTPGDW 109

Query: 112 VPQPTGFLPKVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSR 171
              P  FL K+ + ++RAWA  +H  WK L ++   ++L +P+  +L+    P +VPG R
Sbjct: 110 KESPQ-FLQKISDPKLRAWAEQLHLLWKKLGKKIKPEVLSQPERFSLIYSQHPFIVPGGR 169

Query: 172 FREIYYWDSYWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLS 231
           F E YYWDSYW++ GLL S+M +T KG++ N + ++  +GH+ NG R YY  RSQPPLL+
Sbjct: 170 FVEFYYWDSYWVMEGLLLSEMAETVKGMLQNFLDLVTAYGHIPNGGRVYYLQRSQPPLLT 229

Query: 232 SMVYDIYLRTEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPR 291
            M+      T DL F+R  +  L  E  FW     +++V ++ G +H+L RY+  +  PR
Sbjct: 230 LMMDRYVAHTGDLAFLRENIETLALELDFWAEN-RTISV-SSGGNSHTLNRYHVPYGGPR 289

Query: 292 PESSLLDEKLASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTD---LSTLATSSIL 351
           PES   D +LA  L      + L+ EL   AESGWDFSSRW+  S +   L ++ TS ++
Sbjct: 290 PESYSKDTELAHTLPEG-SWETLWAELKAGAESGWDFSSRWLVGSPNPDSLGSIRTSKLV 349

Query: 352 PVDLNAFVLKMELDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLE 411
           PVDLNAF+ + E  +S     +G+   A  +      R   + A+ W+ +KG W DY LE
Sbjct: 350 PVDLNAFLCQAEELLSGFYSRLGNESQATKYRNLRAQRIAALTALLWDEDKGAWFDYDLE 409

Query: 412 NGSYKGAHTWDARNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNA-GIAT 471
           N           + +N E Y SN  PLW   F SD     K L+ L++S +L +  GI T
Sbjct: 410 N-----------QKKNHEFYPSNLTPLWAGCF-SDPAIADKALQYLQDSQILNHRHGIPT 469

Query: 472 SLINSGEQWDFPNGWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMH 531
           SL N+G+QWDFPN WAP+Q +++ GLA+S     + +A  +A  W+RTN+  Y     M+
Sbjct: 470 SLQNTGQQWDFPNAWAPLQDLVIRGLAKSPSARTQEVAFQLAQNWIRTNFDVYSQRSAMY 529

Query: 532 EKYDVEKCGDFGGGGEYIPQTGFGWSNGVVLAFLEEFG 566
           EKYD+      GGGGEY  Q GFGW+NGV L  L+ +G
Sbjct: 530 EKYDISNAQP-GGGGEYEVQEGFGWTNGVALMLLDRYG 550

BLAST of MC06g1466 vs. NCBI nr
Match: XP_022145919.1 (probable trehalase [Momordica charantia])

HSP 1 Score: 1199 bits (3101), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS
Sbjct: 22  LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 81

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP
Sbjct: 82  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 141

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS
Sbjct: 142 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 201

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR
Sbjct: 202 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 261

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 300
           TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK
Sbjct: 262 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 321

Query: 301 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 360
           LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME
Sbjct: 322 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 381

Query: 361 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 420
           LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA
Sbjct: 382 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 441

Query: 421 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 480
           RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN
Sbjct: 442 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 501

Query: 481 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 540
           GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG
Sbjct: 502 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 561

Query: 541 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS
Sbjct: 562 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 599

BLAST of MC06g1466 vs. NCBI nr
Match: XP_038907056.1 (probable trehalase [Benincasa hispida] >XP_038907058.1 probable trehalase [Benincasa hispida])

HSP 1 Score: 1060 bits (2740), Expect = 0.0
Identity = 500/580 (86.21%), Postives = 539/580 (92.93%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           +PLL     TLPEV  PR  DRGPVVPVT+LV+FLERLQ+VA NS GKLDFDLKYYVDLS
Sbjct: 25  MPLLRPATATLPEVFSPRLVDRGPVVPVTNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LK+DL+STE AFDALER+ NGSV VE LKTFI +YF  AG DLVYSEP DFVPQP GFLP
Sbjct: 85  LKFDLDSTETAFDALERSSNGSVSVENLKTFITDYFDSAGTDLVYSEPVDFVPQPIGFLP 144

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVEN+EVRAWA DIHNFWKNLSRR SDDL+ RP++HTLLPLP+P VVPGSRFRE+YYWDS
Sbjct: 145 KVENSEVRAWALDIHNFWKNLSRRVSDDLIHRPETHTLLPLPEPVVVPGSRFREVYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGN--HSLCRYYAMWNEPRPESSLLD 300
           T DLEFVR ALPALI+EHKFWNSGFHS+ VRNAPGGN  HSL RYYAMWNEPRPESSLLD
Sbjct: 265 TGDLEFVRNALPALIKEHKFWNSGFHSITVRNAPGGNGNHSLSRYYAMWNEPRPESSLLD 324

Query: 301 EKLASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLK 360
           EKLASK VNN+EKQHLYRE+A+AAESGWDFSSRWMRDSTDLSTLAT+SILPVDLN F+LK
Sbjct: 325 EKLASKFVNNYEKQHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILK 384

Query: 361 MELDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTW 420
           MELDISNLARA+GDYCTA HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NG YKG HTW
Sbjct: 385 MELDISNLARAVGDYCTAKHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGLYKGTHTW 444

Query: 421 DARNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDF 480
           DARNQNQ +YASNFIPLWIESFYSD+RQ+KKVLKSLRNSGLLCNAGIATS+INSGEQWDF
Sbjct: 445 DARNQNQNVYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQWDF 504

Query: 481 PNGWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDF 540
           PNGWAPIQHMIVEGLARSGLKEARALAEDIA +WL+TNYVAYK+TGYMHEKYDV+KCGDF
Sbjct: 505 PNGWAPIQHMIVEGLARSGLKEARALAEDIAARWLQTNYVAYKHTGYMHEKYDVQKCGDF 564

Query: 541 GGGGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGGGEY+PQTGFGWSNGVVLAFLEEFGWP DQNIDC+L S
Sbjct: 565 GGGGEYVPQTGFGWSNGVVLAFLEEFGWPKDQNIDCHLTS 604

BLAST of MC06g1466 vs. NCBI nr
Match: XP_022145920.1 (probable trehalase [Momordica charantia])

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 493/570 (86.49%), Postives = 528/570 (92.63%), Query Frame = 0

Query: 4   LLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLSLKY 63
           LL PAMTLPE+   RAAD GPVVPVT+LV+FLERLQL A  S GKLDFDLKYYVDLSLK+
Sbjct: 25  LLPPAMTLPELPFHRAADGGPVVPVTNLVKFLERLQLAALTSFGKLDFDLKYYVDLSLKH 84

Query: 64  DLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLPKVE 123
           DLNSTE AFDAL R+PNGSVPVE+LKTFIREYFG AGND+VY EPADFVP P GFLPKVE
Sbjct: 85  DLNSTERAFDALRRSPNGSVPVEELKTFIREYFGSAGNDMVYLEPADFVPLPKGFLPKVE 144

Query: 124 NAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDSYWI 183
           NAEVRAWA DIH  WKNLSR  SDDLL RPD HTLLPLPKPCV+PGSRFRE YYWDSYWI
Sbjct: 145 NAEVRAWALDIHRLWKNLSRTASDDLLNRPDDHTLLPLPKPCVIPGSRFRETYYWDSYWI 204

Query: 184 IRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTED 243
           IRGLLASKM+DTAKGIVINLISMIE FGHVLNGAR YYTNRSQPPLLSSMVYDIYLRT+D
Sbjct: 205 IRGLLASKMHDTAKGIVINLISMIEKFGHVLNGARVYYTNRSQPPLLSSMVYDIYLRTQD 264

Query: 244 LEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEKLAS 303
           LEFVRTALPALI+EHKFWNSGFHS+ V+NAP GNHSLCRY+AMWNEPRPESSLLDEKLAS
Sbjct: 265 LEFVRTALPALIKEHKFWNSGFHSITVQNAPTGNHSLCRYFAMWNEPRPESSLLDEKLAS 324

Query: 304 KLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKMELDI 363
           KLVNNHEK+HLYRE+A+AAESGWDFS+RWMRD TDLSTLAT+SILPVDLN F+LKMELDI
Sbjct: 325 KLVNNHEKEHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILKMELDI 384

Query: 364 SNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDARNQ 423
           SNLARA+GD  TA+ FLEAS VRKRTI++IFWNSEKGQWLDYWL+N SY+GAHTWDARNQ
Sbjct: 385 SNLARAVGDSGTAEQFLEASSVRKRTIDSIFWNSEKGQWLDYWLDNSSYEGAHTWDARNQ 444

Query: 424 NQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPNGWA 483
           NQ IYASNFIPLWIESFYSD+ +LKKV+KSL+NSGLLC++GIATS+INSG+QWDFPNGWA
Sbjct: 445 NQVIYASNFIPLWIESFYSDSIRLKKVMKSLQNSGLLCDSGIATSMINSGQQWDFPNGWA 504

Query: 484 PIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGGGGE 543
           PIQHMIVEGL RSG KEARALAEDIA+KWLRTNYV YK TGYMHEKYDVEKCGDFG GGE
Sbjct: 505 PIQHMIVEGLVRSGSKEARALAEDIAVKWLRTNYVVYKKTGYMHEKYDVEKCGDFGTGGE 564

Query: 544 YIPQTGFGWSNGVVLAFLEEFGWPADQNID 573
           YI QTGFGWSNGVVLAFLEEFGWPADQNID
Sbjct: 565 YISQTGFGWSNGVVLAFLEEFGWPADQNID 594

BLAST of MC06g1466 vs. NCBI nr
Match: XP_004143867.2 (probable trehalase [Cucumis sativus] >KGN50019.1 hypothetical protein Csa_000235 [Cucumis sativus])

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 484/578 (83.74%), Postives = 533/578 (92.21%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           +PLL   A +LPEV  PR AD+GPV+PV +LV+FLER+Q+VA NS GKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERIQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LK+DLNST++AFDALER+ NGSV VE L+TFI  YF  AG DLVYS PADF P P GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGTDLVYSNPADFDPHPDGFLP 144

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVEN EVRAWAFDIHNFWKNLSRR SDDL+  PD+HTLLPLP+P VVPGSRFREIYYWDS
Sbjct: 145 KVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 300
           T DLEFVR +LPALI+EHKFWNSGFHS+ V+N   GNHSL RYYAMWNEPRPESSL+DEK
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSITVQNG-NGNHSLSRYYAMWNEPRPESSLVDEK 324

Query: 301 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 360
           +ASK VNN+EK+HLYRE+A+AAESGWDFSSRWMRDSTDLSTLAT+SILPVDLN F+LKME
Sbjct: 325 VASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILKME 384

Query: 361 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 420
           LDISNLARA+GDYCTA+HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NGSYKGAH+WD 
Sbjct: 385 LDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSWDV 444

Query: 421 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 480
           RNQNQ +YASNFIPLW+ESFYSD+RQ+KKVLKSLRNSGLLCNAGIATS+INSGEQWDFPN
Sbjct: 445 RNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQWDFPN 504

Query: 481 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 540
           GWAPIQHMIVEGLARS L EA+ALAEDIA +WLRTNYVAYK+TG+MHEKYDV+KCG FGG
Sbjct: 505 GWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGGFGG 564

Query: 541 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGEY+PQTGFGWSNGVVLAFLEEFGWP DQ IDCYLPS
Sbjct: 565 GGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of MC06g1466 vs. NCBI nr
Match: XP_008437392.1 (PREDICTED: probable trehalase [Cucumis melo])

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 487/578 (84.26%), Postives = 532/578 (92.04%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           +PLL   A +LPEV  PR AD+GPV+PV +LV+FLERLQ+VA NS GKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LK+DLNST++AFDALER+ NGSV VE L+TFI  YF  AG DLVYS P DF PQP GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 144

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVEN EVR WA DIHNFWKNLSRR SDDL+  PD+HTLLPLP+P VVPGSRFREIYYWDS
Sbjct: 145 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 300
           T DLEFVR +LPALI+EHKFWNSGFHSV V+N   GNHSL RYYAMWNEPRPESSL+DEK
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG-NGNHSLSRYYAMWNEPRPESSLVDEK 324

Query: 301 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 360
           LASK  NN+EK+HLYRE+A+AAESGWDFSSRWMRDSTDLSTLAT+SILPVDLN F+LKME
Sbjct: 325 LASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILKME 384

Query: 361 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 420
           LDISNLARA+GDYCTA+HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NGSYKGAH+W+A
Sbjct: 385 LDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSWNA 444

Query: 421 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 480
           RNQNQ IYASNFIPLWIESFYSD+RQ+KKVLKSLRNSGLLCNAGIATS+INSGEQWDFPN
Sbjct: 445 RNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDFPN 504

Query: 481 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 540
           GWAPIQHMIVEGLARS L EA+ALAEDIA +WLRTNYVAYK+TG+MHEKYDV+KCGDFGG
Sbjct: 505 GWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDFGG 564

Query: 541 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGEY+PQTGFGWSNGVVLAFLEEFGWP DQ IDCYLPS
Sbjct: 565 GGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of MC06g1466 vs. ExPASy TrEMBL
Match: A0A6J1CWN7 (Trehalase OS=Momordica charantia OX=3673 GN=LOC111015267 PE=3 SV=1)

HSP 1 Score: 1199 bits (3101), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS
Sbjct: 22  LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 81

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP
Sbjct: 82  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 141

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS
Sbjct: 142 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 201

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR
Sbjct: 202 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 261

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 300
           TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK
Sbjct: 262 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 321

Query: 301 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 360
           LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME
Sbjct: 322 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 381

Query: 361 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 420
           LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA
Sbjct: 382 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 441

Query: 421 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 480
           RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN
Sbjct: 442 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 501

Query: 481 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 540
           GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG
Sbjct: 502 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 561

Query: 541 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS
Sbjct: 562 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 599

BLAST of MC06g1466 vs. ExPASy TrEMBL
Match: A0A6J1CVU6 (Trehalase OS=Momordica charantia OX=3673 GN=LOC111015268 PE=3 SV=1)

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 493/570 (86.49%), Postives = 528/570 (92.63%), Query Frame = 0

Query: 4   LLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLSLKY 63
           LL PAMTLPE+   RAAD GPVVPVT+LV+FLERLQL A  S GKLDFDLKYYVDLSLK+
Sbjct: 25  LLPPAMTLPELPFHRAADGGPVVPVTNLVKFLERLQLAALTSFGKLDFDLKYYVDLSLKH 84

Query: 64  DLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLPKVE 123
           DLNSTE AFDAL R+PNGSVPVE+LKTFIREYFG AGND+VY EPADFVP P GFLPKVE
Sbjct: 85  DLNSTERAFDALRRSPNGSVPVEELKTFIREYFGSAGNDMVYLEPADFVPLPKGFLPKVE 144

Query: 124 NAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDSYWI 183
           NAEVRAWA DIH  WKNLSR  SDDLL RPD HTLLPLPKPCV+PGSRFRE YYWDSYWI
Sbjct: 145 NAEVRAWALDIHRLWKNLSRTASDDLLNRPDDHTLLPLPKPCVIPGSRFRETYYWDSYWI 204

Query: 184 IRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTED 243
           IRGLLASKM+DTAKGIVINLISMIE FGHVLNGAR YYTNRSQPPLLSSMVYDIYLRT+D
Sbjct: 205 IRGLLASKMHDTAKGIVINLISMIEKFGHVLNGARVYYTNRSQPPLLSSMVYDIYLRTQD 264

Query: 244 LEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEKLAS 303
           LEFVRTALPALI+EHKFWNSGFHS+ V+NAP GNHSLCRY+AMWNEPRPESSLLDEKLAS
Sbjct: 265 LEFVRTALPALIKEHKFWNSGFHSITVQNAPTGNHSLCRYFAMWNEPRPESSLLDEKLAS 324

Query: 304 KLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKMELDI 363
           KLVNNHEK+HLYRE+A+AAESGWDFS+RWMRD TDLSTLAT+SILPVDLN F+LKMELDI
Sbjct: 325 KLVNNHEKEHLYREIASAAESGWDFSTRWMRDPTDLSTLATTSILPVDLNVFILKMELDI 384

Query: 364 SNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDARNQ 423
           SNLARA+GD  TA+ FLEAS VRKRTI++IFWNSEKGQWLDYWL+N SY+GAHTWDARNQ
Sbjct: 385 SNLARAVGDSGTAEQFLEASSVRKRTIDSIFWNSEKGQWLDYWLDNSSYEGAHTWDARNQ 444

Query: 424 NQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPNGWA 483
           NQ IYASNFIPLWIESFYSD+ +LKKV+KSL+NSGLLC++GIATS+INSG+QWDFPNGWA
Sbjct: 445 NQVIYASNFIPLWIESFYSDSIRLKKVMKSLQNSGLLCDSGIATSMINSGQQWDFPNGWA 504

Query: 484 PIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGGGGE 543
           PIQHMIVEGL RSG KEARALAEDIA+KWLRTNYV YK TGYMHEKYDVEKCGDFG GGE
Sbjct: 505 PIQHMIVEGLVRSGSKEARALAEDIAVKWLRTNYVVYKKTGYMHEKYDVEKCGDFGTGGE 564

Query: 544 YIPQTGFGWSNGVVLAFLEEFGWPADQNID 573
           YI QTGFGWSNGVVLAFLEEFGWPADQNID
Sbjct: 565 YISQTGFGWSNGVVLAFLEEFGWPADQNID 594

BLAST of MC06g1466 vs. ExPASy TrEMBL
Match: A0A0A0KMF2 (Trehalase OS=Cucumis sativus OX=3659 GN=Csa_5G149880 PE=3 SV=1)

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 484/578 (83.74%), Postives = 533/578 (92.21%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           +PLL   A +LPEV  PR AD+GPV+PV +LV+FLER+Q+VA NS GKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERIQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LK+DLNST++AFDALER+ NGSV VE L+TFI  YF  AG DLVYS PADF P P GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFDSAGTDLVYSNPADFDPHPDGFLP 144

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVEN EVRAWAFDIHNFWKNLSRR SDDL+  PD+HTLLPLP+P VVPGSRFREIYYWDS
Sbjct: 145 KVENVEVRAWAFDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 300
           T DLEFVR +LPALI+EHKFWNSGFHS+ V+N   GNHSL RYYAMWNEPRPESSL+DEK
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSITVQNG-NGNHSLSRYYAMWNEPRPESSLVDEK 324

Query: 301 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 360
           +ASK VNN+EK+HLYRE+A+AAESGWDFSSRWMRDSTDLSTLAT+SILPVDLN F+LKME
Sbjct: 325 VASKFVNNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILKME 384

Query: 361 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 420
           LDISNLARA+GDYCTA+HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NGSYKGAH+WD 
Sbjct: 385 LDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSWDV 444

Query: 421 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 480
           RNQNQ +YASNFIPLW+ESFYSD+RQ+KKVLKSLRNSGLLCNAGIATS+INSGEQWDFPN
Sbjct: 445 RNQNQNVYASNFIPLWVESFYSDSRQMKKVLKSLRNSGLLCNAGIATSMINSGEQWDFPN 504

Query: 481 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 540
           GWAPIQHMIVEGLARS L EA+ALAEDIA +WLRTNYVAYK+TG+MHEKYDV+KCG FGG
Sbjct: 505 GWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGGFGG 564

Query: 541 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGEY+PQTGFGWSNGVVLAFLEEFGWP DQ IDCYLPS
Sbjct: 565 GGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of MC06g1466 vs. ExPASy TrEMBL
Match: A0A5A7TH01 (Trehalase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001860 PE=3 SV=1)

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 487/578 (84.26%), Postives = 532/578 (92.04%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           +PLL   A +LPEV  PR AD+GPV+PV +LV+FLERLQ+VA NS GKLDFDLKYYVDLS
Sbjct: 1   MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 60

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LK+DLNST++AFDALER+ NGSV VE L+TFI  YF  AG DLVYS P DF PQP GFLP
Sbjct: 61  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 120

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVEN EVR WA DIHNFWKNLSRR SDDL+  PD+HTLLPLP+P VVPGSRFREIYYWDS
Sbjct: 121 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 180

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 181 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 240

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 300
           T DLEFVR +LPALI+EHKFWNSGFHSV V+N   GNHSL RYYAMWNEPRPESSL+DEK
Sbjct: 241 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG-NGNHSLSRYYAMWNEPRPESSLVDEK 300

Query: 301 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 360
           LASK  NN+EK+HLYRE+A+AAESGWDFSSRWMRDSTDLSTLAT+SILPVDLN F+LKME
Sbjct: 301 LASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILKME 360

Query: 361 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 420
           LDISNLARA+GDYCTA+HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NGSYKGAH+W+A
Sbjct: 361 LDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSWNA 420

Query: 421 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 480
           RNQNQ IYASNFIPLWIESFYSD+RQ+KKVLKSLRNSGLLCNAGIATS+INSGEQWDFPN
Sbjct: 421 RNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDFPN 480

Query: 481 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 540
           GWAPIQHMIVEGLARS L EA+ALAEDIA +WLRTNYVAYK+TG+MHEKYDV+KCGDFGG
Sbjct: 481 GWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDFGG 540

Query: 541 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGEY+PQTGFGWSNGVVLAFLEEFGWP DQ IDCYLPS
Sbjct: 541 GGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 577

BLAST of MC06g1466 vs. ExPASy TrEMBL
Match: A0A1S3ATK4 (Trehalase OS=Cucumis melo OX=3656 GN=LOC103482823 PE=3 SV=1)

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 487/578 (84.26%), Postives = 532/578 (92.04%), Query Frame = 0

Query: 1   LPLLLLPAMTLPEVLLPRAADRGPVVPVTDLVRFLERLQLVAFNSSGKLDFDLKYYVDLS 60
           +PLL   A +LPEV  PR AD+GPV+PV +LV+FLERLQ+VA NS GKLDFDLKYYVDLS
Sbjct: 25  MPLLRPAAASLPEVFSPRLADKGPVIPVPNLVKFLERLQVVALNSFGKLDFDLKYYVDLS 84

Query: 61  LKYDLNSTELAFDALERAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLP 120
           LK+DLNST++AFDALER+ NGSV VE L+TFI  YF  AG DLVYS P DF PQP GFLP
Sbjct: 85  LKFDLNSTQIAFDALERSSNGSVSVENLRTFISNYFHSAGTDLVYSNPVDFNPQPDGFLP 144

Query: 121 KVENAEVRAWAFDIHNFWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDS 180
           KVEN EVR WA DIHNFWKNLSRR SDDL+  PD+HTLLPLP+P VVPGSRFREIYYWDS
Sbjct: 145 KVENVEVRTWALDIHNFWKNLSRRVSDDLIHHPDTHTLLPLPEPVVVPGSRFREIYYWDS 204

Query: 181 YWIIRGLLASKMYDTAKGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLR 240
           YWIIRGLLASKMYDTAKGIVINLISMI++FGHVLNGAR+YYTNRSQPPLLSSMVYDIYLR
Sbjct: 205 YWIIRGLLASKMYDTAKGIVINLISMIDEFGHVLNGARSYYTNRSQPPLLSSMVYDIYLR 264

Query: 241 TEDLEFVRTALPALIEEHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEK 300
           T DLEFVR +LPALI+EHKFWNSGFHSV V+N   GNHSL RYYAMWNEPRPESSL+DEK
Sbjct: 265 TGDLEFVRNSLPALIKEHKFWNSGFHSVTVQNG-NGNHSLSRYYAMWNEPRPESSLVDEK 324

Query: 301 LASKLVNNHEKQHLYRELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKME 360
           LASK  NN+EK+HLYRE+A+AAESGWDFSSRWMRDSTDLSTLAT+SILPVDLN F+LKME
Sbjct: 325 LASKFANNYEKKHLYREIASAAESGWDFSSRWMRDSTDLSTLATTSILPVDLNVFILKME 384

Query: 361 LDISNLARAIGDYCTADHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDA 420
           LDISNLARA+GDYCTA+HF EAS+VRK+TIN+IFWNSEKGQWLDYWL+NGSYKGAH+W+A
Sbjct: 385 LDISNLARAVGDYCTAEHFFEASLVRKKTINSIFWNSEKGQWLDYWLDNGSYKGAHSWNA 444

Query: 421 RNQNQEIYASNFIPLWIESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPN 480
           RNQNQ IYASNFIPLWIESFYSD+RQ+KKVLKSLRNSGLLCNAGIATS+INSGEQWDFPN
Sbjct: 445 RNQNQNIYASNFIPLWIESFYSDSRQMKKVLKSLRNSGLLCNAGIATSVINSGEQWDFPN 504

Query: 481 GWAPIQHMIVEGLARSGLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGG 540
           GWAPIQHMIVEGLARS L EA+ALAEDIA +WLRTNYVAYK+TG+MHEKYDV+KCGDFGG
Sbjct: 505 GWAPIQHMIVEGLARSELPEAKALAEDIAARWLRTNYVAYKHTGFMHEKYDVQKCGDFGG 564

Query: 541 GGEYIPQTGFGWSNGVVLAFLEEFGWPADQNIDCYLPS 578
           GGEY+PQTGFGWSNGVVLAFLEEFGWP DQ IDCYLPS
Sbjct: 565 GGEYVPQTGFGWSNGVVLAFLEEFGWPKDQKIDCYLPS 601

BLAST of MC06g1466 vs. TAIR 10
Match: AT4G24040.1 (trehalase 1 )

HSP 1 Score: 686.0 bits (1769), Expect = 2.6e-197
Identity = 320/557 (57.45%), Postives = 418/557 (75.04%), Query Frame = 0

Query: 21  DRGPVVPVTDLVRFLERLQLVAFNSSGKLDF-DLKYYVDLSLK--YDLNSTELAFDAL-E 80
           D GPVV  T LV FL+R+Q  A  S  K    D K Y+DLSLK  Y L++ E AFD L  
Sbjct: 70  DSGPVVATTKLVTFLQRVQHTALRSYPKKQTPDPKSYIDLSLKRPYSLSTIESAFDDLTS 129

Query: 81  RAPNGSVPVEQLKTFIREYFGGAGNDLVYSEPADFVPQPTGFLPKVENAEVRAWAFDIHN 140
            + +  VPVE L+ F++EYF GAG DL++ EP DFV  P+GFL  VEN EVR WA ++H 
Sbjct: 130 ESHDQPVPVETLEKFVKEYFDGAGEDLLHHEPVDFVSDPSGFLSNVENEEVREWAREVHG 189

Query: 141 FWKNLSRRTSDDLLKRPDSHTLLPLPKPCVVPGSRFREIYYWDSYWIIRGLLASKMYDTA 200
            W+NLS R SD + +  D HTLLPLP+P ++PGSRFRE+YYWDSYW+I+GL+ S+M+ TA
Sbjct: 190 LWRNLSCRVSDSVRESADRHTLLPLPEPVIIPGSRFREVYYWDSYWVIKGLMTSQMFTTA 249

Query: 201 KGIVINLISMIEDFGHVLNGARAYYTNRSQPPLLSSMVYDIYLRTEDLEFVRTALPALIE 260
           KG+V NL+S++E +G+ LNGARAYYTNRSQPPLLSSMVY+IY  T+D E VR A+P L++
Sbjct: 250 KGLVTNLMSLVETYGYALNGARAYYTNRSQPPLLSSMVYEIYNVTKDEELVRKAIPLLLK 309

Query: 261 EHKFWNSGFHSVAVRNAPGGNHSLCRYYAMWNEPRPESSLLDEKLASKLVNNHEKQHLYR 320
           E++FWNSG H V +R+A G +H L RYYAMWN+PRPESS+ DE+ AS      EKQ  +R
Sbjct: 310 EYEFWNSGKHKVVIRDANGYDHVLSRYYAMWNKPRPESSVFDEESASGFSTMLEKQRFHR 369

Query: 321 ELATAAESGWDFSSRWMRDSTDLSTLATSSILPVDLNAFVLKMELDISNLARAIGDYCTA 380
           ++ATAAESG DFS+RWMRD  + +T+AT+S++PVDLN F+LKMELDI+ + +  GD   +
Sbjct: 370 DIATAAESGCDFSTRWMRDPPNFTTMATTSVVPVDLNVFLLKMELDIAFMMKVSGDQNGS 429

Query: 381 DHFLEASIVRKRTINAIFWNSEKGQWLDYWLENGSYKGAHTWDARNQNQEIYASNFIPLW 440
           D F++AS  R++    +FWN + GQWLDYWL + S + + TW A NQN  ++ASNF P+W
Sbjct: 430 DRFVKASKAREKAFQTVFWNEKAGQWLDYWL-SSSGEESETWKAENQNTNVFASNFAPIW 489

Query: 441 IESFYSDNRQLKKVLKSLRNSGLLCNAGIATSLINSGEQWDFPNGWAPIQHMIVEGLARS 500
           I S  SD   +KKV+ +L+NSGL+  AGI TSL NSG+QWD PNGWAP Q MIV GL RS
Sbjct: 490 INSINSDENLVKKVVTALKNSGLIAPAGILTSLTNSGQQWDSPNGWAPQQEMIVTGLGRS 549

Query: 501 GLKEARALAEDIAMKWLRTNYVAYKNTGYMHEKYDVEKCGDFGGGGEYIPQTGFGWSNGV 560
            +KEA+ +AEDIA +W+++NY+ YK +G +HEK  V + G++GGGGEY+PQTGFGWSNGV
Sbjct: 550 SVKEAKEMAEDIARRWIKSNYLVYKKSGTIHEKLKVTELGEYGGGGEYMPQTGFGWSNGV 609

Query: 561 VLAFLEEFGWPADQNID 574
           +LAFLEE+GWP+  +I+
Sbjct: 610 ILAFLEEYGWPSHLSIE 625