MC06g1379 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1379
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAB hydrolase-1 domain-containing protein
LocationMC06: 20969811 .. 20974086 (+)
RNA-Seq ExpressionMC06g1379
SyntenyMC06g1379
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAGAATCCCCAATTCACCAATACGCAATTACATTCTTCTTCTTCATCTTCTTCTTCTTCTTCTTCTTCAGCTTCCAAAATATGCCGTTTATTGAGGTTAACAGCAGCACTGCAGGTTCGGAGAATGCAAGCATCAACTCATCAATCAATCTTTTCTACAGAACTTATGGACGCGGATCAACTAAGGTCCTTCTTATCATAGGTCTCTTCAATTTTTATTCTCCTGCTTTACCCTTTTCATTTCTGCTTTTCGGACTCCATGATTCTGATACCTCTAAAATCGATGAATCCGATGGGTAGGATTGGCCGGAACCCACGACTCGTGGTGCCCCCAAATCAAAGGCCTTTGCGGGACCGATGTGCCCAATGATGACGCCGATCGGGACGTCACCGGTGGCCGGAATGCGGCCGACGCCAGCCAAGAATCCAACTCCGGGGATGGGGGTGGGATCGAGGTTTGTGCATTTGATAACCGGGGCATGGGTCGGAGCTCCGTCCCCACCAAGAAATCTGAATATACGTGAGTTGTTTCATAATCATCAGAGCATTCTTTTATTTGTCTTTTTTTTTTAATATTGATTTATTTGTGGTTTATGAATCAATTTTCTTCCTTGCGCGTGTCTGAGAAAACAAATAAGAATTTTAGGGTGTTGGGTCTATTTTCTGTTCCCTTTCCTCCTTTACAAATTTGAGAATTTGTTTTACCACATCTTTTCTAATACCAAAAATGAAAAGAACTTCATTCTTATTTTCGTTTCTGGCAATTTTTTAGTAATCTGTTTTCCTTTTCTTCGTTGTGCAGAACAAAGATAATGGCCAAGGATGCCATTGCCTTATTGGATCATTTGGGGTGGGAAAAAGCTCATGTTTTTGGTCATTCCATGGGTGAGTGAGTTTTTAACTCTCTTCCATTTCATCACTTCTGAACAAGAAATAGTGAAAGTATCATCTTCTTATTGTTATTAACTCTTCGTTCTTTCATATGATCATCTGAATTTTCTCCCCTATATCAATAGGAGGTATGATAGCTTGCAAATTAGCAGCTATGGCGCCAGAAAGAGTCAAGTCATTGGCCATGCTCAATGTAACTGGTGGAGGTTTTCAATGTTGCCCCAAGGTATCTGAATAAATATTTATTACTGCCAAACTGAGTTGTTTATTTGTTATATAATCTGCAGAAAAATAGCATCAAACTGATAGAATGTTCTTATATTCAATCAGTGCCAGATACCATATGCAACATCAAGAAACAATATAATTCTCATTGACAAAAAAATGCTATTTTAGTTGTGGTGAAGCTGTTTCATAAGGAATAATGATAATCTCTTTCTTTTTACATTTTTCTTGTAGCTTGACAGGCAAACATTCGACATTGCAGTCAGATTCTTAAAGGCCAAGACTCCGGAGCAAAGGGCTAGTGTTGATTTGGATACCCACTATTCAAAGGTAACGAAAAGTTTTGGTGTTTATCTTCTGTTATCTTAAGTAGATTAATAATTGTTAAACATTGTAATACATGTAGACTGTATCTTTTTCTAAGAAATATTGTTGTATCTTATGGTGCAGGAATATCTTGAGGAGTTTGTGGGATTTGAAAAGAGAAGAGCAGTTTTATATCGGGTATGTATGATGGGAGGATCACTCTCAACTGGCTTTATGTACCTTCATAGTTGGATTCTGGATTCTCTTATCTTTTTAATAAGCCCGATCCTACCCCGTTGCCGCTCCCTCTTTTCAAACCACCCTCTCCTCCCTAATCCTCCCAAAAAAAGGAGGGAAAAGGGCAACAATAAGTTCTTTTTTTTTTAAGGCTTCTAAAACTTACTAATGGCGTTCAAATGTAGGGTTGGATTTTTTGCCATCTGTCAACTAGTTAATTCTACCATTATAAGTTGCCTCAGATTTTACTGATCTCTACAGTCTGTAGATAGTTTGGAACCGGCAATATCCTATCATAAAGATAAACATACTTCTGTTTAATGATACAAATCTATATCTACGCACATAGAAACCCAAATACTCATTCGATTGTTTCTGAGATTTGTTTTTCTTTAAGTAAACCTGATATTGAATAACAACAACCAAGTATTTTTTGGCAATGAGCGTTACATTTGGAATCTAACCATGAAGGAAAAGGAGGAGTCCAACATCCCCTCTTACGACAAACTTTTTAAAAACTTCCTCGCAAAAACTACTTTAATATGCAAAGCACTAAGTTACAAAGAACTAATGTCAGTTGTAAGGAGACCTATTTGAGAAAGGTCCATCTTCTCCCCGTTAATCAAGCTCCTTGCCACCACATTTCTGTAGTTATTTATTGAATCCCTATTCTTTAGCATAAATAATTCCCTGTGAATAGCTAGTTGTTTCCGAGATTTGTCAAAGTCGTGAAATCACATGTAGTTGTGTATGTATAAAGGGATGTATGTCTAGATAGGACTCTGCCAAAATGGATGCATTTTTCTTCAAACCATGGCCTCACATTGAGAGGCATCTTAATATTTGAGGAGCTTATTAATAGTTTCAGGTGTATTTGACAGTCTTCATCTATTGATCGTTTATATCAATTCCTTCTGTCTCTATTAACCGTTTTTCTGTTGGCAAACAAATTTGGATCCTTGGTCTCGGAACTTCTTTCCCCAAAATAATATATTATTTGGATCCTCCGGTATATAATCATTACACTCACACTCGCACATCACGCATAATACATTCATAAAGATTTAGAGCCCAAGTCAACCTGAAAAACCATTGCAGGAATATGTTAAAGGTATATCATCAACAGGAATGCAGTCTAATTATGGTTTTGTGGGTCAAGTCTATGCATGCTGGATGCATAATGTGACAAGAAAAGAGATCGAATGCCTTCGCTCTGCTGGAATTCTTGTATCAGTCATTCATGGCAGGTAAACAATCATGTCTCTTATTAGCTATCCAAATCTGCAAAGTAAGTTTGTGGAGATAAACATGTTTCTAACACCCACCATTTATTGCCAATGTTGAGCTATGGGCATAGCTCAACAGTAAAGGGATTTACTCGATCAGTAGTTCAAATCCCCACACTTCCACATTTATTGTAAAAAGAAACCTGCCGTTTATTGCATAAAGGTATTTTACCTTCTTGTTGATTGATGATTGTTTGATTTTCGATGACTAGGCACGATGTAATTGCTCAAATATTCTATGCTAGACAACTTGCAGAAAGACTGTATCCAGTTGCGAGAATGGTAGATCTTCACGGAGGTCATCTAGTAAGTCGCGAGAGAACCAAAGAGGTATATATATTTTTATCCGAATTGGAATACATCTAAATTGTACTTTTCACTATGTCTAGTTGACGACAGAAAATGACAGCTGAAGTGTTTGATTTCTACAGGTAAACCAAGCTCTTCTTGACCTAATCAGGGCCTCAGAAACAAAGATGAATCCACATGACTGGACCAACTTGCCAAAGAAAAGCTCATGTGAGCATTTTTTTCTAGTAGTCCTCTGTTTTTCTTTGTTCCTTTGCAAATGGTGAAGAAAATATCTTCTTTCTAACAGGGTGGATGGAGGAGAGAATGTCATTAGTAACACTGAAGACTGAAGCAGGAAGCAGTGTCTCCTCCAAACCTTTCTTGTTGGAGAGACTACATCTTTGCTTTTTGTATCTCCTTGGTCTGATTCTAATGGCGTTCGAGTATCTCCGAAACGCCTTCAAGAGCTTGAAATCAGTCCGAGTCGGAGCCTCCATTTCATGAACAAGCATTCCATTCCAAGGACTCTAAATTTTTACAGGTGAGTTGTTTTTTTTATTGTCCTCTATTTCCACAAAGAACTCAACAAAAAGAGGAATCTCAAGGTTGGTTTTGGTTTCAACTTTGTTGTTTCAGGGTCTGTCTGTTCATTTTTTCTTGTGATGGGAAATAACCAAATTATCAAAGTGAAGTTGGGGAATGAGTTTTCTTACAATACTGAGGACTTCTTAGATAAACACAAGGGAGAGAAGCCAACGAAAGGCCAATCTTAAAGGTTGGTGATCCCTCCCCTCTTGTAATCTGTATCTAACACACCTGCTACTGTTAACTACTTACCCTTTTGTATAATTTATAAAAATCTTCATTTTATACATTAATTGCATATTGTTCTCAGTTTAAAGTCGTGTTGTATTCTAATCGCTCCATGAACTTTTAAAGTTGTGTCTATCAGGTTTTGTACAAAGTTTGTAATTGATCCATCAACTTTCAATTTTATGTCAACAACTCACTAGGTTAAATTTGTTAGTTTATTGTGTCAT

mRNA sequence

CCAGAATCCCCAATTCACCAATACGCAATTACATTCTTCTTCTTCATCTTCTTCTTCTTCTTCTTCTTCAGCTTCCAAAATATGCCGTTTATTGAGGTTAACAGCAGCACTGCAGGTTCGGAGAATGCAAGCATCAACTCATCAATCAATCTTTTCTACAGAACTTATGGACGCGGATCAACTAAGGTCCTTCTTATCATAGGATTGGCCGGAACCCACGACTCGTGGTGCCCCCAAATCAAAGGCCTTTGCGGGACCGATGTGCCCAATGATGACGCCGATCGGGACGTCACCGGTGGCCGGAATGCGGCCGACGCCAGCCAAGAATCCAACTCCGGGGATGGGGGTGGGATCGAGGTTTGTGCATTTGATAACCGGGGCATGGGTCGGAGCTCCGTCCCCACCAAGAAATCTGAATATACAACAAAGATAATGGCCAAGGATGCCATTGCCTTATTGGATCATTTGGGGTGGGAAAAAGCTCATGTTTTTGGTCATTCCATGGGAGGTATGATAGCTTGCAAATTAGCAGCTATGGCGCCAGAAAGAGTCAAGTCATTGGCCATGCTCAATGTAACTGGTGGAGGTTTTCAATGTTGCCCCAAGCTTGACAGGCAAACATTCGACATTGCAGTCAGATTCTTAAAGGCCAAGACTCCGGAGCAAAGGGCTAGTGTTGATTTGGATACCCACTATTCAAAGGAATATCTTGAGGAGTTTGTGGGATTTGAAAAGAGAAGAGCAGTTTTATATCGGGAATATGTTAAAGGTATATCATCAACAGGAATGCAGTCTAATTATGGTTTTGTGGGTCAAGTCTATGCATGCTGGATGCATAATGTGACAAGAAAAGAGATCGAATGCCTTCGCTCTGCTGGAATTCTTGTATCAGTCATTCATGGCAGGCACGATGTAATTGCTCAAATATTCTATGCTAGACAACTTGCAGAAAGACTGTATCCAGTTGCGAGAATGGTAGATCTTCACGGAGGTCATCTAGTAAGTCGCGAGAGAACCAAAGAGGTAAACCAAGCTCTTCTTGACCTAATCAGGGCCTCAGAAACAAAGATGAATCCACATGACTGGACCAACTTGCCAAAGAAAAGCTCATGGTGGATGGAGGAGAGAATGTCATTAGTAACACTGAAGACTGAAGCAGGAAGCAGTGTCTCCTCCAAACCTTTCTTGTTGGAGAGACTACATCTTTGCTTTTTGTATCTCCTTGGTCTGATTCTAATGGCGTTCGAGTATCTCCGAAACGCCTTCAAGAGCTTGAAATCAGTCCGAGTCGGAGCCTCCATTTCATGAACAAGCATTCCATTCCAAGGACTCTAAATTTTTACAGGGTCTGTCTGTTCATTTTTTCTTGTGATGGGAAATAACCAAATTATCAAAGTGAAGTTGGGGAATGAGTTTTCTTACAATACTGAGGACTTCTTAGATAAACACAAGGGAGAGAAGCCAACGAAAGGCCAATCTTAAAGGTTGGTGATCCCTCCCCTCTTGTAATCTGTATCTAACACACCTGCTACTGTTAACTACTTACCCTTTTGTATAATTTATAAAAATCTTCATTTTATACATTAATTGCATATTGTTCTCAGTTTAAAGTCGTGTTGTATTCTAATCGCTCCATGAACTTTTAAAGTTGTGTCTATCAGGTTTTGTACAAAGTTTGTAATTGATCCATCAACTTTCAATTTTATGTCAACAACTCACTAGGTTAAATTTGTTAGTTTATTGTGTCAT

Coding sequence (CDS)

ATGCCGTTTATTGAGGTTAACAGCAGCACTGCAGGTTCGGAGAATGCAAGCATCAACTCATCAATCAATCTTTTCTACAGAACTTATGGACGCGGATCAACTAAGGTCCTTCTTATCATAGGATTGGCCGGAACCCACGACTCGTGGTGCCCCCAAATCAAAGGCCTTTGCGGGACCGATGTGCCCAATGATGACGCCGATCGGGACGTCACCGGTGGCCGGAATGCGGCCGACGCCAGCCAAGAATCCAACTCCGGGGATGGGGGTGGGATCGAGGTTTGTGCATTTGATAACCGGGGCATGGGTCGGAGCTCCGTCCCCACCAAGAAATCTGAATATACAACAAAGATAATGGCCAAGGATGCCATTGCCTTATTGGATCATTTGGGGTGGGAAAAAGCTCATGTTTTTGGTCATTCCATGGGAGGTATGATAGCTTGCAAATTAGCAGCTATGGCGCCAGAAAGAGTCAAGTCATTGGCCATGCTCAATGTAACTGGTGGAGGTTTTCAATGTTGCCCCAAGCTTGACAGGCAAACATTCGACATTGCAGTCAGATTCTTAAAGGCCAAGACTCCGGAGCAAAGGGCTAGTGTTGATTTGGATACCCACTATTCAAAGGAATATCTTGAGGAGTTTGTGGGATTTGAAAAGAGAAGAGCAGTTTTATATCGGGAATATGTTAAAGGTATATCATCAACAGGAATGCAGTCTAATTATGGTTTTGTGGGTCAAGTCTATGCATGCTGGATGCATAATGTGACAAGAAAAGAGATCGAATGCCTTCGCTCTGCTGGAATTCTTGTATCAGTCATTCATGGCAGGCACGATGTAATTGCTCAAATATTCTATGCTAGACAACTTGCAGAAAGACTGTATCCAGTTGCGAGAATGGTAGATCTTCACGGAGGTCATCTAGTAAGTCGCGAGAGAACCAAAGAGGTAAACCAAGCTCTTCTTGACCTAATCAGGGCCTCAGAAACAAAGATGAATCCACATGACTGGACCAACTTGCCAAAGAAAAGCTCATGGTGGATGGAGGAGAGAATGTCATTAGTAACACTGAAGACTGAAGCAGGAAGCAGTGTCTCCTCCAAACCTTTCTTGTTGGAGAGACTACATCTTTGCTTTTTGTATCTCCTTGGTCTGATTCTAATGGCGTTCGAGTATCTCCGAAACGCCTTCAAGAGCTTGAAATCAGTCCGAGTCGGAGCCTCCATTTCATGA

Protein sequence

MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTDVPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAKDAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNYGFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVDLHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAGSSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS
Homology
BLAST of MC06g1379 vs. ExPASy Swiss-Prot
Match: A8IAD8 (Putative aminoacrylate hydrolase RutD OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571) OX=438753 GN=rutD PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 4.6e-12
Identity = 54/172 (31.40%), Postives = 76/172 (44.19%), Query Frame = 0

Query: 23  NLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTDVPNDDADRDVTGGRNAADASQE 82
           +L+Y  +G G T +LL  G+ G    W PQI+ L                          
Sbjct: 10  DLYYEIHGAG-TPILLSAGMGGGAGFWRPQIEALAARH---------------------- 69

Query: 83  SNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAKDAIALLDHLGWEKAHVFGHSMG 142
                    +V  +D+ G GRS         T   MA+D   +LD  G E AHV GH++G
Sbjct: 70  ---------QVILYDHAGTGRSGRDIGPRSITE--MARDMARVLDAAGVEDAHVAGHAIG 129

Query: 143 GMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPE 195
           G+I  +LA  APERV+SL ++N    G+       R+ F++  R L A  PE
Sbjct: 130 GIIGMELALAAPERVRSLTIVN----GWARADGFLRRCFEVRKRILLASGPE 143

BLAST of MC06g1379 vs. ExPASy Swiss-Prot
Match: B7KWT4 (Putative aminoacrylate hydrolase RutD OS=Methylorubrum extorquens (strain CM4 / NCIMB 13688) OX=440085 GN=rutD PE=3 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 7.3e-10
Identity = 41/120 (34.17%), Postives = 59/120 (49.17%), Query Frame = 0

Query: 93  VCAFDNRGMGRSSVPTKKSEYTTKIMAKDAIALLDHLGWEKAHVFGHSMGGMIACKLAAM 152
           V  +D+RG GRS  P +   +    MA+D +ALLDHLG   A + GH++GG+IA +LA  
Sbjct: 44  VVTYDHRGTGRSPSPLEPG-HDIAAMARDVLALLDHLGIGTADIVGHALGGLIALQLALT 103

Query: 153 APERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLEE 212
            PERV  + ++N    G+       R+ F      L+   PE          Y   +L E
Sbjct: 104 HPERVGRIVVIN----GWAAMDPATRRCFAARKALLRHAGPEAFVRAQAIFLYPAPWLSE 158

BLAST of MC06g1379 vs. ExPASy Swiss-Prot
Match: B1M5I5 (Putative aminoacrylate hydrolase RutD OS=Methylobacterium radiotolerans (strain ATCC 27329 / DSM 1819 / JCM 2831 / NBRC 15690 / NCIMB 10815 / 0-1) OX=426355 GN=rutD PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.1e-09
Identity = 64/235 (27.23%), Postives = 104/235 (44.26%), Query Frame = 0

Query: 93  VCAFDNRGMGRSSVPTKKS-EYTTKIMAKDAIALLDHLGWEKAHVFGHSMGGMIACKLAA 152
           V  +D+RG GRS  P + + ++    MA+DA A+LD  G E+A V GH++GG+IA ++A 
Sbjct: 43  VVTYDHRGTGRS--PGRLAPDHDVPAMARDARAVLDAAGIERADVVGHALGGLIALQMAL 102

Query: 153 MAPERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLE 212
            AP+RV  + ++N    G+       R+ F      L    PE          Y   +L 
Sbjct: 103 DAPDRVGRVVVIN----GWDALDPATRRCFAARRAILAGGGPEAFVRAQAIFLYPAAWLS 162

Query: 213 ---EFVGFEKRRAVLYREYVKGISSTGMQSNYGFVGQVYACWMHNVTRKEIECLRSAGIL 272
              E V  ++ +A+ +    + +  T + +  GF                 + L + G  
Sbjct: 163 AQAERVEADEAQALAHFPGAETV-LTRIAALEGFA--------------IADRLGAIGHE 222

Query: 273 VSVIHGRHDVIAQIFYARQLAERLYPVARMVDLHGGHLVSRERTKEVNQALLDLI 324
             V+  R DV+     + +LA  L      +   GGH  S  R +  N+ALLD +
Sbjct: 223 TLVMAARDDVLVPYTRSERLAAALPNARLALAPEGGHAHSVTRPEAFNRALLDFL 256

BLAST of MC06g1379 vs. ExPASy Swiss-Prot
Match: B1ZB18 (Putative aminoacrylate hydrolase RutD OS=Methylorubrum populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) OX=441620 GN=rutD PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.8e-09
Identity = 40/120 (33.33%), Postives = 57/120 (47.50%), Query Frame = 0

Query: 93  VCAFDNRGMGRSSVPTKKSEYTTKIMAKDAIALLDHLGWEKAHVFGHSMGGMIACKLAAM 152
           V  +D+RG GRS  P +   +    MA+D +ALLDHLG     + GH++GG+IA  LA  
Sbjct: 44  VVTYDHRGTGRSPGPLEPG-HDIAAMARDVLALLDHLGIGTTDIVGHALGGLIALHLALT 103

Query: 153 APERVKSLAMLNVTGGGFQCCPKLDRQTFDIAVRFLKAKTPEQRASVDLDTHYSKEYLEE 212
            PERV+ + ++N    G+       R+ F      L    PE          Y   +L E
Sbjct: 104 HPERVERIVVIN----GWAVMDPATRRCFSARKALLTQAGPEAFVRAQAIFLYPAPWLSE 158

BLAST of MC06g1379 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 3.6e-09
Identity = 30/75 (40.00%), Postives = 43/75 (57.33%), Query Frame = 0

Query: 90  GIEVCAFDNRGMGRSSVPTKKSEYTTKIMAKDAIALLDHLGWEKAHVFGHSMGGMIACKL 149
           G  V A D +G G SS P +  EY  +++ K+ +  LD LG  +A   GH  GGM+   +
Sbjct: 285 GYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYM 344

Query: 150 AAMAPERVKSLAMLN 165
           A   PERV+++A LN
Sbjct: 345 ALFYPERVRAVASLN 359

BLAST of MC06g1379 vs. NCBI nr
Match: XP_022158048.1 (uncharacterized protein LOC111024626 [Momordica charantia])

HSP 1 Score: 815 bits (2106), Expect = 6.08e-298
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD
Sbjct: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK
Sbjct: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD
Sbjct: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG
Sbjct: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
           SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS
Sbjct: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408

BLAST of MC06g1379 vs. NCBI nr
Match: XP_038875890.1 (putative aminoacrylate hydrolase RutD [Benincasa hispida])

HSP 1 Score: 696 bits (1797), Expect = 5.92e-251
Identity = 346/408 (84.80%), Postives = 372/408 (91.18%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIEV++ST  +E++ INS+I +FYRTYG GSTKVLLIIGLAGTHDSW PQIKGL GTD
Sbjct: 1   MPFIEVHTSTTAAEDSKINSAIKIFYRTYGCGSTKVLLIIGLAGTHDSWGPQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPNDD DR      NAA   +ES   DGGGIEVCAFDNRGMGRSSVPTKKSEYTT+IMAK
Sbjct: 61  VPNDDGDR------NAAHVREESTFEDGGGIEVCAFDNRGMGRSSVPTKKSEYTTQIMAK 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKAH+FGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHIFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEE++GFEKRR VLYREYVKGIS+TGMQSN 
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEYIGFEKRRTVLYREYVKGISATGMQSND 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VTRKEIECL+SAG LVSVIHGRHDVIAQ++YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHTVTRKEIECLQSAGFLVSVIHGRHDVIAQMYYARRLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLIRASETKM+PHDWTNLPKKSSWWMEERM+ VTLKTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIRASETKMSPHDWTNLPKKSSWWMEERMAFVTLKTEGG 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
           SS+SSKPFLLERLHL FLY+ GLIL+ FEYL N  KSLK VRVGAS++
Sbjct: 361 SSISSKPFLLERLHLFFLYIFGLILLVFEYLWNTLKSLKPVRVGASLT 402

BLAST of MC06g1379 vs. NCBI nr
Match: XP_023532415.1 (uncharacterized protein LOC111794595 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 689 bits (1779), Expect = 4.24e-248
Identity = 342/409 (83.62%), Postives = 372/409 (90.95%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIE+N+ST G+E+A IN++I +FYRTYGRGSTKVLLIIGLAGTHDSW PQIKGL GTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPND  +R+  G + AAD  +E++  DGGGIEVCAFDNRGMGRSS PTKKSEYTTKIMA+
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEK HVFGHSMGGMIACKLAA+APERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFE RRAVLYREYVKGIS+TGMQS+Y
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQSDY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VT KEIECL+SAG LVSVIHGR DVIAQI+YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGRQDVIAQIYYARKLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLIRASETKM+P DWTNLPKKSSWWMEERMSL+TLKTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIRASETKMSPQDWTNLPKKSSWWMEERMSLITLKTEGG 360

Query: 361 -SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
            S+VS++PFLLERLH  FLYL GLIL  FEYLR+ FKSLK  RVG S++
Sbjct: 361 GSTVSTQPFLLERLHFFFLYLFGLILFMFEYLRDTFKSLKPARVGPSLT 409

BLAST of MC06g1379 vs. NCBI nr
Match: XP_022958692.1 (uncharacterized protein LOC111459843 [Cucurbita moschata])

HSP 1 Score: 687 bits (1774), Expect = 2.45e-247
Identity = 342/409 (83.62%), Postives = 372/409 (90.95%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIE+N+ST G+E+A IN++I +FYRTYGRGSTKVLLIIGLAGTHDSW  QIKGL GTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNLQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPND  +R+  G + AAD  +E++  DGGGIEVCAFDNRGMGRSS PTKKSEYTTKIMA+
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEK HVFGHSMGGMIACKLAA+APERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFE RRAVLYREYVKGIS+TGMQ++Y
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VT KEIECL+SAG LVSVIHGR DVIAQI+YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGRQDVIAQIYYARKLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLIRASETKM+P DWTNLPKKSSWWMEERMSL+TLKTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIRASETKMSPQDWTNLPKKSSWWMEERMSLITLKTEGG 360

Query: 361 -SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
            S+VS+KPFLLERLHL FLYL GLIL  FEYLR+ FKSLK  RVG S++
Sbjct: 361 GSTVSTKPFLLERLHLFFLYLFGLILFMFEYLRDTFKSLKPARVGPSLT 409

BLAST of MC06g1379 vs. NCBI nr
Match: XP_004145933.1 (uncharacterized protein LOC101203096 [Cucumis sativus] >KGN49859.1 hypothetical protein Csa_000422 [Cucumis sativus])

HSP 1 Score: 685 bits (1768), Expect = 1.55e-246
Identity = 337/408 (82.60%), Postives = 368/408 (90.20%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIEVN+S+  +E+  INS+I +FYRTYGRGSTKVLLIIGLAGTHDSW PQIKGL GTD
Sbjct: 1   MPFIEVNTSSTAAEDVKINSAIKIFYRTYGRGSTKVLLIIGLAGTHDSWGPQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPNDD DR      NAA   +E    DGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK
Sbjct: 61  VPNDDGDR------NAAHVGEEPTFEDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKAH+FGHSMGGMIACKL AMAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHIFGHSMGGMIACKLGAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRF+KAKTPEQRASVDLDTHYSKEYLEE+VGFEKRR +LYREYV+GIS+TGMQSN 
Sbjct: 181 FDIAVRFMKAKTPEQRASVDLDTHYSKEYLEEYVGFEKRRTLLYREYVEGISATGMQSND 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VTRK+IECL+SAG LVSVIHGRHDVIAQ++YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHQVTRKDIECLQSAGFLVSVIHGRHDVIAQMYYARRLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLI+ASETKM+PHDWTNLPKKSSWWMEERM+ VT+KTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIKASETKMSPHDWTNLPKKSSWWMEERMAFVTMKTEGG 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
           SS+SSKPFLLE+LHL FLY  GLIL+ F +LRN  KSLK  RVGA+++
Sbjct: 361 SSISSKPFLLEKLHLFFLYFFGLILLIFGHLRNTIKSLKPTRVGATLT 402

BLAST of MC06g1379 vs. ExPASy TrEMBL
Match: A0A6J1DV10 (uncharacterized protein LOC111024626 OS=Momordica charantia OX=3673 GN=LOC111024626 PE=4 SV=1)

HSP 1 Score: 815 bits (2106), Expect = 2.94e-298
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD
Sbjct: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK
Sbjct: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD
Sbjct: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG
Sbjct: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
           SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS
Sbjct: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408

BLAST of MC06g1379 vs. ExPASy TrEMBL
Match: A0A6J1H2I9 (uncharacterized protein LOC111459843 OS=Cucurbita moschata OX=3662 GN=LOC111459843 PE=4 SV=1)

HSP 1 Score: 687 bits (1774), Expect = 1.19e-247
Identity = 342/409 (83.62%), Postives = 372/409 (90.95%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIE+N+ST G+E+A IN++I +FYRTYGRGSTKVLLIIGLAGTHDSW  QIKGL GTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNLQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPND  +R+  G + AAD  +E++  DGGGIEVCAFDNRGMGRSS PTKKSEYTTKIMA+
Sbjct: 61  VPNDHGNRNTVGNQIAADDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEK HVFGHSMGGMIACKLAA+APERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHVFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFE RRAVLYREYVKGIS+TGMQ++Y
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VT KEIECL+SAG LVSVIHGR DVIAQI+YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGRQDVIAQIYYARKLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLIRASETKM+P DWTNLPKKSSWWMEERMSL+TLKTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIRASETKMSPQDWTNLPKKSSWWMEERMSLITLKTEGG 360

Query: 361 -SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
            S+VS+KPFLLERLHL FLYL GLIL  FEYLR+ FKSLK  RVG S++
Sbjct: 361 GSTVSTKPFLLERLHLFFLYLFGLILFMFEYLRDTFKSLKPARVGPSLT 409

BLAST of MC06g1379 vs. ExPASy TrEMBL
Match: A0A0A0KLW7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139370 PE=4 SV=1)

HSP 1 Score: 685 bits (1768), Expect = 7.49e-247
Identity = 337/408 (82.60%), Postives = 368/408 (90.20%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIEVN+S+  +E+  INS+I +FYRTYGRGSTKVLLIIGLAGTHDSW PQIKGL GTD
Sbjct: 1   MPFIEVNTSSTAAEDVKINSAIKIFYRTYGRGSTKVLLIIGLAGTHDSWGPQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPNDD DR      NAA   +E    DGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK
Sbjct: 61  VPNDDGDR------NAAHVGEEPTFEDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKAH+FGHSMGGMIACKL AMAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHIFGHSMGGMIACKLGAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRF+KAKTPEQRASVDLDTHYSKEYLEE+VGFEKRR +LYREYV+GIS+TGMQSN 
Sbjct: 181 FDIAVRFMKAKTPEQRASVDLDTHYSKEYLEEYVGFEKRRTLLYREYVEGISATGMQSND 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VTRK+IECL+SAG LVSVIHGRHDVIAQ++YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHQVTRKDIECLQSAGFLVSVIHGRHDVIAQMYYARRLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLI+ASETKM+PHDWTNLPKKSSWWMEERM+ VT+KTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIKASETKMSPHDWTNLPKKSSWWMEERMAFVTMKTEGG 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
           SS+SSKPFLLE+LHL FLY  GLIL+ F +LRN  KSLK  RVGA+++
Sbjct: 361 SSISSKPFLLEKLHLFFLYFFGLILLIFGHLRNTIKSLKPTRVGATLT 402

BLAST of MC06g1379 vs. ExPASy TrEMBL
Match: A0A6J1K3F3 (uncharacterized protein LOC111491334 OS=Cucurbita maxima OX=3661 GN=LOC111491334 PE=4 SV=1)

HSP 1 Score: 683 bits (1762), Expect = 7.97e-246
Identity = 338/409 (82.64%), Postives = 370/409 (90.46%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIE+N+ST G+E+A IN++I +FYRTYGRGSTKVLLIIGLAGTHDSW PQIKGL GTD
Sbjct: 1   MPFIEINTSTTGAEDAKINTAIKIFYRTYGRGSTKVLLIIGLAGTHDSWNPQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           VPND  +R+  G + AA   +E++  DGGGIEVCAFDNRGMGRSS PTKKSEYTTKIMA+
Sbjct: 61  VPNDHGNRNTVGNQIAAGDGEEASFEDGGGIEVCAFDNRGMGRSSAPTKKSEYTTKIMAQ 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEK H+FGHSMGGMIACKLAA+APERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKVHIFGHSMGGMIACKLAAIAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFE RRAVLYREYVKGIS+TGMQ++Y
Sbjct: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFETRRAVLYREYVKGISATGMQTDY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VT KEIECL+SAG LVSVIHGR DVIAQI+YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHKVTNKEIECLQSAGFLVSVIHGRQDVIAQIYYARKLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLIRASETKM+P DWTNLPKK+SWWMEERMSL+TLKTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIRASETKMSPQDWTNLPKKNSWWMEERMSLITLKTEGG 360

Query: 361 -SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
            S+VS++PFLLERLH  FLYL GLIL  FEYL N FKSLK  RVG S++
Sbjct: 361 GSTVSTQPFLLERLHFFFLYLFGLILFMFEYLWNTFKSLKPARVGPSLT 409

BLAST of MC06g1379 vs. ExPASy TrEMBL
Match: A0A1S3AUJ9 (putative aminoacrylate hydrolase RutD OS=Cucumis melo OX=3656 GN=LOC103482970 PE=4 SV=1)

HSP 1 Score: 681 bits (1758), Expect = 2.50e-245
Identity = 336/408 (82.35%), Postives = 367/408 (89.95%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPFIEVN+S+  +E+  INS+I +FYRT+GRGSTKVLLIIGLAGTHDSW PQIKGL GTD
Sbjct: 1   MPFIEVNTSSTAAEDVKINSAIKIFYRTFGRGSTKVLLIIGLAGTHDSWGPQIKGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
           + NDD DR      NA+  S E    DGGGIEVCAFDNRGMGRSSVPTKKSEYTT IMAK
Sbjct: 61  ISNDDDDR------NASHVSAEPTFQDGGGIEVCAFDNRGMGRSSVPTKKSEYTTNIMAK 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           DAIALLDHLGWEKAH+FGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT
Sbjct: 121 DAIALLDHLGWEKAHIFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
           FDIAVRF+KAKTPEQRASVDLDTHYSKEYLEE+VGFEKRR +LYREYV+GIS+TGMQSN 
Sbjct: 181 FDIAVRFMKAKTPEQRASVDLDTHYSKEYLEEYVGFEKRRTLLYREYVEGISATGMQSND 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQV ACW H VTRK+IECL+SAG LVSVIHGRHDVIAQ++YAR+LAE+LYPVARMVD
Sbjct: 241 GFAGQVNACWTHKVTRKDIECLQSAGFLVSVIHGRHDVIAQMYYARRLAEKLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVSRERT+EVNQALLDLIRASETKM+PHDWTNLPKKSSWWMEERM+ VT+KTE G
Sbjct: 301 LHGGHLVSRERTEEVNQALLDLIRASETKMSPHDWTNLPKKSSWWMEERMAFVTMKTEGG 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 408
           SS+SS+PFLLE+LHL FLY  GLIL+ F YLRN  KSLK  RVGAS++
Sbjct: 361 SSISSQPFLLEKLHLFFLYFFGLILLVFGYLRNTIKSLKPTRVGASLT 402

BLAST of MC06g1379 vs. TAIR 10
Match: AT5G53050.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 523.5 bits (1347), Expect = 1.6e-148
Identity = 265/408 (64.95%), Postives = 314/408 (76.96%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPF EV     G E    N++I +FYRTYG G  K LLIIGLAGTH+SW PQI GL GTD
Sbjct: 1   MPFCEVVKEDVGPETTLNNAAIKIFYRTYGHGPIKALLIIGLAGTHESWGPQIMGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
            PNDD D D  GG             D  GIEVCAFDNRGMGRSSVPT KSEYTT IMA 
Sbjct: 61  KPNDDDDDD--GG----------IVSDDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMAN 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           D+I+LLDHLGW+KAH+ GHSMG MIACKLAAMAPERV SLA+LNVTGGGF+C PKLDR++
Sbjct: 121 DSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTGGGFECFPKLDRKS 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
             IA+RFLKAKTPEQRA+VDLDTHYSK+YLEE VG   RRA+LY++YVKGIS TGMQS Y
Sbjct: 181 LSIAIRFLKAKTPEQRAAVDLDTHYSKDYLEESVGTNTRRAILYQQYVKGISETGMQSKY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQ+ ACW+H +T+ EIE +RSAG LVSVIHGRHDVIAQI YAR+LA+RLYPVARMVD
Sbjct: 241 GFDGQINACWLHKITKVEIELIRSAGFLVSVIHGRHDVIAQICYARRLAQRLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVS ERT+EVN+ALL+LI+ASE K    DWTNL  ++  + + R++L+   TE  
Sbjct: 301 LHGGHLVSHERTEEVNKALLELIKASEMKKISTDWTNLTMETPGYFKRRLTLIRSSTEGK 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 409
           +  S   F+ E+ H   L+L GL+++AFEY R AF+++  V+VG S++
Sbjct: 361 NVASPAHFIAEKFHGFLLFLFGLLVLAFEYSRRAFRAVNPVKVGPSLT 396

BLAST of MC06g1379 vs. TAIR 10
Match: AT5G53050.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 523.5 bits (1347), Expect = 1.6e-148
Identity = 265/408 (64.95%), Postives = 314/408 (76.96%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPF EV     G E    N++I +FYRTYG G  K LLIIGLAGTH+SW PQI GL GTD
Sbjct: 1   MPFCEVVKEDVGPETTLNNAAIKIFYRTYGHGPIKALLIIGLAGTHESWGPQIMGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
            PNDD D D  GG             D  GIEVCAFDNRGMGRSSVPT KSEYTT IMA 
Sbjct: 61  KPNDDDDDD--GG----------IVSDDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMAN 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           D+I+LLDHLGW+KAH+ GHSMG MIACKLAAMAPERV SLA+LNVTGGGF+C PKLDR++
Sbjct: 121 DSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTGGGFECFPKLDRKS 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
             IA+RFLKAKTPEQRA+VDLDTHYSK+YLEE VG   RRA+LY++YVKGIS TGMQS Y
Sbjct: 181 LSIAIRFLKAKTPEQRAAVDLDTHYSKDYLEESVGTNTRRAILYQQYVKGISETGMQSKY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQ+ ACW+H +T+ EIE +RSAG LVSVIHGRHDVIAQI YAR+LA+RLYPVARMVD
Sbjct: 241 GFDGQINACWLHKITKVEIELIRSAGFLVSVIHGRHDVIAQICYARRLAQRLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEVNQALLDLIRASETKMNPHDWTNLPKKSSWWMEERMSLVTLKTEAG 360
           LHGGHLVS ERT+EVN+ALL+LI+ASE K    DWTNL  ++  + + R++L+   TE  
Sbjct: 301 LHGGHLVSHERTEEVNKALLELIKASEMKKISTDWTNLTMETPGYFKRRLTLIRSSTEGK 360

Query: 361 SSVSSKPFLLERLHLCFLYLLGLILMAFEYLRNAFKSLKSVRVGASIS 409
           +  S   F+ E+ H   L+L GL+++AFEY R AF+++  V+VG S++
Sbjct: 361 NVASPAHFIAEKFHGFLLFLFGLLVLAFEYSRRAFRAVNPVKVGPSLT 396

BLAST of MC06g1379 vs. TAIR 10
Match: AT5G53050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 452.2 bits (1162), Expect = 4.4e-127
Identity = 227/315 (72.06%), Postives = 253/315 (80.32%), Query Frame = 0

Query: 1   MPFIEVNSSTAGSENASINSSINLFYRTYGRGSTKVLLIIGLAGTHDSWCPQIKGLCGTD 60
           MPF EV     G E    N++I +FYRTYG G  K LLIIGLAGTH+SW PQI GL GTD
Sbjct: 1   MPFCEVVKEDVGPETTLNNAAIKIFYRTYGHGPIKALLIIGLAGTHESWGPQIMGLTGTD 60

Query: 61  VPNDDADRDVTGGRNAADASQESNSGDGGGIEVCAFDNRGMGRSSVPTKKSEYTTKIMAK 120
            PNDD D D  GG             D  GIEVCAFDNRGMGRSSVPT KSEYTT IMA 
Sbjct: 61  KPNDDDDDD--GG----------IVSDDSGIEVCAFDNRGMGRSSVPTHKSEYTTTIMAN 120

Query: 121 DAIALLDHLGWEKAHVFGHSMGGMIACKLAAMAPERVKSLAMLNVTGGGFQCCPKLDRQT 180
           D+I+LLDHLGW+KAH+ GHSMG MIACKLAAMAPERV SLA+LNVTGGGF+C PKLDR++
Sbjct: 121 DSISLLDHLGWKKAHIIGHSMGAMIACKLAAMAPERVLSLALLNVTGGGFECFPKLDRKS 180

Query: 181 FDIAVRFLKAKTPEQRASVDLDTHYSKEYLEEFVGFEKRRAVLYREYVKGISSTGMQSNY 240
             IA+RFLKAKTPEQRA+VDLDTHYSK+YLEE VG   RRA+LY++YVKGIS TGMQS Y
Sbjct: 181 LSIAIRFLKAKTPEQRAAVDLDTHYSKDYLEESVGTNTRRAILYQQYVKGISETGMQSKY 240

Query: 241 GFVGQVYACWMHNVTRKEIECLRSAGILVSVIHGRHDVIAQIFYARQLAERLYPVARMVD 300
           GF GQ+ ACW+H +T+ EIE +RSAG LVSVIHGRHDVIAQI YAR+LA+RLYPVARMVD
Sbjct: 241 GFDGQINACWLHKITKVEIELIRSAGFLVSVIHGRHDVIAQICYARRLAQRLYPVARMVD 300

Query: 301 LHGGHLVSRERTKEV 316
           LHGGHLVS ERT+EV
Sbjct: 301 LHGGHLVSHERTEEV 303

BLAST of MC06g1379 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 2.3e-06
Identity = 26/76 (34.21%), Postives = 38/76 (50.00%), Query Frame = 0

Query: 90  GIEVCAFDNRGMGRSSVPTKKSEYTTKIMAKDAIALLDHLGWEKAHVFGHSMGGMIACKL 149
           G    A D RG G S  P  +  YT   +  D + LLD LG ++  + GH  G ++A  L
Sbjct: 51  GYRAIAPDLRGYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWL 110

Query: 150 AAMAPERVKSLAMLNV 166
             + P+RV +L   +V
Sbjct: 111 CMIRPDRVNALVNTSV 126

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A8IAD84.6e-1231.40Putative aminoacrylate hydrolase RutD OS=Azorhizobium caulinodans (strain ATCC 4... [more]
B7KWT47.3e-1034.17Putative aminoacrylate hydrolase RutD OS=Methylorubrum extorquens (strain CM4 / ... [more]
B1M5I52.1e-0927.23Putative aminoacrylate hydrolase RutD OS=Methylobacterium radiotolerans (strain ... [more]
B1ZB182.8e-0933.33Putative aminoacrylate hydrolase RutD OS=Methylorubrum populi (strain ATCC BAA-7... [more]
P349133.6e-0940.00Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022158048.16.08e-298100.00uncharacterized protein LOC111024626 [Momordica charantia][more]
XP_038875890.15.92e-25184.80putative aminoacrylate hydrolase RutD [Benincasa hispida][more]
XP_023532415.14.24e-24883.62uncharacterized protein LOC111794595 [Cucurbita pepo subsp. pepo][more]
XP_022958692.12.45e-24783.62uncharacterized protein LOC111459843 [Cucurbita moschata][more]
XP_004145933.11.55e-24682.60uncharacterized protein LOC101203096 [Cucumis sativus] >KGN49859.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
A0A6J1DV102.94e-298100.00uncharacterized protein LOC111024626 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1H2I91.19e-24783.62uncharacterized protein LOC111459843 OS=Cucurbita moschata OX=3662 GN=LOC1114598... [more]
A0A0A0KLW77.49e-24782.60AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139... [more]
A0A6J1K3F37.97e-24682.64uncharacterized protein LOC111491334 OS=Cucurbita maxima OX=3661 GN=LOC111491334... [more]
A0A1S3AUJ92.50e-24582.35putative aminoacrylate hydrolase RutD OS=Cucumis melo OX=3656 GN=LOC103482970 PE... [more]
Match NameE-valueIdentityDescription
AT5G53050.21.6e-14864.95alpha/beta-Hydrolases superfamily protein [more]
AT5G53050.31.6e-14864.95alpha/beta-Hydrolases superfamily protein [more]
AT5G53050.14.4e-12772.06alpha/beta-Hydrolases superfamily protein [more]
AT4G02340.12.3e-0634.21alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 136..149
score: 30.52
coord: 90..105
score: 40.06
coord: 150..163
score: 36.69
coord: 300..322
score: 25.3
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 150..163
score: 35.71
coord: 90..105
score: 35.99
coord: 136..149
score: 40.89
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 83..165
e-value: 6.6E-21
score: 75.1
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 13..323
e-value: 2.3E-47
score: 164.1
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 21..321
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..90
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 5..405
NoneNo IPR availablePANTHERPTHR43329:SF1ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1379.1MC06g1379.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity