MC06g1372 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC06g1372
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionGlucan endo-1,3-beta-glucosidase
LocationMC06: 20898446 .. 20901305 (+)
RNA-Seq ExpressionMC06g1372
SyntenyMC06g1372
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTAAAATTATGCATCTCCACTAGCCACTATAATTTATTAAACCCATCGTCACCATACATTTGAACAATGGATTAATTAATTAATCCACTTGTTAGGTTCCTTCTCCACAATGACAAGAAAAGACAATCCCCTTTGCTTGGAGAAAAAGGCAAGATCCATTTTGGGAAATATTCTGTACAATAGCCTCACATATCAACCAATTAGTGAATTTCTAAGACTTAGTGTTCGAACTTACGACCTCTCTAAAGAACATAAATGACTAAATGAATACTAAATTGTTTAGAATTAAAGAATCACCTAGAACCAAAATGCTACAAGGTAGGTTACTAGAAGTGTGCTTCAACCTTCATCTAATAAATATATATATACATTTATCTATTTATTTATTTATTTTATGAAAAAGAAATAACACAGAACAAATTCAAAGGCAAAAAGGGGGGGGGGGAAAGACAAAAAAAGGTCTCTGAGAAGAACAAGCTTGAGCTGCATTACCAAGAACATACCCTCTCCCAATCCCAAAGCAAAGCATCTCAATGTTACTTCTTCAGCAATGGCCAATCCAATCCCACCCCAATTCACTCTTCTCAATCTCACTCTCCCATGGCTTCCCCTTTACCTCCCTCACGCCTTCTTGCTCTCACTTCAACTGCTGCCACTTTGCTTCTTCTTCTCCCCACTCTCATCCAAATCAAAGGTAATTATATATAAATGGCAGACAATTAAAAATATATATATATATGTGCATACTTCAATTATCGTGGTTGGTTTTTTACATTACCTCTGTTCAGGAGCGAAAGGAGGATCCATCGGAGTCAACTACGGCACGGTGGCCGACAATCTTCCTCCGCCGTCTAAGGTTGCACAATTCCTCTCCGATTCCACCATCATCAACCGCGTAAGACTCTTCGATGCCAATCCCGAAATCTTACGAGCGTTCGCTCACACCGGCATCTCATTAACCATCACCGTCCCCAACGATCAGATCCCTCGCTTGGCTAAACCTAATTTTGCAGAAGATTGGATCAAATTCAATGTCCAGCCCTACGTCCCTGCCACGGACATCATCCGCGTTCTAGTCGGAAACGAAGTCTTAGCCACCGCCAACAAATTGCTCATCGCCAACCTCGTCCCGGCGATGCAGAATCTCCACACAGCCCTCGCCGGAGCCTCCTTGGATCGCCGGATTCAAGTCTCGACGCCGCATTCTCTCGGAATCCTCTCCAACTCAAGCCCGCCGTCCACCGGAAGGTTCCGGCAAGGCTACGACACGCACGTTCTGAAGCCGATGCTCAACTTCCTGAGAGAAACAAATTCGGCGTTCATGGTGAATCCATACCCGTTCTTCGGCTCCTCCCCAGAAACCCTAGACTATGCGCTCTTCCGGCCAAATCCCGGCGTTTCCGATCCAGATTCTGGGCTTTTATATACCAACATGTTGGACGCTCAATTGGACGCTGTTTTTTCCGCCATGAAGAGTCTGGGCTTTGCGGATGTGGACATTGTAATTGCTGAAACGGGCTGGCCCTCCAAGGGCGACCCGGCCCAAGTCGGAATCGGCCCAAAAGAAGCCGCCGACTTCAACGGGAATCTCATGCGGCATGTCGTATCCGGCGTGGGCACGCCGCTGATGCCGAACCGGACCTTCGAGACTTACATTTTCGCTCTCTTCAACGAGGATCTGAAGCCCGGCCCAATATGTGAGAGAAACTTTGGGCTCTTTGAACCGGACTTGAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGTAGCTGCCACAGCCCGAACCCATCCTAAAACGTTTTTCATAATCATGATCGTTACATGTTGGTTGATTGGTTTGTTGTCGACTTGGATCTAACAGGCTCAGTCGTCTATTCCAAGAGCCCACCACGGCCCAGTTGAAGCCCCAGTACTGCCACCAAAAAGTCCAAGCCCAGTGACTGGAGGCAAAAAGTGGTGCTTGCCCAAGACTGGGGCCGACTCCGAGGCCTTGCAGAGAAACATTGACTATGTCTGTGGGATGGGCTTGGATTGTGGGCCGATCCAAGAAAATGGGCCATGTTTTGCTCCCAACACGGTCCGAGCTCATGCTGCTTACGTCATGAACGCGTACTTCCAAGCGACGGACGGAAACGATTACGATTGTGATTTTGATCAAACGGGAGCCCTAACCACCATGGATCCGAGTAAGTCGTTAATTTCTAATTTATACGATCAACATTTCATGTAAATATTAAATGGATAAAATTAATGGCAATAACTTAAATTCATTTACATAATTATAATTTTTTAAGTATAAAGATTAAATAGATGAACTATACATTTCTTTTAAAATTTACCTACTTTCTTCGGTTATTTCATTCTAAAATAAAATTGGGATGAATTGTAATTAATTAGAAGTGCTTAATTTGGTTTTATTTTTCAGGTTATGGGAAGTGCAAATATTGACAGAATGGAACATGGAAGAGGCCAATTACCTTTTATTATTACTCTTTTATTTGTTTGTTTTTTTTTTGTGTTATTTAATTTTTGTTTTTTTCTGTAGTTTCTTTAGTACAACATGTGGGGGTGGGGATTCGAACTTATGATCTTTTGGTCAGTATCTTAATCACTTGAACTATGCTTTTGACTTTTCTATGTTATTTAGCTGAAGAGTGTATGGGTAGAGAAGGAAAGATGGCCGGTTGAGTTCAACAAGGCCATTACGTATTTATAAATTGGAGGCATTTGCATTAATAGAATAATCTATATCTTTAGTACATGTAAAAGTCATTAGAAATTTTATGTGTCATTTTTGTCTTCTGATTATGATATTTTCAATATAAATTGGGAGGTAAAATTATGCAACTATTGTA

mRNA sequence

CTTTAAAATTATGCATCTCCACTAGCCACTATAATTTATTAAACCCATCGTCACCATACATTTGAACAATGGATTAATTAATTAATCCACTTGTTAGGTTCCTTCTCCACAATGACAAGAAAAGACAATCCCCTTTGCTTGGAGAAAAAGGCAAGATCCATTTTGGGAAATATTCTGTACAATAGCCTCACATATCAACCAATTAGTGAATTTCTAAGACTTAGTGTTCGAACTTACGACCTCTCTAAAGAACATAAATGACTAAATGAATACTAAATTGTTTAGAATTAAAGAATCACCTAGAACCAAAATGCTACAAGGTAGGTTACTAGAAGTGTGCTTCAACCTTCATCTAATAAATATATATATACATTTATCTATTTATTTATTTATTTTATGAAAAAGAAATAACACAGAACAAATTCAAAGGCAAAAAGGGGGGGGGGGAAAGACAAAAAAAGGTCTCTGAGAAGAACAAGCTTGAGCTGCATTACCAAGAACATACCCTCTCCCAATCCCAAAGCAAAGCATCTCAATGTTACTTCTTCAGCAATGGCCAATCCAATCCCACCCCAATTCACTCTTCTCAATCTCACTCTCCCATGGCTTCCCCTTTACCTCCCTCACGCCTTCTTGCTCTCACTTCAACTGCTGCCACTTTGCTTCTTCTTCTCCCCACTCTCATCCAAATCAAAGGAGCGAAAGGAGGATCCATCGGAGTCAACTACGGCACGGTGGCCGACAATCTTCCTCCGCCGTCTAAGGTTGCACAATTCCTCTCCGATTCCACCATCATCAACCGCGTAAGACTCTTCGATGCCAATCCCGAAATCTTACGAGCGTTCGCTCACACCGGCATCTCATTAACCATCACCGTCCCCAACGATCAGATCCCTCGCTTGGCTAAACCTAATTTTGCAGAAGATTGGATCAAATTCAATGTCCAGCCCTACGTCCCTGCCACGGACATCATCCGCGTTCTAGTCGGAAACGAAGTCTTAGCCACCGCCAACAAATTGCTCATCGCCAACCTCGTCCCGGCGATGCAGAATCTCCACACAGCCCTCGCCGGAGCCTCCTTGGATCGCCGGATTCAAGTCTCGACGCCGCATTCTCTCGGAATCCTCTCCAACTCAAGCCCGCCGTCCACCGGAAGGTTCCGGCAAGGCTACGACACGCACGTTCTGAAGCCGATGCTCAACTTCCTGAGAGAAACAAATTCGGCGTTCATGGTGAATCCATACCCGTTCTTCGGCTCCTCCCCAGAAACCCTAGACTATGCGCTCTTCCGGCCAAATCCCGGCGTTTCCGATCCAGATTCTGGGCTTTTATATACCAACATGTTGGACGCTCAATTGGACGCTGTTTTTTCCGCCATGAAGAGTCTGGGCTTTGCGGATGTGGACATTGTAATTGCTGAAACGGGCTGGCCCTCCAAGGGCGACCCGGCCCAAGTCGGAATCGGCCCAAAAGAAGCCGCCGACTTCAACGGGAATCTCATGCGGCATGTCGTATCCGGCGTGGGCACGCCGCTGATGCCGAACCGGACCTTCGAGACTTACATTTTCGCTCTCTTCAACGAGGATCTGAAGCCCGGCCCAATATGTGAGAGAAACTTTGGGCTCTTTGAACCGGACTTGAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGCTCAGTCGTCTATTCCAAGAGCCCACCACGGCCCAGTTGAAGCCCCAGTACTGCCACCAAAAAGTCCAAGCCCAGTGACTGGAGGCAAAAAGTGGTGCTTGCCCAAGACTGGGGCCGACTCCGAGGCCTTGCAGAGAAACATTGACTATGTCTGTGGGATGGGCTTGGATTGTGGGCCGATCCAAGAAAATGGGCCATGTTTTGCTCCCAACACGGTCCGAGCTCATGCTGCTTACGTCATGAACGCGTACTTCCAAGCGACGGACGGAAACGATTACGATTGTGATTTTGATCAAACGGGAGCCCTAACCACCATGGATCCGAGTTATGGGAAGTGCAAATATTGACAGAATGGAACATGGAAGAGGCCAATTACCTTTTATTATTACTCTTTTATTTGTTTGTTTTTTTTTTGTGTTATTTAATTTTTGTTTTTTTCTGTAGTTTCTTTAGTACAACATGTGGGGGTGGGGATTCGAACTTATGATCTTTTGGTCAGTATCTTAATCACTTGAACTATGCTTTTGACTTTTCTATGTTATTTAGCTGAAGAGTGTATGGGTAGAGAAGGAAAGATGGCCGGTTGAGTTCAACAAGGCCATTACGTATTTATAAATTGGAGGCATTTGCATTAATAGAATAATCTATATCTTTAGTACATGTAAAAGTCATTAGAAATTTTATGTGTCATTTTTGTCTTCTGATTATGATATTTTCAATATAAATTGGGAGGTAAAATTATGCAACTATTGTA

Coding sequence (CDS)

ATGGCTTCCCCTTTACCTCCCTCACGCCTTCTTGCTCTCACTTCAACTGCTGCCACTTTGCTTCTTCTTCTCCCCACTCTCATCCAAATCAAAGGAGCGAAAGGAGGATCCATCGGAGTCAACTACGGCACGGTGGCCGACAATCTTCCTCCGCCGTCTAAGGTTGCACAATTCCTCTCCGATTCCACCATCATCAACCGCGTAAGACTCTTCGATGCCAATCCCGAAATCTTACGAGCGTTCGCTCACACCGGCATCTCATTAACCATCACCGTCCCCAACGATCAGATCCCTCGCTTGGCTAAACCTAATTTTGCAGAAGATTGGATCAAATTCAATGTCCAGCCCTACGTCCCTGCCACGGACATCATCCGCGTTCTAGTCGGAAACGAAGTCTTAGCCACCGCCAACAAATTGCTCATCGCCAACCTCGTCCCGGCGATGCAGAATCTCCACACAGCCCTCGCCGGAGCCTCCTTGGATCGCCGGATTCAAGTCTCGACGCCGCATTCTCTCGGAATCCTCTCCAACTCAAGCCCGCCGTCCACCGGAAGGTTCCGGCAAGGCTACGACACGCACGTTCTGAAGCCGATGCTCAACTTCCTGAGAGAAACAAATTCGGCGTTCATGGTGAATCCATACCCGTTCTTCGGCTCCTCCCCAGAAACCCTAGACTATGCGCTCTTCCGGCCAAATCCCGGCGTTTCCGATCCAGATTCTGGGCTTTTATATACCAACATGTTGGACGCTCAATTGGACGCTGTTTTTTCCGCCATGAAGAGTCTGGGCTTTGCGGATGTGGACATTGTAATTGCTGAAACGGGCTGGCCCTCCAAGGGCGACCCGGCCCAAGTCGGAATCGGCCCAAAAGAAGCCGCCGACTTCAACGGGAATCTCATGCGGCATGTCGTATCCGGCGTGGGCACGCCGCTGATGCCGAACCGGACCTTCGAGACTTACATTTTCGCTCTCTTCAACGAGGATCTGAAGCCCGGCCCAATATGTGAGAGAAACTTTGGGCTCTTTGAACCGGACTTGAGCCCGGTTTATGAGATTGGGATTCTCCGGCCCACGGCTCAGTCGTCTATTCCAAGAGCCCACCACGGCCCAGTTGAAGCCCCAGTACTGCCACCAAAAAGTCCAAGCCCAGTGACTGGAGGCAAAAAGTGGTGCTTGCCCAAGACTGGGGCCGACTCCGAGGCCTTGCAGAGAAACATTGACTATGTCTGTGGGATGGGCTTGGATTGTGGGCCGATCCAAGAAAATGGGCCATGTTTTGCTCCCAACACGGTCCGAGCTCATGCTGCTTACGTCATGAACGCGTACTTCCAAGCGACGGACGGAAACGATTACGATTGTGATTTTGATCAAACGGGAGCCCTAACCACCATGGATCCGAGTTATGGGAAGTGCAAATATTGA

Protein sequence

MASPLPPSRLLALTSTAATLLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY
Homology
BLAST of MC06g1372 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 1.0e-132
Identity = 242/464 (52.16%), Postives = 315/464 (67.89%), Query Frame = 0

Query: 20  LLLLLPTLIQIKGAKGGS----IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANP 79
           L+LL+       GA+  +    IGVNYG  ADNLP P+ VA FL+  T I+RV+LFDANP
Sbjct: 4   LILLMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANP 63

Query: 80  EILRAFAHTGISLTITVPNDQIPRLAKP----NFAEDWIKFNVQPYVPATDIIRVLVGNE 139
             + AFA T ISL +++PN  +P LA      + A  WI+ N+ PYVPAT++  +L GNE
Sbjct: 64  TFISAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNE 123

Query: 140 VLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSP-PSTGRFRQGY 199
           +L + +  LI +L+PAM+ L  AL    L   ++V+TPH LGIL+ S   PS   FR GY
Sbjct: 124 ILLSTDTNLILSLLPAMRRLAQALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGY 183

Query: 200 DTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDA 259
           +T +   ML F R+T S FMVNPYP+F   PETL+YALFRPN G+ DP + L YT+MLDA
Sbjct: 184 NTKLFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDA 243

Query: 260 QLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTP 319
           Q+DA+++AMK LG+ DVDI + E GWP++ +P Q+G+G +EA DFN  ++R   SG GTP
Sbjct: 244 QMDAIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTP 303

Query: 320 LMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHHGP 379
           LMPNRTFETY+F+LF+E+ KPGPI ER+FGLF PD +PVY++G+LR  A S  P     P
Sbjct: 304 LMPNRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGA-SVAPTPSPNP 363

Query: 380 VEAPVLPPKSPSPV-TGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPN 439
                 P  SP P  +GG KWC+ K GA+   LQ NI+Y CG  +DC PIQ  G CF+PN
Sbjct: 364 -----SPNPSPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPN 423

Query: 440 TVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 474
           +++AHA+YVMNAY+QA    D  CDF  TG +T+ DPSYG CKY
Sbjct: 424 SLQAHASYVMNAYYQANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of MC06g1372 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 426.8 bits (1096), Expect = 3.2e-118
Identity = 215/441 (48.75%), Postives = 284/441 (64.40%), Query Frame = 0

Query: 38  IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQI 97
           IGVNYG VADNLPPPS+  + L  ST I +VRL+ A+P I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVKLL-QSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 98  PRLAK-PNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALA 157
           P LA  PN A  WI  NV P+ PA+ I+ + VGNE+L + +  L+  L+PAMQN+  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 158 GASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPF 217
             SL  +I+VST +S+ +L +S PPS+G F  GY T  LK +L FL +T S F +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 218 FG----SSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 277
           F       PETL + LF PN G  D  +G+ YTNM DAQ+DAV SA+KS+GF  V+IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 278 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 337
           ETGW S+GD  +VG     A  +NGNL+ H+ S VGTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 338 PICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCL 397
           P  ER FGLF+ DLS VY++G+ + ++ S                 ++PS       WC+
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSS-----------------QTPSGKVTSSGWCV 385

Query: 398 PKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYD 457
           PK GA +E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +     D
Sbjct: 386 PKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTD 445

Query: 458 CDFDQTGALTTMDPSYGKCKY 474
           CDF +T  +T+ +PSY  C Y
Sbjct: 446 CDFSKTATVTSQNPSYNNCVY 447

BLAST of MC06g1372 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 6.3e-114
Identity = 210/470 (44.68%), Postives = 293/470 (62.34%), Query Frame = 0

Query: 10  LLALTSTAATLLLLLPTLIQIKGAKGGS-IGVNYGTVADNLPPPSKVAQFLSDSTIINRV 69
           + A   T++ L L+   L     A   S +GVNYG ++DNLP        L  ST I +V
Sbjct: 1   MAANVQTSSLLFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLK-STTIQKV 60

Query: 70  RLFDANPEILRAFAHTGISLTITVPNDQIPRLA-KPNFAEDWIKFNVQPYVPATDIIRVL 129
           RLF A P +++AFA+TG+ + I   N  IP LA  PN A  ++K NV  + PA++II + 
Sbjct: 61  RLFGAEPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAIT 120

Query: 130 VGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFR 189
           VGNEVL + ++ LI+ L+PAMQN+  AL  ASL  +++VST H++ +LS S PPS+G F 
Sbjct: 121 VGNEVLTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFN 180

Query: 190 QGYDTHVLKPMLNFLRETNSAFMVNPYPFFG----SSPETLDYALFRPNPGVSDPDSGLL 249
            G     +K +L F    ++ FM++PYP+F      +P+TL + LF+PN G  D  +G  
Sbjct: 181 PGLG-DTMKALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHK 240

Query: 250 YTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHV 309
           YTNM DAQ+DAV SA+ ++GF D++IV+AETGWP  GD  +VG     A  + GNL+ H+
Sbjct: 241 YTNMFDAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHL 300

Query: 310 VSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSSI 369
            S VGTPLMP ++ +TY+F+L++ED K G   E+ FGLF+PD S  Y++G+L+ T   + 
Sbjct: 301 KSKVGTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQNPTT 360

Query: 370 PRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENG 429
           P               +P+P   G  WC+PK G   + L  NI+Y CG G+DCGPIQ  G
Sbjct: 361 PAT------------PTPTPKAAG-SWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGG 420

Query: 430 PCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 474
            CF PNTV+AHAAYVMN Y+Q+   N ++CDF QT  LT  +PSYG C +
Sbjct: 421 ACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNF 455

BLAST of MC06g1372 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 2.7e-93
Identity = 181/454 (39.87%), Postives = 266/454 (58.59%), Query Frame = 0

Query: 38  IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQI 97
           IG+ YG  ADNLP P++V++ +     I  VR++DAN ++L+AFA+TGI L I VPN  +
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQHLN-IKFVRIYDANIDVLKAFANTGIELMIGVPNADL 86

Query: 98  PRLAK-PNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALA 157
              A+  +  + W+  N+ PY P+T I  + VG EV    +      ++PAM+N+HTAL 
Sbjct: 87  LAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-ATGLVLPAMRNIHTALK 146

Query: 158 GASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPF 217
            + LD++I++S+ HSL ILS S PPS+  F + +    LKPML FL E  S FM++ YP+
Sbjct: 147 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSA-FLKPMLEFLVENESPFMIDLYPY 206

Query: 218 FGSSPET----LDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 277
           +     T    L+YALF  +  V DP +GLLY+NM DAQLDA++ A+ ++ F  V +++ 
Sbjct: 207 YAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 278 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 337
           E+GWPSKG P +    P+ A  +N NL+RHV+   GTP  P    + Y+F+LFNE+ KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 338 PICERNFGLFEPDLSPVYEIGI-------LRPTAQSSIPRAHHGPVEAPVLPPKSPSPVT 397
              ERN+G+F  + + VY +         + PT  +S       P  +P++   S   + 
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPT--NSTTGTSPSPSSSPIINGNSTVTIG 386

Query: 398 GG-----KKWCLPKTGADSEALQRNIDYVCGMG-LDCGPIQENGPCFAPNTVRAHAAYVM 457
           GG     KKWC+  + A    LQ  +D+ CG G +DC  +Q + PCF P+TV +HA+Y  
Sbjct: 387 GGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAF 446

Query: 458 NAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 474
           N Y+Q +  +  DC F+        DPSYG C Y
Sbjct: 447 NTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of MC06g1372 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.4e-89
Identity = 190/473 (40.17%), Postives = 263/473 (55.60%), Query Frame = 0

Query: 8   SRLLALTSTAATLLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINR 67
           +R   L  + + LLLLL          GG +GV YG  AD+LP PSKV Q +     I  
Sbjct: 2   ARDFKLIFSISILLLLLDC------CYGGKVGVCYGRSADDLPTPSKVVQLIQQHN-IKY 61

Query: 68  VRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAED-WIKFNVQPYVPATDIIRV 127
           VR++D N ++L+AF +T I L I VPN  +   ++     D W+K +V PY P T I  +
Sbjct: 62  VRIYDYNSQVLKAFGNTSIELMIGVPNSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYI 121

Query: 128 LVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRF 187
            VG E     +    + +VPAMQN+ TAL    L RRI+VST  SLGILS S PPS G F
Sbjct: 122 TVGAESTDDPHINASSFVVPAMQNVLTALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAF 181

Query: 188 RQGYDTHVLKPMLNFLRETNSAFMVNPYPFFG--SSPE--TLDYALFRPNPGVSDPDSGL 247
              Y  + L+PML FL E  S FM++ YP++    SP   +LDY LF  +  V DP++GL
Sbjct: 182 NSSY-AYFLRPMLEFLAENKSPFMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGL 241

Query: 248 LYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAAD-FNGNLMR 307
           LY NM DAQ+DA++ A+ +L F  + I++ ETGWP+KG P +      + A+ +N N++R
Sbjct: 242 LYKNMFDAQVDALYYALTALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIR 301

Query: 308 HVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQS 367
           HVV+  GTP  P      YIF+LFNE+ K G   ERN+GLF PD + VY++     T +S
Sbjct: 302 HVVTNQGTPAKPGEAMNVYIFSLFNENRKAGLDSERNWGLFYPDQTSVYQLDF---TGKS 361

Query: 368 SIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMG-LDCGPIQ 427
           +   ++     +                WC+  + A    L+  +D+ CG G +DC  IQ
Sbjct: 362 NGFHSNSSGTNSS----------GSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQ 421

Query: 428 ENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 474
            + PCF P+T+ +HA++V N+YFQ     D  C F   G     DPSY KC Y
Sbjct: 422 PSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGVKVNKDPSYDKCIY 453

BLAST of MC06g1372 vs. NCBI nr
Match: XP_038906871.1 (glucan endo-1,3-beta-glucosidase [Benincasa hispida])

HSP 1 Score: 833 bits (2151), Expect = 5.00e-302
Identity = 406/478 (84.94%), Postives = 442/478 (92.47%), Query Frame = 0

Query: 1   MASPLPPSRLLAL-TSTAATLLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQFL 60
           MASP  PS LL +  ST +T LLLLPTLIQI+GAK GSIGVNYGT+ADNLPPPSKVA FL
Sbjct: 30  MASPFTPSHLLFVHISTVSTFLLLLPTLIQIQGAKDGSIGVNYGTLADNLPPPSKVAAFL 89

Query: 61  SDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVP 120
            DSTII+RVRLFDANP+ILRAFAHTGIS++IT+PNDQIPRLAKPNFAE+WIKFN++PY+P
Sbjct: 90  LDSTIIDRVRLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIP 149

Query: 121 ATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSS 180
           AT+IIRVLVGNEVL+TANKLLIANLVPAMQ+LHTAL  ASLDRRIQ+STPHSLGILSNSS
Sbjct: 150 ATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILSNSS 209

Query: 181 PPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPD 240
           PPSTGRFRQGYDTHV+KPML+FLRETNS  MVNPYPFF  + E LDYALFRPNPGV D D
Sbjct: 210 PPSTGRFRQGYDTHVMKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVFDSD 269

Query: 241 SGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNL 300
           SG+LYTNMLDAQLDAV+SAMKSLGF+D+DIVIAETGWPSKGD AQ+G+GPKEAAD+NGNL
Sbjct: 270 SGILYTNMLDAQLDAVYSAMKSLGFSDLDIVIAETGWPSKGDQAQIGVGPKEAADYNGNL 329

Query: 301 MRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTA 360
           MRHVVSG GTPLMPNRTFETYIFALFNEDLKPGP+ ERNFGLFEPDLSPVYEIGILRPTA
Sbjct: 330 MRHVVSGKGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTA 389

Query: 361 QSSIPRAHHGPVEAPVLPPKSPSP----VTGGKKWCLPKTGADSEALQRNIDYVCGMGLD 420
           QSS P+AH GP+E  V  P+SPSP    V+ GK+WCLPKTGADSEALQRNIDYVCG+GLD
Sbjct: 390 QSSTPKAHQGPIEGSVSTPRSPSPSPSPVSEGKRWCLPKTGADSEALQRNIDYVCGLGLD 449

Query: 421 CGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           CGPIQENGPCFAPNTVRAHAAYVMNAYFQAT+GND DCDFDQTGALTT+DPSYGKCKY
Sbjct: 450 CGPIQENGPCFAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPSYGKCKY 507

BLAST of MC06g1372 vs. NCBI nr
Match: XP_023533272.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 819 bits (2115), Expect = 4.40e-297
Identity = 407/475 (85.68%), Postives = 436/475 (91.79%), Query Frame = 0

Query: 1   MASPLPPSRLL-ALTSTAATLLLLL-PTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQF 60
           MAS    SRLL A TS AATLLLLL PTL+ I+ AKGGSIGVNYGT+ADNLP PSKVA+F
Sbjct: 1   MASSFALSRLLRAYTSIAATLLLLLLPTLLHIQ-AKGGSIGVNYGTLADNLPLPSKVAEF 60

Query: 61  LSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYV 120
           L DSTII+R+RLFDANP+ILRAFAHT IS+TITVPNDQIPRL KPNFAE+WIKFNVQPYV
Sbjct: 61  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 120

Query: 121 PATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNS 180
           PATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LHTAL+ ASL RRIQVSTPHSLGILSNS
Sbjct: 121 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLSRRIQVSTPHSLGILSNS 180

Query: 181 SPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDP 240
           SPPST RFRQGYDTHV+KPML+FLRETNS  MVNPYPFFG++ ETLDYALFRPNPGV D 
Sbjct: 181 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 240

Query: 241 DSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGN 300
           DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIAETGWPSKGDPAQ+G+GPKEAAD+NGN
Sbjct: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 300

Query: 301 LMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGP+ ERNFGLFEPDLSPVYEIGILRPT
Sbjct: 301 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360

Query: 361 AQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGP 420
           AQSS PRAHHGP   P  P  SPSPV  GK WCLPKT ADSEALQRNIDYVCG+GLDCGP
Sbjct: 361 AQSSTPRAHHGPEPTPKGPSPSPSPVADGKTWCLPKTEADSEALQRNIDYVCGLGLDCGP 420

Query: 421 IQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           IQENGPCF PNTVRAHAAYVMNAYFQATDGND DCDF QTG LTT+DPSYGKCKY
Sbjct: 421 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKY 474

BLAST of MC06g1372 vs. NCBI nr
Match: XP_023521611.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 819 bits (2115), Expect = 7.73e-297
Identity = 407/475 (85.68%), Postives = 436/475 (91.79%), Query Frame = 0

Query: 1   MASPLPPSRLL-ALTSTAATLLLLL-PTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQF 60
           MAS    SRLL A TS AATLLLLL PTL+ I+ AKGGSIGVNYGT+ADNLP PSKVA+F
Sbjct: 16  MASSFALSRLLRAYTSIAATLLLLLLPTLLHIQ-AKGGSIGVNYGTLADNLPLPSKVAEF 75

Query: 61  LSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYV 120
           L DSTII+R+RLFDANP+ILRAFAHT IS+TITVPNDQIPRL KPNFAE+WIKFNVQPYV
Sbjct: 76  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 135

Query: 121 PATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNS 180
           PATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LHTAL+ ASL RRIQVSTPHSLGILSNS
Sbjct: 136 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLSRRIQVSTPHSLGILSNS 195

Query: 181 SPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDP 240
           SPPST RFRQGYDTHV+KPML+FLRETNS  MVNPYPFFG++ ETLDYALFRPNPGV D 
Sbjct: 196 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 255

Query: 241 DSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGN 300
           DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIAETGWPSKGDPAQ+G+GPKEAAD+NGN
Sbjct: 256 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 315

Query: 301 LMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGP+ ERNFGLFEPDLSPVYEIGILRPT
Sbjct: 316 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 375

Query: 361 AQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGP 420
           AQSS PRAHHGP   P  P  SPSPV  GK WCLPKT ADSEALQRNIDYVCG+GLDCGP
Sbjct: 376 AQSSTPRAHHGPEPTPKGPSPSPSPVADGKTWCLPKTEADSEALQRNIDYVCGLGLDCGP 435

Query: 421 IQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           IQENGPCF PNTVRAHAAYVMNAYFQATDGND DCDF QTG LTT+DPSYGKCKY
Sbjct: 436 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKY 489

BLAST of MC06g1372 vs. NCBI nr
Match: XP_022958170.1 (glucan endo-1,3-beta-glucosidase [Cucurbita moschata])

HSP 1 Score: 817 bits (2111), Expect = 1.66e-296
Identity = 407/475 (85.68%), Postives = 439/475 (92.42%), Query Frame = 0

Query: 1   MASPLPPSRLL-ALTSTAATLL-LLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQF 60
           MAS    SRLL A TS AATLL LLLPTL+Q++ AKGGSIGVNYGT+ADNLP PSKVA+F
Sbjct: 1   MASSFALSRLLRAYTSIAATLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVAEF 60

Query: 61  LSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYV 120
           L DSTII+R+RLFDANP+ILRAFAHT IS+TITVPNDQIPRL KPNFAE+WIKFNVQPYV
Sbjct: 61  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 120

Query: 121 PATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNS 180
           PATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LHTAL+ ASL+RRIQVSTPHSLGILSNS
Sbjct: 121 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILSNS 180

Query: 181 SPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDP 240
           SPPST RFRQGYDTHV+KPML+FLRETNS  MVNPYPFFG++ ETLDYALFRPNPGV D 
Sbjct: 181 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 240

Query: 241 DSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGN 300
           DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIAETGWPSKGDPAQ+G+GPKEAAD+NGN
Sbjct: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 300

Query: 301 LMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGP+ ERNFGLFEPDLSPVYEIGILRPT
Sbjct: 301 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360

Query: 361 AQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGP 420
           AQSS PRAHHGP   P  P  SPSPV  GK+WCLPKT ADSEALQRNIDYVCG+GLDCGP
Sbjct: 361 AQSSTPRAHHGPEPTPKGP--SPSPVADGKRWCLPKTEADSEALQRNIDYVCGLGLDCGP 420

Query: 421 IQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           IQENGPCF PNTVRAHAAYVMNAYFQATDGND DCDF QTG LTT+DPSYGKCKY
Sbjct: 421 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKY 472

BLAST of MC06g1372 vs. NCBI nr
Match: KAG7035758.1 (Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 816 bits (2107), Expect = 8.89e-295
Identity = 404/475 (85.05%), Postives = 436/475 (91.79%), Query Frame = 0

Query: 1   MASPLPPSRLL-ALTSTAATLLLLL-PTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQF 60
           MAS    SRLL A TS AATLLLLL PTL+Q++ AKGGSIGVNYGT+ADNLP PSKVA+F
Sbjct: 1   MASSFALSRLLRAYTSIAATLLLLLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVAEF 60

Query: 61  LSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYV 120
           L DSTII+R+RLFDANP+ILRAFAHT IS+TITVPNDQIPRL KPNFAE+WIKFNVQPYV
Sbjct: 61  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 120

Query: 121 PATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNS 180
           PATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LHTAL+ ASL+ RIQVSTPHSLGILSNS
Sbjct: 121 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNHRIQVSTPHSLGILSNS 180

Query: 181 SPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDP 240
           SPPST RFRQGYDTHV+KPML+FLRETNS  MVNPYPFFG++ ETLDYALFRPNPG  D 
Sbjct: 181 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGAYDS 240

Query: 241 DSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGN 300
           DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIAETGWPSKGDPAQ+G+GPKEAAD+NGN
Sbjct: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 300

Query: 301 LMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGP+ ERNFGLFEPDLSPVYEIGILRPT
Sbjct: 301 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360

Query: 361 AQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGP 420
           AQSS PRAHHGP   P  P   PSPV  GK+WCLPKT ADSEALQRNIDYVCG+GLDCGP
Sbjct: 361 AQSSTPRAHHGPEPTPKGPSPRPSPVADGKRWCLPKTEADSEALQRNIDYVCGLGLDCGP 420

Query: 421 IQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           IQENGPCF PNTVRAHAAYVMNAYFQATDGND DCDF QTG LTT+DPSYGKCKY
Sbjct: 421 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKY 474

BLAST of MC06g1372 vs. ExPASy TrEMBL
Match: A0A6J1H2P3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 PE=3 SV=1)

HSP 1 Score: 817 bits (2111), Expect = 8.04e-297
Identity = 407/475 (85.68%), Postives = 439/475 (92.42%), Query Frame = 0

Query: 1   MASPLPPSRLL-ALTSTAATLL-LLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQF 60
           MAS    SRLL A TS AATLL LLLPTL+Q++ AKGGSIGVNYGT+ADNLP PSKVA+F
Sbjct: 1   MASSFALSRLLRAYTSIAATLLILLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVAEF 60

Query: 61  LSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYV 120
           L DSTII+R+RLFDANP+ILRAFAHT IS+TITVPNDQIPRL KPNFAE+WIKFNVQPYV
Sbjct: 61  LLDSTIIDRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 120

Query: 121 PATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNS 180
           PATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LHTAL+ ASL+RRIQVSTPHSLGILSNS
Sbjct: 121 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNRRIQVSTPHSLGILSNS 180

Query: 181 SPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDP 240
           SPPST RFRQGYDTHV+KPML+FLRETNS  MVNPYPFFG++ ETLDYALFRPNPGV D 
Sbjct: 181 SPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 240

Query: 241 DSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGN 300
           DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIAETGWPSKGDPAQ+G+GPKEAAD+NGN
Sbjct: 241 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 300

Query: 301 LMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPGP+ ERNFGLFEPDLSPVYEIGILRPT
Sbjct: 301 LLRHVVSGVGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLSPVYEIGILRPT 360

Query: 361 AQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGP 420
           AQSS PRAHHGP   P  P  SPSPV  GK+WCLPKT ADSEALQRNIDYVCG+GLDCGP
Sbjct: 361 AQSSTPRAHHGPEPTPKGP--SPSPVADGKRWCLPKTEADSEALQRNIDYVCGLGLDCGP 420

Query: 421 IQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           IQENGPCF PNTVRAHAAYVMNAYFQATDGND DCDF QTG LTT+DPSYGKCKY
Sbjct: 421 IQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCDFQQTGDLTTVDPSYGKCKY 472

BLAST of MC06g1372 vs. ExPASy TrEMBL
Match: A0A6J1K780 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=3 SV=1)

HSP 1 Score: 806 bits (2083), Expect = 6.13e-292
Identity = 401/475 (84.42%), Postives = 433/475 (91.16%), Query Frame = 0

Query: 1   MASPLPPSRLL-ALTSTAATLLLLL-PTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQF 60
           MAS    SRLL A TS A TLLLLL PTL+Q++ AKGGSIGVNYGT+ADNLP PSKVA+F
Sbjct: 39  MASSFALSRLLRAYTSIAVTLLLLLLPTLLQMQ-AKGGSIGVNYGTLADNLPLPSKVAEF 98

Query: 61  LSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYV 120
           L DSTII R+RLFDANP+ILRAFAHT IS+TITVPNDQIPRL KPNFAE+WIKFNVQPYV
Sbjct: 99  LLDSTIIGRIRLFDANPDILRAFAHTRISVTITVPNDQIPRLVKPNFAEEWIKFNVQPYV 158

Query: 121 PATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNS 180
           PATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LHTAL+ ASL+ RIQVSTPHSLGILSNS
Sbjct: 159 PATDIIRILVGNEVLSTANKMLIANLVPAMQSLHTALSEASLNSRIQVSTPHSLGILSNS 218

Query: 181 SPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDP 240
           SPPST RFRQGYDTHV+KPML+FL+ETNS  MVNPYPFFG++ ETLDYALFRPNPGV D 
Sbjct: 219 SPPSTARFRQGYDTHVIKPMLSFLKETNSPLMVNPYPFFGTTAETLDYALFRPNPGVYDS 278

Query: 241 DSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGN 300
           DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIAETGWPSKGDPAQ+G+GPKEAAD+NGN
Sbjct: 279 DSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIAETGWPSKGDPAQIGVGPKEAADYNGN 338

Query: 301 LMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPT 360
           L+RHVVSGVGTPLMPNRTFETYIFALFNEDLKPGP+ ERNFGLFEPDL+PVYEIGILRPT
Sbjct: 339 LLRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLNPVYEIGILRPT 398

Query: 361 AQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGP 420
           AQSS PRAHHGP   P  P  SPSPV  GK+WCLPKT ADSEALQRNIDYVCG+G DCGP
Sbjct: 399 AQSSTPRAHHGPEPTPKGP--SPSPVADGKRWCLPKTEADSEALQRNIDYVCGLGFDCGP 458

Query: 421 IQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           IQ NGPCF PNTVRAHAAYVMNAYFQA DGND DCDF QTG LTT+DPSYGKCKY
Sbjct: 459 IQANGPCFLPNTVRAHAAYVMNAYFQAIDGNDSDCDFQQTGDLTTVDPSYGKCKY 510

BLAST of MC06g1372 vs. ExPASy TrEMBL
Match: A0A1S3AV36 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV=1)

HSP 1 Score: 791 bits (2043), Expect = 4.83e-286
Identity = 388/479 (81.00%), Postives = 431/479 (89.98%), Query Frame = 0

Query: 1   MASP-LPPSR--LLALTSTAAT-LLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVA 60
           MASP   PS   LL   ST  T LLL+LPTLIQI+G  GGSIGVNYGTVADNLPPPSKVA
Sbjct: 20  MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 79

Query: 61  QFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQP 120
            FL D+TII+RVRLFDA+P+ILRAFAHTGIS++IT+PNDQIPRL KPNFAE+WIKFN+QP
Sbjct: 80  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 139

Query: 121 YVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILS 180
           Y+PAT+IIRVLVGNEVL+TANKLLIANLVPAMQ+LHTAL  ASLDRRIQ+STPHSLGILS
Sbjct: 140 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 199

Query: 181 NSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVS 240
           NS+PPSTGRFRQGYDTHV+KPML+FLRETNS  MVNPYPFF  + E LDYALFRPNPGV 
Sbjct: 200 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 259

Query: 241 DPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFN 300
           D D G+LYTNMLDAQLDAV+SAMKS+ F D+DIVIAETGWPSKGDP+QVG+GPKEAA +N
Sbjct: 260 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 319

Query: 301 GNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVVSG GTPLMPNRTFETYIFALFNE+LKPGP+ ERNFGLFEPDL+PVYEIGILR
Sbjct: 320 GNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 379

Query: 361 PTAQSSIPRAHHGPVEAPVLPPKSPS--PVTGGKKWCLPKTGADSEALQRNIDYVCGMGL 420
           PTAQS+ PRAH GPVE P+  P+SPS   V+  K+WC+PKT A  EALQRNIDYVCG+GL
Sbjct: 380 PTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRNIDYVCGLGL 439

Query: 421 DCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           DCGPIQENGPC+APNTVR HAAYVMNAYFQAT+G ++DCDFDQTGALTT+DPSYGKC+Y
Sbjct: 440 DCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDPSYGKCRY 498

BLAST of MC06g1372 vs. ExPASy TrEMBL
Match: A0A0A0KJS0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1)

HSP 1 Score: 782 bits (2019), Expect = 1.74e-282
Identity = 382/465 (82.15%), Postives = 418/465 (89.89%), Query Frame = 0

Query: 10  LLALTSTAAT-LLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRV 69
           LL   ST  T LLL+LPTL QI+G  GGSIGVNYGTVADNLPPPSKVA FL D+TII+RV
Sbjct: 33  LLPYFSTLPTFLLLILPTLFQIQGVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRV 92

Query: 70  RLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLV 129
           RLFDA+P+ILRAFAHTGIS++IT+PNDQIPRL KPNFAE+WIKFN+QPY+PAT+IIRVLV
Sbjct: 93  RLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLV 152

Query: 130 GNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQ 189
           GNEVL+TANKLLIANLVPAMQ+LHTAL  ASLDRRIQ+STPHSLGILSNS+PPST RFRQ
Sbjct: 153 GNEVLSTANKLLIANLVPAMQSLHTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQ 212

Query: 190 GYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNML 249
           GYDTHV+KPML+FLRETNS  MVNPYPFF  + + LDYALFRPNPGV D D G+LYTNML
Sbjct: 213 GYDTHVIKPMLSFLRETNSPLMVNPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNML 272

Query: 250 DAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVG 309
           DAQLDAV+SAMKSLGF D+DIVIAETGWPSKGDP QVG+GPKEAA +NGNLMRHVVSG G
Sbjct: 273 DAQLDAVYSAMKSLGFEDLDIVIAETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKG 332

Query: 310 TPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHH 369
           TPLMPNRTFETYIFALFNE+LKPGPI ERNFGLFEPDLSPVYEIGILRPTAQS+ PRAH 
Sbjct: 333 TPLMPNRTFETYIFALFNENLKPGPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQ 392

Query: 370 GPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAP 429
           G VE P     SPS V+  K+WCLPK+ A  E LQRNIDYVCG+GLDCGPI+ENG CFAP
Sbjct: 393 GQVEGP-----SPSTVSESKRWCLPKSEASEEGLQRNIDYVCGLGLDCGPIKENGACFAP 452

Query: 430 NTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 473
           NTVRAHAAYVMNAYFQAT+GND+DCDFDQTG LTT+DPSYGKCKY
Sbjct: 453 NTVRAHAAYVMNAYFQATEGNDFDCDFDQTGTLTTVDPSYGKCKY 492

BLAST of MC06g1372 vs. ExPASy TrEMBL
Match: A0A5A7TGS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00250 PE=3 SV=1)

HSP 1 Score: 775 bits (2000), Expect = 8.30e-277
Identity = 381/472 (80.72%), Postives = 424/472 (89.83%), Query Frame = 0

Query: 1   MASP-LPPSR--LLALTSTAAT-LLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVA 60
           MASP   PS   LL   ST  T LLL+LPTLIQI+G  GGSIGVNYGTVADNLPPPSKVA
Sbjct: 1   MASPPFTPSHHLLLPYFSTLPTFLLLILPTLIQIQGVNGGSIGVNYGTVADNLPPPSKVA 60

Query: 61  QFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQP 120
            FL D+TII+RVRLFDA+P+ILRAFAHTGIS++IT+PNDQIPRL KPNFAE+WIKFN+QP
Sbjct: 61  AFLLDNTIIDRVRLFDADPDILRAFAHTGISVSITIPNDQIPRLVKPNFAEEWIKFNIQP 120

Query: 121 YVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILS 180
           Y+PAT+IIRVLVGNEVL+TANKLLIANLVPAMQ+LHTAL  ASLDRRIQ+STPHSLGILS
Sbjct: 121 YIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSLHTALVEASLDRRIQISTPHSLGILS 180

Query: 181 NSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVS 240
           NS+PPSTGRFRQGYDTHV+KPML+FLRETNS  MVNPYPFF  + E LDYALFRPNPGV 
Sbjct: 181 NSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVF 240

Query: 241 DPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFN 300
           D D G+LYTNMLDAQLDAV+SAMKS+ F D+DIVIAETGWPSKGDP+QVG+GPKEAA +N
Sbjct: 241 DSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVIAETGWPSKGDPSQVGVGPKEAAYYN 300

Query: 301 GNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILR 360
           GNLMRHVV+G GTPLMPNRTFETYIFALFNE+LKPGP+ ERNFGLFEPDL+PVYEIGILR
Sbjct: 301 GNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKPGPVGERNFGLFEPDLTPVYEIGILR 360

Query: 361 PTAQSSIPRAHHGPVEAPVLPPKSPS--PVTGGKKWCLPKTGADSEALQRNIDYVCGMGL 420
           PTAQS+ PRAH GPVE P+  P+SPS   V+  K+WC+PKT A  EALQRNIDYVCG+GL
Sbjct: 361 PTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKRWCVPKTEASVEALQRNIDYVCGLGL 420

Query: 421 DCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDP 466
           DCGPIQENGPC+APNTVR HAAYVMNAYFQAT+G ++DCDFDQTGALTT+DP
Sbjct: 421 DCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGKEFDCDFDQTGALTTVDP 472

BLAST of MC06g1372 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 594.7 bits (1532), Expect = 6.4e-170
Identity = 279/455 (61.32%), Postives = 357/455 (78.46%), Query Frame = 0

Query: 19  TLLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEIL 78
           ++  ++  L+ +      SIGVNYGT+A+NLPPP +VA+FL  ST+INR+RLFD +P+IL
Sbjct: 11  SMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQIL 70

Query: 79  RAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANK 138
           +AFA+TGI++T+TVPNDQIP L   + A+ WI  ++QP+ P+T+IIR+LVGNEV++TA+ 
Sbjct: 71  QAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADH 130

Query: 139 LLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPM 198
           LLI  L+PAMQ+LHTAL  ASL RRIQ+STPHSLG L+NS+PPS+ +FR+GYD  VLKP+
Sbjct: 131 LLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPL 190

Query: 199 LNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSA 258
           L+FLR T+S F+VNPYPFFG S ETLD+ALFRPNPG+ D  + LLYTNMLDAQLD+V+SA
Sbjct: 191 LSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSA 250

Query: 259 MKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFE 318
           M  LGF+DV+IVI E GWPS+GD  Q+G+    AA+FN NL+  V SG GTPLMPNRTFE
Sbjct: 251 MDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFE 310

Query: 319 TYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHHGPVEAPVLPP 378
           TYIFALFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT +SS           PV  P
Sbjct: 311 TYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSS----------DPVYNP 370

Query: 379 KSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYV 438
           +SP   +  K+WC+ K GA++ ALQRNIDYVCG+GLDC PI E G C+ PNTV+AH+ Y 
Sbjct: 371 RSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYA 430

Query: 439 MNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 474
           MN Y+Q  + +++DCDFD TG +TT+DPSYG C+Y
Sbjct: 431 MNLYYQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of MC06g1372 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 582.8 bits (1501), Expect = 2.5e-166
Identity = 275/449 (61.25%), Postives = 352/449 (78.40%), Query Frame = 0

Query: 19  TLLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEIL 78
           ++  ++  L+ +      SIGVNYGT+A+NLPPP +VA+FL  ST+INR+RLFD +P+IL
Sbjct: 11  SMFTIVGVLLILSTGSEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQIL 70

Query: 79  RAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANK 138
           +AFA+TGI++T+TVPNDQIP L   + A+ WI  ++QP+ P+T+IIR+LVGNEV++TA+ 
Sbjct: 71  QAFANTGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADH 130

Query: 139 LLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPM 198
           LLI  L+PAMQ+LHTAL  ASL RRIQ+STPHSLG L+NS+PPS+ +FR+GYD  VLKP+
Sbjct: 131 LLIRTLIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPL 190

Query: 199 LNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSA 258
           L+FLR T+S F+VNPYPFFG S ETLD+ALFRPNPG+ D  + LLYTNMLDAQLD+V+SA
Sbjct: 191 LSFLRSTSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSA 250

Query: 259 MKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFE 318
           M  LGF+DV+IVI E GWPS+GD  Q+G+    AA+FN NL+  V SG GTPLMPNRTFE
Sbjct: 251 MDKLGFSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFE 310

Query: 319 TYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHHGPVEAPVLPP 378
           TYIFALFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT +SS           PV  P
Sbjct: 311 TYIFALFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSS----------DPVYNP 370

Query: 379 KSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYV 438
           +SP   +  K+WC+ K GA++ ALQRNIDYVCG+GLDC PI E G C+ PNTV+AH+ Y 
Sbjct: 371 RSPVRGSSSKRWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYA 430

Query: 439 MNAYFQATDGNDYDCDFDQTGALTTMDPS 468
           MN Y+Q  + +++DCDFD TG +TT+DPS
Sbjct: 431 MNLYYQTMEKHEFDCDFDNTGEITTIDPS 449

BLAST of MC06g1372 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 426.8 bits (1096), Expect = 2.3e-119
Identity = 215/441 (48.75%), Postives = 284/441 (64.40%), Query Frame = 0

Query: 38  IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQI 97
           IGVNYG VADNLPPPS+  + L  ST I +VRL+ A+P I++A A TG+ + I   N  +
Sbjct: 26  IGVNYGQVADNLPPPSETVKLL-QSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDV 85

Query: 98  PRLAK-PNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALA 157
           P LA  PN A  WI  NV P+ PA+ I+ + VGNE+L + +  L+  L+PAMQN+  AL 
Sbjct: 86  PSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALE 145

Query: 158 GASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPF 217
             SL  +I+VST +S+ +L +S PPS+G F  GY T  LK +L FL +T S F +NPYPF
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLSDTGSPFAINPYPF 205

Query: 218 FG----SSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 277
           F       PETL + LF PN G  D  +G+ YTNM DAQ+DAV SA+KS+GF  V+IV+A
Sbjct: 206 FAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVA 265

Query: 278 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 337
           ETGW S+GD  +VG     A  +NGNL+ H+ S VGTPLMP +  +TYIFAL++E+LKPG
Sbjct: 266 ETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPG 325

Query: 338 PICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHHGPVEAPVLPPKSPSPVTGGKKWCL 397
           P  ER FGLF+ DLS VY++G+ + ++ S                 ++PS       WC+
Sbjct: 326 PSSERAFGLFKTDLSMVYDVGLAKSSSSS-----------------QTPSGKVTSSGWCV 385

Query: 398 PKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYD 457
           PK GA +E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +     D
Sbjct: 386 PKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTD 445

Query: 458 CDFDQTGALTTMDPSYGKCKY 474
           CDF +T  +T+ +PSY  C Y
Sbjct: 446 CDFSKTATVTSQNPSYNNCVY 447

BLAST of MC06g1372 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 426.4 bits (1095), Expect = 3.0e-119
Identity = 228/471 (48.41%), Postives = 305/471 (64.76%), Query Frame = 0

Query: 6   PPSRLLALTSTAATLLLLLPTLIQIKGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTII 65
           PPS  L L   AA + L +P +I        +IGVNYGT+  NLPPP++VA F+   T I
Sbjct: 5   PPSISLLLLLCAA-VFLTIPAVI-------SAIGVNYGTLG-NLPPPTQVANFIKTQTSI 64

Query: 66  NRVRLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIR 125
           + V++FD NP+ILRAFA TGIS+ +TVPN  IP LA    A  W+  N+ P+ P T I  
Sbjct: 65  DSVKIFDVNPDILRAFAGTGISVVVTVPNGDIPALANGRQARRWVSVNILPFHPQTKIKY 124

Query: 126 VLVGNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSN--SSPPST 185
           + VGNE+L T +  +I NL+PAM+NL+ AL  A + R ++V+T HSL I++   +  PS+
Sbjct: 125 ISVGNEILLTGDNNMINNLLPAMRNLNNALVRAGV-RDVKVTTAHSLNIIAYDLTGAPSS 184

Query: 186 GRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFR-PNPGVSDPDSGL 245
           GRFR G+D  +L P+L + R T S FMVNPYP+FG  P+ +++A+FR P   V DP +  
Sbjct: 185 GRFRPGWDKGILAPILAYHRRTKSPFMVNPYPYFGFDPKNVNFAIFRTPYKAVRDPFTRH 244

Query: 246 LYTNMLDAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRH 305
           +YTNM DA +D+ +SAMK+LG+ DV+IV+ ETGWPS  D       P  AA FN N+++ 
Sbjct: 245 VYTNMFDALMDSTYSAMKALGYGDVNIVVGETGWPSACDAP--WCSPANAAWFNLNIIKR 304

Query: 306 VVSGVGTPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSS 365
              G GTPLMPNR FETYIF LFNE+ KPGP  ERN+GLF  D SPVY++G+LR   Q  
Sbjct: 305 -AQGQGTPLMPNRRFETYIFGLFNEEGKPGPTAERNWGLFRADFSPVYDVGLLR-NGQGG 364

Query: 366 IPRAHHGPVEAPVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQEN 425
             R            P  P+P T G KWC+ ++GA +  LQ +I++VCG G+DC PIQ  
Sbjct: 365 GGR------------PALPAPSTAGGKWCVARSGATNTQLQDSINWVCGQGVDCKPIQAG 424

Query: 426 GPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 474
           G CF P+++R HA++VMNAYFQ+    D  C+F  TG +   +PS G CKY
Sbjct: 425 GSCFNPSSLRTHASFVMNAYFQSHGRTDGACNFSGTGMIVGNNPSNGACKY 449

BLAST of MC06g1372 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 426.0 bits (1094), Expect = 3.9e-119
Identity = 219/460 (47.61%), Postives = 292/460 (63.48%), Query Frame = 0

Query: 20  LLLLLPTLIQIKGAKGGS-IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEIL 79
           L LLL  L  I  +   S IGVNYG ++DNLPPPS+ A+ L  ST I +VRL++A+  I+
Sbjct: 6   LFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAKLL-QSTSIQKVRLYNADSSII 65

Query: 80  RAFAHTGISLTITVPNDQIPRLAKP-NFAEDWIKFNVQPYVPATDIIRVLVGNEVLATAN 139
            +   TGI + I V N  +P +A   N A  WI  NV P+ PA++II + VGNEVL + +
Sbjct: 66  TSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLSND 125

Query: 140 KLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKP 199
             L+  L+PAMQN+  AL   SL  +I+VST H++ +L NS PPS G F   Y    LK 
Sbjct: 126 LNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAG-LKG 185

Query: 200 MLNFLRETNSAFMVNPYPFFG----SSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLD 259
           +L FL +T S F +NPYPFF       PETL + LF+PNPG  D ++G+ Y NM DAQ+D
Sbjct: 186 ILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQVD 245

Query: 260 AVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMP 319
           AV SA+KS+GF  V++++AETGWPS GD  +VG   + A  +NGNL+ H+ S VGTPLMP
Sbjct: 246 AVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPLMP 305

Query: 320 NRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTAQSSIPRAHHGPVEA 379
            ++ +TYIFALF+E+LKPGP  E++FGLF+PDLS  Y+IG+ + T+  +      G V +
Sbjct: 306 GKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTKTTSSQTSQSPQLGKVTS 365

Query: 380 PVLPPKSPSPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRA 439
                           WC+PK  A  E LQ ++D+VCG G+DCGPI   G CF PN V +
Sbjct: 366 --------------MGWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFEPNNVAS 425

Query: 440 HAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPSYGKCKY 474
           H AY MN YFQ +  N  DCDF +T  +T+ +PSY  C Y
Sbjct: 426 HTAYAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVY 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524091.0e-13252.16Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q9M0693.2e-11848.75Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G866.3e-11444.68Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
Q8VYE52.7e-9339.87Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9FJU92.4e-8940.17Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Match NameE-valueIdentityDescription
XP_038906871.15.00e-30284.94glucan endo-1,3-beta-glucosidase [Benincasa hispida][more]
XP_023533272.14.40e-29785.68glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
XP_023521611.17.73e-29785.68glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo][more]
XP_022958170.11.66e-29685.68glucan endo-1,3-beta-glucosidase [Cucurbita moschata][more]
KAG7035758.18.89e-29585.05Glucan endo-1,3-beta-glucosidase, partial [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
A0A6J1H2P38.04e-29785.68(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 P... [more]
A0A6J1K7806.13e-29284.42(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=... [more]
A0A1S3AV364.83e-28681.00(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV... [more]
A0A0A0KJS01.74e-28282.15(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3... [more]
A0A5A7TGS68.30e-27780.72(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT5G24318.16.4e-17061.32O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.22.5e-16661.25O-Glycosyl hydrolases family 17 protein [more]
AT4G34480.12.3e-11948.75O-Glycosyl hydrolases family 17 protein [more]
AT3G55430.13.0e-11948.41O-Glycosyl hydrolases family 17 protein [more]
AT2G16230.13.9e-11947.61O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 389..473
e-value: 1.5E-41
score: 154.0
IPR012946X8 domainPFAMPF07983X8coord: 389..459
e-value: 4.6E-19
score: 68.9
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 38..352
e-value: 6.9E-77
score: 259.1
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 267..280
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 36..358
e-value: 3.7E-118
score: 396.1
NoneNo IPR availableGENE3D1.20.58.1040coord: 387..472
e-value: 1.5E-9
score: 40.0
NoneNo IPR availablePANTHERPTHR32227:SF62O-GLYCOSYL HYDROLASES FAMILY 17 PROTEINcoord: 27..466
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 27..466
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 38..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC06g1372.1MC06g1372.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds