MC06g1327 (gene) Bitter gourd (Dali-11) v1

BLAST of MC06g1327 vs. ExPASy Swiss-Prot
Match: Q43857 (Acid beta-fructofuranosidase OS=Vicia faba OX=3906 GN=VCINV PE=2 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 8.2e-256
Identity = 430/627 (68.58%), Postives = 504/627 (80.38%), Query Frame = 0

Query: 45  KEAVLIASGLLLVGLLI---AVIGQNGVVFYAKLEGSSSSEPPEMVCPA--SRGVSAGVS 104
           +E +L+ SGLLL+  +I   A I Q      A +  SSS    E   P   SRGVS+GVS
Sbjct: 21  RELLLLFSGLLLLASIIAFSAYIAQPHA--DADVSSSSSILSDEATRPTTLSRGVSSGVS 80

Query: 105 EKANRQFVGQNI----SPFPWNHSMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHF 164
           EK+N   +  N+      FPWN++ML WQRTAFHFQPE+NWMN   +  GPLY+KGWYHF
Sbjct: 81  EKSNTFLLSGNLVGEGGSFPWNNTMLSWQRTAFHFQPEKNWMN---DPNGPLYYKGWYHF 140

Query: 165 FYQYNPYDAVWGDISWGHAVSRDMIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMM 224
           FYQYNP  AVWGDI WGHAVSRD+IHWLHLPLA+VADQW+D +GVWTGSATILPDG+++M
Sbjct: 141 FYQYNPNGAVWGDIVWGHAVSRDLIHWLHLPLAMVADQWYDSNGVWTGSATILPDGQVIM 200

Query: 225 LYTGSTQESVQVQNLAYPANLSDPLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSE 284
           LYTGST E VQVQNLAYPA+L+DPLLVDWIKYP NPV++PPPGI  +DFRDPTTAWLT+E
Sbjct: 201 LYTGSTNEFVQVQNLAYPADLNDPLLVDWIKYPSNPVLVPPPGILPKDFRDPTTAWLTTE 260

Query: 285 GKWRFAIGSKINQTGISLMYETEDFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGL 344
           GKWR  IGSKIN+TG++L+Y+T DF+ ++  D LL AVPGTGMWECVDFFPVS   E GL
Sbjct: 261 GKWRITIGSKINKTGVALVYDTVDFKTYERKDMLLNAVPGTGMWECVDFFPVSMKSENGL 320

Query: 345 DTSVNGPAVKHVVKTSLDDDRHDYYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYAS 404
           DTS  G  VKHV+K SLDDDRHDYY+LGTYDEK   ++ DD   DVGIGLRYDYGI YAS
Sbjct: 321 DTSFTGDEVKHVMKVSLDDDRHDYYALGTYDEKKVKFIADDFENDVGIGLRYDYGIFYAS 380

Query: 405 KSFYDQHKRRRLLWGWIGESDSEVSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVE 464
           K+FYDQ K RR+LWGWIGESDSE +DV KGWASVQSIPR +  DKKTG+NLLQWPV EVE
Sbjct: 381 KTFYDQKKDRRVLWGWIGESDSEYADVAKGWASVQSIPRIVKLDKKTGSNLLQWPVAEVE 440

Query: 465 NLRQRNDTFNNLLVQAGSVVPLDVGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGG 524
           +LR R+D F NL V+ G+VV +D+  ATQLDI+AEFE+D+EA+ K   S++E+ C T GG
Sbjct: 441 SLRLRSDEFQNLKVKPGAVVSVDIETATQLDIVAEFEIDKEALEKTAQSNVEYECNTSGG 500

Query: 525 AAKRGALGPFGLLVLADESLTEHTPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIY 584
           A++RGALGPFGL VLAD  L+E+TP+YFYV KG NG L T FC+D+SRSS+ANDV K IY
Sbjct: 501 ASRRGALGPFGLYVLADNGLSEYTPVYFYVVKGINGKLHTSFCSDQSRSSLANDVHKQIY 560

Query: 585 GSSVPVLEDEKLSLRILVDHSVVESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTN 644
           GS VPVLE EKLSLRILVDHS+VESFAQGGR+CITSRVYPT+AIY  ARLF+FNNA +TN
Sbjct: 561 GSVVPVLEGEKLSLRILVDHSIVESFAQGGRTCITSRVYPTRAIYGAARLFLFNNAIETN 620

Query: 645 VTASITVWQMNSAFIRPYSPGEDSKKS 663
           VTAS+ VWQMNSAFIRPY P +  + S
Sbjct: 621 VTASLKVWQMNSAFIRPYHPDQKRQTS 642

BLAST of MC06g1327 vs. ExPASy Swiss-Prot
Match: P29001 (Acid beta-fructofuranosidase OS=Vigna radiata var. radiata OX=3916 GN=INVA PE=1 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 1.5e-252
Identity = 421/630 (66.83%), Postives = 501/630 (79.52%), Query Frame = 0

Query: 45  KEAVLIASGLLLVGLLIAVIGQNGV-VFYAKLEG----------SSSSEPPEMVCPASRG 104
           K+ + +  GLL +  L+A  G     V +A L            S +S P     P SRG
Sbjct: 22  KDLLFVLCGLLFLSSLVAYGGYRASGVPHAHLSSPTSNHQQDHQSPTSLPSSKWYPVSRG 81

Query: 105 VSAGVSEK-ANRQFVGQN--ISPFPWNHSMLMWQRTAFHFQPEENWMNGFENVTGPLYHK 164
           VS+GVSEK +N  F G+      FPW++SML WQRT+FHFQPE+NWMN   +  GP+Y+K
Sbjct: 82  VSSGVSEKSSNLLFAGEGGASEAFPWDNSMLSWQRTSFHFQPEKNWMN---DPNGPMYYK 141

Query: 165 GWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLHLPLAIVADQWFDISGVWTGSATILPD 224
           GWYHFFYQYNP  AVWGDI WGHAVSRDMIHWLHLPLA+VADQW+D  GVWTGSATILP+
Sbjct: 142 GWYHFFYQYNPNGAVWGDIVWGHAVSRDMIHWLHLPLAMVADQWYDKQGVWTGSATILPN 201

Query: 225 GRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDWIKYPGNPVILPPPGIDFRDFRDPTTA 284
           G I+MLYTGST ESVQVQNLAYPA+ SDPLL+DWIK+ GNPV++PPPGI  +DFRDPTTA
Sbjct: 202 GEIIMLYTGSTNESVQVQNLAYPADPSDPLLLDWIKHTGNPVLVPPPGIGAKDFRDPTTA 261

Query: 285 WLTSEGKWRFAIGSKINQTGISLMYETEDFENFQLLDNLLCAVPGTGMWECVDFFPVSKT 344
           WLTSEGKWR  IGSK+N+TGI+L+Y+TEDF+ ++L + LL AVPGTGMWECVDFFPVSK 
Sbjct: 262 WLTSEGKWRITIGSKLNKTGIALVYDTEDFKTYELKEGLLRAVPGTGMWECVDFFPVSKK 321

Query: 345 EEIGLDTSVNGPAVKHVVKTSLDDDRHDYYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYG 404
              GLDTSVNG  VKHV+K SLDDDRHDYY++GTYD+    + PDD   DVG+GLRYDYG
Sbjct: 322 NGNGLDTSVNGAEVKHVMKVSLDDDRHDYYAIGTYDDNKVLFTPDDVKNDVGVGLRYDYG 381

Query: 405 ILYASKSFYDQHKRRRLLWGWIGESDSEVSDVQKGWASVQSIPRTILFDKKTGTNLLQWP 464
           I YASK+FYDQ+K RR+LWGWIGESDSE +DV KGWASVQSIPRT+  D KTG+NLLQWP
Sbjct: 382 IFYASKTFYDQNKDRRILWGWIGESDSEYADVTKGWASVQSIPRTVRLDTKTGSNLLQWP 441

Query: 465 VEEVENLRQRNDTFNNLLVQAGSVVPLDVGKATQLDILAEFELDEEAVAKVVGSDIEFSC 524
           V+EVE+LR R+D F +L  + GSVV LD+  ATQLD++AEFE+D E++ K   S+ EF+C
Sbjct: 442 VDEVESLRLRSDEFKSLKAKPGSVVSLDIETATQLDVVAEFEIDTESLEKTAESNEEFTC 501

Query: 525 QTRGGAAKRGALGPFGLLVLADESLTEHTPIYFYVGKGQNGTLKTCFCADESRSSVANDV 584
            + GGAA+RGALGPFGLLVLADE L+E+TP+YFYV KG+NG L+T FC+D+SRSS ANDV
Sbjct: 502 SSSGGAAQRGALGPFGLLVLADEGLSEYTPVYFYVIKGRNGNLRTSFCSDQSRSSQANDV 561

Query: 585 FKPIYGSSVPVLEDEKLSLRILVDHSVVESFAQGGRSCITSRVYPTKAIYNDARLFVFNN 644
            K I+GS VPVL+ EK SLR+LVDHS+VESFAQGGR+C+TSRVYPTKAIY  ARLF+FNN
Sbjct: 562 RKQIFGSVVPVLKGEKFSLRMLVDHSIVESFAQGGRTCVTSRVYPTKAIYGAARLFLFNN 621

Query: 645 AKDTNVTASITVWQMNSAFIRPYSPGEDSK 661
           A +  VTAS+ VWQMNSAFIRP+    D K
Sbjct: 622 ATEATVTASLKVWQMNSAFIRPFPFNPDQK 648

BLAST of MC06g1327 vs. ExPASy Swiss-Prot
Match: O24509 (Acid beta-fructofuranosidase OS=Phaseolus vulgaris OX=3885 PE=2 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 6.7e-250
Identity = 419/636 (65.88%), Postives = 501/636 (78.77%), Query Frame = 0

Query: 42  RPIKEAVLIASGLLLVGLLIAVIGQNGV--VFYAKLEGSSSSEPPEMVCP---------- 101
           R  K+ +L+   LL +  L+A  G+N    V +  +  S+S+   E   P          
Sbjct: 19  RTRKDLLLLLCALLFLSSLVA-FGRNRASNVPHDHVSSSASNHQQEHQSPTSLPSSKWHA 78

Query: 102 ASRGVSAGVSEKANRQFV---GQNISPFPWNHSMLMWQRTAFHFQPEENWMNGFENVTGP 161
            SRGVS+GVSEK++       G     FPW++SML WQRT+FHFQPE+NWMN   +  GP
Sbjct: 79  VSRGVSSGVSEKSSSMLFSGEGGASEAFPWDNSMLSWQRTSFHFQPEKNWMN---DPNGP 138

Query: 162 LYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLHLPLAIVADQWFDISGVWTGSAT 221
           +Y+KGWYHFFYQYNP  AVWGDI WGHAVSRDMIHWLHLPLA+VADQW+D  GVWTGSAT
Sbjct: 139 MYYKGWYHFFYQYNPNGAVWGDIVWGHAVSRDMIHWLHLPLAMVADQWYDKQGVWTGSAT 198

Query: 222 ILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDWIKYPGNPVILPPPGIDFRDFRD 281
           ILP+G I+MLYTGST ESVQVQNLAYPA+ SDPLLVDWIK+PGNPV++PPPGI  +DFRD
Sbjct: 199 ILPNGEIIMLYTGSTNESVQVQNLAYPADPSDPLLVDWIKHPGNPVLVPPPGIGAKDFRD 258

Query: 282 PTTAWLTSEGKWRFAIGSKINQTGISLMYETEDFENFQLLDNLLCAVPGTGMWECVDFFP 341
           PTTAWLTSEGKWR  IGSK+N+TGI+L+Y+T+DF+ ++L +  L AVPGTGMWECVDFFP
Sbjct: 259 PTTAWLTSEGKWRITIGSKLNKTGIALVYDTDDFKTYELKNGHLRAVPGTGMWECVDFFP 318

Query: 342 VSKTEEIGLDT--SVNGPAVKHVVKTSLDDDRHDYYSLGTYDEKTGTWVPDDPTIDVGIG 401
           VSK  E GLDT  S+NG  VK+V+K SLDDDRHDYY++GTYDE    + PDD   DVG+G
Sbjct: 319 VSKKNENGLDTSLSINGAEVKYVMKVSLDDDRHDYYTIGTYDENKVLFTPDDVKNDVGVG 378

Query: 402 LRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVSDVQKGWASVQSIPRTILFDKKTGT 461
           LRYDYGI YASK+FYDQ+  RR+LWGWIGESDSE +DV KGWASVQSIPRT+  DKKTG+
Sbjct: 379 LRYDYGIFYASKTFYDQNMDRRILWGWIGESDSEYADVTKGWASVQSIPRTVRLDKKTGS 438

Query: 462 NLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVGKATQLDILAEFELDEEAVAKVVGS 521
           NLLQWPV EVE+LR R+D F +L  + GSVV LD+  ATQLD++AEFE+D E++ K   S
Sbjct: 439 NLLQWPVAEVESLRLRSDEFKSLKAKPGSVVSLDIETATQLDVVAEFEIDAESLQKTAQS 498

Query: 522 DIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTPIYFYVGKGQNGTLKTCFCADESRS 581
           + EF+C T GGAA+RGALGPFGLLVLADE L+E+TP+YFYV KG+NG LKT FC+D+SRS
Sbjct: 499 NEEFTCSTSGGAAQRGALGPFGLLVLADEGLSEYTPVYFYVIKGRNGNLKTSFCSDQSRS 558

Query: 582 SVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVESFAQGGRSCITSRVYPTKAIYNDAR 641
           S  NDV K I+G+ VPVLE EK SLR+LVDHS+VESFAQGGR+C+TSRVYPTKAIY  AR
Sbjct: 559 SQPNDVRKQIFGNIVPVLEGEKFSLRMLVDHSIVESFAQGGRTCVTSRVYPTKAIYGAAR 618

Query: 642 LFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSK 661
           LF+FNNA +  VTAS+ +WQMNSAFIRP+    D K
Sbjct: 619 LFLFNNATEATVTASLKIWQMNSAFIRPFPFNPDQK 650

BLAST of MC06g1327 vs. ExPASy Swiss-Prot
Match: H2DF88 (Acid beta-fructofuranosidase 2, vacuolar OS=Rosa hybrid cultivar OX=128735 PE=2 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 7.0e-247
Identity = 415/659 (62.97%), Postives = 511/659 (77.54%), Query Frame = 0

Query: 23  YSPLPDEPAQTVDSPVIRRRPIKE-AVLIASGLLLVGLLIAVIGQNGVVFYAKLEGSSSS 82
           Y+PLP +P  +   P   RRP+K  AV+ AS + L+ L+  +I Q               
Sbjct: 8   YTPLPGDPFLS-GPPETPRRPLKGFAVIFASVIFLMSLVALIIHQ------------GPQ 67

Query: 83  EPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSMLMWQRTAFHFQPEENWMNGFE 142
           +PP+++               ++Q   ++  P    ++ML WQRTAFHFQPE+NWMN   
Sbjct: 68  QPPDVM--------------PDKQ--DEHHHPQSTTNTMLSWQRTAFHFQPEKNWMN--- 127

Query: 143 NVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLHLPLAIVADQWFDISGVW 202
           +  GP+Y+KGWYHFFYQYNP  AVWG+I WGHAVSRD+IHWLHLPLA+VADQW+DI+GVW
Sbjct: 128 DPNGPMYYKGWYHFFYQYNPRGAVWGNIVWGHAVSRDLIHWLHLPLAMVADQWYDINGVW 187

Query: 203 TGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDWIKYPGNPVILPPPGIDF 262
           TGSATILP+ +I+MLYTGST ESVQVQ LAYPA+  DPLL  W+KY GNPV++PPPGI  
Sbjct: 188 TGSATILPNDQIVMLYTGSTNESVQVQCLAYPADHKDPLLTKWVKYSGNPVLVPPPGIGV 247

Query: 263 RDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDFENFQLLDNLLCAVPGTGMWEC 322
           +DFRDPTTAW  +EGKWR  IGSK+N+TGISL+Y+T+DF  ++ LD +L AVPGTGMWEC
Sbjct: 248 KDFRDPTTAWYITEGKWRITIGSKVNKTGISLVYDTKDFIKYEQLDGVLHAVPGTGMWEC 307

Query: 323 VDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYYSLGTYDEKTGTWVPDDPTIDV 382
           +DF+PVSKT + GLDTS NG  VKHV+K SLDDDR+DYY+LG+Y+EKTG WVPD+  IDV
Sbjct: 308 IDFYPVSKTSDKGLDTSQNGADVKHVMKASLDDDRNDYYALGSYNEKTGKWVPDNQKIDV 367

Query: 383 GIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVSDVQKGWASVQSIPRTILFDKK 442
           GIG+RYDYG  YASK+FYDQ+K+RR+LWGWIGESDSE +DV+KGWAS+Q IPRT+LFD+K
Sbjct: 368 GIGIRYDYGKFYASKTFYDQNKQRRVLWGWIGESDSENADVKKGWASLQGIPRTVLFDQK 427

Query: 443 TGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVGKATQLDILAEFELDEEAVAKV 502
           TG+NLLQWPVEE+E LR     F+ + V+AGSVVPLDVG ATQLDI+AEF+LD++ V   
Sbjct: 428 TGSNLLQWPVEEIEKLRLNKKNFDKVQVKAGSVVPLDVGTATQLDIVAEFQLDQKVVESA 487

Query: 503 VGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTPIYFYVGKGQNGTLKTCFCADE 562
             ++ EFSCQT GGAAKRGALGPFGLLVLAD+SL+E TP+YFYV KG  GT+ T FCAD+
Sbjct: 488 AETNEEFSCQTSGGAAKRGALGPFGLLVLADDSLSERTPVYFYVVKGSGGTVNTYFCADQ 547

Query: 563 SRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVESFAQGGRSCITSRVYPTKAIYN 622
           +RSSVA DV K + GS VPVL+ E LSLRILVDHS++ESFAQGGR+ IT+RVYPT+AIY 
Sbjct: 548 TRSSVATDVVKQVSGSYVPVLKGETLSLRILVDHSIIESFAQGGRTTITTRVYPTQAIYG 607

Query: 623 DARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSKKSQSSVV-----LVPFYLFL 676
            ARLF+FNNA DT+ TAS+ +WQMNSAFIRP+SP   S  S + V      +VPF +FL
Sbjct: 608 AARLFLFNNATDTSFTASLQIWQMNSAFIRPFSPDAASHSSFTPVTVFIKFIVPFGIFL 634

BLAST of MC06g1327 vs. ExPASy Swiss-Prot
Match: P80065 (Beta-fructofuranosidase, soluble isoenzyme I OS=Daucus carota OX=4039 GN=INV*DC4 PE=1 SV=2)

HSP 1 Score: 836.3 bits (2159), Expect = 2.6e-241
Identity = 399/644 (61.96%), Postives = 492/644 (76.40%), Query Frame = 0

Query: 23  YSPLPDEP----AQTVDSPVIRRRPIK--EAVLIASGLLLVGLLIAVIGQNGVVFYAKLE 82
           Y+P PD P        D     RRPIK   +VL+++ +L   + + V      V   K+ 
Sbjct: 18  YTPRPDSPETRHEPDPDRSKTNRRPIKIVSSVLLSTLILSFVIFLLVNPNVQQVVRKKVS 77

Query: 83  GSS-------SSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSMLMWQRTAFH 142
            +S       +S+ PEM+ P SRGVS GVSEK+ RQ   +    +PW + ML WQRT+FH
Sbjct: 78  KNSNGEDRNKASKSPEMLGPPSRGVSQGVSEKSFRQATAE--PSYPWTNDMLSWQRTSFH 137

Query: 143 FQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLHLPLAI 202
           FQP+ENWMN   +  GPL+H GWYH FYQYNP  A+WG+I+WGHA+SRD+I+WLHLP A+
Sbjct: 138 FQPQENWMN---DPNGPLFHMGWYHLFYQYNPDSAIWGNITWGHAISRDLINWLHLPFAM 197

Query: 203 VADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDWIKYPG 262
             DQW+DI+GVWTGSAT+LPDG+I+MLYTG T + VQVQNLAYPANLSDPLL+DWIKYP 
Sbjct: 198 QPDQWYDINGVWTGSATVLPDGKIVMLYTGDTDDLVQVQNLAYPANLSDPLLLDWIKYPD 257

Query: 263 NPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDFENFQLLDNL 322
           NPV+ PPPGI   DFRDPTTAW+  +GKWR  IGSK+N+TGISLMY+T DF  ++LLDNL
Sbjct: 258 NPVMFPPPGIGSTDFRDPTTAWIGRDGKWRITIGSKVNKTGISLMYKTTDFITYELLDNL 317

Query: 323 LCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYYSLGTYDEKT 382
           L AVPGTGMWECVDF+PVS T   GLDTSVNGP VKHV+K+SLDDDRHDYY+LGTYD   
Sbjct: 318 LHAVPGTGMWECVDFYPVSVTGSNGLDTSVNGPGVKHVLKSSLDDDRHDYYALGTYDPIN 377

Query: 383 GTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVSDVQKGWASV 442
             W PD+P +DVGIGLR DYG  YASK+FYDQ K RRLLWGWIGE+DSE +D+ KGWASV
Sbjct: 378 DKWTPDNPELDVGIGLRLDYGKYYASKTFYDQDKERRLLWGWIGETDSESADLLKGWASV 437

Query: 443 QSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVGKATQLDILA 502
           QSIPRT++FDKKTGTN+LQWPV+EVE+LR R+   +++ ++ GS+VPL +  A QLDI+A
Sbjct: 438 QSIPRTVVFDKKTGTNILQWPVKEVESLRSRSYEIDDVELKPGSLVPLKISSAAQLDIVA 497

Query: 503 EFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTPIYFYVGKGQ 562
            FE+DEEA      +D  ++C    GAA RG LGPFG+LVLAD+ L+E TP+YFY+ KG 
Sbjct: 498 SFEVDEEAFKGTYEADASYNCTASEGAAGRGILGPFGILVLADDPLSELTPVYFYIAKGV 557

Query: 563 NGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVESFAQGGRSCI 622
           +G  KT FCAD+SRSS A+DV K +YGS VPVL  E LS+R+LVDHS+VESFAQGGR+ I
Sbjct: 558 DGNAKTYFCADQSRSSTASDVDKEVYGSDVPVLHGESLSMRLLVDHSIVESFAQGGRTVI 617

Query: 623 TSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPY 654
           TSRVYPT+AIY+ AR+F+FNNA   +VTAS+  WQM SA ++P+
Sbjct: 618 TSRVYPTRAIYSAARVFLFNNATGVSVTASVKAWQMASATLKPF 656

BLAST of MC06g1327 vs. NCBI nr
Match: XP_022156446.1 (acid beta-fructofuranosidase-like [Momordica charantia])

HSP 1 Score: 1394 bits (3608), Expect = 0.0
Identity = 682/689 (98.98%), Postives = 683/689 (99.13%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL
Sbjct: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60

Query: 61  IAVIGQNGVVFYAKLEGSSSSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSM 120
           IAVIGQNGVVFYAKLEGSSSSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSM
Sbjct: 61  IAVIGQNGVVFYAKLEGSSSSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSM 120

Query: 121 LMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMI 180
           LMWQRTAFHFQPEENWMN   +  GPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMI
Sbjct: 121 LMWQRTAFHFQPEENWMN---DPNGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMI 180

Query: 181 HWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPL 240
           HWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPL
Sbjct: 181 HWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPL 240

Query: 241 LVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDF 300
           LVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDF
Sbjct: 241 LVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDF 300

Query: 301 ENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYY 360
           ENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYY
Sbjct: 301 ENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYY 360

Query: 361 SLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVS 420
           SLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVS
Sbjct: 361 SLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVS 420

Query: 421 DVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVG 480
           DVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVG
Sbjct: 421 DVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVG 480

Query: 481 KATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTP 540
           KATQLDILAEFELDEEAVAKVV SDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTP
Sbjct: 481 KATQLDILAEFELDEEAVAKVVXSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTP 540

Query: 541 IYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVES 600
           IYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVES
Sbjct: 541 IYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVES 600

Query: 601 FAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSK 660
           FAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSK
Sbjct: 601 FAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSK 660

Query: 661 KSQSSVVLVPFYLFLLVSLLHLITWDVQG 689
           KSQSSVVLVPFYLFLLVSLLHLITWDVQG
Sbjct: 661 KSQSSVVLVPFYLFLLVSLLHLITWDVQG 686

BLAST of MC06g1327 vs. NCBI nr
Match: XP_038906769.1 (acid beta-fructofuranosidase-like [Benincasa hispida])

HSP 1 Score: 1149 bits (2973), Expect = 0.0
Identity = 558/691 (80.75%), Postives = 614/691 (88.86%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MVN NPFSAFF SGD P +LPVY+PLPDEP   V+SP   RRPIK AVLI+SGLLLV LL
Sbjct: 1   MVNFNPFSAFFRSGDRPENLPVYNPLPDEP---VESPATHRRPIKLAVLISSGLLLVCLL 60

Query: 61  IAVIGQNGVVFYAKLEGSS--SSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+IGQNGV + A L G S   S+ PE+   ASRGVSAGVSEKANRQF+GQN++PFPWN+
Sbjct: 61  VAIIGQNGVGYLATLRGLSFLKSKSPEIFRTASRGVSAGVSEKANRQFLGQNVAPFPWNN 120

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPE NWMN   +  GPLY+KGWYHFFYQ+NP+ AVWG+I WGHAVSRD
Sbjct: 121 SMLSWQRTAFHFQPEGNWMN---DPNGPLYYKGWYHFFYQHNPHAAVWGNIVWGHAVSRD 180

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHWLHLPLA+V DQW+DI+GVWTGSATILPDG+IMMLYTGST+E VQVQNLAYPANLSD
Sbjct: 181 LIHWLHLPLALVPDQWYDINGVWTGSATILPDGQIMMLYTGSTKEHVQVQNLAYPANLSD 240

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL+DW+K+PGNPV+ PPPGI FRDFRDPTTAWLTS+GKWR AIGSK+N+TGISL+YETE
Sbjct: 241 PLLIDWVKFPGNPVLFPPPGIHFRDFRDPTTAWLTSKGKWRLAIGSKVNRTGISLLYETE 300

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF++FQLLDNLLCAV GTGMWEC+DFFPVSK  +IGLDTSVNGP VKHVVKTSLDDDRHD
Sbjct: 301 DFKSFQLLDNLLCAVAGTGMWECLDFFPVSKAGKIGLDTSVNGPEVKHVVKTSLDDDRHD 360

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKT TWVPDDP I+VGIGLRYDYGI YASKSFYD  K RR+LWGWIGESDSE
Sbjct: 361 YYSLGTYDEKTCTWVPDDPKINVGIGLRYDYGIFYASKSFYDHKKGRRVLWGWIGESDSE 420

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVE+LRQR+  FNNL+VQ GSVVPLD
Sbjct: 421 FADVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVESLRQRSLAFNNLVVQPGSVVPLD 480

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           VG +TQLDI AEFELDEEAVAK + S+ EFSCQ+RGGAA RGALGPFGL VLADESL+EH
Sbjct: 481 VGTSTQLDIFAEFELDEEAVAKAIESNEEFSCQSRGGAADRGALGPFGLSVLADESLSEH 540

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV KGQNGTLKT FC DESRSS ANDV KPIYGSSVPVLE EKLSLRILVDHSVV
Sbjct: 541 TPVYFYVAKGQNGTLKTFFCTDESRSSEANDVIKPIYGSSVPVLEGEKLSLRILVDHSVV 600

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CI+SR+YPTKAIY  ARLFVFNNAKDTNVTAS T+WQMN AFIRPY P ED
Sbjct: 601 ESFAQGGRTCISSRIYPTKAIYGAARLFVFNNAKDTNVTASFTIWQMNPAFIRPYRPSED 660

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLITWDVQG 689
           +  S+SSVVL+P Y+FL VSLLHLI WDV G
Sbjct: 661 NDSSRSSVVLLPLYMFLFVSLLHLIIWDVLG 685

BLAST of MC06g1327 vs. NCBI nr
Match: XP_004147588.1 (acid beta-fructofuranosidase [Cucumis sativus] >KGN50220.1 hypothetical protein Csa_000255 [Cucumis sativus])

HSP 1 Score: 1108 bits (2865), Expect = 0.0
Identity = 531/690 (76.96%), Postives = 597/690 (86.52%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MV  N F  FF SG  P +LP YSPLP +P Q   SP I+RRP+K  +LI+SG  LV LL
Sbjct: 1   MVTFNHFPTFFASGHHPENLPFYSPLPGDPLQ---SPSIQRRPVKRTLLISSGFFLVCLL 60

Query: 61  IAVIGQNGVVFYAKLEGSS--SSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+I QN V F A   G S  SS+ PE++ P SRGVS GVSEKANR F+GQN++ FPWN+
Sbjct: 61  VAIIVQNNVDFVATFPGLSFLSSKSPEILPPVSRGVSEGVSEKANRHFIGQNLAYFPWNN 120

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPEENWMN   +  GPLY+ GWYHFFYQYNP  AVWG+I WGHAVS D
Sbjct: 121 SMLSWQRTAFHFQPEENWMN---DPNGPLYYNGWYHFFYQYNPRAAVWGNIVWGHAVSTD 180

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHWLHLPLA+V DQW+DI+GVWTGSATILPDGRIMMLYTGST+E VQVQNLAYPANLSD
Sbjct: 181 LIHWLHLPLALVPDQWYDINGVWTGSATILPDGRIMMLYTGSTKEHVQVQNLAYPANLSD 240

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL+DW+K+ GNPV++PPPGIDFRDFRDPTTAW TSEGKWR AIGSK+N+TGISL+Y+TE
Sbjct: 241 PLLIDWVKFSGNPVLVPPPGIDFRDFRDPTTAWFTSEGKWRIAIGSKVNRTGISLVYDTE 300

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF++FQLLDNLLCAV GTGMWEC+DFFPVSK  +IGLDTSVNGP VKHVVKTSLDDDRHD
Sbjct: 301 DFKHFQLLDNLLCAVAGTGMWECLDFFPVSKDGKIGLDTSVNGPDVKHVVKTSLDDDRHD 360

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKT TWVPD+P IDVGIGLRYDYG+ YASKSF+D  K RR+LWGWIGE+DSE
Sbjct: 361 YYSLGTYDEKTATWVPDNPKIDVGIGLRYDYGLFYASKSFFDHKKGRRVLWGWIGEADSE 420

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWAS+Q IPRT+LFD KTGTNLLQWPVEE+ENLRQR+  F+NL++  GSVVPL+
Sbjct: 421 YADVQKGWASLQGIPRTVLFDNKTGTNLLQWPVEEIENLRQRSHAFHNLVIHPGSVVPLE 480

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           VG +TQLDI AEFELD+EAVAK + +++EFSCQ RGGAA RGALGPFGLLVLADE+L+EH
Sbjct: 481 VGSSTQLDIFAEFELDKEAVAKAIETNVEFSCQRRGGAAIRGALGPFGLLVLADETLSEH 540

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV KGQNGTLKT FC DESRSS ANDV+KPIYGSSVPVLE EK SLRILVDHSVV
Sbjct: 541 TPVYFYVAKGQNGTLKTFFCTDESRSSEANDVYKPIYGSSVPVLEGEKFSLRILVDHSVV 600

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CITSRVYPTKAIY  ARLFVFNNAKDTN+TAS+T+WQMN AFIRPY PG D
Sbjct: 601 ESFAQGGRTCITSRVYPTKAIYGAARLFVFNNAKDTNITASLTIWQMNPAFIRPYHPGGD 660

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLITWDVQ 688
              S+S VVL+P YLFLLVSLLHLI WDVQ
Sbjct: 661 HDSSKSCVVLLPIYLFLLVSLLHLIKWDVQ 684

BLAST of MC06g1327 vs. NCBI nr
Match: KAA0039686.1 (acid beta-fructofuranosidase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 529/685 (77.23%), Postives = 594/685 (86.72%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MVN NPF AFF SG  P +LP YSPLP +P   ++ P  +RRP+K  +LI+SGL LV LL
Sbjct: 1   MVNFNPFPAFFASGHQPENLPFYSPLPGDP---LEYPAFQRRPVKRTLLISSGLFLVCLL 60

Query: 61  IAVIGQNGVVFYAKLEGSS--SSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+I Q  V F A L G     S+ PE++ P SRGVSAGVSEKANR F+GQN++ FPWN+
Sbjct: 61  VAIIVQTNVNFVATLPGLLFLRSQSPEILRPGSRGVSAGVSEKANRHFIGQNVAYFPWNN 120

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPEENWMNG      PLY+ GWYHFFYQYNP  AVWG+I WGHAVS D
Sbjct: 121 SMLSWQRTAFHFQPEENWMNG------PLYYNGWYHFFYQYNPRAAVWGNIVWGHAVSTD 180

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHW HLPLA+V DQW+DI+GVWTGSATILPDG+IMMLYTGST+E VQVQNLAYPANLSD
Sbjct: 181 LIHWFHLPLALVPDQWYDINGVWTGSATILPDGQIMMLYTGSTKEHVQVQNLAYPANLSD 240

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL++W+K+ GNPV++PPPGIDFRDFRDPTTAW TSEGKWR AIGSK+N+TGISL+Y+TE
Sbjct: 241 PLLINWVKFSGNPVLVPPPGIDFRDFRDPTTAWFTSEGKWRIAIGSKVNRTGISLVYDTE 300

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF++FQLLDNLLCAV GTGMWEC+DFFPVSK   +GLDTSVNGP VKHVVKTSLDDDRHD
Sbjct: 301 DFKHFQLLDNLLCAVAGTGMWECLDFFPVSKAGNVGLDTSVNGPDVKHVVKTSLDDDRHD 360

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKT TWVPDDP IDVGIGLRYDYGI YASKSF+D  K RR+LWGWIGESDSE
Sbjct: 361 YYSLGTYDEKTATWVPDDPKIDVGIGLRYDYGIFYASKSFFDHKKGRRVLWGWIGESDSE 420

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWASVQ IPRTILFD KTGT+LLQWPVEE+E+LRQR+  FNNL++Q GSVVPL+
Sbjct: 421 FADVQKGWASVQGIPRTILFDNKTGTHLLQWPVEEIESLRQRSHAFNNLVIQPGSVVPLE 480

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           +G ++QLDI AEFELD+EAVAK + +D+EFSCQTRGGAA RGALGPFGLLVLADESL+EH
Sbjct: 481 IGSSSQLDIFAEFELDKEAVAKAIETDVEFSCQTRGGAANRGALGPFGLLVLADESLSEH 540

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV KGQNGTLKT FC DESRSS ANDVFKPIYGS VPVLEDEK SLRILVDHSVV
Sbjct: 541 TPVYFYVAKGQNGTLKTFFCTDESRSSEANDVFKPIYGSYVPVLEDEKFSLRILVDHSVV 600

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CITSRVYPTKAIY  ARLFVFNNAKDTN+TAS+T+WQMN AFIRPY P + 
Sbjct: 601 ESFAQGGRTCITSRVYPTKAIYGAARLFVFNNAKDTNITASLTIWQMNPAFIRPYHPDDH 660

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLI 683
              S+SSVVL+P YLFLLVSLLHLI
Sbjct: 661 RNSSKSSVVLLPIYLFLLVSLLHLI 676

BLAST of MC06g1327 vs. NCBI nr
Match: XP_023549707.1 (acid beta-fructofuranosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1102 bits (2849), Expect = 0.0
Identity = 526/688 (76.45%), Postives = 601/688 (87.35%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MV LNPFSAF  + DP  +LP Y+PLP EP + V SP IRRRP K  V I +GLLLVGLL
Sbjct: 69  MVKLNPFSAFPATADPQQNLPFYNPLPHEPVEPVQSPPIRRRPFKGGVYIFTGLLLVGLL 128

Query: 61  IAVIGQNGVVFYAKLEGSSS--SEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+I QNG+   +KL+G+S   S+ P++  P+SRGVS GVSEKAN QF+G++++ FPWN+
Sbjct: 129 VAIICQNGMGPASKLQGNSYLISKSPKIFRPSSRGVSVGVSEKANPQFLGRSVTSFPWNN 188

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPE+NWMN   +  GPLYH GWYHFFYQYNP  AVWG+I WGHAVSRD
Sbjct: 189 SMLSWQRTAFHFQPEQNWMN---DPNGPLYHMGWYHFFYQYNPQAAVWGNIVWGHAVSRD 248

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHWLHLP+A++ D W+DI+GVWTGSAT+LPDG+IMMLYTGST+ES+QVQNLAYPANLSD
Sbjct: 249 LIHWLHLPIALLPDHWYDINGVWTGSATVLPDGQIMMLYTGSTKESIQVQNLAYPANLSD 308

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL+DW+K+PGNPV++PPPGIDF+DFRDPTTAWLTSEGKWR A+GSK+N+TGISL+YETE
Sbjct: 309 PLLIDWVKFPGNPVLVPPPGIDFKDFRDPTTAWLTSEGKWRLAVGSKVNRTGISLLYETE 368

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF+ F LLDNLLCAV  TGMWECVDFFPVSKTE  GLDTS NGP VKHV+KTSLDDDRHD
Sbjct: 369 DFQRFHLLDNLLCAVSDTGMWECVDFFPVSKTENYGLDTSANGPEVKHVMKTSLDDDRHD 428

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKTGTW+PDDP IDVGIGLRYDYGI YASKSFYD +K RR+LWGWIGESDSE
Sbjct: 429 YYSLGTYDEKTGTWIPDDPKIDVGIGLRYDYGIFYASKSFYDHNKGRRVLWGWIGESDSE 488

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWASVQSIPRTILFD KTGTNLLQWPVEEVE+LRQ +  FNNL+V+AGSV+PLD
Sbjct: 489 FADVQKGWASVQSIPRTILFDNKTGTNLLQWPVEEVESLRQESYVFNNLVVKAGSVLPLD 548

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           VG ATQLDI AEFELD+EA+ K   +++EFSCQT GGA +RGALGPFGL +LADES +EH
Sbjct: 549 VGTATQLDISAEFELDKEALEKATETNVEFSCQTSGGATERGALGPFGLSILADESRSEH 608

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV K QNG+LKT FC DESRSS A+DVFKPIYGS+VPVLEDEK +LRILVDHSVV
Sbjct: 609 TPVYFYVAKDQNGSLKTFFCTDESRSSEASDVFKPIYGSAVPVLEDEKFTLRILVDHSVV 668

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CITSRVYPTKAIY  ARLFVFNNA+DT+VTASITVWQMN A IRPY PG D
Sbjct: 669 ESFAQGGRTCITSRVYPTKAIYGAARLFVFNNAEDTDVTASITVWQMNPALIRPYRPGGD 728

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLITWD 686
              S++SVVL+PFYLFLLVSLLHLI + 
Sbjct: 729 DS-SKNSVVLIPFYLFLLVSLLHLIVYK 752

BLAST of MC06g1327 vs. ExPASy TrEMBL
Match: A0A6J1DUZ6 (Beta-fructofuranosidase OS=Momordica charantia OX=3673 GN=LOC111023337 PE=3 SV=1)

HSP 1 Score: 1394 bits (3608), Expect = 0.0
Identity = 682/689 (98.98%), Postives = 683/689 (99.13%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL
Sbjct: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60

Query: 61  IAVIGQNGVVFYAKLEGSSSSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSM 120
           IAVIGQNGVVFYAKLEGSSSSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSM
Sbjct: 61  IAVIGQNGVVFYAKLEGSSSSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNHSM 120

Query: 121 LMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMI 180
           LMWQRTAFHFQPEENWMN   +  GPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMI
Sbjct: 121 LMWQRTAFHFQPEENWMN---DPNGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMI 180

Query: 181 HWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPL 240
           HWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPL
Sbjct: 181 HWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPL 240

Query: 241 LVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDF 300
           LVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDF
Sbjct: 241 LVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDF 300

Query: 301 ENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYY 360
           ENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYY
Sbjct: 301 ENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYY 360

Query: 361 SLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVS 420
           SLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVS
Sbjct: 361 SLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVS 420

Query: 421 DVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVG 480
           DVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVG
Sbjct: 421 DVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVG 480

Query: 481 KATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTP 540
           KATQLDILAEFELDEEAVAKVV SDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTP
Sbjct: 481 KATQLDILAEFELDEEAVAKVVXSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEHTP 540

Query: 541 IYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVES 600
           IYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVES
Sbjct: 541 IYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVES 600

Query: 601 FAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSK 660
           FAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSK
Sbjct: 601 FAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGEDSK 660

Query: 661 KSQSSVVLVPFYLFLLVSLLHLITWDVQG 689
           KSQSSVVLVPFYLFLLVSLLHLITWDVQG
Sbjct: 661 KSQSSVVLVPFYLFLLVSLLHLITWDVQG 686

BLAST of MC06g1327 vs. ExPASy TrEMBL
Match: A0A0A0KKK0 (Beta-fructofuranosidase OS=Cucumis sativus OX=3659 GN=Csa_5G160230 PE=3 SV=1)

HSP 1 Score: 1108 bits (2865), Expect = 0.0
Identity = 531/690 (76.96%), Postives = 597/690 (86.52%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MV  N F  FF SG  P +LP YSPLP +P Q   SP I+RRP+K  +LI+SG  LV LL
Sbjct: 1   MVTFNHFPTFFASGHHPENLPFYSPLPGDPLQ---SPSIQRRPVKRTLLISSGFFLVCLL 60

Query: 61  IAVIGQNGVVFYAKLEGSS--SSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+I QN V F A   G S  SS+ PE++ P SRGVS GVSEKANR F+GQN++ FPWN+
Sbjct: 61  VAIIVQNNVDFVATFPGLSFLSSKSPEILPPVSRGVSEGVSEKANRHFIGQNLAYFPWNN 120

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPEENWMN   +  GPLY+ GWYHFFYQYNP  AVWG+I WGHAVS D
Sbjct: 121 SMLSWQRTAFHFQPEENWMN---DPNGPLYYNGWYHFFYQYNPRAAVWGNIVWGHAVSTD 180

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHWLHLPLA+V DQW+DI+GVWTGSATILPDGRIMMLYTGST+E VQVQNLAYPANLSD
Sbjct: 181 LIHWLHLPLALVPDQWYDINGVWTGSATILPDGRIMMLYTGSTKEHVQVQNLAYPANLSD 240

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL+DW+K+ GNPV++PPPGIDFRDFRDPTTAW TSEGKWR AIGSK+N+TGISL+Y+TE
Sbjct: 241 PLLIDWVKFSGNPVLVPPPGIDFRDFRDPTTAWFTSEGKWRIAIGSKVNRTGISLVYDTE 300

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF++FQLLDNLLCAV GTGMWEC+DFFPVSK  +IGLDTSVNGP VKHVVKTSLDDDRHD
Sbjct: 301 DFKHFQLLDNLLCAVAGTGMWECLDFFPVSKDGKIGLDTSVNGPDVKHVVKTSLDDDRHD 360

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKT TWVPD+P IDVGIGLRYDYG+ YASKSF+D  K RR+LWGWIGE+DSE
Sbjct: 361 YYSLGTYDEKTATWVPDNPKIDVGIGLRYDYGLFYASKSFFDHKKGRRVLWGWIGEADSE 420

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWAS+Q IPRT+LFD KTGTNLLQWPVEE+ENLRQR+  F+NL++  GSVVPL+
Sbjct: 421 YADVQKGWASLQGIPRTVLFDNKTGTNLLQWPVEEIENLRQRSHAFHNLVIHPGSVVPLE 480

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           VG +TQLDI AEFELD+EAVAK + +++EFSCQ RGGAA RGALGPFGLLVLADE+L+EH
Sbjct: 481 VGSSTQLDIFAEFELDKEAVAKAIETNVEFSCQRRGGAAIRGALGPFGLLVLADETLSEH 540

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV KGQNGTLKT FC DESRSS ANDV+KPIYGSSVPVLE EK SLRILVDHSVV
Sbjct: 541 TPVYFYVAKGQNGTLKTFFCTDESRSSEANDVYKPIYGSSVPVLEGEKFSLRILVDHSVV 600

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CITSRVYPTKAIY  ARLFVFNNAKDTN+TAS+T+WQMN AFIRPY PG D
Sbjct: 601 ESFAQGGRTCITSRVYPTKAIYGAARLFVFNNAKDTNITASLTIWQMNPAFIRPYHPGGD 660

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLITWDVQ 688
              S+S VVL+P YLFLLVSLLHLI WDVQ
Sbjct: 661 HDSSKSCVVLLPIYLFLLVSLLHLIKWDVQ 684

BLAST of MC06g1327 vs. ExPASy TrEMBL
Match: A0A5A7TCA4 (Beta-fructofuranosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00170 PE=3 SV=1)

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 529/685 (77.23%), Postives = 594/685 (86.72%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MVN NPF AFF SG  P +LP YSPLP +P   ++ P  +RRP+K  +LI+SGL LV LL
Sbjct: 1   MVNFNPFPAFFASGHQPENLPFYSPLPGDP---LEYPAFQRRPVKRTLLISSGLFLVCLL 60

Query: 61  IAVIGQNGVVFYAKLEGSS--SSEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+I Q  V F A L G     S+ PE++ P SRGVSAGVSEKANR F+GQN++ FPWN+
Sbjct: 61  VAIIVQTNVNFVATLPGLLFLRSQSPEILRPGSRGVSAGVSEKANRHFIGQNVAYFPWNN 120

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPEENWMNG      PLY+ GWYHFFYQYNP  AVWG+I WGHAVS D
Sbjct: 121 SMLSWQRTAFHFQPEENWMNG------PLYYNGWYHFFYQYNPRAAVWGNIVWGHAVSTD 180

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHW HLPLA+V DQW+DI+GVWTGSATILPDG+IMMLYTGST+E VQVQNLAYPANLSD
Sbjct: 181 LIHWFHLPLALVPDQWYDINGVWTGSATILPDGQIMMLYTGSTKEHVQVQNLAYPANLSD 240

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL++W+K+ GNPV++PPPGIDFRDFRDPTTAW TSEGKWR AIGSK+N+TGISL+Y+TE
Sbjct: 241 PLLINWVKFSGNPVLVPPPGIDFRDFRDPTTAWFTSEGKWRIAIGSKVNRTGISLVYDTE 300

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF++FQLLDNLLCAV GTGMWEC+DFFPVSK   +GLDTSVNGP VKHVVKTSLDDDRHD
Sbjct: 301 DFKHFQLLDNLLCAVAGTGMWECLDFFPVSKAGNVGLDTSVNGPDVKHVVKTSLDDDRHD 360

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKT TWVPDDP IDVGIGLRYDYGI YASKSF+D  K RR+LWGWIGESDSE
Sbjct: 361 YYSLGTYDEKTATWVPDDPKIDVGIGLRYDYGIFYASKSFFDHKKGRRVLWGWIGESDSE 420

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWASVQ IPRTILFD KTGT+LLQWPVEE+E+LRQR+  FNNL++Q GSVVPL+
Sbjct: 421 FADVQKGWASVQGIPRTILFDNKTGTHLLQWPVEEIESLRQRSHAFNNLVIQPGSVVPLE 480

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           +G ++QLDI AEFELD+EAVAK + +D+EFSCQTRGGAA RGALGPFGLLVLADESL+EH
Sbjct: 481 IGSSSQLDIFAEFELDKEAVAKAIETDVEFSCQTRGGAANRGALGPFGLLVLADESLSEH 540

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV KGQNGTLKT FC DESRSS ANDVFKPIYGS VPVLEDEK SLRILVDHSVV
Sbjct: 541 TPVYFYVAKGQNGTLKTFFCTDESRSSEANDVFKPIYGSYVPVLEDEKFSLRILVDHSVV 600

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CITSRVYPTKAIY  ARLFVFNNAKDTN+TAS+T+WQMN AFIRPY P + 
Sbjct: 601 ESFAQGGRTCITSRVYPTKAIYGAARLFVFNNAKDTNITASLTIWQMNPAFIRPYHPDDH 660

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLI 683
              S+SSVVL+P YLFLLVSLLHLI
Sbjct: 661 RNSSKSSVVLLPIYLFLLVSLLHLI 676

BLAST of MC06g1327 vs. ExPASy TrEMBL
Match: A0A6J1I3W9 (Beta-fructofuranosidase OS=Cucurbita maxima OX=3661 GN=LOC111468937 PE=3 SV=1)

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 524/688 (76.16%), Postives = 598/688 (86.92%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MV LNPFS F  + DPP +LP Y+PLP EP + V+SP IRRRP K  + I +GLLLVGL 
Sbjct: 1   MVKLNPFSTFPATADPPQNLPFYNPLPHEPVEPVESPPIRRRPFKGGLYIFTGLLLVGLS 60

Query: 61  IAVIGQNGVVFYAKLEGSSS--SEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+I QNG+   AKL+G+S   S+ P+++ P+SRGVSAGVSEKAN QF+G+ ++ FPWN+
Sbjct: 61  VALICQNGLGPVAKLQGNSLLISKSPKILRPSSRGVSAGVSEKANPQFLGRRVASFPWNN 120

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPE+NWMN   +  GPLYH GWYHFFYQYNP  AVWG+I WGHAVSRD
Sbjct: 121 SMLSWQRTAFHFQPEQNWMN---DPNGPLYHMGWYHFFYQYNPQAAVWGNIVWGHAVSRD 180

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHWLHLPLA++ D W+DI+GVWTGSAT+LPDG+IMMLYTGST+ES+QVQNLAYPANLSD
Sbjct: 181 LIHWLHLPLALLPDHWYDINGVWTGSATVLPDGQIMMLYTGSTKESIQVQNLAYPANLSD 240

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL+DW+K+PGNPV++PPPGIDF+DFRDPTTAWLTSEGKWR A+GSK+N+TGISL+YETE
Sbjct: 241 PLLIDWVKFPGNPVLVPPPGIDFKDFRDPTTAWLTSEGKWRLAVGSKVNRTGISLLYETE 300

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF+ F LLDNLLCAV  TGMWECVDFFPVSKTE  GLDTS NGP VKHV+KTSLDDDRHD
Sbjct: 301 DFQRFHLLDNLLCAVSDTGMWECVDFFPVSKTENYGLDTSANGPEVKHVMKTSLDDDRHD 360

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKTGTW+PDDP IDVGIGLRYDYGI YASKSFYD +K RR+LWGWIGESDSE
Sbjct: 361 YYSLGTYDEKTGTWIPDDPKIDVGIGLRYDYGIFYASKSFYDHNKGRRVLWGWIGESDSE 420

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWASVQSIPRTILFD KTGTNLLQWPVEEVE+LRQ +  FNNL+V+AGSV+PLD
Sbjct: 421 FADVQKGWASVQSIPRTILFDNKTGTNLLQWPVEEVESLRQESHVFNNLVVKAGSVLPLD 480

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           VG ATQLDI AEF+LD+EA+ K   +++EFSCQT GGA +RGALGPFGL +LADES +EH
Sbjct: 481 VGIATQLDISAEFKLDKEALEKATKTNVEFSCQTSGGATERGALGPFGLSILADESRSEH 540

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV K QNG+LKT FC DESRSS A+DVFKPIYGS+VPVLEDEK +LRILVDHSVV
Sbjct: 541 TPVYFYVAKDQNGSLKTFFCTDESRSSEASDVFKPIYGSAVPVLEDEKFTLRILVDHSVV 600

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CITSRVYPTKAIY  ARLFVFNNAKD +V ASITVWQMN A IRPY PG D
Sbjct: 601 ESFAQGGRTCITSRVYPTKAIYGAARLFVFNNAKDIDVIASITVWQMNPALIRPYRPGGD 660

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLITWD 686
              S+SSVVL+P YLFLLVS LHLI + 
Sbjct: 661 DDSSKSSVVLIPVYLFLLVSFLHLIVYK 685

BLAST of MC06g1327 vs. ExPASy TrEMBL
Match: A0A6J1E2Q4 (Beta-fructofuranosidase OS=Cucurbita moschata OX=3662 GN=LOC111430259 PE=3 SV=1)

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 528/688 (76.74%), Postives = 597/688 (86.77%), Query Frame = 0

Query: 1   MVNLNPFSAFFGSGDPPGSLPVYSPLPDEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLL 60
           MV LNPFSAF  + D P +LP Y+PLP EP + V SP IRRRP K  V I +GLLLVGLL
Sbjct: 1   MVKLNPFSAFPVTADTPQNLPFYNPLPHEPVEPVQSPPIRRRPFKGGVYIFTGLLLVGLL 60

Query: 61  IAVIGQNGVVFYAKLEGSSS--SEPPEMVCPASRGVSAGVSEKANRQFVGQNISPFPWNH 120
           +A+I QNG+   AKL+G+S   S+ P++  P+SRGVS GVSEKAN QF+G+ ++ FPWN+
Sbjct: 61  VAIICQNGLGPAAKLQGNSYLISKSPKIFRPSSRGVSVGVSEKANPQFLGRTLASFPWNN 120

Query: 121 SMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRD 180
           SML WQRTAFHFQPE+NWMN   +  GPLYH GWYHFFYQYNP  AVWG+I WGHAVSRD
Sbjct: 121 SMLSWQRTAFHFQPEQNWMN---DPNGPLYHMGWYHFFYQYNPQAAVWGNIVWGHAVSRD 180

Query: 181 MIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSD 240
           +IHWLHLP+A++ D W+DI+GVWTGSAT+LPDG+IMMLYTGST+ES+QVQNLAYPANLSD
Sbjct: 181 LIHWLHLPIALLPDHWYDINGVWTGSATVLPDGQIMMLYTGSTKESIQVQNLAYPANLSD 240

Query: 241 PLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETE 300
           PLL+DW+K+PGNPV++PPPGIDF+DFRDPTTAWLTSEGKWR A+GSK+N+TGISL+YETE
Sbjct: 241 PLLIDWVKFPGNPVLVPPPGIDFKDFRDPTTAWLTSEGKWRLAVGSKVNRTGISLLYETE 300

Query: 301 DFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHD 360
           DF+ F LLDNLLCAV  TGMWECVDFFPVSKTE  GLDTS NGP VKHV+KTSLDDDRHD
Sbjct: 301 DFQRFHLLDNLLCAVSDTGMWECVDFFPVSKTENYGLDTSANGPEVKHVMKTSLDDDRHD 360

Query: 361 YYSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSE 420
           YYSLGTYDEKTGTW+PDDP  DVGIGLRYDYGI YASKSFYD +K RR+LWGWIGESDSE
Sbjct: 361 YYSLGTYDEKTGTWIPDDPKTDVGIGLRYDYGIFYASKSFYDHNKGRRVLWGWIGESDSE 420

Query: 421 VSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLD 480
            +DVQKGWASVQSIPRTILFD KTGTNLLQWPVEEVE+LRQ +  FNNL+V+AGSV+PLD
Sbjct: 421 FADVQKGWASVQSIPRTILFDNKTGTNLLQWPVEEVESLRQESHVFNNLVVKAGSVLPLD 480

Query: 481 VGKATQLDILAEFELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESLTEH 540
           VG ATQLDI AEFELD+EA+ K   +++EFSCQT GGA KRGALGPFGL +LADES +EH
Sbjct: 481 VGIATQLDISAEFELDKEALEKATETNVEFSCQTSGGATKRGALGPFGLSILADESRSEH 540

Query: 541 TPIYFYVGKGQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVV 600
           TP+YFYV K QNG+LKT FC DESRSS A+DVFKPIYGS+VPVLEDEK +LRILVDHSVV
Sbjct: 541 TPVYFYVAKDQNGSLKTFFCTDESRSSEASDVFKPIYGSAVPVLEDEKFTLRILVDHSVV 600

Query: 601 ESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYSPGED 660
           ESFAQGGR+CITSRVYPTKAIY  ARLFVFNNA DTNVTASITVWQMN A IRPY PG D
Sbjct: 601 ESFAQGGRTCITSRVYPTKAIYGAARLFVFNNALDTNVTASITVWQMNPALIRPYRPGGD 660

Query: 661 SKKSQSSVVLVPFYLFLLVSLLHLITWD 686
              S+SSVV +PFYLFLLVSLLHLI + 
Sbjct: 661 DS-SKSSVVFIPFYLFLLVSLLHLIIYK 684

BLAST of MC06g1327 vs. TAIR 10
Match: AT1G12240.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 834.7 bits (2155), Expect = 5.3e-242
Identity = 410/647 (63.37%), Postives = 496/647 (76.66%), Query Frame = 0

Query: 23  YSPLP-DEPAQTVDSPVIRRRPIKEAVLIASGLLLVGLLIAVIGQNGVVFYAKLEGSSSS 82
           Y+ LP  +P Q    P  RRRP K  + ++ GLL +   +A+I        A  +GS S+
Sbjct: 21  YTRLPMADPNQETHGP-RRRRPFKGLLAVSFGLLFIAFYVALI--------ATHDGSRSN 80

Query: 83  EP--PEMVCPASRGVSAGVSEKAN----RQFVGQNISPFPWNHSMLMWQRTAFHFQPEEN 142
           +    E     SR   AGVSEK N    +    +N   F WN+SML WQRTAFHFQPE+N
Sbjct: 81  DEGIDETETITSRARLAGVSEKRNDGLWKLSGDRNTPAFEWNNSMLSWQRTAFHFQPEQN 140

Query: 143 WMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLHLPLAIVADQWF 202
           WMN   +  GPL++KGWYHFFYQYNP  AVWGDI WGHAVSRD+IHW+HLP+A+VADQW+
Sbjct: 141 WMN---DPNGPLFYKGWYHFFYQYNPNAAVWGDIVWGHAVSRDLIHWVHLPIAMVADQWY 200

Query: 203 DISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDWIKYPGNPVILP 262
           D +GVWTGSAT LPDG I+MLYTGST ++VQVQNLAYP + +DPLL+ W+K+PGNPV++P
Sbjct: 201 DSNGVWTGSATFLPDGSIVMLYTGSTDKAVQVQNLAYPEDPNDPLLLKWVKFPGNPVLVP 260

Query: 263 PPGIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDFENFQLLDNLLCAVPG 322
           PPGI  +DFRDPTTAW TSEGKWR  IGSK+N+TGISL+Y+T DF+ ++ LD LL  VP 
Sbjct: 261 PPGILPKDFRDPTTAWKTSEGKWRITIGSKLNKTGISLVYDTIDFKTYEKLDTLLHRVPN 320

Query: 323 TGMWECVDFFPVSKTEEIGLDTSVNGPAVKHVVKTSLDDDRHDYYSLGTYDEKTGTWVPD 382
           TGMWECVDF+PVSKT   GLDTSVNGP VKH+VK S+DD R D+Y++GTY +  GTW+PD
Sbjct: 321 TGMWECVDFYPVSKTAGNGLDTSVNGPDVKHIVKASMDDTRFDHYAVGTYFDSNGTWIPD 380

Query: 383 DPTIDVGI--GLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVSDVQKGWASVQSIP 442
           DPTIDVG+   LRYDYG  YASKSFYDQ+K RR+LW WIGESDSE SDVQKGW+S+Q IP
Sbjct: 381 DPTIDVGMTASLRYDYGKFYASKSFYDQNKGRRVLWSWIGESDSEASDVQKGWSSLQGIP 440

Query: 443 RTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVGKATQLDILAEFEL 502
           RT++ D KTG NL+QWPVEE+++LR  +  F +L V  GSVVP+DVG A QLDI AEFE+
Sbjct: 441 RTVVLDTKTGKNLVQWPVEEIKSLRLSSKQF-DLEVGPGSVVPVDVGSAAQLDIEAEFEI 500

Query: 503 DEEAVAKVVGSDI------EFSCQTRGGAAKRGALGPFGLLVLADESLTEHTPIYFYVGK 562
           ++E++ K++G+        EFSC+  GG+  RGALGPFG  VLA ESL+E TP+YFYV K
Sbjct: 501 NKESLDKIIGNASVVAEAEEFSCEKSGGSTVRGALGPFGFSVLATESLSEQTPVYFYVAK 560

Query: 563 GQNGTLKTCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILVDHSVVESFAQGGRS 622
           G++  LKT FC D SRSSVANDV KPIYGS VPVL+ EKL++RILVDHS+VE+F QGGR+
Sbjct: 561 GKDSELKTFFCTDTSRSSVANDVVKPIYGSVVPVLKGEKLTMRILVDHSIVEAFGQGGRT 620

Query: 623 CITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFIRPYS 655
           CITSRVYPT AIY  A+LF+FNNA D  VTAS TVWQMNSAFI PYS
Sbjct: 621 CITSRVYPTTAIYGAAKLFLFNNALDATVTASFTVWQMNSAFIHPYS 654

BLAST of MC06g1327 vs. TAIR 10
Match: AT1G62660.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 814.3 bits (2102), Expect = 7.4e-236
Identity = 392/626 (62.62%), Postives = 480/626 (76.68%), Query Frame = 0

Query: 40  RRRPIKEAVLIASGLLLVGLLIAVIGQNGVVFYAKLEGSSSSEPPEMVCPASRGVSAGVS 99
           RRRPIK  + + SGLLL+ L + +I  +        +GS +      +   SR   AGVS
Sbjct: 30  RRRPIKVHLAVYSGLLLIALYVTLIVTH--------DGSKAE-----IATESRPRMAGVS 89

Query: 100 EKANR----QFVGQNISPFPWNHSMLMWQRTAFHFQPEENWMNGFENVTGPLYHKGWYHF 159
           EK+N           +  FPWN+++L WQRTAFHFQPE+NWMN   +  GPL++KGWYHF
Sbjct: 90  EKSNDGVWISSDDGKVEAFPWNNTILSWQRTAFHFQPEKNWMN---DPNGPLFYKGWYHF 149

Query: 160 FYQYNPYDAVWGDISWGHAVSRDMIHWLHLPLAIVADQWFDISGVWTGSATILPDGRIMM 219
           FYQYNP  AVWGDI WGHAVS+D+IHWL+LP+A+V DQW+D +GVWTGSAT L DG I+M
Sbjct: 150 FYQYNPNAAVWGDIVWGHAVSKDLIHWLYLPIAMVPDQWYDANGVWTGSATFLDDGSIVM 209

Query: 220 LYTGSTQESVQVQNLAYPANLSDPLLVDWIKYPGNPVILPPPGIDFRDFRDPTTAWLTSE 279
           LYTGST E VQVQNLAYP + SDPLL+ W+K+ GNPV++PPPGI  +DFRDPTTAW TS 
Sbjct: 210 LYTGSTDEFVQVQNLAYPEDPSDPLLLKWVKFSGNPVLVPPPGIGAKDFRDPTTAWKTSS 269

Query: 280 GKWRFAIGSKINQTGISLMYETEDFENFQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGL 339
           GKWR  IGSKIN+TGISL+Y+T DF+ ++  + LL  VP TGMWECVDF+PVSKT+  GL
Sbjct: 270 GKWRITIGSKINRTGISLIYDTTDFKTYEKHETLLHQVPNTGMWECVDFYPVSKTQLNGL 329

Query: 340 DTSVNGPAVKHVVKTSLDDDRHDYYSLGTYDEKTGTWVPDDPTIDVGI--GLRYDYGILY 399
           DTSVNGP VKHV+K S+DD R D+Y++GTYD+   TWVPD+P+IDVGI  GLRYDYG  Y
Sbjct: 330 DTSVNGPDVKHVIKASMDDTRIDHYAIGTYDDSNATWVPDNPSIDVGISTGLRYDYGKYY 389

Query: 400 ASKSFYDQHKRRRLLWGWIGESDSEVSDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEE 459
           ASK+FYDQ+K RR+LWGWIGESDSE +DVQKGW+SVQ IPRT++ D +T  NL+QWPVEE
Sbjct: 390 ASKTFYDQNKGRRILWGWIGESDSEAADVQKGWSSVQGIPRTVVLDTRTHKNLVQWPVEE 449

Query: 460 VENLRQRNDTFNNLLVQAGSVVPLDVGKATQLDILAEFELDEEAVA-----KVVGSDIEF 519
           +++LR  +  F ++ +  G+VVP+DVG ATQLDI AEFE+  + +        V +D +F
Sbjct: 450 IKSLRLSSKKF-DMTIGPGTVVPVDVGSATQLDIEAEFEIKTDDLKLFFDDDSVEADNKF 509

Query: 520 SCQTRGGAAKRGALGPFGLLVLADESLTEHTPIYFYVGKGQNGTLKTCFCADESRSSVAN 579
           SC+T GG+  RGALGPFG  VLADE L+E TP+YFYV KG++  L T FC D SRS++AN
Sbjct: 510 SCETNGGSTARGALGPFGFSVLADEGLSEQTPVYFYVTKGKHSKLNTVFCTDTSRSTLAN 569

Query: 580 DVFKPIYGSSVPVLEDEKLSLRILVDHSVVESFAQGGRSCITSRVYPTKAIYNDARLFVF 639
           DV KPIYGS VPVL+ EKL++RILVDHS+VE FAQGGRSCITSRVYPTKAIY   +LF+F
Sbjct: 570 DVVKPIYGSFVPVLKGEKLTMRILVDHSIVEGFAQGGRSCITSRVYPTKAIYGATKLFLF 629

Query: 640 NNAKDTNVTASITVWQMNSAFIRPYS 655
           NNA D  VTAS TVWQMN+AFI PYS
Sbjct: 630 NNAIDATVTASFTVWQMNNAFIHPYS 638

BLAST of MC06g1327 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 475.7 bits (1223), Expect = 6.3e-134
Identity = 248/537 (46.18%), Postives = 337/537 (62.76%), Query Frame = 0

Query: 125 RTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLH 184
           RT FHFQP +NWMN   +  GP+ +KG YH FYQ+NP  AVWG+I W H+ S D+I+W  
Sbjct: 52  RTGFHFQPPKNWMN---DPNGPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDP 111

Query: 185 LPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDW 244
            P AI     FDI+G W+GSATILP+G+ ++LYTG   ++ QVQN+A P NLSDP L +W
Sbjct: 112 HPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREW 171

Query: 245 IKYPGNPVILPPP--GIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDFEN 304
            K P NP++ P    GI+   FRDPTTAWL  + KWR  IGSKI++ G+++ Y ++DF  
Sbjct: 172 KKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLK 231

Query: 305 FQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNG---PAVKHVVKTSLDDDRHDY 364
           ++     L    G+GMWEC DFFPV++    G++TS  G     +KHV+K SLDD +HDY
Sbjct: 232 WEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDY 291

Query: 365 YSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEV 424
           Y++GTYD     +VPD+     G   RYDYG  YASK+F+D  K RR+LWGW  ES S  
Sbjct: 292 YTIGTYDRVKDKFVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVE 351

Query: 425 SDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRN-DTFNNLLVQAGSVVPLD 484
            DV+KGW+ +Q+IPR I  D ++G  L+QWPV EVE LR +      N ++++GS + + 
Sbjct: 352 DDVEKGWSGIQTIPRKIWLD-RSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVY 411

Query: 485 VGKATQLDILAEF---ELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESL 544
              A Q D+   F   +L++  V +   +D +  C     + K G LGPFGL+VLA ++L
Sbjct: 412 GVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSG-LGPFGLMVLASKNL 471

Query: 545 TEHTPIYFYVGKGQNGTLK--TCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILV 604
            E+T +YF + K +  + K     C+D+SRSS+  D  K  YG+ V +   + LSLR L+
Sbjct: 472 EEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINPHQPLSLRALI 531

Query: 605 DHSVVESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFI 651
           DHSVVESF   GR+CITSRVYP  AI   + LF FN    +    ++  W MNSA I
Sbjct: 532 DHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 583

BLAST of MC06g1327 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.8e-133
Identity = 248/537 (46.18%), Postives = 336/537 (62.57%), Query Frame = 0

Query: 125 RTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLH 184
           RT FHFQP +NWMN      GP+ +KG YH FYQ+NP  AVWG+I W H+ S D+I+W  
Sbjct: 52  RTGFHFQPPKNWMN------GPMIYKGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDP 111

Query: 185 LPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDW 244
            P AI     FDI+G W+GSATILP+G+ ++LYTG   ++ QVQN+A P NLSDP L +W
Sbjct: 112 HPPAIFPSAPFDINGCWSGSATILPNGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREW 171

Query: 245 IKYPGNPVILPPP--GIDFRDFRDPTTAWLTSEGKWRFAIGSKINQTGISLMYETEDFEN 304
            K P NP++ P    GI+   FRDPTTAWL  + KWR  IGSKI++ G+++ Y ++DF  
Sbjct: 172 KKSPLNPLMAPDAVNGINASSFRDPTTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLK 231

Query: 305 FQLLDNLLCAVPGTGMWECVDFFPVSKTEEIGLDTSVNG---PAVKHVVKTSLDDDRHDY 364
           ++     L    G+GMWEC DFFPV++    G++TS  G     +KHV+K SLDD +HDY
Sbjct: 232 WEKSPEPLHYDDGSGMWECPDFFPVTRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDY 291

Query: 365 YSLGTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEV 424
           Y++GTYD     +VPD+     G   RYDYG  YASK+F+D  K RR+LWGW  ES S  
Sbjct: 292 YTIGTYDRVKDKFVPDNGFKMDGTAPRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVE 351

Query: 425 SDVQKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRN-DTFNNLLVQAGSVVPLD 484
            DV+KGW+ +Q+IPR I  D ++G  L+QWPV EVE LR +      N ++++GS + + 
Sbjct: 352 DDVEKGWSGIQTIPRKIWLD-RSGKQLIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVY 411

Query: 485 VGKATQLDILAEF---ELDEEAVAKVVGSDIEFSCQTRGGAAKRGALGPFGLLVLADESL 544
              A Q D+   F   +L++  V +   +D +  C     + K G LGPFGL+VLA ++L
Sbjct: 412 GVTAAQADVEVLFKVRDLEKADVIEPSWTDPQLICSKMNVSVKSG-LGPFGLMVLASKNL 471

Query: 545 TEHTPIYFYVGKGQNGTLK--TCFCADESRSSVANDVFKPIYGSSVPVLEDEKLSLRILV 604
            E+T +YF + K +  + K     C+D+SRSS+  D  K  YG+ V +   + LSLR L+
Sbjct: 472 EEYTSVYFRIFKARQNSNKYVVLMCSDQSRSSLKEDNDKTTYGAFVDINPHQPLSLRALI 531

Query: 605 DHSVVESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQMNSAFI 651
           DHSVVESF   GR+CITSRVYP  AI   + LF FN    +    ++  W MNSA I
Sbjct: 532 DHSVVESFGGKGRACITSRVYPKLAIGKSSHLFAFNYGYQSVDVLNLNAWSMNSAQI 580

BLAST of MC06g1327 vs. TAIR 10
Match: AT3G52600.1 (cell wall invertase 2 )

HSP 1 Score: 459.9 bits (1182), Expect = 3.6e-129
Identity = 242/539 (44.90%), Postives = 320/539 (59.37%), Query Frame = 0

Query: 125 RTAFHFQPEENWMNGFENVTGPLYHKGWYHFFYQYNPYDAVWGDISWGHAVSRDMIHWLH 184
           RTA+HFQP  +W+N   +   P+ +KG YH FYQYNP  AVWG+I W H+VS+D+I+W  
Sbjct: 48  RTAYHFQPPRHWIN---DPNAPMLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEA 107

Query: 185 LPLAIVADQWFDISGVWTGSATILPDGRIMMLYTGSTQESVQVQNLAYPANLSDPLLVDW 244
           L  AI   +WFDI+G W+GSAT +P    ++LYTG T+   Q+QN A P +LSDP L  W
Sbjct: 108 LEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTW 167

Query: 245 IKYPGNPVILPPPGIDFRDFRDPTTAWLT-SEGKWRFAIGSKINQTGISLMYETEDFENF 304
           IK   NP++ P  G +   FRDPTTAW    +G WR  +GSK    GI+ MY++ DF+ +
Sbjct: 168 IKPDDNPIVKPDNGENGSAFRDPTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKW 227

Query: 305 QLLDNLLCAVPGTGMWECVDFFPVSKTE-EIGLDTSVNGPAVKHVVKTSLDDDRHDYYSL 364
                 + +   TGMWEC DFFPVS T+ + GLD S +GP  KHV+K SLD  R++YY+L
Sbjct: 228 VKSKRPIHSRKKTGMWECPDFFPVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTL 287

Query: 365 GTYDEKTGTWVPDDPTIDVGIGLRYDYGILYASKSFYDQHKRRRLLWGWIGESDSEVSDV 424
           GTYD K   + PD  T D   GLR+DYG  YASK+F+D    RR+LWGW  ESD+   D 
Sbjct: 288 GTYDTKKDRYRPDGYTPDGWDGLRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDT 347

Query: 425 QKGWASVQSIPRTILFDKKTGTNLLQWPVEEVENLRQRNDTFNNLLVQAGSVVPLDVGKA 484
            KGWA +Q IPRTIL D  +G  L+ WP+EE+E+LR +N    N  ++ G    +     
Sbjct: 348 VKGWAGIQLIPRTILLD-SSGKQLVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITP 407

Query: 485 TQLDILAEFELDEEAVAKVVGSDIEFS------CQTRGGAAKRGALGPFGLLVLADESLT 544
            Q+D+   F +    + K    D  F+      C  +G     G +GPFGL+ LA   L 
Sbjct: 408 AQVDVDVTFNVGN--LEKAEKFDESFATKPLELCNLKGSNV-NGGVGPFGLITLATSDLE 467

Query: 545 EHTPIYFYVGK-GQNGTLKTCFCADESRSSVAND--------VFKPIYGSSVPV-LEDEK 604
           E+TP++F V K   +   K   C+D   SS+  D        ++KP +   V V L D K
Sbjct: 468 EYTPVFFRVFKDAASNKPKVLMCSDAKPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGK 527

Query: 605 LSLRILVDHSVVESFAQGGRSCITSRVYPTKAIYNDARLFVFNNAKDTNVTASITVWQM 646
           +SLR L+DHSVVESF   G++ ITSRVYPTKA+   A LFVFNN        S+  W M
Sbjct: 528 ISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNM 579